MD05G1211500.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011424
Physical Location & Seq
Forward (+)
34275511 .. 34280076
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1211500.v1.1.491

Sequence Viewer

Length: 3318 bp
ATGGCTTGGCAGGTTAGTCATCTCCTACAAATGTTGTTCCACATAGCACTCATATTTTCTCTCACTTCTGCCTCCATTAACTCTTTAGACCAAGAGGCTCTTTCCCTTTTGGAGTTCAAAAGATCACTTTCTGATCCAAGCAACAATCTTGAAACCTGGAATTCAGCATATTCAAATCCTTGCAATTGGACCGGAGTTCGTTGTTCTGATTTCAAGGTAACTTCTATAAATCTCAATGGACTTAATTTGTCTGGCACTTTGTCTCCAAGCATTTGTAATCTTCCTTACTTAACTGAATTCAACGTGTCCAAGAACTTCTTCTCTGGTCCTTTCCCTAAGGGTTTGGCAAATTGTCACAATCTTGAGATTCTAGACTTGTGCACAAATAGGTTTCATGGAGAAATGTTGACCCCTTTCTGCAAAATGACCAACCTTAGAAAGCTTAACCTCTGTGAGAATTATGTGTTTGGTGAAATGCCTGAGGAGATAGGGAATTTAGCTTCACTTGAAGAGCTTTTCATTTACAGTAATAATCTTACCGGTGTCATTCCCACATCTATTAGTAAGTTGAAAAGGCTCAAGGTCATCAGGGCGGGTAAAAACTCGCTTTCAGGGCCAATACCAACTGCTATTGGTGAGTGTGAGAGCTTAGAAGTTCTTGGGTTGGCACAGAATCAGTTAGAAGGATCGCTTCCGAGGGAGCTGCACAAGCTCCAAAATCTGACTGATTTGATCCTCTGGCAAAACCAATTAAGTGGTGAGATTCCTCCTGAGATTGGTAATATAAGTAGGCTGCAGTTGCTTGCCGTGCATGTCAATTCTTTCAGTGGATCGCCCCCGATAGAACTTGGGAGGTTATCCCAGCTCAAAAGATTGTATATCTATACCAACCAGCTGAACGGAACTATTCCAAGCGAGCTAGGGAATTGTACCAATGCTGTTGAGATAGACCTTTCTGAAAATCAGTTAAATGGACTCATCCCCCGGGAGTTGGGTTACATCCCTAATCTCCACCTGCTTCACCTTTTCGAGAACCTGCTTCAAGGAAATATTCCTAGAGAGCTTGGGCAGTTGAAGATGCTGCAGAGGTTAGATTTATCGATAAATCATTTGACAGGCACAATTCCATCAGAGTTTCAGAATCTCACTTGCATGGATGATTTGCAACTGTTTGATAATCATCTTGAGGGTAGAATTCCGCCTAACCTCGGAGCTAACAGTAACCTCACAATACTTGACATTTCTGCAAATAAGCTTGCTGGTAGAATACCTCCACACCTTTGTAAGTATCAGAGGTTAGTGTTTCTAAGCCTAGGTTCCAATAGGTTTTCCGGAAATATTCCATACGGTCTTAAGACATGCAAGTCTCTGATGCAGCTAATGCTAGGAGATAACCAGCTTACAGGAAGCCTCCCTATGGAGTTATATAACCTTTCTGCTCTTGAAGTCTATCAAAACCGGTTCTCAGGACCAATACCTCCAGAGGTAGGCAGGCTTACAAGTTTAGAAAGGCTGCTCTTGTCAGATAATTACTTTGTTGGGTACATTCCTCCTGAGATTGTGAATCTATCACAGCTTGTGACCTTCAACGTTTCCTCTAACAGACTCTCGGGTAGCATTCCTCATGAACTTGGAAATTTAACAAAATTACAAAGGCTTGATCTTAGTAGGAACCACTTCACTGGAAATCTCCCAGAAGAACTTGGAAAGCTAGTGAATCTAGAACTTTTGAAACTATCTGATAACAGTTTGATGGGAGGAATACCAGGGACTCTAGGGAGCCTGGCCCGACTGACGGAGTTGCAGATGGGAGGAAATCACTTTTCTGGTAGCATCCCTGTTGAGCTGGGCCAACTTATTGCTTTACAGATTTCCCTCAACATTAGTCATAACAATCTTTCGAGTACAATTCCTGAAAACTTGGGGAACTTGCAGATGTTGGAATCTCTCTACTTGAATGACAATCAGCTTGTTGGTGAAATTCCTGCCTCAATTGGTGAGTTGCTTAGCCTTTTGGTGTGCAACCTTTCTAACAATAACCTAGTGGGAACCGTGCCGAACACCTCTGTATTTCAAAGGATGGATTCTACGAATTTTGCAGGAAACAATGGCTTGTGCAGATCAGTTTCTAGTAGTTGTCACTCTGCAGCTCCATCTACTACTCCGAAGAGAAGCTGGATAAAGGACGGTTCATCTAGAGAGAAAATAGTGACCATTATATCCGTTATAATTGGATTAATCTCCTTGTCTTTTATAGTGGGTTTCTGTTGGGCAATGAATTGCCGAAAGCCTACTTTTGTTTCACTTGACGATCACACAAAGCCTGCAGAAGTGTTCGATAACTATTACTTTCCTAAGGAAGGTTTCAAATACCAAGACCTTGTTGAAGCTACCAGCAGTTTTTCAGACAGTGCAATTCTTGGAAAAGGAGCCTGTGGTATTGTGTACAAAGCTGTAATGTCTGACGGTGAGGTCATTGCGGTAAAGAAGCTAAAGGCACAAGGAGAAGGAGTAAGTGTTGATAGCAGCTTCCGCGCTGAGATATCAACCCTTGGCAAGATCAGGCATTGTAACATTGTGAAGCTCTATGGCTTCTGCTGCCACCAAGACTCAAATCTTCTCTTGTATGAGTACATGGAAAATGGAAGTCTAGGAGAACATCTCCATGGAAATGAACAAAGATGTTTTCTAGACTGGAACGCTCGATATAAGATTGCTCTTGGAGCAGCAGAGGGTTTGTGCTATCTTCATTACGACTGTAAGCCCCAAATCATCCACCGCGACATAAAGTCAAATAACATTTTATTGGATCAAGTTCTTCAAGCACACGTAGGAGACTTTGGCTTGGCTAAATTGATTGAGCTCCCATACTCAAAATCCATGTCCGCTGTTGCAGGTTCATATGGCTACATTGCCCCAGAGTATGCATACACGATGAAAGTGACTGAGAAATGTGACATATATAGCTTTGGGGTTGTACTGCTGGAACTAGTAACCGGGAAGTCACCTGTTCAACGGCTGGAGCTGGGAGGAGACCTCGTCACTTGGGTGAGAAGGGCAATACATAATGGAGTGGTAACAGCTGAAATATTTGACAAGAGGGTAGATCTGACAGTGAAAAGGACAGTTGAGGAAATGACTCTATTTCTCAAGATTGCATTGTTCTGCACTAGTACATCCCCAGTTAACAGGCCAACAATGAGGGAGGTGATCGCAATGATGATTGATGCTAGGGCGTCTGTGAGCAATTTCTCATCATCACCGACATCCGAGAGTCCTTTAGATGAAGGCACTTCAAGAGATTCCATGGGATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1106

Amino Acids

121.89

Weight (kDa)

5.92

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 69 4.6e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 115 - 245 4.5e-15 Leucine-rich repeat region
LRR_8 PF13855 143 - 203 7.2e-06 Leucine rich repeat
LRR_14 PF23598 490 - 606 2.8e-11 Leucine-rich repeat region
LRR_8 PF13855 501 - 560 8e-07 Leucine rich repeat
LRR_14 PF23598 544 - 623 1.9e-06 Leucine-rich repeat region
Pkinase PF00069 803 - 1071 5.7e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 806 - 1072 6.2e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17230 AT1G17230 AT1G17230
fragaria_vesca FvH4_3g16200 FvH4_3g16200 FvH4_3g16200
malus_domestica MD05G1211500.v1.1
prunus_persica Prupe.4G145700_v2.0.a1
pyrus_communis pycom05g19650
rosa_chinensis RchiOBHm_Chr5g0027271
rosa_laevigata RLG00000033007
rosa_multiflora Rmu_sc0001503.1_g000006
rosa_roxburghii Rroxscaffold_1G00052220 Rroxscaffold_4G00329160
rosa_rugosa Rorug05G0098000
rosa_samantha Rh5AG192100 Rh5BG189800 Rh5CG210200 Rh5DG191800
rosa_wichuraiana Rw5G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2228
AarI CACCTGC 1 cut(s) 1023
AasI GACNNNNNNGTC 2 cut(s) 1363, 2471
Acc36I ACCTGC 3 cut(s) 1023, 1044, 2885
AccII CGCG 2 cut(s) 2535, 2781
AccIII TCCGGA 1 cut(s) 1331
AciI CCGC 6 cut(s) 593, 1199, 2480, 2533, 2779, 2886
AclI AACGTT 1 cut(s) 1590
AclWI GGATC 5 cut(s) 128, 694, 727, 838, 2817
AcsI RAATTY 7 cut(s) 160, 296, 493, 1194, 1636, 1980, 2092
AcyI GRCGYC 1 cut(s) 3236
AfaI GTAC 7 cut(s) 931, 1544, 1906, 2447, 2633, 2979, 3175
AfiI CCNNNNNNNGG 7 cut(s) 776, 991, 1208, 1417, 1487, 1795, 2360
AflII CTTAAG 1 cut(s) 1352
AflIII ACRYGT 2 cut(s) 303, 2827
AgeI ACCGGT 2 cut(s) 539, 1458
AhdI GACNNNNNGTC 1 cut(s) 2788
AhlI ACTAGT 2 cut(s) 2989, 3170
AjnI CCWGG 3 cut(s) 155, 1765, 1782
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AleI CACNNNNGTG 1 cut(s) 3047
Alw21I GWGCWC 2 cut(s) 383, 2865
Alw26I GTCTC 4 cut(s) 267, 1371, 2829, 3027
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 5 cut(s) 128, 694, 727, 838, 2817
Ama87I CYCGRG 2 cut(s) 984, 1609
Aor13HI TCCGGA 1 cut(s) 1331
AoxI GGCC 4 cut(s) 614, 1785, 1849, 3191
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 9 cut(s) 703, 793, 1081, 1375, 1513, 2147, 2526, 2598, 2726
ApoI RAATTY 7 cut(s) 160, 296, 493, 1194, 1636, 1980, 2092
ArsI GACNNNNNNTTYG 2 cut(s) 2867, 2899
AseI ATTAAT 1 cut(s) 2237
AsiGI ACCGGT 2 cut(s) 539, 1458
Asp700I GAANNNNTTC 2 cut(s) 317, 1676
AspA2I CCTAGG 1 cut(s) 1312
AspLEI GCGC 1 cut(s) 2537
AspS9I GGNCC 6 cut(s) 189, 326, 614, 1469, 1786, 1849
AsuC2I CCSGG 3 cut(s) 985, 986, 2998
AvaI CYCGRG 2 cut(s) 984, 1609
AvaII GGWCC 3 cut(s) 189, 326, 1469
AvrII CCTAGG 1 cut(s) 1312
AxyI CCTNAGG 3 cut(s) 336, 480, 2355
BaeGI GKGCMC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 2865
Bbv12I GWGCWC 2 cut(s) 383, 2865
BbvI GCAGC 9 cut(s) 690, 780, 1068, 1387, 1500, 2159, 2538, 2585, 2738
BccI CCATC 5 cut(s) 1135, 1747, 1801, 2074, 2161
BceAI ACGGC 2 cut(s) 791, 3032
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 3 cut(s) 157, 1767, 1784
BcnI CCSGG 3 cut(s) 985, 986, 2998
BcoDI GTCTC 4 cut(s) 267, 1371, 2829, 3027
BcuI ACTAGT 2 cut(s) 2989, 3170
BfmI CTRYAG 4 cut(s) 794, 1082, 2145, 2325
BfrI CTTAAG 1 cut(s) 1352
BfuAI ACCTGC 3 cut(s) 1023, 1044, 2885
BglII AGATCT 1 cut(s) 3106
BisI GCNGC 9 cut(s) 704, 794, 1082, 1376, 1514, 2148, 2527, 2599, 2727
BlnI CCTAGG 1 cut(s) 1312
BlpI GCTNAGC 1 cut(s) 2006
BlsI GCNGC 9 cut(s) 705, 795, 1083, 1377, 1515, 2149, 2528, 2600, 2728
Bme1390I CCNGG 6 cut(s) 157, 985, 986, 1767, 1784, 2998
Bme18I GGWCC 3 cut(s) 189, 326, 1469
BmeRI GACNNNNNGTC 1 cut(s) 2788
BmeT110I CYCGRG 2 cut(s) 984, 1609
BmgT120I GGNCC 6 cut(s) 189, 326, 614, 1469, 1786, 1849
BmiI GGNNCC 5 cut(s) 1318, 1673, 1781, 2050, 2431
BmrFI CCNGG 6 cut(s) 157, 985, 986, 1767, 1784, 2998
BmrI ACTGGG 1 cut(s) 3176
BmsI GCATC 4 cut(s) 1068, 1362, 1842, 3217
BmuI ACTGGG 1 cut(s) 3176
BplI GAGNNNNNCTC 4 cut(s) 2646, 2678, 3021, 3053
BpmI CTGGAG 2 cut(s) 1464, 3041
Bpu1102I GCTNAGC 1 cut(s) 2006
BpuEI CTTGAG 4 cut(s) 383, 563, 1205, 3134
BpuMI CCSGG 3 cut(s) 985, 986, 2998
Bsa29I ATCGAT 1 cut(s) 1100
BsaAI YACGTR 1 cut(s) 2830
BsaBI GATNNNNATC 1 cut(s) 1179
BsaHI GRCGYC 1 cut(s) 3236
BsaI GGTCTC 1 cut(s) 3027
BsaJI CCNNGG 9 cut(s) 695, 983, 984, 1207, 1312, 1766, 2551, 2665, 3306
BsaWI WCCGGW 4 cut(s) 191, 539, 1331, 1458
BsaXI ACNNNNNCTCC 8 cut(s) 247, 277, 2496, 2502, 2526, 2532, 3024, 3054
Bsc4I CCNNNNNNNGG 7 cut(s) 776, 991, 1208, 1417, 1487, 1795, 2360
Bse118I RCCGGY 2 cut(s) 539, 1458
Bse1I ACTGG 3 cut(s) 1687, 2699, 3182
Bse21I CCTNAGG 3 cut(s) 336, 480, 2355
Bse3DI GCAATG 4 cut(s) 2280, 2475, 2910, 3222
Bse8I GATNNNNATC 1 cut(s) 1179
BseAI TCCGGA 1 cut(s) 1331
BseBI CCWGG 3 cut(s) 157, 1767, 1784
BseCI ATCGAT 1 cut(s) 1100
BseDI CCNNGG 9 cut(s) 695, 983, 984, 1207, 1312, 1766, 2551, 2665, 3306
BseGI GGATG 8 cut(s) 978, 999, 1162, 1833, 2085, 2772, 3176, 3266
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 7 cut(s) 776, 991, 1208, 1417, 1487, 1795, 2360
BseMI GCAATG 4 cut(s) 2280, 2475, 2910, 3222
BseMII CTCAG 6 cut(s) 471, 762, 1479, 1545, 2529, 2937
BseNI ACTGG 3 cut(s) 1687, 2699, 3182
BseRI GAGGAG 2 cut(s) 497, 3045
BseSI GKGCMC 1 cut(s) 383
BseXI GCAGC 9 cut(s) 690, 780, 1068, 1387, 1500, 2159, 2538, 2585, 2738
BseYI CCCAGC 3 cut(s) 861, 1846, 3025
BsgI GTGCAG 3 cut(s) 689, 2137, 3151
Bsh1236I CGCG 2 cut(s) 2535, 2781
BshFI GGCC 4 cut(s) 616, 1787, 1851, 3193
BshTI ACCGGT 2 cut(s) 539, 1458
BshVI ATCGAT 1 cut(s) 1100
BsiHKAI GWGCWC 2 cut(s) 383, 2865
BsiHKCI CYCGRG 2 cut(s) 984, 1609
BsiSI CCGG 6 cut(s) 192, 540, 985, 1332, 1459, 2997
BslFI GGGAC 1 cut(s) 1783
BslI CCNNNNNNNGG 7 cut(s) 776, 991, 1208, 1417, 1487, 1795, 2360
BsmAI GTCTC 4 cut(s) 267, 1371, 2829, 3027
BsmFI GGGAC 1 cut(s) 1783
BsmI GAATGC 1 cut(s) 1617
BsnI GGCC 4 cut(s) 616, 1787, 1851, 3193
Bso31I GGTCTC 1 cut(s) 3027
BsoBI CYCGRG 2 cut(s) 984, 1609
Bsp1286I GDGCHC 2 cut(s) 383, 2865
Bsp13I TCCGGA 1 cut(s) 1331
Bsp1407I TGTACA 1 cut(s) 2445
Bsp1720I GCTNAGC 1 cut(s) 2006
Bsp19I CCATGG 2 cut(s) 2665, 3306
BspACI CCGC 6 cut(s) 593, 1199, 2480, 2533, 2779, 2886
BspANI GGCC 4 cut(s) 616, 1787, 1851, 3193
BspCNI CTCAG 6 cut(s) 472, 763, 1478, 1546, 2530, 2938
BspDI ATCGAT 1 cut(s) 1100
BspEI TCCGGA 1 cut(s) 1331
BspFNI CGCG 2 cut(s) 2535, 2781
BspHI TCATGA 1 cut(s) 1624
BspLI GGNNCC 5 cut(s) 1318, 1673, 1781, 2050, 2431
BspMAI CTGCAG 4 cut(s) 798, 1086, 2149, 2329
BspMI ACCTGC 3 cut(s) 1023, 1044, 2885
BspPI GGATC 5 cut(s) 128, 694, 727, 838, 2817
BspQI GCTCTTC 1 cut(s) 504
BspTI CTTAAG 1 cut(s) 1352
BspTNI GGTCTC 1 cut(s) 3027
BsrDI GCAATG 4 cut(s) 2280, 2475, 2910, 3222
BsrFI RCCGGY 2 cut(s) 539, 1458
BsrGI TGTACA 1 cut(s) 2445
BsrI ACTGG 3 cut(s) 1687, 2699, 3182
BssAI RCCGGY 2 cut(s) 539, 1458
BssECI CCNNGG 9 cut(s) 695, 983, 984, 1207, 1312, 1766, 2551, 2665, 3306
BssNI GRCGYC 1 cut(s) 3236
BssT1I CCWWGG 4 cut(s) 1312, 2551, 2665, 3306
Bst2UI CCWGG 3 cut(s) 157, 1767, 1784
Bst6I CTCTTC 2 cut(s) 504, 2162
BstACI GRCGYC 1 cut(s) 3236
BstAFI CTTAAG 1 cut(s) 1352
BstAPI GCANNNNNTGC 1 cut(s) 1381
BstAUI TGTACA 1 cut(s) 2445
BstBAI YACGTR 1 cut(s) 2830
BstC8I GCNNGC 5 cut(s) 804, 917, 1257, 1493, 2325
BstDSI CCRYGG 2 cut(s) 2665, 3306
BstENI CCTNNNNNAGG 1 cut(s) 2358
BstF5I GGATG 8 cut(s) 978, 999, 1162, 1833, 2085, 2772, 3176, 3266
BstFNI CGCG 2 cut(s) 2535, 2781
BstHHI GCGC 1 cut(s) 2537
BstMAI GTCTC 4 cut(s) 267, 1371, 2829, 3027
BstMWI GCNNNNNNNGC 8 cut(s) 613, 709, 799, 808, 1381, 2532, 2598, 2723
BstNI CCWGG 3 cut(s) 157, 1767, 1784
BstNSI RCATGY 2 cut(s) 815, 1362
BstSCI CCNGG 6 cut(s) 155, 983, 984, 1765, 1782, 2996
BstSFI CTRYAG 4 cut(s) 794, 1082, 2145, 2325
BstSLI GKGCMC 1 cut(s) 383
BstUI CGCG 2 cut(s) 2535, 2781
BstV1I GCAGC 9 cut(s) 690, 780, 1068, 1387, 1500, 2159, 2538, 2585, 2738
BstX2I RGATCY 1 cut(s) 3106
BstXI CCANNNNNNTGG 2 cut(s) 755, 1682
BstYI RGATCY 1 cut(s) 3106
Bsu15I ATCGAT 1 cut(s) 1100
Bsu36I CCTNAGG 3 cut(s) 336, 480, 2355
BsuRI GGCC 4 cut(s) 616, 1787, 1851, 3193
BsuTUI ATCGAT 1 cut(s) 1100
BtgI CCRYGG 2 cut(s) 2665, 3306
BtsCI GGATG 8 cut(s) 978, 999, 1162, 1833, 2085, 2772, 3176, 3266
BtsIMutI CAGTG 4 cut(s) 832, 1680, 2416, 3120
BveI ACCTGC 3 cut(s) 1023, 1044, 2885
Cac8I GCNNGC 5 cut(s) 804, 917, 1257, 1493, 2325
CciI TCATGA 1 cut(s) 1624
CfoI GCGC 1 cut(s) 2537
Cfr10I RCCGGY 2 cut(s) 539, 1458
Cfr13I GGNCC 6 cut(s) 189, 326, 614, 1469, 1786, 1849
Cfr9I CCCGGG 1 cut(s) 984
ClaI ATCGAT 1 cut(s) 1100
CseI GACGC 1 cut(s) 3225
Csp6I GTAC 7 cut(s) 930, 1543, 1905, 2446, 2632, 2978, 3174
CspAI ACCGGT 2 cut(s) 539, 1458
CviAII CATG 9 cut(s) 395, 812, 1153, 1359, 1625, 2635, 2666, 2881, 3307
CviQI GTAC 7 cut(s) 930, 1543, 1905, 2446, 2632, 2978, 3174
DrdI GACNNNNNNGTC 2 cut(s) 1363, 2471
DriI GACNNNNNGTC 1 cut(s) 2788
DseDI GACNNNNNNGTC 2 cut(s) 1363, 2471
Eam1104I CTCTTC 2 cut(s) 504, 2162
Eam1105I GACNNNNNGTC 1 cut(s) 2788
EarI CTCTTC 2 cut(s) 504, 2162
EciI GGCGGA 1 cut(s) 1188
Ecl136II GAGCTC 1 cut(s) 2863
Eco130I CCWWGG 4 cut(s) 1312, 2551, 2665, 3306
Eco24I GRGCYC 1 cut(s) 2865
Eco31I GGTCTC 1 cut(s) 3027
Eco32I GATATC 1 cut(s) 2544
Eco47I GGWCC 3 cut(s) 189, 326, 1469
Eco53kI GAGCTC 1 cut(s) 2863
Eco81I CCTNAGG 3 cut(s) 336, 480, 2355
Eco88I CYCGRG 2 cut(s) 984, 1609
EcoICRI GAGCTC 1 cut(s) 2863
EcoNI CCTNNNNNAGG 1 cut(s) 2358
EcoRI GAATTC 3 cut(s) 160, 296, 1194
EcoRII CCWGG 3 cut(s) 155, 1765, 1782
EcoRV GATATC 1 cut(s) 2544
EcoT14I CCWWGG 4 cut(s) 1312, 2551, 2665, 3306
EcoT22I ATGCAT 1 cut(s) 2929
EcoT38I GRGCYC 1 cut(s) 2865
ErhI CCWWGG 4 cut(s) 1312, 2551, 2665, 3306
FaeI CATG 9 cut(s) 398, 815, 1156, 1362, 1628, 2638, 2669, 2884, 3310
FalI AAGNNNNNCTT 6 cut(s) 84, 116, 302, 334, 675, 707
FaqI GGGAC 1 cut(s) 1783
FatI CATG 9 cut(s) 394, 811, 1152, 1358, 1624, 2634, 2665, 2880, 3306
FauI CCCGC 1 cut(s) 586
FauNDI CATATG 1 cut(s) 2902
Fnu4HI GCNGC 9 cut(s) 704, 794, 1082, 1376, 1514, 2148, 2527, 2599, 2727
FokI GGATG 8 cut(s) 965, 986, 1169, 1820, 2092, 2759, 3163, 3253
FriOI GRGCYC 1 cut(s) 2865
Fsp4HI GCNGC 9 cut(s) 704, 794, 1082, 1376, 1514, 2148, 2527, 2599, 2727
GlaI GCGC 1 cut(s) 2536
GluI GCNGC 9 cut(s) 704, 794, 1082, 1376, 1514, 2148, 2527, 2599, 2727
GsaI CCCAGC 3 cut(s) 865, 1850, 3029
GsuI CTGGAG 2 cut(s) 1464, 3041
HaeIII GGCC 4 cut(s) 616, 1787, 1851, 3193
HapII CCGG 6 cut(s) 192, 540, 985, 1332, 1459, 2997
HgaI GACGC 1 cut(s) 3225
HhaI GCGC 1 cut(s) 2537
Hin1I GRCGYC 1 cut(s) 3236
Hin1II CATG 9 cut(s) 398, 815, 1156, 1362, 1628, 2638, 2669, 2884, 3310
Hin6I GCGC 1 cut(s) 2535
HinP1I GCGC 1 cut(s) 2535
HincII GTYRAC 2 cut(s) 408, 3187
HindII GTYRAC 2 cut(s) 408, 3187
HindIII AAGCTT 2 cut(s) 440, 1253
HpaI GTTAAC 1 cut(s) 3187
HpaII CCGG 6 cut(s) 192, 540, 985, 1332, 1459, 2997
Hpy166II GTNNAC 4 cut(s) 381, 408, 2446, 3187
Hpy8I GTNNAC 4 cut(s) 381, 408, 2446, 3187
HpyAV CCTTC 6 cut(s) 677, 1594, 2354, 2501, 3048, 3281
HpyCH4IV ACGT 3 cut(s) 303, 1590, 2829
HpyF10VI GCNNNNNNNGC 8 cut(s) 613, 709, 799, 808, 1381, 2532, 2598, 2723
HpySE526I ACGT 3 cut(s) 303, 1590, 2829
Hsp92I GRCGYC 1 cut(s) 3236
Hsp92II CATG 9 cut(s) 398, 815, 1156, 1362, 1628, 2638, 2669, 2884, 3310
HspAI GCGC 1 cut(s) 2535
Kpn2I TCCGGA 1 cut(s) 1331
KspAI GTTAAC 1 cut(s) 3187
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 9 cut(s) 700, 717, 1211, 1779, 2155, 2429, 2723, 2868, 3022
Lsp1109I GCAGC 9 cut(s) 690, 780, 1068, 1387, 1500, 2159, 2538, 2585, 2738
LweI GCATC 4 cut(s) 1068, 1362, 1842, 3217
MaeII ACGT 3 cut(s) 303, 1590, 2829
MboII GAAGA 9 cut(s) 272, 310, 521, 1087, 1709, 2179, 2609, 2738, 2810
MfeI CAATTG 2 cut(s) 184, 1992
MflI RGATCY 1 cut(s) 3106
MhlI GDGCHC 2 cut(s) 383, 2865
MlyI GAGTC 6 cut(s) 969, 1599, 1765, 2603, 3133, 3283
MmeI TCCRAC 1 cut(s) 1920
Mph1103I ATGCAT 1 cut(s) 2929
MroI TCCGGA 1 cut(s) 1331
MroXI GAANNNNTTC 2 cut(s) 317, 1676
MslI CAYNNNNRTG 4 cut(s) 1151, 2664, 2670, 3047
MspA1I CMGCKG 3 cut(s) 895, 2888, 3083
MspCI CTTAAG 1 cut(s) 1352
MspI CCGG 6 cut(s) 192, 540, 985, 1332, 1459, 2997
MspR9I CCNGG 6 cut(s) 157, 985, 986, 1767, 1784, 2998
MunI CAATTG 2 cut(s) 184, 1992
Mva1269I GAATGC 1 cut(s) 1617
MvaI CCWGG 3 cut(s) 157, 1767, 1784
MvnI CGCG 2 cut(s) 2535, 2781
MwoI GCNNNNNNNGC 8 cut(s) 613, 709, 799, 808, 1381, 2532, 2598, 2723
NciI CCSGG 3 cut(s) 985, 986, 2998
NcoI CCATGG 2 cut(s) 2665, 3306
NdeI CATATG 1 cut(s) 2902
NlaIII CATG 9 cut(s) 398, 815, 1156, 1362, 1628, 2638, 2669, 2884, 3310
NlaIV GGNNCC 5 cut(s) 1318, 1673, 1781, 2050, 2431
NmuCI GTSAC 8 cut(s) 353, 1579, 2138, 2209, 2941, 2954, 3003, 3040
NsiI ATGCAT 1 cut(s) 2929
NspI RCATGY 2 cut(s) 815, 1362
OliI CACNNNNGTG 1 cut(s) 3047
PagI TCATGA 1 cut(s) 1624
PaqCI CACCTGC 1 cut(s) 1023
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 1 cut(s) 1617
PdmI GAANNNNTTC 2 cut(s) 317, 1676
PfeI GAWTC 9 cut(s) 367, 673, 763, 1141, 1564, 1717, 1943, 2084, 3302
PflFI GACNNNGTC 1 cut(s) 3038
PinAI ACCGGT 2 cut(s) 539, 1458
PkrI GCNGC 9 cut(s) 705, 795, 1083, 1377, 1515, 2149, 2528, 2600, 2728
PleI GAGTC 6 cut(s) 969, 1599, 1765, 2603, 3133, 3282
PpsI GAGTC 6 cut(s) 969, 1599, 1765, 2603, 3133, 3282
Ppu21I YACGTR 1 cut(s) 2830
PshBI ATTAAT 1 cut(s) 2237
PsiI TTATAA 1 cut(s) 2228
Psp124BI GAGCTC 1 cut(s) 2865
Psp1406I AACGTT 1 cut(s) 1590
Psp6I CCWGG 3 cut(s) 155, 1765, 1782
PspFI CCCAGC 3 cut(s) 861, 1846, 3025
PspGI CCWGG 3 cut(s) 155, 1765, 1782
PspN4I GGNNCC 5 cut(s) 1318, 1673, 1781, 2050, 2431
PspPI GGNCC 6 cut(s) 189, 326, 614, 1469, 1786, 1849
PstI CTGCAG 4 cut(s) 798, 1086, 2149, 2329
PsuI RGATCY 1 cut(s) 3106
PsyI GACNNNGTC 1 cut(s) 3038
PvuII CAGCTG 2 cut(s) 895, 3083
RsaI GTAC 7 cut(s) 931, 1544, 1906, 2447, 2633, 2979, 3175
RsaNI GTAC 7 cut(s) 930, 1543, 1905, 2446, 2632, 2978, 3174
RseI CAYNNNNRTG 4 cut(s) 1151, 2664, 2670, 3047
SacI GAGCTC 1 cut(s) 2865
SapI GCTCTTC 1 cut(s) 504
SatI GCNGC 9 cut(s) 704, 794, 1082, 1376, 1514, 2148, 2527, 2599, 2727
Sau96I GGNCC 6 cut(s) 189, 326, 614, 1469, 1786, 1849
SchI GAGTC 6 cut(s) 969, 1599, 1765, 2603, 3133, 3283
ScrFI CCNGG 6 cut(s) 157, 985, 986, 1767, 1784, 2998
SduI GDGCHC 2 cut(s) 383, 2865
SfaNI GCATC 4 cut(s) 1068, 1362, 1842, 3217
SfcI CTRYAG 4 cut(s) 794, 1082, 2145, 2325
SinI GGWCC 3 cut(s) 189, 326, 1469
SmaI CCCGGG 1 cut(s) 986
SmiMI CAYNNNNRTG 4 cut(s) 1151, 2664, 2670, 3047
SmlI CTYRAG 5 cut(s) 362, 578, 1184, 1352, 3149
SmoI CTYRAG 5 cut(s) 362, 578, 1184, 1352, 3149
SpeI ACTAGT 2 cut(s) 2989, 3170
SsiI CCGC 6 cut(s) 593, 1199, 2480, 2533, 2779, 2886
SspI AATATT 3 cut(s) 1051, 1339, 3090
SstI GAGCTC 1 cut(s) 2865
StyD4I CCNGG 6 cut(s) 155, 983, 984, 1765, 1782, 2996
StyI CCWWGG 4 cut(s) 1312, 2551, 2665, 3306
TaiI ACGT 3 cut(s) 306, 1593, 2832
TaqI TCGA 5 cut(s) 1029, 1100, 1901, 2337, 2704
TatI WGTACW 5 cut(s) 1904, 2445, 2631, 2977, 3173
TfiI GAWTC 9 cut(s) 367, 673, 763, 1141, 1564, 1717, 1943, 2084, 3302
TscAI CASTG 4 cut(s) 832, 1687, 2416, 3120
TseFI GTSAC 8 cut(s) 353, 1579, 2138, 2209, 2941, 2954, 3003, 3040
TseI GCWGC 9 cut(s) 703, 793, 1081, 1375, 1513, 2147, 2526, 2598, 2726
Tsp45I GTSAC 8 cut(s) 353, 1579, 2138, 2209, 2941, 2954, 3003, 3040
TspGWI ACGGA 3 cut(s) 915, 1811, 2212
TspMI CCCGGG 1 cut(s) 984
TspRI CASTG 4 cut(s) 832, 1687, 2416, 3120
Tth111I GACNNNGTC 1 cut(s) 3038
Vha464I CTTAAG 1 cut(s) 1352
VneI GTGCAC 1 cut(s) 379
VpaK11BI GGWCC 3 cut(s) 189, 326, 1469
VspI ATTAAT 1 cut(s) 2237
XagI CCTNNNNNAGG 1 cut(s) 2358
XapI RAATTY 7 cut(s) 160, 296, 493, 1194, 1636, 1980, 2092
XbaI TCTAGA 4 cut(s) 370, 1720, 2195, 2689
XceI RCATGY 2 cut(s) 815, 1362
XmaI CCCGGG 1 cut(s) 984
XmaJI CCTAGG 1 cut(s) 1312
XmnI GAANNNNTTC 2 cut(s) 317, 1676
Zsp2I ATGCAT 1 cut(s) 2929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.