pycom05g15750

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013546
Physical Location & Seq
Forward (+)
19261261 .. 19262915
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15750.2

Sequence Viewer

Length: 1164 bp
ATGGGAGAGGTATCCAAATCGAGATTGAAAGAGGAGGAGATTGAGCTGGATTTGGGGCTGTCGATCGGCGGCAGCTTTCGAAGGCCGGAGAACTCGAAGCTGGCCAACGGGTTCGATGTAGCTCCAAGAGAGAGCGAGTACCAAACGCTGCCATCCCAACCGCACGTGCCCTCTCCGTCGCCGATCGAACCGTCGGAGCCGGAGAGCGCTCGTCTGGATCCGCAGACGAAGCGAGAGATGCAGGCGTTGAGAAGGCAGGAAGCGAAGAGGAAGCGGGAGGAGAAGCAAATCCGGGGACTGAACGGTTCCCAAGCGGAAGAGCAGCCTGGGGTGAAGAAAGAGAGAACGGAGACGAATGTGAATGTGAATCTGAATTTGAGCAACGGACAAAGGGAGCTGCGCTACCCGGTTCAGTACCCGTACCTGCCGGTCCATTTTGTTCCGTACACGAATCCTTGCGTGATGCCTTGCTGGGGTTTCCGGCCTTTTCAGGCGCATAAGCCGAAGTTGAATAACGGGTGTGAGACGGAGCAGAATGGTGGGTCGGGTAAAACGGCGTCGGTAAATGGTGGGTTGGGTAAGAAACCGGCGCCGACAAATGTTTCACCAATGTGTAGTTCCTCCACCGTGTCAGATCATCAAAGCGCAGCTTCTCATGAAGGTGGTGGAAGCAGTGAGAGCAGAAGCCATTCAAGCCAGAGCATACAGGAAAACATAGAGCCGGTTTCAAATTCCTTGGACTCCAATTCCAAAGGGAATGAACAAAAACTGAATCCAAAAACAGAACCCAAATCAGAACACGCGGAACCGGCTCCAATCCCAAAACCCATCTCACCAAAACAAGAATCTTCTCCTCCATTTCCTCCACGGAAGGAAATTGCCAAAAGGGACCCAATTTCCAAGCCTCCAAAACCCCCGATGAGGAAACATGGCAGCAATACCGTTTCCCTTCCCCAAATGCCTTACGTCTCGACGACCGGGACCGGCCCGAATGGGAAAACGGTTCATGGGTTGTTGTACAGATACACGGAATCGGAGATCACCATCGTGTGCGGCTGCCATGGAACCACATTCTCGCCGGCTGAATTCGTGCAGCATGCCGGAGGCACCGATGTTTCCCAGCCGCTGAGGCACATCACTGTGATCCCATCTGCTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAR Jas

Protein Analysis

388

Amino Acids

42.21

Weight (kDa)

8.76

Isoelectric Point (pI)

69.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013546)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07255
fragaria_vesca FvH4_2g18440 FvH4_2g18440
malus_domestica MD05G1170300.v1.1 MD10G1158500.v1.1
prunus_persica Prupe.8G197500_v2.0.a1
pyrus_communis pycom05g15750
rosa_chinensis RchiOBHm_Chr6g0283921
rosa_laevigata RLG00000012786
rosa_multiflora Rmu_ssc0000442.1_g000021
rosa_roxburghii Rroxscaffold_6G00425120 Rroxscaffold_7G00185060
rosa_rugosa Rorug06G0158500
rosa_samantha Rh6AG271500 Rh6BG272700 Rh6CG273900 Rh6DG266800
rosa_wichuraiana Rw6G023420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 432
AccB1I GGYRCC 2 cut(s) 589, 1106
AccII CGCG 1 cut(s) 803
AciI CCGC 8 cut(s) 69, 161, 221, 274, 314, 803, 1053, 1124
AclWI GGATC 3 cut(s) 212, 225, 1138
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 3 cut(s) 373, 730, 1085
AcvI CACGTG 1 cut(s) 166
AcyI GRCGYC 2 cut(s) 557, 590
AfaI GTAC 5 cut(s) 140, 416, 422, 446, 1019
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 473, 921
AgsI TTSAA 4 cut(s) 28, 511, 693, 729
AjnI CCWGG 1 cut(s) 325
AjuI GAANNNNNNNTTGG 2 cut(s) 601, 633
AleI CACNNNNGTG 2 cut(s) 610, 1046
AluBI AGCT 6 cut(s) 46, 75, 100, 122, 397, 650
AluI AGCT 6 cut(s) 46, 75, 100, 122, 397, 650
Alw26I GTCTC 3 cut(s) 344, 518, 973
AlwI GGATC 3 cut(s) 212, 225, 1138
AlwNI CAGNNNCTG 1 cut(s) 1126
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 4 cut(s) 83, 102, 482, 985
ApeKI GCWGC 8 cut(s) 72, 148, 322, 397, 647, 933, 1056, 1093
ApoI RAATTY 3 cut(s) 373, 730, 1085
Asp700I GAANNNNTTC 1 cut(s) 688
AspLEI GCGC 5 cut(s) 209, 402, 496, 592, 647
AspS9I GGNCC 4 cut(s) 430, 889, 981, 986
AsuC2I CCSGG 3 cut(s) 293, 407, 979
AsuHPI GGTGA 4 cut(s) 343, 597, 825, 1033
AsuII TTCGAA 1 cut(s) 79
AvaII GGWCC 3 cut(s) 430, 889, 981
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 589, 1106
BbrPI CACGTG 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 1127
BbvI GCAGC 8 cut(s) 84, 135, 334, 384, 659, 945, 1043, 1105
BccI CCATC 4 cut(s) 160, 836, 1052, 1156
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 1 cut(s) 22
BcnI CCSGG 3 cut(s) 293, 407, 979
BcoDI GTCTC 3 cut(s) 344, 518, 973
BfoI RGCGCY 2 cut(s) 210, 593
BfuAI ACCTGC 1 cut(s) 432
BfuI GTATCC 1 cut(s) 22
BglI GCCNNNNNGGC 1 cut(s) 1129
Bme1390I CCNGG 4 cut(s) 293, 327, 407, 979
Bme18I GGWCC 3 cut(s) 430, 889, 981
BmgT120I GGNCC 4 cut(s) 430, 889, 981, 986
BmrFI CCNGG 4 cut(s) 293, 327, 407, 979
BmsI GCATC 2 cut(s) 228, 453
Bpu10I CCTNAGC 1 cut(s) 1127
Bpu14I TTCGAA 1 cut(s) 79
BpuMI CCSGG 3 cut(s) 293, 407, 979
BsaAI YACGTR 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 1043
BsaHI GRCGYC 2 cut(s) 557, 590
BsaJI CCNNGG 5 cut(s) 292, 326, 735, 866, 1060
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 921
Bse118I RCCGGY 6 cut(s) 427, 586, 721, 808, 983, 1078
Bse8I GATNNNNATC 1 cut(s) 1043
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 5 cut(s) 292, 326, 735, 866, 1060
BseGI GGATG 1 cut(s) 152
BseJI GATNNNNATC 1 cut(s) 1043
BseLI CCNNNNNNNGG 2 cut(s) 473, 921
BseMII CTCAG 1 cut(s) 1118
BseRI GAGGAG 4 cut(s) 47, 50, 293, 843
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 8 cut(s) 84, 135, 334, 384, 659, 945, 1043, 1105
BseYI CCCAGC 2 cut(s) 471, 1119
BsgI GTGCAG 1 cut(s) 1112
Bsh1236I CGCG 1 cut(s) 803
Bsh1285I CGRYCG 3 cut(s) 66, 186, 978
BshFI GGCC 4 cut(s) 85, 104, 484, 987
BshNI GGYRCC 2 cut(s) 589, 1106
BsiEI CGRYCG 3 cut(s) 66, 186, 978
BslFI GGGAC 3 cut(s) 309, 902, 994
BslI CCNNNNNNNGG 2 cut(s) 473, 921
BsmAI GTCTC 3 cut(s) 344, 518, 973
BsmBI CGTCTC 3 cut(s) 344, 518, 973
BsmFI GGGAC 3 cut(s) 309, 902, 994
BsnI GGCC 4 cut(s) 85, 104, 484, 987
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 171
Bsp1407I TGTACA 1 cut(s) 1017
Bsp143I GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
Bsp19I CCATGG 1 cut(s) 1060
BspACI CCGC 8 cut(s) 69, 161, 221, 274, 314, 803, 1053, 1124
BspANI GGCC 4 cut(s) 85, 104, 484, 987
BspCNI CTCAG 1 cut(s) 1119
BspFNI CGCG 1 cut(s) 803
BspHI TCATGA 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 432
BspPI GGATC 3 cut(s) 212, 225, 1138
BspQI GCTCTTC 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 79
BspT107I GGYRCC 2 cut(s) 589, 1106
BsrFI RCCGGY 6 cut(s) 427, 586, 721, 808, 983, 1078
BsrGI TGTACA 1 cut(s) 1017
BssAI RCCGGY 6 cut(s) 427, 586, 721, 808, 983, 1078
BssECI CCNNGG 5 cut(s) 292, 326, 735, 866, 1060
BssMI GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
BssNI GRCGYC 2 cut(s) 557, 590
BssT1I CCWWGG 2 cut(s) 735, 1060
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 6 cut(s) 192, 305, 628, 943, 1003, 1141
Bst6I CTCTTC 2 cut(s) 260, 312
BstACI GRCGYC 2 cut(s) 557, 590
BstAUI TGTACA 1 cut(s) 1017
BstBAI YACGTR 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 4 cut(s) 102, 243, 1080, 1098
BstDEI CTNAG 1 cut(s) 1127
BstDSI CCRYGG 2 cut(s) 866, 1060
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 1 cut(s) 803
BstH2I RGCGCY 2 cut(s) 210, 593
BstHHI GCGC 5 cut(s) 209, 402, 496, 592, 647
BstKTI GATC 6 cut(s) 66, 186, 220, 637, 1041, 1146
BstMAI GTCTC 3 cut(s) 344, 518, 973
BstMBI GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
BstMCI CGRYCG 3 cut(s) 66, 186, 978
BstMWI GCNNNNNNNGC 6 cut(s) 229, 238, 678, 693, 809, 1129
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 1100
BstSCI CCNGG 4 cut(s) 291, 325, 405, 977
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 1 cut(s) 803
BstV1I GCAGC 8 cut(s) 84, 135, 334, 384, 659, 945, 1043, 1105
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 4 cut(s) 85, 104, 484, 987
BtgI CCRYGG 2 cut(s) 866, 1060
BtsCI GGATG 1 cut(s) 152
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 2 cut(s) 679, 1137
BveI ACCTGC 1 cut(s) 432
Cac8I GCNNGC 4 cut(s) 102, 243, 1080, 1098
CaiI CAGNNNCTG 1 cut(s) 1126
CciI TCATGA 1 cut(s) 655
CfoI GCGC 5 cut(s) 209, 402, 496, 592, 647
Cfr10I RCCGGY 6 cut(s) 427, 586, 721, 808, 983, 1078
Cfr13I GGNCC 4 cut(s) 430, 889, 981, 986
CseI GACGC 1 cut(s) 546
Csp6I GTAC 5 cut(s) 139, 415, 421, 445, 1018
CviAII CATG 5 cut(s) 656, 929, 1007, 1061, 1097
CviQI GTAC 5 cut(s) 139, 415, 421, 445, 1018
DdeI CTNAG 1 cut(s) 1127
DinI GGCGCC 1 cut(s) 591
DpnI GATC 6 cut(s) 65, 185, 219, 636, 1040, 1145
DpnII GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 260, 312
EarI CTCTTC 2 cut(s) 260, 312
Eco130I CCWWGG 2 cut(s) 735, 1060
Eco47I GGWCC 3 cut(s) 430, 889, 981
Eco47III AGCGCT 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 889
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 735, 1060
EgeI GGCGCC 1 cut(s) 591
EheI GGCGCC 1 cut(s) 591
ErhI CCWWGG 2 cut(s) 735, 1060
Esp3I CGTCTC 3 cut(s) 344, 518, 973
FaeI CATG 5 cut(s) 659, 932, 1010, 1064, 1100
FaiI YATR 8 cut(s) 498, 657, 704, 716, 930, 1008, 1062, 1098
FalI AAGNNNNNCTT 2 cut(s) 634, 666
FaqI GGGAC 3 cut(s) 309, 902, 994
FatI CATG 5 cut(s) 655, 928, 1006, 1060, 1096
FauI CCCGC 1 cut(s) 267
FokI GGATG 1 cut(s) 139
GlaI GCGC 5 cut(s) 208, 401, 495, 591, 646
GsaI CCCAGC 2 cut(s) 475, 1123
HaeII RGCGCY 2 cut(s) 210, 593
HaeIII GGCC 4 cut(s) 85, 104, 484, 987
HgaI GACGC 1 cut(s) 546
HhaI GCGC 5 cut(s) 209, 402, 496, 592, 647
Hin1I GRCGYC 2 cut(s) 557, 590
Hin1II CATG 5 cut(s) 659, 932, 1010, 1064, 1100
Hin6I GCGC 5 cut(s) 207, 400, 494, 590, 645
HinP1I GCGC 5 cut(s) 207, 400, 494, 590, 645
HinfI GANTC 6 cut(s) 367, 451, 740, 772, 845, 1031
HphI GGTGA 4 cut(s) 343, 597, 825, 1033
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 5 cut(s) 196, 372, 634, 796, 1036
Hpy188III TCNNGA 4 cut(s) 21, 215, 656, 970
Hpy8I GTNNAC 1 cut(s) 447
Hpy99I CGWCG 4 cut(s) 181, 196, 562, 976
HpyAV CCTTC 5 cut(s) 75, 246, 653, 865, 959
HpyCH4III ACNGT 6 cut(s) 192, 305, 628, 943, 1003, 1141
HpyCH4IV ACGT 2 cut(s) 165, 966
HpyCH4V TGCA 2 cut(s) 241, 1093
HpyF10VI GCNNNNNNNGC 6 cut(s) 229, 238, 678, 693, 809, 1129
HpyF3I CTNAG 1 cut(s) 1127
HpySE526I ACGT 2 cut(s) 165, 966
Hsp92I GRCGYC 2 cut(s) 557, 590
Hsp92II CATG 5 cut(s) 659, 932, 1010, 1064, 1100
HspAI GCGC 5 cut(s) 207, 400, 494, 590, 645
KasI GGCGCC 1 cut(s) 589
KflI GGGWCCC 1 cut(s) 889
KroI GCCGGC 1 cut(s) 1078
KroNI GCCGGC 1 cut(s) 1080
Kzo9I GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 5 cut(s) 127, 196, 394, 529, 817
Lsp1109I GCAGC 8 cut(s) 84, 135, 334, 384, 659, 945, 1043, 1105
LweI GCATC 2 cut(s) 228, 453
MaeII ACGT 2 cut(s) 165, 966
MalI GATC 6 cut(s) 65, 185, 219, 636, 1040, 1145
MboI GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
MboII GAAGA 4 cut(s) 277, 329, 346, 840
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 6 cut(s) 373, 730, 745, 876, 894, 1085
MluNI TGGCCA 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 590
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 1078
MroXI GAANNNNTTC 1 cut(s) 688
MscI TGGCCA 1 cut(s) 104
MslI CAYNNNNRTG 4 cut(s) 610, 660, 1046, 1139
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 1126
MspR9I CCNGG 4 cut(s) 293, 327, 407, 979
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 1 cut(s) 803
MwoI GCNNNNNNNGC 6 cut(s) 229, 238, 678, 693, 809, 1129
NaeI GCCGGC 1 cut(s) 1080
NarI GGCGCC 1 cut(s) 590
NciI CCSGG 3 cut(s) 293, 407, 979
NcoI CCATGG 1 cut(s) 1060
NdeII GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
NgoMIV GCCGGC 1 cut(s) 1078
NlaIII CATG 5 cut(s) 659, 932, 1010, 1064, 1100
NspI RCATGY 1 cut(s) 1100
NspV TTCGAA 1 cut(s) 79
OliI CACNNNNGTG 2 cut(s) 610, 1046
PaeI GCATGC 1 cut(s) 1100
PagI TCATGA 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 312
PdiI GCCGGC 1 cut(s) 1080
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 5 cut(s) 367, 451, 772, 845, 1031
Ple19I CGATCG 2 cut(s) 66, 186
PleI GAGTC 1 cut(s) 734
PluTI GGCGCC 1 cut(s) 593
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 1 cut(s) 734
Ppu21I YACGTR 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 889
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 1 cut(s) 325
PspCI CACGTG 1 cut(s) 166
PspFI CCCAGC 2 cut(s) 471, 1119
PspGI CCWGG 1 cut(s) 325
PspPI GGNCC 4 cut(s) 430, 889, 981, 986
PspPPI RGGWCCY 1 cut(s) 889
PstNI CAGNNNCTG 1 cut(s) 1126
PsuI RGATCY 1 cut(s) 217
PvuI CGATCG 2 cut(s) 66, 186
RsaI GTAC 5 cut(s) 140, 416, 422, 446, 1019
RsaNI GTAC 5 cut(s) 139, 415, 421, 445, 1018
RseI CAYNNNNRTG 4 cut(s) 610, 660, 1046, 1139
SapI GCTCTTC 1 cut(s) 312
Sau3AI GATC 6 cut(s) 63, 183, 217, 634, 1038, 1143
Sau96I GGNCC 4 cut(s) 430, 889, 981, 986
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 4 cut(s) 293, 327, 407, 979
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 2 cut(s) 228, 453
SfoI GGCGCC 1 cut(s) 591
SfuI TTCGAA 1 cut(s) 79
SinI GGWCC 3 cut(s) 430, 889, 981
SmiMI CAYNNNNRTG 4 cut(s) 610, 660, 1046, 1139
SphI GCATGC 1 cut(s) 1100
Sse9I AATT 6 cut(s) 373, 730, 745, 876, 894, 1085
SsiI CCGC 8 cut(s) 69, 161, 221, 274, 314, 803, 1053, 1124
SspDI GGCGCC 1 cut(s) 589
StyD4I CCNGG 4 cut(s) 291, 325, 405, 977
StyI CCWWGG 2 cut(s) 735, 1060
TaaI ACNGT 6 cut(s) 192, 305, 628, 943, 1003, 1141
TaiI ACGT 2 cut(s) 168, 969
TaqI TCGA 7 cut(s) 20, 62, 79, 95, 114, 186, 971
TasI AATT 6 cut(s) 373, 730, 745, 876, 894, 1085
TatI WGTACW 1 cut(s) 1017
TauI GCSGC 3 cut(s) 72, 1056, 1126
TfiI GAWTC 5 cut(s) 367, 451, 772, 845, 1031
TscAI CASTG 2 cut(s) 679, 1144
TseI GCWGC 8 cut(s) 72, 148, 322, 397, 647, 933, 1056, 1093
TspDTI ATGAA 3 cut(s) 672, 774, 995
TspGWI ACGGA 7 cut(s) 165, 362, 399, 432, 542, 883, 1043
TspRI CASTG 2 cut(s) 679, 1144
VpaK11BI GGWCC 3 cut(s) 430, 889, 981
XapI RAATTY 3 cut(s) 373, 730, 1085
XceI RCATGY 1 cut(s) 1100
XmnI GAANNNNTTC 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.