Rh5DG189700

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008387
Physical Location & Seq
Reverse (-)
21046673 .. 21051264
4592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG189700.1

Sequence Viewer

Length: 702 bp
ATGAAAGGCACTAAGGTTGCAGTGGTGGGAAGTGGAATTTCAGGAGCTGTGTGCGCATCAAGTCTTGCCAGAAATGGACTCTCTGTGACACTGTTCGAGTCTGCTAGAGGCCCAGGTGGCCGCATGTCTCAAAGAAGAGAAAATGTTGAAGACGGGAAGGAGCTATTGTTTGACCATGGTGCTCCGTTTTTCACTGCCAACAACACCGAGGTTCTGAGTCTTGTCCATGAGTGGGAATCGAAGGGTCTGGCAGCTATCTGGAAAGAGAAATTTGGTTCTTTTGATCGCACGTCGAACAAATTTGTTGACCTAGAACAGGAAGGATTAATAAGTAAGAGATATCTGGGTATCCCAGGGATGAATTCCGTGTGCAGAGCATTATGCCGTGAGCCTGGTGTTGAAAGTAAGTTTGGGACAAGTGTTGGAAGATTGGAGTGGCTAGAGGATCAAAATTTGTGGTCATTGATTGGTTTGGATGGACAAAATCTTGGTCAGTTTAAGGGAGTGGTTGCAACAGACAAAAACATAGTTTCACCCAGGTTTACAAGTGTTACAGGAAAACCGCTACCTCTTGATTTGAAATTATTTCCAGAGTTAGCAGTTAAGTTGAATGACATTCCTGTTCGTCCGTGCTTTGCTCTAATGATAGCCTTTGCAGAACCTCTGACATCGGTGAGATACTTATTATGCAGTCTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

233

Amino Acids

25.42

Weight (kDa)

8.59

Isoelectric Point (pI)

24.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 5 - 43 8.4e-07 FAD dependent oxidoreductase
FAD_oxidored PF12831 6 - 52 1.6e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 8 - 77 1.2e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 13 - 77 6.8e-08 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AciI CCGC 2 cut(s) 121, 563
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 5 cut(s) 36, 269, 299, 361, 451
AfiI CCNNNNNNNGG 2 cut(s) 232, 316
AgsI TTSAA 4 cut(s) 149, 401, 580, 610
AjiI CACGTC 1 cut(s) 291
AjnI CCWGG 4 cut(s) 112, 352, 391, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 3 cut(s) 47, 163, 254
AluI AGCT 3 cut(s) 47, 163, 254
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 132, 698
AlwI GGATC 1 cut(s) 453
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 2 cut(s) 109, 118
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 5 cut(s) 36, 269, 299, 361, 451
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AseI ATTAAT 1 cut(s) 326
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 525, 685
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 263
BccI CCATC 1 cut(s) 470
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 4 cut(s) 114, 354, 393, 538
BciVI GTATCC 1 cut(s) 359
BcoDI GTCTC 2 cut(s) 132, 698
BfaI CTAG 3 cut(s) 105, 311, 440
BfuI GTATCC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 2 cut(s) 121, 252
BlsI GCNGC 2 cut(s) 122, 253
Bme1390I CCNGG 4 cut(s) 114, 354, 393, 538
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 110
BmrFI CCNGG 4 cut(s) 114, 354, 393, 538
BmsI GCATC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 156
BsaJI CCNNGG 6 cut(s) 112, 175, 207, 352, 353, 536
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 316
BseBI CCWGG 4 cut(s) 114, 354, 393, 538
BseDI CCNNGG 6 cut(s) 112, 175, 207, 352, 353, 536
BseGI GGATG 2 cut(s) 363, 481
BseLI CCNNNNNNNGG 2 cut(s) 232, 316
BseMII CTCAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 263
BsgI GTGCAG 1 cut(s) 391
BshFI GGCC 2 cut(s) 111, 120
BsiHKAI GWGCWC 1 cut(s) 184
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 232, 316
BsmAI GTCTC 2 cut(s) 132, 698
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 2 cut(s) 111, 120
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 2 cut(s) 283, 445
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 2 cut(s) 121, 563
BspANI GGCC 2 cut(s) 111, 120
BspCNI CTCAG 1 cut(s) 207
BspPI GGATC 1 cut(s) 453
BssECI CCNNGG 6 cut(s) 112, 175, 207, 352, 353, 536
BssMI GATC 2 cut(s) 283, 445
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 4 cut(s) 114, 354, 393, 538
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 12, 215
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 2 cut(s) 363, 481
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 286, 448
BstMAI GTCTC 2 cut(s) 132, 698
BstMBI GATC 2 cut(s) 283, 445
BstMWI GCNNNNNNNGC 2 cut(s) 53, 117
BstNI CCWGG 4 cut(s) 114, 354, 393, 538
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 4 cut(s) 112, 352, 391, 536
BstV1I GCAGC 1 cut(s) 263
BstV2I GAAGAC 1 cut(s) 156
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 2 cut(s) 111, 120
BtgI CCRYGG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 2 cut(s) 363, 481
BtsI GCAGTG 2 cut(s) 27, 192
BtsIMutI CAGTG 3 cut(s) 27, 89, 192
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 110
CspCI CAANNNNNGTGG 2 cut(s) 437, 472
CviAII CATG 3 cut(s) 124, 176, 227
CviJI RGCY 8 cut(s) 47, 111, 120, 163, 254, 391, 439, 650
CviKI_1 RGCY 8 cut(s) 47, 111, 120, 163, 254, 391, 439, 650
DdeI CTNAG 2 cut(s) 12, 215
DpnI GATC 2 cut(s) 285, 447
DpnII GATC 2 cut(s) 283, 445
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 175
Eco32I GATATC 1 cut(s) 341
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 4 cut(s) 112, 352, 391, 536
EcoRV GATATC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 3 cut(s) 127, 179, 230
FaiI YATR 6 cut(s) 125, 177, 228, 382, 527, 688
FaqI GGGAC 1 cut(s) 427
FatI CATG 3 cut(s) 123, 175, 226
Fnu4HI GCNGC 2 cut(s) 121, 252
FokI GGATG 2 cut(s) 370, 488
Fsp4HI GCNGC 2 cut(s) 121, 252
FspAI RTGCGCAY 1 cut(s) 55
FspBI CTAG 3 cut(s) 105, 311, 440
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 121, 252
HaeIII GGCC 2 cut(s) 111, 120
HhaI GCGC 1 cut(s) 56
Hin1II CATG 3 cut(s) 127, 179, 230
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 4 cut(s) 78, 98, 217, 236
HphI GGTGA 2 cut(s) 525, 685
Hpy166II GTNNAC 2 cut(s) 307, 543
Hpy188I TCNGA 2 cut(s) 216, 666
Hpy188III TCNNGA 4 cut(s) 42, 259, 572, 590
Hpy8I GTNNAC 2 cut(s) 307, 543
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 151, 235, 314
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 5 cut(s) 20, 372, 512, 656, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 117
HpyF3I CTNAG 2 cut(s) 12, 215
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 127, 179, 230
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 283, 445
LmnI GCTCC 3 cut(s) 44, 160, 187
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 1 cut(s) 65
MaeI CTAG 3 cut(s) 105, 311, 440
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 85, 550
MalI GATC 2 cut(s) 285, 447
MboI GATC 2 cut(s) 283, 445
MboII GAAGA 3 cut(s) 147, 161, 438
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 6 cut(s) 36, 269, 299, 361, 451, 581
MlyI GAGTC 3 cut(s) 72, 107, 226
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 5 cut(s) 101, 202, 436, 579, 672
MseI TTAA 3 cut(s) 326, 498, 603
MspR9I CCNGG 4 cut(s) 114, 354, 393, 538
MvaI CCWGG 4 cut(s) 114, 354, 393, 538
MwoI GCNNNNNNNGC 2 cut(s) 53, 117
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 283, 445
NlaIII CATG 3 cut(s) 127, 179, 230
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 55
NspI RCATGY 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 353
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 122, 253
PleI GAGTC 3 cut(s) 72, 106, 225
PpsI GAGTC 3 cut(s) 72, 106, 225
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 4 cut(s) 112, 352, 391, 536
PspGI CCWGG 4 cut(s) 112, 352, 391, 536
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 47
SaqAI TTAA 3 cut(s) 326, 498, 603
SatI GCNGC 2 cut(s) 121, 252
Sau3AI GATC 2 cut(s) 283, 445
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 3 cut(s) 72, 107, 226
ScrFI CCNGG 4 cut(s) 114, 354, 393, 538
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 1 cut(s) 65
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sse9I AATT 6 cut(s) 36, 269, 299, 361, 451, 581
SsiI CCGC 2 cut(s) 121, 563
SspMI CTAG 3 cut(s) 105, 311, 440
StyD4I CCNGG 4 cut(s) 112, 352, 391, 536
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 96, 239, 293
TasI AATT 6 cut(s) 36, 269, 299, 361, 451, 581
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 3 cut(s) 326, 498, 603
Tru9I TTAA 3 cut(s) 326, 498, 603
TscAI CASTG 3 cut(s) 27, 96, 199
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 17, 374
TspGWI ACGGA 3 cut(s) 174, 355, 618
TspRI CASTG 3 cut(s) 27, 96, 199
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 5 cut(s) 36, 269, 299, 361, 451
XceI RCATGY 1 cut(s) 127
XspI CTAG 3 cut(s) 105, 311, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.