FvH4_3g16060

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008387
Physical Location & Seq
Reverse (-)
10109890 .. 10114292
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16060.t1

Sequence Viewer

Length: 1113 bp
ATGAATATGAAAGGCACCAAGGTTGCAGTGGTGGGGAGTGGAATTTCAGGAGCTGTGTGCGCTTCAAGTCTTGCCAGAAATGGAATCTCTGTGACATTGTTCGAGTCTGCTAGAGGCCCAGGTGGCCGTATGTCCCAAAGAAGAGAAAATGTTGAAGATGGGAAGGAGCTGTTGTTTGACCATGGTGCTCCGTTTTTCACTGCCAACAACACCGAGGTTCAGAGTCTTGTCCATGACTGGGAATCAAAGGGTCTGGCAGCTATCTGGAAAGAGAAATTTGGTTGTTTTGATCGCACGTCAAACAAATTTGTTGACCTAGAACAGGAAGGATTAATAAGTAAGAGATATGTGGGTATCCCAGGGATGAATTCTGTATGCAAAGCATTGTGCCGTGAACCTGGTGTGGAAAGTAAGTTTGGTACAAGTGTTAATAGATTGGAGTGGCTAGAGGATCAAAATATGTGGTCACTGATTGGTTTGGATGGACAAAATCTTGGTCAGTTTAAGGGAGTGGTTGCAACAGATAAAAACATAGTTTCACCCAGGTTTACAAGTGTTACAGGAAGACAACCACCTCTTGATTTGAAATTATTTCCAGAGTTAGCAGTTAAGCTGAATGACATTCCGGTTCGGCCATGCTTTGCTCTAATGATAGCCTTTGCAGAACCTCTGACATCGATACCCTTTAAAGGGTTCTCAATTAAAAATTCTGAAGTTTTAAGTTGGGCTCATTGTGACAGCAGCAAGCCTGGCCGTTCTACCTCTAGTGAGCGATGGGTTCTGCATTCTACAATGGAGTATGCTCAGAGAATAATTGCTGAAACTGGACTTCAGAAGCTTTCAGATGCAACATTGACTAAAGTGGCTGAAGAACTATTCCAAGAATTACAAAGCCTGGGACTTATTATTTCTCAACCCTTTTTTAAGAAAGCTCATAGATGGGGAAGCGCTTTTCCAGCTACTACCATAGCCAGAGAGGAGAAATGTCTTTGGGATGGGAACAAGAGACTTGCCATCTGTGGTGATTTCTGTGTTAGTCCAGATGTTGAAGGTGCTATAGTTAGTGGCCTGGCCGCAGCTTCCCAACTTTCAGAGCTCTTATGCTGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

371

Amino Acids

40.47

Weight (kDa)

6.95

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 7 - 45 2.5e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 8 - 157 3.3e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 10 - 79 2.4e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 15 - 79 5e-07 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 1 cut(s) 1074
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 4 cut(s) 124, 632, 751, 1071
AcsI RAATTY 5 cut(s) 42, 275, 305, 367, 706
AcuI CTGAAG 3 cut(s) 732, 815, 888
AfaI GTAC 1 cut(s) 421
AfeI AGCGCT 1 cut(s) 949
AfiI CCNNNNNNNGG 4 cut(s) 238, 322, 689, 690
AgsI TTSAA 4 cut(s) 66, 155, 586, 1049
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 9 cut(s) 53, 169, 260, 613, 838, 932, 959, 1079, 1096
AluI AGCT 9 cut(s) 53, 169, 260, 613, 838, 932, 959, 1079, 1096
Alw21I GWGCWC 2 cut(s) 190, 1098
Alw26I GTCTC 1 cut(s) 1000
AlwI GGATC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 53
Aor51HI AGCGCT 1 cut(s) 949
AoxI GGCC 6 cut(s) 115, 124, 632, 751, 1066, 1071
ApeKI GCWGC 4 cut(s) 257, 741, 1076, 1104
ApoI RAATTY 5 cut(s) 42, 275, 305, 367, 706
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
AseI ATTAAT 1 cut(s) 332
AspLEI GCGC 2 cut(s) 62, 950
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 531, 1034
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 2 cut(s) 730, 1098
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 2 cut(s) 190, 1098
BbvI GCAGC 4 cut(s) 269, 753, 1088, 1091
BccI CCATC 6 cut(s) 152, 476, 768, 933, 989, 1022
BceAI ACGGC 3 cut(s) 111, 375, 738
BciT130I CCWGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
BciVI GTATCC 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 1000
BfaI CTAG 4 cut(s) 111, 317, 446, 765
BfmI CTRYAG 1 cut(s) 1056
BfoI RGCGCY 1 cut(s) 951
BfuI GTATCC 1 cut(s) 365
BglI GCCNNNNNGGC 1 cut(s) 123
BisI GCNGC 5 cut(s) 258, 742, 1074, 1077, 1105
BlsI GCNGC 5 cut(s) 259, 743, 1075, 1078, 1106
Bme1390I CCNGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 1 cut(s) 835
BmuI ACTGGG 1 cut(s) 247
BpiI GAAGAC 1 cut(s) 571
Bsa29I ATCGAT 1 cut(s) 677
BsaJI CCNNGG 8 cut(s) 18, 118, 181, 213, 358, 359, 542, 895
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 322, 689, 690
Bse1I ACTGG 2 cut(s) 242, 829
BseBI CCWGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 8 cut(s) 18, 118, 181, 213, 358, 359, 542, 895
BseGI GGATG 3 cut(s) 369, 487, 1000
BseLI CCNNNNNNNGG 4 cut(s) 238, 322, 689, 690
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 2 cut(s) 242, 829
BseRI GAGGAG 1 cut(s) 992
BseXI GCAGC 4 cut(s) 269, 753, 1088, 1091
BshFI GGCC 6 cut(s) 117, 126, 634, 753, 1068, 1073
BshNI GGYRCC 1 cut(s) 14
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 190, 1098
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 2 cut(s) 118, 912
BslI CCNNNNNNNGG 4 cut(s) 238, 322, 689, 690
BsmAI GTCTC 1 cut(s) 1000
BsmFI GGGAC 2 cut(s) 118, 912
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 6 cut(s) 117, 126, 634, 753, 1068, 1073
Bsp1286I GDGCHC 3 cut(s) 190, 730, 1098
Bsp143I GATC 2 cut(s) 289, 451
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 1 cut(s) 1074
BspANI GGCC 6 cut(s) 117, 126, 634, 753, 1068, 1073
BspCNI CTCAG 1 cut(s) 817
BspDI ATCGAT 1 cut(s) 677
BspLI GGNNCC 1 cut(s) 16
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 14
BsrI ACTGG 2 cut(s) 242, 829
BssECI CCNNGG 8 cut(s) 18, 118, 181, 213, 358, 359, 542, 895
BssMI GATC 2 cut(s) 289, 451
BssT1I CCWWGG 2 cut(s) 18, 181
Bst2UI CCWGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
Bst6I CTCTTC 1 cut(s) 136
BstC8I GCNNGC 1 cut(s) 746
BstDEI CTNAG 1 cut(s) 804
BstDSI CCRYGG 1 cut(s) 181
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 3 cut(s) 369, 487, 1000
BstH2I RGCGCY 1 cut(s) 951
BstHHI GCGC 2 cut(s) 62, 950
BstKTI GATC 2 cut(s) 292, 454
BstMAI GTCTC 1 cut(s) 1000
BstMBI GATC 2 cut(s) 289, 451
BstMWI GCNNNNNNNGC 4 cut(s) 59, 123, 750, 956
BstNI CCWGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
BstSCI CCNGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
BstSFI CTRYAG 1 cut(s) 1056
BstV1I GCAGC 4 cut(s) 269, 753, 1088, 1091
BstV2I GAAGAC 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 677
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 6 cut(s) 117, 126, 634, 753, 1068, 1073
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 787
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 3 cut(s) 369, 487, 1000
BtsI GCAGTG 2 cut(s) 33, 198
BtsIMutI CAGTG 3 cut(s) 33, 198, 467
Cac8I GCNNGC 1 cut(s) 746
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 2 cut(s) 62, 950
Cfr13I GGNCC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 677
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 1 cut(s) 420
CspCI CAANNNNNGTGG 2 cut(s) 443, 478
CviAII CATG 3 cut(s) 182, 233, 636
CviQI GTAC 1 cut(s) 420
DdeI CTNAG 1 cut(s) 804
DpnI GATC 2 cut(s) 291, 453
DpnII GATC 2 cut(s) 289, 451
DraI TTTAAA 1 cut(s) 688
EaeI YGGCCR 4 cut(s) 124, 632, 751, 1071
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 2 cut(s) 18, 181
Eco24I GRGCYC 2 cut(s) 730, 1098
Eco47III AGCGCT 1 cut(s) 949
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 3 cut(s) 732, 815, 888
EcoICRI GAGCTC 1 cut(s) 1096
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
EcoT14I CCWWGG 2 cut(s) 18, 181
EcoT38I GRGCYC 2 cut(s) 730, 1098
ErhI CCWWGG 2 cut(s) 18, 181
FaeI CATG 3 cut(s) 185, 236, 639
FaqI GGGAC 2 cut(s) 118, 912
FatI CATG 3 cut(s) 181, 232, 635
Fnu4HI GCNGC 5 cut(s) 258, 742, 1074, 1077, 1105
FokI GGATG 3 cut(s) 376, 494, 1007
FriOI GRGCYC 2 cut(s) 730, 1098
Fsp4HI GCNGC 5 cut(s) 258, 742, 1074, 1077, 1105
FspBI CTAG 4 cut(s) 111, 317, 446, 765
GlaI GCGC 2 cut(s) 61, 949
GluI GCNGC 5 cut(s) 258, 742, 1074, 1077, 1105
HaeII RGCGCY 1 cut(s) 951
HaeIII GGCC 6 cut(s) 117, 126, 634, 753, 1068, 1073
HapII CCGG 1 cut(s) 626
HhaI GCGC 2 cut(s) 62, 950
Hin1II CATG 3 cut(s) 185, 236, 639
Hin6I GCGC 2 cut(s) 60, 948
HinP1I GCGC 2 cut(s) 60, 948
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 4 cut(s) 84, 104, 223, 242
HpaII CCGG 1 cut(s) 626
HphI GGTGA 2 cut(s) 531, 1034
Hpy166II GTNNAC 3 cut(s) 313, 395, 549
Hpy188I TCNGA 7 cut(s) 222, 672, 712, 807, 834, 844, 1093
Hpy188III TCNNGA 5 cut(s) 48, 265, 578, 596, 1040
Hpy8I GTNNAC 3 cut(s) 313, 395, 549
HpyAV CCTTC 3 cut(s) 157, 320, 1043
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 6 cut(s) 26, 378, 518, 662, 784, 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 123, 750, 956
HpyF3I CTNAG 1 cut(s) 804
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 3 cut(s) 185, 236, 639
HspAI GCGC 2 cut(s) 60, 948
Kzo9I GATC 2 cut(s) 289, 451
LmnI GCTCC 3 cut(s) 50, 166, 193
Lsp1109I GCAGC 4 cut(s) 269, 753, 1088, 1091
LweI GCATC 1 cut(s) 835
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 4 cut(s) 111, 317, 446, 765
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 4 cut(s) 91, 465, 556, 734
MalI GATC 2 cut(s) 291, 453
MboI GATC 2 cut(s) 289, 451
MboII GAAGA 4 cut(s) 153, 167, 576, 881
MhlI GDGCHC 3 cut(s) 190, 730, 1098
MluCI AATT 9 cut(s) 42, 275, 305, 367, 587, 699, 706, 813, 884
MlyI GAGTC 2 cut(s) 113, 232
MnlI CCTC 7 cut(s) 107, 208, 442, 585, 678, 772, 970
MseI TTAA 8 cut(s) 332, 429, 504, 609, 687, 702, 719, 924
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
MwoI GCNNNNNNNGC 4 cut(s) 59, 123, 750, 956
NcoI CCATGG 1 cut(s) 181
NdeII GATC 2 cut(s) 289, 451
NlaIII CATG 3 cut(s) 185, 236, 639
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 3 cut(s) 91, 465, 734
PasI CCCWGGG 1 cut(s) 359
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 2 cut(s) 84, 242
PkrI GCNGC 5 cut(s) 259, 743, 1075, 1078, 1106
PleI GAGTC 2 cut(s) 112, 231
PpsI GAGTC 2 cut(s) 112, 231
PshBI ATTAAT 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 1098
Psp6I CCWGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
PspGI CCWGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
SacI GAGCTC 1 cut(s) 1098
SaqAI TTAA 8 cut(s) 332, 429, 504, 609, 687, 702, 719, 924
SatI GCNGC 5 cut(s) 258, 742, 1074, 1077, 1105
Sau3AI GATC 2 cut(s) 289, 451
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 2 cut(s) 113, 232
ScrFI CCNGG 7 cut(s) 120, 360, 399, 544, 750, 896, 1070
SduI GDGCHC 3 cut(s) 190, 730, 1098
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 1 cut(s) 835
SfcI CTRYAG 1 cut(s) 1056
SfiI GGCCNNNNNGGCC 1 cut(s) 123
Sse9I AATT 9 cut(s) 42, 275, 305, 367, 587, 699, 706, 813, 884
SsiI CCGC 1 cut(s) 1074
SspMI CTAG 4 cut(s) 111, 317, 446, 765
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 7 cut(s) 118, 358, 397, 542, 748, 894, 1068
StyI CCWWGG 2 cut(s) 18, 181
TaiI ACGT 1 cut(s) 299
TaqI TCGA 2 cut(s) 102, 677
TasI AATT 9 cut(s) 42, 275, 305, 367, 587, 699, 706, 813, 884
TauI GCSGC 1 cut(s) 1076
TfiI GAWTC 2 cut(s) 84, 242
Tru1I TTAA 8 cut(s) 332, 429, 504, 609, 687, 702, 719, 924
Tru9I TTAA 8 cut(s) 332, 429, 504, 609, 687, 702, 719, 924
TscAI CASTG 3 cut(s) 33, 205, 474
TseFI GTSAC 3 cut(s) 91, 465, 734
TseI GCWGC 4 cut(s) 257, 741, 1076, 1104
Tsp45I GTSAC 3 cut(s) 91, 465, 734
TspDTI ATGAA 3 cut(s) 17, 23, 380
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 3 cut(s) 33, 205, 474
VspI ATTAAT 1 cut(s) 332
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 5 cut(s) 42, 275, 305, 367, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 4 cut(s) 111, 317, 446, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.