MD05G1215100.v1.1

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008387
Physical Location & Seq
Forward (+)
34611353 .. 34615152
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1215100.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAATACCACTAAAGTTGCGGTGGTGGGAAGTGGAATTGCAGGAGCAGTGTGTGCATCAACTCTCGCCAGAAATGGAGTGTCTGTAACTTTGTTCGAGTCTGCCAGAGGCCCCGGCGGCCGCATGTCTCAGAGAAGAGAAGTTGTTGAAGATGAGAAGGAGCTATTGTTTGACCATGGTGCTCCATTTTTCAATGCCAACAAAACCGAGGTTCTCGGTCTTGTTCGTGAATGGGAATCAAAGGGTCTTGTTGCTTGCTGGAAAGAGAAATTTGGCTTCTTTGATCGCATTTCCAACAAATTTTTTGACCTTGAACAGGAAGGATTAATAAGTAAGAGATATGTGGGTATGCCAGGCATGAATTCTATATGCAGAGCATTATGCCAAGAGCCCGGAGTGGAAAGTAAGTTTGGGGCGAGTGTTGGGAGATTGGAGTGGCTAGAGGATGAAAATTTGTGGTCATTGATAGGTTCAGATGGGCAAAATCTTGGCCAGTTTAAGGGAGTGGTTGCAACAGACAAAAACCTAGTTTCGCCCAGGTTTACAAGTGTTACAGGAAGACAGCCACCTCTTGATTTAAATTTAGTTCCAGAGTTGGCGGTTAAGTTAAATGCTATTCCTGTCCGTCCATGCTTTGCTCTCATGATAGCATTTTCAGAGCCTGTGTCATCGATACCCTTCAAAGGTTTCTCAATAAAAAGTTCTGAAGTCTTAAGCTGGGCTCATTGTGACAGCAGCAAGCCTGGCCGTTCTACCTCCAGCGAGCGATGGGTTTTGCATTCAACAATGGAGTATGCTCAGAGTGTAATTGCTCAAACTGGACTTCAGAAACTTTCGAATGCAACATTGACAAAAGTGGCTGAAGAACTATTCCAAGAATTTCAAAGCATGGGACTTAATATTCCACACGCCTTTTTCAAGAAAGCTCATAGATGGGGAAGTGCTTTTCCAGCCGCAAGCGTAGCCGGAGACGAGAAATGCCTTTGGGATAAGAACAAGAGGCTTGCTATCTGTGGAGATTTCTGTGTTAGTCCTAATGTTGAAGGTGCTATAGCTAGCGGAATAGCTGCTGCGTCGAAACTCACAGAGATCCTCAGCTGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

369

Amino Acids

40.02

Weight (kDa)

7.52

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 5 - 43 3.8e-06 FAD dependent oxidoreductase
Pyr_redox_2 PF07992 5 - 41 9.4e-06 Pyridine nucleotide-disulphide oxidoreductase
FAD_oxidored PF12831 6 - 45 8.5e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 8 - 77 6e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 13 - 77 1.8e-06 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 20, 117, 121, 599, 954, 1059
AclWI GGATC 1 cut(s) 1084
AcoI YGGCCR 3 cut(s) 118, 490, 745
AcsI RAATTY 6 cut(s) 269, 299, 361, 451, 580, 878
AcuI CTGAAG 3 cut(s) 726, 809, 882
AfiI CCNNNNNNNGG 3 cut(s) 316, 499, 683
AflII CTTAAG 1 cut(s) 712
AgsI TTSAA 8 cut(s) 149, 193, 314, 682, 783, 884, 919, 1043
AjnI CCWGG 3 cut(s) 352, 536, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 6 cut(s) 163, 717, 926, 1055, 1067, 1098
AluI AGCT 6 cut(s) 163, 717, 926, 1055, 1067, 1098
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 132, 963
AlwI GGATC 1 cut(s) 1084
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 4 cut(s) 109, 118, 490, 745
ApeKI GCWGC 4 cut(s) 735, 1067, 1070, 1098
ApoI RAATTY 6 cut(s) 269, 299, 361, 451, 580, 878
AseI ATTAAT 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 2 cut(s) 114, 393
AsuII TTCGAA 1 cut(s) 836
AsuNHI GCTAGC 1 cut(s) 1055
BalI TGGCCA 1 cut(s) 492
BanII GRGCYC 2 cut(s) 393, 724
BbsI GAAGAC 1 cut(s) 565
Bbv12I GWGCWC 1 cut(s) 184
BbvCI CCTCAGC 1 cut(s) 1094
BbvI GCAGC 4 cut(s) 747, 1054, 1057, 1085
BccI CCATC 3 cut(s) 470, 762, 927
BceAI ACGGC 1 cut(s) 732
BciT130I CCWGG 3 cut(s) 354, 538, 744
BcnI CCSGG 2 cut(s) 114, 393
BcoDI GTCTC 2 cut(s) 132, 963
BfaI CTAG 3 cut(s) 440, 527, 1056
BfmI CTRYAG 1 cut(s) 1050
BfrI CTTAAG 1 cut(s) 712
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 7 cut(s) 118, 121, 736, 954, 1068, 1071, 1099
BlsI GCNGC 7 cut(s) 119, 122, 737, 955, 1069, 1072, 1100
Bme1390I CCNGG 5 cut(s) 114, 354, 393, 538, 744
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 5 cut(s) 114, 354, 393, 538, 744
BmsI GCATC 1 cut(s) 65
BmtI GCTAGC 1 cut(s) 1059
BpiI GAAGAC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 742
Bpu10I CCTNAGC 1 cut(s) 1094
Bpu14I TTCGAA 1 cut(s) 836
BpuMI CCSGG 2 cut(s) 114, 393
Bsa29I ATCGAT 1 cut(s) 671
BsaJI CCNNGG 4 cut(s) 112, 175, 207, 536
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 152, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 499, 683
Bse1I ACTGG 2 cut(s) 493, 823
BseBI CCWGG 3 cut(s) 354, 538, 744
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 4 cut(s) 112, 175, 207, 536
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 3 cut(s) 316, 499, 683
BseMII CTCAG 2 cut(s) 143, 812
BseNI ACTGG 2 cut(s) 493, 823
BseX3I CGGCCG 1 cut(s) 118
BseXI GCAGC 4 cut(s) 747, 1054, 1057, 1085
BseYI CCCAGC 1 cut(s) 717
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 4 cut(s) 111, 120, 492, 747
BshVI ATCGAT 1 cut(s) 671
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 3 cut(s) 114, 393, 966
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 3 cut(s) 316, 499, 683
BsmAI GTCTC 2 cut(s) 132, 963
BsmBI CGTCTC 1 cut(s) 963
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 2 cut(s) 778, 844
BsnI GGCC 4 cut(s) 111, 120, 492, 747
Bsp119I TTCGAA 1 cut(s) 836
Bsp1286I GDGCHC 3 cut(s) 184, 393, 724
Bsp143I GATC 2 cut(s) 283, 1089
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 6 cut(s) 20, 117, 121, 599, 954, 1059
BspANI GGCC 4 cut(s) 111, 120, 492, 747
BspCNI CTCAG 3 cut(s) 142, 811, 1107
BspDI ATCGAT 1 cut(s) 671
BspHI TCATGA 1 cut(s) 642
BspLI GGNNCC 1 cut(s) 112
BspOI GCTAGC 1 cut(s) 1059
BspPI GGATC 1 cut(s) 1084
BspT104I TTCGAA 1 cut(s) 836
BspTI CTTAAG 1 cut(s) 712
BsrI ACTGG 2 cut(s) 493, 823
BssECI CCNNGG 4 cut(s) 112, 175, 207, 536
BssMI GATC 2 cut(s) 283, 1089
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 3 cut(s) 354, 538, 744
Bst6I CTCTTC 1 cut(s) 130
BstAFI CTTAAG 1 cut(s) 712
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 836
BstC8I GCNNGC 6 cut(s) 256, 740, 764, 958, 1005, 1057
BstDEI CTNAG 3 cut(s) 129, 798, 1094
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 2 cut(s) 286, 1092
BstMAI GTCTC 2 cut(s) 132, 963
BstMBI GATC 2 cut(s) 283, 1089
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 5 cut(s) 53, 117, 744, 950, 962
BstNI CCWGG 3 cut(s) 354, 538, 744
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 5 cut(s) 112, 352, 391, 536, 742
BstSFI CTRYAG 1 cut(s) 1050
BstV1I GCAGC 4 cut(s) 747, 1054, 1057, 1085
BstV2I GAAGAC 1 cut(s) 565
BstX2I RGATCY 1 cut(s) 1089
BstYI RGATCY 1 cut(s) 1089
BstZI CGGCCG 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 671
BsuRI GGCC 4 cut(s) 111, 120, 492, 747
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 781
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 6 cut(s) 256, 740, 764, 958, 1005, 1057
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 642
CciNI GCGGCCGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 671
CseI GACGC 1 cut(s) 1062
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 6 cut(s) 124, 176, 358, 630, 643, 889
DdeI CTNAG 3 cut(s) 129, 798, 1094
DpnI GATC 2 cut(s) 285, 1091
DpnII GATC 2 cut(s) 283, 1089
DraI TTTAAA 1 cut(s) 579
EaeI YGGCCR 3 cut(s) 118, 490, 745
EagI CGGCCG 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 2 cut(s) 393, 724
Eco52I CGGCCG 1 cut(s) 118
Eco57I CTGAAG 3 cut(s) 726, 809, 882
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 352, 536, 742
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 2 cut(s) 393, 724
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 963
FaeI CATG 6 cut(s) 127, 179, 361, 633, 646, 892
FaqI GGGAC 1 cut(s) 906
FatI CATG 6 cut(s) 123, 175, 357, 629, 642, 888
Fnu4HI GCNGC 7 cut(s) 118, 121, 736, 954, 1068, 1071, 1099
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 2 cut(s) 393, 724
Fsp4HI GCNGC 7 cut(s) 118, 121, 736, 954, 1068, 1071, 1099
FspBI CTAG 3 cut(s) 440, 527, 1056
GluI GCNGC 7 cut(s) 118, 121, 736, 954, 1068, 1071, 1099
GsaI CCCAGC 1 cut(s) 721
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 4 cut(s) 111, 120, 492, 747
HapII CCGG 3 cut(s) 114, 393, 966
HgaI GACGC 1 cut(s) 1062
Hin1II CATG 6 cut(s) 127, 179, 361, 633, 646, 892
HinfI GANTC 2 cut(s) 98, 236
HpaII CCGG 3 cut(s) 114, 393, 966
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 6 cut(s) 132, 475, 658, 706, 801, 828
Hpy188III TCNNGA 5 cut(s) 227, 572, 590, 643, 919
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 1 cut(s) 1078
HpyAV CCTTC 4 cut(s) 151, 314, 688, 1037
HpyCH4V TGCA 6 cut(s) 41, 56, 372, 512, 778, 842
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 117, 744, 950, 962
HpyF3I CTNAG 3 cut(s) 129, 798, 1094
Hsp92II CATG 6 cut(s) 127, 179, 361, 633, 646, 892
Kzo9I GATC 2 cut(s) 283, 1089
LmnI GCTCC 3 cut(s) 44, 160, 187
Lsp1109I GCAGC 4 cut(s) 747, 1054, 1057, 1085
LweI GCATC 1 cut(s) 65
MaeI CTAG 3 cut(s) 440, 527, 1056
MaeIII GTNAC 3 cut(s) 85, 550, 728
MalI GATC 2 cut(s) 285, 1091
MboI GATC 2 cut(s) 283, 1089
MboII GAAGA 4 cut(s) 147, 161, 570, 875
MflI RGATCY 1 cut(s) 1089
MhlI GDGCHC 3 cut(s) 184, 393, 724
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 8 cut(s) 36, 269, 299, 361, 451, 580, 807, 878
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 7 cut(s) 101, 202, 436, 579, 766, 993, 1103
Mox20I TGGCCA 1 cut(s) 492
MscI TGGCCA 1 cut(s) 492
MseI TTAA 7 cut(s) 326, 498, 578, 603, 608, 713, 897
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 1098
MspCI CTTAAG 1 cut(s) 712
MspI CCGG 3 cut(s) 114, 393, 966
MspR9I CCNGG 5 cut(s) 114, 354, 393, 538, 744
Mva1269I GAATGC 2 cut(s) 778, 844
MvaI CCWGG 3 cut(s) 354, 538, 744
MwoI GCNNNNNNNGC 5 cut(s) 53, 117, 744, 950, 962
NciI CCSGG 2 cut(s) 114, 393
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 283, 1089
NheI GCTAGC 1 cut(s) 1055
NlaIII CATG 6 cut(s) 127, 179, 361, 633, 646, 892
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 728
NotI GCGGCCGC 1 cut(s) 118
NspI RCATGY 1 cut(s) 127
NspV TTCGAA 1 cut(s) 836
PagI TCATGA 1 cut(s) 642
PctI GAATGC 2 cut(s) 778, 844
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 7 cut(s) 119, 122, 737, 955, 1069, 1072, 1100
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 3 cut(s) 352, 536, 742
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 3 cut(s) 352, 536, 742
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 1089
PvuII CAGCTG 1 cut(s) 1098
SaqAI TTAA 7 cut(s) 326, 498, 578, 603, 608, 713, 897
SatI GCNGC 7 cut(s) 118, 121, 736, 954, 1068, 1071, 1099
Sau3AI GATC 2 cut(s) 283, 1089
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 5 cut(s) 114, 354, 393, 538, 744
SduI GDGCHC 3 cut(s) 184, 393, 724
SfaNI GCATC 1 cut(s) 65
SfcI CTRYAG 1 cut(s) 1050
SfiI GGCCNNNNNGGCC 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 836
SmiI ATTTAAAT 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 712
SmoI CTYRAG 1 cut(s) 712
Sse9I AATT 8 cut(s) 36, 269, 299, 361, 451, 580, 807, 878
SsiI CCGC 6 cut(s) 20, 117, 121, 599, 954, 1059
SspI AATATT 1 cut(s) 901
SspMI CTAG 3 cut(s) 440, 527, 1056
StyD4I CCNGG 5 cut(s) 112, 352, 391, 536, 742
StyI CCWWGG 1 cut(s) 175
SwaI ATTTAAAT 1 cut(s) 579
TaqI TCGA 4 cut(s) 96, 671, 836, 1076
TaqII GACCGA 1 cut(s) 206
TasI AATT 8 cut(s) 36, 269, 299, 361, 451, 580, 807, 878
TauI GCSGC 3 cut(s) 120, 123, 956
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 7 cut(s) 326, 498, 578, 603, 608, 713, 897
Tru9I TTAA 7 cut(s) 326, 498, 578, 603, 608, 713, 897
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 4 cut(s) 735, 1067, 1070, 1098
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 3 cut(s) 17, 374, 462
TspGWI ACGGA 1 cut(s) 614
TspRI CASTG 1 cut(s) 54
Vha464I CTTAAG 1 cut(s) 712
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 6 cut(s) 269, 299, 361, 451, 580, 878
XceI RCATGY 1 cut(s) 127
XspI CTAG 3 cut(s) 440, 527, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.