MD10G1142900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013304
Physical Location & Seq
Forward (+)
22896282 .. 22898308
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1142900.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGAGGACTACATGCGCGGCTGCACGCGGAAGCTGGCGCTCTGGTACACCCGGACCTTCAAGCCCATCATGACCCACGACGAGCTCGAGCCGATCATGGCGACCCTCGGGTTCGTGGGGCTTCCCCCGTCTTCGTCGTCGTCGGCTGCGACGAAGGAGTACGTGTTCTCTGCAGCCGGAGGGTGGCGGAGCAAGTGGGCTCCCCCGACGGAGGTGCCGCTGCCGAGGCCGAGGCTGCCGTACCCGAGAATCGATGGGCTTCACATCTATACGTACCGGGCGTTTATTGACGCCGTTAACTGTTCCCTCGAGATGAGCGACATCTCCGATCTCTTCCATGTAAGAGGCATGCCTCTCCATGATCTCCATCGAATTAGCGACAGAAACCGCAAGTGGCGCAAAATGGAAGAAGACGACAGTGTGTTTGTTTACAGAGAAGGGACACTGGATCAAGCAACATTCAACCTTTATCACTCTACTAATAAGAACATCAGCAACAGCACCGCCAGTAATAGCGGCAGCAGCGTTACCACCAATAATTCTATTGTCATTCGGGAGAAGGGGAACAATACCCCTGTCAGCTGCATTGTTCCGTTGAAAGATATCATAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

23.12

Weight (kDa)

8.42

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38213
fragaria_vesca FvH4_2g17020
malus_domestica MD05G1143100.v1.1 MD10G1142900.v1.1
prunus_persica Prupe.8G181900_v2.0.a1
pyrus_communis pycom05g13760 pycom10g12280
rosa_chinensis RchiOBHm_Chr6g0281201
rosa_laevigata RLG00000012986
rosa_multiflora Rmu_co8459503.1_g000001 Rmu_sc0001705.1_g000026
rosa_roxburghii Rroxscaffold_7G00187230
rosa_rugosa Rorug06G0139600
rosa_samantha Rh6AG251500 Rh6BG254700 Rh6CG254300 Rh6DG246100
rosa_wichuraiana Rw6G021840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AccII CGCG 2 cut(s) 18, 28
AciI CCGC 7 cut(s) 18, 28, 187, 218, 388, 504, 516
AclWI GGATC 1 cut(s) 456
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 4 cut(s) 47, 161, 242, 275
AfiI CCNNNNNNNGG 2 cut(s) 183, 211
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 3 cut(s) 61, 463, 598
AloI GAACNNNNNNTCC 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 34, 85, 582
AluI AGCT 3 cut(s) 34, 85, 582
Alw21I GWGCWC 1 cut(s) 87
AlwI GGATC 1 cut(s) 456
Ama87I CYCGRG 4 cut(s) 86, 107, 244, 308
AoxI GGCC 1 cut(s) 227
ApeKI GCWGC 8 cut(s) 21, 146, 173, 220, 235, 519, 522, 582
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AspLEI GCGC 3 cut(s) 18, 40, 399
AspS9I GGNCC 1 cut(s) 54
AsuC2I CCSGG 2 cut(s) 52, 278
AvaI CYCGRG 4 cut(s) 86, 107, 244, 308
AvaII GGWCC 1 cut(s) 54
BanI GGYRCC 1 cut(s) 214
BanII GRGCYC 2 cut(s) 87, 202
BbsI GAAGAC 2 cut(s) 123, 417
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 8 cut(s) 8, 133, 185, 207, 222, 531, 534, 569
BccI CCATC 3 cut(s) 74, 248, 375
BceAI ACGGC 2 cut(s) 223, 278
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BcnI CCSGG 2 cut(s) 52, 278
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 41
Bme1390I CCNGG 2 cut(s) 52, 278
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 4 cut(s) 86, 107, 244, 308
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 2 cut(s) 201, 216
BmrFI CCNGG 2 cut(s) 52, 278
BpiI GAAGAC 2 cut(s) 123, 417
BpuMI CCSGG 2 cut(s) 52, 278
Bsa29I ATCGAT 1 cut(s) 252
BsaAI YACGTR 2 cut(s) 163, 273
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 106, 224, 230
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 211
Bse1I ACTGG 2 cut(s) 450, 507
Bse8I GATNNNNATC 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 106, 224, 230
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 2 cut(s) 183, 211
BseNI ACTGG 2 cut(s) 450, 507
BseXI GCAGC 8 cut(s) 8, 133, 185, 207, 222, 531, 534, 569
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 18, 28
BshFI GGCC 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 214
BshVI ATCGAT 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 4 cut(s) 86, 107, 244, 308
BsiSI CCGG 3 cut(s) 52, 177, 277
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 2 cut(s) 183, 211
BsmFI GGGAC 1 cut(s) 454
BsnI GGCC 1 cut(s) 229
BsoBI CYCGRG 4 cut(s) 86, 107, 244, 308
Bsp1286I GDGCHC 2 cut(s) 87, 202
Bsp143I GATC 4 cut(s) 93, 328, 361, 448
BspACI CCGC 7 cut(s) 18, 28, 187, 218, 388, 504, 516
BspANI GGCC 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 252
BspFNI CGCG 2 cut(s) 18, 28
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 201, 216
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 214
BsrI ACTGG 2 cut(s) 450, 507
BssECI CCNNGG 3 cut(s) 106, 224, 230
BssMI GATC 4 cut(s) 93, 328, 361, 448
BssNI GRCGYC 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 302, 419
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 2 cut(s) 163, 273
BstC8I GCNNGC 3 cut(s) 26, 36, 350
BstFNI CGCG 2 cut(s) 18, 28
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 3 cut(s) 18, 40, 399
BstKTI GATC 4 cut(s) 96, 331, 364, 451
BstMBI GATC 4 cut(s) 93, 328, 361, 448
BstMWI GCNNNNNNNGC 4 cut(s) 226, 235, 396, 522
BstNSI RCATGY 2 cut(s) 16, 352
BstSCI CCNGG 2 cut(s) 50, 276
BstSFI CTRYAG 1 cut(s) 171
BstSNI TACGTA 1 cut(s) 273
BstUI CGCG 2 cut(s) 18, 28
BstV1I GCAGC 8 cut(s) 8, 133, 185, 207, 222, 531, 534, 569
BstV2I GAAGAC 2 cut(s) 123, 417
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 1 cut(s) 229
BsuTUI ATCGAT 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 424, 443
Cac8I GCNNGC 3 cut(s) 26, 36, 350
CciI TCATGA 1 cut(s) 69
CfoI GCGC 3 cut(s) 18, 40, 399
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 1 cut(s) 299
Csp6I GTAC 4 cut(s) 46, 160, 241, 274
CviAII CATG 6 cut(s) 13, 70, 97, 338, 349, 359
CviQI GTAC 4 cut(s) 46, 160, 241, 274
DpnI GATC 4 cut(s) 95, 330, 363, 450
DpnII GATC 4 cut(s) 93, 328, 361, 448
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 273
Eco24I GRGCYC 2 cut(s) 87, 202
Eco32I GATATC 1 cut(s) 604
Eco47I GGWCC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 4 cut(s) 86, 107, 244, 308
EcoICRI GAGCTC 1 cut(s) 85
EcoRV GATATC 1 cut(s) 604
EcoT38I GRGCYC 2 cut(s) 87, 202
FaeI CATG 6 cut(s) 16, 73, 100, 341, 352, 362
FaiI YATR 8 cut(s) 14, 71, 98, 270, 339, 350, 360, 608
FaqI GGGAC 1 cut(s) 454
FatI CATG 6 cut(s) 12, 69, 96, 337, 348, 358
FriOI GRGCYC 2 cut(s) 87, 202
GlaI GCGC 3 cut(s) 17, 39, 398
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 1 cut(s) 229
HapII CCGG 3 cut(s) 52, 177, 277
HgaI GACGC 1 cut(s) 299
HhaI GCGC 3 cut(s) 18, 40, 399
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 6 cut(s) 16, 73, 100, 341, 352, 362
Hin6I GCGC 3 cut(s) 16, 38, 397
HinP1I GCGC 3 cut(s) 16, 38, 397
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 1 cut(s) 249
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 3 cut(s) 52, 177, 277
Hpy166II GTNNAC 3 cut(s) 48, 298, 430
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 3 cut(s) 70, 310, 554
Hpy8I GTNNAC 3 cut(s) 48, 298, 430
Hpy99I CGWCG 6 cut(s) 83, 139, 142, 145, 154, 211
HpyAV CCTTC 4 cut(s) 67, 148, 431, 553
HpyCH4III ACNGT 2 cut(s) 302, 419
HpyCH4IV ACGT 2 cut(s) 162, 272
HpyCH4V TGCA 3 cut(s) 24, 173, 585
HpyF10VI GCNNNNNNNGC 4 cut(s) 226, 235, 396, 522
HpySE526I ACGT 2 cut(s) 162, 272
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 6 cut(s) 16, 73, 100, 341, 352, 362
HspAI GCGC 3 cut(s) 16, 38, 397
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 4 cut(s) 93, 328, 361, 448
LmnI GCTCC 2 cut(s) 189, 205
LpnPI CCDG 8 cut(s) 20, 28, 65, 190, 290, 431, 520, 588
Lsp1109I GCAGC 8 cut(s) 8, 133, 185, 207, 222, 531, 534, 569
MaeII ACGT 2 cut(s) 162, 272
MaeIII GTNAC 1 cut(s) 526
MalI GATC 4 cut(s) 95, 330, 363, 450
MboI GATC 4 cut(s) 93, 328, 361, 448
MboII GAAGA 4 cut(s) 123, 325, 419, 422
MhlI GDGCHC 2 cut(s) 87, 202
MluCI AATT 2 cut(s) 372, 538
MnlI CCTC 8 cut(s) 116, 173, 205, 219, 225, 317, 338, 363
MseI TTAA 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 220, 582
MspI CCGG 3 cut(s) 52, 177, 277
MspR9I CCNGG 2 cut(s) 52, 278
MvnI CGCG 2 cut(s) 18, 28
MwoI GCNNNNNNNGC 4 cut(s) 226, 235, 396, 522
NciI CCSGG 2 cut(s) 52, 278
NdeII GATC 4 cut(s) 93, 328, 361, 448
NlaIII CATG 6 cut(s) 16, 73, 100, 341, 352, 362
NlaIV GGNNCC 2 cut(s) 201, 216
NmeAIII GCCGAG 2 cut(s) 249, 255
NspI RCATGY 2 cut(s) 16, 352
PaeI GCATGC 1 cut(s) 352
PaeR7I CTCGAG 2 cut(s) 86, 308
PagI TCATGA 1 cut(s) 69
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 146, 149, 278, 315
PfeI GAWTC 1 cut(s) 249
Ppu21I YACGTR 2 cut(s) 163, 273
Psp124BI GAGCTC 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 201, 216
PspPI GGNCC 1 cut(s) 54
PspXI VCTCGAGB 1 cut(s) 86
PstI CTGCAG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 582
RsaI GTAC 4 cut(s) 47, 161, 242, 275
RsaNI GTAC 4 cut(s) 46, 160, 241, 274
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 297
Sau3AI GATC 4 cut(s) 93, 328, 361, 448
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 52, 278
SduI GDGCHC 2 cut(s) 87, 202
SetI ASST 8 cut(s) 36, 59, 87, 165, 216, 275, 468, 584
SfcI CTRYAG 1 cut(s) 171
Sfr274I CTCGAG 2 cut(s) 86, 308
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 2 cut(s) 86, 308
SmlI CTYRAG 2 cut(s) 86, 308
SmoI CTYRAG 2 cut(s) 86, 308
SnaBI TACGTA 1 cut(s) 273
SphI GCATGC 1 cut(s) 352
Sse9I AATT 2 cut(s) 372, 538
SsiI CCGC 7 cut(s) 18, 28, 187, 218, 388, 504, 516
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 50, 276
TaaI ACNGT 2 cut(s) 302, 419
TaiI ACGT 2 cut(s) 165, 275
TaqI TCGA 4 cut(s) 87, 252, 309, 370
TasI AATT 2 cut(s) 372, 538
TauI GCSGC 3 cut(s) 21, 220, 519
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 2 cut(s) 424, 450
TseI GCWGC 8 cut(s) 21, 146, 173, 220, 235, 519, 522, 582
TspGWI ACGGA 2 cut(s) 224, 582
TspRI CASTG 2 cut(s) 424, 450
VpaK11BI GGWCC 1 cut(s) 54
XceI RCATGY 2 cut(s) 16, 352
XhoI CTCGAG 2 cut(s) 86, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.