MD05G1143100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013304
Physical Location & Seq
Forward (+)
27408824 .. 27411126
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1143100.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGAGGACTACATGCGCGGCTGCACGCGGAAGCTGGCGCTCTGGTACACCCGCACCTTCAAGCCCATCATGACCCACGACGAGCTGGAGCCGATCATGGCGACCCTCGGCTTCGTGGGGCTTCCGCCGTCGTCGTCGGCCGCGGCGACGAAGGAGTACGTGTTCTCCTCAGCCGGAGGTTGGCGGAGCAAGTGGGCTCCCCCCGCGGAGGCGCCGCTACCGAGACCGAGGCTGCCGTACCCGAGAATCGACGGGCTTCATATCTATACGTACCGGGCTTTTATTGACGCCGTTAACTTTTACCTCAGGATGAGCGACATCTCCGATCTCTTCCATGTAAGAGGCATGCCGCTCCATGATCTCCATCGAATTAGCGACAGAAACCGGAAGTGGCGCAAAATGGAAGAAGACGACAGTGTGTTTGTTTACAGAGAAGGAACACTAGATCAGGCAACATTCAATCTGTATCACTCTACTAATAAGAACATCAGCAACAGCACTGCCAGTAATGGTAGCAGCGACAATTCTATTGTCATTCGGGAGAAAGGCAACAACACCCCTGTCAGCTGCATTGTTCCGTTGAAAGATATCATAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.73

Weight (kDa)

8.85

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38213
fragaria_vesca FvH4_2g17020
malus_domestica MD05G1143100.v1.1 MD10G1142900.v1.1
prunus_persica Prupe.8G181900_v2.0.a1
pyrus_communis pycom05g13760 pycom10g12280
rosa_chinensis RchiOBHm_Chr6g0281201
rosa_laevigata RLG00000012986
rosa_multiflora Rmu_co8459503.1_g000001 Rmu_sc0001705.1_g000026
rosa_roxburghii Rroxscaffold_7G00187230
rosa_rugosa Rorug06G0139600
rosa_samantha Rh6AG251500 Rh6BG254700 Rh6CG254300 Rh6DG246100
rosa_wichuraiana Rw6G021840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 352
AccII CGCG 4 cut(s) 18, 28, 143, 206
AcoI YGGCCR 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 212, 288
AfaI GTAC 4 cut(s) 47, 158, 239, 272
AfiI CCNNNNNNNGG 2 cut(s) 180, 208
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 61, 460, 583
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 34, 85, 567
AluI AGCT 3 cut(s) 34, 85, 567
Alw26I GTCTC 1 cut(s) 217
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 4 cut(s) 21, 232, 516, 567
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AspLEI GCGC 4 cut(s) 18, 40, 214, 396
AsuC2I CCSGG 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 241
AxyI CCTNAGG 1 cut(s) 305
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 4 cut(s) 8, 219, 528, 554
BccI CCATC 2 cut(s) 74, 372
BceAI ACGGC 3 cut(s) 112, 220, 275
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 2 cut(s) 41, 215
BisI GCNGC 9 cut(s) 19, 22, 141, 144, 215, 233, 350, 517, 568
BlsI GCNGC 9 cut(s) 20, 23, 142, 145, 216, 234, 351, 518, 569
Bme1390I CCNGG 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 241
BmiI GGNNCC 3 cut(s) 90, 198, 213
BmrFI CCNGG 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 414
BpmI CTGGAG 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 169
BpuMI CCSGG 1 cut(s) 275
BsaAI YACGTR 2 cut(s) 160, 270
BsaBI GATNNNNATC 1 cut(s) 363
BsaHI GRCGYC 2 cut(s) 212, 288
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 4 cut(s) 106, 141, 204, 227
BsaWI WCCGGW 1 cut(s) 384
BsaXI ACNNNNNCTCC 4 cut(s) 146, 149, 176, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 208
Bse1I ACTGG 1 cut(s) 504
Bse21I CCTNAGG 1 cut(s) 305
Bse8I GATNNNNATC 1 cut(s) 363
BseDI CCNNGG 4 cut(s) 106, 141, 204, 227
BseGI GGATG 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 180, 208
BseMII CTCAG 2 cut(s) 183, 319
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 1 cut(s) 157
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 8, 219, 528, 554
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 4 cut(s) 18, 28, 143, 206
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 211
BsiEI CGRYCG 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 3 cut(s) 174, 274, 385
BslI CCNNNNNNNGG 2 cut(s) 180, 208
BsmAI GTCTC 1 cut(s) 217
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 4 cut(s) 93, 325, 358, 445
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 182, 318
BspFNI CGCG 4 cut(s) 18, 28, 143, 206
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 3 cut(s) 90, 198, 213
BspT107I GGYRCC 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 217
BsrBI CCGCTC 1 cut(s) 352
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 4 cut(s) 106, 141, 204, 227
BssMI GATC 4 cut(s) 93, 325, 358, 445
BssNI GRCGYC 2 cut(s) 212, 288
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 2 cut(s) 212, 288
BstBAI YACGTR 2 cut(s) 160, 270
BstC8I GCNNGC 3 cut(s) 26, 36, 347
BstDEI CTNAG 2 cut(s) 169, 305
BstDSI CCRYGG 2 cut(s) 141, 204
BstF5I GGATG 1 cut(s) 315
BstFNI CGCG 4 cut(s) 18, 28, 143, 206
BstH2I RGCGCY 2 cut(s) 41, 215
BstHHI GCGC 4 cut(s) 18, 40, 214, 396
BstKTI GATC 4 cut(s) 96, 328, 361, 448
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 4 cut(s) 93, 325, 358, 445
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 16, 349
BstSCI CCNGG 1 cut(s) 273
BstSNI TACGTA 1 cut(s) 270
BstUI CGCG 4 cut(s) 18, 28, 143, 206
BstV1I GCAGC 4 cut(s) 8, 219, 528, 554
BstV2I GAAGAC 1 cut(s) 414
BstZI CGGCCG 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 305
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 2 cut(s) 141, 204
BtsCI GGATG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 421, 498
Cac8I GCNNGC 3 cut(s) 26, 36, 347
CciI TCATGA 1 cut(s) 69
CfoI GCGC 4 cut(s) 18, 40, 214, 396
Cfr42I CCGCGG 2 cut(s) 144, 207
CseI GACGC 1 cut(s) 296
Csp6I GTAC 4 cut(s) 46, 157, 238, 271
CviAII CATG 6 cut(s) 13, 70, 97, 335, 346, 356
CviQI GTAC 4 cut(s) 46, 157, 238, 271
DdeI CTNAG 2 cut(s) 169, 305
DinI GGCGCC 1 cut(s) 213
DpnI GATC 4 cut(s) 95, 327, 360, 447
DpnII GATC 4 cut(s) 93, 325, 358, 445
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
EciI GGCGGA 2 cut(s) 114, 199
EclXI CGGCCG 1 cut(s) 138
Eco105I TACGTA 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 217
Eco32I GATATC 1 cut(s) 589
Eco52I CGGCCG 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 241
EcoRV GATATC 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 199
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
FaeI CATG 6 cut(s) 16, 73, 100, 338, 349, 359
FaiI YATR 9 cut(s) 14, 71, 98, 261, 267, 336, 347, 357, 593
FatI CATG 6 cut(s) 12, 69, 96, 334, 345, 355
FauI CCCGC 2 cut(s) 59, 211
Fnu4HI GCNGC 9 cut(s) 19, 22, 141, 144, 215, 233, 350, 517, 568
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 9 cut(s) 19, 22, 141, 144, 215, 233, 350, 517, 568
FspBI CTAG 1 cut(s) 443
GlaI GCGC 4 cut(s) 17, 39, 213, 395
GluI GCNGC 9 cut(s) 19, 22, 141, 144, 215, 233, 350, 517, 568
GsuI CTGGAG 1 cut(s) 107
HaeII RGCGCY 2 cut(s) 41, 215
HaeIII GGCC 1 cut(s) 140
HapII CCGG 3 cut(s) 174, 274, 385
HgaI GACGC 1 cut(s) 296
HhaI GCGC 4 cut(s) 18, 40, 214, 396
Hin1I GRCGYC 2 cut(s) 212, 288
Hin1II CATG 6 cut(s) 16, 73, 100, 338, 349, 359
Hin6I GCGC 4 cut(s) 16, 38, 212, 394
HinP1I GCGC 4 cut(s) 16, 38, 212, 394
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 246
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 3 cut(s) 174, 274, 385
Hpy166II GTNNAC 3 cut(s) 48, 295, 427
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 3 cut(s) 70, 307, 539
Hpy8I GTNNAC 3 cut(s) 48, 295, 427
Hpy99I CGWCG 6 cut(s) 83, 133, 136, 139, 151, 254
HpyAV CCTTC 3 cut(s) 67, 145, 428
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 2 cut(s) 159, 269
HpyCH4V TGCA 2 cut(s) 24, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpyF3I CTNAG 2 cut(s) 169, 305
HpySE526I ACGT 2 cut(s) 159, 269
Hsp92I GRCGYC 2 cut(s) 212, 288
Hsp92II CATG 6 cut(s) 16, 73, 100, 338, 349, 359
HspAI GCGC 4 cut(s) 16, 38, 212, 394
KasI GGCGCC 1 cut(s) 211
KspAI GTTAAC 1 cut(s) 295
KspI CCGCGG 2 cut(s) 144, 207
Kzo9I GATC 4 cut(s) 93, 325, 358, 445
LmnI GCTCC 4 cut(s) 88, 186, 202, 357
Lsp1109I GCAGC 4 cut(s) 8, 219, 528, 554
MaeI CTAG 1 cut(s) 443
MaeII ACGT 2 cut(s) 159, 269
MalI GATC 4 cut(s) 95, 327, 360, 447
MbiI CCGCTC 1 cut(s) 352
MboI GATC 4 cut(s) 93, 325, 358, 445
MboII GAAGA 3 cut(s) 322, 416, 419
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 2 cut(s) 369, 523
Mly113I GGCGCC 1 cut(s) 212
MnlI CCTC 7 cut(s) 116, 170, 178, 202, 222, 314, 335
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 3 cut(s) 143, 206, 567
MspI CCGG 3 cut(s) 174, 274, 385
MspR9I CCNGG 1 cut(s) 275
MvnI CGCG 4 cut(s) 18, 28, 143, 206
MwoI GCNNNNNNNGC 1 cut(s) 203
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 1 cut(s) 275
NdeII GATC 4 cut(s) 93, 325, 358, 445
NlaIII CATG 6 cut(s) 16, 73, 100, 338, 349, 359
NlaIV GGNNCC 3 cut(s) 90, 198, 213
NmeAIII GCCGAG 1 cut(s) 87
NspI RCATGY 2 cut(s) 16, 349
PaeI GCATGC 1 cut(s) 349
PagI TCATGA 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PfeI GAWTC 1 cut(s) 246
PkrI GCNGC 9 cut(s) 20, 23, 142, 145, 216, 234, 351, 518, 569
PluTI GGCGCC 1 cut(s) 215
Ppu21I YACGTR 2 cut(s) 160, 270
PspN4I GGNNCC 3 cut(s) 90, 198, 213
PvuII CAGCTG 1 cut(s) 567
RsaI GTAC 4 cut(s) 47, 158, 239, 272
RsaNI GTAC 4 cut(s) 46, 157, 238, 271
SacII CCGCGG 2 cut(s) 144, 207
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 9 cut(s) 19, 22, 141, 144, 215, 233, 350, 517, 568
Sau3AI GATC 4 cut(s) 93, 325, 358, 445
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 199
SetI ASST 8 cut(s) 36, 59, 87, 162, 181, 272, 306, 569
SfoI GGCGCC 1 cut(s) 213
Sfr303I CCGCGG 2 cut(s) 144, 207
SgrBI CCGCGG 2 cut(s) 144, 207
SnaBI TACGTA 1 cut(s) 270
SphI GCATGC 1 cut(s) 349
Sse9I AATT 2 cut(s) 369, 523
SspDI GGCGCC 1 cut(s) 211
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 2 cut(s) 162, 272
TaqI TCGA 2 cut(s) 249, 367
TaqII GACCGA 1 cut(s) 241
TasI AATT 2 cut(s) 369, 523
TauI GCSGC 5 cut(s) 21, 143, 146, 217, 352
TfiI GAWTC 1 cut(s) 246
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 421, 505
TseI GCWGC 4 cut(s) 21, 232, 516, 567
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 2 cut(s) 421, 505
XceI RCATGY 2 cut(s) 16, 349
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.