pycom10g13620

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006975
Physical Location & Seq
Reverse (-)
17105135 .. 17105491
357 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g13620.1

Sequence Viewer

Length: 357 bp
ATGGGTTCAAGAATCAGACCTCACAAACCCAAATACCTCCAAGCCATTCTTAAGGAAACCTTACGCCTATACCCACCTGGCCAAATCACAGGCCTCTGTGAGGCCACGAAGGACTGTCGTCTTGGTGGCTACTTCGTCCCCAAGCGCACTCATTTGCTAGTCAACATTTGGAAGTTGCAGCGGGACCCACGCATGTGGGCCAACCCTTGTGAGTTTCAACCTGAGAGGTTCATGATCACTCATGCTGACGTGGATTTCAAAGGTCAAAGTAATTTCAAGTATATTCCATTTAGTGCAGGTAGAGGTTTTTCTTGCTCCACGCCTTCCTCTTCAGCTTTACCAATGCCTTTGAAGTAA

Protein Analysis

119

Amino Acids

13.51

Weight (kDa)

9.86

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 11 - 101 7.7e-26 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
AciI CCGC 1 cut(s) 181
AcoI YGGCCR 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 100
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 5 cut(s) 9, 218, 259, 277, 352
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 76
AleI CACNNNNGTG 1 cut(s) 193
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
AoxI GGCC 4 cut(s) 79, 91, 102, 198
ApeKI GCWGC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 184, 198
AvaII GGWCC 1 cut(s) 184
BalI TGGCCA 1 cut(s) 81
BbvI GCAGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 78
BclI TGATCA 1 cut(s) 234
BfaI CTAG 1 cut(s) 158
BfrI CTTAAG 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 78
Bme18I GGWCC 1 cut(s) 184
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 184, 198
BmiI GGNNCC 2 cut(s) 185, 186
BmrFI CCNGG 1 cut(s) 78
BoxI GACNNNNGTC 1 cut(s) 117
Bsc4I CCNNNNNNNGG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 315
BshFI GGCC 4 cut(s) 81, 93, 104, 200
BslFI GGGAC 2 cut(s) 122, 197
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 2 cut(s) 122, 197
BsnI GGCC 4 cut(s) 81, 93, 104, 200
Bsp143I GATC 1 cut(s) 234
BspACI CCGC 1 cut(s) 181
BspANI GGCC 4 cut(s) 81, 93, 104, 200
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 185, 186
BspMI ACCTGC 1 cut(s) 287
BspTI CTTAAG 1 cut(s) 50
BssMI GATC 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 1 cut(s) 334
BstAFI CTTAAG 1 cut(s) 50
BstDEI CTNAG 1 cut(s) 222
BstENI CCTNNNNNAGG 1 cut(s) 98
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 237
BstMBI GATC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 196
BstPAI GACNNNNGTC 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 195
BsuRI GGCC 4 cut(s) 81, 93, 104, 200
BtrI CACGTC 1 cut(s) 250
BveI ACCTGC 1 cut(s) 287
CciI TCATGA 1 cut(s) 231
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 184, 198
CviAII CATG 3 cut(s) 193, 232, 242
CviJI RGCY 7 cut(s) 44, 81, 93, 104, 129, 200, 335
CviKI_1 RGCY 7 cut(s) 44, 81, 93, 104, 129, 200, 335
DdeI CTNAG 1 cut(s) 222
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
Eco147I AGGCCT 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 76
FaeI CATG 3 cut(s) 196, 235, 245
FaiI YATR 5 cut(s) 70, 194, 233, 243, 282
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 44, 76
FaqI GGGAC 2 cut(s) 122, 197
FatI CATG 3 cut(s) 192, 231, 241
FauI CCCGC 1 cut(s) 174
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 179
Fsp4HI GCNGC 1 cut(s) 179
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 179
HaeIII GGCC 4 cut(s) 81, 93, 104, 200
HhaI GCGC 1 cut(s) 147
Hin1II CATG 3 cut(s) 196, 235, 245
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 2 cut(s) 9, 232
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 103, 333
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 178, 296
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 3 cut(s) 196, 235, 245
HspAI GCGC 1 cut(s) 145
KflI GGGWCCC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 5 cut(s) 63, 75, 90, 234, 282
Lsp1109I GCAGC 1 cut(s) 190
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 249
MalI GATC 1 cut(s) 236
MboI GATC 1 cut(s) 234
MboII GAAGA 1 cut(s) 321
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 1 cut(s) 271
MluNI TGGCCA 1 cut(s) 81
MnlI CCTC 7 cut(s) 30, 47, 94, 104, 219, 296, 337
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 193
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 181
MspCI CTTAAG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
NdeII GATC 1 cut(s) 234
NlaIII CATG 3 cut(s) 196, 235, 245
NlaIV GGNNCC 2 cut(s) 185, 186
NspI RCATGY 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 193
PagI TCATGA 1 cut(s) 231
PceI AGGCCT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 12
PkrI GCNGC 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 185, 186
PspPI GGNCC 2 cut(s) 184, 198
PspPPI RGGWCCY 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 193
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 1 cut(s) 179
Sau3AI GATC 1 cut(s) 234
Sau96I GGNCC 2 cut(s) 184, 198
ScrFI CCNGG 1 cut(s) 78
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 271
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 1 cut(s) 181
SspMI CTAG 1 cut(s) 158
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 252
TasI AATT 1 cut(s) 271
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseI GCWGC 1 cut(s) 178
TspDTI ATGAA 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 184
XagI CCTNNNNNAGG 1 cut(s) 98
XceI RCATGY 1 cut(s) 196
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.