MD10G1158500.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013546
Physical Location & Seq
Forward (+)
24824926 .. 24826152
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1158500.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGGTGGAGGTTTCCGAATCGGCATCGAAAGAGGAGGAGGTTGAGCTGGATTTGGGGCTGTCGATCGGCGGCAGCTTCCGAAAACCGGAGAACTCGAAGCTGGCCAAAGGGTTCGACGTAGATCCGAGAAAGACTGAGCACCAAATACAGCCATCCCAACCGAACGCGCCATCTCCATCGCCGTTCGAGTCGTCGGAGCCGGAGAGCGCGCTTCTGGATCCGCATACGAAACGGGAGATGCAGGCGTTGAGAAGGCAGGAAGCGAAGAGGAAGCGGGAGCAGAAGAAAGGCCGGGGACAGAACGATCCCCAAGTGGAGGAACACCCGGCGGTGAAGAAAGAGAGAACGGAGACGAATGTGAATGTGAATGTGAATCTGAATTTGAGCAACGGACAAACGGAGCTGCGCTGCCCGGTTCAACAACCAGGTCAGTACCCGTACCCGCCGGTTCAGTTTGTTCCGTACATGAACGGGTTCTCGTATCCTTGCGTGATGCCGTGCTGGGCCCCCAGCGGCGGCGGCGGGTTCCGGCCCTTTCAGGCGCATAAGGATACGGGGAAGAAGAAGAACGGGTGTGAGACGGAGCAGAATAGTGGGTCGGGTAAAACGGCGCCGGTGAATGGTGGGTTGGGTAAGAAACCGGCGTCGGTAAATGTTTCACCAATGTGTAGTTCCTCCACCGTGTCAGATCATCAAAGCGCAGCTTCTCAAAACGGTATGTTGGGTGGTGGAAGCAGCGAGAGCAAAAGCCATTCAAGCCAGAGCATACAAGAAAACACAGAGCCGGTTTCAAATTCCTTGGAATCCAATTCCGAAGGGAATGAACAAAAACTGAATCCAAAAACAGAACCGAAATCAGAAACCGCAGAAGCATTTCCAACCCCAAAACCCATCTCACCTAAACAAGAATATTCCCCTCCAATCCCTCCACTGAAGGAAGTTACCAAAACGGACCCAATTGCCAAGCCTCCAAAACCCCAGATCAGCAAAAATGGCAGCAGCACCGTTTCCCTTCCCCAAATGCCTTATGTCTCGACGACCGGGACCGGGCCTAATGGGAAAACAGTTCATGGGTTCTTGGACAGATACACGAATTCGGAGATTACCATCGTGTGCGTCTGCCATGGAACCACATTCTCGCCGTCTGAATTCGTGCAGCATGCCGGAGGCACCGATGTTTCGCAGCCGCTGAGGCACATAACGGTGATCCCTTCTGCTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAR Jas

Protein Analysis

409

Amino Acids

43.94

Weight (kDa)

7.14

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAR PF07897 11 - 33 3.3e-06 Ethylene-responsive binding factor-associated repression
TDBD PF16135 369 - 401 8.9e-09 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013546)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07255
fragaria_vesca FvH4_2g18440 FvH4_2g18440
malus_domestica MD05G1170300.v1.1 MD10G1158500.v1.1
prunus_persica Prupe.8G197500_v2.0.a1
pyrus_communis pycom05g15750
rosa_chinensis RchiOBHm_Chr6g0283921
rosa_laevigata RLG00000012786
rosa_multiflora Rmu_ssc0000442.1_g000021
rosa_roxburghii Rroxscaffold_6G00425120 Rroxscaffold_7G00185060
rosa_rugosa Rorug06G0158500
rosa_samantha Rh6AG271500 Rh6BG272700 Rh6CG273900 Rh6DG266800
rosa_wichuraiana Rw6G023420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 610, 1169
AccII CGCG 2 cut(s) 167, 209
AclWI GGATC 5 cut(s) 116, 212, 225, 299, 1201
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 4 cut(s) 379, 793, 1093, 1148
AcuI CTGAAG 1 cut(s) 953
AcyI GRCGYC 2 cut(s) 611, 644
AfaI GTAC 3 cut(s) 434, 440, 464
AfiI CCNNNNNNNGG 5 cut(s) 85, 316, 515, 620, 1047
AgsI TTSAA 3 cut(s) 419, 756, 792
AjnI CCWGG 1 cut(s) 424
AjuI GAANNNNNNNTTGG 4 cut(s) 611, 643, 655, 687
AleI CACNNNNGTG 1 cut(s) 664
AluBI AGCT 5 cut(s) 46, 75, 100, 403, 704
AluI AGCT 5 cut(s) 46, 75, 100, 403, 704
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 3 cut(s) 344, 572, 1036
AlwI GGATC 5 cut(s) 116, 212, 225, 299, 1201
AlwNI CAGNNNCTG 1 cut(s) 1189
AoxI GGCC 5 cut(s) 102, 289, 504, 530, 1049
ApaI GGGCCC 1 cut(s) 508
ApeKI GCWGC 9 cut(s) 72, 403, 408, 701, 735, 996, 999, 1156, 1183
ApoI RAATTY 4 cut(s) 379, 793, 1093, 1148
Asp700I GAANNNNTTC 2 cut(s) 473, 873
AspLEI GCGC 7 cut(s) 169, 209, 211, 408, 544, 613, 701
AspS9I GGNCC 6 cut(s) 504, 505, 531, 952, 1044, 1049
AsuC2I CCSGG 5 cut(s) 293, 326, 413, 1042, 1048
AsuHPI GGTGA 5 cut(s) 343, 628, 651, 888, 1216
AvaII GGWCC 2 cut(s) 952, 1044
BaeGI GKGCMC 1 cut(s) 508
BalI TGGCCA 1 cut(s) 104
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 610, 1169
BanII GRGCYC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 1190
BbvI GCAGC 9 cut(s) 84, 390, 395, 713, 747, 1008, 1011, 1168, 1195
BccI CCATC 5 cut(s) 160, 178, 184, 899, 1115
BceAI ACGGC 4 cut(s) 166, 481, 624, 1126
BciT130I CCWGG 1 cut(s) 426
BciVI GTATCC 2 cut(s) 492, 544
BcnI CCSGG 5 cut(s) 293, 326, 413, 1042, 1048
BcoDI GTCTC 3 cut(s) 344, 572, 1036
BfoI RGCGCY 1 cut(s) 614
BfuI GTATCC 2 cut(s) 492, 544
BglI GCCNNNNNGGC 1 cut(s) 1192
Bme1390I CCNGG 6 cut(s) 293, 326, 413, 426, 1042, 1048
Bme18I GGWCC 2 cut(s) 952, 1044
BmgT120I GGNCC 6 cut(s) 504, 505, 531, 952, 1044, 1049
BmrFI CCNGG 6 cut(s) 293, 326, 413, 426, 1042, 1048
BmsI GCATC 3 cut(s) 32, 228, 483
Bpu10I CCTNAGC 1 cut(s) 1190
BpuMI CCSGG 5 cut(s) 293, 326, 413, 1042, 1048
BsaBI GATNNNNATC 1 cut(s) 1106
BsaHI GRCGYC 2 cut(s) 611, 644
BsaJI CCNNGG 3 cut(s) 292, 798, 1123
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 316, 515, 620, 1047
Bse118I RCCGGY 4 cut(s) 445, 613, 640, 784
Bse8I GATNNNNATC 1 cut(s) 1106
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 292, 798, 1123
BseGI GGATG 1 cut(s) 152
BseJI GATNNNNATC 1 cut(s) 1106
BseLI CCNNNNNNNGG 5 cut(s) 85, 316, 515, 620, 1047
BseMII CTCAG 2 cut(s) 126, 1181
BsePI GCGCGC 1 cut(s) 207
BseRI GAGGAG 2 cut(s) 47, 50
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 9 cut(s) 84, 390, 395, 713, 747, 1008, 1011, 1168, 1195
BseYI CCCAGC 2 cut(s) 501, 509
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 2 cut(s) 167, 209
Bsh1285I CGRYCG 2 cut(s) 66, 1041
BshFI GGCC 5 cut(s) 104, 291, 506, 532, 1051
BshNI GGYRCC 2 cut(s) 610, 1169
BsiEI CGRYCG 2 cut(s) 66, 1041
BsiHKAI GWGCWC 1 cut(s) 141
BslFI GGGAC 2 cut(s) 309, 1057
BslI CCNNNNNNNGG 5 cut(s) 85, 316, 515, 620, 1047
BsmAI GTCTC 3 cut(s) 344, 572, 1036
BsmBI CGTCTC 2 cut(s) 344, 572
BsmFI GGGAC 2 cut(s) 309, 1057
BsnI GGCC 5 cut(s) 104, 291, 506, 532, 1051
Bsp120I GGGCCC 1 cut(s) 504
Bsp1286I GDGCHC 2 cut(s) 141, 508
Bsp143I GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
Bsp19I CCATGG 1 cut(s) 1123
BspANI GGCC 5 cut(s) 104, 291, 506, 532, 1051
BspCNI CTCAG 2 cut(s) 127, 1182
BspFNI CGCG 2 cut(s) 167, 209
BspPI GGATC 5 cut(s) 116, 212, 225, 299, 1201
BspT107I GGYRCC 2 cut(s) 610, 1169
BsrFI RCCGGY 4 cut(s) 445, 613, 640, 784
BssAI RCCGGY 4 cut(s) 445, 613, 640, 784
BssECI CCNNGG 3 cut(s) 292, 798, 1123
BssHII GCGCGC 1 cut(s) 207
BssMI GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
BssNI GRCGYC 2 cut(s) 611, 644
BssT1I CCWWGG 2 cut(s) 798, 1123
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 5 cut(s) 682, 716, 1006, 1066, 1204
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 2 cut(s) 611, 644
BstC8I GCNNGC 4 cut(s) 102, 209, 243, 1161
BstDEI CTNAG 2 cut(s) 135, 1190
BstDSI CCRYGG 1 cut(s) 1123
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 2 cut(s) 167, 209
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 7 cut(s) 169, 209, 211, 408, 544, 613, 701
BstKTI GATC 7 cut(s) 66, 124, 220, 307, 691, 984, 1209
BstMAI GTCTC 3 cut(s) 344, 572, 1036
BstMBI GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
BstMCI CGRYCG 2 cut(s) 66, 1041
BstMWI GCNNNNNNNGC 5 cut(s) 519, 741, 756, 993, 1192
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 1163
BstSCI CCNGG 6 cut(s) 291, 324, 411, 424, 1040, 1046
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 2 cut(s) 167, 209
BstV1I GCAGC 9 cut(s) 84, 390, 395, 713, 747, 1008, 1011, 1168, 1195
BstX2I RGATCY 2 cut(s) 121, 217
BstYI RGATCY 2 cut(s) 121, 217
BsuI GTATCC 2 cut(s) 492, 544
BsuRI GGCC 5 cut(s) 104, 291, 506, 532, 1051
BtgI CCRYGG 1 cut(s) 1123
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 929
Cac8I GCNNGC 4 cut(s) 102, 209, 243, 1161
CaiI CAGNNNCTG 1 cut(s) 1189
CfoI GCGC 7 cut(s) 169, 209, 211, 408, 544, 613, 701
Cfr10I RCCGGY 4 cut(s) 445, 613, 640, 784
Cfr13I GGNCC 6 cut(s) 504, 505, 531, 952, 1044, 1049
CseI GACGC 2 cut(s) 633, 1105
CsiI ACCWGGT 1 cut(s) 424
Csp6I GTAC 3 cut(s) 433, 439, 463
CviAII CATG 4 cut(s) 466, 1070, 1124, 1160
CviQI GTAC 3 cut(s) 433, 439, 463
DdeI CTNAG 2 cut(s) 135, 1190
DinI GGCGCC 1 cut(s) 612
DpnI GATC 7 cut(s) 65, 123, 219, 306, 690, 983, 1208
DpnII GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 798, 1123
Eco24I GRGCYC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 952, 1044
Eco57I CTGAAG 1 cut(s) 953
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 2 cut(s) 1093, 1148
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 798, 1123
EcoT38I GRGCYC 1 cut(s) 508
EgeI GGCGCC 1 cut(s) 612
EheI GGCGCC 1 cut(s) 612
ErhI CCWWGG 2 cut(s) 798, 1123
Esp3I CGTCTC 2 cut(s) 344, 572
FaeI CATG 4 cut(s) 469, 1073, 1127, 1163
FalI AAGNNNNNCTT 2 cut(s) 688, 720
FaqI GGGAC 2 cut(s) 309, 1057
FatI CATG 4 cut(s) 465, 1069, 1123, 1159
FauI CCCGC 3 cut(s) 267, 450, 515
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 508
GlaI GCGC 7 cut(s) 168, 208, 210, 407, 543, 612, 700
GsaI CCCAGC 2 cut(s) 505, 513
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 5 cut(s) 104, 291, 506, 532, 1051
HgaI GACGC 2 cut(s) 633, 1105
HhaI GCGC 7 cut(s) 169, 209, 211, 408, 544, 613, 701
Hin1I GRCGYC 2 cut(s) 611, 644
Hin1II CATG 4 cut(s) 469, 1073, 1127, 1163
Hin6I GCGC 7 cut(s) 167, 207, 209, 406, 542, 611, 699
HinP1I GCGC 7 cut(s) 167, 207, 209, 406, 542, 611, 699
HinfI GANTC 5 cut(s) 17, 188, 373, 803, 835
HphI GGTGA 5 cut(s) 343, 628, 651, 888, 1216
Hpy188III TCNNGA 2 cut(s) 215, 1033
Hpy99I CGWCG 4 cut(s) 119, 196, 649, 1039
HpyAV CCTTC 5 cut(s) 246, 809, 928, 1022, 1221
HpyCH4III ACNGT 5 cut(s) 682, 716, 1006, 1066, 1204
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 241, 1156
HpyF10VI GCNNNNNNNGC 5 cut(s) 519, 741, 756, 993, 1192
HpyF3I CTNAG 2 cut(s) 135, 1190
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 2 cut(s) 611, 644
Hsp92II CATG 4 cut(s) 469, 1073, 1127, 1163
HspAI GCGC 7 cut(s) 167, 207, 209, 406, 542, 611, 699
KasI GGCGCC 1 cut(s) 610
Kzo9I GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
LmnI GCTCC 4 cut(s) 196, 277, 400, 583
Lsp1109I GCAGC 9 cut(s) 84, 390, 395, 713, 747, 1008, 1011, 1168, 1195
LweI GCATC 3 cut(s) 32, 228, 483
MabI ACCWGGT 1 cut(s) 424
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 940
MalI GATC 7 cut(s) 65, 123, 219, 306, 690, 983, 1208
MboI GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
MboII GAAGA 6 cut(s) 277, 295, 346, 571, 574, 577
MfeI CAATTG 1 cut(s) 957
MflI RGATCY 2 cut(s) 121, 217
MhlI GDGCHC 2 cut(s) 141, 508
MlsI TGGCCA 1 cut(s) 104
MluCI AATT 6 cut(s) 379, 793, 808, 957, 1093, 1148
MluNI TGGCCA 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 611
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 174, 902
Mox20I TGGCCA 1 cut(s) 104
MroXI GAANNNNTTC 2 cut(s) 473, 873
MscI TGGCCA 1 cut(s) 104
MslI CAYNNNNRTG 2 cut(s) 664, 1202
Msp20I TGGCCA 1 cut(s) 104
MspA1I CMGCKG 2 cut(s) 513, 1189
MspR9I CCNGG 6 cut(s) 293, 326, 413, 426, 1042, 1048
MunI CAATTG 1 cut(s) 957
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 2 cut(s) 167, 209
MwoI GCNNNNNNNGC 5 cut(s) 519, 741, 756, 993, 1192
NarI GGCGCC 1 cut(s) 611
NciI CCSGG 5 cut(s) 293, 326, 413, 1042, 1048
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
NlaIII CATG 4 cut(s) 469, 1073, 1127, 1163
NspI RCATGY 1 cut(s) 1163
OliI CACNNNNGTG 1 cut(s) 664
PaeI GCATGC 1 cut(s) 1163
PauI GCGCGC 1 cut(s) 207
PdmI GAANNNNTTC 2 cut(s) 473, 873
PfeI GAWTC 4 cut(s) 17, 373, 803, 835
Ple19I CGATCG 1 cut(s) 66
PleI GAGTC 1 cut(s) 196
PluTI GGCGCC 1 cut(s) 614
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 424
PspFI CCCAGC 2 cut(s) 501, 509
PspGI CCWGG 1 cut(s) 424
PspOMI GGGCCC 1 cut(s) 504
PspPI GGNCC 6 cut(s) 504, 505, 531, 952, 1044, 1049
PstNI CAGNNNCTG 1 cut(s) 1189
PsuI RGATCY 2 cut(s) 121, 217
PteI GCGCGC 1 cut(s) 207
PvuI CGATCG 1 cut(s) 66
RsaI GTAC 3 cut(s) 434, 440, 464
RsaNI GTAC 3 cut(s) 433, 439, 463
RseI CAYNNNNRTG 2 cut(s) 664, 1202
Sau3AI GATC 7 cut(s) 63, 121, 217, 304, 688, 981, 1206
Sau96I GGNCC 6 cut(s) 504, 505, 531, 952, 1044, 1049
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 6 cut(s) 293, 326, 413, 426, 1042, 1048
SduI GDGCHC 2 cut(s) 141, 508
SexAI ACCWGGT 1 cut(s) 424
SfaNI GCATC 3 cut(s) 32, 228, 483
SfoI GGCGCC 1 cut(s) 612
SgrAI CRCCGGYG 1 cut(s) 613
SinI GGWCC 2 cut(s) 952, 1044
SmiMI CAYNNNNRTG 2 cut(s) 664, 1202
SphI GCATGC 1 cut(s) 1163
Sse9I AATT 6 cut(s) 379, 793, 808, 957, 1093, 1148
SspDI GGCGCC 1 cut(s) 610
SspI AATATT 1 cut(s) 911
StyD4I CCNGG 6 cut(s) 291, 324, 411, 424, 1040, 1046
StyI CCWWGG 2 cut(s) 798, 1123
TaaI ACNGT 5 cut(s) 682, 716, 1006, 1066, 1204
TaiI ACGT 1 cut(s) 120
TaqI TCGA 6 cut(s) 26, 62, 95, 114, 186, 1034
TasI AATT 6 cut(s) 379, 793, 808, 957, 1093, 1148
TauI GCSGC 5 cut(s) 72, 516, 519, 522, 1189
TfiI GAWTC 4 cut(s) 17, 373, 803, 835
TscAI CASTG 1 cut(s) 936
TseI GCWGC 9 cut(s) 72, 403, 408, 701, 735, 996, 999, 1156, 1183
TspDTI ATGAA 3 cut(s) 482, 837, 1058
TspGWI ACGGA 6 cut(s) 362, 405, 413, 450, 596, 965
TspRI CASTG 1 cut(s) 936
VpaK11BI GGWCC 2 cut(s) 952, 1044
XapI RAATTY 4 cut(s) 379, 793, 1093, 1148
XceI RCATGY 1 cut(s) 1163
XmnI GAANNNNTTC 2 cut(s) 473, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.