Rh5AG192100
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011424
Physical Location & Seq
Reverse (-)
22189824 .. 22193836
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG192100.1

Sequence Viewer

Length: 3315 bp
ATGGTTACTTCTTCCCTGAAAATGCTATTACACTTGGGACTCCTCGTGTTATGTTTCAGTTTTGGCTCTACAAACTCATTAAATGAAGAAAGTCTTTTTCTTTTGGAGTTCAAGAGATCCCTATCTGATCCAAGGAACAATCTTGGAAGCTGGAATTCATCACATTTGAGTCCTTGCAATTGGACTGGTGTGAGGTGTCTCAAGTCCAAGGTAACTTCTATAAATCTTACTGGCCTTAATTTGTCTGGTGTTTTGTCTCCAGTCATTTGCAATCTTCCTTACTTGGTTGAATTCAATGTGTCCAAGAACTTCTTTTCTGGGCCATTTCCCAATGATCTTGCTAACTGTCATAATCTAGCGTTTCTAGACCTTTGCACAAATAGGTTTCATGGGGAATTGGTTACCCCATTCACCAAAATGACCAACCTTAGGAAGTTGTATCTCTGTGAGAATTATGTGTTTGGTGAAATGCCTGAGGAGATAGGGAAATTAACTCTAGTTGAAGAGCTTGTGATTTATAGTAATAATCTCACTGGCTCCATTCCTGGTTCAATTAGTAAGTTGAAAAAGTTAAAAGTCCTCAGGGCGGGTCGAAATTCTCTTTCTGGCCCAATACCAACTGTTATCAGTCAGTGTCAAAGCTTAGAAGTTTTGGGGTTATCACAGAATCATTTAGAAGGTTCAATTCCCAGGGAGCTTGAAGAACTTCAAAATCTGACTGATTTGATCCTTTGGCAAAATCATTTAACTGGTTCAGTTCCTGCTGAGATTGGTAATCTAAGTAGGCTAGAGTTGCTTGCCTTGCATGAAAACTCTTTCGGAGGAATGATCCCTAAGGAACTTGGGAAGTTATCACAGCTGAAGAGATTGTATATATACACCAACCAGTTGAATGGAACTATCCCAAGGGAACTAGGGAATTGTACCAATGCGGTTCATATAGATTTTTCTGAAAACCAGTTAAGTGGAGTCATACCACGGGAGTTGGGTTACATTCCTAATCTTTTGTTGCTTCACCTCTTCGAAAATCATCTGGAAGGAAACATTCCGAGGGAGCTTGGCCAGTTGAAGCAGCTGCAGATGTTAGACTTGTCCATAAACAATTTGACTGGCCCAATCCCACTAGAATTTCAGAACCTTCCTTACATGGATGAGTTGCAACTGTTTGATAATCATCTCGAGGGTAAAATTCCTCCTCTCCTTGGAGCTAACAGTAACCTCAGCATTCTTGACATGTCTGCAAATAAGCTTGTGGGTAGCATACCTGCGCATCTTTGCAAGTATGGGAAATTAGCATTTCTAAGCCTTGGGTCAAATAGGTTGTCTGGAAATATTCCTTATGGTATTAAAACATGCAAGTCTTTAGTCCAGCTAATGCTAGGAGACAACCATCTTACAGGAAGTCTCCCTATGGAGTTATATACTCTTTATGCTCTTGAAGTCTTTCAAAACCGATTCTCAGGACCTATACCTCCAGAGGTAGGCAGGTTTAAAAGTTTAGAAAGGCTACTGTTGTCAGATAACTATTTTATTGGGCATATTCCTCTTGAGATTGGCAACCTATCGCAGCTTGTGACCTTCAATGTTTCCTCTAACAGGCTCACGGGGAACATTCCTCATGAGCTAGGAAATTGTACAAAGCTACAGAGGCTTGATCTCAGTAGGAATTACTTCACTGGAGTTCTCCCGGAAGAACTTGGAAAGCTAGTGAATCTGGAACTTTTGAAGCTTTCTGATAACAAGTTGATGGGAGGGATACCGAGTACTCTAGGGGACCTTGCCCGATTGACTGAGTTGGAGATGGGGGGAAATCACTTTTCTGGTAGCGTCCCTTTTCAGCTGGGCCAACTCGGTGCTCTACAGATTGCCCTCAATATTAGCCATAATAATCTTTCGGGTGAAATTCCTGAAAAGTTAGGGGACTTGCAGATGTTGGAATCCCTCTACTTGAATGACAATCAGCTTGTTGGTGAAATTCCTGCCTCAATTGGTGAGTTGCTTAGCCTTCTGGTGTGCAACCTCTCTAACAATAACCTAGTGGGTACTGTGCCAAACACACAAGTATTTCAAAGGATGGATCCTTCAAATTTTGCTGGAAACAATGGATTGTGCAGATCTGGATCATACCATTGTCATCAACCTCCAGTTCAACCTCATACTCCAAAACGGAGCTGGATAAAGGAGGGTTCATCTAAAGAGAAATTAGTAAGCATCATTGCTGCTGTAATTGGAGTAATTTCCTTGATTCTCATAGTTGGTTTCTGTTGGGCAATGAAGCGCTCCAGACCTACTTTTGTTCCAGTTGAAGATCCAATCAAGCCAGACGTGTTGGACAACTATTACTTTCCGAAGGAAGGTTTCAAGTACCAGGATCTTGTTGTAGGCACCAACAATTTTTCAGACAATGCAGTTCTTGGAAGGGGAGCCTGTGGCACTGTGTACAAAGCCGTCATGGCTGATGGAGAGGTGATTGCAGTCAAAAAGCTCAAGGCACAAGGGGAAGGAGTCGGTGTCGATAGCAGCTTCCGCGCTGAGATATCAACCCTCGGGAAGATCAGGCATTGCAATATTGTGAAGCTCTATGGCTTCTGCTGTCACCAAGACTCGAATCTGCTCTTGTATGAGTACATGGAAAATGGCAGTTTAGGAGAACATCTCCATGGAAACGATCAAAGATGTTATCTAGACTGGAATACACGATATAAGATTGCTCGTGGAGCAGCAGAGGGTTTGTGTTATCTCCATTATTACTGCAAGCCCCAAATCGTTCACCGTGACATAAAGTCAAATAACATTTTACTGGATGAAGTTCTTGAAGCACATGTGGGAGATTTCGGCTTGGCAAAATTGATTGAATTGCCATACTCAAAATCCATGTCTGCTGTTGCTGGCTCATATGGCTACATTGCACCAGAGTATGCTTACACAATGAAAGTGACTGAGAAGTGTGACATCTATAGTTTCGGAGTGGTTCTGCTGGAACTAGTAACCGGAAAGACACCCGTCCAACCACTGGAGCTGGGTGGAGACCTAGTCACTCTGGTAAGAAGAGCCATAAATAATTCAGTGGCAACATCCGAATTGTTCGACAAGAGGCTAGATCTGAGTATGAGGAGCACAACTGAAGAGATGACTCTATTTCTCAAGATTGCGTTGTTCTGCACCAGTGTGTCCCCAGTTAAAAGGCCAACAATGAGGGAGGTCATTGCGATGATGATTGATGCTAGGCGGTCCGTGACTGTGAGCAACTGCTCATCACCAACATCTGAGAGTCCTTTGGATAAAGATCCTTCAAGAGATTGTATGGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1104

Amino Acids

122.09

Weight (kDa)

6.22

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 4.1e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 112 - 242 1.5e-12 Leucine-rich repeat region
LRR_8 PF13855 499 - 557 8.4e-06 Leucine rich repeat
LRR_8 PF13855 547 - 606 4.3e-08 Leucine rich repeat
Pkinase PF00069 801 - 1070 1.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 803 - 1070 3.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17230 AT1G17230 AT1G17230
fragaria_vesca FvH4_3g16200 FvH4_3g16200 FvH4_3g16200
malus_domestica MD05G1211500.v1.1
prunus_persica Prupe.4G145700_v2.0.a1
pyrus_communis pycom05g19650
rosa_chinensis RchiOBHm_Chr5g0027271
rosa_laevigata RLG00000033007
rosa_multiflora Rmu_sc0001503.1_g000006
rosa_roxburghii Rroxscaffold_1G00052220 Rroxscaffold_4G00329160
rosa_rugosa Rorug05G0098000
rosa_samantha Rh5AG192100 Rh5BG189800 Rh5CG210200 Rh5DG191800
rosa_wichuraiana Rw5G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1269
Acc36I ACCTGC 2 cut(s) 1273, 1476
AccB1I GGYRCC 1 cut(s) 2384
AccB7I CCANNNNNTGG 1 cut(s) 3013
AccII CGCG 1 cut(s) 2529
AciI CCGC 4 cut(s) 587, 932, 2527, 3229
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
AcuI CTGAAG 2 cut(s) 881, 3144
AfaI GTAC 7 cut(s) 925, 1636, 1765, 2044, 2366, 2441, 2627
AfeI AGCGCT 1 cut(s) 2279
AfiI CCNNNNNNNGG 7 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013
AflIII ACRYGT 3 cut(s) 1233, 2325, 2821
AhdI GACNNNNNGTC 1 cut(s) 2782
AhlI ACTAGT 1 cut(s) 2983
AjiI CACGTC 1 cut(s) 2326
AjnI CCWGG 3 cut(s) 544, 689, 2367
AleI CACNNNNGTG 1 cut(s) 3167
Alw21I GWGCWC 2 cut(s) 1858, 3119
Alw26I GTCTC 5 cut(s) 203, 261, 1377, 1409, 3021
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 2 cut(s) 1178, 2546
Aor51HI AGCGCT 1 cut(s) 2279
AoxI GGCC 7 cut(s) 232, 320, 607, 1060, 1111, 1843, 3185
ApeKI GCWGC 6 cut(s) 1072, 1075, 1567, 2219, 2520, 2720
ApoI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
ArsI GACNNNNNNTTYG 4 cut(s) 1307, 1339, 2861, 2893
Asp700I GAANNNNTTC 3 cut(s) 705, 1443, 1670
AspLEI GCGC 3 cut(s) 1270, 2280, 2531
AspS9I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
AsuC2I CCSGG 1 cut(s) 1688
AsuII TTCGAA 1 cut(s) 1023
AvaI CYCGRG 2 cut(s) 1178, 2546
AvaII GGWCC 3 cut(s) 1463, 1774, 3231
AxyI CCTNAGG 4 cut(s) 428, 474, 581, 834
BalI TGGCCA 1 cut(s) 1062
BamHI GGATCC 1 cut(s) 2077
BanI GGYRCC 1 cut(s) 2384
BauI CACGAG 2 cut(s) 44, 2712
Bbv12I GWGCWC 2 cut(s) 1858, 3119
BbvCI CCTCAGC 1 cut(s) 1220
BbvI GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BccI CCATC 5 cut(s) 1398, 1741, 1795, 2068, 2453
BceAI ACGGC 1 cut(s) 2432
BciT130I CCWGG 3 cut(s) 546, 691, 2369
BciVI GTATCC 1 cut(s) 1749
BcnI CCSGG 1 cut(s) 1688
BcoDI GTCTC 5 cut(s) 203, 261, 1377, 1409, 3021
BcuI ACTAGT 1 cut(s) 2983
BfmI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
BfoI RGCGCY 1 cut(s) 2281
BfuAI ACCTGC 2 cut(s) 1273, 1476
BfuI GTATCC 1 cut(s) 1749
BglI GCCNNNNNGGC 1 cut(s) 2453
BglII AGATCT 2 cut(s) 2114, 3100
BisI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
BlpI GCTNAGC 1 cut(s) 2000
BlsI GCNGC 6 cut(s) 1074, 1077, 1569, 2221, 2522, 2722
BmcAI AGTACT 1 cut(s) 1765
Bme1390I CCNGG 4 cut(s) 546, 691, 1688, 2369
Bme18I GGWCC 3 cut(s) 1463, 1774, 3231
BmeRI GACNNNNNGTC 1 cut(s) 2782
BmeT110I CYCGRG 2 cut(s) 1178, 2546
BmgBI CACGTC 1 cut(s) 2326
BmgT120I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
BmiI GGNNCC 5 cut(s) 538, 1775, 2079, 2386, 2425
BmrFI CCNGG 4 cut(s) 546, 691, 1688, 2369
BmrI ACTGGG 1 cut(s) 3170
BmsI GCATC 3 cut(s) 1279, 2220, 3211
BmuI ACTGGG 1 cut(s) 3170
BoxI GACNNNNGTC 1 cut(s) 3002
BplI GAGNNNNNCTC 2 cut(s) 2640, 2672
BpmI CTGGAG 6 cut(s) 243, 1458, 1698, 2127, 2266, 3035
Bpu10I CCTNAGC 1 cut(s) 1220
Bpu1102I GCTNAGC 1 cut(s) 2000
Bpu14I TTCGAA 1 cut(s) 1023
BpuEI CTTGAG 4 cut(s) 185, 1568, 2471, 3128
BpuMI CCSGG 1 cut(s) 1688
BsaBI GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BsaI GGTCTC 1 cut(s) 3021
BsaWI WCCGGW 1 cut(s) 2990
BsaXI ACNNNNNCTCC 4 cut(s) 2496, 2526, 3018, 3048
Bsc4I CCNNNNNNNGG 7 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013
Bse21I CCTNAGG 4 cut(s) 428, 474, 581, 834
Bse3DI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
Bse8I GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BseBI CCWGG 3 cut(s) 546, 691, 2369
BseGI GGATG 4 cut(s) 1156, 2079, 2809, 3074
BseJI GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BseLI CCNNNNNNNGG 7 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013
BseMI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
BseRI GAGGAG 4 cut(s) 32, 491, 1185, 3127
BseXI GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BseYI CCCAGC 2 cut(s) 1840, 3019
BsgI GTGCAG 2 cut(s) 2131, 3145
Bsh1236I CGCG 1 cut(s) 2529
BshFI GGCC 7 cut(s) 234, 322, 609, 1062, 1113, 1845, 3187
BshNI GGYRCC 1 cut(s) 2384
BsiHKAI GWGCWC 2 cut(s) 1858, 3119
BsiHKCI CYCGRG 2 cut(s) 1178, 2546
BsiSI CCGG 2 cut(s) 1688, 2991
BslFI GGGAC 5 cut(s) 51, 1787, 1814, 1934, 3157
BslI CCNNNNNNNGG 7 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013
BsmAI GTCTC 5 cut(s) 203, 261, 1377, 1409, 3021
BsmFI GGGAC 5 cut(s) 51, 1787, 1814, 1934, 3157
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 7 cut(s) 234, 322, 609, 1062, 1113, 1845, 3187
Bso31I GGTCTC 1 cut(s) 3021
BsoBI CYCGRG 2 cut(s) 1178, 2546
Bsp119I TTCGAA 1 cut(s) 1023
Bsp1286I GDGCHC 2 cut(s) 1858, 3119
Bsp1407I TGTACA 2 cut(s) 1634, 2439
Bsp1720I GCTNAGC 1 cut(s) 2000
Bsp19I CCATGG 1 cut(s) 2659
BspACI CCGC 4 cut(s) 587, 932, 2527, 3229
BspANI GGCC 7 cut(s) 234, 322, 609, 1062, 1113, 1845, 3187
BspFNI CGCG 1 cut(s) 2529
BspHI TCATGA 1 cut(s) 1618
BspLI GGNNCC 5 cut(s) 538, 1775, 2079, 2386, 2425
BspMAI CTGCAG 1 cut(s) 1080
BspMI ACCTGC 2 cut(s) 1273, 1476
BspQI GCTCTTC 2 cut(s) 498, 3043
BspT104I TTCGAA 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 2384
BspTNI GGTCTC 1 cut(s) 3021
BsrDI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
BsrGI TGTACA 2 cut(s) 1634, 2439
BssSI CACGAG 2 cut(s) 44, 2712
BssT1I CCWWGG 6 cut(s) 131, 207, 905, 1201, 1306, 2659
Bst2BI CACGAG 2 cut(s) 44, 2712
Bst2UI CCWGG 3 cut(s) 546, 691, 2369
Bst4CI ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
Bst6I CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
BstAUI TGTACA 2 cut(s) 1634, 2439
BstBI TTCGAA 1 cut(s) 1023
BstC8I GCNNGC 3 cut(s) 798, 2756, 2890
BstDSI CCRYGG 2 cut(s) 977, 2659
BstEII GGTNACC 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 1594
BstF5I GGATG 4 cut(s) 1156, 2079, 2809, 3074
BstFNI CGCG 1 cut(s) 2529
BstH2I RGCGCY 1 cut(s) 2281
BstHHI GCGC 3 cut(s) 1270, 2280, 2531
BstMAI GTCTC 5 cut(s) 203, 261, 1377, 1409, 3021
BstMWI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
BstNI CCWGG 3 cut(s) 546, 691, 2369
BstNSI RCATGY 3 cut(s) 1237, 1356, 2825
BstPAI GACNNNNGTC 1 cut(s) 3002
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 4 cut(s) 544, 689, 1686, 2367
BstSFI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
BstUI CGCG 1 cut(s) 2529
BstV1I GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BstX2I RGATCY 7 cut(s) 116, 2077, 2114, 2308, 2371, 3100, 3286
BstXI CCANNNNNNTGG 2 cut(s) 893, 965
BstYI RGATCY 7 cut(s) 116, 2077, 2114, 2308, 2371, 3100, 3286
Bsu36I CCTNAGG 4 cut(s) 428, 474, 581, 834
BsuI GTATCC 1 cut(s) 1749
BsuRI GGCC 7 cut(s) 234, 322, 609, 1062, 1113, 1845, 3187
BtgI CCRYGG 2 cut(s) 977, 2659
BtgZI GCGATG 1 cut(s) 3224
BtrI CACGTC 1 cut(s) 2326
BtsCI GGATG 4 cut(s) 1156, 2079, 2809, 3074
BtsIMutI CAGTG 7 cut(s) 531, 638, 1674, 2433, 3011, 3072, 3172
BveI ACCTGC 2 cut(s) 1273, 1476
Cac8I GCNNGC 3 cut(s) 798, 2756, 2890
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 1618
CfoI GCGC 3 cut(s) 1270, 2280, 2531
Cfr13I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
CpoI CGGWCCG 1 cut(s) 3231
CseI GACGC 1 cut(s) 1816
Csp6I GTAC 7 cut(s) 924, 1635, 1764, 2043, 2365, 2440, 2626
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CspI CGGWCCG 1 cut(s) 3231
CviQI GTAC 7 cut(s) 924, 1635, 1764, 2043, 2365, 2440, 2626
DraI TTTAAA 1 cut(s) 1492
DriI GACNNNNNGTC 1 cut(s) 2782
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
Eam1105I GACNNNNNGTC 1 cut(s) 2782
EarI CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
Eco130I CCWWGG 6 cut(s) 131, 207, 905, 1201, 1306, 2659
Eco31I GGTCTC 1 cut(s) 3021
Eco32I GATATC 1 cut(s) 2538
Eco47I GGWCC 3 cut(s) 1463, 1774, 3231
Eco47III AGCGCT 1 cut(s) 2279
Eco57I CTGAAG 2 cut(s) 881, 3144
Eco81I CCTNAGG 4 cut(s) 428, 474, 581, 834
Eco88I CYCGRG 2 cut(s) 1178, 2546
Eco91I GGTNACC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 1594
EcoO109I RGGNCCY 2 cut(s) 1463, 1774
EcoO65I GGTNACC 1 cut(s) 400
EcoRI GAATTC 2 cut(s) 154, 290
EcoRII CCWGG 3 cut(s) 544, 689, 2367
EcoRV GATATC 1 cut(s) 2538
EcoT14I CCWWGG 6 cut(s) 131, 207, 905, 1201, 1306, 2659
ErhI CCWWGG 6 cut(s) 131, 207, 905, 1201, 1306, 2659
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 296, 328
FaqI GGGAC 5 cut(s) 51, 1787, 1814, 1934, 3157
FauI CCCGC 1 cut(s) 580
FauNDI CATATG 1 cut(s) 2896
Fnu4HI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
FokI GGATG 4 cut(s) 1163, 2086, 2816, 3061
Fsp4HI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
FspI TGCGCA 1 cut(s) 1269
GlaI GCGC 3 cut(s) 1269, 2279, 2530
GluI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
GsaI CCCAGC 2 cut(s) 1844, 3023
GsuI CTGGAG 6 cut(s) 243, 1458, 1698, 2127, 2266, 3035
HaeII RGCGCY 1 cut(s) 2281
HaeIII GGCC 7 cut(s) 234, 322, 609, 1062, 1113, 1845, 3187
HapII CCGG 2 cut(s) 1688, 2991
HgaI GACGC 1 cut(s) 1816
HhaI GCGC 3 cut(s) 1270, 2280, 2531
Hin6I GCGC 3 cut(s) 1268, 2278, 2529
HinP1I GCGC 3 cut(s) 1268, 2278, 2529
HindIII AAGCTT 3 cut(s) 640, 1247, 1727
HpaII CCGG 2 cut(s) 1688, 2991
Hpy166II GTNNAC 2 cut(s) 2440, 2770
Hpy8I GTNNAC 2 cut(s) 2440, 2770
HpyCH4III ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
HpyCH4IV ACGT 1 cut(s) 2325
HpyF10VI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
HpySE526I ACGT 1 cut(s) 2325
HspAI GCGC 3 cut(s) 1268, 2278, 2529
LguI GCTCTTC 2 cut(s) 498, 3043
Lsp1109I GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
LweI GCATC 3 cut(s) 1279, 2220, 3211
MaeII ACGT 1 cut(s) 2325
MfeI CAATTG 2 cut(s) 178, 1986
MflI RGATCY 7 cut(s) 116, 2077, 2114, 2308, 2371, 3100, 3286
MhlI GDGCHC 2 cut(s) 1858, 3119
MlsI TGGCCA 1 cut(s) 1062
MluNI TGGCCA 1 cut(s) 1062
MlyI GAGTC 7 cut(s) 33, 178, 978, 2514, 2597, 3127, 3280
MmeI TCCRAC 4 cut(s) 1776, 1914, 2310, 3031
Mox20I TGGCCA 1 cut(s) 1062
MroXI GAANNNNTTC 3 cut(s) 705, 1443, 1670
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 9 cut(s) 80, 237, 491, 572, 746, 962, 1347, 1491, 3180
MslI CAYNNNNRTG 4 cut(s) 416, 2658, 3167, 3209
Msp20I TGGCCA 1 cut(s) 1062
MspA1I CMGCKG 3 cut(s) 859, 1075, 1840
MspI CCGG 2 cut(s) 1688, 2991
MspR9I CCNGG 4 cut(s) 546, 691, 1688, 2369
MunI CAATTG 2 cut(s) 178, 1986
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 3 cut(s) 546, 691, 2369
MvnI CGCG 1 cut(s) 2529
MwoI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
NciI CCSGG 1 cut(s) 1688
NcoI CCATGG 1 cut(s) 2659
NdeI CATATG 1 cut(s) 2896
NlaIV GGNNCC 5 cut(s) 538, 1775, 2079, 2386, 2425
NmuCI GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
NsbI TGCGCA 1 cut(s) 1269
NspI RCATGY 3 cut(s) 1237, 1356, 2825
NspV TTCGAA 1 cut(s) 1023
OliI CACNNNNGTG 1 cut(s) 3167
PaeR7I CTCGAG 1 cut(s) 1178
PagI TCATGA 1 cut(s) 1618
PasI CCCWGGG 1 cut(s) 690
PciI ACATGT 2 cut(s) 1233, 2821
PciSI GCTCTTC 2 cut(s) 498, 3043
PctI GAATGC 1 cut(s) 1224
PdmI GAANNNNTTC 3 cut(s) 705, 1443, 1670
PfeI GAWTC 6 cut(s) 667, 1455, 1711, 1937, 2245, 2608
PflFI GACNNNGTC 1 cut(s) 3032
PflMI CCANNNNNTGG 1 cut(s) 3013
PfoI TCCNGGA 1 cut(s) 1686
PkrI GCNGC 6 cut(s) 1074, 1077, 1569, 2221, 2522, 2722
PleI GAGTC 7 cut(s) 33, 177, 977, 2513, 2597, 3127, 3279
PpsI GAGTC 7 cut(s) 33, 177, 977, 2513, 2597, 3127, 3279
PpuMI RGGWCCY 2 cut(s) 1463, 1774
PscI ACATGT 2 cut(s) 1233, 2821
PshAI GACNNNNGTC 1 cut(s) 3002
Psp5II RGGWCCY 2 cut(s) 1463, 1774
Psp6I CCWGG 3 cut(s) 544, 689, 2367
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 2 cut(s) 1840, 3019
PspGI CCWGG 3 cut(s) 544, 689, 2367
PspN4I GGNNCC 5 cut(s) 538, 1775, 2079, 2386, 2425
PspPI GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
PspPPI RGGWCCY 2 cut(s) 1463, 1774
PsrI GAACNNNNNNTAC 2 cut(s) 1127, 1159
PstI CTGCAG 1 cut(s) 1080
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 7 cut(s) 116, 2077, 2114, 2308, 2371, 3100, 3286
PsyI GACNNNGTC 1 cut(s) 3032
PvuII CAGCTG 3 cut(s) 859, 1075, 1840
RsaI GTAC 7 cut(s) 925, 1636, 1765, 2044, 2366, 2441, 2627
RsaNI GTAC 7 cut(s) 924, 1635, 1764, 2043, 2365, 2440, 2626
RseI CAYNNNNRTG 4 cut(s) 416, 2658, 3167, 3209
Rsr2I CGGWCCG 1 cut(s) 3231
RsrII CGGWCCG 1 cut(s) 3231
SapI GCTCTTC 2 cut(s) 498, 3043
SaqAI TTAA 9 cut(s) 80, 237, 491, 572, 746, 962, 1347, 1491, 3180
SatI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
Sau96I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
ScaI AGTACT 1 cut(s) 1765
SchI GAGTC 7 cut(s) 33, 178, 978, 2514, 2597, 3127, 3280
ScrFI CCNGG 4 cut(s) 546, 691, 1688, 2369
SduI GDGCHC 2 cut(s) 1858, 3119
SfaNI GCATC 3 cut(s) 1279, 2220, 3211
SfcI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
Sfr274I CTCGAG 1 cut(s) 1178
SfuI TTCGAA 1 cut(s) 1023
SinI GGWCC 3 cut(s) 1463, 1774, 3231
SlaI CTCGAG 1 cut(s) 1178
SmiMI CAYNNNNRTG 4 cut(s) 416, 2658, 3167, 3209
SmlI CTYRAG 5 cut(s) 200, 1178, 1547, 2486, 3143
SmoI CTYRAG 5 cut(s) 200, 1178, 1547, 2486, 3143
SpeI ACTAGT 1 cut(s) 2983
SsiI CCGC 4 cut(s) 587, 932, 2527, 3229
SspI AATATT 3 cut(s) 1333, 1876, 2569
StyD4I CCNGG 4 cut(s) 544, 689, 1686, 2367
StyI CCWWGG 6 cut(s) 131, 207, 905, 1201, 1306, 2659
TaaI ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
TaiI ACGT 1 cut(s) 2328
TaqI TCGA 6 cut(s) 592, 1023, 1179, 2514, 2606, 3087
TatI WGTACW 4 cut(s) 1634, 1763, 2439, 2625
TfiI GAWTC 6 cut(s) 667, 1455, 1711, 1937, 2245, 2608
Tru1I TTAA 9 cut(s) 80, 237, 491, 572, 746, 962, 1347, 1491, 3180
Tru9I TTAA 9 cut(s) 80, 237, 491, 572, 746, 962, 1347, 1491, 3180
TscAI CASTG 7 cut(s) 538, 638, 1681, 2440, 3018, 3072, 3172
TseFI GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
TseI GCWGC 6 cut(s) 1072, 1075, 1567, 2219, 2520, 2720
Tsp45I GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
TspDTI ATGAA 9 cut(s) 99, 147, 377, 822, 926, 2178, 2288, 2820, 2945
TspGWI ACGGA 2 cut(s) 2182, 3223
TspRI CASTG 7 cut(s) 538, 638, 1681, 2440, 3018, 3072, 3172
Tth111I GACNNNGTC 1 cut(s) 3032
Van91I CCANNNNNTGG 1 cut(s) 3013
VpaK11BI GGWCC 3 cut(s) 1463, 1774, 3231
XagI CCTNNNNNAGG 1 cut(s) 1594
XapI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
XbaI TCTAGA 2 cut(s) 364, 2683
XceI RCATGY 3 cut(s) 1237, 1356, 2825
XcmI CCANNNNNNNNNTGG 1 cut(s) 2169
XhoI CTCGAG 1 cut(s) 1178
XmnI GAANNNNTTC 3 cut(s) 705, 1443, 1670
ZrmI AGTACT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.