MD05G1124500.v1.1

Protein CHAPERONE-LIKE PROTEIN OF POR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009273
Physical Location & Seq
Reverse (-)
24664478 .. 24666527
2050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1124500.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCAGCCACTCTCTCCGTCCGGCCCAACCGCCTCTCCGCCGGCTCCTCCTTCCCTCGCCCGCCCCTCCGCCGCCCCGGACCCACTGGAATCCGAAAACCGGCCATGCAATTCGAGCCTTGGAGGGGCTCCATCGCCCCATCCCGCCGCACATTAACCTGGGCTGCCACGGCCGCCTCCAGAGCCGACGACAACGCACCGTTCGAGATGTCAGTCGAGAACGCCCTCAAGCTCCTCGGCGTCTCAGACGGCGCCTCGTTCGATGAAATTCTTCGCGCCAAGAACTCGATTGTCGCAGCTTGTAAAGATGACCAGGAGGCGATTGCACAGGTTGAGGCTGCGTACGACATGTTGCTTATGAGAAGCTTAACTCAGCGACGGGCAGGGAAAGTTGTGAATAGCAGTGTTCGTTATGCTGATGTTAAGCCAGTGAGTGCTCCTGGGATTGGATCAGTGCCGCAGTGGCTGCAGGCTACTGTGAAGAATCCACCTATTGCAGTGGAAACGCCCTCGACAAGTGATCTGGGTGTACAAGTTGGTGTCTATGGAGCTTTGATGGCCTTAACTTATGTCAATGGAGCTTCCGGTTCTTCTTCGGGGCCGTATGGAGGGGCTGATGTTCCTGGACTGATCCTGGCTGGTAGCTTTGGAGCTTCCTTGTACTTCATGACCAAAAAGAATATTAAGTTAGGGAAGGCGACCATCATAACTGTAGGGGGGCTTGTTGCTGGTGCCGTGGTGGGTTCAGCTGTAGAGAATTTTCTCCAAGTAGACATTGTTCCATTTCTTGGCATCCACTCTCCTGCTGCCGTAGTAAGCGAATTCATAATTTTTTTGCAATTAGTGGTCTCCCTATATTTACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CPP1-like

Protein Analysis

289

Amino Acids

30.19

Weight (kDa)

9.6

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CPP1-like PF11833 84 - 287 2.9e-55 Protein CHAPERONE-LIKE PROTEIN OF POR1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 251, 731
AccB7I CCANNNNNTGG 1 cut(s) 788
AccI GTMKAC 1 cut(s) 771
AccII CGCG 1 cut(s) 276
AciI CCGC 9 cut(s) 31, 39, 62, 70, 73, 145, 148, 174, 458
AclWI GGATC 2 cut(s) 457, 625
AcoI YGGCCR 2 cut(s) 102, 171
AcsI RAATTY 3 cut(s) 267, 757, 821
AcyI GRCGYC 2 cut(s) 240, 252
AfaI GTAC 3 cut(s) 344, 531, 662
AfiI CCNNNNNNNGG 5 cut(s) 100, 125, 446, 608, 788
AflIII ACRYGT 1 cut(s) 348
AjnI CCWGG 5 cut(s) 158, 312, 439, 622, 633
AluBI AGCT 8 cut(s) 232, 299, 366, 551, 581, 645, 653, 749
AluI AGCT 8 cut(s) 232, 299, 366, 551, 581, 645, 653, 749
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 247, 853
AlwI GGATC 2 cut(s) 457, 625
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 5 cut(s) 23, 102, 171, 558, 599
ApeKI GCWGC 6 cut(s) 5, 164, 296, 338, 466, 806
ApoI RAATTY 3 cut(s) 267, 757, 821
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 2 cut(s) 254, 278
AspS9I GGNCC 3 cut(s) 24, 80, 599
AsuC2I CCSGG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 80
BanI GGYRCC 2 cut(s) 251, 731
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 6 cut(s) 17, 151, 308, 325, 453, 793
BccI CCATC 4 cut(s) 140, 148, 550, 710
BceAI ACGGC 5 cut(s) 186, 265, 586, 719, 794
BciT130I CCWGG 5 cut(s) 160, 314, 441, 624, 635
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 247, 853
BfmI CTRYAG 3 cut(s) 467, 711, 750
BfoI RGCGCY 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 463
Bme1390I CCNGG 6 cut(s) 78, 160, 314, 441, 624, 635
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 3 cut(s) 24, 80, 599
BmiI GGNNCC 6 cut(s) 46, 82, 130, 253, 600, 733
BmrFI CCNGG 6 cut(s) 78, 160, 314, 441, 624, 635
BmsI GCATC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 78
BsaHI GRCGYC 2 cut(s) 240, 252
BsaI GGTCTC 1 cut(s) 853
BsaJI CCNNGG 7 cut(s) 76, 119, 159, 168, 235, 440, 735
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 125, 446, 608, 788
Bse118I RCCGGY 2 cut(s) 41, 100
Bse1I ACTGG 2 cut(s) 91, 428
BseBI CCWGG 5 cut(s) 160, 314, 441, 624, 635
BseDI CCNNGG 7 cut(s) 76, 119, 159, 168, 235, 440, 735
BseGI GGATG 2 cut(s) 140, 792
BseLI CCNNNNNNNGG 5 cut(s) 100, 125, 446, 608, 788
BseMII CTCAG 2 cut(s) 258, 386
BseNI ACTGG 2 cut(s) 91, 428
BseRI GAGGAG 2 cut(s) 37, 224
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 6 cut(s) 17, 151, 308, 325, 453, 793
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 5 cut(s) 25, 104, 173, 560, 601
BshNI GGYRCC 2 cut(s) 251, 731
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 5 cut(s) 22, 42, 78, 101, 585
BsiWI CGTACG 1 cut(s) 342
BslI CCNNNNNNNGG 5 cut(s) 100, 125, 446, 608, 788
BsmAI GTCTC 2 cut(s) 247, 853
BsmBI CGTCTC 1 cut(s) 247
BsnI GGCC 5 cut(s) 25, 104, 173, 560, 601
Bso31I GGTCTC 1 cut(s) 853
Bsp1286I GDGCHC 2 cut(s) 131, 439
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 449, 520, 630
BspACI CCGC 9 cut(s) 31, 39, 62, 70, 73, 145, 148, 174, 458
BspANI GGCC 5 cut(s) 25, 104, 173, 560, 601
BspCNI CTCAG 2 cut(s) 257, 385
BspFNI CGCG 1 cut(s) 276
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 6 cut(s) 46, 82, 130, 253, 600, 733
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 2 cut(s) 457, 625
BspT107I GGYRCC 2 cut(s) 251, 731
BspTNI GGTCTC 1 cut(s) 853
BsrFI RCCGGY 2 cut(s) 41, 100
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 2 cut(s) 91, 428
BssAI RCCGGY 2 cut(s) 41, 100
BssECI CCNNGG 7 cut(s) 76, 119, 159, 168, 235, 440, 735
BssMI GATC 3 cut(s) 449, 520, 630
BssNI GRCGYC 2 cut(s) 240, 252
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 5 cut(s) 160, 314, 441, 624, 635
Bst4CI ACNGT 4 cut(s) 201, 478, 712, 864
BstACI GRCGYC 2 cut(s) 240, 252
BstAPI GCANNNNNTGC 1 cut(s) 466
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 43, 62, 471
BstDEI CTNAG 2 cut(s) 244, 372
BstDSI CCRYGG 2 cut(s) 168, 735
BstF5I GGATG 2 cut(s) 140, 792
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 254, 278
BstKTI GATC 3 cut(s) 452, 523, 633
BstMAI GTCTC 2 cut(s) 247, 853
BstMBI GATC 3 cut(s) 449, 520, 630
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 7 cut(s) 115, 170, 173, 182, 463, 466, 557
BstNI CCWGG 5 cut(s) 160, 314, 441, 624, 635
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 6 cut(s) 76, 158, 312, 439, 622, 633
BstSFI CTRYAG 3 cut(s) 467, 711, 750
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 6 cut(s) 17, 151, 308, 325, 453, 793
BstZI CGGCCG 1 cut(s) 171
BsuRI GGCC 5 cut(s) 25, 104, 173, 560, 601
BtgI CCRYGG 2 cut(s) 168, 735
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 140, 792
BtsI GCAGTG 3 cut(s) 409, 467, 504
BtsIMutI CAGTG 6 cut(s) 84, 409, 435, 459, 467, 504
Cac8I GCNNGC 3 cut(s) 43, 62, 471
CaiI CAGNNNCTG 1 cut(s) 466
CciI TCATGA 1 cut(s) 666
CfoI GCGC 2 cut(s) 254, 278
Cfr10I RCCGGY 2 cut(s) 41, 100
Cfr13I GGNCC 3 cut(s) 24, 80, 599
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 343, 530, 661
CviAII CATG 3 cut(s) 106, 349, 667
CviQI GTAC 3 cut(s) 343, 530, 661
DdeI CTNAG 2 cut(s) 244, 372
DinI GGCGCC 1 cut(s) 253
DpnI GATC 3 cut(s) 451, 522, 632
DpnII GATC 3 cut(s) 449, 520, 630
EaeI YGGCCR 2 cut(s) 102, 171
EagI CGGCCG 1 cut(s) 171
EciI GGCGGA 2 cut(s) 28, 59
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 853
Eco47I GGWCC 1 cut(s) 80
Eco52I CGGCCG 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 5 cut(s) 158, 312, 439, 622, 633
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 119
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 109, 352, 670
FatI CATG 3 cut(s) 105, 348, 666
FauI CCCGC 2 cut(s) 69, 152
FblI GTMKAC 1 cut(s) 771
FokI GGATG 2 cut(s) 127, 779
FriOI GRGCYC 1 cut(s) 131
GlaI GCGC 2 cut(s) 253, 277
GsuI CTGGAG 1 cut(s) 163
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 5 cut(s) 25, 104, 173, 560, 601
HapII CCGG 5 cut(s) 22, 42, 78, 101, 585
HgaI GACGC 1 cut(s) 229
HhaI GCGC 2 cut(s) 254, 278
Hin1I GRCGYC 2 cut(s) 240, 252
Hin1II CATG 3 cut(s) 109, 352, 670
Hin6I GCGC 2 cut(s) 252, 276
HinP1I GCGC 2 cut(s) 252, 276
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 2 cut(s) 90, 484
HpaII CCGG 5 cut(s) 22, 42, 78, 101, 585
Hpy166II GTNNAC 2 cut(s) 530, 772
Hpy188I TCNGA 2 cut(s) 95, 247
Hpy188III TCNNGA 4 cut(s) 180, 205, 217, 667
Hpy8I GTNNAC 2 cut(s) 530, 772
Hpy99I CGWCG 2 cut(s) 191, 381
HpyAV CCTTC 2 cut(s) 61, 688
HpyCH4III ACNGT 4 cut(s) 201, 478, 712, 864
HpyCH4V TGCA 5 cut(s) 109, 326, 469, 497, 838
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 170, 173, 182, 463, 466, 557
HpyF3I CTNAG 2 cut(s) 244, 372
Hsp92I GRCGYC 2 cut(s) 240, 252
Hsp92II CATG 3 cut(s) 109, 352, 670
HspAI GCGC 2 cut(s) 252, 276
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Kzo9I GATC 3 cut(s) 449, 520, 630
LmnI GCTCC 7 cut(s) 50, 134, 237, 442, 548, 578, 650
Lsp1109I GCAGC 6 cut(s) 17, 151, 308, 325, 453, 793
LweI GCATC 1 cut(s) 801
MalI GATC 3 cut(s) 451, 522, 632
MboI GATC 3 cut(s) 449, 520, 630
MboII GAAGA 4 cut(s) 263, 493, 582, 585
MhlI GDGCHC 2 cut(s) 131, 439
MluCI AATT 6 cut(s) 110, 267, 757, 821, 828, 839
Mly113I GGCGCC 1 cut(s) 252
MroNI GCCGGC 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 5 cut(s) 155, 368, 423, 563, 684
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 5 cut(s) 22, 42, 78, 101, 585
MspR9I CCNGG 6 cut(s) 78, 160, 314, 441, 624, 635
MvaI CCWGG 5 cut(s) 160, 314, 441, 624, 635
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 7 cut(s) 115, 170, 173, 182, 463, 466, 557
NaeI GCCGGC 1 cut(s) 43
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 78
NdeII GATC 3 cut(s) 449, 520, 630
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 3 cut(s) 109, 352, 670
NlaIV GGNNCC 6 cut(s) 46, 82, 130, 253, 600, 733
NmeAIII GCCGAG 1 cut(s) 216
NspI RCATGY 1 cut(s) 352
PagI TCATGA 1 cut(s) 666
PciI ACATGT 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 90, 484
Pfl23II CGTACG 1 cut(s) 342
PflMI CCANNNNNTGG 1 cut(s) 788
PfoI TCCNGGA 1 cut(s) 622
PluTI GGCGCC 1 cut(s) 255
PscI ACATGT 1 cut(s) 348
Psp6I CCWGG 5 cut(s) 158, 312, 439, 622, 633
PspGI CCWGG 5 cut(s) 158, 312, 439, 622, 633
PspLI CGTACG 1 cut(s) 342
PspN4I GGNNCC 6 cut(s) 46, 82, 130, 253, 600, 733
PspPI GGNCC 3 cut(s) 24, 80, 599
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PstI CTGCAG 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 3 cut(s) 344, 531, 662
RsaNI GTAC 3 cut(s) 343, 530, 661
SaqAI TTAA 5 cut(s) 155, 368, 423, 563, 684
Sau3AI GATC 3 cut(s) 449, 520, 630
Sau96I GGNCC 3 cut(s) 24, 80, 599
ScrFI CCNGG 6 cut(s) 78, 160, 314, 441, 624, 635
SduI GDGCHC 2 cut(s) 131, 439
SfaNI GCATC 1 cut(s) 801
SfcI CTRYAG 3 cut(s) 467, 711, 750
SfoI GGCGCC 1 cut(s) 253
SinI GGWCC 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 6 cut(s) 110, 267, 757, 821, 828, 839
SsiI CCGC 9 cut(s) 31, 39, 62, 70, 73, 145, 148, 174, 458
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 1 cut(s) 682
StyD4I CCNGG 6 cut(s) 76, 158, 312, 439, 622, 633
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 4 cut(s) 201, 478, 712, 864
TaqI TCGA 6 cut(s) 114, 204, 216, 261, 287, 512
TasI AATT 6 cut(s) 110, 267, 757, 821, 828, 839
TatI WGTACW 2 cut(s) 529, 660
TauI GCSGC 4 cut(s) 75, 150, 176, 460
TfiI GAWTC 2 cut(s) 90, 484
Tru1I TTAA 5 cut(s) 155, 368, 423, 563, 684
Tru9I TTAA 5 cut(s) 155, 368, 423, 563, 684
TscAI CASTG 6 cut(s) 91, 409, 435, 459, 467, 504
TseI GCWGC 6 cut(s) 5, 164, 296, 338, 466, 806
TspDTI ATGAA 3 cut(s) 279, 655, 814
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 6 cut(s) 91, 409, 435, 459, 467, 504
Van91I CCANNNNNTGG 1 cut(s) 788
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 3 cut(s) 267, 757, 821
XceI RCATGY 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.