Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012052

Physical Location & Seq

Genomic Coordinates Reverse (-)

25207524 .. 25214786

Gene Structure 7263 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1128200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4563 bp

ATGGTGAAGGAAAGCACCAAAGCAAGCGGTCCAACCAAGAGGAAGCCGAAGGTCATATTTGGCACCAAAGCACTTCAGACGAGCGACGGCGATGGAGCTTCGAGCTTAGTCGGTGGCATAGTCGAGAAAGGTATTTCCGATATGCCGCTGTCGGGCCCCACTCCACCGCCTCGGCCGACGGTTCTTCCTTTCCCCGTCGCTCGCCACCGTTCCACCGGCCCTCATTGGGGTCCGGTGAATAGTAAATTGGGTGGTGAGGAGGATGGTGGTGAAGGGGATGATGAGGATGATGAAGATGTTATGGACTTCGAACACATCAGGGATTTCGCTATACGGGTTGAGAGGAAGAAGAAGAAGGATATGGACTTCAGTAAGTGGGCAGAGAAGGAGCTTGATGATAATAGCTCAAGAACTTTAAGGGAAACAATGGAATTTAGTACTAGAAAAATTGAGTCAAATAAATTGCTTTCCCGGCAAAAGAATGAACATGTATCTGCATTGGGTAACTTGAAGATTGAACAAGAATTTGTGTTGGGTAACTCGAAGAGCGAACAAGAATCTGTGTTGGGTAACTCGAAGAGTGAACAAGAATCTGTGTTTAACAACATGGAAATTGAAGCGGACATGAATGCAAATTCCATGCCTGATAATATTCGAAATGAACAAGGGGAATCTGTGTCACTTGAGGCTCAGATTGATGAAGAGAACCATGCACGGTTGCAAGGGATGTCTGCAGATGAGATTGAAGAAGCACAGGCGGAGATCATGGGGAGACTGGACCCTGCATTGCTCCAAGTACTCAAACGGAGGGGTGAAGAGAAATTGAGGAAGCAAAGAAGCCCCAGCTCAGATAACAATGAACCAAAAGCTTCCTCTAGCTCTCACAGTGGGCCGTCTCATGTGGCTACAAAAATTACTTCAAACGATACACAAACTTCAGTTAAGGATAGATTGGAGCAGAATTCAGGCAAAGCCAGTGGCAGTTTGTGGAATGCTTGGAGTGAGAGAGTCCAGGCAGTTAGGGACTTAAGGTTTTCCTTGGATGGAACTGTTATTATAAATGGCTTCCACCAGATATCTCAGAGTAGTAATTTATCCGAGCGTGACTACCTGCGTACTGAGGGGGATCCCGGTGCTGCTGGTTATACAATTAAAGAAGCAGTGTCTCTTACTAGGAGTGTGATTCCTGGACAGCGGACGCTGGCTTTGCATTTCCTTTCAACTGTGCTTGATAAGGCATTACAAAATATTCAAGCACAAGATCAGTTTATTGGAAAAGATACTAGTAAACTCGACAAATCTGCTGACTGGGAAGCCGTTTGGGCTTATGCACTTGGCCCAGAACCTGAGCTTATTTTATCTCTTAGGATATGTCTCGATGATAACCACAATAATGTAGTTCTGGCTTGTGCCAAAGTTCTTCACCGCATATTAAGCTGTGATGTGAATGAGAGCTTCTTTGACGTCTCAGAGAAAATAGCAACCCTGCATATGGATATATTTACTGCCCCTGTATTCAGAAGCAAACCAGAGATTGATGTTGGTTTCCTTCGTGGTGGATTTTGGAAGTACAATGCCAAGCCTTCTAATATATTTGCTCTTGATGAGGAAATTATTAATGATGAGACTGAAGGGAAACGTACCATTCAGGATGATGTTGTAGTTGCTGGACAAGATTTTGCGGCAGGTTTGGTGCGGATGGGAATCCTTCCACGGCTTCGCTATGTTTTGGAGTCAGACCCTATGGCGGCTTTGGAAGAATACACAATCTCTATACTTATTGCAATTGCAAGGCATTCCCCAAAATGTGCGGTTGCAATCATGAATTGTGAAAGGCTTCTTGAAACAATTATCAGCAGATTTATTGCAAAAGACACTGTAGATATCCAGCCTTCTAAAATAAAGTCTGTTAGACTTCTAAAGGTGTTGGCTCAATCTGATCGGAAGAACTGTGTTGCTTTTATAAAGAATGGGACTTTCCAAACTATGACATGGCATTTGTATCAATCTATTTCCTTCCTTGACAACTGGGTAAAATCTGGAAAGGAAAACTGTAAACTTTCTTCGGCTTTGAAGGTTGAACAACTGCGTTTTTGGAAGGTTTTTATTCAGCATGGCTATTGTGTGTCATACTTCTCAGATATTTTCCGTAACTTGTGCTTGTGGCTGAACGCACCTACTATTGAAAAACTTATTGAGAATGATGTACTTTGTGAATTTGCATCCATCTCTGCTGAGGGATACCTTGTTCTGGAGGCTTTGGCTAGAAGGCTCCCAAGTCTGTTCTCACAGATCCAGGTTGGTCCAATGGTTGATATAGCTCTAAAGTGGATAGTTTTGAAGAATGATCCCAGCATATGCAAGTTCTTTGAGAGAGAAAATGGAAGTCGTGGTGGTCTTACTGCCCAGGATTTATCAGTCACTTCCTTGTTATGGGTGTATTCAGCTGTAGTACACATGCTTTTCAGAGTGCTTGAAAGAGTGATACCAGATGATTCTGTCCACTCACTTGAAAGTGGTGGTCATGTGCCATGGCTTCCAGAGTTTGTTCCTAAAGTGGGACTTGAAATGATTAAAAATGGGTTTATGGGCCATTCAGATACTCTTTATGCAAAATATGGAATAGATCCCAAGGGAGATGGCTCTTTCATTGAGAAACTGTGCCATTTGAGAAATCTTGGTAACTGCGAAACATCATTAGCTTCTGTATGTTGCTTACAGGGGTTGGTGGGGATTATTGTTAGCATCGATAAATTGATAGTGTTAGCCAGGACAGGCGTCCAAACACCGCCCCAAAATTACACTTCATCAAGAGAAGAGAGAGTACTTAAGGATGGTTTACTTAAGGGGTCTTTGGTTGAATTGAGAAGTGTGCAAAATACTTTTATGAAGTTAGTTGCTTCTGAGTGGCCCCATGTGCAGTCCATTGAGATGTTTGGCAGGGGAGGACCTGCTCCCGGGGTAGGAGTTGGCTGGGGTGCCTCTGGTGGAGGATATTGGTCTGGTACTGTTTTGTTATCACAGGCAGATGCAAGATTTCTCGTTTACTTACTTGAAACTTGGACGCTTGTGTCCAATTTTAATAGCCCCACAGAAGAAGAAATGACTTTTACTATGCTGGCAATTAATTCATCTTTAGTAGTATGTGTAGGTGCTGGACCAACAGACAGGACTTATGTGACAAAGGCGTTAAACATTTTGCTTGATGTATCGGTTCTTAAGTATCTTGATCTCTGTATTCGACGTTTTCTTTCCTCAAATGGGGGAATGAAACTGTTTGATTGGGATTATAAAGAAGAGGATTACCTACTCTTTAGTAAAACATTAGCTTCTCATTTCAGCGACAGATGGTTATCTGTAAAGAAAAAGCTCAAAGATAGTGATGGCATGAAATCCTCTGATAGTAAATCACTGAAAAAGGGTAAAGCTTCCCTCAATACCATATACGAGGAATCGGACACACCACCTATGATCAGTCAAAATTGCACATCTTTAGTTGCAGAGTGGGCTCACCAGAGACTGCCACTACCCATTTCCTGGTTTCTCAGTCCAATCTCGACCCTTTGTGATAGCAAGCATGCTGGTCTTAAGAAGTTCTCCAATGTACAAGACCTCATGCAGGATCAAGGTGATTTTCTTGGAGTTGCCAAATCTGGGCTTTTCTTCCTTTTGGGTATTGAAGCACTGTCCAGTTTCTTACCAGTTGACGTCCCATCCCCAGTTAATAGTGTTTCGTTGGTTTGGAAATTGCATTCTCTATCTGTGATCTTACTCGTTGGAATGGGTGTGGTTGAGGAGGAGAAGAGTAGGGTTGTTTTTGAAGCTTTACAAGATCTCTATGGGAATCTTGTTCATCAGGCAAGGCCGTCTACCTTATTGCCAGAACCTAGAAACGAAAACAACCTGGACGTTCTGGCATTCCAATCAGAGGTTCACGAGAGTTACTCAGTATTTATTGAAACTCTTGTGGATCAGTTTTCTGCTGTATCTTATGGTGATTTGATATATGGCCGGCAAGTTGCAGTTTATCTACATCGTTGTGTGGAAGCTCCTGTGCGCCTTGCTGCCTGGAATACACTAACCAACTCTCGTGTTCTTGAACTTCTGCCACCTCTTGAGAAATGCTTTACTGATGCAGAGGGGTACCTTGAACCTGCTGAGGATAATCCGGGTATTTTGGAGGCGTACGTTAAGTCATGGACTTCTGATGCCCTCGATAGGGCTGCAAGTCGAGGATCACTTGCATATAAACTGGTTATCCACCACCTTTCGGCTTTCATTTTCAACTCATACACTGGTGACAAGCTATTGCTTAGGAATAAGCTTTCAAGGTCTCTTTTGCGAGACTTCTCTCTGAAGCAACAACATGAGGCTATGATGCTGAACCTTATTCAATATAACAAGCCATCTATACCGCATGAGACCAAGCACGAGGATGGAGTGCCTGTGGGGAATGACGTTGAGAAGAGGCTCGAGTTACTGAACGAAACTTGTGAATTAAATTCCTCACTTTTAGCTGCTGTGGAGAAGATAAAGTCATCCTTAAAGAACAATCTGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030154 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048869 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RPAP1_C RPAP1_N

Protein Analysis

1521

Amino Acids

169.15

Weight (kDa)

5.65

Isoelectric Point (pI)

39.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPAP1_N	PF08621	231 - 274	1.8e-17	RPAP1-like, N-terminal
RPAP1_C	PF08620	342 - 413	2e-15	RPAP1-like, C-terminal
TPR_RPAP1	PF25766	1299 - 1430	3.4e-16	RNA polymerase II-associated protein 1, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012052)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38440
fragaria_vesca	FvH4_2g15920 FvH4_2g15920
malus_domestica	MD05G1128200.v1.1 MD10G1131500.v1.1
prunus_persica	Prupe.8G172300_v2.0.a1
pyrus_communis	pycom05g12410 pycom10g11260
rosa_chinensis	RchiOBHm_Chr6g0279371
rosa_laevigata	RLG00000013150
rosa_multiflora	Rmu_sc0026652.1_g000002 Rmu_ssc0000349.1_g000001
rosa_roxburghii	Rroxscaffold_7G00188950
rosa_rugosa	Rorug06G0124200
rosa_samantha	Rh6AG232900 Rh6BG237300 Rh6CG239400 Rh6DG231100
rosa_wichuraiana	Rw6G020340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1058, 1964, 3291
AasI	GACNNNNNNGTC	1 cut(s)	3070
AatII	GACGTC	2 cut(s)	1467, 3711
Acc36I	ACCTGC	4 cut(s)	1119, 1676, 2959, 4162
Acc65I	GGTACC	1 cut(s)	4143
AccB1I	GGYRCC	3 cut(s)	62, 2978, 4143
AccB7I	CCANNNNNTGG	1 cut(s)	3537
AccI	GTMKAC	1 cut(s)	3869
AclWI	GGATC	8 cut(s)	1121, 1134, 2287, 2342, 2621, 3630, 3978, 4243
AcoI	YGGCCR	2 cut(s)	173, 4009
AcsI	RAATTY	6 cut(s)	431, 524, 634, 961, 2216, 4501
AcuI	CTGAAG	5 cut(s)	59, 352, 921, 1650, 4376
AcyI	GRCGYC	3 cut(s)	1464, 2778, 3708
AflII	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
AflIII	ACRYGT	1 cut(s)	487
AhlI	ACTAGT	1 cut(s)	1283
AjnI	CCWGG	8 cut(s)	1011, 1186, 2295, 2406, 2768, 3536, 3903, 4067
AjuI	GAANNNNNNNTTGG	4 cut(s)	230, 262, 3915, 3947
AloI	GAACNNNNNNTCC	2 cut(s)	2232, 2264
AlwI	GGATC	8 cut(s)	1121, 1134, 2287, 2342, 2621, 3630, 3978, 4243
AlwNI	CAGNNNCTG	2 cut(s)	1346, 3520
Ama87I	CYCGRG	2 cut(s)	2957, 4472
AoxI	GGCC	9 cut(s)	154, 173, 217, 890, 1336, 2590, 2909, 3863, 4009
ApaI	GGGCCC	1 cut(s)	158
ApeKI	GCWGC	4 cut(s)	1136, 4064, 4223, 4517
ApoI	RAATTY	6 cut(s)	431, 524, 634, 961, 2216, 4501
ArsI	GACNNNNNNTTYG	4 cut(s)	1189, 1221, 1981, 2013
AseI	ATTAAT	2 cut(s)	1617, 3126
Asp700I	GAANNNNTTC	1 cut(s)	1836
Asp718I	GGTACC	1 cut(s)	4143
AspLEI	GCGC	1 cut(s)	4059
AsuC2I	CCSGG	5 cut(s)	472, 1131, 2958, 2959, 4170
AsuII	TTCGAA	2 cut(s)	309, 655
AvaI	CYCGRG	2 cut(s)	2957, 4472
AvaII	GGWCC	6 cut(s)	29, 230, 778, 2303, 2948, 3158
BaeGI	GKGCMC	1 cut(s)	158
BamHI	GGATCC	1 cut(s)	1126
BanI	GGYRCC	3 cut(s)	62, 2978, 4143
BanII	GRGCYC	2 cut(s)	158, 3511
BarI	GAAGNNNNNNTAC	4 cut(s)	2047, 2079, 2808, 2840
BauI	CACGAG	3 cut(s)	3935, 4089, 4430
BbvCI	CCTCAGC	2 cut(s)	2235, 4158
BbvI	GCAGC	4 cut(s)	1123, 4051, 4210, 4504
BceAI	ACGGC	5 cut(s)	103, 877, 1301, 1730, 3850
BcgI	CGANNNNNNTGC	4 cut(s)	1283, 1317, 4205, 4239
BciT130I	CCWGG	8 cut(s)	1013, 1188, 2297, 2408, 2770, 3538, 3905, 4069
BciVI	GTATCC	1 cut(s)	2234
BclI	TGATCA	1 cut(s)	3471
BcnI	CCSGG	5 cut(s)	472, 1131, 2958, 2959, 4170
BcuI	ACTAGT	1 cut(s)	1283
BfaI	CTAG	6 cut(s)	441, 876, 1173, 1284, 2265, 3888
BfmI	CTRYAG	3 cut(s)	732, 1878, 2448
BfrI	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
BfuAI	ACCTGC	4 cut(s)	1119, 1676, 2959, 4162
BfuI	GTATCC	1 cut(s)	2234
BglI	GCCNNNNNGGC	1 cut(s)	1322
BglII	AGATCT	1 cut(s)	3832
BisI	GCNGC	7 cut(s)	146, 1137, 1683, 1749, 4065, 4224, 4518
BlsI	GCNGC	7 cut(s)	147, 1138, 1684, 1750, 4066, 4225, 4519
BmcAI	AGTACT	3 cut(s)	439, 798, 2826
Bme18I	GGWCC	6 cut(s)	29, 230, 778, 2303, 2948, 3158
BmeT110I	CYCGRG	2 cut(s)	2957, 4472
BmrI	ACTGGG	3 cut(s)	1318, 2038, 3713
BmsI	GCATC	6 cut(s)	2231, 2754, 3019, 4123, 4198, 4368
BmuI	ACTGGG	3 cut(s)	1318, 2038, 3713
BoxI	GACNNNNGTC	1 cut(s)	2777
BplI	GAGNNNNNCTC	2 cut(s)	3929, 3961
BpmI	CTGGAG	1 cut(s)	2273
Bpu10I	CCTNAGC	4 cut(s)	1347, 2235, 4158, 4313
Bpu14I	TTCGAA	2 cut(s)	309, 655
BpuEI	CTTGAG	3 cut(s)	391, 704, 4136
BpuMI	CCSGG	5 cut(s)	472, 1131, 2958, 2959, 4170
Bsa29I	ATCGAT	1 cut(s)	2748
BsaBI	GATNNNNATC	2 cut(s)	1703, 3352
BsaHI	GRCGYC	3 cut(s)	1464, 2778, 3708
BsaI	GGTCTC	2 cut(s)	4338, 4415
BsaJI	CCNNGG	8 cut(s)	170, 1038, 1712, 2406, 2531, 2631, 2957, 2958
BsaWI	WCCGGW	1 cut(s)	232
BsaXI	ACNNNNNCTCC	2 cut(s)	4431, 4461
Bse118I	RCCGGY	2 cut(s)	215, 4011
Bse1I	ACTGG	9 cut(s)	780, 975, 1313, 2033, 3690, 3701, 3719, 4257, 4300
Bse3DI	GCAATG	1 cut(s)	785
Bse8I	GATNNNNATC	2 cut(s)	1703, 3352
BseBI	CCWGG	8 cut(s)	1013, 1188, 2297, 2408, 2770, 3538, 3905, 4069
BseCI	ATCGAT	1 cut(s)	2748
BseDI	CCNNGG	8 cut(s)	170, 1038, 1712, 2406, 2531, 2631, 2957, 2958
BseJI	GATNNNNATC	2 cut(s)	1703, 3352
BseMI	GCAATG	1 cut(s)	785
BseNI	ACTGG	9 cut(s)	780, 975, 1313, 2033, 3690, 3701, 3719, 4257, 4300
BseRI	GAGGAG	3 cut(s)	272, 3809, 3812
BseSI	GKGCMC	1 cut(s)	158
BseX3I	CGGCCG	1 cut(s)	173
BseXI	GCAGC	4 cut(s)	1123, 4051, 4210, 4504
BseYI	CCCAGC	3 cut(s)	842, 2351, 2973
BsgI	GTGCAG	1 cut(s)	2939
Bsh1285I	CGRYCG	1 cut(s)	176
BshFI	GGCC	9 cut(s)	156, 175, 219, 892, 1338, 2592, 2911, 3865, 4011
BshNI	GGYRCC	3 cut(s)	62, 2978, 4143
BshVI	ATCGAT	1 cut(s)	2748
BsiEI	CGRYCG	1 cut(s)	176
BsiHKCI	CYCGRG	2 cut(s)	2957, 4472
BsiSI	CCGG	7 cut(s)	216, 233, 472, 1131, 2958, 4012, 4169
BsiWI	CGTACG	1 cut(s)	4185
BslFI	GGGAC	4 cut(s)	1037, 1987, 2574, 3695
BsmBI	CGTCTC	2 cut(s)	900, 1471
BsmFI	GGGAC	4 cut(s)	1037, 1987, 2574, 3695
BsmI	GAATGC	5 cut(s)	634, 997, 1795, 3751, 3917
BsnI	GGCC	9 cut(s)	156, 175, 219, 892, 1338, 2592, 2911, 3865, 4011
Bso31I	GGTCTC	2 cut(s)	4338, 4415
BsoBI	CYCGRG	2 cut(s)	2957, 4472
Bsp119I	TTCGAA	2 cut(s)	309, 655
Bsp120I	GGGCCC	1 cut(s)	154
Bsp1286I	GDGCHC	2 cut(s)	158, 3511
Bsp1407I	TGTACA	1 cut(s)	3604
Bsp19I	CCATGG	1 cut(s)	2531
BspANI	GGCC	9 cut(s)	156, 175, 219, 892, 1338, 2592, 2911, 3865, 4011
BspDI	ATCGAT	1 cut(s)	2748
BspHI	TCATGA	1 cut(s)	1821
BspMAI	CTGCAG	1 cut(s)	736
BspMI	ACCTGC	4 cut(s)	1119, 1676, 2959, 4162
BspPI	GGATC	8 cut(s)	1121, 1134, 2287, 2342, 2621, 3630, 3978, 4243
BspQI	GCTCTTC	1 cut(s)	539
BspT104I	TTCGAA	2 cut(s)	309, 655
BspT107I	GGYRCC	3 cut(s)	62, 2978, 4143
BspTI	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
BspTNI	GGTCTC	2 cut(s)	4338, 4415
BsrDI	GCAATG	1 cut(s)	785
BsrFI	RCCGGY	2 cut(s)	215, 4011
BsrGI	TGTACA	1 cut(s)	3604
BsrI	ACTGG	9 cut(s)	780, 975, 1313, 2033, 3690, 3701, 3719, 4257, 4300
BssAI	RCCGGY	2 cut(s)	215, 4011
BssECI	CCNNGG	8 cut(s)	170, 1038, 1712, 2406, 2531, 2631, 2957, 2958
BssNI	GRCGYC	3 cut(s)	1464, 2778, 3708
BssSI	CACGAG	3 cut(s)	3935, 4089, 4430
BssT1I	CCWWGG	3 cut(s)	1038, 2531, 2631
Bst2BI	CACGAG	3 cut(s)	3935, 4089, 4430
Bst2UI	CCWGG	8 cut(s)	1013, 1188, 2297, 2408, 2770, 3538, 3905, 4069
Bst6I	CTCTTC	8 cut(s)	539, 572, 696, 810, 2811, 3291, 3797, 4460
BstACI	GRCGYC	3 cut(s)	1464, 2778, 3708
BstAFI	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
BstAUI	TGTACA	1 cut(s)	3604
BstBI	TTCGAA	2 cut(s)	309, 655
BstC8I	GCNNGC	7 cut(s)	25, 202, 1203, 3120, 3575, 3579, 4013
BstDSI	CCRYGG	2 cut(s)	1712, 2531
BstENI	CCTNNNNNAGG	1 cut(s)	3617
BstHHI	GCGC	1 cut(s)	4059
BstMCI	CGRYCG	1 cut(s)	176
BstMWI	GCNNNNNNNGC	6 cut(s)	472, 1207, 1322, 1434, 2917, 3506
BstNI	CCWGG	8 cut(s)	1013, 1188, 2297, 2408, 2770, 3538, 3905, 4069
BstNSI	RCATGY	3 cut(s)	491, 2461, 3581
BstPAI	GACNNNNGTC	1 cut(s)	2777
BstSFI	CTRYAG	3 cut(s)	732, 1878, 2448
BstSLI	GKGCMC	1 cut(s)	158
BstV1I	GCAGC	4 cut(s)	1123, 4051, 4210, 4504
BstX2I	RGATCY	4 cut(s)	1126, 2292, 2626, 3832
BstYI	RGATCY	4 cut(s)	1126, 2292, 2626, 3832
BstZI	CGGCCG	1 cut(s)	173
Bsu15I	ATCGAT	1 cut(s)	2748
BsuI	GTATCC	1 cut(s)	2234
BsuRI	GGCC	9 cut(s)	156, 175, 219, 892, 1338, 2592, 2911, 3865, 4011
BsuTUI	ATCGAT	1 cut(s)	2748
BtgI	CCRYGG	2 cut(s)	1712, 2531
BtgZI	GCGATG	1 cut(s)	105
BtsI	GCAGTG	1 cut(s)	1167
BtsIMutI	CAGTG	7 cut(s)	892, 982, 1167, 1875, 3410, 3683, 4293
BveI	ACCTGC	4 cut(s)	1119, 1676, 2959, 4162
Cac8I	GCNNGC	7 cut(s)	25, 202, 1203, 3120, 3575, 3579, 4013
CaiI	CAGNNNCTG	2 cut(s)	1346, 3520
CciI	TCATGA	1 cut(s)	1821
CfoI	GCGC	1 cut(s)	4059
Cfr10I	RCCGGY	2 cut(s)	215, 4011
Cfr9I	CCCGGG	1 cut(s)	2957
ClaI	ATCGAT	1 cut(s)	2748
CseI	GACGC	3 cut(s)	1207, 2767, 3073
CspCI	CAANNNNNGTGG	2 cut(s)	958, 993
DrdI	GACNNNNNNGTC	1 cut(s)	3070
DseDI	GACNNNNNNGTC	1 cut(s)	3070
EaeI	YGGCCR	2 cut(s)	173, 4009
EagI	CGGCCG	1 cut(s)	173
Eam1104I	CTCTTC	8 cut(s)	539, 572, 696, 810, 2811, 3291, 3797, 4460
EarI	CTCTTC	8 cut(s)	539, 572, 696, 810, 2811, 3291, 3797, 4460
EciI	GGCGGA	1 cut(s)	773
EclXI	CGGCCG	1 cut(s)	173
Eco130I	CCWWGG	3 cut(s)	1038, 2531, 2631
Eco24I	GRGCYC	2 cut(s)	158, 3511
Eco31I	GGTCTC	2 cut(s)	4338, 4415
Eco32I	GATATC	2 cut(s)	1077, 1885
Eco47I	GGWCC	6 cut(s)	29, 230, 778, 2303, 2948, 3158
Eco52I	CGGCCG	1 cut(s)	173
Eco57I	CTGAAG	5 cut(s)	59, 352, 921, 1650, 4376
Eco88I	CYCGRG	2 cut(s)	2957, 4472
EcoNI	CCTNNNNNAGG	1 cut(s)	3617
EcoO109I	RGGNCCY	2 cut(s)	155, 2948
EcoRI	GAATTC	1 cut(s)	961
EcoRII	CCWGG	8 cut(s)	1011, 1186, 2295, 2406, 2768, 3536, 3903, 4067
EcoRV	GATATC	2 cut(s)	1077, 1885
EcoT14I	CCWWGG	3 cut(s)	1038, 2531, 2631
EcoT38I	GRGCYC	2 cut(s)	158, 3511
ErhI	CCWWGG	3 cut(s)	1038, 2531, 2631
Esp3I	CGTCTC	2 cut(s)	900, 1471
FalI	AAGNNNNNCTT	4 cut(s)	2547, 2579, 4526, 4558
FaqI	GGGAC	4 cut(s)	1037, 1987, 2574, 3695
FauNDI	CATATG	2 cut(s)	1491, 2357
FbaI	TGATCA	1 cut(s)	3471
FblI	GTMKAC	1 cut(s)	3869
Fnu4HI	GCNGC	7 cut(s)	146, 1137, 1683, 1749, 4065, 4224, 4518
FriOI	GRGCYC	2 cut(s)	158, 3511
Fsp4HI	GCNGC	7 cut(s)	146, 1137, 1683, 1749, 4065, 4224, 4518
FspBI	CTAG	6 cut(s)	441, 876, 1173, 1284, 2265, 3888
GlaI	GCGC	1 cut(s)	4058
GluI	GCNGC	7 cut(s)	146, 1137, 1683, 1749, 4065, 4224, 4518
GsaI	CCCAGC	3 cut(s)	846, 2355, 2977
GsuI	CTGGAG	1 cut(s)	2273
HaeIII	GGCC	9 cut(s)	156, 175, 219, 892, 1338, 2592, 2911, 3865, 4011
HapII	CCGG	7 cut(s)	216, 233, 472, 1131, 2958, 4012, 4169
HgaI	GACGC	3 cut(s)	1207, 2767, 3073
HhaI	GCGC	1 cut(s)	4059
Hin1I	GRCGYC	3 cut(s)	1464, 2778, 3708
Hin6I	GCGC	1 cut(s)	4057
HinP1I	GCGC	1 cut(s)	4057
HincII	GTYRAC	1 cut(s)	3706
HindII	GTYRAC	1 cut(s)	3706
HindIII	AAGCTT	4 cut(s)	867, 3426, 3822, 4322
HpaII	CCGG	7 cut(s)	216, 233, 472, 1131, 2958, 4012, 4169
Hpy99I	CGWCG	4 cut(s)	89, 181, 200, 3246
HpyCH4IV	ACGT	7 cut(s)	1464, 1639, 3244, 3708, 3909, 4188, 4458
HpyF10VI	GCNNNNNNNGC	6 cut(s)	472, 1207, 1322, 1434, 2917, 3506
HpySE526I	ACGT	7 cut(s)	1464, 1639, 3244, 3708, 3909, 4188, 4458
Hsp92I	GRCGYC	3 cut(s)	1464, 2778, 3708
HspAI	GCGC	1 cut(s)	4057
KpnI	GGTACC	1 cut(s)	4147
KroI	GCCGGC	1 cut(s)	4011
KroNI	GCCGGC	1 cut(s)	4013
Ksp22I	TGATCA	1 cut(s)	3471
LguI	GCTCTTC	1 cut(s)	539
LmnI	GCTCC	7 cut(s)	95, 388, 795, 955, 2277, 2959, 4054
Lsp1109I	GCAGC	4 cut(s)	1123, 4051, 4210, 4504
LweI	GCATC	6 cut(s)	2231, 2754, 3019, 4123, 4198, 4368
MaeI	CTAG	6 cut(s)	441, 876, 1173, 1284, 2265, 3888
MaeII	ACGT	7 cut(s)	1464, 1639, 3244, 3708, 3909, 4188, 4458
MfeI	CAATTG	1 cut(s)	1785
MflI	RGATCY	4 cut(s)	1126, 2292, 2626, 3832
MhlI	GDGCHC	2 cut(s)	158, 3511
MlyI	GAGTC	3 cut(s)	461, 1017, 1742
MmeI	TCCRAC	2 cut(s)	56, 3757
MroNI	GCCGGC	1 cut(s)	4011
MroXI	GAANNNNTTC	1 cut(s)	1836
MslI	CAYNNNNRTG	4 cut(s)	1392, 2930, 4038, 4434
MspA1I	CMGCKG	3 cut(s)	148, 1195, 2447
MspCI	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
MspI	CCGG	7 cut(s)	216, 233, 472, 1131, 2958, 4012, 4169
MunI	CAATTG	1 cut(s)	1785
Mva1269I	GAATGC	5 cut(s)	634, 997, 1795, 3751, 3917
MvaI	CCWGG	8 cut(s)	1013, 1188, 2297, 2408, 2770, 3538, 3905, 4069
MwoI	GCNNNNNNNGC	6 cut(s)	472, 1207, 1322, 1434, 2917, 3506
NaeI	GCCGGC	1 cut(s)	4013
NciI	CCSGG	5 cut(s)	472, 1131, 2958, 2959, 4170
NcoI	CCATGG	1 cut(s)	2531
NdeI	CATATG	2 cut(s)	1491, 2357
NgoMIV	GCCGGC	1 cut(s)	4011
NmeAIII	GCCGAG	1 cut(s)	151
NmuCI	GTSAC	5 cut(s)	678, 1103, 2419, 3178, 4298
NspI	RCATGY	3 cut(s)	491, 2461, 3581
NspV	TTCGAA	2 cut(s)	309, 655
PaeI	GCATGC	1 cut(s)	3581
PaeR7I	CTCGAG	1 cut(s)	4472
PagI	TCATGA	1 cut(s)	1821
PciI	ACATGT	1 cut(s)	487
PciSI	GCTCTTC	1 cut(s)	539
PctI	GAATGC	5 cut(s)	634, 997, 1795, 3751, 3917
PdiI	GCCGGC	1 cut(s)	4013
PdmI	GAANNNNTTC	1 cut(s)	1836
PfeI	GAWTC	8 cut(s)	557, 590, 671, 1183, 1704, 2495, 3452, 3844
Pfl23II	CGTACG	1 cut(s)	4185
PflMI	CCANNNNNTGG	1 cut(s)	3537
PfoI	TCCNGGA	1 cut(s)	1186
PkrI	GCNGC	7 cut(s)	147, 1138, 1684, 1750, 4066, 4225, 4519
PleI	GAGTC	3 cut(s)	460, 1016, 1741
PpsI	GAGTC	3 cut(s)	460, 1016, 1741
PpuMI	RGGWCCY	1 cut(s)	2948
PscI	ACATGT	1 cut(s)	487
PshAI	GACNNNNGTC	1 cut(s)	2777
PshBI	ATTAAT	2 cut(s)	1617, 3126
PsiI	TTATAA	3 cut(s)	1058, 1964, 3291
Psp5II	RGGWCCY	1 cut(s)	2948
Psp6I	CCWGG	8 cut(s)	1011, 1186, 2295, 2406, 2768, 3536, 3903, 4067
PspFI	CCCAGC	3 cut(s)	842, 2351, 2973
PspGI	CCWGG	8 cut(s)	1011, 1186, 2295, 2406, 2768, 3536, 3903, 4067
PspLI	CGTACG	1 cut(s)	4185
PspOMI	GGGCCC	1 cut(s)	154
PspPPI	RGGWCCY	1 cut(s)	2948
PspXI	VCTCGAGB	1 cut(s)	4472
PstI	CTGCAG	1 cut(s)	736
PstNI	CAGNNNCTG	2 cut(s)	1346, 3520
PsuI	RGATCY	4 cut(s)	1126, 2292, 2626, 3832
PvuII	CAGCTG	1 cut(s)	2447
RseI	CAYNNNNRTG	4 cut(s)	1392, 2930, 4038, 4434
SapI	GCTCTTC	1 cut(s)	539
SatI	GCNGC	7 cut(s)	146, 1137, 1683, 1749, 4065, 4224, 4518
ScaI	AGTACT	3 cut(s)	439, 798, 2826
SchI	GAGTC	3 cut(s)	461, 1017, 1742
SduI	GDGCHC	2 cut(s)	158, 3511
SfaNI	GCATC	6 cut(s)	2231, 2754, 3019, 4123, 4198, 4368
SfcI	CTRYAG	3 cut(s)	732, 1878, 2448
Sfr274I	CTCGAG	1 cut(s)	4472
SfuI	TTCGAA	2 cut(s)	309, 655
SinI	GGWCC	6 cut(s)	29, 230, 778, 2303, 2948, 3158
SlaI	CTCGAG	1 cut(s)	4472
SmaI	CCCGGG	1 cut(s)	2959
SmiMI	CAYNNNNRTG	4 cut(s)	1392, 2930, 4038, 4434
SmlI	CTYRAG	9 cut(s)	406, 683, 1027, 2828, 2843, 3218, 3587, 4115, 4472
SmoI	CTYRAG	9 cut(s)	406, 683, 1027, 2828, 2843, 3218, 3587, 4115, 4472
SpeI	ACTAGT	1 cut(s)	1283
SphI	GCATGC	1 cut(s)	3581
SspI	AATATT	2 cut(s)	652, 1249
SspMI	CTAG	6 cut(s)	441, 876, 1173, 1284, 2265, 3888
StyI	CCWWGG	3 cut(s)	1038, 2531, 2631
TaiI	ACGT	7 cut(s)	1467, 1642, 3247, 3711, 3912, 4191, 4461
TatI	WGTACW	7 cut(s)	437, 796, 1569, 2206, 2452, 2824, 3604
TauI	GCSGC	3 cut(s)	148, 1685, 1751
TfiI	GAWTC	8 cut(s)	557, 590, 671, 1183, 1704, 2495, 3452, 3844
TscAI	CASTG	7 cut(s)	892, 982, 1167, 1882, 3417, 3690, 4300
TseFI	GTSAC	5 cut(s)	678, 1103, 2419, 3178, 4298
TseI	GCWGC	4 cut(s)	1136, 4064, 4223, 4517
Tsp45I	GTSAC	5 cut(s)	678, 1103, 2419, 3178, 4298
TspGWI	ACGGA	2 cut(s)	820, 2138
TspMI	CCCGGG	1 cut(s)	2957
TspRI	CASTG	7 cut(s)	892, 982, 1167, 1882, 3417, 3690, 4300
Van91I	CCANNNNNTGG	1 cut(s)	3537
Vha464I	CTTAAG	5 cut(s)	1027, 2828, 2843, 3218, 3587
VpaK11BI	GGWCC	6 cut(s)	29, 230, 778, 2303, 2948, 3158
VspI	ATTAAT	2 cut(s)	1617, 3126
XagI	CCTNNNNNAGG	1 cut(s)	3617
XapI	RAATTY	6 cut(s)	431, 524, 634, 961, 2216, 4501
XceI	RCATGY	3 cut(s)	491, 2461, 3581
XhoI	CTCGAG	1 cut(s)	4472
XmaI	CCCGGG	1 cut(s)	2957
XmiI	GTMKAC	1 cut(s)	3869
XmnI	GAANNNNTTC	1 cut(s)	1836
XspI	CTAG	6 cut(s)	441, 876, 1173, 1284, 2265, 3888
ZraI	GACGTC	2 cut(s)	1465, 3709
ZrmI	AGTACT	3 cut(s)	439, 798, 2826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.