Rh6DG253400

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005822
Physical Location & Seq
Forward (+)
44258581 .. 44263198
4618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG253400.1

Sequence Viewer

Length: 1233 bp
ATGGAAGATACACACTCAGTTTCTACGCTCATAGATTCTACAACCTCCAAGATACACCAGCTTCAGAAGGCATTTGCTGAACTTGAGGGTCACCGAGCGGTAACCCTAAACTTGAAGTGGAAAGAACTTGAAGAGCACTTCCATGGGCTTGAGAAGTCCTTAAAGAGGCGTTTTCATGAGCTGGAAGGACAAGAAAAGGAGTTTGAAACTAGGACAGTGGAAGCGCAAAGAATGCTAGAAAAGCGGCAAGCAGCTGTTGTCGCCAAGGAAGAAGCTCAACTGGAAACTCTTCAAGAGAAGAGAGATGCTGCCATATATGCCATCACTAATGCTCGAGAGAAGCAAAGGAAGGTATCGACTGAGGAGTCTTCTGTTGTCACTGATGACGGCCAAGGCGGATCACCAACTGTTGAAGAGAAACCACCAGATGTGATGGTTTCTGATAGTAACTTGGAAGAAGTGACAAGTGCTGAAAATGGGAGCATAGAGGGGATGTCCTATCCCCAGTTAGTGAACTTTTGTGAACAGATGGACTCAGAAGGCCTCCACAAGTTTATATCAGATAACCGAAAGAACCTTGCTGCAATAAGGGAGGAGATTCCACTTGCATTTAGAGCTGCGGCCAACCCTGCCCTCTTTGTTTTGGAATCTCTGGAAGACTTTTACCGCATGGAAGGACCCACTATGGATGTGAAGAAGGATTCCAACCTGTTGGGTATCCGTCGAACCTGCATAATGTTGATGGAGTGTCTGAGCATATTGTTAACAAGCCCAGAGTTAGCCTCTGCTTCTGATATTATTACAGAGGAGGTTAAGGATATGGCAGAGATGATTGCTGAAGAATGGAAGCCAAAGTTGGATGCTCTTGACATGGATGCTAGCAATGGGAACTCACTGGAGGCTCATGCCTTTTTGCAGCTTCTAGCTACTTTTGGTATTGCTTCCGGTTTTGATGAAGAAGAATTGTTCAGGCTAATTCCAATGGTTTCACGTCGTCGCCAAACAGCTGACCTTTGCCGTTCACTTGGATTGACCGAGAGAATGCCAGGTGTTATTGAAGTATTAGTGAACAGTGGAAGACAAATTGATGCAGTTAACCTGGCTTTTGCATTCGAACTGACAGAGCAGTTCTCTCCTGTGCCTTTGCTAAAGTCCTACTTGAAGGAGGCAAGAAAAGCTTCTTCACCAGTCAAACCTGGGAATGCATCTCCTACTGCACAGGTGTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

410

Amino Acids

45.84

Weight (kDa)

4.9

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 166 - 407 3.2e-87 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 364
Acc36I ACCTGC 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 98
AciI CCGC 5 cut(s) 98, 244, 396, 620, 667
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 2 cut(s) 388, 621
AcuI CTGAAG 2 cut(s) 47, 858
AfiI CCNNNNNNNGG 1 cut(s) 165
AgsI TTSAA 7 cut(s) 115, 131, 206, 293, 413, 1058, 1162
AjiI CACGTC 1 cut(s) 992
AjnI CCWGG 3 cut(s) 1045, 1098, 1195
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
AleI CACNNNNGTG 1 cut(s) 1222
AluBI AGCT 9 cut(s) 61, 181, 254, 275, 617, 919, 926, 1007, 1178
AluI AGCT 9 cut(s) 61, 181, 254, 275, 617, 919, 926, 1007, 1178
Alw21I GWGCWC 1 cut(s) 138
AlwI GGATC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 333
AoxI GGCC 3 cut(s) 388, 541, 621
ApeKI GCWGC 5 cut(s) 251, 308, 581, 617, 916
Asp700I GAANNNNTTC 2 cut(s) 288, 1177
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 677
AsuHPI GGTGA 3 cut(s) 83, 393, 1176
AsuII TTCGAA 1 cut(s) 1113
AsuNHI GCTAGC 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 1 cut(s) 677
BbsI GAAGAC 3 cut(s) 360, 663, 1084
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 5 cut(s) 263, 295, 568, 604, 928
BccI CCATC 4 cut(s) 329, 427, 523, 736
BceAI ACGGC 2 cut(s) 403, 1002
BciT130I CCWGG 3 cut(s) 1047, 1100, 1197
BciVI GTATCC 1 cut(s) 728
BfaI CTAG 4 cut(s) 210, 236, 879, 923
BfuAI ACCTGC 1 cut(s) 737
BfuI GTATCC 1 cut(s) 728
BisI GCNGC 7 cut(s) 245, 252, 309, 582, 618, 621, 917
BlsI GCNGC 7 cut(s) 246, 253, 310, 583, 619, 622, 918
Bme1390I CCNGG 3 cut(s) 1047, 1100, 1197
Bme18I GGWCC 1 cut(s) 677
BmeT110I CYCGRG 1 cut(s) 333
BmgBI CACGTC 1 cut(s) 992
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 1 cut(s) 679
BmrFI CCNGG 3 cut(s) 1047, 1100, 1197
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 5 cut(s) 295, 850, 865, 1078, 1214
BmtI GCTAGC 1 cut(s) 882
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 3 cut(s) 360, 663, 1084
BplI GAGNNNNNCTC 4 cut(s) 767, 799, 1115, 1147
BpmI CTGGAG 1 cut(s) 917
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 2 cut(s) 104, 170
BsaJI CCNNGG 4 cut(s) 142, 264, 391, 1196
BsaWI WCCGGW 1 cut(s) 944
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse1I ACTGG 4 cut(s) 285, 505, 900, 1187
Bse3DI GCAATG 1 cut(s) 889
BseBI CCWGG 3 cut(s) 1047, 1100, 1197
BseDI CCNNGG 4 cut(s) 142, 264, 391, 1196
BseGI GGATG 4 cut(s) 498, 694, 865, 880
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMI GCAATG 1 cut(s) 889
BseMII CTCAG 4 cut(s) 30, 351, 549, 743
BseNI ACTGG 4 cut(s) 285, 505, 900, 1187
BseRI GAGGAG 3 cut(s) 377, 608, 821
BseXI GCAGC 5 cut(s) 263, 295, 568, 604, 928
BsgI GTGCAG 1 cut(s) 1200
BshFI GGCC 3 cut(s) 390, 543, 623
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 945
BslI CCNNNNNNNGG 1 cut(s) 165
BsmI GAATGC 4 cut(s) 237, 1047, 1109, 1207
BsnI GGCC 3 cut(s) 390, 543, 623
BsoBI CYCGRG 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 398
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 5 cut(s) 98, 244, 396, 620, 667
BspANI GGCC 3 cut(s) 390, 543, 623
BspCNI CTCAG 4 cut(s) 29, 352, 548, 744
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 737
BspOI GCTAGC 1 cut(s) 882
BspPI GGATC 1 cut(s) 406
BspQI GCTCTTC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 1113
BsrBI CCGCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 889
BsrI ACTGG 4 cut(s) 285, 505, 900, 1187
BssECI CCNNGG 4 cut(s) 142, 264, 391, 1196
BssMI GATC 1 cut(s) 398
BssT1I CCWWGG 3 cut(s) 142, 264, 391
Bst2UI CCWGG 3 cut(s) 1047, 1100, 1197
Bst4CI ACNGT 3 cut(s) 217, 409, 1073
Bst6I CTCTTC 4 cut(s) 126, 293, 294, 408
BstAPI GCANNNNNTGC 1 cut(s) 232
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 2 cut(s) 249, 880
BstDEI CTNAG 4 cut(s) 16, 360, 535, 752
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 2 cut(s) 89, 100
BstENI CCTNNNNNAGG 1 cut(s) 163
BstF5I GGATG 4 cut(s) 498, 694, 865, 880
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 1 cut(s) 401
BstMBI GATC 1 cut(s) 398
BstMWI GCNNNNNNNGC 7 cut(s) 232, 241, 260, 317, 614, 629, 1175
BstNI CCWGG 3 cut(s) 1047, 1100, 1197
BstPI GGTNACC 2 cut(s) 89, 100
BstSCI CCNGG 3 cut(s) 1045, 1098, 1195
BstV1I GCAGC 5 cut(s) 263, 295, 568, 604, 928
BstV2I GAAGAC 3 cut(s) 360, 663, 1084
BstXI CCANNNNNNTGG 1 cut(s) 712
BsuI GTATCC 1 cut(s) 728
BsuRI GGCC 3 cut(s) 390, 543, 623
BtgI CCRYGG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 992
BtsCI GGATG 4 cut(s) 498, 694, 865, 880
BtsIMutI CAGTG 4 cut(s) 222, 378, 893, 1078
BveI ACCTGC 1 cut(s) 737
Cac8I GCNNGC 2 cut(s) 249, 880
CciI TCATGA 1 cut(s) 175
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 677
CviAII CATG 5 cut(s) 143, 176, 670, 871, 905
DdeI CTNAG 4 cut(s) 16, 360, 535, 752
DpnI GATC 1 cut(s) 400
DpnII GATC 1 cut(s) 398
DrdI GACNNNNNNGTC 1 cut(s) 364
DseDI GACNNNNNNGTC 1 cut(s) 364
EaeI YGGCCR 2 cut(s) 388, 621
Eam1104I CTCTTC 4 cut(s) 126, 293, 294, 408
EarI CTCTTC 4 cut(s) 126, 293, 294, 408
EciI GGCGGA 1 cut(s) 411
Eco130I CCWWGG 3 cut(s) 142, 264, 391
Eco147I AGGCCT 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 2 cut(s) 47, 858
Eco88I CYCGRG 1 cut(s) 333
Eco91I GGTNACC 2 cut(s) 89, 100
EcoNI CCTNNNNNAGG 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 677
EcoO65I GGTNACC 2 cut(s) 89, 100
EcoRII CCWGG 3 cut(s) 1045, 1098, 1195
EcoT14I CCWWGG 3 cut(s) 142, 264, 391
EcoT22I ATGCAT 1 cut(s) 1207
ErhI CCWWGG 3 cut(s) 142, 264, 391
FaeI CATG 5 cut(s) 146, 179, 673, 874, 908
FalI AAGNNNNNCTT 4 cut(s) 1142, 1174, 1162, 1194
FatI CATG 5 cut(s) 142, 175, 669, 870, 904
Fnu4HI GCNGC 7 cut(s) 245, 252, 309, 582, 618, 621, 917
FokI GGATG 4 cut(s) 505, 701, 872, 887
Fsp4HI GCNGC 7 cut(s) 245, 252, 309, 582, 618, 621, 917
FspBI CTAG 4 cut(s) 210, 236, 879, 923
GlaI GCGC 1 cut(s) 225
GluI GCNGC 7 cut(s) 245, 252, 309, 582, 618, 621, 917
GsuI CTGGAG 1 cut(s) 917
HaeIII GGCC 3 cut(s) 390, 543, 623
HapII CCGG 1 cut(s) 945
HhaI GCGC 1 cut(s) 226
Hin1II CATG 5 cut(s) 146, 179, 673, 874, 908
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 2 cut(s) 765, 1096
HindII GTYRAC 2 cut(s) 765, 1096
HindIII AAGCTT 1 cut(s) 1176
HinfI GANTC 6 cut(s) 35, 365, 533, 598, 647, 701
HpaI GTTAAC 2 cut(s) 765, 1096
HpaII CCGG 1 cut(s) 945
HphI GGTGA 3 cut(s) 83, 393, 1176
Hpy166II GTNNAC 6 cut(s) 514, 524, 765, 1022, 1069, 1096
Hpy188I TCNGA 6 cut(s) 66, 442, 538, 562, 753, 793
Hpy188III TCNNGA 5 cut(s) 176, 293, 335, 653, 866
Hpy8I GTNNAC 6 cut(s) 514, 524, 765, 1022, 1069, 1096
Hpy99I CGWCG 3 cut(s) 726, 996, 999
HpyAV CCTTC 7 cut(s) 61, 179, 343, 533, 668, 691, 1156
HpyCH4III ACNGT 3 cut(s) 217, 409, 1073
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 8 cut(s) 584, 608, 732, 916, 1091, 1109, 1205, 1217
HpyF10VI GCNNNNNNNGC 7 cut(s) 232, 241, 260, 317, 614, 629, 1175
HpyF3I CTNAG 4 cut(s) 16, 360, 535, 752
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 5 cut(s) 146, 179, 673, 874, 908
HspAI GCGC 1 cut(s) 224
KspAI GTTAAC 2 cut(s) 765, 1096
Kzo9I GATC 1 cut(s) 398
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 5 cut(s) 263, 295, 568, 604, 928
LweI GCATC 5 cut(s) 295, 850, 865, 1078, 1214
MaeI CTAG 4 cut(s) 210, 236, 879, 923
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 5 cut(s) 89, 100, 376, 446, 460
MalI GATC 1 cut(s) 400
MbiI CCGCTC 1 cut(s) 98
MboI GATC 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 962, 975, 1083
MlyI GAGTC 2 cut(s) 374, 527
MmeI TCCRAC 2 cut(s) 729, 837
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 2 cut(s) 288, 1177
MseI TTAA 4 cut(s) 161, 764, 813, 1095
MslI CAYNNNNRTG 2 cut(s) 141, 1222
MspA1I CMGCKG 2 cut(s) 254, 1007
MspI CCGG 1 cut(s) 945
MspR9I CCNGG 3 cut(s) 1047, 1100, 1197
Mva1269I GAATGC 4 cut(s) 237, 1047, 1109, 1207
MvaI CCWGG 3 cut(s) 1047, 1100, 1197
MwoI GCNNNNNNNGC 7 cut(s) 232, 241, 260, 317, 614, 629, 1175
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 398
NheI GCTAGC 1 cut(s) 878
NlaIII CATG 5 cut(s) 146, 179, 673, 874, 908
NlaIV GGNNCC 1 cut(s) 679
NmuCI GTSAC 3 cut(s) 89, 376, 460
NsiI ATGCAT 1 cut(s) 1207
NspV TTCGAA 1 cut(s) 1113
OliI CACNNNNGTG 1 cut(s) 1222
PaeR7I CTCGAG 1 cut(s) 333
PagI TCATGA 1 cut(s) 175
PceI AGGCCT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 126
PctI GAATGC 4 cut(s) 237, 1047, 1109, 1207
PdmI GAANNNNTTC 2 cut(s) 288, 1177
PfeI GAWTC 4 cut(s) 35, 598, 647, 701
PkrI GCNGC 7 cut(s) 246, 253, 310, 583, 619, 622, 918
PleI GAGTC 2 cut(s) 373, 527
PpsI GAGTC 2 cut(s) 373, 527
PpuMI RGGWCCY 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 677
Psp6I CCWGG 3 cut(s) 1045, 1098, 1195
PspEI GGTNACC 2 cut(s) 89, 100
PspGI CCWGG 3 cut(s) 1045, 1098, 1195
PspN4I GGNNCC 1 cut(s) 679
PspPI GGNCC 1 cut(s) 677
PspPPI RGGWCCY 1 cut(s) 677
PvuII CAGCTG 2 cut(s) 254, 1007
RseI CAYNNNNRTG 2 cut(s) 141, 1222
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 4 cut(s) 161, 764, 813, 1095
SatI GCNGC 7 cut(s) 245, 252, 309, 582, 618, 621, 917
Sau3AI GATC 1 cut(s) 398
Sau96I GGNCC 1 cut(s) 677
SchI GAGTC 2 cut(s) 374, 527
ScrFI CCNGG 3 cut(s) 1047, 1100, 1197
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 5 cut(s) 295, 850, 865, 1078, 1214
Sfr274I CTCGAG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 1113
SinI GGWCC 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 333
SmiMI CAYNNNNRTG 2 cut(s) 141, 1222
SmlI CTYRAG 3 cut(s) 83, 149, 333
SmoI CTYRAG 3 cut(s) 83, 149, 333
Sse9I AATT 3 cut(s) 962, 975, 1083
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 5 cut(s) 98, 244, 396, 620, 667
SspMI CTAG 4 cut(s) 210, 236, 879, 923
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 1045, 1098, 1195
StyI CCWWGG 3 cut(s) 142, 264, 391
TaaI ACNGT 3 cut(s) 217, 409, 1073
TaiI ACGT 1 cut(s) 994
TaqI TCGA 4 cut(s) 334, 356, 724, 1113
TaqII GACCGA 1 cut(s) 1049
TasI AATT 3 cut(s) 962, 975, 1083
TauI GCSGC 2 cut(s) 247, 623
TfiI GAWTC 4 cut(s) 35, 598, 647, 701
Tru1I TTAA 4 cut(s) 161, 764, 813, 1095
Tru9I TTAA 4 cut(s) 161, 764, 813, 1095
TscAI CASTG 4 cut(s) 222, 385, 900, 1078
TseFI GTSAC 3 cut(s) 89, 376, 460
TseI GCWGC 5 cut(s) 251, 308, 581, 617, 916
Tsp45I GTSAC 3 cut(s) 89, 376, 460
TspDTI ATGAA 2 cut(s) 164, 969
TspGWI ACGGA 1 cut(s) 710
TspRI CASTG 4 cut(s) 222, 385, 900, 1078
VpaK11BI GGWCC 1 cut(s) 677
XagI CCTNNNNNAGG 1 cut(s) 163
XhoI CTCGAG 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 288, 1177
XspI CTAG 4 cut(s) 210, 236, 879, 923
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.