Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006975

Physical Location & Seq

Genomic Coordinates Reverse (-)

31865005 .. 31869815

Gene Structure 4811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1189900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGGATGATTTCATTTCTCCATTCCACAAAATTATTAACACCATATTAGCTTTGCTTCTGGTTCTCTGTTGTGCATTGAGAATAATCGCAGCATACACTAAGCAAAACAAGAAGAAGAATATCATCAACAACACAAAGACAGAGACCAGATCGTTGACCAAAGTCCCTCAACCTCCAGGAGCATGGCCTTTCATAGGTCACCTTCATCTCCTACATGGCAAAGATCCAGTTGCCCTAATTCTTGGCGCCATGGCCGATAAATTCGGTCCGATCTACTCACTTAAGCTTGGTCCGCGCCAAGTAATGGTGGTGCTTAGCGCTTGGGAACAAGTAAAAGAACGCTTCACAAAAAACGATATTGCTTTCTCCACACGACCGAGTACAGCAGCCGGCAAACACTTGGGTTACAACAACGCTATTTTTGCCCTAGCCCCGTACGGACCCTACTGGCGCGACATCCGGAAGTTGGCTACTCAGGAGTATCTCTCAGCCCAAAAGGTTGAAATGCATAGTCACGTGCGAGCCGAAGAAGTCGACTCTTTCATAAAAAAATTGTTCGATGTGGTAGGGTCGTCAGGGCTCACCACATCGCCCCCCACTTCACCTACTGTAGTGGTGCATTTGAGCGAGTTAGTAGAGCACTTGACGTTTAACATTAGCGTGAGGCTGATTGCCGGGAAGAGATTTTCAGATGGCGAATACAACGAGAAAGGCAGTGAGGCTTGGCGTTTCGAAAAGGCTATGCAGGAGGCTATGCATCTCCTTGGCAATTTTGTTTGGTCGGATGCTATGCCATATTTTGAATGGCTGGACAGCTTGTTTGGGCCTGTGAGTTTCATGAAACGCACATTTAAGGAGCTTGACTCTGTGCTTAGCAGCTGGGTCGAAAGTGACTTGATAGATGCGATGATATCCAACTTTGGAGAGGAGGCTGTCATTTCTGGGCATAGCTGTGATAATGTTATCAAAGCAACATCACTTATTCTCATCATAGGTGGCACACAAAGCACTTCTGTTGCACTAACATGGGCACTTTCCTTACTCCTCAACAACCCAAGTGCCCTAAAATCTGCCCAACAAGAATTGGACATCAACGTAGGAAGTGACAGATGGGTTCAAGAATCCGACCTTCCGAACCTCAAATACCTCCAAGCCATTGTCAAAGAAACCCTACGCTTGTACCCGTCTGTCCCAATCACCGTCCACGAGGGCACGGAGGATTCTCATCTCTCTGGCTATCATGTCCCCAAAGGCACCCGTATAGTGGTCAACTTATGGAAATTGCACCGGGACCCAAGCGTGTGGGCCAACCCTTGTGAGTTTCAACCTGAGAGGTTCATGACCGACCATGCTGACGTGGACTATTTCAAGGGTTTTGAGTACATTCCGTTTAGCTCGGGCAGAAGGTCCTGCCCTGGTGCGACACTAGGGTTGCATGTGGTTCAGTTGCTTCTGGCTCGTTTGCTTCAGGGGTTTGATATGAGCAGAGTCGGTGATGGGCCGGTGGATATGCGTGAAAGGGTGGGTCTTGCCCTAACGAAAGCGGATCCACTTAAGGCCTTTCTTGCTCCACGCCTTCCTCTTCACCTGTATCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0006629 GO:0006720 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0010039 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0042214 GO:0042221 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044550 GO:0046246 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071704 GO:0097007 GO:0097008 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

p450

Protein Analysis

533

Amino Acids

59.5

Weight (kDa)

7.31

Isoelectric Point (pI)

37.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	56 - 512	1.7e-83	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25180
malus_domestica	MD05G1169600.v1.1 MD05G1189900.v1.1 MD10G1157900.v1.1 MD10G1158100.v1.1 MD10G1176900.v1.1 MD10G1177100.v1.1
prunus_persica	Prupe.8G196800_v2.0.a1 Prupe.8G196900_v2.0.a1 Prupe.8G197000_v2.0.a1
pyrus_communis	pycom05g15710 pycom05g17500 pycom10g13610 pycom10g13620 pycom10g13630 pycom10g13660 pycom10g13670 pycom10g15440
rosa_chinensis	RchiOBHm_Chr6g0283821
rosa_laevigata	RLG00000012794
rosa_multiflora	Rmu_sc0001521.1_g000036
rosa_roxburghii	Rroxscaffold_7G00185160
rosa_rugosa	Rorug06G0158000
rosa_samantha	Rh6CG272900
rosa_wichuraiana	Rw6G023340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	245, 1253
AccB7I	CCANNNNNTGG	1 cut(s)	304
AccI	GTMKAC	1 cut(s)	534
AccII	CGCG	2 cut(s)	295, 453
AccIII	TCCGGA	1 cut(s)	459
AciI	CCGC	2 cut(s)	293, 1544
AclWI	GGATC	3 cut(s)	218, 1541, 1554
AcoI	YGGCCR	1 cut(s)	252
AcsI	RAATTY	1 cut(s)	260
AcuI	CTGAAG	1 cut(s)	1451
AcvI	CACGTG	1 cut(s)	517
AcyI	GRCGYC	1 cut(s)	246
AfaI	GTAC	4 cut(s)	382, 437, 1181, 1382
AfeI	AGCGCT	1 cut(s)	319
AfiI	CCNNNNNNNGG	4 cut(s)	194, 304, 466, 1264
AflII	CTTAAG	2 cut(s)	281, 1553
AgsI	TTSAA	5 cut(s)	503, 803, 1118, 1325, 1369
AjiI	CACGTC	1 cut(s)	1357
AjnI	CCWGG	2 cut(s)	175, 1414
AluBI	AGCT	7 cut(s)	50, 286, 816, 859, 879, 951, 1395
AluI	AGCT	7 cut(s)	50, 286, 816, 859, 879, 951, 1395
Alw21I	GWGCWC	1 cut(s)	642
Alw26I	GTCTC	1 cut(s)	137
AlwI	GGATC	3 cut(s)	218, 1541, 1554
Ama87I	CYCGRG	1 cut(s)	1396
Aor13HI	TCCGGA	1 cut(s)	459
Aor51HI	AGCGCT	1 cut(s)	319
AoxI	GGCC	6 cut(s)	185, 252, 824, 1305, 1499, 1557
ApeKI	GCWGC	3 cut(s)	89, 386, 876
ApoI	RAATTY	1 cut(s)	260
ArsI	GACNNNNNNTTYG	2 cut(s)	803, 835
AspLEI	GCGC	4 cut(s)	248, 297, 320, 453
AspS9I	GGNCC	8 cut(s)	266, 290, 440, 824, 1291, 1305, 1407, 1499
AsuC2I	CCSGG	2 cut(s)	676, 1289
AsuHPI	GGTGA	6 cut(s)	191, 574, 594, 1189, 1505, 1577
AsuII	TTCGAA	1 cut(s)	732
AvaI	CYCGRG	1 cut(s)	1396
AvaII	GGWCC	5 cut(s)	266, 290, 440, 1291, 1407
BaeGI	GKGCMC	3 cut(s)	1033, 1063, 1214
BaeI	ACNNNNGTAYC	2 cut(s)	1163, 1196
BamHI	GGATCC	1 cut(s)	1546
BanI	GGYRCC	2 cut(s)	245, 1253
BanII	GRGCYC	1 cut(s)	582
BauI	CACGAG	1 cut(s)	1205
BbrPI	CACGTG	1 cut(s)	517
Bbv12I	GWGCWC	1 cut(s)	642
BbvI	GCAGC	3 cut(s)	101, 398, 888
BccI	CCATC	3 cut(s)	686, 1104, 1490
BcgI	CGANNNNNNTGC	2 cut(s)	865, 899
BciT130I	CCWGG	2 cut(s)	177, 1416
BcnI	CCSGG	2 cut(s)	676, 1289
BcoDI	GTCTC	1 cut(s)	137
BfaI	CTAG	2 cut(s)	428, 1427
BfmI	CTRYAG	1 cut(s)	609
BfoI	RGCGCY	2 cut(s)	249, 321
BfrI	CTTAAG	2 cut(s)	281, 1553
BisI	GCNGC	3 cut(s)	90, 387, 877
BlpI	GCTNAGC	2 cut(s)	314, 872
BlsI	GCNGC	3 cut(s)	91, 388, 878
Bme1390I	CCNGG	4 cut(s)	177, 676, 1289, 1416
Bme18I	GGWCC	5 cut(s)	266, 290, 440, 1291, 1407
BmeT110I	CYCGRG	1 cut(s)	1396
BmgBI	CACGTC	1 cut(s)	1357
BmgT120I	GGNCC	8 cut(s)	266, 290, 440, 824, 1291, 1305, 1407, 1499
BmiI	GGNNCC	6 cut(s)	247, 442, 1255, 1292, 1293, 1548
BmrFI	CCNGG	4 cut(s)	177, 676, 1289, 1416
BmsI	GCATC	3 cut(s)	766, 775, 892
BoxI	GACNNNNGTC	1 cut(s)	161
BplI	GAGNNNNNCTC	4 cut(s)	470, 502, 848, 880
BpmI	CTGGAG	1 cut(s)	159
Bpu1102I	GCTNAGC	2 cut(s)	314, 872
Bpu14I	TTCGAA	1 cut(s)	732
BpuMI	CCSGG	2 cut(s)	676, 1289
BsaAI	YACGTR	1 cut(s)	517
BsaBI	GATNNNNATC	1 cut(s)	1224
BsaHI	GRCGYC	1 cut(s)	246
BsaI	GGTCTC	1 cut(s)	137
BsaJI	CCNNGG	3 cut(s)	249, 763, 1414
BsaWI	WCCGGW	1 cut(s)	459
Bsc4I	CCNNNNNNNGG	4 cut(s)	194, 304, 466, 1264
Bse118I	RCCGGY	2 cut(s)	389, 1501
Bse1I	ACTGG	2 cut(s)	227, 452
Bse8I	GATNNNNATC	1 cut(s)	1224
BseAI	TCCGGA	1 cut(s)	459
BseBI	CCWGG	2 cut(s)	177, 1416
BseDI	CCNNGG	3 cut(s)	249, 763, 1414
BseGI	GGATG	3 cut(s)	10, 456, 790
BseJI	GATNNNNATC	1 cut(s)	1224
BseLI	CCNNNNNNNGG	4 cut(s)	194, 304, 466, 1264
BseMII	CTCAG	3 cut(s)	488, 501, 1320
BseNI	ACTGG	2 cut(s)	227, 452
BseRI	GAGGAG	2 cut(s)	941, 1034
BseSI	GKGCMC	3 cut(s)	1033, 1063, 1214
BseXI	GCAGC	3 cut(s)	101, 398, 888
BseYI	CCCAGC	1 cut(s)	879
Bsh1236I	CGCG	2 cut(s)	295, 453
Bsh1285I	CGRYCG	1 cut(s)	377
BshFI	GGCC	6 cut(s)	187, 254, 826, 1307, 1501, 1559
BshNI	GGYRCC	2 cut(s)	245, 1253
BsiEI	CGRYCG	1 cut(s)	377
BsiHKAI	GWGCWC	1 cut(s)	642
BsiHKCI	CYCGRG	1 cut(s)	1396
BsiSI	CCGG	5 cut(s)	390, 460, 675, 1288, 1502
BsiWI	CGTACG	1 cut(s)	435
BslFI	GGGAC	4 cut(s)	149, 1175, 1229, 1304
BslI	CCNNNNNNNGG	4 cut(s)	194, 304, 466, 1264
BsmAI	GTCTC	1 cut(s)	137
BsmFI	GGGAC	4 cut(s)	149, 1175, 1229, 1304
BsnI	GGCC	6 cut(s)	187, 254, 826, 1307, 1501, 1559
Bso31I	GGTCTC	1 cut(s)	137
BsoBI	CYCGRG	1 cut(s)	1396
Bsp119I	TTCGAA	1 cut(s)	732
Bsp1286I	GDGCHC	5 cut(s)	582, 642, 1033, 1063, 1214
Bsp13I	TCCGGA	1 cut(s)	459
Bsp143I	GATC	4 cut(s)	149, 223, 270, 1546
Bsp1720I	GCTNAGC	2 cut(s)	314, 872
Bsp19I	CCATGG	1 cut(s)	249
BspACI	CCGC	2 cut(s)	293, 1544
BspANI	GGCC	6 cut(s)	187, 254, 826, 1307, 1501, 1559
BspCNI	CTCAG	3 cut(s)	487, 500, 1321
BspEI	TCCGGA	1 cut(s)	459
BspFNI	CGCG	2 cut(s)	295, 453
BspHI	TCATGA	2 cut(s)	837, 1338
BspLI	GGNNCC	6 cut(s)	247, 442, 1255, 1292, 1293, 1548
BspPI	GGATC	3 cut(s)	218, 1541, 1554
BspT104I	TTCGAA	1 cut(s)	732
BspT107I	GGYRCC	2 cut(s)	245, 1253
BspTI	CTTAAG	2 cut(s)	281, 1553
BspTNI	GGTCTC	1 cut(s)	137
BsrFI	RCCGGY	2 cut(s)	389, 1501
BsrI	ACTGG	2 cut(s)	227, 452
BssAI	RCCGGY	2 cut(s)	389, 1501
BssECI	CCNNGG	3 cut(s)	249, 763, 1414
BssMI	GATC	4 cut(s)	149, 223, 270, 1546
BssNI	GRCGYC	1 cut(s)	246
BssSI	CACGAG	1 cut(s)	1205
BssT1I	CCWWGG	2 cut(s)	249, 763
Bst2BI	CACGAG	1 cut(s)	1205
Bst2UI	CCWGG	2 cut(s)	177, 1416
Bst4CI	ACNGT	2 cut(s)	610, 1201
Bst6I	CTCTTC	2 cut(s)	674, 1587
BstACI	GRCGYC	1 cut(s)	246
BstAFI	CTTAAG	2 cut(s)	281, 1553
BstBAI	YACGTR	1 cut(s)	517
BstBI	TTCGAA	1 cut(s)	732
BstC8I	GCNNGC	2 cut(s)	391, 522
BstDEI	CTNAG	6 cut(s)	99, 314, 474, 487, 872, 1329
BstDSI	CCRYGG	1 cut(s)	249
BstEII	GGTNACC	1 cut(s)	197
BstENI	CCTNNNNNAGG	1 cut(s)	192
BstF5I	GGATG	3 cut(s)	10, 456, 790
BstFNI	CGCG	2 cut(s)	295, 453
BstH2I	RGCGCY	2 cut(s)	249, 321
BstHHI	GCGC	4 cut(s)	248, 297, 320, 453
BstKTI	GATC	4 cut(s)	152, 226, 273, 1549
BstMAI	GTCTC	1 cut(s)	137
BstMBI	GATC	4 cut(s)	149, 223, 270, 1546
BstMCI	CGRYCG	1 cut(s)	377
BstMWI	GCNNNNNNNGC	4 cut(s)	292, 422, 1005, 1565
BstNI	CCWGG	2 cut(s)	177, 1416
BstNSI	RCATGY	1 cut(s)	1439
BstPAI	GACNNNNGTC	1 cut(s)	161
BstPI	GGTNACC	1 cut(s)	197
BstSCI	CCNGG	4 cut(s)	175, 674, 1287, 1414
BstSFI	CTRYAG	1 cut(s)	609
BstSLI	GKGCMC	3 cut(s)	1033, 1063, 1214
BstUI	CGCG	2 cut(s)	295, 453
BstV1I	GCAGC	3 cut(s)	101, 398, 888
BstX2I	RGATCY	2 cut(s)	223, 1546
BstXI	CCANNNNNNTGG	2 cut(s)	183, 1302
BstYI	RGATCY	2 cut(s)	223, 1546
BsuRI	GGCC	6 cut(s)	187, 254, 826, 1307, 1501, 1559
BtgI	CCRYGG	1 cut(s)	249
BtgZI	GCGATG	2 cut(s)	573, 920
BtrI	CACGTC	1 cut(s)	1357
BtsCI	GGATG	3 cut(s)	10, 456, 790
BtsI	GCAGTG	1 cut(s)	721
BtsIMutI	CAGTG	1 cut(s)	721
Cac8I	GCNNGC	2 cut(s)	391, 522
CciI	TCATGA	2 cut(s)	837, 1338
CfoI	GCGC	4 cut(s)	248, 297, 320, 453
Cfr10I	RCCGGY	2 cut(s)	389, 1501
Cfr13I	GGNCC	8 cut(s)	266, 290, 440, 824, 1291, 1305, 1407, 1499
CpoI	CGGWCCG	1 cut(s)	266
Csp6I	GTAC	4 cut(s)	381, 436, 1180, 1381
CspI	CGGWCCG	1 cut(s)	266
CviAII	CATG	9 cut(s)	183, 215, 250, 838, 1026, 1241, 1339, 1349, 1436
CviQI	GTAC	4 cut(s)	381, 436, 1180, 1381
DdeI	CTNAG	6 cut(s)	99, 314, 474, 487, 872, 1329
DinI	GGCGCC	1 cut(s)	247
DpnI	GATC	4 cut(s)	151, 225, 272, 1548
DpnII	GATC	4 cut(s)	149, 223, 270, 1546
EaeI	YGGCCR	1 cut(s)	252
Eam1104I	CTCTTC	2 cut(s)	674, 1587
EarI	CTCTTC	2 cut(s)	674, 1587
Eco130I	CCWWGG	2 cut(s)	249, 763
Eco147I	AGGCCT	1 cut(s)	1559
Eco24I	GRGCYC	1 cut(s)	582
Eco31I	GGTCTC	1 cut(s)	137
Eco32I	GATATC	1 cut(s)	912
Eco47I	GGWCC	5 cut(s)	266, 290, 440, 1291, 1407
Eco47III	AGCGCT	1 cut(s)	319
Eco57I	CTGAAG	1 cut(s)	1451
Eco72I	CACGTG	1 cut(s)	517
Eco88I	CYCGRG	1 cut(s)	1396
Eco91I	GGTNACC	1 cut(s)	197
EcoNI	CCTNNNNNAGG	1 cut(s)	192
EcoO109I	RGGNCCY	2 cut(s)	1291, 1407
EcoO65I	GGTNACC	1 cut(s)	197
EcoRII	CCWGG	2 cut(s)	175, 1414
EcoRV	GATATC	1 cut(s)	912
EcoT14I	CCWWGG	2 cut(s)	249, 763
EcoT22I	ATGCAT	2 cut(s)	510, 759
EcoT38I	GRGCYC	1 cut(s)	582
EgeI	GGCGCC	1 cut(s)	247
EheI	GGCGCC	1 cut(s)	247
ErhI	CCWWGG	2 cut(s)	249, 763
FaeI	CATG	9 cut(s)	186, 218, 253, 841, 1029, 1244, 1342, 1352, 1439
FaqI	GGGAC	4 cut(s)	149, 1175, 1229, 1304
FatI	CATG	9 cut(s)	182, 214, 249, 837, 1025, 1240, 1338, 1348, 1435
FblI	GTMKAC	1 cut(s)	534
Fnu4HI	GCNGC	3 cut(s)	90, 387, 877
FokI	GGATG	3 cut(s)	17, 443, 797
FriOI	GRGCYC	1 cut(s)	582
Fsp4HI	GCNGC	3 cut(s)	90, 387, 877
FspBI	CTAG	2 cut(s)	428, 1427
GlaI	GCGC	4 cut(s)	247, 296, 319, 452
GluI	GCNGC	3 cut(s)	90, 387, 877
GsaI	CCCAGC	1 cut(s)	883
GsuI	CTGGAG	1 cut(s)	159
HaeII	RGCGCY	2 cut(s)	249, 321
HaeIII	GGCC	6 cut(s)	187, 254, 826, 1307, 1501, 1559
HapII	CCGG	5 cut(s)	390, 460, 675, 1288, 1502
HhaI	GCGC	4 cut(s)	248, 297, 320, 453
Hin1I	GRCGYC	1 cut(s)	246
Hin1II	CATG	9 cut(s)	186, 218, 253, 841, 1029, 1244, 1342, 1352, 1439
Hin6I	GCGC	4 cut(s)	246, 295, 318, 451
HinP1I	GCGC	4 cut(s)	246, 295, 318, 451
HincII	GTYRAC	3 cut(s)	156, 535, 1270
HindII	GTYRAC	3 cut(s)	156, 535, 1270
HindIII	AAGCTT	1 cut(s)	284
HinfI	GANTC	5 cut(s)	536, 863, 1121, 1220, 1488
HpaII	CCGG	5 cut(s)	390, 460, 675, 1288, 1502
HphI	GGTGA	6 cut(s)	191, 574, 594, 1189, 1505, 1577
Hpy166II	GTNNAC	5 cut(s)	156, 535, 1204, 1270, 1360
Hpy188I	TCNGA	5 cut(s)	270, 691, 784, 1126, 1134
Hpy188III	TCNNGA	5 cut(s)	460, 476, 838, 1118, 1339
Hpy8I	GTNNAC	5 cut(s)	156, 535, 1204, 1270, 1360
HpyAV	CCTTC	4 cut(s)	212, 1139, 1398, 1586
HpyCH4III	ACNGT	2 cut(s)	610, 1201
HpyCH4IV	ACGT	4 cut(s)	516, 647, 1095, 1356
HpyCH4V	TGCA	8 cut(s)	74, 508, 619, 745, 757, 1019, 1285, 1435
HpyF10VI	GCNNNNNNNGC	4 cut(s)	292, 422, 1005, 1565
HpyF3I	CTNAG	6 cut(s)	99, 314, 474, 487, 872, 1329
HpySE526I	ACGT	4 cut(s)	516, 647, 1095, 1356
Hsp92I	GRCGYC	1 cut(s)	246
Hsp92II	CATG	9 cut(s)	186, 218, 253, 841, 1029, 1244, 1342, 1352, 1439
HspAI	GCGC	4 cut(s)	246, 295, 318, 451
KasI	GGCGCC	1 cut(s)	245
KflI	GGGWCCC	1 cut(s)	1291
Kpn2I	TCCGGA	1 cut(s)	459
KroI	GCCGGC	1 cut(s)	389
KroNI	GCCGGC	1 cut(s)	391
Kzo9I	GATC	4 cut(s)	149, 223, 270, 1546
LmnI	GCTCC	3 cut(s)	179, 856, 1573
Lsp1109I	GCAGC	3 cut(s)	101, 398, 888
LweI	GCATC	3 cut(s)	766, 775, 892
MaeI	CTAG	2 cut(s)	428, 1427
MaeII	ACGT	4 cut(s)	516, 647, 1095, 1356
MaeIII	GTNAC	5 cut(s)	197, 404, 512, 890, 1103
MalI	GATC	4 cut(s)	151, 225, 272, 1548
MboI	GATC	4 cut(s)	149, 223, 270, 1546
MboII	GAAGA	5 cut(s)	124, 127, 539, 691, 1574
MflI	RGATCY	2 cut(s)	223, 1546
MhlI	GDGCHC	5 cut(s)	582, 642, 1033, 1063, 1214
MluCI	AATT	7 cut(s)	30, 237, 260, 551, 769, 1082, 1280
Mly113I	GGCGCC	1 cut(s)	246
MlyI	GAGTC	3 cut(s)	530, 857, 1497
MmeI	TCCRAC	3 cut(s)	762, 939, 1149
Mph1103I	ATGCAT	2 cut(s)	510, 759
MroI	TCCGGA	1 cut(s)	459
MroNI	GCCGGC	1 cut(s)	389
MseI	TTAA	5 cut(s)	36, 282, 651, 852, 1554
MslI	CAYNNNNRTG	3 cut(s)	659, 951, 1024
MspA1I	CMGCKG	1 cut(s)	879
MspCI	CTTAAG	2 cut(s)	281, 1553
MspI	CCGG	5 cut(s)	390, 460, 675, 1288, 1502
MspR9I	CCNGG	4 cut(s)	177, 676, 1289, 1416
MvaI	CCWGG	2 cut(s)	177, 1416
MvnI	CGCG	2 cut(s)	295, 453
MwoI	GCNNNNNNNGC	4 cut(s)	292, 422, 1005, 1565
NaeI	GCCGGC	1 cut(s)	391
NarI	GGCGCC	1 cut(s)	246
NciI	CCSGG	2 cut(s)	676, 1289
NcoI	CCATGG	1 cut(s)	249
NdeII	GATC	4 cut(s)	149, 223, 270, 1546
NgoMIV	GCCGGC	1 cut(s)	389
NlaIII	CATG	9 cut(s)	186, 218, 253, 841, 1029, 1244, 1342, 1352, 1439
NlaIV	GGNNCC	6 cut(s)	247, 442, 1255, 1292, 1293, 1548
NmuCI	GTSAC	4 cut(s)	197, 512, 890, 1103
NsiI	ATGCAT	2 cut(s)	510, 759
NspI	RCATGY	1 cut(s)	1439
NspV	TTCGAA	1 cut(s)	732
PagI	TCATGA	2 cut(s)	837, 1338
PceI	AGGCCT	1 cut(s)	1559
PcsI	WCGNNNNNNNCGW	1 cut(s)	522
PdiI	GCCGGC	1 cut(s)	391
PfeI	GAWTC	2 cut(s)	1121, 1220
Pfl23II	CGTACG	1 cut(s)	435
PflMI	CCANNNNNTGG	1 cut(s)	304
PfoI	TCCNGGA	1 cut(s)	175
PkrI	GCNGC	3 cut(s)	91, 388, 878
PleI	GAGTC	3 cut(s)	530, 857, 1496
PluTI	GGCGCC	1 cut(s)	249
PmaCI	CACGTG	1 cut(s)	517
PmlI	CACGTG	1 cut(s)	517
PpsI	GAGTC	3 cut(s)	530, 857, 1496
Ppu21I	YACGTR	1 cut(s)	517
PpuMI	RGGWCCY	2 cut(s)	1291, 1407
PshAI	GACNNNNGTC	1 cut(s)	161
Psp5II	RGGWCCY	2 cut(s)	1291, 1407
Psp6I	CCWGG	2 cut(s)	175, 1414
PspCI	CACGTG	1 cut(s)	517
PspEI	GGTNACC	1 cut(s)	197
PspFI	CCCAGC	1 cut(s)	879
PspGI	CCWGG	2 cut(s)	175, 1414
PspLI	CGTACG	1 cut(s)	435
PspN4I	GGNNCC	6 cut(s)	247, 442, 1255, 1292, 1293, 1548
PspPI	GGNCC	8 cut(s)	266, 290, 440, 824, 1291, 1305, 1407, 1499
PspPPI	RGGWCCY	2 cut(s)	1291, 1407
PsrI	GAACNNNNNNTAC	2 cut(s)	1127, 1159
PsuI	RGATCY	2 cut(s)	223, 1546
PvuII	CAGCTG	1 cut(s)	879
RsaI	GTAC	4 cut(s)	382, 437, 1181, 1382
RsaNI	GTAC	4 cut(s)	381, 436, 1180, 1381
RseI	CAYNNNNRTG	3 cut(s)	659, 951, 1024
Rsr2I	CGGWCCG	1 cut(s)	266
RsrII	CGGWCCG	1 cut(s)	266
SalI	GTCGAC	1 cut(s)	533
SaqAI	TTAA	5 cut(s)	36, 282, 651, 852, 1554
SatI	GCNGC	3 cut(s)	90, 387, 877
Sau3AI	GATC	4 cut(s)	149, 223, 270, 1546
Sau96I	GGNCC	8 cut(s)	266, 290, 440, 824, 1291, 1305, 1407, 1499
SchI	GAGTC	3 cut(s)	530, 857, 1497
ScrFI	CCNGG	4 cut(s)	177, 676, 1289, 1416
SduI	GDGCHC	5 cut(s)	582, 642, 1033, 1063, 1214
SfaNI	GCATC	3 cut(s)	766, 775, 892
SfcI	CTRYAG	1 cut(s)	609
SfoI	GGCGCC	1 cut(s)	247
SfuI	TTCGAA	1 cut(s)	732
SinI	GGWCC	5 cut(s)	266, 290, 440, 1291, 1407
SmiMI	CAYNNNNRTG	3 cut(s)	659, 951, 1024
SmlI	CTYRAG	2 cut(s)	281, 1553
SmoI	CTYRAG	2 cut(s)	281, 1553
Sse9I	AATT	7 cut(s)	30, 237, 260, 551, 769, 1082, 1280
SseBI	AGGCCT	1 cut(s)	1559
SsiI	CCGC	2 cut(s)	293, 1544
SspDI	GGCGCC	1 cut(s)	245
SspMI	CTAG	2 cut(s)	428, 1427
StuI	AGGCCT	1 cut(s)	1559
StyD4I	CCNGG	4 cut(s)	175, 674, 1287, 1414
StyI	CCWWGG	2 cut(s)	249, 763
TaaI	ACNGT	2 cut(s)	610, 1201
TaiI	ACGT	4 cut(s)	519, 650, 1098, 1359
TaqI	TCGA	4 cut(s)	534, 558, 732, 885
TaqII	GACCGA	3 cut(s)	254, 391, 1358
TasI	AATT	7 cut(s)	30, 237, 260, 551, 769, 1082, 1280
TatI	WGTACW	2 cut(s)	380, 1380
TfiI	GAWTC	2 cut(s)	1121, 1220
Tru1I	TTAA	5 cut(s)	36, 282, 651, 852, 1554
Tru9I	TTAA	5 cut(s)	36, 282, 651, 852, 1554
TscAI	CASTG	1 cut(s)	721
TseFI	GTSAC	4 cut(s)	197, 512, 890, 1103
TseI	GCWGC	3 cut(s)	89, 386, 876
Tsp45I	GTSAC	4 cut(s)	197, 512, 890, 1103
TspDTI	ATGAA	6 cut(s)	181, 194, 532, 826, 854, 1327
TspGWI	ACGGA	3 cut(s)	453, 1229, 1377
TspRI	CASTG	1 cut(s)	721
Van91I	CCANNNNNTGG	1 cut(s)	304
Vha464I	CTTAAG	2 cut(s)	281, 1553
VpaK11BI	GGWCC	5 cut(s)	266, 290, 440, 1291, 1407
XagI	CCTNNNNNAGG	1 cut(s)	192
XapI	RAATTY	1 cut(s)	260
XceI	RCATGY	1 cut(s)	1439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	801
XmiI	GTMKAC	1 cut(s)	534
XspI	CTAG	2 cut(s)	428, 1427
Zsp2I	ATGCAT	2 cut(s)	510, 759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.