Rh5BG187900

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008387
Physical Location & Seq
Reverse (-)
20631406 .. 20635358
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG187900.1

Sequence Viewer

Length: 969 bp
ATGAAAGGCACTAAGGTTGCAGTGGTGGGAAGTGGCATTTCAGGAGCTGTGTGCGCATCAAGTCTTGCCAGAAATGGACTCTCTGTGACACTGTTCGAGTCTGCTAGAGGCCCAGGTGGCCGCATGTCTCAAAGAAGAGAAAATGTTGAAGACGGGAAGGAGCTATTGTTTGACCATGGTGCTCCGTTTTTCACTGCCAACAACACCGAGGTTCTGAGTCTTGTCCATGAGTGGGAATCGAAGGGTCTGGCAGCTATCTGGAAAGAGAAATTTGGATCTTTTGATCGCACGTCAAACAAATTTGTTGACCTAGAACAGGAAGGATTAATAAGTAAGAGATATCTGGGTATCCCAGGGATGAATTCTGTGTGCAGAGCATTATGCCGTGAGCCTGGTGTTGAAAGTAAGTTTGGGACAAGTGTTGGAAGATTGGAGTGGCTAGAGGATCAAAATTTGTGGTCATTGATTGGTTTGGATGGACAAAATCTTGGTCAGTTTAAGGGAGTGGTTGCAACAGACAAAAACATAGTTTCACCCAGGTTTACAAGTGTTACAGGAAAACCACTACCTCTTGATTTGAAATTATTTCCAGAGTTAGCAGTTAAGTTGAATGACATTCCTGTTCGTCCGTGCTTTGCTCTAATGATAGCCTTTGCAGAACCTCTGACATCGATACCCTTTAAAGGCTTCTCAATAAAAAATTCTGAAGTTTTAAGCTGGGCTCATTGTGATAGCAGCAAGCCTGGCCGTTCTACCTCTAGTGAGCGATGGGTTCTGCATTCTACAATGGAGTATGCTCAGAGAATAATTGCTCAAACTGGACTTCAGAAGCTTTCAGATGCAACATTGACCAAAGTGGCTGAAGAACTATTCCAAGAATTACAAAGCCTGGGACTTAATATTTCACAGCCCTTTTTCAAGAAAGCTCATAGATGGTCAGTATATATTACTTTAGACCCAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

322

Amino Acids

35.52

Weight (kDa)

8.73

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 5 - 43 1.3e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 6 - 52 3.7e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 8 - 77 2.2e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 13 - 77 1.2e-07 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 283, 453
AcoI YGGCCR 2 cut(s) 118, 745
AcsI RAATTY 5 cut(s) 269, 299, 361, 451, 700
AcuI CTGAAG 3 cut(s) 726, 809, 882
AfiI CCNNNNNNNGG 3 cut(s) 232, 316, 683
AgsI TTSAA 5 cut(s) 149, 401, 580, 610, 919
AjiI CACGTC 1 cut(s) 291
AjnI CCWGG 6 cut(s) 112, 352, 391, 536, 742, 888
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 6 cut(s) 47, 163, 254, 717, 832, 926
AluI AGCT 6 cut(s) 47, 163, 254, 717, 832, 926
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 2 cut(s) 283, 453
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 3 cut(s) 109, 118, 745
ApeKI GCWGC 2 cut(s) 251, 735
ApoI RAATTY 5 cut(s) 269, 299, 361, 451, 700
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AseI ATTAAT 1 cut(s) 326
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 525
BanII GRGCYC 1 cut(s) 724
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 2 cut(s) 263, 747
BccI CCATC 3 cut(s) 470, 762, 927
BceAI ACGGC 2 cut(s) 369, 732
BciT130I CCWGG 6 cut(s) 114, 354, 393, 538, 744, 890
BciVI GTATCC 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 4 cut(s) 105, 311, 440, 759
BfuI GTATCC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 3 cut(s) 121, 252, 736
BlsI GCNGC 3 cut(s) 122, 253, 737
Bme1390I CCNGG 6 cut(s) 114, 354, 393, 538, 744, 890
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 110
BmrFI CCNGG 6 cut(s) 114, 354, 393, 538, 744, 890
BmsI GCATC 2 cut(s) 65, 829
BpiI GAAGAC 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 671
BsaJI CCNNGG 7 cut(s) 112, 175, 207, 352, 353, 536, 889
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 316, 683
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 6 cut(s) 114, 354, 393, 538, 744, 890
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 7 cut(s) 112, 175, 207, 352, 353, 536, 889
BseGI GGATG 2 cut(s) 363, 481
BseLI CCNNNNNNNGG 3 cut(s) 232, 316, 683
BseMII CTCAG 2 cut(s) 206, 812
BseNI ACTGG 1 cut(s) 823
BseXI GCAGC 2 cut(s) 263, 747
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 391
BshFI GGCC 3 cut(s) 111, 120, 747
BshVI ATCGAT 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 184
BslFI GGGAC 2 cut(s) 427, 906
BslI CCNNNNNNNGG 3 cut(s) 232, 316, 683
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 2 cut(s) 427, 906
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 3 cut(s) 111, 120, 747
Bsp1286I GDGCHC 2 cut(s) 184, 724
Bsp143I GATC 3 cut(s) 275, 283, 445
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 121
BspANI GGCC 3 cut(s) 111, 120, 747
BspCNI CTCAG 2 cut(s) 207, 811
BspDI ATCGAT 1 cut(s) 671
BspPI GGATC 2 cut(s) 283, 453
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 7 cut(s) 112, 175, 207, 352, 353, 536, 889
BssMI GATC 3 cut(s) 275, 283, 445
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 6 cut(s) 114, 354, 393, 538, 744, 890
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 3 cut(s) 12, 215, 798
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 2 cut(s) 363, 481
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 3 cut(s) 278, 286, 448
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 3 cut(s) 275, 283, 445
BstMWI GCNNNNNNNGC 3 cut(s) 53, 117, 744
BstNI CCWGG 6 cut(s) 114, 354, 393, 538, 744, 890
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 6 cut(s) 112, 352, 391, 536, 742, 888
BstV1I GCAGC 2 cut(s) 263, 747
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 671
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 111, 120, 747
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 781
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 2 cut(s) 363, 481
BtsI GCAGTG 2 cut(s) 27, 192
BtsIMutI CAGTG 3 cut(s) 27, 89, 192
Cac8I GCNNGC 1 cut(s) 740
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 671
CspCI CAANNNNNGTGG 2 cut(s) 437, 472
CviAII CATG 3 cut(s) 124, 176, 227
DdeI CTNAG 3 cut(s) 12, 215, 798
DpnI GATC 3 cut(s) 277, 285, 447
DpnII GATC 3 cut(s) 275, 283, 445
DraI TTTAAA 1 cut(s) 682
EaeI YGGCCR 2 cut(s) 118, 745
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 724
Eco32I GATATC 1 cut(s) 341
Eco57I CTGAAG 3 cut(s) 726, 809, 882
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 6 cut(s) 112, 352, 391, 536, 742, 888
EcoRV GATATC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 3 cut(s) 127, 179, 230
FaiI YATR 9 cut(s) 125, 177, 228, 382, 527, 795, 930, 943, 945
FaqI GGGAC 2 cut(s) 427, 906
FatI CATG 3 cut(s) 123, 175, 226
Fnu4HI GCNGC 3 cut(s) 121, 252, 736
FokI GGATG 2 cut(s) 370, 488
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 3 cut(s) 121, 252, 736
FspAI RTGCGCAY 1 cut(s) 55
FspBI CTAG 4 cut(s) 105, 311, 440, 759
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 3 cut(s) 121, 252, 736
GsaI CCCAGC 1 cut(s) 721
HaeIII GGCC 3 cut(s) 111, 120, 747
HhaI GCGC 1 cut(s) 56
Hin1II CATG 3 cut(s) 127, 179, 230
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 4 cut(s) 78, 98, 217, 236
HphI GGTGA 1 cut(s) 525
Hpy166II GTNNAC 2 cut(s) 307, 543
Hpy188I TCNGA 6 cut(s) 216, 666, 706, 801, 828, 838
Hpy188III TCNNGA 5 cut(s) 42, 259, 572, 590, 919
Hpy8I GTNNAC 2 cut(s) 307, 543
HpyAV CCTTC 3 cut(s) 151, 235, 314
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 6 cut(s) 20, 372, 512, 656, 778, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 117, 744
HpyF3I CTNAG 3 cut(s) 12, 215, 798
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 127, 179, 230
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 3 cut(s) 275, 283, 445
LmnI GCTCC 3 cut(s) 44, 160, 187
Lsp1109I GCAGC 2 cut(s) 263, 747
LweI GCATC 2 cut(s) 65, 829
MaeI CTAG 4 cut(s) 105, 311, 440, 759
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 85, 550
MalI GATC 3 cut(s) 277, 285, 447
MboI GATC 3 cut(s) 275, 283, 445
MboII GAAGA 4 cut(s) 147, 161, 438, 875
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 184, 724
MluCI AATT 8 cut(s) 269, 299, 361, 451, 581, 700, 807, 878
MlyI GAGTC 3 cut(s) 72, 107, 226
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 6 cut(s) 101, 202, 436, 579, 672, 766
MseI TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
MspR9I CCNGG 6 cut(s) 114, 354, 393, 538, 744, 890
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 6 cut(s) 114, 354, 393, 538, 744, 890
MwoI GCNNNNNNNGC 3 cut(s) 53, 117, 744
NcoI CCATGG 1 cut(s) 175
NdeII GATC 3 cut(s) 275, 283, 445
NlaIII CATG 3 cut(s) 127, 179, 230
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 55
NspI RCATGY 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 353
PctI GAATGC 1 cut(s) 778
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 122, 253, 737
PleI GAGTC 3 cut(s) 72, 106, 225
PpsI GAGTC 3 cut(s) 72, 106, 225
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 6 cut(s) 112, 352, 391, 536, 742, 888
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 6 cut(s) 112, 352, 391, 536, 742, 888
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 47
PsuI RGATCY 1 cut(s) 275
SaqAI TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
SatI GCNGC 3 cut(s) 121, 252, 736
Sau3AI GATC 3 cut(s) 275, 283, 445
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 3 cut(s) 72, 107, 226
ScrFI CCNGG 6 cut(s) 114, 354, 393, 538, 744, 890
SduI GDGCHC 2 cut(s) 184, 724
SfaNI GCATC 2 cut(s) 65, 829
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sse9I AATT 8 cut(s) 269, 299, 361, 451, 581, 700, 807, 878
SsiI CCGC 1 cut(s) 121
SspI AATATT 1 cut(s) 901
SspMI CTAG 4 cut(s) 105, 311, 440, 759
StyD4I CCNGG 6 cut(s) 112, 352, 391, 536, 742, 888
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 96, 239, 671
TasI AATT 8 cut(s) 269, 299, 361, 451, 581, 700, 807, 878
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
Tru9I TTAA 6 cut(s) 326, 498, 603, 681, 713, 897
TscAI CASTG 3 cut(s) 27, 96, 199
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 251, 735
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 17, 374
TspGWI ACGGA 2 cut(s) 174, 618
TspRI CASTG 3 cut(s) 27, 96, 199
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 5 cut(s) 269, 299, 361, 451, 700
XceI RCATGY 1 cut(s) 127
XspI CTAG 4 cut(s) 105, 311, 440, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.