MD05G1153400.v1.1

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005822
Physical Location & Seq
Forward (+)
28429586 .. 28433499
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1153400.v1.1.491

Sequence Viewer

Length: 1653 bp
ATGCTTTATCAGATCGAAGTTAGCATATCCTTTGCCATGGATAATACCCATTCAGTTTCCACGTTGATAGATTGTACAACTTCTAAGATACAACAGCTTCAGAAAGCATTTGCTGAACTTGAGAGTCACAGAGCTGTAACCCTGAACCTGAAGTGGAAAGAACTTGAAGCGCATTTCCGTGGTCTTGAAAAGTCCTTAAAGAGGCGTTTCGATGAGCTGGAAAACCAAGAAAAGGAGTTTGAAACCCGAACAGTTGAAGCTCAAAAAATGTTAGCAAAGCGGCAAGCTGCCGTTGTGGCCAAGGAACAAGCTTCACTGGACACTCTTCAAGCAAAGAGAGATGCTGCTACATATGCTATTACTAATGCTCGAGAGAAGCAAAGGAAGGTATCAACTGAGGAGCCTTCTGTTGTCACTGATGATGGCCAAGGTGAGCAGCCAAGTGTGGAAGAGAAACCACCAGATGTGATGGTTTCTGAAATTAACTTCGAAGAAGTGAAAAGTCCTGAAATGGGGAGTAGTATAGAGGTGATATCCTATCCCCAGTTAGTGAAACTGTGTGAACAGATGGACTCAGAAGGGCTCCACAAATTTATATCAGATAACCGGAAGAACCTTGCTTCCATAAGGGAGGAGATTCCACTTGCATTAAGAGCTGCATCCAACCCTGCCCTATTTGTTTTGGAATCTTTGGAAGACTTTTATCGCCTCGAAGGGCCCAATACGGACGGGAAGAAGGATGCAAACCTGTTGGGTGTCCGTCGAACTTGTATCATGTTGATGGAGTGTCTGAGCACACTGCTAGCAAACCCAGAATTAGCCTCTGCATCTGATATAATTACAGAGGAGGTTAAGGATCTGGCAGAGGCAATTGCTGAAGAATGGAAGCCGAAGTTGGATGCTCTTGACATGGATGCCAGCAATGGGAACTCACTTGAGGCTCACGCCTTTTTGCAACTTCTTGGAACTTTTGGTATTACCTCTGGTTTTGATGAGGAAGAACTATTCAGGCTAATACCGATGGTTTCACGTCGTCGCCAAGCAGCTGACCTTTGCCGTTCTCTTGGATTGACTGAGAGAATGCCAGGTGTTATTGACGTGTTAGTGAATAGTGGACGGCAAATTGATGCAGTTAACTTGGCTTTTGCATTTGAACTGACGGAGAAGTTCCCTCCTGTGCCTTTACTGAAGTCCTACTTGAAAGAGGCAAGAAAAACTTCACCAGTCAAATCTGGAAATGCATCTCCCACAGCACAGAATGAAGTGAACGACCGTGAGTTGGCTGCCCTTAAGGCGGTGCTCAAGTCCATTGAAGAGCATAAGCTTGAGGAGCAGTATCCAGTGGATCCTCTTCAGAAACGGGTTCTTCAGCTGGAAAAGGCCAAGGCAGACAAGAAGAGGGTGGCTGAAGCATCAAAGCCTCAGCCCAAGAGACCTCGTGCTAACGGTGTTGGATATGCTCCCCGCGTCACTAATGCTGTTGCTGACAAGACTTTCTATCCCAGAGTTGCTGAAAACAGGTACCCGCAGTATGTGTACGACAGACAGTTTGTTTACCCGGGACCTGCTGACAACCATTACAACATGTCTCCTGCTCATGGAAACTACTTTGCAACCGGCTACCAGTATCAGCCTGCGGCGTATCTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

551

Amino Acids

61.76

Weight (kDa)

5.51

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 179 - 469 1.3e-112 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1573
Acc65I GGTACC 1 cut(s) 1521
AccB1I GGYRCC 1 cut(s) 1521
AccII CGCG 1 cut(s) 1467
AciI CCGC 5 cut(s) 280, 1295, 1465, 1526, 1637
AclWI GGATC 3 cut(s) 864, 1340, 1353
AcoI YGGCCR 2 cut(s) 297, 424
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 7 cut(s) 83, 170, 897, 1208, 1337, 1352, 1428
AfaI GTAC 3 cut(s) 76, 1523, 1538
AfiI CCNNNNNNNGG 7 cut(s) 201, 232, 512, 924, 1279, 1294, 1598
AflII CTTAAG 1 cut(s) 1289
AflIII ACRYGT 2 cut(s) 1098, 1584
AgsI TTSAA 8 cut(s) 167, 188, 242, 257, 329, 1154, 1201, 1313
AjiI CACGTC 2 cut(s) 1031, 1099
AjnI CCWGG 1 cut(s) 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
Alw21I GWGCWC 2 cut(s) 797, 1302
Alw26I GTCTC 2 cut(s) 1426, 1593
AlwI GGATC 3 cut(s) 864, 1340, 1353
Ama87I CYCGRG 2 cut(s) 369, 1558
AoxI GGCC 4 cut(s) 297, 424, 716, 1380
ApaI GGGCCC 1 cut(s) 720
ApeKI GCWGC 6 cut(s) 287, 344, 436, 656, 1043, 1283
ApoI RAATTY 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 1532, 1564
Asp700I GAANNNNTTC 1 cut(s) 1216
Asp718I GGTACC 1 cut(s) 1521
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 3 cut(s) 716, 717, 1562
AsuC2I CCSGG 2 cut(s) 1559, 1560
AsuHPI GGTGA 3 cut(s) 443, 541, 1212
AsuII TTCGAA 1 cut(s) 489
AsuNHI GCTAGC 1 cut(s) 802
AvaI CYCGRG 2 cut(s) 369, 1558
AvaII GGWCC 1 cut(s) 1562
BaeGI GKGCMC 1 cut(s) 720
BalI TGGCCA 2 cut(s) 299, 426
BamHI GGATCC 1 cut(s) 1345
BanI GGYRCC 1 cut(s) 1521
BanII GRGCYC 2 cut(s) 585, 720
BauI CACGAG 1 cut(s) 1437
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 797, 1302
BbvCI CCTCAGC 1 cut(s) 1422
BbvI GCAGC 6 cut(s) 274, 331, 448, 643, 1055, 1270
BccI CCATC 5 cut(s) 416, 463, 562, 775, 1015
BceAI ACGGC 3 cut(s) 275, 1041, 1133
BciT130I CCWGG 1 cut(s) 1086
BciVI GTATCC 1 cut(s) 1347
BcnI CCSGG 2 cut(s) 1559, 1560
BcoDI GTCTC 2 cut(s) 1426, 1593
BfaI CTAG 1 cut(s) 803
BfrI CTTAAG 1 cut(s) 1289
BfuAI ACCTGC 1 cut(s) 1573
BfuI GTATCC 1 cut(s) 1347
BglI GCCNNNNNGGC 2 cut(s) 296, 1292
BisI GCNGC 8 cut(s) 281, 288, 345, 437, 657, 1044, 1284, 1638
BlsI GCNGC 8 cut(s) 282, 289, 346, 438, 658, 1045, 1285, 1639
Bme1390I CCNGG 3 cut(s) 1086, 1559, 1560
Bme18I GGWCC 1 cut(s) 1562
BmeT110I CYCGRG 2 cut(s) 369, 1558
BmgBI CACGTC 2 cut(s) 1031, 1099
BmgT120I GGNCC 3 cut(s) 716, 717, 1562
BmiI GGNNCC 6 cut(s) 402, 584, 718, 1347, 1523, 1563
BmrFI CCNGG 3 cut(s) 1086, 1559, 1560
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 9 cut(s) 331, 668, 730, 836, 889, 904, 1117, 1250, 1421
BmtI GCTAGC 1 cut(s) 806
BmuI ACTGGG 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 702
Bpu10I CCTNAGC 1 cut(s) 1422
Bpu14I TTCGAA 1 cut(s) 489
BpuEI CTTGAG 4 cut(s) 140, 956, 1286, 1346
BpuMI CCSGG 2 cut(s) 1559, 1560
BsaI GGTCTC 1 cut(s) 1426
BsaJI CCNNGG 6 cut(s) 36, 178, 300, 427, 1383, 1558
BsaWI WCCGGW 1 cut(s) 606
Bsc4I CCNNNNNNNGG 7 cut(s) 201, 232, 512, 924, 1279, 1294, 1598
Bse118I RCCGGY 1 cut(s) 1616
Bse1I ACTGG 5 cut(s) 321, 544, 1223, 1340, 1624
Bse3DI GCAATG 1 cut(s) 928
BseBI CCWGG 1 cut(s) 1086
BseDI CCNNGG 6 cut(s) 36, 178, 300, 427, 1383, 1558
BseGI GGATG 4 cut(s) 659, 745, 904, 919
BseLI CCNNNNNNNGG 7 cut(s) 201, 232, 512, 924, 1279, 1294, 1598
BseMI GCAATG 1 cut(s) 928
BseMII CTCAG 5 cut(s) 387, 588, 782, 1065, 1436
BseNI ACTGG 5 cut(s) 321, 544, 1223, 1340, 1624
BseRI GAGGAG 4 cut(s) 413, 647, 860, 1343
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 6 cut(s) 274, 331, 448, 643, 1055, 1270
Bsh1236I CGCG 1 cut(s) 1467
Bsh1285I CGRYCG 1 cut(s) 1273
BshFI GGCC 4 cut(s) 299, 426, 718, 1382
BshNI GGYRCC 1 cut(s) 1521
BsiEI CGRYCG 1 cut(s) 1273
BsiHKAI GWGCWC 2 cut(s) 797, 1302
BsiHKCI CYCGRG 2 cut(s) 369, 1558
BsiSI CCGG 3 cut(s) 607, 1559, 1617
BslFI GGGAC 1 cut(s) 1575
BslI CCNNNNNNNGG 7 cut(s) 201, 232, 512, 924, 1279, 1294, 1598
BsmAI GTCTC 2 cut(s) 1426, 1593
BsmFI GGGAC 1 cut(s) 1575
BsmI GAATGC 1 cut(s) 1086
BsnI GGCC 4 cut(s) 299, 426, 718, 1382
Bso31I GGTCTC 1 cut(s) 1426
BsoBI CYCGRG 2 cut(s) 369, 1558
Bsp119I TTCGAA 1 cut(s) 489
Bsp120I GGGCCC 1 cut(s) 716
Bsp1286I GDGCHC 4 cut(s) 585, 720, 797, 1302
Bsp1407I TGTACA 1 cut(s) 74
Bsp143I GATC 3 cut(s) 12, 856, 1345
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 5 cut(s) 280, 1295, 1465, 1526, 1637
BspANI GGCC 4 cut(s) 299, 426, 718, 1382
BspCNI CTCAG 5 cut(s) 388, 587, 783, 1066, 1435
BspFNI CGCG 1 cut(s) 1467
BspLI GGNNCC 6 cut(s) 402, 584, 718, 1347, 1523, 1563
BspMI ACCTGC 1 cut(s) 1573
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 3 cut(s) 864, 1340, 1353
BspQI GCTCTTC 1 cut(s) 1308
BspT104I TTCGAA 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 1521
BspTI CTTAAG 1 cut(s) 1289
BspTNI GGTCTC 1 cut(s) 1426
BsrDI GCAATG 1 cut(s) 928
BsrFI RCCGGY 1 cut(s) 1616
BsrGI TGTACA 1 cut(s) 74
BsrI ACTGG 5 cut(s) 321, 544, 1223, 1340, 1624
BssAI RCCGGY 1 cut(s) 1616
BssECI CCNNGG 6 cut(s) 36, 178, 300, 427, 1383, 1558
BssMI GATC 3 cut(s) 12, 856, 1345
BssSI CACGAG 1 cut(s) 1437
BssT1I CCWWGG 4 cut(s) 36, 300, 427, 1383
Bst2BI CACGAG 1 cut(s) 1437
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 5 cut(s) 253, 558, 1274, 1448, 1548
Bst6I CTCTTC 5 cut(s) 330, 444, 1308, 1356, 1391
BstAFI CTTAAG 1 cut(s) 1289
BstAUI TGTACA 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 285, 804, 919, 1635
BstDEI CTNAG 6 cut(s) 84, 396, 574, 791, 1074, 1422
BstDSI CCRYGG 2 cut(s) 36, 178
BstENI CCTNNNNNAGG 1 cut(s) 199
BstF5I GGATG 4 cut(s) 659, 745, 904, 919
BstFNI CGCG 1 cut(s) 1467
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 3 cut(s) 15, 859, 1348
BstMAI GTCTC 2 cut(s) 1426, 1593
BstMBI GATC 3 cut(s) 12, 856, 1345
BstMCI CGRYCG 1 cut(s) 1273
BstMWI GCNNNNNNNGC 5 cut(s) 296, 353, 653, 1292, 1330
BstNI CCWGG 1 cut(s) 1086
BstNSI RCATGY 1 cut(s) 1588
BstSCI CCNGG 3 cut(s) 1084, 1557, 1558
BstSLI GKGCMC 1 cut(s) 720
BstUI CGCG 1 cut(s) 1467
BstV1I GCAGC 6 cut(s) 274, 331, 448, 643, 1055, 1270
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 2 cut(s) 856, 1345
BstYI RGATCY 2 cut(s) 856, 1345
BsuI GTATCC 1 cut(s) 1347
BsuRI GGCC 4 cut(s) 299, 426, 718, 1382
BtgI CCRYGG 2 cut(s) 36, 178
BtrI CACGTC 2 cut(s) 1031, 1099
BtsCI GGATG 4 cut(s) 659, 745, 904, 919
BtsI GCAGTG 1 cut(s) 797
BtsIMutI CAGTG 4 cut(s) 314, 414, 797, 1347
BveI ACCTGC 1 cut(s) 1573
Cac8I GCNNGC 4 cut(s) 285, 804, 919, 1635
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 1616
Cfr13I GGNCC 3 cut(s) 716, 717, 1562
Cfr9I CCCGGG 1 cut(s) 1558
CseI GACGC 1 cut(s) 1456
Csp6I GTAC 3 cut(s) 75, 1522, 1537
CviAII CATG 5 cut(s) 37, 775, 910, 1585, 1598
CviQI GTAC 3 cut(s) 75, 1522, 1537
DdeI CTNAG 6 cut(s) 84, 396, 574, 791, 1074, 1422
DpnI GATC 3 cut(s) 14, 858, 1347
DpnII GATC 3 cut(s) 12, 856, 1345
EaeI YGGCCR 2 cut(s) 297, 424
Eam1104I CTCTTC 5 cut(s) 330, 444, 1308, 1356, 1391
EarI CTCTTC 5 cut(s) 330, 444, 1308, 1356, 1391
Eco130I CCWWGG 4 cut(s) 36, 300, 427, 1383
Eco24I GRGCYC 2 cut(s) 585, 720
Eco31I GGTCTC 1 cut(s) 1426
Eco32I GATATC 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 1562
Eco57I CTGAAG 7 cut(s) 83, 170, 897, 1208, 1337, 1352, 1428
Eco88I CYCGRG 2 cut(s) 369, 1558
EcoNI CCTNNNNNAGG 1 cut(s) 199
EcoO109I RGGNCCY 2 cut(s) 716, 1562
EcoRII CCWGG 1 cut(s) 1084
EcoRV GATATC 1 cut(s) 534
EcoT14I CCWWGG 4 cut(s) 36, 300, 427, 1383
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 585, 720
ErhI CCWWGG 4 cut(s) 36, 300, 427, 1383
FaeI CATG 5 cut(s) 40, 778, 913, 1588, 1601
FalI AAGNNNNNCTT 4 cut(s) 1181, 1213, 1201, 1233
FaqI GGGAC 1 cut(s) 1575
FatI CATG 5 cut(s) 36, 774, 909, 1584, 1597
FauI CCCGC 2 cut(s) 1472, 1533
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 8 cut(s) 281, 288, 345, 437, 657, 1044, 1284, 1638
FokI GGATG 4 cut(s) 646, 752, 911, 926
FriOI GRGCYC 2 cut(s) 585, 720
Fsp4HI GCNGC 8 cut(s) 281, 288, 345, 437, 657, 1044, 1284, 1638
FspBI CTAG 1 cut(s) 803
GlaI GCGC 1 cut(s) 171
GluI GCNGC 8 cut(s) 281, 288, 345, 437, 657, 1044, 1284, 1638
HaeIII GGCC 4 cut(s) 299, 426, 718, 1382
HapII CCGG 3 cut(s) 607, 1559, 1617
HgaI GACGC 1 cut(s) 1456
HhaI GCGC 1 cut(s) 172
Hin1II CATG 5 cut(s) 40, 778, 913, 1588, 1601
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HindIII AAGCTT 2 cut(s) 309, 1322
HinfI GANTC 4 cut(s) 124, 572, 637, 686
HpaI GTTAAC 1 cut(s) 1135
HpaII CCGG 3 cut(s) 607, 1559, 1617
HphI GGTGA 3 cut(s) 443, 541, 1212
Hpy166II GTNNAC 6 cut(s) 563, 1115, 1135, 1267, 1537, 1555
Hpy188I TCNGA 8 cut(s) 12, 102, 478, 577, 601, 792, 832, 1356
Hpy188III TCNNGA 5 cut(s) 185, 371, 506, 905, 1233
Hpy8I GTNNAC 6 cut(s) 563, 1115, 1135, 1267, 1537, 1555
Hpy99I CGWCG 3 cut(s) 765, 1035, 1038
HpyAV CCTTC 5 cut(s) 379, 414, 572, 707, 730
HpyCH4III ACNGT 5 cut(s) 253, 558, 1274, 1448, 1548
HpyCH4IV ACGT 3 cut(s) 62, 1030, 1098
HpyCH4V TGCA 9 cut(s) 647, 659, 743, 827, 955, 1130, 1148, 1241, 1613
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 353, 653, 1292, 1330
HpyF3I CTNAG 6 cut(s) 84, 396, 574, 791, 1074, 1422
HpySE526I ACGT 3 cut(s) 62, 1030, 1098
Hsp92II CATG 5 cut(s) 40, 778, 913, 1588, 1601
HspAI GCGC 1 cut(s) 170
KpnI GGTACC 1 cut(s) 1525
KspAI GTTAAC 1 cut(s) 1135
Kzo9I GATC 3 cut(s) 12, 856, 1345
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 4 cut(s) 400, 588, 1330, 1465
Lsp1109I GCAGC 6 cut(s) 274, 331, 448, 643, 1055, 1270
LweI GCATC 9 cut(s) 331, 668, 730, 836, 889, 904, 1117, 1250, 1421
MaeI CTAG 1 cut(s) 803
MaeII ACGT 3 cut(s) 62, 1030, 1098
MaeIII GTNAC 4 cut(s) 125, 136, 412, 1468
MalI GATC 3 cut(s) 14, 858, 1347
MboI GATC 3 cut(s) 12, 856, 1345
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 2 cut(s) 856, 1345
MhlI GDGCHC 4 cut(s) 585, 720, 797, 1302
MlsI TGGCCA 2 cut(s) 299, 426
MluCI AATT 6 cut(s) 480, 590, 815, 837, 870, 1122
MluNI TGGCCA 2 cut(s) 299, 426
MlyI GAGTC 2 cut(s) 133, 566
MmeI TCCRAC 3 cut(s) 687, 876, 1432
Mox20I TGGCCA 2 cut(s) 299, 426
Mph1103I ATGCAT 1 cut(s) 1243
MroXI GAANNNNTTC 1 cut(s) 1216
MscI TGGCCA 2 cut(s) 299, 426
MseI TTAA 6 cut(s) 197, 483, 650, 852, 1134, 1290
MslI CAYNNNNRTG 2 cut(s) 177, 779
Msp20I TGGCCA 2 cut(s) 299, 426
MspA1I CMGCKG 2 cut(s) 1046, 1372
MspCI CTTAAG 1 cut(s) 1289
MspI CCGG 3 cut(s) 607, 1559, 1617
MspR9I CCNGG 3 cut(s) 1086, 1559, 1560
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 1 cut(s) 1086
MvaI CCWGG 1 cut(s) 1086
MvnI CGCG 1 cut(s) 1467
MwoI GCNNNNNNNGC 5 cut(s) 296, 353, 653, 1292, 1330
NciI CCSGG 2 cut(s) 1559, 1560
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 352
NdeII GATC 3 cut(s) 12, 856, 1345
NheI GCTAGC 1 cut(s) 802
NlaIII CATG 5 cut(s) 40, 778, 913, 1588, 1601
NlaIV GGNNCC 6 cut(s) 402, 584, 718, 1347, 1523, 1563
NmuCI GTSAC 3 cut(s) 125, 412, 1468
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 1 cut(s) 1588
NspV TTCGAA 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 369
PciI ACATGT 1 cut(s) 1584
PciSI GCTCTTC 1 cut(s) 1308
PctI GAATGC 1 cut(s) 1086
PdmI GAANNNNTTC 1 cut(s) 1216
PfeI GAWTC 2 cut(s) 637, 686
PkrI GCNGC 8 cut(s) 282, 289, 346, 438, 658, 1045, 1285, 1639
PleI GAGTC 2 cut(s) 132, 566
PpsI GAGTC 2 cut(s) 132, 566
PpuMI RGGWCCY 1 cut(s) 1562
PscI ACATGT 1 cut(s) 1584
Psp5II RGGWCCY 1 cut(s) 1562
Psp6I CCWGG 1 cut(s) 1084
PspGI CCWGG 1 cut(s) 1084
PspN4I GGNNCC 6 cut(s) 402, 584, 718, 1347, 1523, 1563
PspOMI GGGCCC 1 cut(s) 716
PspPI GGNCC 3 cut(s) 716, 717, 1562
PspPPI RGGWCCY 1 cut(s) 1562
PsuI RGATCY 2 cut(s) 856, 1345
PvuII CAGCTG 2 cut(s) 1046, 1372
RsaI GTAC 3 cut(s) 76, 1523, 1538
RsaNI GTAC 3 cut(s) 75, 1522, 1537
RseI CAYNNNNRTG 2 cut(s) 177, 779
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 6 cut(s) 197, 483, 650, 852, 1134, 1290
SatI GCNGC 8 cut(s) 281, 288, 345, 437, 657, 1044, 1284, 1638
Sau3AI GATC 3 cut(s) 12, 856, 1345
Sau96I GGNCC 3 cut(s) 716, 717, 1562
SchI GAGTC 2 cut(s) 133, 566
ScrFI CCNGG 3 cut(s) 1086, 1559, 1560
SduI GDGCHC 4 cut(s) 585, 720, 797, 1302
SfaNI GCATC 9 cut(s) 331, 668, 730, 836, 889, 904, 1117, 1250, 1421
Sfr274I CTCGAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 489
SinI GGWCC 1 cut(s) 1562
SlaI CTCGAG 1 cut(s) 369
SmaI CCCGGG 1 cut(s) 1560
SmiMI CAYNNNNRTG 2 cut(s) 177, 779
SmlI CTYRAG 6 cut(s) 119, 369, 935, 1289, 1301, 1325
SmoI CTYRAG 6 cut(s) 119, 369, 935, 1289, 1301, 1325
Sse9I AATT 6 cut(s) 480, 590, 815, 837, 870, 1122
SsiI CCGC 5 cut(s) 280, 1295, 1465, 1526, 1637
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 3 cut(s) 1084, 1557, 1558
StyI CCWWGG 4 cut(s) 36, 300, 427, 1383
TaaI ACNGT 5 cut(s) 253, 558, 1274, 1448, 1548
TaiI ACGT 3 cut(s) 65, 1033, 1101
TaqI TCGA 6 cut(s) 15, 210, 370, 489, 711, 763
TasI AATT 6 cut(s) 480, 590, 815, 837, 870, 1122
TatI WGTACW 1 cut(s) 74
TauI GCSGC 2 cut(s) 283, 1640
TfiI GAWTC 2 cut(s) 637, 686
Tru1I TTAA 6 cut(s) 197, 483, 650, 852, 1134, 1290
Tru9I TTAA 6 cut(s) 197, 483, 650, 852, 1134, 1290
TscAI CASTG 4 cut(s) 321, 421, 804, 1347
TseFI GTSAC 3 cut(s) 125, 412, 1468
TseI GCWGC 6 cut(s) 287, 344, 436, 656, 1043, 1283
Tsp45I GTSAC 3 cut(s) 125, 412, 1468
TspDTI ATGAA 1 cut(s) 1275
TspGWI ACGGA 4 cut(s) 167, 740, 749, 1175
TspMI CCCGGG 1 cut(s) 1558
TspRI CASTG 4 cut(s) 321, 421, 804, 1347
Vha464I CTTAAG 1 cut(s) 1289
VpaK11BI GGWCC 1 cut(s) 1562
XagI CCTNNNNNAGG 1 cut(s) 199
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 1588
XhoI CTCGAG 1 cut(s) 369
XmaI CCCGGG 1 cut(s) 1558
XmnI GAANNNNTTC 1 cut(s) 1216
XspI CTAG 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.