RLG00000032980

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008387
Physical Location & Seq
Reverse (-)
21659165 .. 21663027
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032980

Sequence Viewer

Length: 1107 bp
ATGAAAGGCACTAAGGTTGCAGTGGTGGGAAGTGGAATTTCAGGAGCTGTGTGCGCATCAAGTCTTGCCAGAAATGGACTCTCTGTGACACTGTTCGAGTCTGCTAGAGGCCCAGGTGGCCGCATGTCTCAAAGAAGAGAAAATGTTGAAGATGGAAATGAGCTATTGTTTGACCATGGTGCTCCGTTTTTCACTGCCAACAACACCGAGGTTCTGAGTCTTGTCCATGAGTGGGAATCAAAGGGTCTGGCAGCTATCTGGAAAGAGAAATTTGGTTCTTTTGATCGCACGTCGAACAAATTTGTTGACCTAGAACAGGAAGGATTAATAAGTAAGAGATATGTGGGTATCCCAGGGATGAATTCTGTGTGCAGGGCATTATGCCGGGAGCCTGGTGTTGAAAGTAAGTTTGGGACAAGTGTTGGAAGATTGGAGTGGCTAGAGGATCAAAATTTGTGGTCATTGATTGGTTTGGATGGACAAAACCTTGGTCAGTTTAAGGGAGTGGTCGCAACAGACAAAAACATAGTTTCACCCAGGTTTACAAGTGTTACAGGAAAACCACTACCTCTTGATTTGAAATTATTTCCAGAGTTAGCAGTTAAGTTGAATGACATTCCTGTTCGTCCGTGCTTTGCTCTAATGATAGCCTTTGCAGAACCTCTGACATCGATACCCTTTAAAGGCTTCTCAATAAAAAATTCTGAAGTTTTAAGCTGGGCTCATTGTGACAGCAGCAAGCCTGGCCGTTCTACCTCTAGTGAGCGATGGGTTCTGCATTCTACAATGGAGTATGCTCAGAGAATAATTGCTCAAACTGGACTTCAGAAGCTTTCAGATGCAACATTGACCAAAGTGGCTGAAGAACTATTCCAAGAATTACAAAGCCTGGGACTTAATATTTCACAGCCCTTTTTCAAGAAAGCTCATAGATGGGGAAGTGCTTTTCCAGCTACAAGCATAGCTAGAGAGGAGAAATGTCTCTGGGATGGGAACAAGAGGTTTGCCATCTGCGGTGATTTCTGTGTTAGTCCAGATGTTGAAGGTGCTATAGTTAGTGGCCTGGCCGCAGCTTCCAAACTTTCAGAGCTCTTCAGCTGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

369

Amino Acids

40.15

Weight (kDa)

7.54

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 5 - 43 1.6e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 6 - 146 2.1e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 8 - 77 2.4e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 13 - 77 1.4e-07 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AciI CCGC 3 cut(s) 121, 1014, 1068
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 3 cut(s) 118, 745, 1065
AcsI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
AcuI CTGAAG 4 cut(s) 726, 809, 882, 1078
AfiI CCNNNNNNNGG 3 cut(s) 232, 316, 683
AgsI TTSAA 6 cut(s) 149, 401, 580, 610, 919, 1043
AjiI CACGTC 1 cut(s) 291
AjnI CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
Alw21I GWGCWC 2 cut(s) 184, 1092
Alw26I GTCTC 2 cut(s) 132, 986
AlwI GGATC 1 cut(s) 453
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 5 cut(s) 109, 118, 745, 1060, 1065
ApeKI GCWGC 4 cut(s) 251, 735, 1070, 1098
ApoI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AseI ATTAAT 1 cut(s) 326
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 2 cut(s) 525, 1028
BanII GRGCYC 2 cut(s) 724, 1092
Bbv12I GWGCWC 2 cut(s) 184, 1092
BbvI GCAGC 4 cut(s) 263, 747, 1082, 1085
BccI CCATC 6 cut(s) 146, 470, 762, 927, 983, 1016
BceAI ACGGC 1 cut(s) 732
BciT130I CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BciVI GTATCC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 2 cut(s) 132, 986
BfaI CTAG 5 cut(s) 105, 311, 440, 759, 966
BfmI CTRYAG 1 cut(s) 1050
BfuI GTATCC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
BlsI GCNGC 6 cut(s) 122, 253, 737, 1069, 1072, 1100
Bme1390I CCNGG 8 cut(s) 114, 354, 386, 393, 538, 744, 890, 1064
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 8 cut(s) 114, 354, 386, 393, 538, 744, 890, 1064
BmsI GCATC 2 cut(s) 65, 829
BpuMI CCSGG 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 671
BsaJI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 487, 536, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 316, 683
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 487, 536, 889
BseGI GGATG 3 cut(s) 363, 481, 994
BseLI CCNNNNNNNGG 3 cut(s) 232, 316, 683
BseMII CTCAG 2 cut(s) 206, 812
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 4 cut(s) 263, 747, 1082, 1085
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 391
BshFI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BshVI ATCGAT 1 cut(s) 671
BsiHKAI GWGCWC 2 cut(s) 184, 1092
BsiSI CCGG 1 cut(s) 385
BslFI GGGAC 2 cut(s) 427, 906
BslI CCNNNNNNNGG 3 cut(s) 232, 316, 683
BsmAI GTCTC 2 cut(s) 132, 986
BsmFI GGGAC 2 cut(s) 427, 906
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
Bsp1286I GDGCHC 3 cut(s) 184, 724, 1092
Bsp143I GATC 2 cut(s) 283, 445
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 3 cut(s) 121, 1014, 1068
BspANI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BspCNI CTCAG 2 cut(s) 207, 811
BspDI ATCGAT 1 cut(s) 671
BspLI GGNNCC 1 cut(s) 390
BspPI GGATC 1 cut(s) 453
BspQI GCTCTTC 1 cut(s) 1097
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 487, 536, 889
BssMI GATC 2 cut(s) 283, 445
BssT1I CCWWGG 2 cut(s) 175, 487
Bst2UI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 2 cut(s) 130, 1097
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 3 cut(s) 12, 215, 798
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 3 cut(s) 363, 481, 994
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 286, 448
BstMAI GTCTC 2 cut(s) 132, 986
BstMBI GATC 2 cut(s) 283, 445
BstMWI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
BstNI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 8 cut(s) 112, 352, 384, 391, 536, 742, 888, 1062
BstSFI CTRYAG 1 cut(s) 1050
BstV1I GCAGC 4 cut(s) 263, 747, 1082, 1085
Bsu15I ATCGAT 1 cut(s) 671
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 781
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 3 cut(s) 363, 481, 994
BtsI GCAGTG 2 cut(s) 27, 192
BtsIMutI CAGTG 3 cut(s) 27, 89, 192
Cac8I GCNNGC 1 cut(s) 740
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 671
CspCI CAANNNNNGTGG 2 cut(s) 437, 472
CviAII CATG 3 cut(s) 124, 176, 227
DdeI CTNAG 3 cut(s) 12, 215, 798
DpnI GATC 2 cut(s) 285, 447
DpnII GATC 2 cut(s) 283, 445
DraI TTTAAA 1 cut(s) 682
EaeI YGGCCR 3 cut(s) 118, 745, 1065
Eam1104I CTCTTC 2 cut(s) 130, 1097
EarI CTCTTC 2 cut(s) 130, 1097
Ecl136II GAGCTC 1 cut(s) 1090
Eco130I CCWWGG 2 cut(s) 175, 487
Eco24I GRGCYC 2 cut(s) 724, 1092
Eco53kI GAGCTC 1 cut(s) 1090
Eco57I CTGAAG 4 cut(s) 726, 809, 882, 1078
EcoICRI GAGCTC 1 cut(s) 1090
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
EcoT14I CCWWGG 2 cut(s) 175, 487
EcoT38I GRGCYC 2 cut(s) 724, 1092
ErhI CCWWGG 2 cut(s) 175, 487
FaeI CATG 3 cut(s) 127, 179, 230
FaqI GGGAC 2 cut(s) 427, 906
FatI CATG 3 cut(s) 123, 175, 226
Fnu4HI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
FokI GGATG 3 cut(s) 370, 488, 1001
FriOI GRGCYC 2 cut(s) 724, 1092
Fsp4HI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
FspAI RTGCGCAY 1 cut(s) 55
FspBI CTAG 5 cut(s) 105, 311, 440, 759, 966
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
GsaI CCCAGC 1 cut(s) 721
HaeIII GGCC 5 cut(s) 111, 120, 747, 1062, 1067
HapII CCGG 1 cut(s) 385
HhaI GCGC 1 cut(s) 56
Hin1II CATG 3 cut(s) 127, 179, 230
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 4 cut(s) 78, 98, 217, 236
HpaII CCGG 1 cut(s) 385
HphI GGTGA 2 cut(s) 525, 1028
Hpy166II GTNNAC 2 cut(s) 307, 543
Hpy188I TCNGA 7 cut(s) 216, 666, 706, 801, 828, 838, 1087
Hpy188III TCNNGA 6 cut(s) 42, 259, 572, 590, 919, 1034
Hpy8I GTNNAC 2 cut(s) 307, 543
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 2 cut(s) 314, 1037
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 5 cut(s) 20, 372, 656, 778, 842
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
HpyF3I CTNAG 3 cut(s) 12, 215, 798
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 127, 179, 230
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 283, 445
LguI GCTCTTC 1 cut(s) 1097
LmnI GCTCC 3 cut(s) 44, 187, 388
Lsp1109I GCAGC 4 cut(s) 263, 747, 1082, 1085
LweI GCATC 2 cut(s) 65, 829
MaeI CTAG 5 cut(s) 105, 311, 440, 759, 966
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 3 cut(s) 85, 550, 728
MalI GATC 2 cut(s) 285, 447
MboI GATC 2 cut(s) 283, 445
MboII GAAGA 5 cut(s) 147, 161, 438, 875, 1084
MhlI GDGCHC 3 cut(s) 184, 724, 1092
MluCI AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
MlyI GAGTC 3 cut(s) 72, 107, 226
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 8 cut(s) 101, 202, 436, 579, 672, 766, 964, 993
MseI TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
MspA1I CMGCKG 1 cut(s) 1098
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 8 cut(s) 114, 354, 386, 393, 538, 744, 890, 1064
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
MwoI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
NciI CCSGG 1 cut(s) 386
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 283, 445
NlaIII CATG 3 cut(s) 127, 179, 230
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 85, 728
NsbI TGCGCA 1 cut(s) 55
NspI RCATGY 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 353
PciSI GCTCTTC 1 cut(s) 1097
PctI GAATGC 1 cut(s) 778
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 6 cut(s) 122, 253, 737, 1069, 1072, 1100
PleI GAGTC 3 cut(s) 72, 106, 225
PpsI GAGTC 3 cut(s) 72, 106, 225
PshBI ATTAAT 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 1092
Psp6I CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 1098
SacI GAGCTC 1 cut(s) 1092
SapI GCTCTTC 1 cut(s) 1097
SaqAI TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
SatI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
Sau3AI GATC 2 cut(s) 283, 445
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 3 cut(s) 72, 107, 226
ScrFI CCNGG 8 cut(s) 114, 354, 386, 393, 538, 744, 890, 1064
SduI GDGCHC 3 cut(s) 184, 724, 1092
SfaNI GCATC 2 cut(s) 65, 829
SfcI CTRYAG 1 cut(s) 1050
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sse9I AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
SsiI CCGC 3 cut(s) 121, 1014, 1068
SspI AATATT 1 cut(s) 901
SspMI CTAG 5 cut(s) 105, 311, 440, 759, 966
SstI GAGCTC 1 cut(s) 1092
StyD4I CCNGG 8 cut(s) 112, 352, 384, 391, 536, 742, 888, 1062
StyI CCWWGG 2 cut(s) 175, 487
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 96, 293, 671
TasI AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
TauI GCSGC 2 cut(s) 123, 1070
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
Tru9I TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
TscAI CASTG 3 cut(s) 27, 96, 199
TseFI GTSAC 2 cut(s) 85, 728
TseI GCWGC 4 cut(s) 251, 735, 1070, 1098
Tsp45I GTSAC 2 cut(s) 85, 728
TspDTI ATGAA 2 cut(s) 17, 374
TspGWI ACGGA 2 cut(s) 174, 618
TspRI CASTG 3 cut(s) 27, 96, 199
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
XceI RCATGY 1 cut(s) 127
XspI CTAG 5 cut(s) 105, 311, 440, 759, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.