MD10G1127300.v1.1

Protein CHAPERONE-LIKE PROTEIN OF POR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009273
Physical Location & Seq
Reverse (-)
20748938 .. 20750986
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1127300.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCAGTCACTCTCTCCGTCCGGCCCCACCGCCTCTCCGCCGGCTCCTCCTTCCCCCGCCCGCCAGTCCGCCTCCCCGGCCCGGCCCGAATCCGAAAGCCCCCCATGCAATTTGTGCCTTGGAGGGGCTCCATCGTCCCCCCCCGCCGCACATTAACCTGGGCCGCCACGGCCGGCTCCAGGGCCGACGACAACGCGCCTTTCGAGATGTCGGTCGAGAACGCCCTCAAGCTTCTCGGCGTCTCAGACGGCGCGTCGTTCGATGAAATTCTTCGCGCCAAGAACTCGATTGTCGCGGCTTGTAAAGATGACCAGGAGGCGATTGCACAGGTTGAGGCGGCATACGACATGTTGCTTATGAGAAGCTTAACTCAGCGACGGGCGGGGAAAGTTGTGAATAGCAGTGTTCGTTATGCTGATGTAAAGCCAGTGAGTGCTTCTGGGATTGGATCAGTGCCTCAGTGGCTGCAGGCTACTGTGAAGAATCCACCCATTGCAGTGGAAATGCCCTCGACGAGTGATCTGGGTGTACAAGTTGGTGTTTATGGAGCTTTGATGGCCTTCACCTATGTCAATGGAGCTTCCGGTTCTTCTTCGGGGCCATATGGAGGGGCTGATATTCCTGGACTGATCCTGGCTGGTAGCTTTGGAGCTTCCTTGTACTTCATGACCAAAAAGAATATTAAGTTAGGGAAGGCGACTATCATAACAGTAGGGGGGCTTGTTGCTGGTGCCGTGGTGGGTTCAGCAGTAGAGAATTTTCTGCAAGTAGACATTGTTCCGTTTCTTGGTATTCACTCTCCTGTTGCCGTAGTAAGCGAATTTATAATTTTTTTGCAATTAGTGGTCTCCTTATATTTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CPP1-like

Protein Analysis

289

Amino Acids

30.34

Weight (kDa)

9.68

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CPP1-like PF11833 84 - 287 1.4e-53 Protein CHAPERONE-LIKE PROTEIN OF POR1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
AccB1I GGYRCC 1 cut(s) 731
AccI GTMKAC 1 cut(s) 771
AccII CGCG 4 cut(s) 197, 254, 276, 296
AclWI GGATC 2 cut(s) 457, 625
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 3 cut(s) 267, 757, 821
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 531, 662
AfiI CCNNNNNNNGG 5 cut(s) 82, 125, 180, 608, 788
AflIII ACRYGT 1 cut(s) 348
AhdI GACNNNNNGTC 1 cut(s) 253
AjnI CCWGG 5 cut(s) 158, 179, 312, 622, 633
AluBI AGCT 6 cut(s) 232, 366, 551, 581, 645, 653
AluI AGCT 6 cut(s) 232, 366, 551, 581, 645, 653
Alw26I GTCTC 2 cut(s) 247, 853
AlwI GGATC 2 cut(s) 457, 625
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 8 cut(s) 23, 79, 84, 162, 171, 183, 558, 599
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 3 cut(s) 267, 757, 821
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 3 cut(s) 199, 254, 278
AspS9I GGNCC 6 cut(s) 24, 80, 85, 162, 183, 599
AsuC2I CCSGG 2 cut(s) 78, 83
AsuHPI GGTGA 1 cut(s) 556
BanI GGYRCC 1 cut(s) 731
BanII GRGCYC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 140, 550
BceAI ACGGC 4 cut(s) 186, 265, 719, 794
BciT130I CCWGG 5 cut(s) 160, 181, 314, 624, 635
BcnI CCSGG 2 cut(s) 78, 83
BcoDI GTCTC 2 cut(s) 247, 853
BfmI CTRYAG 1 cut(s) 467
BglI GCCNNNNNGGC 3 cut(s) 78, 170, 463
BisI GCNGC 5 cut(s) 148, 165, 297, 339, 467
BlsI GCNGC 5 cut(s) 149, 166, 298, 340, 468
Bme1390I CCNGG 7 cut(s) 78, 83, 160, 181, 314, 624, 635
BmeRI GACNNNNNGTC 1 cut(s) 253
BmgT120I GGNCC 6 cut(s) 24, 80, 85, 162, 183, 599
BmiI GGNNCC 6 cut(s) 26, 46, 130, 178, 600, 733
BmrFI CCNGG 7 cut(s) 78, 83, 160, 181, 314, 624, 635
BpmI CTGGAG 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 2 cut(s) 78, 83
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 853
BsaJI CCNNGG 6 cut(s) 76, 119, 159, 168, 180, 735
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 125, 180, 608, 788
Bse118I RCCGGY 2 cut(s) 41, 173
Bse1I ACTGG 2 cut(s) 65, 428
Bse3DI GCAATG 1 cut(s) 492
BseBI CCWGG 5 cut(s) 160, 181, 314, 624, 635
BseDI CCNNGG 6 cut(s) 76, 119, 159, 168, 180, 735
BseLI CCNNNNNNNGG 5 cut(s) 82, 125, 180, 608, 788
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 3 cut(s) 258, 386, 473
BseNI ACTGG 2 cut(s) 65, 428
BseRI GAGGAG 1 cut(s) 37
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 453
Bsh1236I CGCG 4 cut(s) 197, 254, 276, 296
Bsh1285I CGRYCG 2 cut(s) 174, 216
BshFI GGCC 8 cut(s) 25, 81, 86, 164, 173, 185, 560, 601
BshNI GGYRCC 1 cut(s) 731
BsiEI CGRYCG 2 cut(s) 174, 216
BsiSI CCGG 6 cut(s) 22, 42, 78, 83, 174, 585
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 5 cut(s) 82, 125, 180, 608, 788
BsmAI GTCTC 2 cut(s) 247, 853
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 8 cut(s) 25, 81, 86, 164, 173, 185, 560, 601
Bso31I GGTCTC 1 cut(s) 853
Bsp1286I GDGCHC 1 cut(s) 131
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 449, 520, 630
BspANI GGCC 8 cut(s) 25, 81, 86, 164, 173, 185, 560, 601
BspCNI CTCAG 3 cut(s) 257, 385, 472
BspFNI CGCG 4 cut(s) 197, 254, 276, 296
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 6 cut(s) 26, 46, 130, 178, 600, 733
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 2 cut(s) 457, 625
BspT107I GGYRCC 1 cut(s) 731
BspTNI GGTCTC 1 cut(s) 853
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 2 cut(s) 41, 173
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 2 cut(s) 65, 428
BssAI RCCGGY 2 cut(s) 41, 173
BssECI CCNNGG 6 cut(s) 76, 119, 159, 168, 180, 735
BssMI GATC 3 cut(s) 449, 520, 630
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 5 cut(s) 160, 181, 314, 624, 635
Bst4CI ACNGT 2 cut(s) 478, 712
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 4 cut(s) 43, 62, 175, 471
BstDEI CTNAG 3 cut(s) 244, 372, 459
BstDSI CCRYGG 2 cut(s) 168, 735
BstFNI CGCG 4 cut(s) 197, 254, 276, 296
BstHHI GCGC 3 cut(s) 199, 254, 278
BstKTI GATC 3 cut(s) 452, 523, 633
BstMAI GTCTC 2 cut(s) 247, 853
BstMBI GATC 3 cut(s) 449, 520, 630
BstMCI CGRYCG 2 cut(s) 174, 216
BstMWI GCNNNNNNNGC 6 cut(s) 78, 106, 115, 170, 463, 557
BstNI CCWGG 5 cut(s) 160, 181, 314, 624, 635
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 7 cut(s) 76, 81, 158, 179, 312, 622, 633
BstSFI CTRYAG 1 cut(s) 467
BstUI CGCG 4 cut(s) 197, 254, 276, 296
BstV1I GCAGC 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 499
BstZI CGGCCG 1 cut(s) 171
BsuRI GGCC 8 cut(s) 25, 81, 86, 164, 173, 185, 560, 601
BtgI CCRYGG 2 cut(s) 168, 735
BtsI GCAGTG 2 cut(s) 409, 504
BtsIMutI CAGTG 5 cut(s) 409, 435, 459, 467, 504
Cac8I GCNNGC 4 cut(s) 43, 62, 175, 471
CaiI CAGNNNCTG 1 cut(s) 466
CciI TCATGA 1 cut(s) 666
CfoI GCGC 3 cut(s) 199, 254, 278
Cfr10I RCCGGY 2 cut(s) 41, 173
Cfr13I GGNCC 6 cut(s) 24, 80, 85, 162, 183, 599
CseI GACGC 2 cut(s) 229, 243
Csp6I GTAC 2 cut(s) 530, 661
CviAII CATG 3 cut(s) 106, 349, 667
CviQI GTAC 2 cut(s) 530, 661
DdeI CTNAG 3 cut(s) 244, 372, 459
DpnI GATC 3 cut(s) 451, 522, 632
DpnII GATC 3 cut(s) 449, 520, 630
DraI TTTAAA 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 171
EagI CGGCCG 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 253
EciI GGCGGA 2 cut(s) 28, 59
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 853
Eco52I CGGCCG 1 cut(s) 171
EcoRII CCWGG 5 cut(s) 158, 179, 312, 622, 633
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 119
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 109, 352, 670
FaqI GGGAC 1 cut(s) 122
FatI CATG 3 cut(s) 105, 348, 666
FauI CCCGC 4 cut(s) 65, 69, 152, 376
FauNDI CATATG 1 cut(s) 604
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 5 cut(s) 148, 165, 297, 339, 467
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 5 cut(s) 148, 165, 297, 339, 467
GlaI GCGC 3 cut(s) 198, 253, 277
GluI GCNGC 5 cut(s) 148, 165, 297, 339, 467
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 8 cut(s) 25, 81, 86, 164, 173, 185, 560, 601
HapII CCGG 6 cut(s) 22, 42, 78, 83, 174, 585
HgaI GACGC 2 cut(s) 229, 243
HhaI GCGC 3 cut(s) 199, 254, 278
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 3 cut(s) 109, 352, 670
Hin6I GCGC 3 cut(s) 197, 252, 276
HinP1I GCGC 3 cut(s) 197, 252, 276
HindIII AAGCTT 2 cut(s) 230, 364
HinfI GANTC 2 cut(s) 90, 484
HpaII CCGG 6 cut(s) 22, 42, 78, 83, 174, 585
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 2 cut(s) 530, 772
Hpy188I TCNGA 2 cut(s) 95, 247
Hpy188III TCNNGA 3 cut(s) 205, 217, 667
Hpy8I GTNNAC 2 cut(s) 530, 772
Hpy99I CGWCG 4 cut(s) 191, 259, 381, 517
HpyAV CCTTC 3 cut(s) 61, 571, 688
HpyCH4III ACNGT 2 cut(s) 478, 712
HpyCH4V TGCA 6 cut(s) 109, 326, 469, 497, 766, 838
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 106, 115, 170, 463, 557
HpyF3I CTNAG 3 cut(s) 244, 372, 459
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 3 cut(s) 109, 352, 670
HspAI GCGC 3 cut(s) 197, 252, 276
KroI GCCGGC 2 cut(s) 41, 173
KroNI GCCGGC 2 cut(s) 43, 175
Kzo9I GATC 3 cut(s) 449, 520, 630
LmnI GCTCC 6 cut(s) 50, 134, 182, 548, 578, 650
Lsp1109I GCAGC 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 7
MalI GATC 3 cut(s) 451, 522, 632
MboI GATC 3 cut(s) 449, 520, 630
MboII GAAGA 4 cut(s) 263, 493, 582, 585
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 6 cut(s) 110, 267, 757, 821, 828, 839
MroNI GCCGGC 2 cut(s) 41, 173
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 4 cut(s) 155, 368, 684, 860
MslI CAYNNNNRTG 1 cut(s) 497
MspI CCGG 6 cut(s) 22, 42, 78, 83, 174, 585
MspR9I CCNGG 7 cut(s) 78, 83, 160, 181, 314, 624, 635
MvaI CCWGG 5 cut(s) 160, 181, 314, 624, 635
MvnI CGCG 4 cut(s) 197, 254, 276, 296
MwoI GCNNNNNNNGC 6 cut(s) 78, 106, 115, 170, 463, 557
NaeI GCCGGC 2 cut(s) 43, 175
NciI CCSGG 2 cut(s) 78, 83
NdeI CATATG 1 cut(s) 604
NdeII GATC 3 cut(s) 449, 520, 630
NgoMIV GCCGGC 2 cut(s) 41, 173
NlaIII CATG 3 cut(s) 109, 352, 670
NlaIV GGNNCC 6 cut(s) 26, 46, 130, 178, 600, 733
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 352
PagI TCATGA 1 cut(s) 666
PciI ACATGT 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 2 cut(s) 43, 175
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 90, 484
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 5 cut(s) 149, 166, 298, 340, 468
PscI ACATGT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 827
Psp6I CCWGG 5 cut(s) 158, 179, 312, 622, 633
PspGI CCWGG 5 cut(s) 158, 179, 312, 622, 633
PspN4I GGNNCC 6 cut(s) 26, 46, 130, 178, 600, 733
PspPI GGNCC 6 cut(s) 24, 80, 85, 162, 183, 599
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PstI CTGCAG 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 466
RsaI GTAC 2 cut(s) 531, 662
RsaNI GTAC 2 cut(s) 530, 661
RseI CAYNNNNRTG 1 cut(s) 497
SaqAI TTAA 4 cut(s) 155, 368, 684, 860
SatI GCNGC 5 cut(s) 148, 165, 297, 339, 467
Sau3AI GATC 3 cut(s) 449, 520, 630
Sau96I GGNCC 6 cut(s) 24, 80, 85, 162, 183, 599
ScrFI CCNGG 7 cut(s) 78, 83, 160, 181, 314, 624, 635
SduI GDGCHC 1 cut(s) 131
SetI ASST 9 cut(s) 161, 234, 333, 368, 553, 569, 583, 647, 655
SfcI CTRYAG 1 cut(s) 467
SfiI GGCCNNNNNGGCC 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 6 cut(s) 110, 267, 757, 821, 828, 839
SspI AATATT 1 cut(s) 682
StyD4I CCNGG 7 cut(s) 76, 81, 158, 179, 312, 622, 633
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 2 cut(s) 478, 712
TaqI TCGA 5 cut(s) 204, 216, 261, 287, 512
TaqII GACCGA 1 cut(s) 202
TasI AATT 6 cut(s) 110, 267, 757, 821, 828, 839
TatI WGTACW 2 cut(s) 529, 660
TauI GCSGC 4 cut(s) 150, 167, 299, 341
TfiI GAWTC 2 cut(s) 90, 484
Tru1I TTAA 4 cut(s) 155, 368, 684, 860
Tru9I TTAA 4 cut(s) 155, 368, 684, 860
TscAI CASTG 5 cut(s) 409, 435, 459, 467, 504
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 279, 655
TspGWI ACGGA 2 cut(s) 7, 771
TspRI CASTG 5 cut(s) 409, 435, 459, 467, 504
XapI RAATTY 3 cut(s) 267, 757, 821
XceI RCATGY 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.