pycom10g13610

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006975
Physical Location & Seq
Reverse (-)
17097721 .. 17099456
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g13610.1

Sequence Viewer

Length: 1278 bp
ATGGAGTCAACAATTTCTCCATTCCAAATCATTCACACAGTTTTAGCTTCTTTAATTCTCTATGGTGCCTTGAGAATTATAGCAATGGCAAACACTAAGAAGAACACAACCAACAAAAAGATCATGATGCCTAAAGTCCCTCAACCCTCAGGAGCATGGCCCTTAATAGGTCACCTTCCTCTGCTACGTGCAGAAAACCCAATTGCACTTACACTCGGAGCCATGGCTGATCAACACGGACCCGTCTACTCCCTGAAGCTTGGACAGCACGAATTGCTTGTTCTCAGCGCCTGGGAACAAGTCCAAGAATTGTTCACAAAAAAGGACAGAGTTTTCGCCACCCGGCCGACTACAGCCGGCGGAAAGTACTTGGGCTATGACAATGCGACCTTCGCCCTTTCCCCGTACGGAGACTACTGGCGCGAGGTCCGTAAATTGGCCACCATGGAGCTTCTCTCGAGCAAAAGAGTTAAAACCCTAAGCCACGTCAGAGGCTCAGAAGTTGACTCCTTCATCAAAACTTTGCACTCACTAGTTTGCACAAAAGATAATGTGGATCCAAGTTCCACATCATCGTCGCCAGTGCATATGAGTGAGTTATTAGAGCACTTGACGTTTAACATAAACGTGAAGTTAATTGCTGGGAAGAGATTTACAGCTGGCCAGTATAACGAGAAAAACGGTGACGTTTGGCGTTTTGAAAAGGCAGTAAAAGAGACTTTGCATCTCTTTGGTGTTTTTGTCTGGTCGGATGCTATGCCATGTTTTGAGTGGCTGGACGGTTGGTTTGGTCACGTGAGTTCCATGAAGAAATGTTTTAAGGAACTGGACTACGTACTTGGGAAATGGCTCGAAGAACATCGACAAAGAAGATTGGAAAGCAAGAACAATGGGGTTGAGAGTGACTTGATGGATGTTATGTTATCCAACTTTGAGGAGGATGAAATTTATGGCCATAACCGTGATACTGTCATCAAAGCAACAGCACTGGTGCTAATGGCAACTGGCACAGAAAGCACATCTGTGACACTAACATGGGCAATCTCCTTACTCCTCAACAACCAAAAAGTCCTCAAATCTGCCCAGGAAGAGTTGGACATCCACGTAGGAAAAGACCGATGGGTTCAAGAATCGATCTCACAAACCTCAAATACCTCCAAGCCATTCTCAAGGAAACCCTACGCTTATACCCACCTGGCCCAATCACAGGCCTCCGTGAGGCCATGGAGGACTGTCATCTCGGTGGTTACTTCGTCCCTAAAGGCACTCGTTTACTAG

Protein Analysis

426

Amino Acids

47.94

Weight (kDa)

8.96

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 376 2.3e-37 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 423
AciI CCGC 1 cut(s) 360
AclWI GGATC 2 cut(s) 551, 564
AcoI YGGCCR 4 cut(s) 344, 438, 661, 952
AcsI RAATTY 1 cut(s) 945
AcuI CTGAAG 1 cut(s) 275
AcvI CACGTG 1 cut(s) 796
AfaI GTAC 3 cut(s) 368, 407, 837
AfiI CCNNNNNNNGG 4 cut(s) 167, 436, 1207, 1218
AgsI TTSAA 2 cut(s) 701, 1127
AhlI ACTAGT 1 cut(s) 532
AjiI CACGTC 1 cut(s) 487
AjnI CCWGG 3 cut(s) 290, 1083, 1194
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 4 cut(s) 47, 259, 451, 659
AluI AGCT 4 cut(s) 47, 259, 451, 659
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 2 cut(s) 405, 710
AlwI GGATC 2 cut(s) 551, 564
AlwNI CAGNNNCTG 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 457
AoxI GGCC 8 cut(s) 158, 344, 438, 661, 952, 1197, 1209, 1220
ApoI RAATTY 1 cut(s) 945
ArsI GACNNNNNNTTYG 4 cut(s) 317, 349, 770, 802
AspLEI GCGC 2 cut(s) 290, 423
AspS9I GGNCC 4 cut(s) 159, 239, 427, 1198
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 164, 695
AvaI CYCGRG 1 cut(s) 457
AvaII GGWCC 2 cut(s) 239, 427
AxyI CCTNAGG 1 cut(s) 148
BalI TGGCCA 3 cut(s) 440, 663, 954
BamHI GGATCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 65
BbrPI CACGTG 1 cut(s) 796
Bbv12I GWGCWC 1 cut(s) 609
BccI CCATC 2 cut(s) 904, 1113
BciT130I CCWGG 3 cut(s) 292, 1085, 1196
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 405, 710
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 2 cut(s) 533, 1276
BfmI CTRYAG 1 cut(s) 351
BfoI RGCGCY 1 cut(s) 291
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 4 cut(s) 292, 343, 1085, 1196
Bme18I GGWCC 2 cut(s) 239, 427
BmeT110I CYCGRG 1 cut(s) 457
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 4 cut(s) 159, 239, 427, 1198
BmiI GGNNCC 4 cut(s) 67, 220, 241, 558
BmrFI CCNGG 4 cut(s) 292, 343, 1085, 1196
BmsI GCATC 3 cut(s) 117, 733, 742
BplI GAGNNNNNCTC 2 cut(s) 440, 472
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 2 cut(s) 91, 1153
BpuMI CCSGG 1 cut(s) 343
Bsa29I ATCGAT 1 cut(s) 1133
BsaAI YACGTR 4 cut(s) 188, 796, 835, 1105
BsaJI CCNNGG 5 cut(s) 222, 291, 444, 1083, 1223
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 436, 1207, 1218
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 6 cut(s) 422, 581, 664, 831, 993, 1009
Bse21I CCTNAGG 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 90
BseBI CCWGG 3 cut(s) 292, 1085, 1196
BseCI ATCGAT 1 cut(s) 1133
BseDI CCNNGG 5 cut(s) 222, 291, 444, 1083, 1223
BseGI GGATG 4 cut(s) 757, 919, 946, 1098
BseLI CCNNNNNNNGG 4 cut(s) 167, 436, 1207, 1218
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 3 cut(s) 162, 298, 510
BseNI ACTGG 6 cut(s) 422, 581, 664, 831, 993, 1009
BseRI GAGGAG 2 cut(s) 950, 1043
BseX3I CGGCCG 1 cut(s) 344
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 423
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 8 cut(s) 160, 346, 440, 663, 954, 1199, 1211, 1222
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 1133
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 2 cut(s) 343, 357
BsiWI CGTACG 1 cut(s) 405
BslFI GGGAC 2 cut(s) 122, 1240
BslI CCNNNNNNNGG 4 cut(s) 167, 436, 1207, 1218
BsmAI GTCTC 2 cut(s) 405, 710
BsmFI GGGAC 2 cut(s) 122, 1240
BsnI GGCC 8 cut(s) 160, 346, 440, 663, 954, 1199, 1211, 1222
BsoBI CYCGRG 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 4 cut(s) 120, 229, 556, 1134
Bsp19I CCATGG 3 cut(s) 222, 444, 1223
BspACI CCGC 1 cut(s) 360
BspANI GGCC 8 cut(s) 160, 346, 440, 663, 954, 1199, 1211, 1222
BspCNI CTCAG 3 cut(s) 161, 297, 509
BspDI ATCGAT 1 cut(s) 1133
BspFNI CGCG 1 cut(s) 423
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 4 cut(s) 67, 220, 241, 558
BspPI GGATC 2 cut(s) 551, 564
BspT107I GGYRCC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 6 cut(s) 422, 581, 664, 831, 993, 1009
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 5 cut(s) 222, 291, 444, 1083, 1223
BssMI GATC 4 cut(s) 120, 229, 556, 1134
BssT1I CCWWGG 3 cut(s) 222, 444, 1223
Bst2UI CCWGG 3 cut(s) 292, 1085, 1196
Bst4CI ACNGT 6 cut(s) 40, 683, 782, 962, 970, 1234
Bst6I CTCTTC 2 cut(s) 641, 1083
BstAPI GCANNNNNTGC 1 cut(s) 274
BstBAI YACGTR 4 cut(s) 188, 796, 835, 1105
BstC8I GCNNGC 2 cut(s) 358, 661
BstDEI CTNAG 5 cut(s) 96, 148, 284, 479, 496
BstDSI CCRYGG 3 cut(s) 222, 444, 1223
BstEII GGTNACC 1 cut(s) 170
BstENI CCTNNNNNAGG 1 cut(s) 1216
BstF5I GGATG 4 cut(s) 757, 919, 946, 1098
BstFNI CGCG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 290, 423
BstKTI GATC 4 cut(s) 123, 232, 559, 1137
BstMAI GTCTC 2 cut(s) 405, 710
BstMBI GATC 4 cut(s) 120, 229, 556, 1134
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 4 cut(s) 265, 274, 392, 1014
BstNI CCWGG 3 cut(s) 292, 1085, 1196
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 4 cut(s) 290, 341, 1083, 1194
BstSFI CTRYAG 1 cut(s) 351
BstSNI TACGTA 1 cut(s) 835
BstUI CGCG 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BstZI CGGCCG 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 1133
Bsu36I CCTNAGG 1 cut(s) 148
BsuRI GGCC 8 cut(s) 160, 346, 440, 663, 954, 1199, 1211, 1222
BsuTUI ATCGAT 1 cut(s) 1133
BtgI CCRYGG 3 cut(s) 222, 444, 1223
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 4 cut(s) 757, 919, 946, 1098
BtsIMutI CAGTG 2 cut(s) 588, 986
Cac8I GCNNGC 2 cut(s) 358, 661
CaiI CAGNNNCTG 1 cut(s) 291
CciI TCATGA 1 cut(s) 123
CfoI GCGC 2 cut(s) 290, 423
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 4 cut(s) 159, 239, 427, 1198
ClaI ATCGAT 1 cut(s) 1133
Csp6I GTAC 3 cut(s) 367, 406, 836
CviAII CATG 8 cut(s) 124, 156, 223, 445, 762, 805, 1035, 1224
CviQI GTAC 3 cut(s) 367, 406, 836
DdeI CTNAG 5 cut(s) 96, 148, 284, 479, 496
DpnI GATC 4 cut(s) 122, 231, 558, 1136
DpnII GATC 4 cut(s) 120, 229, 556, 1134
EaeI YGGCCR 4 cut(s) 344, 438, 661, 952
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 641, 1083
EarI CTCTTC 2 cut(s) 641, 1083
EciI GGCGGA 1 cut(s) 375
EclXI CGGCCG 1 cut(s) 344
Eco105I TACGTA 1 cut(s) 835
Eco130I CCWWGG 3 cut(s) 222, 444, 1223
Eco147I AGGCCT 1 cut(s) 1211
Eco47I GGWCC 2 cut(s) 239, 427
Eco52I CGGCCG 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 275
Eco72I CACGTG 1 cut(s) 796
Eco81I CCTNAGG 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 457
Eco91I GGTNACC 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 170
EcoRII CCWGG 3 cut(s) 290, 1083, 1194
EcoT14I CCWWGG 3 cut(s) 222, 444, 1223
ErhI CCWWGG 3 cut(s) 222, 444, 1223
FaeI CATG 8 cut(s) 127, 159, 226, 448, 765, 808, 1038, 1227
FaqI GGGAC 2 cut(s) 122, 1240
FatI CATG 8 cut(s) 123, 155, 222, 444, 761, 804, 1034, 1223
FauNDI CATATG 1 cut(s) 588
FbaI TGATCA 1 cut(s) 229
FblI GTMKAC 1 cut(s) 246
FokI GGATG 4 cut(s) 764, 926, 953, 1085
FspBI CTAG 2 cut(s) 533, 1276
GlaI GCGC 2 cut(s) 289, 422
GsaI CCCAGC 1 cut(s) 645
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 8 cut(s) 160, 346, 440, 663, 954, 1199, 1211, 1222
HapII CCGG 2 cut(s) 343, 357
HhaI GCGC 2 cut(s) 290, 423
Hin1II CATG 8 cut(s) 127, 159, 226, 448, 765, 808, 1038, 1227
Hin6I GCGC 2 cut(s) 288, 421
HinP1I GCGC 2 cut(s) 288, 421
HincII GTYRAC 2 cut(s) 9, 505
HindII GTYRAC 2 cut(s) 9, 505
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 3 cut(s) 5, 506, 1130
HpaII CCGG 2 cut(s) 343, 357
HphI GGTGA 2 cut(s) 164, 695
Hpy166II GTNNAC 5 cut(s) 9, 247, 315, 505, 1273
Hpy188I TCNGA 4 cut(s) 218, 491, 499, 751
Hpy188III TCNNGA 4 cut(s) 124, 150, 457, 1127
Hpy8I GTNNAC 5 cut(s) 9, 247, 315, 505, 1273
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 3 cut(s) 185, 400, 520
HpyCH4III ACNGT 6 cut(s) 40, 683, 782, 962, 970, 1234
HpyCH4IV ACGT 8 cut(s) 187, 486, 614, 627, 687, 795, 834, 1104
HpyCH4V TGCA 6 cut(s) 191, 206, 526, 540, 586, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 265, 274, 392, 1014
HpyF3I CTNAG 5 cut(s) 96, 148, 284, 479, 496
HpySE526I ACGT 8 cut(s) 187, 486, 614, 627, 687, 795, 834, 1104
Hsp92II CATG 8 cut(s) 127, 159, 226, 448, 765, 808, 1038, 1227
HspAI GCGC 2 cut(s) 288, 421
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 4 cut(s) 120, 229, 556, 1134
LmnI GCTCC 3 cut(s) 152, 218, 448
LweI GCATC 3 cut(s) 117, 733, 742
MaeI CTAG 2 cut(s) 533, 1276
MaeII ACGT 8 cut(s) 187, 486, 614, 627, 687, 795, 834, 1104
MaeIII GTNAC 6 cut(s) 170, 683, 791, 902, 1024, 1246
MalI GATC 4 cut(s) 122, 231, 558, 1136
MboI GATC 4 cut(s) 120, 229, 556, 1134
MboII GAAGA 6 cut(s) 112, 658, 820, 866, 882, 1100
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 3 cut(s) 440, 663, 954
MluCI AATT 9 cut(s) 12, 54, 75, 201, 272, 308, 434, 636, 945
MluNI TGGCCA 3 cut(s) 440, 663, 954
MlyI GAGTC 2 cut(s) 14, 500
MmeI TCCRAC 3 cut(s) 729, 951, 1074
Mox20I TGGCCA 3 cut(s) 440, 663, 954
MroNI GCCGGC 1 cut(s) 356
MscI TGGCCA 3 cut(s) 440, 663, 954
MseI TTAA 6 cut(s) 53, 164, 471, 618, 635, 819
MslI CAYNNNNRTG 6 cut(s) 591, 626, 960, 1022, 1033, 1241
Msp20I TGGCCA 3 cut(s) 440, 663, 954
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 2 cut(s) 343, 357
MspR9I CCNGG 4 cut(s) 292, 343, 1085, 1196
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 3 cut(s) 292, 1085, 1196
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 4 cut(s) 265, 274, 392, 1014
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 1 cut(s) 343
NcoI CCATGG 3 cut(s) 222, 444, 1223
NdeI CATATG 1 cut(s) 588
NdeII GATC 4 cut(s) 120, 229, 556, 1134
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 8 cut(s) 127, 159, 226, 448, 765, 808, 1038, 1227
NlaIV GGNNCC 4 cut(s) 67, 220, 241, 558
NmuCI GTSAC 5 cut(s) 170, 683, 791, 902, 1024
OliI CACNNNNGTG 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 457
PagI TCATGA 1 cut(s) 123
PceI AGGCCT 1 cut(s) 1211
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 1130
Pfl23II CGTACG 1 cut(s) 405
PleI GAGTC 2 cut(s) 13, 500
PmaCI CACGTG 1 cut(s) 796
PmlI CACGTG 1 cut(s) 796
PpsI GAGTC 2 cut(s) 13, 500
Ppu21I YACGTR 4 cut(s) 188, 796, 835, 1105
Psp6I CCWGG 3 cut(s) 290, 1083, 1194
PspCI CACGTG 1 cut(s) 796
PspEI GGTNACC 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 3 cut(s) 290, 1083, 1194
PspLI CGTACG 1 cut(s) 405
PspN4I GGNNCC 4 cut(s) 67, 220, 241, 558
PspPI GGNCC 4 cut(s) 159, 239, 427, 1198
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 556
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 3 cut(s) 368, 407, 837
RsaNI GTAC 3 cut(s) 367, 406, 836
RseI CAYNNNNRTG 6 cut(s) 591, 626, 960, 1022, 1033, 1241
SaqAI TTAA 6 cut(s) 53, 164, 471, 618, 635, 819
Sau3AI GATC 4 cut(s) 120, 229, 556, 1134
Sau96I GGNCC 4 cut(s) 159, 239, 427, 1198
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 2 cut(s) 14, 500
ScrFI CCNGG 4 cut(s) 292, 343, 1085, 1196
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 3 cut(s) 117, 733, 742
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 457
SinI GGWCC 2 cut(s) 239, 427
SlaI CTCGAG 1 cut(s) 457
SmiMI CAYNNNNRTG 6 cut(s) 591, 626, 960, 1022, 1033, 1241
SmlI CTYRAG 3 cut(s) 70, 457, 1168
SmoI CTYRAG 3 cut(s) 70, 457, 1168
SnaBI TACGTA 1 cut(s) 835
SpeI ACTAGT 1 cut(s) 532
Sse9I AATT 9 cut(s) 12, 54, 75, 201, 272, 308, 434, 636, 945
SseBI AGGCCT 1 cut(s) 1211
SsiI CCGC 1 cut(s) 360
SspMI CTAG 2 cut(s) 533, 1276
StuI AGGCCT 1 cut(s) 1211
StyD4I CCNGG 4 cut(s) 290, 341, 1083, 1194
StyI CCWWGG 3 cut(s) 222, 444, 1223
TaaI ACNGT 6 cut(s) 40, 683, 782, 962, 970, 1234
TaiI ACGT 8 cut(s) 190, 489, 617, 630, 690, 798, 837, 1107
TaqI TCGA 4 cut(s) 458, 852, 862, 1133
TaqII GACCGA 1 cut(s) 1131
TasI AATT 9 cut(s) 12, 54, 75, 201, 272, 308, 434, 636, 945
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 1 cut(s) 1130
Tru1I TTAA 6 cut(s) 53, 164, 471, 618, 635, 819
Tru9I TTAA 6 cut(s) 53, 164, 471, 618, 635, 819
TscAI CASTG 2 cut(s) 588, 993
TseFI GTSAC 5 cut(s) 170, 683, 791, 902, 1024
Tsp45I GTSAC 5 cut(s) 170, 683, 791, 902, 1024
TspDTI ATGAA 3 cut(s) 502, 821, 957
TspGWI ACGGA 4 cut(s) 252, 419, 423, 1204
TspRI CASTG 2 cut(s) 588, 993
VpaK11BI GGWCC 2 cut(s) 239, 427
XagI CCTNNNNNAGG 1 cut(s) 1216
XapI RAATTY 1 cut(s) 945
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XhoI CTCGAG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 2 cut(s) 533, 1276
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.