MD05G1215200.v1.1

Photosystem II 10 kDa polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016135
Physical Location & Seq
Forward (+)
34616426 .. 34618498
2073 bp
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UTR
Exon/CDS
Intron
MD05G1215200.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGCAGCCTCAGTCATGACTTCTTCAGTGAGCCTAAAACCATCTCCTCTCACTGTTGACAAGTCAGCAGTGAGAGGCCTTCCCTCTCTTTCCAGGTCTTCTGCTTCATTCAAGGTGCAAGCAAGTGGCGGCAAGAAGATCAAGACTGCCACCCCCTATGGAATTAATGGCGGCATGGACTTGAGGGAGGGTGTCGATGCCTCCGGGAGGAAGGGTAAGGGAAAGGGTGTCTACCAGTTTGTAGACAAGTACGGTGCTAATGTTGATGGATACAGCCCCATCTACAACAAAGATGACTGGTCTCCAAGTGGTGATGTCTATGCTGGGGGTGTTACTGGCTTGTCCATCTGGGCAGTTACCCTAGTTGGCATTCTTGCAGGGGGTGCTCTTCTTGTCTACAACACAAGTGCTTTGTCACAGTAA

Protein Analysis

141

Amino Acids

14.35

Weight (kDa)

9.58

Isoelectric Point (pI)

18.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbR PF04725 42 - 139 8.7e-51 Photosystem II 10 kDa polypeptide PsbR
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79040
fragaria_vesca FvH4_3g16050
malus_domestica MD05G1215200.v1.1 MD10G1198300.v1.1
prunus_persica Prupe.4G143700_v2.0.a1
pyrus_communis pycom05g19820 pycom10g17100
rosa_chinensis RchiOBHm_Chr5g0027021
rosa_laevigata RLG00000032979 RLG00000032983
rosa_roxburghii Rroxscaffold_1G00052470
rosa_rugosa Rorug05G0096600 Rorug05G0096700
rosa_samantha Rh5AG190200
rosa_wichuraiana Rw5G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 231, 243, 396
AciI CCGC 2 cut(s) 129, 171
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 1 cut(s) 92
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 306
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 165
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 49, 260, 287, 353
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 1 cut(s) 362
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 3 cut(s) 6, 130, 172
BlsI GCNGC 3 cut(s) 7, 131, 173
Bme1390I CCNGG 2 cut(s) 94, 205
BmrFI CCNGG 2 cut(s) 94, 205
BmsI GCATC 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 202
BpuMI CCSGG 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 3 cut(s) 235, 302, 340
BseBI CCWGG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 3 cut(s) 235, 302, 340
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 1 cut(s) 78
Bso31I GGTCTC 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 2 cut(s) 129, 171
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 15
BspQI GCTCTTC 1 cut(s) 393
BspTNI GGTCTC 1 cut(s) 306
BsrI ACTGG 3 cut(s) 235, 302, 340
BssMI GATC 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 3 cut(s) 55, 254, 420
Bst6I CTCTTC 1 cut(s) 393
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 10
BstENI CCTNNNNNAGG 1 cut(s) 205
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 92, 203
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 90
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 3 cut(s) 33, 51, 75
Cac8I GCNNGC 1 cut(s) 120
CciI TCATGA 1 cut(s) 15
Csp6I GTAC 1 cut(s) 250
CviAII CATG 2 cut(s) 16, 175
CviJI RGCY 5 cut(s) 8, 33, 78, 276, 339
CviKI_1 RGCY 5 cut(s) 8, 33, 78, 276, 339
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 10
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco147I AGGCCT 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 92
FaeI CATG 2 cut(s) 19, 178
FaiI YATR 4 cut(s) 17, 159, 176, 321
FatI CATG 2 cut(s) 15, 174
FblI GTMKAC 3 cut(s) 231, 243, 396
Fnu4HI GCNGC 3 cut(s) 6, 130, 172
Fsp4HI GCNGC 3 cut(s) 6, 130, 172
FspBI CTAG 1 cut(s) 362
GluI GCNGC 3 cut(s) 6, 130, 172
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 204
Hin1II CATG 2 cut(s) 19, 178
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 4 cut(s) 58, 232, 244, 397
Hpy188III TCNNGA 2 cut(s) 16, 142
Hpy8I GTNNAC 4 cut(s) 58, 232, 244, 397
HpyAV CCTTC 2 cut(s) 89, 205
HpyCH4III ACNGT 3 cut(s) 55, 254, 420
HpyCH4V TGCA 2 cut(s) 118, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 1 cut(s) 10
Hsp92II CATG 2 cut(s) 19, 178
Kzo9I GATC 1 cut(s) 138
LguI GCTCTTC 1 cut(s) 393
LpnPI CCDG 9 cut(s) 79, 106, 217, 248, 283, 309, 321, 334, 363
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 187
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 3 cut(s) 331, 355, 414
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 4 cut(s) 15, 90, 148, 380
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 1 cut(s) 162
MnlI CCTC 8 cut(s) 19, 57, 68, 94, 177, 181, 201, 211
MseI TTAA 1 cut(s) 165
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 2 cut(s) 94, 205
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 383
NciI CCSGG 1 cut(s) 205
NdeII GATC 1 cut(s) 138
NlaIII CATG 2 cut(s) 19, 178
NmuCI GTSAC 1 cut(s) 414
PagI TCATGA 1 cut(s) 15
PceI AGGCCT 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 393
PctI GAATGC 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 3 cut(s) 7, 131, 173
PshBI ATTAAT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 92
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 3 cut(s) 6, 130, 172
Sau3AI GATC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 94, 205
SduI GDGCHC 1 cut(s) 388
SetI ASST 2 cut(s) 98, 117
SfaNI GCATC 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 1 cut(s) 162
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 2 cut(s) 129, 171
SspMI CTAG 1 cut(s) 362
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 92, 203
TaaI ACNGT 3 cut(s) 55, 254, 420
TaqI TCGA 1 cut(s) 195
TasI AATT 1 cut(s) 162
TauI GCSGC 2 cut(s) 132, 174
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 3 cut(s) 33, 58, 75
TseFI GTSAC 1 cut(s) 414
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 414
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 3 cut(s) 33, 58, 75
VspI ATTAAT 1 cut(s) 165
XagI CCTNNNNNAGG 1 cut(s) 205
XmiI GTMKAC 3 cut(s) 231, 243, 396
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.