MD05G1170300.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013546
Physical Location & Seq
Forward (+)
29987233 .. 29988725
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1170300.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGGAGAGGTATCCAAATCGGGATTGAAAGAGGAGGAGATTGAGCTGGATTTGGGGCTGTCGATCGGCGGCAGCTTTGGAAGGCCGGAGAACTCGAAGCTGGCCAACGGGTTCGATGTAGCTCCGAGAGAGAACGAGCACCAAACGCTGCCGTCCCAACCGCACGCGCCTTCTCCGTCGCCGATAGAACAGTCGGAGCCGGAGAGCGCTCGTCTGGATCCGCAGACGAAGCGGGAGATGCAGGCGTTGAGAAGGCAGGAAGCGAAGAGGAAGCGGGAGGAGAAGAAAATCCGGGGACTGAACGGTTCCCAAGCGGAAGAGCAGCCTGGGGTGAAGAAAGAGAGAACGGAGACGAATGTGAATGTGATCAATCTGAATTTGAGCAACGGACAAAGGGAGCTGCGCTACCCGGTTCAGTACCCGTACCCGCCGGTCCATTTTGTTCCGTACACGAATCCTTGCGTGATGCCGTGCTGGGGCCCAAGCGGCGGCGGGGGTTTTCGGCATTTTCAGGCGCATAAGCCGATGTTGAATAACGGGTGTGAGAAGGAGCAGAATGGTGGATCGGGTAAAACGGCATCGGTAAATGGTGGGTCGGGTAAAACGGCGTCGGTAAATGGTGGGTTGGGTAAGAAACCGGCGTGGACAAATGTTTCACCAGTGTGTAGTTCCTCCACCGTGTCAGATAATCAAAGCGCAGCTTCTCATGAAGGTGGTGGAAGCAGTGAGAGCAGAAGCCATTCAGGCCAGAGCATACAAGAAAACATAGAGCCGGTTTCAAATTCCTTGGAATCCAATCCCAAAGGGAATGAACAAAAACTGAATCCAAAAACAGAACCCAAATCAGAAAACGCGGAACCGGCTCAAATCCTAAAACCCAAGTCACCGAAACAAGATTCTTCTCCTCCATTTCCTCCACTGAAGGAAATTGCCAAACCGGACCCAATTTCCAAGCCTCCGAAACCCCCGATGAGCAAAAATGGCAGCAATGCGGTTTCCCTTCCCCAAATGCCTTACGTCTCGACGACCGGGACCGGCCCGAACGGGAAAACGGTTCATGGGTTGTTGTACAGATACACGAAATCTGAGATCACCATCGTGTGTGTCTGCCATGGAACCGCATTCTCGCCGGCTGAATTCGTGCAGCATGCCGGAGGCACCGATGTTTCCCAGCCGCTGAGGCACATCACTGTGATCCCATCTGCTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAR Jas

Protein Analysis

405

Amino Acids

43.3

Weight (kDa)

8.74

Isoelectric Point (pI)

61.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAR PF07897 11 - 33 3.1e-07 Ethylene-responsive binding factor-associated repression
TDBD PF16135 365 - 397 1.6e-08 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013546)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07255
fragaria_vesca FvH4_2g18440 FvH4_2g18440
malus_domestica MD05G1170300.v1.1 MD10G1158500.v1.1
prunus_persica Prupe.8G197500_v2.0.a1
pyrus_communis pycom05g15750
rosa_chinensis RchiOBHm_Chr6g0283921
rosa_laevigata RLG00000012786
rosa_multiflora Rmu_ssc0000442.1_g000021
rosa_roxburghii Rroxscaffold_6G00425120 Rroxscaffold_7G00185060
rosa_rugosa Rorug06G0158500
rosa_samantha Rh6AG271500 Rh6BG272700 Rh6CG273900 Rh6DG266800
rosa_wichuraiana Rw6G023420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1157
AccII CGCG 2 cut(s) 167, 854
AclWI GGATC 4 cut(s) 212, 225, 571, 1189
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 3 cut(s) 376, 781, 1136
AcuI CTGAAG 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 608
AfaI GTAC 4 cut(s) 419, 425, 449, 1070
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 476, 488
AgsI TTSAA 3 cut(s) 28, 532, 780
AjnI CCWGG 1 cut(s) 325
AleI CACNNNNGTG 2 cut(s) 661, 1097
AluBI AGCT 6 cut(s) 46, 75, 100, 122, 400, 701
AluI AGCT 6 cut(s) 46, 75, 100, 122, 400, 701
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 344, 1024
AlwI GGATC 4 cut(s) 212, 225, 571, 1189
AlwNI CAGNNNCTG 1 cut(s) 1177
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 5 cut(s) 83, 102, 478, 745, 1036
ApaI GGGCCC 1 cut(s) 482
ApeKI GCWGC 7 cut(s) 72, 148, 322, 400, 698, 984, 1144
ApoI RAATTY 3 cut(s) 376, 781, 1136
Asp700I GAANNNNTTC 1 cut(s) 739
AspLEI GCGC 5 cut(s) 169, 209, 405, 517, 698
AspS9I GGNCC 6 cut(s) 433, 478, 479, 940, 1032, 1037
AsuC2I CCSGG 3 cut(s) 293, 410, 1030
AsuHPI GGTGA 4 cut(s) 343, 648, 876, 1084
AvaII GGWCC 3 cut(s) 433, 940, 1032
BaeGI GKGCMC 1 cut(s) 482
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 1157
BanII GRGCYC 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 1178
BbvI GCAGC 7 cut(s) 84, 135, 334, 387, 710, 996, 1156
BccI CCATC 2 cut(s) 1103, 1207
BceAI ACGGC 4 cut(s) 136, 454, 591, 621
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 1 cut(s) 22
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 3 cut(s) 293, 410, 1030
BcoDI GTCTC 2 cut(s) 344, 1024
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 22
BglI GCCNNNNNGGC 3 cut(s) 486, 744, 1180
Bme1390I CCNGG 4 cut(s) 293, 327, 410, 1030
Bme18I GGWCC 3 cut(s) 433, 940, 1032
BmgT120I GGNCC 6 cut(s) 433, 478, 479, 940, 1032, 1037
BmrFI CCNGG 4 cut(s) 293, 327, 410, 1030
BmsI GCATC 3 cut(s) 228, 456, 587
Bpu10I CCTNAGC 1 cut(s) 1178
BpuMI CCSGG 3 cut(s) 293, 410, 1030
BsaBI GATNNNNATC 1 cut(s) 1094
BsaHI GRCGYC 1 cut(s) 608
BsaJI CCNNGG 4 cut(s) 292, 326, 786, 1111
BsaWI WCCGGW 1 cut(s) 937
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 488
Bse118I RCCGGY 6 cut(s) 430, 637, 772, 859, 1034, 1129
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 1 cut(s) 994
Bse8I GATNNNNATC 1 cut(s) 1094
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 4 cut(s) 292, 326, 786, 1111
BseJI GATNNNNATC 1 cut(s) 1094
BseLI CCNNNNNNNGG 2 cut(s) 476, 488
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 2 cut(s) 1077, 1169
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 4 cut(s) 47, 50, 293, 894
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 7 cut(s) 84, 135, 334, 387, 710, 996, 1156
BseYI CCCAGC 2 cut(s) 474, 1170
BsgI GTGCAG 1 cut(s) 1163
Bsh1236I CGCG 2 cut(s) 167, 854
Bsh1285I CGRYCG 2 cut(s) 66, 1029
BshFI GGCC 5 cut(s) 85, 104, 480, 747, 1038
BshNI GGYRCC 1 cut(s) 1157
BsiEI CGRYCG 2 cut(s) 66, 1029
BsiHKAI GWGCWC 1 cut(s) 141
BslFI GGGAC 3 cut(s) 139, 309, 1045
BslI CCNNNNNNNGG 2 cut(s) 476, 488
BsmAI GTCTC 2 cut(s) 344, 1024
BsmBI CGTCTC 2 cut(s) 344, 1024
BsmFI GGGAC 3 cut(s) 139, 309, 1045
BsmI GAATGC 1 cut(s) 1121
BsnI GGCC 5 cut(s) 85, 104, 480, 747, 1038
Bsp120I GGGCCC 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 141, 482
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
Bsp19I CCATGG 1 cut(s) 1111
BspANI GGCC 5 cut(s) 85, 104, 480, 747, 1038
BspCNI CTCAG 2 cut(s) 1078, 1170
BspFNI CGCG 2 cut(s) 167, 854
BspHI TCATGA 1 cut(s) 706
BspPI GGATC 4 cut(s) 212, 225, 571, 1189
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 1157
BsrDI GCAATG 1 cut(s) 994
BsrFI RCCGGY 6 cut(s) 430, 637, 772, 859, 1034, 1129
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 1 cut(s) 659
BssAI RCCGGY 6 cut(s) 430, 637, 772, 859, 1034, 1129
BssECI CCNNGG 4 cut(s) 292, 326, 786, 1111
BssMI GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
BssNI GRCGYC 1 cut(s) 608
BssT1I CCWWGG 2 cut(s) 786, 1111
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 5 cut(s) 192, 305, 679, 1054, 1192
Bst6I CTCTTC 2 cut(s) 260, 312
BstACI GRCGYC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 5 cut(s) 102, 165, 243, 1131, 1149
BstDEI CTNAG 2 cut(s) 1086, 1178
BstDSI CCRYGG 1 cut(s) 1111
BstFNI CGCG 2 cut(s) 167, 854
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 5 cut(s) 169, 209, 405, 517, 698
BstKTI GATC 6 cut(s) 66, 220, 369, 566, 1092, 1197
BstMAI GTCTC 2 cut(s) 344, 1024
BstMBI GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
BstMCI CGRYCG 2 cut(s) 66, 1029
BstMWI GCNNNNNNNGC 9 cut(s) 145, 229, 238, 486, 729, 744, 860, 981, 1180
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 1151
BstSCI CCNGG 4 cut(s) 291, 325, 408, 1028
BstSLI GKGCMC 1 cut(s) 482
BstUI CGCG 2 cut(s) 167, 854
BstV1I GCAGC 7 cut(s) 84, 135, 334, 387, 710, 996, 1156
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 5 cut(s) 85, 104, 480, 747, 1038
BtgI CCRYGG 1 cut(s) 1111
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 4 cut(s) 666, 730, 917, 1188
Cac8I GCNNGC 5 cut(s) 102, 165, 243, 1131, 1149
CaiI CAGNNNCTG 1 cut(s) 1177
CciI TCATGA 1 cut(s) 706
CfoI GCGC 5 cut(s) 169, 209, 405, 517, 698
Cfr10I RCCGGY 6 cut(s) 430, 637, 772, 859, 1034, 1129
Cfr13I GGNCC 6 cut(s) 433, 478, 479, 940, 1032, 1037
CseI GACGC 1 cut(s) 597
Csp6I GTAC 4 cut(s) 418, 424, 448, 1069
CviAII CATG 4 cut(s) 707, 1058, 1112, 1148
CviQI GTAC 4 cut(s) 418, 424, 448, 1069
DdeI CTNAG 2 cut(s) 1086, 1178
DpnI GATC 6 cut(s) 65, 219, 368, 565, 1091, 1196
DpnII GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 260, 312
EarI CTCTTC 2 cut(s) 260, 312
Eco130I CCWWGG 2 cut(s) 786, 1111
Eco24I GRGCYC 1 cut(s) 482
Eco47I GGWCC 3 cut(s) 433, 940, 1032
Eco47III AGCGCT 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 941
EcoO109I RGGNCCY 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 1136
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 786, 1111
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 2 cut(s) 786, 1111
Esp3I CGTCTC 2 cut(s) 344, 1024
FaeI CATG 4 cut(s) 710, 1061, 1115, 1151
FaiI YATR 7 cut(s) 519, 708, 755, 767, 1059, 1113, 1149
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FaqI GGGAC 3 cut(s) 139, 309, 1045
FatI CATG 4 cut(s) 706, 1057, 1111, 1147
FauI CCCGC 4 cut(s) 225, 267, 435, 485
FbaI TGATCA 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 482
GlaI GCGC 5 cut(s) 168, 208, 404, 516, 697
GsaI CCCAGC 2 cut(s) 478, 1174
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 5 cut(s) 85, 104, 480, 747, 1038
HgaI GACGC 1 cut(s) 597
HhaI GCGC 5 cut(s) 169, 209, 405, 517, 698
Hin1I GRCGYC 1 cut(s) 608
Hin1II CATG 4 cut(s) 710, 1061, 1115, 1151
Hin6I GCGC 5 cut(s) 167, 207, 403, 515, 696
HinP1I GCGC 5 cut(s) 167, 207, 403, 515, 696
HinfI GANTC 4 cut(s) 454, 791, 823, 896
HphI GGTGA 4 cut(s) 343, 648, 876, 1084
Hpy166II GTNNAC 2 cut(s) 450, 645
Hpy188I TCNGA 7 cut(s) 126, 196, 375, 685, 847, 960, 1087
Hpy188III TCNNGA 4 cut(s) 21, 215, 707, 1021
Hpy8I GTNNAC 2 cut(s) 450, 645
Hpy99I CGWCG 3 cut(s) 181, 613, 1027
HpyAV CCTTC 7 cut(s) 75, 180, 246, 541, 704, 916, 1010
HpyCH4III ACNGT 5 cut(s) 192, 305, 679, 1054, 1192
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 2 cut(s) 241, 1144
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 229, 238, 486, 729, 744, 860, 981, 1180
HpyF3I CTNAG 2 cut(s) 1086, 1178
HpySE526I ACGT 1 cut(s) 1017
Hsp92I GRCGYC 1 cut(s) 608
Hsp92II CATG 4 cut(s) 710, 1061, 1115, 1151
HspAI GCGC 5 cut(s) 167, 207, 403, 515, 696
KroI GCCGGC 1 cut(s) 1129
KroNI GCCGGC 1 cut(s) 1131
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 4 cut(s) 127, 196, 397, 550
Lsp1109I GCAGC 7 cut(s) 84, 135, 334, 387, 710, 996, 1156
LweI GCATC 3 cut(s) 228, 456, 587
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 1 cut(s) 882
MalI GATC 6 cut(s) 65, 219, 368, 565, 1091, 1196
MboI GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
MboII GAAGA 5 cut(s) 277, 295, 329, 346, 891
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 141, 482
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 5 cut(s) 376, 781, 927, 945, 1136
MluNI TGGCCA 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 1129
MroXI GAANNNNTTC 1 cut(s) 739
MscI TGGCCA 1 cut(s) 104
MslI CAYNNNNRTG 4 cut(s) 661, 711, 1097, 1190
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 1177
MspR9I CCNGG 4 cut(s) 293, 327, 410, 1030
Mva1269I GAATGC 1 cut(s) 1121
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 2 cut(s) 167, 854
MwoI GCNNNNNNNGC 9 cut(s) 145, 229, 238, 486, 729, 744, 860, 981, 1180
NaeI GCCGGC 1 cut(s) 1131
NciI CCSGG 3 cut(s) 293, 410, 1030
NcoI CCATGG 1 cut(s) 1111
NdeII GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
NgoMIV GCCGGC 1 cut(s) 1129
NlaIII CATG 4 cut(s) 710, 1061, 1115, 1151
NmuCI GTSAC 1 cut(s) 882
NspI RCATGY 1 cut(s) 1151
OliI CACNNNNGTG 2 cut(s) 661, 1097
PaeI GCATGC 1 cut(s) 1151
PagI TCATGA 1 cut(s) 706
PciSI GCTCTTC 1 cut(s) 312
PctI GAATGC 1 cut(s) 1121
PdiI GCCGGC 1 cut(s) 1131
PdmI GAANNNNTTC 1 cut(s) 739
PfeI GAWTC 4 cut(s) 454, 791, 823, 896
Ple19I CGATCG 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 325
PspFI CCCAGC 2 cut(s) 474, 1170
PspGI CCWGG 1 cut(s) 325
PspOMI GGGCCC 1 cut(s) 478
PspPI GGNCC 6 cut(s) 433, 478, 479, 940, 1032, 1037
PstNI CAGNNNCTG 1 cut(s) 1177
PsuI RGATCY 1 cut(s) 217
PvuI CGATCG 1 cut(s) 66
RsaI GTAC 4 cut(s) 419, 425, 449, 1070
RsaNI GTAC 4 cut(s) 418, 424, 448, 1069
RseI CAYNNNNRTG 4 cut(s) 661, 711, 1097, 1190
SapI GCTCTTC 1 cut(s) 312
Sau3AI GATC 6 cut(s) 63, 217, 366, 563, 1089, 1194
Sau96I GGNCC 6 cut(s) 433, 478, 479, 940, 1032, 1037
ScrFI CCNGG 4 cut(s) 293, 327, 410, 1030
SduI GDGCHC 2 cut(s) 141, 482
SetI ASST 9 cut(s) 12, 48, 77, 102, 124, 402, 703, 715, 1020
SfaNI GCATC 3 cut(s) 228, 456, 587
SinI GGWCC 3 cut(s) 433, 940, 1032
SmiMI CAYNNNNRTG 4 cut(s) 661, 711, 1097, 1190
SphI GCATGC 1 cut(s) 1151
Sse9I AATT 5 cut(s) 376, 781, 927, 945, 1136
StyD4I CCNGG 4 cut(s) 291, 325, 408, 1028
StyI CCWWGG 2 cut(s) 786, 1111
TaaI ACNGT 5 cut(s) 192, 305, 679, 1054, 1192
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 4 cut(s) 62, 95, 114, 1022
TasI AATT 5 cut(s) 376, 781, 927, 945, 1136
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 4 cut(s) 72, 489, 492, 1177
TfiI GAWTC 4 cut(s) 454, 791, 823, 896
TscAI CASTG 4 cut(s) 666, 730, 924, 1195
TseFI GTSAC 1 cut(s) 882
TseI GCWGC 7 cut(s) 72, 148, 322, 400, 698, 984, 1144
Tsp45I GTSAC 1 cut(s) 882
TspDTI ATGAA 3 cut(s) 723, 825, 1046
TspGWI ACGGA 4 cut(s) 165, 362, 402, 435
TspRI CASTG 4 cut(s) 666, 730, 924, 1195
VpaK11BI GGWCC 3 cut(s) 433, 940, 1032
XapI RAATTY 3 cut(s) 376, 781, 1136
XceI RCATGY 1 cut(s) 1151
XmnI GAANNNNTTC 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.