Rroxscaffold_1G00052220
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011424
Physical Location & Seq
Forward (+)
72890230 .. 72894069
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052220.1

Sequence Viewer

Length: 2559 bp
ATGGTTTCTTCTTCCCTGAAAATGCTATTACACTTGGGACTCCTCGTGTTATGTTTCAGTTTTGGCTCTACAAACTCATTAGATGAAGAAAGTCTTTTTCTTTTGGAGTTCAAGAGATCCCTATCTGATCCAAGTAACAATCTTGGAAGCTGGAATTCATCACATTTGAGTCCTTGCAATTGGACTGGTGTGAGGTGTCTCAAGTCCAAGGTAACTTCTATAAATCTCACTGGCCTTAATTTGTCTGGTGTTTTGTCTCCAGTCATTTGCAATCTTCCTTACTTGGTTGAATTCAATGTGTCCAAGAACTTCTTCTCTGGGCCATTTCCCATTGATCTTGCTAACTGTCATAATCTAGCGTTTCTAGACCTTTGCACAAATAGGGCGGGTCGAAATTCTCTTTCTGGCCCAATACCAACTGTTATCAGTCAGTGTCAGAGCTTAGAAGTTTTGGGGTTATCACAGAATCATTTAGAAGGTTCAATTCCCAGGGAGCTTGAAGAACTTCAAAATCTGACTGATTTGATCCTTTGGCAAAATCATTTAACTGGTTCAGTTCCTGCTGAGATTGGTAATCTAAGTAGGCTAGAGTTGCTTGCCTTGCATGAAAACTCTTTCGGAGGAATGATCCCTAAGGAACTTGGGAAGTTATCACAGCTGAAGAGATTGTATATATATACCAACCAGTTGAATGGAACTATCCCAAGTGAACTAGGGAATTGTACCAATACGGTTCATATAGATTTTTCTGAAAACCAGTTAAGTGGAGTCATACCACGGGAGTTGGGTTACATTCCTAATCTTGTGTTGCTTCACCTCTTCGAAAATCATCTGGAAGGAAACATTCCGAGGGAGCTTGGCCAGTTGAAGCAGCTGCAGGGGTTAGACTTGTCCATAAACAATTTGACTGGCCCAATCCCACTAGAATTTCAGAACCTTACTTACATGGATGAGTTGCAACTGTTTGATAATCATCTCGAGGGTAAAATTCCTCCTCTCCTTGGAGCTAACAGTTACCTCAGCATTCTTGACATGTCTGCAAATAAGCTTGTAGGTAGCATCCCTGCGCATCTTTGCGAGTATGGGAAATTAGCATTTCTAAGCCTTGGGTCAAATAGGTTGTCTGGAAATATTCCTTATGGTATTAAAACATGCAAGTCTCTAGTCCAGCTAATGCTAGGAGACAACCATCTTACAGGAAGTCTCCCTATGGAGTTATATACTCTTTATGCTCTTGAAGTCTTTCAAAACCGATTCTCAGGACCTATACCTCCAGAGGTAGGCAGGTTTAAAAGTTTAGAAAGGCTACTGTTGTCAGATAACTATTTTATTGGGCATATTCCTCTTGAGATTGGCAACCTATCGCAGCTTGTGACCTTCAATGTTTCCTCTAACAGGCTCACGGGGAACATTCCTCATGAGCTGGGAAATTGTACAAAGCTACAGAGGCTTGATCTCAGTAGGAATTACTTCACTGGAGTTCTCCCGGAAGAACTTGGAAAGCTAGTGAATCTGGAACTTTTGAAGCTTTCTGATAACAAGTTGATGGGAGGGATACCGAGTACTCTAGGGGACCTTGCCCGATTGACTGAGTTGGAGATGGGGGAAATCACTTTTCTGGTAGCATCCCTTTTCAGCTGGGCCAACTCGATGTTGGAATCCCTCTACTTGAATGACAATCAGCTTGTTGGTGAAATTCCTGCCTCAATTGGTGAGTTGCTTAGCCTTCTGGTGTGCAACCTCTCTAACAATAACCTAGTGGGAACTGTGCCAAACACACAAGTATTTCAAAGGATGGATTCTTCAAATTTTGCTGGAAACAATGGATTGTGCAGATCTGGATCATACCATTGTCATCAACCTTCAGTTGAATCTCATACTCCAAAACGGAGCTGGATAAAGGAGGGTTCATCGAAAGAGAAATTAGTAAGCATCATTGCTGCTGTAATTGGAGTAATTTCCTTGATTCTCATAGTTGGTTTCTGTTGGGCAATGAAGCGCTCCAGACCTACTTCTGTTCCACATGAAGATCCAATTAAGCCAGATGTGTTGGACAACTATTACTTTCCGAAGGAAGGCTTCAATTTAGGAGAACATCTCCATGGAAACGATCAAAGGTGTTATCTAGACTGGAATACACGATATAAGATTGCTCTTGGAGCAGCAGAGGAGTATGCTTACACAATGAAAGTGACTGAGAAGTGTGACATCTATAGTTTCGGAGTGGTTCTGCTGGAACTGGTAACCGGAAAGACACCAGTCCAACCACTGGAGCTGGGTGGAGACCTAGTGAATCTGGTAAGAAGAGCCATAAATAATTCAGTGGCAACATCCGAATTGTTCGACAAGAGGCTAGATCTGAGTATGAGGAGCACAACTGAAGAGATGACTCTATTTCTCAAGATTGCGTTGTTCTGCACCAGTGTGTCCCCAGTTAAAAGGCCAACAATGAGGGAGGTCATTGCGATGATGATTGATGCTAGGCGGTCTGTGACTGTGAGCAACTGCTCATCACCAACATCTGAGAGTCCTTTGGATAAAGATCCTTCAAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

852

Amino Acids

94.2

Weight (kDa)

5.68

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 7.6e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 142 - 248 1.4e-08 Leucine-rich repeat region
LRR_14 PF23598 423 - 573 8.1e-09 Leucine-rich repeat region
LRR_8 PF13855 432 - 490 6e-06 Leucine rich repeat
LRR_8 PF13855 480 - 534 3.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17230 AT1G17230 AT1G17230
fragaria_vesca FvH4_3g16200 FvH4_3g16200 FvH4_3g16200
malus_domestica MD05G1211500.v1.1
prunus_persica Prupe.4G145700_v2.0.a1
pyrus_communis pycom05g19650
rosa_chinensis RchiOBHm_Chr5g0027271
rosa_laevigata RLG00000033007
rosa_multiflora Rmu_sc0001503.1_g000006
rosa_roxburghii Rroxscaffold_1G00052220 Rroxscaffold_4G00329160
rosa_rugosa Rorug05G0098000
rosa_samantha Rh5AG192100 Rh5BG189800 Rh5CG210200 Rh5DG191800
rosa_wichuraiana Rw5G017490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1068
Acc36I ACCTGC 1 cut(s) 1275
AccB7I CCANNNNNTGG 1 cut(s) 2269
AciI CCGC 2 cut(s) 386, 2485
AclWI GGATC 7 cut(s) 111, 122, 520, 622, 1849, 2024, 2537
AcoI YGGCCR 1 cut(s) 859
AcsI RAATTY 7 cut(s) 154, 290, 394, 926, 987, 1695, 1807
AcuI CTGAAG 3 cut(s) 680, 1848, 2400
AfaI GTAC 3 cut(s) 724, 1435, 1564
AfeI AGCGCT 1 cut(s) 2000
AfiI CCNNNNNNNGG 5 cut(s) 1001, 1280, 1395, 2075, 2269
AflIII ACRYGT 1 cut(s) 1032
AjnI CCWGG 1 cut(s) 488
AjuI GAANNNNNNNTTGG 2 cut(s) 296, 328
AleI CACNNNNGTG 1 cut(s) 2423
Alw21I GWGCWC 1 cut(s) 2375
Alw26I GTCTC 6 cut(s) 203, 261, 1164, 1176, 1208, 2277
AlwI GGATC 7 cut(s) 111, 122, 520, 622, 1849, 2024, 2537
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 977
Aor51HI AGCGCT 1 cut(s) 2000
AoxI GGCC 7 cut(s) 232, 320, 406, 859, 910, 1641, 2441
ApeKI GCWGC 5 cut(s) 871, 874, 1366, 1940, 2162
ApoI RAATTY 7 cut(s) 154, 290, 394, 926, 987, 1695, 1807
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
Asp700I GAANNNNTTC 4 cut(s) 311, 504, 1242, 1469
AspLEI GCGC 2 cut(s) 1069, 2001
AspS9I GGNCC 6 cut(s) 320, 407, 911, 1262, 1573, 1641
AsuC2I CCSGG 1 cut(s) 1487
AsuHPI GGTGA 4 cut(s) 806, 1703, 1724, 2505
AsuII TTCGAA 1 cut(s) 822
AvaI CYCGRG 1 cut(s) 977
AvaII GGWCC 2 cut(s) 1262, 1573
AxyI CCTNAGG 1 cut(s) 633
BalI TGGCCA 1 cut(s) 861
BauI CACGAG 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 2375
BbvCI CCTCAGC 1 cut(s) 1019
BbvI GCAGC 5 cut(s) 861, 883, 1378, 1927, 2174
BccI CCATC 4 cut(s) 1197, 1540, 1594, 1789
BciT130I CCWGG 1 cut(s) 490
BciVI GTATCC 1 cut(s) 1548
BcnI CCSGG 1 cut(s) 1487
BcoDI GTCTC 6 cut(s) 203, 261, 1164, 1176, 1208, 2277
BfmI CTRYAG 3 cut(s) 875, 1442, 2212
BfoI RGCGCY 1 cut(s) 2002
BfuAI ACCTGC 1 cut(s) 1275
BfuI GTATCC 1 cut(s) 1548
BglII AGATCT 2 cut(s) 1835, 2356
BisI GCNGC 5 cut(s) 872, 875, 1367, 1941, 2163
BlpI GCTNAGC 1 cut(s) 1721
BlsI GCNGC 5 cut(s) 873, 876, 1368, 1942, 2164
BmcAI AGTACT 1 cut(s) 1564
Bme1390I CCNGG 2 cut(s) 490, 1487
Bme18I GGWCC 2 cut(s) 1262, 1573
BmeT110I CYCGRG 1 cut(s) 977
BmgT120I GGNCC 6 cut(s) 320, 407, 911, 1262, 1573, 1641
BmiI GGNNCC 1 cut(s) 1574
BmrFI CCNGG 2 cut(s) 490, 1487
BmrI ACTGGG 1 cut(s) 2426
BmsI GCATC 5 cut(s) 1068, 1078, 1634, 1941, 2467
BmuI ACTGGG 1 cut(s) 2426
BoxI GACNNNNGTC 1 cut(s) 2258
BplI GAGNNNNNCTC 2 cut(s) 2082, 2114
BpmI CTGGAG 5 cut(s) 243, 1257, 1497, 1987, 2291
Bpu10I CCTNAGC 1 cut(s) 1019
Bpu1102I GCTNAGC 1 cut(s) 1721
Bpu14I TTCGAA 1 cut(s) 822
BpuEI CTTGAG 3 cut(s) 185, 1367, 2384
BpuMI CCSGG 1 cut(s) 1487
BsaBI GATNNNNATC 4 cut(s) 121, 972, 1840, 2541
BsaI GGTCTC 1 cut(s) 2277
BsaJI CCNNGG 8 cut(s) 207, 488, 489, 776, 848, 1000, 1105, 2101
BsaWI WCCGGW 1 cut(s) 2246
BsaXI ACNNNNNCTCC 2 cut(s) 2274, 2304
Bsc4I CCNNNNNNNGG 5 cut(s) 1001, 1280, 1395, 2075, 2269
Bse21I CCTNAGG 1 cut(s) 633
Bse3DI GCAATG 3 cut(s) 1935, 1998, 2460
Bse8I GATNNNNATC 4 cut(s) 121, 972, 1840, 2541
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 8 cut(s) 207, 488, 489, 776, 848, 1000, 1105, 2101
BseGI GGATG 5 cut(s) 955, 1059, 1625, 1800, 2330
BseJI GATNNNNATC 4 cut(s) 121, 972, 1840, 2541
BseLI CCNNNNNNNGG 5 cut(s) 1001, 1280, 1395, 2075, 2269
BseMI GCAATG 3 cut(s) 1935, 1998, 2460
BseMII CTCAG 8 cut(s) 555, 1033, 1272, 1471, 1581, 2187, 2351, 2514
BseRI GAGGAG 4 cut(s) 32, 984, 2183, 2383
BseXI GCAGC 5 cut(s) 861, 883, 1378, 1927, 2174
BseYI CCCAGC 3 cut(s) 1423, 1638, 2275
BsgI GTGCAG 2 cut(s) 1852, 2401
BshFI GGCC 7 cut(s) 234, 322, 408, 861, 912, 1643, 2443
BsiHKAI GWGCWC 1 cut(s) 2375
BsiHKCI CYCGRG 1 cut(s) 977
BsiSI CCGG 2 cut(s) 1487, 2247
BslFI GGGAC 3 cut(s) 51, 1586, 2413
BslI CCNNNNNNNGG 5 cut(s) 1001, 1280, 1395, 2075, 2269
BsmAI GTCTC 6 cut(s) 203, 261, 1164, 1176, 1208, 2277
BsmFI GGGAC 3 cut(s) 51, 1586, 2413
BsmI GAATGC 1 cut(s) 1023
BsnI GGCC 7 cut(s) 234, 322, 408, 861, 912, 1643, 2443
Bso31I GGTCTC 1 cut(s) 2277
BsoBI CYCGRG 1 cut(s) 977
Bsp119I TTCGAA 1 cut(s) 822
Bsp1286I GDGCHC 1 cut(s) 2375
Bsp1407I TGTACA 1 cut(s) 1433
Bsp1720I GCTNAGC 1 cut(s) 1721
Bsp19I CCATGG 1 cut(s) 2101
BspACI CCGC 2 cut(s) 386, 2485
BspANI GGCC 7 cut(s) 234, 322, 408, 861, 912, 1643, 2443
BspCNI CTCAG 8 cut(s) 556, 1032, 1271, 1470, 1582, 2188, 2352, 2515
BspHI TCATGA 1 cut(s) 1417
BspLI GGNNCC 1 cut(s) 1574
BspMAI CTGCAG 1 cut(s) 879
BspMI ACCTGC 1 cut(s) 1275
BspPI GGATC 7 cut(s) 111, 122, 520, 622, 1849, 2024, 2537
BspQI GCTCTTC 1 cut(s) 2299
BspT104I TTCGAA 1 cut(s) 822
BspTNI GGTCTC 1 cut(s) 2277
BsrDI GCAATG 3 cut(s) 1935, 1998, 2460
BsrGI TGTACA 1 cut(s) 1433
BssECI CCNNGG 8 cut(s) 207, 488, 489, 776, 848, 1000, 1105, 2101
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 4 cut(s) 207, 1000, 1105, 2101
Bst2BI CACGAG 1 cut(s) 44
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 8 cut(s) 347, 421, 733, 963, 1013, 1311, 1768, 2497
Bst6I CTCTTC 4 cut(s) 656, 824, 2299, 2376
BstAUI TGTACA 1 cut(s) 1433
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 1 cut(s) 597
BstDSI CCRYGG 2 cut(s) 776, 2101
BstEII GGTNACC 1 cut(s) 2242
BstENI CCTNNNNNAGG 1 cut(s) 1393
BstF5I GGATG 5 cut(s) 955, 1059, 1625, 1800, 2330
BstH2I RGCGCY 1 cut(s) 2002
BstHHI GCGC 2 cut(s) 1069, 2001
BstMAI GTCTC 6 cut(s) 203, 261, 1164, 1176, 1208, 2277
BstMWI GCNNNNNNNGC 4 cut(s) 592, 601, 1447, 2159
BstNI CCWGG 1 cut(s) 490
BstNSI RCATGY 2 cut(s) 1036, 1155
BstPAI GACNNNNGTC 1 cut(s) 2258
BstPI GGTNACC 1 cut(s) 2242
BstSCI CCNGG 2 cut(s) 488, 1485
BstSFI CTRYAG 3 cut(s) 875, 1442, 2212
BstV1I GCAGC 5 cut(s) 861, 883, 1378, 1927, 2174
BstX2I RGATCY 5 cut(s) 116, 1835, 2029, 2356, 2542
BstXI CCANNNNNNTGG 2 cut(s) 692, 764
BstYI RGATCY 5 cut(s) 116, 1835, 2029, 2356, 2542
Bsu36I CCTNAGG 1 cut(s) 633
BsuI GTATCC 1 cut(s) 1548
BsuRI GGCC 7 cut(s) 234, 322, 408, 861, 912, 1643, 2443
BtgI CCRYGG 2 cut(s) 776, 2101
BtgZI GCGATG 1 cut(s) 2480
BtsCI GGATG 5 cut(s) 955, 1059, 1625, 1800, 2330
BtsIMutI CAGTG 6 cut(s) 228, 437, 1473, 2267, 2328, 2428
BveI ACCTGC 1 cut(s) 1275
Cac8I GCNNGC 1 cut(s) 597
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 1417
CfoI GCGC 2 cut(s) 1069, 2001
Cfr13I GGNCC 6 cut(s) 320, 407, 911, 1262, 1573, 1641
Csp6I GTAC 3 cut(s) 723, 1434, 1563
CspCI CAANNNNNGTGG 2 cut(s) 765, 800
CviAII CATG 7 cut(s) 605, 946, 1033, 1152, 1418, 2024, 2102
CviQI GTAC 3 cut(s) 723, 1434, 1563
DraI TTTAAA 1 cut(s) 1291
EaeI YGGCCR 1 cut(s) 859
Eam1104I CTCTTC 4 cut(s) 656, 824, 2299, 2376
EarI CTCTTC 4 cut(s) 656, 824, 2299, 2376
Eco130I CCWWGG 4 cut(s) 207, 1000, 1105, 2101
Eco31I GGTCTC 1 cut(s) 2277
Eco47I GGWCC 2 cut(s) 1262, 1573
Eco47III AGCGCT 1 cut(s) 2000
Eco57I CTGAAG 3 cut(s) 680, 1848, 2400
Eco81I CCTNAGG 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 977
Eco91I GGTNACC 1 cut(s) 2242
EcoNI CCTNNNNNAGG 1 cut(s) 1393
EcoO109I RGGNCCY 2 cut(s) 1262, 1573
EcoO65I GGTNACC 1 cut(s) 2242
EcoRI GAATTC 2 cut(s) 154, 290
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 4 cut(s) 207, 1000, 1105, 2101
ErhI CCWWGG 4 cut(s) 207, 1000, 1105, 2101
FaeI CATG 7 cut(s) 608, 949, 1036, 1155, 1421, 2027, 2105
FalI AAGNNNNNCTT 6 cut(s) 78, 110, 296, 328, 2063, 2095
FaqI GGGAC 3 cut(s) 51, 1586, 2413
FatI CATG 7 cut(s) 604, 945, 1032, 1151, 1417, 2023, 2101
FauI CCCGC 1 cut(s) 379
Fnu4HI GCNGC 5 cut(s) 872, 875, 1367, 1941, 2163
FokI GGATG 5 cut(s) 962, 1046, 1612, 1807, 2317
Fsp4HI GCNGC 5 cut(s) 872, 875, 1367, 1941, 2163
FspI TGCGCA 1 cut(s) 1068
GlaI GCGC 2 cut(s) 1068, 2000
GluI GCNGC 5 cut(s) 872, 875, 1367, 1941, 2163
GsaI CCCAGC 3 cut(s) 1427, 1642, 2279
GsuI CTGGAG 5 cut(s) 243, 1257, 1497, 1987, 2291
HaeII RGCGCY 1 cut(s) 2002
HaeIII GGCC 7 cut(s) 234, 322, 408, 861, 912, 1643, 2443
HapII CCGG 2 cut(s) 1487, 2247
HhaI GCGC 2 cut(s) 1069, 2001
Hin1II CATG 7 cut(s) 608, 949, 1036, 1155, 1421, 2027, 2105
Hin6I GCGC 2 cut(s) 1067, 1999
HinP1I GCGC 2 cut(s) 1067, 1999
HindIII AAGCTT 2 cut(s) 1046, 1526
HpaII CCGG 2 cut(s) 1487, 2247
HphI GGTGA 4 cut(s) 806, 1703, 1724, 2505
Hpy166II GTNNAC 1 cut(s) 710
Hpy8I GTNNAC 1 cut(s) 710
HpyAV CCTTC 8 cut(s) 470, 830, 1387, 1736, 1872, 2065, 2069, 2556
HpyCH4III ACNGT 8 cut(s) 347, 421, 733, 963, 1013, 1311, 1768, 2497
HpyF10VI GCNNNNNNNGC 4 cut(s) 592, 601, 1447, 2159
Hsp92II CATG 7 cut(s) 608, 949, 1036, 1155, 1421, 2027, 2105
HspAI GCGC 2 cut(s) 1067, 1999
LguI GCTCTTC 1 cut(s) 2299
LmnI GCTCC 8 cut(s) 493, 853, 1004, 1890, 2006, 2159, 2272, 2370
Lsp1109I GCAGC 5 cut(s) 861, 883, 1378, 1927, 2174
LweI GCATC 5 cut(s) 1068, 1078, 1634, 1941, 2467
MaeIII GTNAC 9 cut(s) 134, 211, 788, 1013, 1372, 2191, 2204, 2242, 2491
MfeI CAATTG 2 cut(s) 178, 1707
MflI RGATCY 5 cut(s) 116, 1835, 2029, 2356, 2542
MhlI GDGCHC 1 cut(s) 2375
MlsI TGGCCA 1 cut(s) 861
MluNI TGGCCA 1 cut(s) 861
MlyI GAGTC 5 cut(s) 33, 178, 777, 2383, 2536
MmeI TCCRAC 4 cut(s) 1575, 1635, 2031, 2287
Mox20I TGGCCA 1 cut(s) 861
MroXI GAANNNNTTC 4 cut(s) 311, 504, 1242, 1469
MscI TGGCCA 1 cut(s) 861
MseI TTAA 7 cut(s) 237, 545, 761, 1146, 1290, 2037, 2436
MslI CAYNNNNRTG 3 cut(s) 2100, 2423, 2465
Msp20I TGGCCA 1 cut(s) 861
MspA1I CMGCKG 3 cut(s) 658, 874, 1638
MspI CCGG 2 cut(s) 1487, 2247
MspR9I CCNGG 2 cut(s) 490, 1487
MunI CAATTG 2 cut(s) 178, 1707
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 490
MwoI GCNNNNNNNGC 4 cut(s) 592, 601, 1447, 2159
NciI CCSGG 1 cut(s) 1487
NcoI CCATGG 1 cut(s) 2101
NlaIII CATG 7 cut(s) 608, 949, 1036, 1155, 1421, 2027, 2105
NlaIV GGNNCC 1 cut(s) 1574
NmuCI GTSAC 4 cut(s) 1372, 2191, 2204, 2491
NsbI TGCGCA 1 cut(s) 1068
NspI RCATGY 2 cut(s) 1036, 1155
NspV TTCGAA 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 2423
PaeR7I CTCGAG 1 cut(s) 977
PagI TCATGA 1 cut(s) 1417
PasI CCCWGGG 1 cut(s) 489
PciI ACATGT 1 cut(s) 1032
PciSI GCTCTTC 1 cut(s) 2299
PctI GAATGC 1 cut(s) 1023
PdmI GAANNNNTTC 4 cut(s) 311, 504, 1242, 1469
PfeI GAWTC 8 cut(s) 466, 1254, 1510, 1658, 1799, 1871, 1966, 2293
PflMI CCANNNNNTGG 1 cut(s) 2269
PfoI TCCNGGA 1 cut(s) 1485
PkrI GCNGC 5 cut(s) 873, 876, 1368, 1942, 2164
PleI GAGTC 5 cut(s) 33, 177, 776, 2383, 2535
PpsI GAGTC 5 cut(s) 33, 177, 776, 2383, 2535
PpuMI RGGWCCY 2 cut(s) 1262, 1573
PscI ACATGT 1 cut(s) 1032
PshAI GACNNNNGTC 1 cut(s) 2258
Psp5II RGGWCCY 2 cut(s) 1262, 1573
Psp6I CCWGG 1 cut(s) 488
PspEI GGTNACC 1 cut(s) 2242
PspFI CCCAGC 3 cut(s) 1423, 1638, 2275
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 1574
PspPI GGNCC 6 cut(s) 320, 407, 911, 1262, 1573, 1641
PspPPI RGGWCCY 2 cut(s) 1262, 1573
PsrI GAACNNNNNNTAC 2 cut(s) 926, 958
PstI CTGCAG 1 cut(s) 879
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 5 cut(s) 116, 1835, 2029, 2356, 2542
PvuII CAGCTG 3 cut(s) 658, 874, 1638
RsaI GTAC 3 cut(s) 724, 1435, 1564
RsaNI GTAC 3 cut(s) 723, 1434, 1563
RseI CAYNNNNRTG 3 cut(s) 2100, 2423, 2465
SapI GCTCTTC 1 cut(s) 2299
SaqAI TTAA 7 cut(s) 237, 545, 761, 1146, 1290, 2037, 2436
SatI GCNGC 5 cut(s) 872, 875, 1367, 1941, 2163
Sau96I GGNCC 6 cut(s) 320, 407, 911, 1262, 1573, 1641
ScaI AGTACT 1 cut(s) 1564
SchI GAGTC 5 cut(s) 33, 178, 777, 2383, 2536
ScrFI CCNGG 2 cut(s) 490, 1487
SduI GDGCHC 1 cut(s) 2375
SfaNI GCATC 5 cut(s) 1068, 1078, 1634, 1941, 2467
SfcI CTRYAG 3 cut(s) 875, 1442, 2212
Sfr274I CTCGAG 1 cut(s) 977
SfuI TTCGAA 1 cut(s) 822
SinI GGWCC 2 cut(s) 1262, 1573
SlaI CTCGAG 1 cut(s) 977
SmiMI CAYNNNNRTG 3 cut(s) 2100, 2423, 2465
SmlI CTYRAG 4 cut(s) 200, 977, 1346, 2399
SmoI CTYRAG 4 cut(s) 200, 977, 1346, 2399
SsiI CCGC 2 cut(s) 386, 2485
SspI AATATT 1 cut(s) 1132
StyD4I CCNGG 2 cut(s) 488, 1485
StyI CCWWGG 4 cut(s) 207, 1000, 1105, 2101
TaaI ACNGT 8 cut(s) 347, 421, 733, 963, 1013, 1311, 1768, 2497
TaqI TCGA 6 cut(s) 391, 822, 978, 1649, 1913, 2343
TatI WGTACW 2 cut(s) 1433, 1562
TfiI GAWTC 8 cut(s) 466, 1254, 1510, 1658, 1799, 1871, 1966, 2293
Tru1I TTAA 7 cut(s) 237, 545, 761, 1146, 1290, 2037, 2436
Tru9I TTAA 7 cut(s) 237, 545, 761, 1146, 1290, 2037, 2436
TscAI CASTG 6 cut(s) 235, 437, 1480, 2274, 2328, 2428
TseFI GTSAC 4 cut(s) 1372, 2191, 2204, 2491
TseI GCWGC 5 cut(s) 871, 874, 1366, 1940, 2162
Tsp45I GTSAC 4 cut(s) 1372, 2191, 2204, 2491
TspDTI ATGAA 8 cut(s) 99, 147, 621, 725, 1899, 2009, 2040, 2201
TspGWI ACGGA 1 cut(s) 1903
TspRI CASTG 6 cut(s) 235, 437, 1480, 2274, 2328, 2428
Van91I CCANNNNNTGG 1 cut(s) 2269
VpaK11BI GGWCC 2 cut(s) 1262, 1573
XagI CCTNNNNNAGG 1 cut(s) 1393
XapI RAATTY 7 cut(s) 154, 290, 394, 926, 987, 1695, 1807
XbaI TCTAGA 2 cut(s) 364, 2125
XceI RCATGY 2 cut(s) 1036, 1155
XcmI CCANNNNNNNNNTGG 2 cut(s) 1651, 1890
XhoI CTCGAG 1 cut(s) 977
XmnI GAANNNNTTC 4 cut(s) 311, 504, 1242, 1469
ZrmI AGTACT 1 cut(s) 1564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.