pycom05g17500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006975
Physical Location & Seq
Reverse (-)
20635777 .. 20636436
660 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17500.1

Sequence Viewer

Length: 660 bp
ATGGATTCAAATAAAGCCACTTTAAAACCTATATGTACACAGTACACATACGAAATCTATGATGTGATCATCCTACGTACATTTCATATATCTCCAACCATGGATGATTTCATTTCTCCAATCCACAAAATTATTAACACAATATTAGCTTTGCTTCTGGTTCTCTGTTGTGCATTGAGAATAATCGCAGCATACACTAAGCAAAACAAGAAGAAGAATATCATCAACAAGACATTGACCAGATCGTTGACCAAAGTCCCTCAACCCTCAGGAGCATGGCCTTTCATAGGTCACCTTCATCTCCTACATGGTAAAGATCCAGTTGCCCTAATCCTTGGCGCCATGGCCGATAAATTCGGTCCGATCTACTCACTTAAGCTTGGTCCGCGCCAAGTAATGGTGGTGCTTAGCGCTTGGGAACAAGTAAAAGAACGCTTCACAAAAAACGACATTGCTTTCTCCACACGACCGAGTACAGCAGCCGGCAAACACTTGGGTTACAACAACGCCATTTTTGCCCTAGCCCCGTACGGACCCTACTGGCGCGACATCCGTAAGTTGGCTACTCAGGAGTATCTCTCAGCCCAAAAAGTTGAAATGCTTAGTCACGTGCGAGCTGAAATAACCATAAGATTAAAAGATAATAAGAAAAACTCATAA

Protein Analysis

220

Amino Acids

24.76

Weight (kDa)

9.81

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 87 - 213 3.3e-14 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 397
AccII CGCG 2 cut(s) 388, 546
AciI CCGC 1 cut(s) 386
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 353
AcvI CACGTG 1 cut(s) 610
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 5 cut(s) 37, 44, 79, 475, 530
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 3 cut(s) 287, 397, 559
AflII CTTAAG 1 cut(s) 374
AgsI TTSAA 2 cut(s) 9, 596
AluBI AGCT 3 cut(s) 149, 379, 617
AluI AGCT 3 cut(s) 149, 379, 617
AlwI GGATC 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 2 cut(s) 278, 345
ApeKI GCWGC 2 cut(s) 188, 479
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 4 cut(s) 341, 390, 413, 546
AspS9I GGNCC 3 cut(s) 359, 383, 533
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 3 cut(s) 359, 383, 533
AxyI CCTNAGG 1 cut(s) 268
BanI GGYRCC 1 cut(s) 338
BbrPI CACGTG 1 cut(s) 610
BbvI GCAGC 2 cut(s) 200, 491
BclI TGATCA 1 cut(s) 66
BfaI CTAG 1 cut(s) 521
BfoI RGCGCY 2 cut(s) 342, 414
BfrI CTTAAG 1 cut(s) 374
BisI GCNGC 2 cut(s) 189, 480
BlpI GCTNAGC 1 cut(s) 407
BlsI GCNGC 2 cut(s) 190, 481
Bme18I GGWCC 3 cut(s) 359, 383, 533
BmgT120I GGNCC 3 cut(s) 359, 383, 533
BmiI GGNNCC 2 cut(s) 340, 535
BoxI GACNNNNGTC 1 cut(s) 254
BplI GAGNNNNNCTC 2 cut(s) 563, 595
Bpu1102I GCTNAGC 1 cut(s) 407
BsaAI YACGTR 2 cut(s) 77, 610
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 99, 334, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 397, 559
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 320, 545
Bse21I CCTNAGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 450
BseDI CCNNGG 3 cut(s) 99, 334, 342
BseGI GGATG 3 cut(s) 69, 109, 549
BseLI CCNNNNNNNGG 3 cut(s) 287, 397, 559
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 3 cut(s) 282, 581, 594
BseNI ACTGG 2 cut(s) 320, 545
BseXI GCAGC 2 cut(s) 200, 491
Bsh1236I CGCG 2 cut(s) 388, 546
Bsh1285I CGRYCG 1 cut(s) 470
BshFI GGCC 2 cut(s) 280, 347
BshNI GGYRCC 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 470
BsiSI CCGG 1 cut(s) 483
BsiWI CGTACG 1 cut(s) 528
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 3 cut(s) 287, 397, 559
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 2 cut(s) 280, 347
Bsp1407I TGTACA 1 cut(s) 35
Bsp143I GATC 4 cut(s) 66, 242, 316, 363
Bsp1720I GCTNAGC 1 cut(s) 407
Bsp19I CCATGG 2 cut(s) 99, 342
BspACI CCGC 1 cut(s) 386
BspANI GGCC 2 cut(s) 280, 347
BspCNI CTCAG 3 cut(s) 281, 580, 593
BspFNI CGCG 2 cut(s) 388, 546
BspLI GGNNCC 2 cut(s) 340, 535
BspPI GGATC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 482
BsrGI TGTACA 1 cut(s) 35
BsrI ACTGG 2 cut(s) 320, 545
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 3 cut(s) 99, 334, 342
BssMI GATC 4 cut(s) 66, 242, 316, 363
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 3 cut(s) 99, 334, 342
Bst4CI ACNGT 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 374
BstAUI TGTACA 1 cut(s) 35
BstBAI YACGTR 2 cut(s) 77, 610
BstC8I GCNNGC 2 cut(s) 484, 615
BstDEI CTNAG 6 cut(s) 198, 268, 407, 567, 580, 602
BstDSI CCRYGG 2 cut(s) 99, 342
BstEII GGTNACC 1 cut(s) 290
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 3 cut(s) 69, 109, 549
BstFNI CGCG 2 cut(s) 388, 546
BstH2I RGCGCY 2 cut(s) 342, 414
BstHHI GCGC 4 cut(s) 341, 390, 413, 546
BstKTI GATC 4 cut(s) 69, 245, 319, 366
BstMBI GATC 4 cut(s) 66, 242, 316, 363
BstMCI CGRYCG 1 cut(s) 470
BstMWI GCNNNNNNNGC 2 cut(s) 385, 515
BstPAI GACNNNNGTC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 290
BstSNI TACGTA 1 cut(s) 77
BstUI CGCG 2 cut(s) 388, 546
BstV1I GCAGC 2 cut(s) 200, 491
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 268
BsuRI GGCC 2 cut(s) 280, 347
BtgI CCRYGG 2 cut(s) 99, 342
BtsCI GGATG 3 cut(s) 69, 109, 549
Cac8I GCNNGC 2 cut(s) 484, 615
CfoI GCGC 4 cut(s) 341, 390, 413, 546
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 3 cut(s) 359, 383, 533
CpoI CGGWCCG 1 cut(s) 359
Csp6I GTAC 5 cut(s) 36, 43, 78, 474, 529
CspI CGGWCCG 1 cut(s) 359
CviAII CATG 4 cut(s) 100, 276, 308, 343
CviQI GTAC 5 cut(s) 36, 43, 78, 474, 529
DdeI CTNAG 6 cut(s) 198, 268, 407, 567, 580, 602
DinI GGCGCC 1 cut(s) 340
DpnI GATC 4 cut(s) 68, 244, 318, 365
DpnII GATC 4 cut(s) 66, 242, 316, 363
DraI TTTAAA 1 cut(s) 24
EaeI YGGCCR 1 cut(s) 345
Eco105I TACGTA 1 cut(s) 77
Eco130I CCWWGG 3 cut(s) 99, 334, 342
Eco47I GGWCC 3 cut(s) 359, 383, 533
Eco47III AGCGCT 1 cut(s) 412
Eco72I CACGTG 1 cut(s) 610
Eco81I CCTNAGG 1 cut(s) 268
Eco91I GGTNACC 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO65I GGTNACC 1 cut(s) 290
EcoT14I CCWWGG 3 cut(s) 99, 334, 342
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 99, 334, 342
FaeI CATG 4 cut(s) 103, 279, 311, 346
FaqI GGGAC 1 cut(s) 242
FatI CATG 4 cut(s) 99, 275, 307, 342
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 189, 480
FokI GGATG 3 cut(s) 56, 116, 536
Fsp4HI GCNGC 2 cut(s) 189, 480
FspBI CTAG 1 cut(s) 521
GlaI GCGC 4 cut(s) 340, 389, 412, 545
GluI GCNGC 2 cut(s) 189, 480
HaeII RGCGCY 2 cut(s) 342, 414
HaeIII GGCC 2 cut(s) 280, 347
HapII CCGG 1 cut(s) 483
HhaI GCGC 4 cut(s) 341, 390, 413, 546
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 4 cut(s) 103, 279, 311, 346
Hin6I GCGC 4 cut(s) 339, 388, 411, 544
HinP1I GCGC 4 cut(s) 339, 388, 411, 544
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 483
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 38, 45, 249
Hpy188I TCNGA 1 cut(s) 363
Hpy188III TCNNGA 2 cut(s) 270, 569
Hpy8I GTNNAC 3 cut(s) 38, 45, 249
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 76, 609
HpyCH4V TGCA 1 cut(s) 173
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 515
HpyF3I CTNAG 6 cut(s) 198, 268, 407, 567, 580, 602
HpySE526I ACGT 2 cut(s) 76, 609
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 4 cut(s) 103, 279, 311, 346
HspAI GCGC 4 cut(s) 339, 388, 411, 544
KasI GGCGCC 1 cut(s) 338
KroI GCCGGC 1 cut(s) 482
KroNI GCCGGC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 4 cut(s) 66, 242, 316, 363
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 7 cut(s) 143, 253, 255, 333, 496, 526, 554
Lsp1109I GCAGC 2 cut(s) 200, 491
MaeI CTAG 1 cut(s) 521
MaeII ACGT 2 cut(s) 76, 609
MaeIII GTNAC 3 cut(s) 290, 497, 605
MalI GATC 4 cut(s) 68, 244, 318, 365
MboI GATC 4 cut(s) 66, 242, 316, 363
MboII GAAGA 2 cut(s) 223, 226
MflI RGATCY 1 cut(s) 316
MluCI AATT 2 cut(s) 129, 353
Mly113I GGCGCC 1 cut(s) 339
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 2 cut(s) 270, 277
MroNI GCCGGC 1 cut(s) 482
MseI TTAA 4 cut(s) 23, 135, 375, 635
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 1 cut(s) 483
MvnI CGCG 2 cut(s) 388, 546
MwoI GCNNNNNNNGC 2 cut(s) 385, 515
NaeI GCCGGC 1 cut(s) 484
NarI GGCGCC 1 cut(s) 339
NcoI CCATGG 2 cut(s) 99, 342
NdeII GATC 4 cut(s) 66, 242, 316, 363
NgoMIV GCCGGC 1 cut(s) 482
NlaIII CATG 4 cut(s) 103, 279, 311, 346
NlaIV GGNNCC 2 cut(s) 340, 535
NmuCI GTSAC 2 cut(s) 290, 605
PdiI GCCGGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 5
Pfl23II CGTACG 1 cut(s) 528
PflMI CCANNNNNTGG 1 cut(s) 397
PkrI GCNGC 2 cut(s) 190, 481
PluTI GGCGCC 1 cut(s) 342
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
Ppu21I YACGTR 2 cut(s) 77, 610
PshAI GACNNNNGTC 1 cut(s) 254
PspCI CACGTG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 290
PspLI CGTACG 1 cut(s) 528
PspN4I GGNNCC 2 cut(s) 340, 535
PspPI GGNCC 3 cut(s) 359, 383, 533
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 5 cut(s) 37, 44, 79, 475, 530
RsaNI GTAC 5 cut(s) 36, 43, 78, 474, 529
Rsr2I CGGWCCG 1 cut(s) 359
RsrII CGGWCCG 1 cut(s) 359
SaqAI TTAA 4 cut(s) 23, 135, 375, 635
SatI GCNGC 2 cut(s) 189, 480
Sau3AI GATC 4 cut(s) 66, 242, 316, 363
Sau96I GGNCC 3 cut(s) 359, 383, 533
SetI ASST 8 cut(s) 31, 79, 151, 292, 297, 381, 612, 619
SfoI GGCGCC 1 cut(s) 340
SinI GGWCC 3 cut(s) 359, 383, 533
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SnaBI TACGTA 1 cut(s) 77
Sse9I AATT 2 cut(s) 129, 353
SsiI CCGC 1 cut(s) 386
SspDI GGCGCC 1 cut(s) 338
SspI AATATT 1 cut(s) 144
SspMI CTAG 1 cut(s) 521
StyI CCWWGG 3 cut(s) 99, 334, 342
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 79, 612
TaqII GACCGA 2 cut(s) 347, 484
TasI AATT 2 cut(s) 129, 353
TatI WGTACW 3 cut(s) 35, 42, 473
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 4 cut(s) 23, 135, 375, 635
Tru9I TTAA 4 cut(s) 23, 135, 375, 635
TseFI GTSAC 2 cut(s) 290, 605
TseI GCWGC 2 cut(s) 188, 479
Tsp45I GTSAC 2 cut(s) 290, 605
TspDTI ATGAA 4 cut(s) 74, 100, 274, 287
TspGWI ACGGA 2 cut(s) 542, 546
Van91I CCANNNNNTGG 1 cut(s) 397
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 3 cut(s) 359, 383, 533
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 1 cut(s) 353
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.