MD10G1121800.v1.1

Thaumatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006096
Physical Location & Seq
Forward (+)
19888892 .. 19891495
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1121800.v1.1.491

Sequence Viewer

Length: 825 bp
ATGGATGGTCCTCAACTCTCTGCCTCTCTCCTCACACTTCTCGCACTCTCTTTCTTCTCAGAAGTGAACTCAGCTTCTTTCAAGCTACTCAACAAGTGCCGCTACCAAGTATGGCCCGGAATACTTTCCGGCGCCAATTCGGCGCCTCTCTCCCCCACTGGCTTCGCGCTCAAGCCCGGCAAGTCCCGGACCCTCTCCGTACCCAAATCTTGGTCGGGTCGGATCTGGGCTCGGACTCTGTGCACCGAATACTCGCCCGGTAACTTCTCCTGCGTCACAGCCGACTGCGGCTCGGGAAAAATCGAGTGTGCCGGAGGCGGCGCGAAACCGCCGGCTACGTTGGCCGAGTTCACGCTCAACGGCGCCGACGGCTTGGATTTTTATGACGTCAGCTTGGTGGACGGGTACAACCTGCCGATGCTGGTGGTGCCGCGTGGAGGAACAAGAGGGGGGTGCAGCCCGACTGGGTGCCTTGTGGACTTGAACGGCAAGTGCCCCAGGGCGCTGAGGGTGCGTGAGGGAGGATGGGGGAGCGTGGCGTGTAGGAGCGCGTGCGAGGCGTTTGGAGATCCACAGTTTTGTTGTAGCGAGGGGTACGCTACGCCCGACACATGTCAACCGTCGGCGTACTCGCTGTTTTTCAAGCATGCTTGCCCACGCTCGTATAGCTACGCGTATGACGACAAGACTAGCACTTACACGTGCGCCTCCGCCGACTACGTCATCATATTCTGCCCCTTGCCTTATACGAGTCTAAAGGTGTTGGGAGCTCGAAAAGATGGGGCAGCGCTACCACTGGTTAACAAGACCATGATGTACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

275

Amino Acids

29.14

Weight (kDa)

8.43

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 31 - 246 3.4e-82 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
Acc36I ACCTGC 1 cut(s) 420
AccB1I GGYRCC 5 cut(s) 131, 142, 362, 427, 468
AccB7I CCANNNNNTGG 1 cut(s) 210
AccII CGCG 5 cut(s) 167, 323, 433, 551, 674
AciI CCGC 6 cut(s) 100, 288, 318, 329, 431, 711
AclWI GGATC 2 cut(s) 230, 563
AcoI YGGCCR 1 cut(s) 342
AcvI CACGTG 1 cut(s) 702
AcyI GRCGYC 4 cut(s) 132, 143, 363, 387
AfaI GTAC 5 cut(s) 201, 407, 596, 629, 818
AfeI AGCGCT 1 cut(s) 789
AfiI CCNNNNNNNGG 2 cut(s) 210, 437
AflIII ACRYGT 3 cut(s) 611, 672, 699
AgsI TTSAA 3 cut(s) 82, 484, 643
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 5 cut(s) 74, 85, 393, 669, 770
AluI AGCT 5 cut(s) 74, 85, 393, 669, 770
Alw21I GWGCWC 2 cut(s) 245, 772
Alw44I GTGCAC 1 cut(s) 241
AlwI GGATC 2 cut(s) 230, 563
Ama87I CYCGRG 1 cut(s) 292
Aor51HI AGCGCT 1 cut(s) 789
AoxI GGCC 2 cut(s) 113, 342
ApaLI GTGCAC 1 cut(s) 241
ApeKI GCWGC 2 cut(s) 456, 785
Asp700I GAANNNNTTC 1 cut(s) 124
AspLEI GCGC 9 cut(s) 134, 145, 169, 323, 365, 505, 551, 707, 790
AspS9I GGNCC 3 cut(s) 8, 114, 189
AsuC2I CCSGG 4 cut(s) 117, 177, 187, 258
AvaI CYCGRG 1 cut(s) 292
AvaII GGWCC 2 cut(s) 8, 189
BaeGI GKGCMC 2 cut(s) 245, 497
BanI GGYRCC 5 cut(s) 131, 142, 362, 427, 468
BanII GRGCYC 2 cut(s) 232, 772
BbrPI CACGTG 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 245, 772
BbvCI CCTCAGC 1 cut(s) 506
BbvI GCAGC 2 cut(s) 468, 797
BccI CCATC 2 cut(s) 519, 773
BceAI ACGGC 3 cut(s) 376, 385, 502
BciT130I CCWGG 1 cut(s) 499
BcnI CCSGG 4 cut(s) 117, 177, 187, 258
BfaI CTAG 1 cut(s) 690
BfoI RGCGCY 5 cut(s) 135, 146, 366, 506, 791
BfuAI ACCTGC 1 cut(s) 420
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 6 cut(s) 100, 289, 319, 431, 457, 786
BlsI GCNGC 6 cut(s) 101, 290, 320, 432, 458, 787
Bme1390I CCNGG 5 cut(s) 117, 177, 187, 258, 499
Bme18I GGWCC 2 cut(s) 8, 189
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 8, 114, 189
BmiI GGNNCC 6 cut(s) 133, 144, 191, 364, 429, 470
BmrFI CCNGG 5 cut(s) 117, 177, 187, 258, 499
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 612
Bpu10I CCTNAGC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 155
BpuMI CCSGG 4 cut(s) 117, 177, 187, 258
BsaAI YACGTR 1 cut(s) 702
BsaHI GRCGYC 4 cut(s) 132, 143, 363, 387
BsaJI CCNNGG 2 cut(s) 497, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 437
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 3 cut(s) 163, 469, 801
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 2 cut(s) 497, 498
BseGI GGATG 2 cut(s) 10, 530
BseLI CCNNNNNNNGG 2 cut(s) 210, 437
BseMII CTCAG 3 cut(s) 72, 84, 497
BseNI ACTGG 3 cut(s) 163, 469, 801
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 2 cut(s) 245, 497
BseXI GCAGC 2 cut(s) 468, 797
BsgI GTGCAG 1 cut(s) 475
Bsh1236I CGCG 5 cut(s) 167, 323, 433, 551, 674
BshFI GGCC 2 cut(s) 115, 344
BshNI GGYRCC 5 cut(s) 131, 142, 362, 427, 468
BsiHKAI GWGCWC 2 cut(s) 245, 772
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 7 cut(s) 117, 129, 177, 187, 258, 312, 332
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 2 cut(s) 210, 437
BsmFI GGGAC 1 cut(s) 169
BsnI GGCC 2 cut(s) 115, 344
BsoBI CYCGRG 1 cut(s) 292
Bsp1286I GDGCHC 4 cut(s) 232, 245, 497, 772
Bsp143I GATC 2 cut(s) 222, 568
BspACI CCGC 6 cut(s) 100, 288, 318, 329, 431, 711
BspANI GGCC 2 cut(s) 115, 344
BspCNI CTCAG 3 cut(s) 71, 83, 498
BspFNI CGCG 5 cut(s) 167, 323, 433, 551, 674
BspLI GGNNCC 6 cut(s) 133, 144, 191, 364, 429, 470
BspMI ACCTGC 1 cut(s) 420
BspPI GGATC 2 cut(s) 230, 563
BspT107I GGYRCC 5 cut(s) 131, 142, 362, 427, 468
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 3 cut(s) 163, 469, 801
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 497, 498
BssMI GATC 2 cut(s) 222, 568
BssNI GRCGYC 4 cut(s) 132, 143, 363, 387
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 2 cut(s) 576, 621
BstACI GRCGYC 4 cut(s) 132, 143, 363, 387
BstBAI YACGTR 1 cut(s) 702
BstC8I GCNNGC 4 cut(s) 333, 553, 648, 652
BstDEI CTNAG 3 cut(s) 58, 70, 506
BstF5I GGATG 2 cut(s) 10, 530
BstFNI CGCG 5 cut(s) 167, 323, 433, 551, 674
BstH2I RGCGCY 5 cut(s) 135, 146, 366, 506, 791
BstHHI GCGC 9 cut(s) 134, 145, 169, 323, 365, 505, 551, 707, 790
BstKTI GATC 2 cut(s) 225, 571
BstMBI GATC 2 cut(s) 222, 568
BstMWI GCNNNNNNNGC 7 cut(s) 140, 341, 369, 427, 511, 557, 666
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 2 cut(s) 615, 650
BstPAI GACNNNNGTC 1 cut(s) 612
BstSCI CCNGG 5 cut(s) 115, 175, 185, 256, 497
BstSLI GKGCMC 2 cut(s) 245, 497
BstUI CGCG 5 cut(s) 167, 323, 433, 551, 674
BstV1I GCAGC 2 cut(s) 468, 797
BstX2I RGATCY 2 cut(s) 222, 568
BstYI RGATCY 2 cut(s) 222, 568
BsuRI GGCC 2 cut(s) 115, 344
BtsCI GGATG 2 cut(s) 10, 530
BtsIMutI CAGTG 2 cut(s) 156, 794
BveI ACCTGC 1 cut(s) 420
Cac8I GCNNGC 4 cut(s) 333, 553, 648, 652
CfoI GCGC 9 cut(s) 134, 145, 169, 323, 365, 505, 551, 707, 790
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 3 cut(s) 8, 114, 189
CseI GACGC 1 cut(s) 262
Csp6I GTAC 5 cut(s) 200, 406, 595, 628, 817
CviAII CATG 3 cut(s) 612, 647, 811
CviQI GTAC 5 cut(s) 200, 406, 595, 628, 817
DdeI CTNAG 3 cut(s) 58, 70, 506
DinI GGCGCC 3 cut(s) 133, 144, 364
DpnI GATC 2 cut(s) 224, 570
DpnII GATC 2 cut(s) 222, 568
EaeI YGGCCR 1 cut(s) 342
EciI GGCGGA 1 cut(s) 700
Ecl136II GAGCTC 1 cut(s) 770
Eco24I GRGCYC 2 cut(s) 232, 772
Eco47I GGWCC 2 cut(s) 8, 189
Eco47III AGCGCT 1 cut(s) 789
Eco53kI GAGCTC 1 cut(s) 770
Eco72I CACGTG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 497
EcoT38I GRGCYC 2 cut(s) 232, 772
EgeI GGCGCC 3 cut(s) 133, 144, 364
EheI GGCGCC 3 cut(s) 133, 144, 364
FaeI CATG 3 cut(s) 615, 650, 814
FaiI YATR 9 cut(s) 112, 384, 613, 648, 666, 678, 728, 747, 812
FaqI GGGAC 1 cut(s) 169
FatI CATG 3 cut(s) 611, 646, 810
Fnu4HI GCNGC 6 cut(s) 100, 289, 319, 431, 457, 786
FokI GGATG 2 cut(s) 17, 537
FriOI GRGCYC 2 cut(s) 232, 772
Fsp4HI GCNGC 6 cut(s) 100, 289, 319, 431, 457, 786
FspBI CTAG 1 cut(s) 690
GlaI GCGC 9 cut(s) 133, 144, 168, 322, 364, 504, 550, 706, 789
GluI GCNGC 6 cut(s) 100, 289, 319, 431, 457, 786
HaeII RGCGCY 5 cut(s) 135, 146, 366, 506, 791
HaeIII GGCC 2 cut(s) 115, 344
HapII CCGG 7 cut(s) 117, 129, 177, 187, 258, 312, 332
HgaI GACGC 1 cut(s) 262
HhaI GCGC 9 cut(s) 134, 145, 169, 323, 365, 505, 551, 707, 790
Hin1I GRCGYC 4 cut(s) 132, 143, 363, 387
Hin1II CATG 3 cut(s) 615, 650, 814
Hin6I GCGC 9 cut(s) 132, 143, 167, 321, 363, 503, 549, 705, 788
HinP1I GCGC 9 cut(s) 132, 143, 167, 321, 363, 503, 549, 705, 788
HincII GTYRAC 2 cut(s) 617, 802
HindII GTYRAC 2 cut(s) 617, 802
HinfI GANTC 2 cut(s) 235, 751
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 7 cut(s) 117, 129, 177, 187, 258, 312, 332
Hpy166II GTNNAC 7 cut(s) 67, 243, 351, 400, 478, 617, 802
Hpy188I TCNGA 3 cut(s) 61, 222, 234
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 7 cut(s) 67, 243, 351, 400, 478, 617, 802
Hpy99I CGWCG 2 cut(s) 371, 625
HpyCH4III ACNGT 2 cut(s) 576, 621
HpyCH4IV ACGT 4 cut(s) 338, 387, 701, 720
HpyCH4V TGCA 2 cut(s) 243, 456
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 341, 369, 427, 511, 557, 666
HpyF3I CTNAG 3 cut(s) 58, 70, 506
HpySE526I ACGT 4 cut(s) 338, 387, 701, 720
Hsp92I GRCGYC 4 cut(s) 132, 143, 363, 387
Hsp92II CATG 3 cut(s) 615, 650, 814
HspAI GCGC 9 cut(s) 132, 143, 167, 321, 363, 503, 549, 705, 788
KasI GGCGCC 3 cut(s) 131, 142, 362
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 2 cut(s) 222, 568
LmnI GCTCC 3 cut(s) 531, 546, 767
Lsp1109I GCAGC 2 cut(s) 468, 797
LweI GCATC 1 cut(s) 408
MaeI CTAG 1 cut(s) 690
MaeII ACGT 4 cut(s) 338, 387, 701, 720
MaeIII GTNAC 2 cut(s) 260, 274
MalI GATC 2 cut(s) 224, 570
MboI GATC 2 cut(s) 222, 568
MboII GAAGA 1 cut(s) 46
MflI RGATCY 2 cut(s) 222, 568
MhlI GDGCHC 4 cut(s) 232, 245, 497, 772
MluCI AATT 1 cut(s) 136
MluI ACGCGT 1 cut(s) 672
Mly113I GGCGCC 3 cut(s) 132, 143, 363
MlyI GAGTC 2 cut(s) 229, 760
MmeI TCCRAC 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 801
MspI CCGG 7 cut(s) 117, 129, 177, 187, 258, 312, 332
MspR9I CCNGG 5 cut(s) 117, 177, 187, 258, 499
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 5 cut(s) 167, 323, 433, 551, 674
MwoI GCNNNNNNNGC 7 cut(s) 140, 341, 369, 427, 511, 557, 666
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 3 cut(s) 132, 143, 363
NciI CCSGG 4 cut(s) 117, 177, 187, 258
NdeII GATC 2 cut(s) 222, 568
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 3 cut(s) 615, 650, 814
NlaIV GGNNCC 6 cut(s) 133, 144, 191, 364, 429, 470
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 2 cut(s) 615, 650
PaeI GCATGC 1 cut(s) 650
PasI CCCWGGG 1 cut(s) 498
PciI ACATGT 1 cut(s) 611
PcsI WCGNNNNNNNCGW 2 cut(s) 603, 678
PdiI GCCGGC 1 cut(s) 333
PdmI GAANNNNTTC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 185
PkrI GCNGC 6 cut(s) 101, 290, 320, 432, 458, 787
PleI GAGTC 2 cut(s) 229, 759
PluTI GGCGCC 3 cut(s) 135, 146, 366
PmaCI CACGTG 1 cut(s) 702
PmlI CACGTG 1 cut(s) 702
PpsI GAGTC 2 cut(s) 229, 759
Ppu21I YACGTR 1 cut(s) 702
PscI ACATGT 1 cut(s) 611
PshAI GACNNNNGTC 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 772
Psp6I CCWGG 1 cut(s) 497
PspCI CACGTG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 6 cut(s) 133, 144, 191, 364, 429, 470
PspPI GGNCC 3 cut(s) 8, 114, 189
PsuI RGATCY 2 cut(s) 222, 568
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 5 cut(s) 201, 407, 596, 629, 818
RsaNI GTAC 5 cut(s) 200, 406, 595, 628, 817
SacI GAGCTC 1 cut(s) 772
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 6 cut(s) 100, 289, 319, 431, 457, 786
Sau3AI GATC 2 cut(s) 222, 568
Sau96I GGNCC 3 cut(s) 8, 114, 189
SchI GAGTC 2 cut(s) 229, 760
ScrFI CCNGG 5 cut(s) 117, 177, 187, 258, 499
SduI GDGCHC 4 cut(s) 232, 245, 497, 772
SfaNI GCATC 1 cut(s) 408
SfoI GGCGCC 3 cut(s) 133, 144, 364
SinI GGWCC 2 cut(s) 8, 189
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SphI GCATGC 1 cut(s) 650
Sse9I AATT 1 cut(s) 136
SsiI CCGC 6 cut(s) 100, 288, 318, 329, 431, 711
SspDI GGCGCC 3 cut(s) 131, 142, 362
SspMI CTAG 1 cut(s) 690
SstI GAGCTC 1 cut(s) 772
StyD4I CCNGG 5 cut(s) 115, 175, 185, 256, 497
TaaI ACNGT 2 cut(s) 576, 621
TaiI ACGT 4 cut(s) 341, 390, 704, 723
TaqI TCGA 2 cut(s) 303, 772
TasI AATT 1 cut(s) 136
TatI WGTACW 1 cut(s) 816
TauI GCSGC 4 cut(s) 102, 291, 321, 433
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 2 cut(s) 163, 801
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 456, 785
Tsp45I GTSAC 1 cut(s) 274
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 2 cut(s) 163, 801
Tth111I GACNNNGTC 1 cut(s) 719
Van91I CCANNNNNTGG 1 cut(s) 210
VneI GTGCAC 1 cut(s) 241
VpaK11BI GGWCC 2 cut(s) 8, 189
XceI RCATGY 2 cut(s) 615, 650
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 1 cut(s) 690
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.