RLG00000033007
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011424
Physical Location & Seq
Reverse (-)
22149735 .. 22153289
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033007

Sequence Viewer

Length: 3282 bp
ATGCTATTACACTTGGGACTCCTCGTGTTATGTTTCAGTTTTGGCTCTACAAACTCATTAGATGAAGAAAGTCTTTTTCTTTTGGAGTTCAAGAGATCCCTATCTGATCGAAGTAACAATCTTGGAAGCTGGAATTCATCACATTTGAGTCCTTGCAATTGGACTGGTGTGAGGTGTCTCAAGTCCAAGGTAACTTCTATAAATCTCACTGGCCTTAATTTGTCTGGTGTTTTGTCTCCAGTCATTTGCAATCTTCCTTACTTGGTTGAATTCAATGTGTCCAAGAACTTCTTCTCTGGGCCATTTCCCAATGATCTTGCTAACTGTCATAATCTAGCGTTTCTAGACCTGTGCACAAATAGGTTTCATGGGGAATTGGTTACCCCATTCACCAAAATGACCAACCTTAGGAAGTTGTATCTCTGTGAGAATTATGTGTTTGGTGAAATGCCTGAGGAGATAGGGAACTTAACTTTACTTGAAGAGCTTGTGATTTATAGTAATAATCTCACTGGCTCCATTCCTGGTTCAATTAGTAAGTTGAAAAAGTTAAAAGTCCTCAGGGCGGGTCGAAATTCTCTTTCTGGCCCAATACCAACTGTTATCAGTCAGTGTCAGAGCTTAGAAGTTTTGGGGTTATCACAGAATCATTTAGAAGGTTCAATTCCCAGGGAGCTTGAAGAACTTCAAAATCTGACTGATTTGATCCTTTGGCAAAATCATTTAACTGGTTCAGTTCCTGCTGAGATTGGTAATCTAAGTAGGCTAGAGTTGCTTGCCTTGCATGAAAACTCTTTCGGAGGAATGATCCCTAAGGAACTTGGGAAGTTATCACAGCTGAAGAGATTGTATATATACACCAACCAGTTGAATGGAACTATCCCAAGTGAACTAGGGAATTGTACCAATGCGGTGCATATAGATTTTTCTGAAAACCAGTTAAGTGGAGTCATACCACGGGAGTTGGGTTACATTCCCAATCTTGTGTTGCTTCACCTCTTCGAAAATCATCTGGAAGGAAACATTCCGAGGGAGCTTGGCCAGTTGAAGCAGCTGCAGAGGTTAGACTTGTCCATAAACAATTTGACTGGCCCAATCCCACTAGAATTTCAGAACCTTACTTACATGGATGAGTTGCAACTGTTTGATAATCATCTCGAGGGTAAAATTCCTCCTCTCCTTGGAGCTAACAGTTACCTCAGCATTCTTGACATGTCTGCAAATAAGCTTGTGGGTAGCATACCTGCGCATCTTTGCGAGTATGGGAAATTAGCATTTCTAAGCCTTGGGTCAAATAGGTTGTCTGGAAATATTCCTTATGGTATTAAAACATGCAAGTCTCTAGTCCAGCTAATGCTAGGAGACAACCATCTTACAGGAAGTCTCCCTATGGAGTTATATACTCTTTATGCTCTTGAAGTCTTTCAAAACCAATTCTCAGGACCTATACCTCCAGAGGTAGGCAGGTTTAAAAGTTTAGAAAGGCTGCTGTTGTCAGATAACTATTTTATTGGGCATATTCCTCTTGAGATTGGCAACCTATCACAGCTTGTGACCTTCAATGTTTCCTCTAACAGGCTCACGGGGAACATTCCTCATGAGCTGGGAAATTGTACAAAGCTACAGAGGCTTGATCTCAGTAGGAATTACTTCACTGGAGTTCTCCCGGAAGAACTTGGAAAGCTAGTGAATCTGGAACTTTTGAAGCTTTCTGATAACAAGTTGATGGGAGGAATACCGAGTACTCTAGGGGACCTTGCCCGATTGACTGAGTTGGAGATGGGGGGAAATCACTTTTCTGGTAGCATCCCTTTTCAGCTGGGCCAACTTGGTGCTCTACAGATTGCCCTCAATATTAGCCATAATAATCTTTCGGGTGAAATTCCTGAAAAGTTGGGGGACTTGCAGATGTTGGAATCCCTCTACTTGAATGACAATCAGCTTGTTGGTGAAATTCCTGCCTCAATTGGTGAGTTGCTTAGCCTTCTGGTGTGCAACCTCTCTAACAATAACCTAGTGGGAACTGTGCCAAACACACAAGTATTTCAAAGGATGGATTCTTCAAATTTTGCTGGAAACAATGGTTTGTGCAGATCTGGATCATACCATTGTCATCAACCTCCAGTTCAATCTCATACTCCAAAACGGAGCTGGATAAAGGAGGGTTCATCTAAAGAGAAATTAGTAAGCATCATTTCTGCTGTAATTGGAGTAATTTCCTTGATTCTCATAGTTGGTTTCTGTTGGGCAATGAAGCGCTCCAGACCTACTTTTGTTCCACTTGAAGATCCAATCAAGCCAGACGTGTTGGACAACTATTACTTTCCGAAGGAAGGTTTCAAGTACCAGGATCTTGTTGTAGGCACCAACAATTTTTCAGACAATGCAGTTCTTGGAAGGGGAGCCTGTGGCACTGTGTACAAAGCTGTCATGGCTGATGGGGAGGTGATTGCAGTCAAAAAGCTCAAGGCACAAGGGGAAGGAGTCAGTGTCGATAGCAGCTTCCGCGCTGAGATATCAACCCTCGGGAAGATCAGACATTGCAATATTGTGAAGCTCTATGGCTTCTGCTGTCACCAAGACTCGAATCTGCTCTTGTATGAGTACATGGAAAATGGCAGTTTAGGAGAACATCTGCATGGAAACGATCAAAGATGTTATCTAGACTGGAATACACGATATAAGATTGCTCTTGGAGCAGCAGAGGGTTTGTGTTATCTCCATTATTACTGCAAGCCCCAAATCGTCCACCGTGACATTAAGTCAAATAACATTTTACTGGATGAAGTTCTTGAAGCACATGTGGGAGATTTCGGCTTAGCAAAATTGATTGAATTGCCATACTCAAAATCCATGTCTGCTGTTGCTGGCTCATATGGCTACATTGCACCAGAGTATGCTTACACAATGAAAGTGACTGAGAAGTGTGACATCTATAGTTTCGGAGTGGTTCTGCTGGAACTAGTAACCGGAAAGACACCCGTCCAACCACTGGAGCTGGGTGGAGACCTAGTCACTCTGGTAAGAAGAGCCATAAATAATTCAGTGGCAACATCCGAATTGTTTGACAAGAGGCTAGATCTGAGTCTGAGGAGCACAACTGAAGAGATGACTCTATTTCTCAAGATTGCGTTGTTCTGCACCAGTGTGTCCCCAGTTAAAAGGCCAACAATGAGGGAGGTCATTGCGATGATGATTGATGCTAGGCGGTCCGTGACTGTGAGCAACTGCTCATCACCAACATCTGAGAGTCCTTTGGATAAAGATCCTTCAAGAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1094

Amino Acids

120.76

Weight (kDa)

5.97

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 59 8.5e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 105 - 235 1.2e-13 Leucine-rich repeat region
LRR_8 PF13855 134 - 194 8.2e-06 Leucine rich repeat
LRR_8 PF13855 492 - 550 8.3e-06 Leucine rich repeat
LRR_8 PF13855 540 - 599 4.2e-08 Leucine rich repeat
Pkinase PF00069 794 - 1062 1.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 796 - 1063 2.1e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17230 AT1G17230 AT1G17230
fragaria_vesca FvH4_3g16200 FvH4_3g16200 FvH4_3g16200
malus_domestica MD05G1211500.v1.1
prunus_persica Prupe.4G145700_v2.0.a1
pyrus_communis pycom05g19650
rosa_chinensis RchiOBHm_Chr5g0027271
rosa_laevigata RLG00000033007
rosa_multiflora Rmu_sc0001503.1_g000006
rosa_roxburghii Rroxscaffold_1G00052220 Rroxscaffold_4G00329160
rosa_rugosa Rorug05G0098000
rosa_samantha Rh5AG192100 Rh5BG189800 Rh5CG210200 Rh5DG191800
rosa_wichuraiana Rw5G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1248
Acc36I ACCTGC 2 cut(s) 1252, 1455
AccB1I GGYRCC 1 cut(s) 2363
AccB7I CCANNNNNTGG 1 cut(s) 2992
AccII CGCG 1 cut(s) 2508
AciI CCGC 4 cut(s) 566, 911, 2506, 3208
AclWI GGATC 7 cut(s) 90, 700, 802, 2107, 2282, 2358, 3260
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 8 cut(s) 133, 269, 574, 1106, 1167, 1881, 1953, 2065
AcuI CTGAAG 2 cut(s) 860, 3123
AfaI GTAC 6 cut(s) 904, 1615, 1744, 2345, 2420, 2606
AfeI AGCGCT 1 cut(s) 2258
AfiI CCNNNNNNNGG 8 cut(s) 408, 565, 1181, 1460, 1575, 2333, 2992, 3275
AflIII ACRYGT 3 cut(s) 1212, 2304, 2800
AhdI GACNNNNNGTC 1 cut(s) 2761
AhlI ACTAGT 1 cut(s) 2962
AjiI CACGTC 1 cut(s) 2305
AjnI CCWGG 3 cut(s) 523, 668, 2346
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AleI CACNNNNGTG 1 cut(s) 3146
Alw21I GWGCWC 3 cut(s) 356, 1837, 3098
Alw26I GTCTC 6 cut(s) 182, 240, 1344, 1356, 1388, 3000
Alw44I GTGCAC 1 cut(s) 352
AlwI GGATC 7 cut(s) 90, 700, 802, 2107, 2282, 2358, 3260
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 2 cut(s) 1157, 2525
Aor51HI AGCGCT 1 cut(s) 2258
AoxI GGCC 7 cut(s) 211, 299, 586, 1039, 1090, 1822, 3164
ApaLI GTGCAC 1 cut(s) 352
ApeKI GCWGC 5 cut(s) 1051, 1054, 1486, 2499, 2699
ApoI RAATTY 8 cut(s) 133, 269, 574, 1106, 1167, 1881, 1953, 2065
ArsI GACNNNNNNTTYG 4 cut(s) 1286, 1318, 2840, 2872
Asp700I GAANNNNTTC 4 cut(s) 290, 684, 1422, 1649
AspLEI GCGC 3 cut(s) 1249, 2259, 2510
AspS9I GGNCC 7 cut(s) 299, 587, 1091, 1442, 1753, 1822, 3210
AsuC2I CCSGG 1 cut(s) 1667
AsuHPI GGTGA 9 cut(s) 382, 455, 986, 1889, 1961, 1982, 2458, 2567, 3228
AsuII TTCGAA 1 cut(s) 1002
AvaI CYCGRG 2 cut(s) 1157, 2525
AvaII GGWCC 3 cut(s) 1442, 1753, 3210
AxyI CCTNAGG 4 cut(s) 407, 453, 560, 813
BaeGI GKGCMC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 1041
BanI GGYRCC 1 cut(s) 2363
BauI CACGAG 1 cut(s) 23
Bbv12I GWGCWC 3 cut(s) 356, 1837, 3098
BbvCI CCTCAGC 1 cut(s) 1199
BbvI GCAGC 5 cut(s) 1041, 1063, 1473, 2511, 2711
BccI CCATC 5 cut(s) 1377, 1720, 1774, 2047, 2432
BciT130I CCWGG 3 cut(s) 525, 670, 2348
BcnI CCSGG 1 cut(s) 1667
BcoDI GTCTC 6 cut(s) 182, 240, 1344, 1356, 1388, 3000
BcuI ACTAGT 1 cut(s) 2962
BfmI CTRYAG 4 cut(s) 1055, 1622, 1838, 2935
BfoI RGCGCY 1 cut(s) 2260
BfuAI ACCTGC 2 cut(s) 1252, 1455
BglII AGATCT 2 cut(s) 2093, 3079
BisI GCNGC 5 cut(s) 1052, 1055, 1487, 2500, 2700
BlpI GCTNAGC 2 cut(s) 1979, 2818
BlsI GCNGC 5 cut(s) 1053, 1056, 1488, 2501, 2701
BmcAI AGTACT 1 cut(s) 1744
Bme1390I CCNGG 4 cut(s) 525, 670, 1667, 2348
Bme18I GGWCC 3 cut(s) 1442, 1753, 3210
BmeRI GACNNNNNGTC 1 cut(s) 2761
BmeT110I CYCGRG 2 cut(s) 1157, 2525
BmgBI CACGTC 1 cut(s) 2305
BmgT120I GGNCC 7 cut(s) 299, 587, 1091, 1442, 1753, 1822, 3210
BmiI GGNNCC 4 cut(s) 517, 1754, 2365, 2404
BmrFI CCNGG 4 cut(s) 525, 670, 1667, 2348
BmrI ACTGGG 1 cut(s) 3149
BmsI GCATC 4 cut(s) 1258, 1815, 2199, 3190
BmuI ACTGGG 1 cut(s) 3149
BoxI GACNNNNGTC 1 cut(s) 2981
BpmI CTGGAG 6 cut(s) 222, 1437, 1677, 2106, 2245, 3014
Bpu10I CCTNAGC 1 cut(s) 1199
Bpu1102I GCTNAGC 2 cut(s) 1979, 2818
Bpu14I TTCGAA 1 cut(s) 1002
BpuEI CTTGAG 4 cut(s) 164, 1547, 2450, 3107
BpuMI CCSGG 1 cut(s) 1667
BsaBI GATNNNNATC 4 cut(s) 100, 1152, 2098, 3264
BsaI GGTCTC 1 cut(s) 3000
BsaJI CCNNGG 8 cut(s) 186, 668, 669, 956, 1028, 1180, 1285, 2524
BsaWI WCCGGW 1 cut(s) 2969
BsaXI ACNNNNNCTCC 4 cut(s) 2475, 2505, 2997, 3027
Bsc4I CCNNNNNNNGG 8 cut(s) 408, 565, 1181, 1460, 1575, 2333, 2992, 3275
Bse21I CCTNAGG 4 cut(s) 407, 453, 560, 813
Bse3DI GCAATG 4 cut(s) 2256, 2539, 2883, 3183
Bse8I GATNNNNATC 4 cut(s) 100, 1152, 2098, 3264
BseBI CCWGG 3 cut(s) 525, 670, 2348
BseDI CCNNGG 8 cut(s) 186, 668, 669, 956, 1028, 1180, 1285, 2524
BseGI GGATG 5 cut(s) 1135, 1806, 2058, 2788, 3053
BseJI GATNNNNATC 4 cut(s) 100, 1152, 2098, 3264
BseLI CCNNNNNNNGG 8 cut(s) 408, 565, 1181, 1460, 1575, 2333, 2992, 3275
BseMI GCAATG 4 cut(s) 2256, 2539, 2883, 3183
BseRI GAGGAG 4 cut(s) 11, 470, 1164, 3106
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 5 cut(s) 1041, 1063, 1473, 2511, 2711
BseYI CCCAGC 3 cut(s) 1603, 1819, 2998
BsgI GTGCAG 2 cut(s) 2110, 3124
Bsh1236I CGCG 1 cut(s) 2508
BshFI GGCC 7 cut(s) 213, 301, 588, 1041, 1092, 1824, 3166
BshNI GGYRCC 1 cut(s) 2363
BsiHKAI GWGCWC 3 cut(s) 356, 1837, 3098
BsiHKCI CYCGRG 2 cut(s) 1157, 2525
BsiSI CCGG 2 cut(s) 1667, 2970
BslFI GGGAC 4 cut(s) 30, 1766, 1913, 3136
BslI CCNNNNNNNGG 8 cut(s) 408, 565, 1181, 1460, 1575, 2333, 2992, 3275
BsmAI GTCTC 6 cut(s) 182, 240, 1344, 1356, 1388, 3000
BsmFI GGGAC 4 cut(s) 30, 1766, 1913, 3136
BsmI GAATGC 1 cut(s) 1203
BsnI GGCC 7 cut(s) 213, 301, 588, 1041, 1092, 1824, 3166
Bso31I GGTCTC 1 cut(s) 3000
BsoBI CYCGRG 2 cut(s) 1157, 2525
Bsp119I TTCGAA 1 cut(s) 1002
Bsp1286I GDGCHC 3 cut(s) 356, 1837, 3098
Bsp1407I TGTACA 2 cut(s) 1613, 2418
Bsp1720I GCTNAGC 2 cut(s) 1979, 2818
BspACI CCGC 4 cut(s) 566, 911, 2506, 3208
BspANI GGCC 7 cut(s) 213, 301, 588, 1041, 1092, 1824, 3166
BspFNI CGCG 1 cut(s) 2508
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 4 cut(s) 517, 1754, 2365, 2404
BspMAI CTGCAG 1 cut(s) 1059
BspMI ACCTGC 2 cut(s) 1252, 1455
BspPI GGATC 7 cut(s) 90, 700, 802, 2107, 2282, 2358, 3260
BspQI GCTCTTC 2 cut(s) 477, 3022
BspT104I TTCGAA 1 cut(s) 1002
BspT107I GGYRCC 1 cut(s) 2363
BspTNI GGTCTC 1 cut(s) 3000
BsrDI GCAATG 4 cut(s) 2256, 2539, 2883, 3183
BsrGI TGTACA 2 cut(s) 1613, 2418
BssECI CCNNGG 8 cut(s) 186, 668, 669, 956, 1028, 1180, 1285, 2524
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 186, 1180, 1285
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 525, 670, 2348
Bst4CI ACNGT 8 cut(s) 326, 601, 1143, 1193, 2026, 2416, 2753, 3220
Bst6I CTCTTC 5 cut(s) 477, 836, 1004, 3022, 3099
BstAUI TGTACA 2 cut(s) 1613, 2418
BstBI TTCGAA 1 cut(s) 1002
BstC8I GCNNGC 3 cut(s) 777, 2735, 2869
BstDSI CCRYGG 1 cut(s) 956
BstEII GGTNACC 1 cut(s) 379
BstENI CCTNNNNNAGG 2 cut(s) 1573, 3273
BstF5I GGATG 5 cut(s) 1135, 1806, 2058, 2788, 3053
BstFNI CGCG 1 cut(s) 2508
BstH2I RGCGCY 1 cut(s) 2260
BstHHI GCGC 3 cut(s) 1249, 2259, 2510
BstMAI GTCTC 6 cut(s) 182, 240, 1344, 1356, 1388, 3000
BstMWI GCNNNNNNNGC 7 cut(s) 772, 781, 1627, 2432, 2505, 2696, 2877
BstNI CCWGG 3 cut(s) 525, 670, 2348
BstNSI RCATGY 3 cut(s) 1216, 1335, 2804
BstPAI GACNNNNGTC 1 cut(s) 2981
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 4 cut(s) 523, 668, 1665, 2346
BstSFI CTRYAG 4 cut(s) 1055, 1622, 1838, 2935
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 1 cut(s) 2508
BstV1I GCAGC 5 cut(s) 1041, 1063, 1473, 2511, 2711
BstX2I RGATCY 6 cut(s) 95, 2093, 2287, 2350, 3079, 3265
BstXI CCANNNNNNTGG 2 cut(s) 872, 944
BstYI RGATCY 6 cut(s) 95, 2093, 2287, 2350, 3079, 3265
Bsu36I CCTNAGG 4 cut(s) 407, 453, 560, 813
BsuRI GGCC 7 cut(s) 213, 301, 588, 1041, 1092, 1824, 3166
BtgI CCRYGG 1 cut(s) 956
BtgZI GCGATG 1 cut(s) 3203
BtrI CACGTC 1 cut(s) 2305
BtsCI GGATG 5 cut(s) 1135, 1806, 2058, 2788, 3053
BtsIMutI CAGTG 9 cut(s) 207, 510, 617, 1653, 2412, 2494, 2990, 3051, 3151
BveI ACCTGC 2 cut(s) 1252, 1455
Cac8I GCNNGC 3 cut(s) 777, 2735, 2869
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 1 cut(s) 1597
CfoI GCGC 3 cut(s) 1249, 2259, 2510
Cfr13I GGNCC 7 cut(s) 299, 587, 1091, 1442, 1753, 1822, 3210
CpoI CGGWCCG 1 cut(s) 3210
Csp6I GTAC 6 cut(s) 903, 1614, 1743, 2344, 2419, 2605
CspCI CAANNNNNGTGG 2 cut(s) 945, 980
CspI CGGWCCG 1 cut(s) 3210
CviQI GTAC 6 cut(s) 903, 1614, 1743, 2344, 2419, 2605
DraI TTTAAA 1 cut(s) 1471
DriI GACNNNNNGTC 1 cut(s) 2761
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 5 cut(s) 477, 836, 1004, 3022, 3099
Eam1105I GACNNNNNGTC 1 cut(s) 2761
EarI CTCTTC 5 cut(s) 477, 836, 1004, 3022, 3099
Eco130I CCWWGG 3 cut(s) 186, 1180, 1285
Eco31I GGTCTC 1 cut(s) 3000
Eco32I GATATC 1 cut(s) 2517
Eco47I GGWCC 3 cut(s) 1442, 1753, 3210
Eco47III AGCGCT 1 cut(s) 2258
Eco57I CTGAAG 2 cut(s) 860, 3123
Eco81I CCTNAGG 4 cut(s) 407, 453, 560, 813
Eco88I CYCGRG 2 cut(s) 1157, 2525
Eco91I GGTNACC 1 cut(s) 379
EcoNI CCTNNNNNAGG 2 cut(s) 1573, 3273
EcoO109I RGGNCCY 2 cut(s) 1442, 1753
EcoO65I GGTNACC 1 cut(s) 379
EcoRI GAATTC 2 cut(s) 133, 269
EcoRII CCWGG 3 cut(s) 523, 668, 2346
EcoRV GATATC 1 cut(s) 2517
EcoT14I CCWWGG 3 cut(s) 186, 1180, 1285
ErhI CCWWGG 3 cut(s) 186, 1180, 1285
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 275, 307
FaqI GGGAC 4 cut(s) 30, 1766, 1913, 3136
FauI CCCGC 1 cut(s) 559
FauNDI CATATG 1 cut(s) 2875
Fnu4HI GCNGC 5 cut(s) 1052, 1055, 1487, 2500, 2700
FokI GGATG 5 cut(s) 1142, 1793, 2065, 2795, 3040
Fsp4HI GCNGC 5 cut(s) 1052, 1055, 1487, 2500, 2700
FspI TGCGCA 1 cut(s) 1248
GlaI GCGC 3 cut(s) 1248, 2258, 2509
GluI GCNGC 5 cut(s) 1052, 1055, 1487, 2500, 2700
GsaI CCCAGC 3 cut(s) 1607, 1823, 3002
GsuI CTGGAG 6 cut(s) 222, 1437, 1677, 2106, 2245, 3014
HaeII RGCGCY 1 cut(s) 2260
HaeIII GGCC 7 cut(s) 213, 301, 588, 1041, 1092, 1824, 3166
HapII CCGG 2 cut(s) 1667, 2970
HhaI GCGC 3 cut(s) 1249, 2259, 2510
Hin6I GCGC 3 cut(s) 1247, 2257, 2508
HinP1I GCGC 3 cut(s) 1247, 2257, 2508
HindIII AAGCTT 2 cut(s) 1226, 1706
HpaII CCGG 2 cut(s) 1667, 2970
HphI GGTGA 9 cut(s) 382, 455, 986, 1889, 1961, 1982, 2458, 2567, 3228
Hpy166II GTNNAC 4 cut(s) 354, 890, 2419, 2749
Hpy8I GTNNAC 4 cut(s) 354, 890, 2419, 2749
HpyAV CCTTC 9 cut(s) 650, 1010, 1567, 1994, 2323, 2327, 2391, 2474, 3279
HpyCH4III ACNGT 8 cut(s) 326, 601, 1143, 1193, 2026, 2416, 2753, 3220
HpyCH4IV ACGT 1 cut(s) 2304
HpyF10VI GCNNNNNNNGC 7 cut(s) 772, 781, 1627, 2432, 2505, 2696, 2877
HpySE526I ACGT 1 cut(s) 2304
HspAI GCGC 3 cut(s) 1247, 2257, 2508
LguI GCTCTTC 2 cut(s) 477, 3022
Lsp1109I GCAGC 5 cut(s) 1041, 1063, 1473, 2511, 2711
LweI GCATC 4 cut(s) 1258, 1815, 2199, 3190
MaeII ACGT 1 cut(s) 2304
MfeI CAATTG 2 cut(s) 157, 1965
MflI RGATCY 6 cut(s) 95, 2093, 2287, 2350, 3079, 3265
MhlI GDGCHC 3 cut(s) 356, 1837, 3098
MlsI TGGCCA 1 cut(s) 1041
MluNI TGGCCA 1 cut(s) 1041
MlyI GAGTC 8 cut(s) 12, 157, 957, 2493, 2576, 3094, 3106, 3259
MmeI TCCRAC 4 cut(s) 1755, 1893, 2289, 3010
Mox20I TGGCCA 1 cut(s) 1041
MroXI GAANNNNTTC 4 cut(s) 290, 684, 1422, 1649
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 9 cut(s) 216, 470, 551, 725, 941, 1326, 1470, 2760, 3159
MslI CAYNNNNRTG 4 cut(s) 395, 2637, 3146, 3188
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 3 cut(s) 838, 1054, 1819
MspI CCGG 2 cut(s) 1667, 2970
MspR9I CCNGG 4 cut(s) 525, 670, 1667, 2348
MunI CAATTG 2 cut(s) 157, 1965
Mva1269I GAATGC 1 cut(s) 1203
MvaI CCWGG 3 cut(s) 525, 670, 2348
MvnI CGCG 1 cut(s) 2508
MwoI GCNNNNNNNGC 7 cut(s) 772, 781, 1627, 2432, 2505, 2696, 2877
NciI CCSGG 1 cut(s) 1667
NdeI CATATG 1 cut(s) 2875
NlaIV GGNNCC 4 cut(s) 517, 1754, 2365, 2404
NmuCI GTSAC 7 cut(s) 1552, 2573, 2753, 2914, 2927, 3013, 3214
NsbI TGCGCA 1 cut(s) 1248
NspI RCATGY 3 cut(s) 1216, 1335, 2804
NspV TTCGAA 1 cut(s) 1002
OliI CACNNNNGTG 1 cut(s) 3146
PaeR7I CTCGAG 1 cut(s) 1157
PagI TCATGA 1 cut(s) 1597
PasI CCCWGGG 1 cut(s) 669
PciI ACATGT 2 cut(s) 1212, 2800
PciSI GCTCTTC 2 cut(s) 477, 3022
PctI GAATGC 1 cut(s) 1203
PdmI GAANNNNTTC 4 cut(s) 290, 684, 1422, 1649
PfeI GAWTC 6 cut(s) 646, 1690, 1916, 2057, 2224, 2587
PflFI GACNNNGTC 1 cut(s) 3011
PflMI CCANNNNNTGG 1 cut(s) 2992
PfoI TCCNGGA 1 cut(s) 1665
PkrI GCNGC 5 cut(s) 1053, 1056, 1488, 2501, 2701
PleI GAGTC 8 cut(s) 12, 156, 956, 2492, 2576, 3093, 3106, 3258
PpsI GAGTC 8 cut(s) 12, 156, 956, 2492, 2576, 3093, 3106, 3258
PpuMI RGGWCCY 2 cut(s) 1442, 1753
PscI ACATGT 2 cut(s) 1212, 2800
PshAI GACNNNNGTC 1 cut(s) 2981
Psp5II RGGWCCY 2 cut(s) 1442, 1753
Psp6I CCWGG 3 cut(s) 523, 668, 2346
PspEI GGTNACC 1 cut(s) 379
PspFI CCCAGC 3 cut(s) 1603, 1819, 2998
PspGI CCWGG 3 cut(s) 523, 668, 2346
PspN4I GGNNCC 4 cut(s) 517, 1754, 2365, 2404
PspPI GGNCC 7 cut(s) 299, 587, 1091, 1442, 1753, 1822, 3210
PspPPI RGGWCCY 2 cut(s) 1442, 1753
PsrI GAACNNNNNNTAC 2 cut(s) 1106, 1138
PstI CTGCAG 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 6 cut(s) 95, 2093, 2287, 2350, 3079, 3265
PsyI GACNNNGTC 1 cut(s) 3011
PvuII CAGCTG 3 cut(s) 838, 1054, 1819
RsaI GTAC 6 cut(s) 904, 1615, 1744, 2345, 2420, 2606
RsaNI GTAC 6 cut(s) 903, 1614, 1743, 2344, 2419, 2605
RseI CAYNNNNRTG 4 cut(s) 395, 2637, 3146, 3188
Rsr2I CGGWCCG 1 cut(s) 3210
RsrII CGGWCCG 1 cut(s) 3210
SapI GCTCTTC 2 cut(s) 477, 3022
SaqAI TTAA 9 cut(s) 216, 470, 551, 725, 941, 1326, 1470, 2760, 3159
SatI GCNGC 5 cut(s) 1052, 1055, 1487, 2500, 2700
Sau96I GGNCC 7 cut(s) 299, 587, 1091, 1442, 1753, 1822, 3210
ScaI AGTACT 1 cut(s) 1744
SchI GAGTC 8 cut(s) 12, 157, 957, 2493, 2576, 3094, 3106, 3259
ScrFI CCNGG 4 cut(s) 525, 670, 1667, 2348
SduI GDGCHC 3 cut(s) 356, 1837, 3098
SfaNI GCATC 4 cut(s) 1258, 1815, 2199, 3190
SfcI CTRYAG 4 cut(s) 1055, 1622, 1838, 2935
Sfr274I CTCGAG 1 cut(s) 1157
SfuI TTCGAA 1 cut(s) 1002
SinI GGWCC 3 cut(s) 1442, 1753, 3210
SlaI CTCGAG 1 cut(s) 1157
SmiMI CAYNNNNRTG 4 cut(s) 395, 2637, 3146, 3188
SmlI CTYRAG 5 cut(s) 179, 1157, 1526, 2465, 3122
SmoI CTYRAG 5 cut(s) 179, 1157, 1526, 2465, 3122
SpeI ACTAGT 1 cut(s) 2962
SsiI CCGC 4 cut(s) 566, 911, 2506, 3208
SspI AATATT 3 cut(s) 1312, 1855, 2548
StyD4I CCNGG 4 cut(s) 523, 668, 1665, 2346
StyI CCWWGG 3 cut(s) 186, 1180, 1285
TaaI ACNGT 8 cut(s) 326, 601, 1143, 1193, 2026, 2416, 2753, 3220
TaiI ACGT 1 cut(s) 2307
TaqI TCGA 6 cut(s) 109, 571, 1002, 1158, 2493, 2585
TatI WGTACW 4 cut(s) 1613, 1742, 2418, 2604
TfiI GAWTC 6 cut(s) 646, 1690, 1916, 2057, 2224, 2587
Tru1I TTAA 9 cut(s) 216, 470, 551, 725, 941, 1326, 1470, 2760, 3159
Tru9I TTAA 9 cut(s) 216, 470, 551, 725, 941, 1326, 1470, 2760, 3159
TscAI CASTG 9 cut(s) 214, 517, 617, 1660, 2419, 2494, 2997, 3051, 3151
TseFI GTSAC 7 cut(s) 1552, 2573, 2753, 2914, 2927, 3013, 3214
TseI GCWGC 5 cut(s) 1051, 1054, 1486, 2499, 2699
Tsp45I GTSAC 7 cut(s) 1552, 2573, 2753, 2914, 2927, 3013, 3214
TspDTI ATGAA 8 cut(s) 78, 126, 356, 801, 2157, 2267, 2799, 2924
TspGWI ACGGA 2 cut(s) 2161, 3202
TspRI CASTG 9 cut(s) 214, 517, 617, 1660, 2419, 2494, 2997, 3051, 3151
Tth111I GACNNNGTC 1 cut(s) 3011
Van91I CCANNNNNTGG 1 cut(s) 2992
VneI GTGCAC 1 cut(s) 352
VpaK11BI GGWCC 3 cut(s) 1442, 1753, 3210
XagI CCTNNNNNAGG 2 cut(s) 1573, 3273
XapI RAATTY 8 cut(s) 133, 269, 574, 1106, 1167, 1881, 1953, 2065
XbaI TCTAGA 2 cut(s) 343, 2662
XceI RCATGY 3 cut(s) 1216, 1335, 2804
XcmI CCANNNNNNNNNTGG 1 cut(s) 2148
XhoI CTCGAG 1 cut(s) 1157
XmnI GAANNNNTTC 4 cut(s) 290, 684, 1422, 1649
ZrmI AGTACT 1 cut(s) 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.