Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016566

Physical Location & Seq

Genomic Coordinates Forward (+)

27374496 .. 27376466

Gene Structure 1971 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1142900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGCAAATGCAGAGAGTAGGGTTCTCCCTTCGTGTTCCCTGTTCTCACTCTGTTCTCAGGAAGAGAGCCATCCCTATCTCCAAGTTGAAATGCAAAGCGCTGCAACCATTTTCACGTCCTTGCTCTCACGTGCCCAGCGTGTCGGAATCACCACCCTCCACTCTGGTTCAACAGTTCACCAAATTCTGCTACCAGAGAGACCTCCCGAGAGCCATGATTGCCATGGAAGCCATGCAAAGGTGCGAACTTTGGGCCGACTCCGTCGTCTACTCCGAGCTCGTCAAGTGCTGCTTGACTTGGCGGGCTGTTCAGCAGGGTAAGCTCGTCCACAAGCACGTTTTCTCAAATGGGTACCAGCCCAAGACCTTCTTAATCAACGTTTTCATCAACATGTACGTTAAATTTGCTCTCTTGGAGGAAGCGCAGGAACTGTTCGACGAAATGCCTGAGAGAAACGTTGTTTCCTGGACGACTATGATATCGGCATACTCGAATGTCAAATTCAACCGCAAGGCCTTGGAGTTTTTGGTTTTGATGCTTAGAGAAGGTGTTTCGCCTAACAGTTTCACGTATTCTTCGGTTTTGAGAGCTTGCAATGGGTTGCCAAATCTCAAGCAGCTTCATTGTTGTATTTTTAAAGTTGGGTTGGAATCTGACGTCTTTGTTCGGAGTGCTCTCATTGACGTTTACTCGAAATTGGGTGAGTTGCATAATGCGCTGGGCGTTTTCAATGAAATGGTGTCCGGAGATTTGGTCGTTTGGAACTCTATCATTGGAGCGTTTGCTCAGAACAGCGATGGTGATGAGGCTCTAAACCTTTTCAAGAGGATGAAAAGGGCTGGTTTTTTAGCTGAAGAGGCCACACTAACGAGCGTTTTGAGAGCTTGCACGGTGTTGGCGCTTTTAGAATTGGGAAGGCAGGTTCATGTTCATGCTGTGAAGTATGGTCAAGATCTAATTCTGAACAATGCACTTCTAGATATGTATTGCAAGTGTGGGAGTTTGGAAGATGCAAACTCTGCTTTTACCAGGATGGAGAAGAAGGATGTAATCTCTTGGAGCACCATGATTGCAGGGCTAGCGCAAAATGGTTTCAGCCAAGAGGCCCTGAGATTGTTCGAGCAGATGAAAGTTTCCGGGGTGAAACCAAATTACATCACACTTCTTGGGGTTCTGTTTGCTTGTAGTCATGCTGGGCTTTTAGAAGATGGGTGGTACTACTTCCAGAATATGAAGCAGCTTTTTGGAATTGATCCTGGAAGAGAGCACTATGGTTGCGTAATTGATCTTCTTGGAAGGGCAGGGAAACTTGATGAGGCAGCAAGGCTAATACAAGAAATGGAAGGTGAACCAGATGCAGTGACATGGAGGATGTTGCTTGGCGCCTGCAGGGTTCACCGGAATGTAGATCTAGCAGCATATGCTGCCAAACAAATTCTTAAAACGGACCCTGATGATGCCGGAACCTACATTTTACTATCGAACATATATGCCAATTCTCAGAGATGGGAAGATGTGACTGAAGTAAGAAAGAGCATGAAAGCTAGAGGGGTTACTAAAGAACCCGGGTGCAGTTGGATTGAAGTGAACAAACAGATTCATGCTTTTATCTTGGGAGATGACTCACATCCACAAATAGACGAGATCAATAGGCAGCTGAGCTTCTTGATTGATAGACTAATGGCAGTGGGTTACATTCCTGACACGAATTTTGTTTTGCAAGATCTTGAGGGAGAACAGAGGGAAGTTTCACTTCTATCCCACAGCGAGAAATTGGCGATTGTCTTTGGTTTAATGAGCTTGTCAAAGGGGAAGACTGTTAGGATCAGGAAAAACTTAAGGATTTGCGGAGACTGTCATGTATTTGCAAAACTTGTAGCGAAGATTGAGGAGAGAAGTATTGTGATAAGAGATCCCATCCGGTACCATCACTTTCAAGACGGAGTGTGCTCGTGTGGGGATTATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

657

Amino Acids

74.11

Weight (kDa)

8.12

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	151 - 198	1.7e-11	PPR repeat family
PPR_3	PF13812	151 - 198	1.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	250 - 296	2.9e-08	PPR repeat family
PPR	PF01535	252 - 282	1e-07	PPR repeat
PPR_2	PF13041	348 - 388	5.5e-09	PPR repeat family
PPR	PF01535	351 - 381	9.7e-08	PPR repeat
E_motif	PF20431	466 - 528	1.2e-22	E motif
DYW_deaminase	PF14432	564 - 656	6e-37	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0016566)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03880
fragaria_vesca	FvH4_2g16980
malus_domestica	MD05G1142000.v1.1 MD05G1142900.v1.1
prunus_persica	Prupe.8G181700_v2.0.a1
pyrus_communis	pycom05g13740
rosa_chinensis	RchiOBHm_Chr6g0281171
rosa_laevigata	RLG00000012988
rosa_multiflora	Rmu_sc0001705.1_g000021 Rmu_sc0035650.1_g000002
rosa_roxburghii	Rroxscaffold_7G00187240
rosa_rugosa	Rorug06G0139500
rosa_samantha	Rh6DG245900
rosa_wichuraiana	Rw6G021820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	263
AatII	GACGTC	1 cut(s)	660
Acc36I	ACCTGC	1 cut(s)	910
Acc65I	GGTACC	2 cut(s)	351, 1923
AccB1I	GGYRCC	3 cut(s)	351, 1382, 1923
AccI	GTMKAC	1 cut(s)	267
AccIII	TCCGGA	1 cut(s)	743
AciI	CCGC	3 cut(s)	301, 508, 1848
AclI	AACGTT	2 cut(s)	378, 456
AclWI	GGATC	3 cut(s)	1247, 1832, 1907
AcsI	RAATTY	5 cut(s)	182, 401, 500, 1434, 1708
AcuI	CTGAAG	2 cut(s)	873, 1542
AcvI	CACGTG	1 cut(s)	130
AcyI	GRCGYC	2 cut(s)	657, 1383
AfaI	GTAC	4 cut(s)	353, 395, 1217, 1925
AfeI	AGCGCT	1 cut(s)	99
AfiI	CCNNNNNNNGG	1 cut(s)	237
AflII	CTTAAG	1 cut(s)	1837
AflIII	ACRYGT	1 cut(s)	390
AgsI	TTSAA	7 cut(s)	88, 170, 505, 730, 823, 1583, 1937
AjiI	CACGTC	1 cut(s)	116
AjnI	CCWGG	3 cut(s)	464, 1028, 1255
AloI	GAACNNNNNNTCC	2 cut(s)	906, 938
Alw21I	GWGCWC	5 cut(s)	279, 676, 1064, 1269, 1952
Alw26I	GTCTC	2 cut(s)	192, 1845
AlwI	GGATC	3 cut(s)	1247, 1832, 1907
AlwNI	CAGNNNCTG	1 cut(s)	430
Ama87I	CYCGRG	2 cut(s)	205, 1565
Aor13HI	TCCGGA	1 cut(s)	743
Aor51HI	AGCGCT	1 cut(s)	99
AoxI	GGCC	4 cut(s)	252, 513, 858, 1104
ApeKI	GCWGC	8 cut(s)	100, 288, 616, 1237, 1319, 1415, 1424, 1654
ApoI	RAATTY	5 cut(s)	182, 401, 500, 1434, 1708
ArsI	GACNNNNNNTTYG	2 cut(s)	1694, 1726
Asp718I	GGTACC	2 cut(s)	351, 1923
AspLEI	GCGC	6 cut(s)	100, 424, 718, 901, 1084, 1385
AspS9I	GGNCC	3 cut(s)	252, 1105, 1447
AsuC2I	CCSGG	3 cut(s)	1138, 1566, 1567
AsuHPI	GGTGA	7 cut(s)	141, 169, 713, 812, 1153, 1358, 1388
AsuNHI	GCTAGC	1 cut(s)	1078
AvaI	CYCGRG	2 cut(s)	205, 1565
AvaII	GGWCC	1 cut(s)	1447
BaeGI	GKGCMC	1 cut(s)	135
BanI	GGYRCC	3 cut(s)	351, 1382, 1923
BanII	GRGCYC	1 cut(s)	279
BarI	GAAGNNNNNNTAC	2 cut(s)	1272, 1304
BauI	CACGAG	1 cut(s)	1951
BbrPI	CACGTG	1 cut(s)	130
BbsI	GAAGAC	1 cut(s)	1820
Bbv12I	GWGCWC	5 cut(s)	279, 676, 1064, 1269, 1952
BbvI	GCAGC	8 cut(s)	87, 275, 628, 1249, 1331, 1411, 1427, 1666
BccI	CCATC	7 cut(s)	77, 791, 1027, 1202, 1500, 1925, 1935
BciT130I	CCWGG	3 cut(s)	466, 1030, 1257
BcnI	CCSGG	3 cut(s)	1138, 1566, 1567
BcoDI	GTCTC	2 cut(s)	192, 1845
BfaI	CTAG	4 cut(s)	977, 1079, 1412, 1545
BfmI	CTRYAG	1 cut(s)	1387
BfoI	RGCGCY	3 cut(s)	101, 902, 1386
BfrI	CTTAAG	1 cut(s)	1837
BfuAI	ACCTGC	1 cut(s)	910
BglII	AGATCT	3 cut(s)	952, 1408, 1723
BisI	GCNGC	8 cut(s)	101, 289, 617, 1238, 1320, 1416, 1425, 1655
BlpI	GCTNAGC	1 cut(s)	1658
BlsI	GCNGC	8 cut(s)	102, 290, 618, 1239, 1321, 1417, 1426, 1656
Bme1390I	CCNGG	6 cut(s)	466, 1030, 1138, 1257, 1566, 1567
Bme18I	GGWCC	1 cut(s)	1447
BmeT110I	CYCGRG	2 cut(s)	205, 1565
BmgBI	CACGTC	1 cut(s)	116
BmgT120I	GGNCC	3 cut(s)	252, 1105, 1447
BmiI	GGNNCC	5 cut(s)	353, 1384, 1449, 1465, 1925
BmrFI	CCNGG	6 cut(s)	466, 1030, 1138, 1257, 1566, 1567
BmsI	GCATC	4 cut(s)	525, 1000, 1345, 1447
BmtI	GCTAGC	1 cut(s)	1082
BpiI	GAAGAC	1 cut(s)	1820
Bpu1102I	GCTNAGC	1 cut(s)	1658
BpuEI	CTTGAG	2 cut(s)	596, 1748
BpuMI	CCSGG	3 cut(s)	1138, 1566, 1567
BsaAI	YACGTR	2 cut(s)	130, 570
BsaHI	GRCGYC	2 cut(s)	657, 1383
BsaI	GGTCTC	1 cut(s)	192
BsaJI	CCNNGG	4 cut(s)	222, 516, 1137, 1565
BsaWI	WCCGGW	3 cut(s)	743, 1398, 1920
BsaXI	ACNNNNNCTCC	2 cut(s)	738, 768
Bsc4I	CCNNNNNNNGG	1 cut(s)	237
Bse3DI	GCAATG	1 cut(s)	601
BseAI	TCCGGA	1 cut(s)	743
BseBI	CCWGG	3 cut(s)	466, 1030, 1257
BseDI	CCNNGG	4 cut(s)	222, 516, 1137, 1565
BseGI	GGATG	7 cut(s)	69, 834, 1038, 1051, 1377, 1627, 1917
BseLI	CCNNNNNNNGG	1 cut(s)	237
BseMI	GCAATG	1 cut(s)	601
BseMII	CTCAG	6 cut(s)	70, 438, 800, 1100, 1514, 1649
BseRI	GAGGAG	1 cut(s)	1904
BseSI	GKGCMC	1 cut(s)	135
BseXI	GCAGC	8 cut(s)	87, 275, 628, 1249, 1331, 1411, 1427, 1666
BseYI	CCCAGC	3 cut(s)	134, 718, 1193
BsgI	GTGCAG	1 cut(s)	1591
BshFI	GGCC	4 cut(s)	254, 515, 860, 1106
BshNI	GGYRCC	3 cut(s)	351, 1382, 1923
BsiHKAI	GWGCWC	5 cut(s)	279, 676, 1064, 1269, 1952
BsiHKCI	CYCGRG	2 cut(s)	205, 1565
BsiSI	CCGG	6 cut(s)	744, 1137, 1399, 1461, 1566, 1921
BslI	CCNNNNNNNGG	1 cut(s)	237
BsmAI	GTCTC	2 cut(s)	192, 1845
BsnI	GGCC	4 cut(s)	254, 515, 860, 1106
Bso31I	GGTCTC	1 cut(s)	192
BsoBI	CYCGRG	2 cut(s)	205, 1565
Bsp1286I	GDGCHC	6 cut(s)	135, 279, 676, 1064, 1269, 1952
Bsp13I	TCCGGA	1 cut(s)	743
Bsp143I	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
Bsp1720I	GCTNAGC	1 cut(s)	1658
Bsp19I	CCATGG	1 cut(s)	222
BspACI	CCGC	3 cut(s)	301, 508, 1848
BspANI	GGCC	4 cut(s)	254, 515, 860, 1106
BspCNI	CTCAG	6 cut(s)	69, 439, 799, 1101, 1513, 1650
BspEI	TCCGGA	1 cut(s)	743
BspLI	GGNNCC	5 cut(s)	353, 1384, 1449, 1465, 1925
BspMAI	CTGCAG	1 cut(s)	1391
BspMI	ACCTGC	1 cut(s)	910
BspOI	GCTAGC	1 cut(s)	1082
BspPI	GGATC	3 cut(s)	1247, 1832, 1907
BspT107I	GGYRCC	3 cut(s)	351, 1382, 1923
BspTI	CTTAAG	1 cut(s)	1837
BspTNI	GGTCTC	1 cut(s)	192
BsrDI	GCAATG	1 cut(s)	601
BssECI	CCNNGG	4 cut(s)	222, 516, 1137, 1565
BssMI	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
BssNI	GRCGYC	2 cut(s)	657, 1383
BssSI	CACGAG	1 cut(s)	1951
BssT1I	CCWWGG	2 cut(s)	222, 516
Bst2BI	CACGAG	1 cut(s)	1951
Bst2UI	CCWGG	3 cut(s)	466, 1030, 1257
Bst4CI	ACNGT	6 cut(s)	174, 432, 563, 892, 1819, 1856
Bst6I	CTCTTC	3 cut(s)	56, 849, 1255
BstACI	GRCGYC	2 cut(s)	657, 1383
BstAFI	CTTAAG	1 cut(s)	1837
BstAPI	GCANNNNNTGC	3 cut(s)	1019, 1421, 1424
BstBAI	YACGTR	2 cut(s)	130, 570
BstC8I	GCNNGC	5 cut(s)	303, 592, 886, 1080, 1387
BstDEI	CTNAG	7 cut(s)	56, 447, 539, 786, 1109, 1500, 1658
BstDSI	CCRYGG	1 cut(s)	222
BstF5I	GGATG	7 cut(s)	69, 834, 1038, 1051, 1377, 1627, 1917
BstH2I	RGCGCY	3 cut(s)	101, 902, 1386
BstHHI	GCGC	6 cut(s)	100, 424, 718, 901, 1084, 1385
BstKTI	GATC	8 cut(s)	955, 1255, 1288, 1411, 1647, 1726, 1827, 1915
BstMAI	GTCTC	2 cut(s)	192, 1845
BstMBI	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
BstMWI	GCNNNNNNNGC	9 cut(s)	218, 227, 319, 715, 857, 1019, 1079, 1421, 1424
BstNI	CCWGG	3 cut(s)	466, 1030, 1257
BstNSI	RCATGY	1 cut(s)	394
BstSCI	CCNGG	6 cut(s)	464, 1028, 1136, 1255, 1564, 1565
BstSFI	CTRYAG	1 cut(s)	1387
BstSLI	GKGCMC	1 cut(s)	135
BstV1I	GCAGC	8 cut(s)	87, 275, 628, 1249, 1331, 1411, 1427, 1666
BstV2I	GAAGAC	1 cut(s)	1820
BstX2I	RGATCY	4 cut(s)	952, 1408, 1723, 1912
BstYI	RGATCY	4 cut(s)	952, 1408, 1723, 1912
BsuRI	GGCC	4 cut(s)	254, 515, 860, 1106
BtgI	CCRYGG	1 cut(s)	222
BtgZI	GCGATG	1 cut(s)	810
BtrI	CACGTC	1 cut(s)	116
BtsCI	GGATG	7 cut(s)	69, 834, 1038, 1051, 1377, 1627, 1917
BtsI	GCAGTG	2 cut(s)	1365, 1692
BtsIMutI	CAGTG	2 cut(s)	1365, 1692
BveI	ACCTGC	1 cut(s)	910
Cac8I	GCNNGC	5 cut(s)	303, 592, 886, 1080, 1387
CaiI	CAGNNNCTG	1 cut(s)	430
CfoI	GCGC	6 cut(s)	100, 424, 718, 901, 1084, 1385
Cfr13I	GGNCC	3 cut(s)	252, 1105, 1447
Cfr9I	CCCGGG	1 cut(s)	1565
Csp6I	GTAC	4 cut(s)	352, 394, 1216, 1924
CviQI	GTAC	4 cut(s)	352, 394, 1216, 1924
DdeI	CTNAG	7 cut(s)	56, 447, 539, 786, 1109, 1500, 1658
DinI	GGCGCC	1 cut(s)	1384
DpnI	GATC	8 cut(s)	954, 1254, 1287, 1410, 1646, 1725, 1826, 1914
DpnII	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
DraI	TTTAAA	1 cut(s)	637
DrdI	GACNNNNNNGTC	1 cut(s)	263
DseDI	GACNNNNNNGTC	1 cut(s)	263
Eam1104I	CTCTTC	3 cut(s)	56, 849, 1255
EarI	CTCTTC	3 cut(s)	56, 849, 1255
Ecl136II	GAGCTC	1 cut(s)	277
Eco130I	CCWWGG	2 cut(s)	222, 516
Eco147I	AGGCCT	1 cut(s)	515
Eco24I	GRGCYC	1 cut(s)	279
Eco31I	GGTCTC	1 cut(s)	192
Eco32I	GATATC	1 cut(s)	480
Eco47I	GGWCC	1 cut(s)	1447
Eco47III	AGCGCT	1 cut(s)	99
Eco53kI	GAGCTC	1 cut(s)	277
Eco57I	CTGAAG	2 cut(s)	873, 1542
Eco72I	CACGTG	1 cut(s)	130
Eco88I	CYCGRG	2 cut(s)	205, 1565
EcoICRI	GAGCTC	1 cut(s)	277
EcoO109I	RGGNCCY	1 cut(s)	1105
EcoRII	CCWGG	3 cut(s)	464, 1028, 1255
EcoRV	GATATC	1 cut(s)	480
EcoT14I	CCWWGG	2 cut(s)	222, 516
EcoT38I	GRGCYC	1 cut(s)	279
EgeI	GGCGCC	1 cut(s)	1384
EheI	GGCGCC	1 cut(s)	1384
ErhI	CCWWGG	2 cut(s)	222, 516
FalI	AAGNNNNNCTT	6 cut(s)	275, 307, 353, 385, 1737, 1769
FauI	CCCGC	1 cut(s)	294
FauNDI	CATATG	1 cut(s)	1420
FblI	GTMKAC	1 cut(s)	267
Fnu4HI	GCNGC	8 cut(s)	101, 289, 617, 1238, 1320, 1416, 1425, 1655
FokI	GGATG	7 cut(s)	56, 841, 1045, 1058, 1384, 1614, 1904
FriOI	GRGCYC	1 cut(s)	279
Fsp4HI	GCNGC	8 cut(s)	101, 289, 617, 1238, 1320, 1416, 1425, 1655
FspBI	CTAG	4 cut(s)	977, 1079, 1412, 1545
GlaI	GCGC	6 cut(s)	99, 423, 717, 900, 1083, 1384
GluI	GCNGC	8 cut(s)	101, 289, 617, 1238, 1320, 1416, 1425, 1655
GsaI	CCCAGC	3 cut(s)	138, 722, 1197
HaeII	RGCGCY	3 cut(s)	101, 902, 1386
HaeIII	GGCC	4 cut(s)	254, 515, 860, 1106
HapII	CCGG	6 cut(s)	744, 1137, 1399, 1461, 1566, 1921
HhaI	GCGC	6 cut(s)	100, 424, 718, 901, 1084, 1385
Hin1I	GRCGYC	2 cut(s)	657, 1383
Hin6I	GCGC	6 cut(s)	98, 422, 716, 899, 1082, 1383
HinP1I	GCGC	6 cut(s)	98, 422, 716, 899, 1082, 1383
HinfI	GANTC	5 cut(s)	146, 257, 650, 1597, 1622
HpaII	CCGG	6 cut(s)	744, 1137, 1399, 1461, 1566, 1921
HphI	GGTGA	7 cut(s)	141, 169, 713, 812, 1153, 1358, 1388
Hpy166II	GTNNAC	7 cut(s)	177, 268, 328, 688, 1349, 1396, 1588
Hpy188I	TCNGA	7 cut(s)	145, 274, 655, 669, 789, 963, 1503
Hpy8I	GTNNAC	7 cut(s)	177, 268, 328, 688, 1349, 1396, 1588
Hpy99I	CGWCG	2 cut(s)	266, 440
HpyAV	CCTTC	7 cut(s)	38, 376, 539, 909, 1036, 1290, 1337
HpyCH4III	ACNGT	6 cut(s)	174, 432, 563, 892, 1819, 1856
HpyCH4IV	ACGT	9 cut(s)	115, 129, 336, 378, 396, 456, 569, 657, 684
HpyF10VI	GCNNNNNNNGC	9 cut(s)	218, 227, 319, 715, 857, 1019, 1079, 1421, 1424
HpyF3I	CTNAG	7 cut(s)	56, 447, 539, 786, 1109, 1500, 1658
HpySE526I	ACGT	9 cut(s)	115, 129, 336, 378, 396, 456, 569, 657, 684
Hsp92I	GRCGYC	2 cut(s)	657, 1383
HspAI	GCGC	6 cut(s)	98, 422, 716, 899, 1082, 1383
KasI	GGCGCC	1 cut(s)	1382
Kpn2I	TCCGGA	1 cut(s)	743
KpnI	GGTACC	2 cut(s)	355, 1927
Kzo9I	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
LmnI	GCTCC	2 cut(s)	776, 1059
Lsp1109I	GCAGC	8 cut(s)	87, 275, 628, 1249, 1331, 1411, 1427, 1666
LweI	GCATC	4 cut(s)	525, 1000, 1345, 1447
MaeI	CTAG	4 cut(s)	977, 1079, 1412, 1545
MaeII	ACGT	9 cut(s)	115, 129, 336, 378, 396, 456, 569, 657, 684
MaeIII	GTNAC	4 cut(s)	1360, 1516, 1552, 1691
MalI	GATC	8 cut(s)	954, 1254, 1287, 1410, 1646, 1725, 1826, 1914
MboI	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
MflI	RGATCY	4 cut(s)	952, 1408, 1723, 1912
MhlI	GDGCHC	6 cut(s)	135, 279, 676, 1064, 1269, 1952
Mly113I	GGCGCC	1 cut(s)	1383
MlyI	GAGTC	2 cut(s)	251, 1616
MmeI	TCCRAC	3 cut(s)	123, 627, 1556
MroI	TCCGGA	1 cut(s)	743
MseI	TTAA	6 cut(s)	371, 399, 636, 1440, 1793, 1838
MslI	CAYNNNNRTG	3 cut(s)	389, 930, 1401
MspA1I	CMGCKG	1 cut(s)	1657
MspCI	CTTAAG	1 cut(s)	1837
MspI	CCGG	6 cut(s)	744, 1137, 1399, 1461, 1566, 1921
MspR9I	CCNGG	6 cut(s)	466, 1030, 1138, 1257, 1566, 1567
MvaI	CCWGG	3 cut(s)	466, 1030, 1257
MwoI	GCNNNNNNNGC	9 cut(s)	218, 227, 319, 715, 857, 1019, 1079, 1421, 1424
NarI	GGCGCC	1 cut(s)	1383
NciI	CCSGG	3 cut(s)	1138, 1566, 1567
NcoI	CCATGG	1 cut(s)	222
NdeI	CATATG	1 cut(s)	1420
NdeII	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
NheI	GCTAGC	1 cut(s)	1078
NlaIV	GGNNCC	5 cut(s)	353, 1384, 1449, 1465, 1925
NmuCI	GTSAC	2 cut(s)	1360, 1516
NspI	RCATGY	1 cut(s)	394
PceI	AGGCCT	1 cut(s)	515
PciI	ACATGT	1 cut(s)	390
PcsI	WCGNNNNNNNCGW	3 cut(s)	135, 270, 488
PfeI	GAWTC	3 cut(s)	146, 650, 1597
PflFI	GACNNNGTC	1 cut(s)	260
PfoI	TCCNGGA	2 cut(s)	464, 1255
PkrI	GCNGC	8 cut(s)	102, 290, 618, 1239, 1321, 1417, 1426, 1656
PleI	GAGTC	2 cut(s)	251, 1616
PluTI	GGCGCC	1 cut(s)	1386
PmaCI	CACGTG	1 cut(s)	130
PmlI	CACGTG	1 cut(s)	130
PpsI	GAGTC	2 cut(s)	251, 1616
Ppu21I	YACGTR	2 cut(s)	130, 570
PscI	ACATGT	1 cut(s)	390
Psp124BI	GAGCTC	1 cut(s)	279
Psp1406I	AACGTT	2 cut(s)	378, 456
Psp6I	CCWGG	3 cut(s)	464, 1028, 1255
PspCI	CACGTG	1 cut(s)	130
PspFI	CCCAGC	3 cut(s)	134, 718, 1193
PspGI	CCWGG	3 cut(s)	464, 1028, 1255
PspN4I	GGNNCC	5 cut(s)	353, 1384, 1449, 1465, 1925
PspPI	GGNCC	3 cut(s)	252, 1105, 1447
PstI	CTGCAG	1 cut(s)	1391
PstNI	CAGNNNCTG	1 cut(s)	430
PsuI	RGATCY	4 cut(s)	952, 1408, 1723, 1912
PsyI	GACNNNGTC	1 cut(s)	260
PvuII	CAGCTG	1 cut(s)	1657
RsaI	GTAC	4 cut(s)	353, 395, 1217, 1925
RsaNI	GTAC	4 cut(s)	352, 394, 1216, 1924
RseI	CAYNNNNRTG	3 cut(s)	389, 930, 1401
SacI	GAGCTC	1 cut(s)	279
SaqAI	TTAA	6 cut(s)	371, 399, 636, 1440, 1793, 1838
SatI	GCNGC	8 cut(s)	101, 289, 617, 1238, 1320, 1416, 1425, 1655
Sau3AI	GATC	8 cut(s)	952, 1252, 1285, 1408, 1644, 1723, 1824, 1912
Sau96I	GGNCC	3 cut(s)	252, 1105, 1447
SbfI	CCTGCAGG	1 cut(s)	1391
SchI	GAGTC	2 cut(s)	251, 1616
ScrFI	CCNGG	6 cut(s)	466, 1030, 1138, 1257, 1566, 1567
SdaI	CCTGCAGG	1 cut(s)	1391
SduI	GDGCHC	6 cut(s)	135, 279, 676, 1064, 1269, 1952
SfaNI	GCATC	4 cut(s)	525, 1000, 1345, 1447
SfcI	CTRYAG	1 cut(s)	1387
SfoI	GGCGCC	1 cut(s)	1384
SinI	GGWCC	1 cut(s)	1447
SmaI	CCCGGG	1 cut(s)	1567
SmiMI	CAYNNNNRTG	3 cut(s)	389, 930, 1401
SmlI	CTYRAG	3 cut(s)	611, 1727, 1837
SmoI	CTYRAG	3 cut(s)	611, 1727, 1837
Sse8387I	CCTGCAGG	1 cut(s)	1391
SseBI	AGGCCT	1 cut(s)	515
SsiI	CCGC	3 cut(s)	301, 508, 1848
SspDI	GGCGCC	1 cut(s)	1382
SspMI	CTAG	4 cut(s)	977, 1079, 1412, 1545
SstI	GAGCTC	1 cut(s)	279
StuI	AGGCCT	1 cut(s)	515
StyD4I	CCNGG	6 cut(s)	464, 1028, 1136, 1255, 1564, 1565
StyI	CCWWGG	2 cut(s)	222, 516
TaaI	ACNGT	6 cut(s)	174, 432, 563, 892, 1819, 1856
TaiI	ACGT	9 cut(s)	118, 132, 339, 381, 399, 459, 572, 660, 687
TaqI	TCGA	5 cut(s)	435, 491, 692, 1119, 1481
TfiI	GAWTC	3 cut(s)	146, 650, 1597
Tru1I	TTAA	6 cut(s)	371, 399, 636, 1440, 1793, 1838
Tru9I	TTAA	6 cut(s)	371, 399, 636, 1440, 1793, 1838
TscAI	CASTG	2 cut(s)	1365, 1692
TseFI	GTSAC	2 cut(s)	1360, 1516
TseI	GCWGC	8 cut(s)	100, 288, 616, 1237, 1319, 1415, 1424, 1654
Tsp45I	GTSAC	2 cut(s)	1360, 1516
TspGWI	ACGGA	3 cut(s)	250, 1460, 1956
TspMI	CCCGGG	1 cut(s)	1565
TspRI	CASTG	2 cut(s)	1365, 1692
Tth111I	GACNNNGTC	1 cut(s)	260
Vha464I	CTTAAG	1 cut(s)	1837
VpaK11BI	GGWCC	1 cut(s)	1447
XapI	RAATTY	5 cut(s)	182, 401, 500, 1434, 1708
XbaI	TCTAGA	1 cut(s)	976
XceI	RCATGY	1 cut(s)	394
XcmI	CCANNNNNNNNNTGG	1 cut(s)	220
XmaI	CCCGGG	1 cut(s)	1565
XmiI	GTMKAC	1 cut(s)	267
XspI	CTAG	4 cut(s)	977, 1079, 1412, 1545
ZraI	GACGTC	1 cut(s)	658

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.