Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012052

Physical Location & Seq

Genomic Coordinates Reverse (-)

21181937 .. 21189112

Gene Structure 7176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1131500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4596 bp

ATGGCGGAGAAGCAAAGCACCAAAGCAAGCGGTCCAGGCCCGAGGAAGCCGAAGGTCATATTTGGCACCAAAGCACTTCAAACCGGGGACGGCGGCGATGTTCCTTCGAGCTTACTCGGCGGCATAGTCGAGAAAGGTATTTCCGATATGCCCCTGTCGGGCCCCACTCCACCGCCTCGCCCAACCGTTCTTCCTTTCCCCGTCGCTCGCCACCGCTCCGATGGCCCGCATCGGGGTCCGGTAAATAGTAAATTGGGTGGTGAGGAGGGTGGTGGGCAAGGGGATGAGGAGGATGATGAAGATGTAATGGACTTTGAACACATTAGGGATTTCGCTAAGCGGATCGAAAGGACGAAGAAAAAAGATATGGACTTTAGTAAGTGGGCAGAGGAGGAGCTTGATGCAAATAGCTCAATAACTTTTAGGGAAACAATGGAAGCTAGTACCAGAAAAATTGAGTCAAGTAAAGTGCATTCCCAGCGAAAGAATGAACAAGAATCTGCATTTGGTAGCTCGAAGATTGAACGAGGATCTGTGTTGGGTAACTTGAAGGTTGAACAAGAATCTGTTTTGGGTCACAATAACATGGAAATTGAAGCGGACATGAACGGAAAGTCCATGCCTGATAATATTCGAAATGAACAAGGGGGGTCTGTGTCGCTTGAGGCTCAGATTGATGAAGAGAACTGTGCACGGTTGCAAGGGATGTCTGCAGATGAGATTGAAGAAGCACAGGCGGAGATCATGGGCAGACTGGACCCTGCATTGCTCCAAGTACTCAAAAGGAGGGGTGAAGAGAAATTGAGAAAGCAAAGAGGCCCCAGCTCAGATAGCAGTGAACCAAAAGCTTCTACTATCTCTCACAGTGGTCCGTCTCATGTGGCTACAAAAATTACTTCAAACAATACTCGAACTTCAGCAAAGGATAGATTGGAGCAGAATTCAGGCAAAGCCAGTGGCAGTTTGTGGAATGCTTGGAGTGATAGAGTCCAGGCTGCTAGGGACTTAAGGTTTTCCTTGGATGGGACTGTTATTATAGATGGCTTCCACCAGATATCTCAGAGTAGTAATTTATCTGAGCGTGACTACCTGCGTACCGAGGGGGACCCTGGTGCTGCTGGTTATACAATCAAAGAAGCAGTGTCGCTCACTAGGAGTGTGATTCCTGGACAGCGGACACTTGCTTTGCATTTCCTTTCAACCGTGCTTGATAAGGCATTGCATAATATTCAAGCACAAGATCAGCTTATTGGAAAAGATGCTAGTAAAGTCGATAAATCTGCTGACTGGGAGGCCATTTGGGCTTATGCACTTGGCCCAGAACCTGAGCTTATTTTATCTCTCAGGATATGTCTCGATGATAACCACAATAATGTAGTCCTAGCTTGTGCCAAAGTTCTTCACCGCATATTAAGCTGTGATGTGAATGAGAACTTCTTTGACGTTTCAGAGAAAATAGCAACCTTGCATATCGACACATTTACTGCCCCTGTATTCAGAAGCAAACCAGAGATTGATGTTGGTTTCCTGCATGGTGGATTTTGGAAGTACAACACCAAGCCTTCTAATATTGTTTCTCTCGATGAGGAAATTATTGATGATGAAACTGAAGGGAAACGCACCATTCAAGATGATGTTGTAGTTGCTGGACAAGATTTTGCGGCAGGTTTGGTGCGGATGGGAATCCTTCCAAGGCTTCGCTATGTTTTGGAGTCTGACCCTACGGCAGCTTTGGAAGAATACACAATATCTATACTTATTGCAATTGCAAGGCATTCCCCAAAATGTGCGAATGCAATCATGAATTGTGAAAGGCTTCTTGAAACAATTGTCAGCAGATTTATCGGAGAAGACAGTGTAGATATCCAGCCTTCAAAAATAAAGTCTGTTAGACTTCTAAAGGTGATGGCTCAATCTGATCGTAAGAACTGTGTTGCTTTTATAAAGAATGGGACTTTCCAAACTATGACATGGCATTTGTATCAATCTATTTGCTTCCTTGACAACTGGGTAAAATCTGGAAAGGAAAACTGTAAACTTTCTTCGGCTTTGAAGGTTGAACAGCTGCGTTTTTGGAAGGTTTTTGTTCAGCATGGCTATTGTGTGTCATACTTCTCAGATATTTTCCATAACTTGTGCTTGTGGCTGAACCCACCTACAATTGAAAAACTTATTGAGAATGATGTACTTGGTGAATTTACGTCCATCTCTACAGAAGGATACCTTGTTCTGGAGGCTTTGGCTAGAAGGCTCCCAAGTTTGTTCTCACAGAAGCGTCTGAGCAATGAAATCTCTGAGCATTCTGGTGATGGAACTGAGTTCTGGTCTTGGAGCCAAGTTGGTCCAATGGTTGATATAGCTCTCAAGTGGATAGTTTTGATGAGTGATCCCAGTATTTGTAATTTCTTTGAGAGAGAAAGTGGAAGTCGTGGTGGTCTTGCTTCCCAAGATTTATCAGTCACTTCCTTGTTATGGGTGTATTCAGCTGTAGTACAAATGCTTTCCAGAGTGCTTGAAAGAGTGGTCCCAGATGATTCTGTCCACTCACATGAAAGTGGTAGTCTTGTGCCATGGCTTCCAGAGTTTGTTCCTAAAGTTGGACTTGAAATGATTAAAAATGGGTTTATAGGCTGTTCAGATACTCTTGATGCGAAATTTGGAAAAGATCCTAGCGGAGGTGACTCTTTCATTGAGAAACTGTCTCATTTGAGAAATCTGGGTAAATGCGAAACATCATTAGCTTCTGTAAGTTGCTTACAGGGGTTAGTGGGGCTTGTCGTTAGCATTGATAAGTTGATAATGTTAGCCAGGACAGGCGTCCAAACACCTCCCCAAAATTACCCTTCATCTAGAGAAGAGAAAATACTCAAGGACGGTATACTTAAGGGGTCTTTGGTTGAATTGAGAAGTGTGCAAAATACTTTTATGAAGTTAGTTGCTTCTGAGTGGCCCCTTGTGCAGTCCATTGAGATGTTTGGCAGGGGAGGACCTGCTCCCGGGGTGGGAGTTGGCTGGGGTGCCTCTGGTGGAGGATTTTGGTCTGGTAGTGTTTTGTTGTCGCAAGCAGATGCAAGATTTCTCGTTGACTTGCTTGAAACTTGGAAGCTTGTGTCCAATTTGGATAGCCCCACAGAAAAAGAAATGACTTTTACTATGCTGGCAATTAATTCATCTTTAGGAGTATGTGTAACTGCTGGACCAACAGACAGGACTTATGTGAGAAAGGTGTTAAATATTTTGCTTGATGTCTCAGTTCTTAAGTATCTTGATCTCTATATTCGACGTTTTCTTTCCTCAAATGGGGGTTTGAAACTGTTTGATTGGGATTATAAAGAAGAGGATTATGTACTCTTCAGTAAAACATTAGCTTCTCATTTCGGAGACAGATGGTTATCCATAAAGAAAAAGCTCAAAGATAGCGTGAATTCCTCTGATAGTAAATCACTGAAAAAGGGTAAAGGTTCCCTTGATACTATATACGAGGAATCGGACACATCACCTTTGATCACTCAAGATTGCACTTCTTTAGTTGTAGAGTGGGCTCACCAGAGACTGCCACTACCCATTTCCTGGTTTCTCAGTCCAATCTCAACCCTTTGTGATAGCAAGCATGCTGGTCTTAAGAAATTCTCCAATTTACAAGATCTCATGCAGGATCAAGGGGATTTTGTTGTAGTTGCCAAAGCTGGGCTTTTCTTTCTTTTGGGAATTGAAGCACTGTCCAGTTTCTTACCAGCTGACATTCCATCCCCAGTTAAAAGTGTATCGTTAGCTTGGAAATTGCATTCTCTATCTGTGATTTTACTCGTGGGAATGGGTGTGGTTGAGGAGGAGAAGAGTAGGGTTATTTTTGAAGCTTTACAAGATCTCTATGGGAACCTTCTTCATCAGTCAAGGTCGTCTGACTTAATGCCAGAACATAGAAATGAAAACAACCTGGAGGTTCTGGCATTCCAATCAGAGGTTCACGAGAGTTACTCTGTATTTATTGAAACTCTTGTGGATCAGTTTTCTGCTATATCTTATGGTGATTTGATATATGGCCGGCAAGTTGCAGTTTATCTGCATCGTTGTGTGGAAGCTCCTGTGCGCCTTGCTGCCTGGAATACGCTAACCACTTCTCGTGTTCTTGAACTTCTGCCACCTCTAGAGAAATGCTTTACTGATGCAGAGGGGTACCTTGAACCTGCAGAGGATAATCCGGATATTTTGGAGGTATACGTTAAGTCATGGACTTCTGGTGCCCTCGATAGGGCTGCAAGTCGAGGATCAGTTGCATATACACTGGTTATCCATCACCTTTCGGCTTTCATTTTTAGCTCATACACTGGTGATAAGCTATTGCTTAGGAATAAACTTTCAAGGTCTCTTTTGCGAGACTTCTCTCTGAAGCAGCAACATGAGGCGATGATGCTGAACCTTATTCAATATAACAAGGCATCTATATCGCATGAGACCAAGCACGAGGATGGAGTGCCTGTGGGGAATGACGTTGAGAAGAGGCTCGAGTTACTGAAGGAAACTTGTGAATTAAATTCCTCACTTTTAGCTGCCGTGGAGAAGCTAAAGTCATCCTTAAAGAACAATCTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030154 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048869 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RPAP1_C RPAP1_N

Protein Analysis

1532

Amino Acids

169.59

Weight (kDa)

5.56

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPAP1_N	PF08621	224 - 267	3.2e-17	RPAP1-like, N-terminal
RPAP1_C	PF08620	335 - 406	2.8e-15	RPAP1-like, C-terminal
TPR_RPAP1	PF25766	1307 - 1441	8.8e-17	RNA polymerase II-associated protein 1, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012052)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38440
fragaria_vesca	FvH4_2g15920 FvH4_2g15920
malus_domestica	MD05G1128200.v1.1 MD10G1131500.v1.1
prunus_persica	Prupe.8G172300_v2.0.a1
pyrus_communis	pycom05g12410 pycom10g11260
rosa_chinensis	RchiOBHm_Chr6g0279371
rosa_laevigata	RLG00000013150
rosa_multiflora	Rmu_sc0026652.1_g000002 Rmu_ssc0000349.1_g000001
rosa_roxburghii	Rroxscaffold_7G00188950
rosa_rugosa	Rorug06G0124200
rosa_samantha	Rh6AG232900 Rh6BG237300 Rh6CG239400 Rh6DG231100
rosa_wichuraiana	Rw6G020340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1943, 3330
Acc36I	ACCTGC	4 cut(s)	1098, 1655, 2998, 4195
Acc65I	GGTACC	1 cut(s)	4176
AccB1I	GGYRCC	4 cut(s)	65, 3017, 4176, 4241
AccB7I	CCANNNNNTGG	1 cut(s)	3570
AccBSI	CCGCTC	1 cut(s)	216
AccI	GTMKAC	2 cut(s)	2878, 4218
AccIII	TCCGGA	1 cut(s)	4201
AclWI	GGATC	7 cut(s)	350, 538, 2381, 2660, 3663, 4011, 4276
AcoI	YGGCCR	1 cut(s)	4042
AcsI	RAATTY	6 cut(s)	940, 2195, 2654, 3424, 3626, 4534
AcuI	CTGAAG	5 cut(s)	900, 1629, 3337, 4409, 4535
AcyI	GRCGYC	1 cut(s)	2817
AfaI	GTAC	8 cut(s)	445, 777, 1096, 1550, 2187, 2493, 3348, 4178
AflII	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
AjnI	CCWGG	8 cut(s)	34, 990, 1108, 1165, 2807, 3569, 3936, 4100
AjuI	GAANNNNNNNTTGG	4 cut(s)	489, 521, 3948, 3980
AloI	GAACNNNNNNTCC	4 cut(s)	599, 631, 2211, 2243
Alw21I	GWGCWC	1 cut(s)	694
Alw26I	GTCTC	9 cut(s)	879, 1358, 2706, 3253, 3375, 3544, 4371, 4371, 4448
Alw44I	GTGCAC	1 cut(s)	690
AlwI	GGATC	7 cut(s)	350, 538, 2381, 2660, 3663, 4011, 4276
AlwNI	CAGNNNCTG	2 cut(s)	1325, 3553
Ama87I	CYCGRG	3 cut(s)	40, 2996, 4505
Aor13HI	TCCGGA	1 cut(s)	4201
AoxI	GGCC	8 cut(s)	37, 160, 223, 817, 1293, 1315, 2948, 4042
ApaI	GGGCCC	1 cut(s)	164
ApaLI	GTGCAC	1 cut(s)	690
ApeKI	GCWGC	8 cut(s)	995, 1115, 1727, 2065, 4097, 4256, 4393, 4550
ApoI	RAATTY	6 cut(s)	940, 2195, 2654, 3424, 3626, 4534
ArsI	GACNNNNNNTTYG	6 cut(s)	1168, 1200, 1868, 1900, 1960, 1992
AseI	ATTAAT	1 cut(s)	3165
Asp700I	GAANNNNTTC	1 cut(s)	1815
Asp718I	GGTACC	1 cut(s)	4176
AspLEI	GCGC	1 cut(s)	4092
AsuC2I	CCSGG	3 cut(s)	85, 2997, 2998
AsuII	TTCGAA	1 cut(s)	634
AvaI	CYCGRG	3 cut(s)	40, 2996, 4505
AvaII	GGWCC	9 cut(s)	32, 236, 757, 869, 1105, 2342, 2524, 2987, 3197
BaeGI	GKGCMC	3 cut(s)	164, 694, 4246
BaeI	ACNNNNGTAYC	2 cut(s)	1078, 1111
BanI	GGYRCC	4 cut(s)	65, 3017, 4176, 4241
BanII	GRGCYC	2 cut(s)	164, 3544
BarI	GAAGNNNNNNTAC	4 cut(s)	2026, 2058, 2847, 2879
BauI	CACGAG	4 cut(s)	3806, 3968, 4122, 4463
BbsI	GAAGAC	1 cut(s)	1857
Bbv12I	GWGCWC	1 cut(s)	694
BbvI	GCAGC	8 cut(s)	982, 1102, 1739, 2052, 4084, 4243, 4405, 4537
BceAI	ACGGC	3 cut(s)	106, 1740, 4538
BcgI	CGANNNNNNTGC	8 cut(s)	1262, 1296, 1825, 1859, 4238, 4272, 4428, 4462
BciT130I	CCWGG	8 cut(s)	36, 992, 1110, 1167, 2809, 3571, 3938, 4102
BciVI	GTATCC	1 cut(s)	2213
BclI	TGATCA	1 cut(s)	3504
BcnI	CCSGG	3 cut(s)	85, 2997, 2998
BcoDI	GTCTC	9 cut(s)	879, 1358, 2706, 3253, 3375, 3544, 4371, 4371, 4448
BfaI	CTAG	9 cut(s)	441, 999, 1152, 1263, 1382, 2244, 2670, 2850, 4148
BfmI	CTRYAG	4 cut(s)	711, 2211, 2487, 4188
BfrI	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
BfuAI	ACCTGC	4 cut(s)	1098, 1655, 2998, 4195
BfuI	GTATCC	1 cut(s)	2213
BglI	GCCNNNNNGGC	1 cut(s)	1301
BglII	AGATCT	2 cut(s)	3643, 3865
BlpI	GCTNAGC	1 cut(s)	336
BmcAI	AGTACT	1 cut(s)	777
Bme18I	GGWCC	9 cut(s)	32, 236, 757, 869, 1105, 2342, 2524, 2987, 3197
BmeT110I	CYCGRG	3 cut(s)	40, 2996, 4505
BmrI	ACTGGG	4 cut(s)	1297, 2017, 2385, 3746
BmsI	GCATC	9 cut(s)	238, 391, 1249, 2638, 3058, 4075, 4156, 4401, 4448
BmuI	ACTGGG	4 cut(s)	1297, 2017, 2385, 3746
BoxI	GACNNNNGTC	1 cut(s)	2816
BpiI	GAAGAC	1 cut(s)	1857
BplI	GAGNNNNNCTC	2 cut(s)	3962, 3994
BpmI	CTGGAG	2 cut(s)	2252, 3959
Bpu10I	CCTNAGC	2 cut(s)	1326, 4346
Bpu1102I	GCTNAGC	1 cut(s)	336
Bpu14I	TTCGAA	1 cut(s)	634
BpuEI	CTTGAG	4 cut(s)	683, 2348, 2852, 3495
BpuMI	CCSGG	3 cut(s)	85, 2997, 2998
BsaBI	GATNNNNATC	2 cut(s)	1682, 3391
BsaHI	GRCGYC	1 cut(s)	2817
BsaI	GGTCTC	2 cut(s)	4371, 4448
BsaWI	WCCGGW	2 cut(s)	238, 4201
BsaXI	ACNNNNNCTCC	4 cut(s)	3171, 3201, 4464, 4494
Bse118I	RCCGGY	1 cut(s)	4044
Bse1I	ACTGG	9 cut(s)	759, 954, 1292, 2012, 2391, 3723, 3752, 4290, 4333
Bse3DI	GCAATG	3 cut(s)	764, 1217, 2290
Bse8I	GATNNNNATC	2 cut(s)	1682, 3391
BseAI	TCCGGA	1 cut(s)	4201
BseBI	CCWGG	8 cut(s)	36, 992, 1110, 1167, 2809, 3571, 3938, 4102
BseGI	GGATG	8 cut(s)	289, 298, 711, 1027, 1683, 3746, 4474, 4571
BseJI	GATNNNNATC	2 cut(s)	1682, 3391
BseMI	GCAATG	3 cut(s)	764, 1217, 2290
BseNI	ACTGG	9 cut(s)	759, 954, 1292, 2012, 2391, 3723, 3752, 4290, 4333
BseRI	GAGGAG	6 cut(s)	278, 302, 404, 407, 3842, 3845
BseSI	GKGCMC	3 cut(s)	164, 694, 4246
BseXI	GCAGC	8 cut(s)	982, 1102, 1739, 2052, 4084, 4243, 4405, 4537
BseYI	CCCAGC	4 cut(s)	477, 821, 3012, 3686
BsgI	GTGCAG	1 cut(s)	2978
BshFI	GGCC	8 cut(s)	39, 162, 225, 819, 1295, 1317, 2950, 4044
BshNI	GGYRCC	4 cut(s)	65, 3017, 4176, 4241
BsiHKAI	GWGCWC	1 cut(s)	694
BsiHKCI	CYCGRG	3 cut(s)	40, 2996, 4505
BsiSI	CCGG	5 cut(s)	84, 239, 2997, 4045, 4202
BslFI	GGGAC	6 cut(s)	101, 1016, 1039, 1118, 1966, 2510
BsmAI	GTCTC	9 cut(s)	879, 1358, 2706, 3253, 3375, 3544, 4371, 4371, 4448
BsmBI	CGTCTC	1 cut(s)	879
BsmFI	GGGAC	6 cut(s)	101, 1016, 1039, 1118, 1966, 2510
BsmI	GAATGC	7 cut(s)	472, 976, 1774, 1798, 2299, 3784, 3950
BsnI	GGCC	8 cut(s)	39, 162, 225, 819, 1295, 1317, 2950, 4044
Bso31I	GGTCTC	2 cut(s)	4371, 4448
BsoBI	CYCGRG	3 cut(s)	40, 2996, 4505
Bsp119I	TTCGAA	1 cut(s)	634
Bsp120I	GGGCCC	1 cut(s)	160
Bsp1286I	GDGCHC	4 cut(s)	164, 694, 3544, 4246
Bsp13I	TCCGGA	1 cut(s)	4201
Bsp1720I	GCTNAGC	1 cut(s)	336
Bsp19I	CCATGG	1 cut(s)	2570
BspANI	GGCC	8 cut(s)	39, 162, 225, 819, 1295, 1317, 2950, 4044
BspEI	TCCGGA	1 cut(s)	4201
BspHI	TCATGA	2 cut(s)	1800, 4592
BspMAI	CTGCAG	2 cut(s)	715, 4192
BspMI	ACCTGC	4 cut(s)	1098, 1655, 2998, 4195
BspPI	GGATC	7 cut(s)	350, 538, 2381, 2660, 3663, 4011, 4276
BspT104I	TTCGAA	1 cut(s)	634
BspT107I	GGYRCC	4 cut(s)	65, 3017, 4176, 4241
BspTI	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
BspTNI	GGTCTC	2 cut(s)	4371, 4448
BsrBI	CCGCTC	1 cut(s)	216
BsrDI	GCAATG	3 cut(s)	764, 1217, 2290
BsrFI	RCCGGY	1 cut(s)	4044
BsrI	ACTGG	9 cut(s)	759, 954, 1292, 2012, 2391, 3723, 3752, 4290, 4333
BssAI	RCCGGY	1 cut(s)	4044
BssNAI	GTATAC	2 cut(s)	2879, 4219
BssNI	GRCGYC	1 cut(s)	2817
BssSI	CACGAG	4 cut(s)	3806, 3968, 4122, 4463
BssT1I	CCWWGG	3 cut(s)	1017, 1691, 2570
Bst1107I	GTATAC	2 cut(s)	2879, 4219
Bst2BI	CACGAG	4 cut(s)	3806, 3968, 4122, 4463
Bst2UI	CCWGG	8 cut(s)	36, 992, 1110, 1167, 2809, 3571, 3938, 4102
Bst6I	CTCTTC	7 cut(s)	675, 789, 2850, 3330, 3356, 3830, 4493
BstACI	GRCGYC	1 cut(s)	2817
BstAFI	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
BstBI	TTCGAA	1 cut(s)	634
BstC8I	GCNNGC	8 cut(s)	28, 208, 227, 3063, 3159, 3608, 3612, 4046
BstDSI	CCRYGG	2 cut(s)	2570, 4554
BstENI	CCTNNNNNAGG	1 cut(s)	2673
BstF5I	GGATG	8 cut(s)	289, 298, 711, 1027, 1683, 3746, 4474, 4571
BstHHI	GCGC	1 cut(s)	4092
BstMAI	GTCTC	9 cut(s)	879, 1358, 2706, 3253, 3375, 3544, 4371, 4371, 4448
BstMWI	GCNNNNNNNGC	8 cut(s)	36, 117, 222, 478, 831, 1301, 1413, 2956
BstNI	CCWGG	8 cut(s)	36, 992, 1110, 1167, 2809, 3571, 3938, 4102
BstNSI	RCATGY	1 cut(s)	3614
BstPAI	GACNNNNGTC	1 cut(s)	2816
BstSFI	CTRYAG	4 cut(s)	711, 2211, 2487, 4188
BstSLI	GKGCMC	3 cut(s)	164, 694, 4246
BstV1I	GCAGC	8 cut(s)	982, 1102, 1739, 2052, 4084, 4243, 4405, 4537
BstV2I	GAAGAC	1 cut(s)	1857
BstX2I	RGATCY	4 cut(s)	530, 2665, 3643, 3865
BstYI	RGATCY	4 cut(s)	530, 2665, 3643, 3865
BstZ17I	GTATAC	2 cut(s)	2879, 4219
BsuI	GTATCC	1 cut(s)	2213
BsuRI	GGCC	8 cut(s)	39, 162, 225, 819, 1295, 1317, 2950, 4044
BtgI	CCRYGG	2 cut(s)	2570, 4554
BtgZI	GCGATG	2 cut(s)	111, 4421
BtsCI	GGATG	8 cut(s)	289, 298, 711, 1027, 1683, 3746, 4474, 4571
BtsI	GCAGTG	2 cut(s)	841, 1146
BtsIMutI	CAGTG	9 cut(s)	841, 871, 961, 1146, 1861, 3443, 3716, 4283, 4326
BveI	ACCTGC	4 cut(s)	1098, 1655, 2998, 4195
Cac8I	GCNNGC	8 cut(s)	28, 208, 227, 3063, 3159, 3608, 3612, 4046
CaiI	CAGNNNCTG	2 cut(s)	1325, 3553
CciI	TCATGA	2 cut(s)	1800, 4592
CfoI	GCGC	1 cut(s)	4092
Cfr10I	RCCGGY	1 cut(s)	4044
Cfr9I	CCCGGG	1 cut(s)	2996
CseI	GACGC	2 cut(s)	2264, 2806
Csp6I	GTAC	8 cut(s)	444, 776, 1095, 1549, 2186, 2492, 3347, 4177
CspCI	CAANNNNNGTGG	2 cut(s)	937, 972
CviQI	GTAC	8 cut(s)	444, 776, 1095, 1549, 2186, 2492, 3347, 4177
EaeI	YGGCCR	1 cut(s)	4042
Eam1104I	CTCTTC	7 cut(s)	675, 789, 2850, 3330, 3356, 3830, 4493
EarI	CTCTTC	7 cut(s)	675, 789, 2850, 3330, 3356, 3830, 4493
EciI	GGCGGA	2 cut(s)	20, 752
Eco130I	CCWWGG	3 cut(s)	1017, 1691, 2570
Eco24I	GRGCYC	2 cut(s)	164, 3544
Eco31I	GGTCTC	2 cut(s)	4371, 4448
Eco32I	GATATC	2 cut(s)	1056, 1864
Eco47I	GGWCC	9 cut(s)	32, 236, 757, 869, 1105, 2342, 2524, 2987, 3197
Eco57I	CTGAAG	5 cut(s)	900, 1629, 3337, 4409, 4535
Eco88I	CYCGRG	3 cut(s)	40, 2996, 4505
EcoNI	CCTNNNNNAGG	1 cut(s)	2673
EcoO109I	RGGNCCY	4 cut(s)	161, 818, 1105, 2987
EcoRI	GAATTC	2 cut(s)	940, 3424
EcoRII	CCWGG	8 cut(s)	34, 990, 1108, 1165, 2807, 3569, 3936, 4100
EcoRV	GATATC	2 cut(s)	1056, 1864
EcoT14I	CCWWGG	3 cut(s)	1017, 1691, 2570
EcoT38I	GRGCYC	2 cut(s)	164, 3544
ErhI	CCWWGG	3 cut(s)	1017, 1691, 2570
Esp3I	CGTCTC	1 cut(s)	879
FaqI	GGGAC	6 cut(s)	101, 1016, 1039, 1118, 1966, 2510
FauI	CCCGC	1 cut(s)	234
FbaI	TGATCA	1 cut(s)	3504
FblI	GTMKAC	2 cut(s)	2878, 4218
FokI	GGATG	8 cut(s)	296, 305, 718, 1034, 1690, 3733, 4481, 4558
FriOI	GRGCYC	2 cut(s)	164, 3544
FspBI	CTAG	9 cut(s)	441, 999, 1152, 1263, 1382, 2244, 2670, 2850, 4148
GlaI	GCGC	1 cut(s)	4091
GsaI	CCCAGC	4 cut(s)	481, 825, 3016, 3690
GsuI	CTGGAG	2 cut(s)	2252, 3959
HaeIII	GGCC	8 cut(s)	39, 162, 225, 819, 1295, 1317, 2950, 4044
HapII	CCGG	5 cut(s)	84, 239, 2997, 4045, 4202
HgaI	GACGC	2 cut(s)	2264, 2806
HhaI	GCGC	1 cut(s)	4092
Hin1I	GRCGYC	1 cut(s)	2817
Hin6I	GCGC	1 cut(s)	4090
HinP1I	GCGC	1 cut(s)	4090
HincII	GTYRAC	1 cut(s)	3085
HindII	GTYRAC	1 cut(s)	3085
HindIII	AAGCTT	3 cut(s)	846, 3104, 3855
HpaII	CCGG	5 cut(s)	84, 239, 2997, 4045, 4202
Hpy166II	GTNNAC	8 cut(s)	692, 839, 2036, 2542, 2879, 3085, 3967, 4219
Hpy8I	GTNNAC	8 cut(s)	692, 839, 2036, 2542, 2879, 3085, 3967, 4219
Hpy99I	CGWCG	2 cut(s)	206, 3285
HpyCH4IV	ACGT	5 cut(s)	1443, 2201, 3283, 4221, 4491
HpyF10VI	GCNNNNNNNGC	8 cut(s)	36, 117, 222, 478, 831, 1301, 1413, 2956
HpySE526I	ACGT	5 cut(s)	1443, 2201, 3283, 4221, 4491
Hsp92I	GRCGYC	1 cut(s)	2817
HspAI	GCGC	1 cut(s)	4090
KflI	GGGWCCC	1 cut(s)	1105
Kpn2I	TCCGGA	1 cut(s)	4201
KpnI	GGTACC	1 cut(s)	4180
KroI	GCCGGC	1 cut(s)	4044
KroNI	GCCGGC	1 cut(s)	4046
Ksp22I	TGATCA	1 cut(s)	3504
LmnI	GCTCC	8 cut(s)	221, 394, 774, 934, 2256, 2331, 2998, 4087
Lsp1109I	GCAGC	8 cut(s)	982, 1102, 1739, 2052, 4084, 4243, 4405, 4537
LweI	GCATC	9 cut(s)	238, 391, 1249, 2638, 3058, 4075, 4156, 4401, 4448
MaeI	CTAG	9 cut(s)	441, 999, 1152, 1263, 1382, 2244, 2670, 2850, 4148
MaeII	ACGT	5 cut(s)	1443, 2201, 3283, 4221, 4491
MaeIII	GTNAC	8 cut(s)	542, 575, 1082, 2458, 2678, 3187, 3974, 4509
MbiI	CCGCTC	1 cut(s)	216
MfeI	CAATTG	3 cut(s)	1764, 1827, 2160
MflI	RGATCY	4 cut(s)	530, 2665, 3643, 3865
MhlI	GDGCHC	4 cut(s)	164, 694, 3544, 4246
MlyI	GAGTC	4 cut(s)	467, 996, 1721, 2675
MmeI	TCCRAC	1 cut(s)	2578
MroI	TCCGGA	1 cut(s)	4201
MroNI	GCCGGC	1 cut(s)	4044
MroXI	GAANNNNTTC	1 cut(s)	1815
MslI	CAYNNNNRTG	8 cut(s)	1371, 2304, 2547, 2553, 2969, 3924, 4071, 4467
MspA1I	CMGCKG	4 cut(s)	1174, 2065, 2486, 3737
MspCI	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
MspI	CCGG	5 cut(s)	84, 239, 2997, 4045, 4202
MunI	CAATTG	3 cut(s)	1764, 1827, 2160
Mva1269I	GAATGC	7 cut(s)	472, 976, 1774, 1798, 2299, 3784, 3950
MvaI	CCWGG	8 cut(s)	36, 992, 1110, 1167, 2809, 3571, 3938, 4102
MwoI	GCNNNNNNNGC	8 cut(s)	36, 117, 222, 478, 831, 1301, 1413, 2956
NaeI	GCCGGC	1 cut(s)	4046
NciI	CCSGG	3 cut(s)	85, 2997, 2998
NcoI	CCATGG	1 cut(s)	2570
NgoMIV	GCCGGC	1 cut(s)	4044
NmeAIII	GCCGAG	1 cut(s)	96
NmuCI	GTSAC	4 cut(s)	575, 1082, 2458, 2678
NspI	RCATGY	1 cut(s)	3614
NspV	TTCGAA	1 cut(s)	634
PaeI	GCATGC	1 cut(s)	3614
PaeR7I	CTCGAG	1 cut(s)	4505
PagI	TCATGA	2 cut(s)	1800, 4592
PctI	GAATGC	7 cut(s)	472, 976, 1774, 1798, 2299, 3784, 3950
PdiI	GCCGGC	1 cut(s)	4046
PdmI	GAANNNNTTC	1 cut(s)	1815
PfeI	GAWTC	6 cut(s)	497, 563, 1162, 1683, 2534, 3485
PflMI	CCANNNNNTGG	1 cut(s)	3570
PfoI	TCCNGGA	1 cut(s)	1165
PleI	GAGTC	4 cut(s)	466, 995, 1720, 2675
PpsI	GAGTC	4 cut(s)	466, 995, 1720, 2675
PpuMI	RGGWCCY	2 cut(s)	1105, 2987
PshAI	GACNNNNGTC	1 cut(s)	2816
PshBI	ATTAAT	1 cut(s)	3165
PsiI	TTATAA	2 cut(s)	1943, 3330
Psp5II	RGGWCCY	2 cut(s)	1105, 2987
Psp6I	CCWGG	8 cut(s)	34, 990, 1108, 1165, 2807, 3569, 3936, 4100
PspFI	CCCAGC	4 cut(s)	477, 821, 3012, 3686
PspGI	CCWGG	8 cut(s)	34, 990, 1108, 1165, 2807, 3569, 3936, 4100
PspOMI	GGGCCC	1 cut(s)	160
PspPPI	RGGWCCY	2 cut(s)	1105, 2987
PspXI	VCTCGAGB	1 cut(s)	4505
PstI	CTGCAG	2 cut(s)	715, 4192
PstNI	CAGNNNCTG	2 cut(s)	1325, 3553
PsuI	RGATCY	4 cut(s)	530, 2665, 3643, 3865
PvuII	CAGCTG	3 cut(s)	2065, 2486, 3737
RsaI	GTAC	8 cut(s)	445, 777, 1096, 1550, 2187, 2493, 3348, 4178
RsaNI	GTAC	8 cut(s)	444, 776, 1095, 1549, 2186, 2492, 3347, 4177
RseI	CAYNNNNRTG	8 cut(s)	1371, 2304, 2547, 2553, 2969, 3924, 4071, 4467
ScaI	AGTACT	1 cut(s)	777
SchI	GAGTC	4 cut(s)	467, 996, 1721, 2675
SduI	GDGCHC	4 cut(s)	164, 694, 3544, 4246
SfaNI	GCATC	9 cut(s)	238, 391, 1249, 2638, 3058, 4075, 4156, 4401, 4448
SfcI	CTRYAG	4 cut(s)	711, 2211, 2487, 4188
Sfr274I	CTCGAG	1 cut(s)	4505
SfuI	TTCGAA	1 cut(s)	634
SinI	GGWCC	9 cut(s)	32, 236, 757, 869, 1105, 2342, 2524, 2987, 3197
SlaI	CTCGAG	1 cut(s)	4505
SmaI	CCCGGG	1 cut(s)	2998
SmiMI	CAYNNNNRTG	8 cut(s)	1371, 2304, 2547, 2553, 2969, 3924, 4071, 4467
SmlI	CTYRAG	9 cut(s)	662, 1006, 2363, 2867, 2882, 3257, 3510, 3620, 4505
SmoI	CTYRAG	9 cut(s)	662, 1006, 2363, 2867, 2882, 3257, 3510, 3620, 4505
SphI	GCATGC	1 cut(s)	3614
SspI	AATATT	4 cut(s)	631, 1228, 1570, 3235
SspMI	CTAG	9 cut(s)	441, 999, 1152, 1263, 1382, 2244, 2670, 2850, 4148
StyI	CCWWGG	3 cut(s)	1017, 1691, 2570
TaiI	ACGT	5 cut(s)	1446, 2204, 3286, 4224, 4494
TatI	WGTACW	5 cut(s)	775, 1548, 2185, 2491, 3346
TauI	GCSGC	3 cut(s)	96, 123, 1664
TfiI	GAWTC	6 cut(s)	497, 563, 1162, 1683, 2534, 3485
TscAI	CASTG	9 cut(s)	841, 871, 961, 1146, 1861, 3450, 3723, 4290, 4333
TseFI	GTSAC	4 cut(s)	575, 1082, 2458, 2678
TseI	GCWGC	8 cut(s)	995, 1115, 1727, 2065, 4097, 4256, 4393, 4550
Tsp45I	GTSAC	4 cut(s)	575, 1082, 2458, 2678
TspGWI	ACGGA	2 cut(s)	624, 861
TspMI	CCCGGG	1 cut(s)	2996
TspRI	CASTG	9 cut(s)	841, 871, 961, 1146, 1861, 3450, 3723, 4290, 4333
Van91I	CCANNNNNTGG	1 cut(s)	3570
Vha464I	CTTAAG	4 cut(s)	1006, 2882, 3257, 3620
VneI	GTGCAC	1 cut(s)	690
VpaK11BI	GGWCC	9 cut(s)	32, 236, 757, 869, 1105, 2342, 2524, 2987, 3197
VspI	ATTAAT	1 cut(s)	3165
XagI	CCTNNNNNAGG	1 cut(s)	2673
XapI	RAATTY	6 cut(s)	940, 2195, 2654, 3424, 3626, 4534
XbaI	TCTAGA	2 cut(s)	2849, 4147
XceI	RCATGY	1 cut(s)	3614
XcmI	CCANNNNNNNNNTGG	1 cut(s)	218
XhoI	CTCGAG	1 cut(s)	4505
XmaI	CCCGGG	1 cut(s)	2996
XmiI	GTMKAC	2 cut(s)	2878, 4218
XmnI	GAANNNNTTC	1 cut(s)	1815
XspI	CTAG	9 cut(s)	441, 999, 1152, 1263, 1382, 2244, 2670, 2850, 4148
ZrmI	AGTACT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.