Rorug05G0096700

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008387
Physical Location & Seq
Forward (+)
8563255 .. 8568214
4960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0096700.1

Sequence Viewer

Length: 1662 bp
ATGGAAAACAGCAACAAGGGTAACACAGGTGACAACATTTCTCATCCCGGTAGCAATAAGCCGAAGAAACCAGATGGCCATAAGAATGGTTATAGTAAAGATGTCATGCCTGGAAGTGAACTTTGGACAGATGGGCTTATCTGTGCTTTTGAGTTCATTCGAGGCCGAAAGAAACCAAGTGGTTTGCAATCTGGCTCAACAATCCTAAGCAGACAACATACAGATGTTGGTGACCATGAGAAGCTGCGGGTTCCTTCTAAAGGATTTCAAGAGTCTTCTTCCCCAATACATGATAGGAACAAGTCCCTAAGTGGTGATTACAAATACAGCCCAGCTCATGATGGTGAAAGAGTGGCTGATCATTGGGTGCCAATTGGGTGGGCTAGAATTTCAGAAATCGTCCAAACTGTGCAACCTGATGCCAGTTGGGATTCTCAACAGTTTGGGATTGATGATGAAGATGACTTCACTGTTGCAGACTTAGCAGCTCCATATTGGGAGCGTCCAGTGGGGCCTGTTTGGTGGTGCCATGTCTCTGCAGGTCACCCCTCAGTTGATAATTGGCTCCGTAATGCCCAGTGGCTACATCCTGCTGTCAGTTTAGCTTTAAGAGATGAAAGTCGACTGATTAGTGACAGGATGAAACACCTTTTATATGAGGTCCCAGTTAGAGTTTCTGGGGGGTTGTTATTTGAGCTCTTGGGACAGTCAGTTGGTAATCCATTTGTTGACGAGGATGATATACCCATTGTACTTCGTTCCTGGCAAGCACAAAACTTCCTTGTAACTGCTTTGCATGTGAAAGGGCCTGTATCAAGTATAAATGTGTTGGGCATCACAGAAGTTCAGGAACTTCTTTCGACTGGAGGCTATAATGTACCAAGAACAGTGCATGAAGTCATAGCACATCTAACTAGCCGCCTTACTCGATGGGATGATAGGCTATTCCGCAAGTCCATATTTGGTGCCGCAGATGAGATTGAATTGAAGTTTATGAACAGGAGAAACCATGAAGATTTGAATCTTTTTAGCATAATTCTCAACCAAGAAATCAGAAAGTTATCGAGACAGGTCATCAGAGTAAAGTGGTCACTTCATGCAAGAGAGGAGATTTTGTTTGAGCTTATTGAACATTTGAGAGGAAATACTACAAGAAGCTTGTTGGAGAAAATAAGAAAGAGCACAAGAGAAATGATTGAGGAGCAAGAAGCAGTTCGTGGCCGCTTATTTACAATTCAGGATGTGATGCAGAGCACTGTTCGTGCATGGTTGCAGGACAGAAGCCTTAGAGTAACACATAATCTTGCTGTTTTTGGTGGCTGTGGCCTTGTACTTTCCATCATCACTGGGCTTTTTGGAATCAATGTTGATGGAATGCCTGGAGCAAACAATACACCATATGCATTCGGCGTATTTTCAGCTGTTCTCTTCTTTATTGGAATCGTGCTAATTCTACTTGGGTTGCTTTACCTTGGGTTGAAACAGCCTATTGCTGGAGAGCAAGTTGAAGTTAGAAAGATGGAGCTTCAGGAGTTGGTTCAGATGTTTCAGCACGAGGCAGAGACTCATGCCCTGGTTCGTAAAAATGTGGCTCGGAATAATTTACCCCCAACAATGGGAGATGCATTTGCACGTAACGCAAATTATATCCTCATTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

553

Amino Acids

62.34

Weight (kDa)

6.39

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CorA PF01544 349 - 485 9.4e-12 CorA-like Mg2+ transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 530
AccB1I GGYRCC 3 cut(s) 367, 525, 965
AccI GTMKAC 1 cut(s) 622
AciI CCGC 5 cut(s) 247, 919, 949, 969, 1222
AcoI YGGCCR 2 cut(s) 76, 1219
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 1511
AfaI GTAC 3 cut(s) 753, 879, 1332
AfiI CCNNNNNNNGG 4 cut(s) 260, 1493, 1615, 1616
AgsI TTSAA 7 cut(s) 269, 983, 988, 1021, 1130, 1480, 1508
AjnI CCWGG 4 cut(s) 109, 761, 1378, 1572
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 9 cut(s) 244, 335, 488, 605, 697, 1123, 1158, 1421, 1525
AluI AGCT 9 cut(s) 244, 335, 488, 605, 697, 1123, 1158, 1421, 1525
Alw21I GWGCWC 3 cut(s) 699, 1184, 1256
Alw26I GTCTC 3 cut(s) 538, 1060, 1556
AoxI GGCC 6 cut(s) 76, 163, 512, 806, 1219, 1324
ApeKI GCWGC 2 cut(s) 244, 485
ApoI RAATTY 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 1212
AspS9I GGNCC 3 cut(s) 512, 661, 806
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 5 cut(s) 41, 242, 326, 356, 536
AvaII GGWCC 1 cut(s) 661
BalI TGGCCA 1 cut(s) 78
BanI GGYRCC 3 cut(s) 367, 525, 965
BanII GRGCYC 1 cut(s) 699
BauI CACGAG 1 cut(s) 1553
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 3 cut(s) 699, 1184, 1256
BbvI GCAGC 2 cut(s) 231, 497
BccI CCATC 7 cut(s) 68, 125, 335, 924, 1346, 1364, 1513
BciT130I CCWGG 4 cut(s) 111, 763, 1380, 1574
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 3 cut(s) 538, 1060, 1556
BfaI CTAG 2 cut(s) 384, 915
BfmI CTRYAG 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 530
BisI GCNGC 5 cut(s) 245, 486, 919, 969, 1222
BlsI GCNGC 5 cut(s) 246, 487, 920, 970, 1223
Bme1390I CCNGG 5 cut(s) 48, 111, 763, 1380, 1574
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 3 cut(s) 512, 661, 806
BmiI GGNNCC 7 cut(s) 252, 369, 513, 527, 566, 663, 967
BmrFI CCNGG 5 cut(s) 48, 111, 763, 1380, 1574
BmrI ACTGGG 3 cut(s) 571, 659, 1356
BmsI GCATC 4 cut(s) 409, 843, 1236, 1612
BmuI ACTGGG 3 cut(s) 571, 659, 1356
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 3 cut(s) 885, 1401, 1515
Bpu10I CCTNAGC 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 1634
BsaBI GATNNNNATC 1 cut(s) 1020
BsaJI CCNNGG 2 cut(s) 1471, 1572
BsaXI ACNNNNNCTCC 4 cut(s) 1100, 1130, 1488, 1518
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 1493, 1615, 1616
Bse1I ACTGG 6 cut(s) 423, 506, 577, 665, 868, 1351
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 4 cut(s) 111, 763, 1380, 1574
BseDI CCNNGG 2 cut(s) 1471, 1572
BseGI GGATG 6 cut(s) 43, 586, 645, 742, 940, 1246
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 4 cut(s) 260, 1493, 1615, 1616
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 6 cut(s) 423, 506, 577, 665, 868, 1351
BseRI GAGGAG 2 cut(s) 1121, 1214
BseXI GCAGC 2 cut(s) 231, 497
BseYI CCCAGC 1 cut(s) 331
BshFI GGCC 6 cut(s) 78, 165, 514, 808, 1221, 1326
BshNI GGYRCC 3 cut(s) 367, 525, 965
BsiHKAI GWGCWC 3 cut(s) 699, 1184, 1256
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 3 cut(s) 289, 647, 717
BslI CCNNNNNNNGG 4 cut(s) 260, 1493, 1615, 1616
BsmAI GTCTC 3 cut(s) 538, 1060, 1556
BsmFI GGGAC 3 cut(s) 289, 647, 717
BsmI GAATGC 2 cut(s) 1380, 1403
BsnI GGCC 6 cut(s) 78, 165, 514, 808, 1221, 1326
Bsp1286I GDGCHC 3 cut(s) 699, 1184, 1256
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 5 cut(s) 247, 919, 949, 969, 1222
BspANI GGCC 6 cut(s) 78, 165, 514, 808, 1221, 1326
BspCNI CTCAG 1 cut(s) 563
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 7 cut(s) 252, 369, 513, 527, 566, 663, 967
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 530
BspT107I GGYRCC 3 cut(s) 367, 525, 965
BsrI ACTGG 6 cut(s) 423, 506, 577, 665, 868, 1351
BssECI CCNNGG 2 cut(s) 1471, 1572
BssMI GATC 1 cut(s) 358
BssSI CACGAG 1 cut(s) 1553
BssT1I CCWWGG 1 cut(s) 1471
Bst2BI CACGAG 1 cut(s) 1553
Bst2UI CCWGG 4 cut(s) 111, 763, 1380, 1574
Bst4CI ACNGT 6 cut(s) 409, 441, 472, 708, 889, 1258
Bst6I CTCTTC 1 cut(s) 1433
BstBAI YACGTR 1 cut(s) 1634
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 5 cut(s) 206, 308, 481, 550, 1286
BstEII GGTNACC 2 cut(s) 230, 542
BstENI CCTNNNNNAGG 1 cut(s) 258
BstF5I GGATG 6 cut(s) 43, 586, 645, 742, 940, 1246
BstKTI GATC 1 cut(s) 361
BstMAI GTCTC 3 cut(s) 538, 1060, 1556
BstMBI GATC 1 cut(s) 358
BstMWI GCNNNNNNNGC 2 cut(s) 482, 1637
BstNI CCWGG 4 cut(s) 111, 763, 1380, 1574
BstNSI RCATGY 1 cut(s) 800
BstPI GGTNACC 2 cut(s) 230, 542
BstSCI CCNGG 5 cut(s) 46, 109, 761, 1378, 1572
BstSFI CTRYAG 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 231, 497
BstV2I GAAGAC 1 cut(s) 267
BstXI CCANNNNNNTGG 2 cut(s) 86, 378
BsuRI GGCC 6 cut(s) 78, 165, 514, 808, 1221, 1326
BtsCI GGATG 6 cut(s) 43, 586, 645, 742, 940, 1246
BtsIMutI CAGTG 6 cut(s) 468, 513, 584, 894, 1254, 1344
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 1 cut(s) 768
CciI TCATGA 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 512, 661, 806
CseI GACGC 1 cut(s) 491
Csp6I GTAC 3 cut(s) 752, 878, 1331
CviQI GTAC 3 cut(s) 752, 878, 1331
DdeI CTNAG 5 cut(s) 206, 308, 481, 550, 1286
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 76, 1219
Eam1104I CTCTTC 1 cut(s) 1433
EarI CTCTTC 1 cut(s) 1433
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 1471
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 661
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 1511
Eco91I GGTNACC 2 cut(s) 230, 542
EcoICRI GAGCTC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 258
EcoO109I RGGNCCY 3 cut(s) 512, 661, 806
EcoO65I GGTNACC 2 cut(s) 230, 542
EcoRII CCWGG 4 cut(s) 109, 761, 1378, 1572
EcoT14I CCWWGG 1 cut(s) 1471
EcoT22I ATGCAT 2 cut(s) 1405, 1627
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 1471
FaqI GGGAC 3 cut(s) 289, 647, 717
FauI CCCGC 1 cut(s) 240
FauNDI CATATG 1 cut(s) 1399
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 245, 486, 919, 969, 1222
FokI GGATG 6 cut(s) 30, 573, 652, 749, 947, 1253
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 5 cut(s) 245, 486, 919, 969, 1222
FspBI CTAG 2 cut(s) 384, 915
GluI GCNGC 5 cut(s) 245, 486, 919, 969, 1222
GsaI CCCAGC 1 cut(s) 335
GsuI CTGGAG 3 cut(s) 885, 1401, 1515
HaeIII GGCC 6 cut(s) 78, 165, 514, 808, 1221, 1326
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 491
HincII GTYRAC 2 cut(s) 623, 730
HindII GTYRAC 2 cut(s) 623, 730
HindIII AAGCTT 1 cut(s) 1156
HinfI GANTC 6 cut(s) 272, 431, 1021, 1359, 1440, 1564
HpaII CCGG 1 cut(s) 48
HphI GGTGA 5 cut(s) 41, 242, 326, 356, 536
Hpy166II GTNNAC 3 cut(s) 119, 623, 730
Hpy188I TCNGA 5 cut(s) 394, 1055, 1079, 1542, 1596
Hpy188III TCNNGA 6 cut(s) 269, 338, 848, 1065, 1238, 1529
Hpy8I GTNNAC 3 cut(s) 119, 623, 730
HpyAV CCTTC 1 cut(s) 264
HpyCH4III ACNGT 6 cut(s) 409, 441, 472, 708, 889, 1258
HpyCH4IV ACGT 1 cut(s) 1633
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 1637
HpyF3I CTNAG 5 cut(s) 206, 308, 481, 550, 1286
HpySE526I ACGT 1 cut(s) 1633
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 1 cut(s) 358
LmnI GCTCC 6 cut(s) 493, 499, 570, 1201, 1382, 1522
Lsp1109I GCAGC 2 cut(s) 231, 497
LweI GCATC 4 cut(s) 409, 843, 1236, 1612
MaeI CTAG 2 cut(s) 384, 915
MaeII ACGT 1 cut(s) 1633
MaeIII GTNAC 9 cut(s) 20, 29, 230, 542, 632, 784, 1089, 1291, 1634
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 6 cut(s) 76, 267, 270, 470, 1025, 1420
MfeI CAATTG 1 cut(s) 372
MhlI GDGCHC 3 cut(s) 699, 1184, 1256
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 9 cut(s) 372, 387, 559, 983, 1035, 1233, 1449, 1600, 1642
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 2 cut(s) 281, 1558
MmeI TCCRAC 1 cut(s) 1143
Mox20I TGGCCA 1 cut(s) 78
Mph1103I ATGCAT 2 cut(s) 1405, 1627
MroXI GAANNNNTTC 1 cut(s) 1212
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 608
MslI CAYNNNNRTG 3 cut(s) 84, 222, 342
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 1421
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 5 cut(s) 48, 111, 763, 1380, 1574
MunI CAATTG 1 cut(s) 372
Mva1269I GAATGC 2 cut(s) 1380, 1403
MvaI CCWGG 4 cut(s) 111, 763, 1380, 1574
MwoI GCNNNNNNNGC 2 cut(s) 482, 1637
NciI CCSGG 1 cut(s) 48
NdeI CATATG 1 cut(s) 1399
NdeII GATC 1 cut(s) 358
NlaIV GGNNCC 7 cut(s) 252, 369, 513, 527, 566, 663, 967
NmuCI GTSAC 5 cut(s) 29, 230, 542, 632, 1089
NsiI ATGCAT 2 cut(s) 1405, 1627
NspI RCATGY 1 cut(s) 800
PagI TCATGA 1 cut(s) 337
PctI GAATGC 2 cut(s) 1380, 1403
PdmI GAANNNNTTC 1 cut(s) 1212
PfeI GAWTC 4 cut(s) 431, 1021, 1359, 1440
PkrI GCNGC 5 cut(s) 246, 487, 920, 970, 1223
PleI GAGTC 2 cut(s) 280, 1558
PpsI GAGTC 2 cut(s) 280, 1558
Ppu21I YACGTR 1 cut(s) 1634
PpuMI RGGWCCY 1 cut(s) 661
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 661
Psp6I CCWGG 4 cut(s) 109, 761, 1378, 1572
PspEI GGTNACC 2 cut(s) 230, 542
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 4 cut(s) 109, 761, 1378, 1572
PspN4I GGNNCC 7 cut(s) 252, 369, 513, 527, 566, 663, 967
PspPI GGNCC 3 cut(s) 512, 661, 806
PspPPI RGGWCCY 1 cut(s) 661
PstI CTGCAG 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 3 cut(s) 753, 879, 1332
RsaNI GTAC 3 cut(s) 752, 878, 1331
RseI CAYNNNNRTG 3 cut(s) 84, 222, 342
SacI GAGCTC 1 cut(s) 699
SalI GTCGAC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 5 cut(s) 245, 486, 919, 969, 1222
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 3 cut(s) 512, 661, 806
SchI GAGTC 2 cut(s) 281, 1558
ScrFI CCNGG 5 cut(s) 48, 111, 763, 1380, 1574
SduI GDGCHC 3 cut(s) 699, 1184, 1256
SfaNI GCATC 4 cut(s) 409, 843, 1236, 1612
SfcI CTRYAG 1 cut(s) 537
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 3 cut(s) 84, 222, 342
Sse9I AATT 9 cut(s) 372, 387, 559, 983, 1035, 1233, 1449, 1600, 1642
SsiI CCGC 5 cut(s) 247, 919, 949, 969, 1222
SspMI CTAG 2 cut(s) 384, 915
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 5 cut(s) 46, 109, 761, 1378, 1572
StyI CCWWGG 1 cut(s) 1471
TaaI ACNGT 6 cut(s) 409, 441, 472, 708, 889, 1258
TaiI ACGT 1 cut(s) 1636
TaqI TCGA 5 cut(s) 160, 622, 860, 928, 1064
TasI AATT 9 cut(s) 372, 387, 559, 983, 1035, 1233, 1449, 1600, 1642
TatI WGTACW 2 cut(s) 751, 1330
TauI GCSGC 3 cut(s) 921, 971, 1224
TfiI GAWTC 4 cut(s) 431, 1021, 1359, 1440
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 6 cut(s) 475, 513, 584, 894, 1261, 1351
TseFI GTSAC 5 cut(s) 29, 230, 542, 632, 1089
TseI GCWGC 2 cut(s) 244, 485
Tsp45I GTSAC 5 cut(s) 29, 230, 542, 632, 1089
TspDTI ATGAA 8 cut(s) 145, 471, 630, 656, 909, 1010, 1026, 1085
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 6 cut(s) 475, 513, 584, 894, 1261, 1351
VpaK11BI GGWCC 1 cut(s) 661
XagI CCTNNNNNAGG 1 cut(s) 258
XapI RAATTY 1 cut(s) 387
XceI RCATGY 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 1212
XspI CTAG 2 cut(s) 384, 915
Zsp2I ATGCAT 2 cut(s) 1405, 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.