Rw5G017330

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008387
Physical Location & Seq
Reverse (-)
22093802 .. 22098054
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017330.1

Sequence Viewer

Length: 1107 bp
ATGAAAGGCACTAAGGTTGCAGTGGTGGGAAGTGGAATTTCAGGAGCTGTGTGCGCATCAAGTCTTGCCAGAAATGGACTCTCTGTGACACTGTTCGAGTCTGCTAGAGGCCCAGGTGGCCGCATGTCTCAAAGAAGAGAAAATGTTGAAGACGGGAAGGAGCTATTGTTTGACCATGGTGCTCCGTTTTTCACTGCCAACAACACCGAGGTTCTGAGTCTTGTCCATGAGTGGGAATCAAAGGGTCTGGCAGCTATCTGGAAAGAGAAATTTGGTTCTTTTGATCGCACGTCGAACAAATTTGTTGACCTAGAACAGGAAGGATTAATAAGTAAGAGATATCTGGGTATCCCAGGGATGAATTCTGTGTGCAGAGCATTATGCCGTGAGCCTGGTGTTGAAAGTAAGTTTGGGACAAGTGTTGGAAGATTGGAGTGGCTAGAGGATCAAAATTTGTGGTCATTGATTGGTTTGGATGGACAAAATCTTGGTCAGTTTAAGGGAGTGGTTGCAACAGACAAAAACATAGTTTCACCCAGGTTTACAAGTGTTACAGGAAAACCACTACCTCTTGATTTGAAATTATTTCCAGAGTTAGCAGTTAAGTTGAATGACATTCCTGTTCGTCCGTGCTTTGCTTTAATGATAGCCTTTGCAGAACCTCTGACATCGATACCCTTTAAAGGCTTCTCAATAAAAAATTCTGAAGTTTTAAGCTGGGCTCATTGTGATAGCAGCAAGCCTGGCCGTTCTACCTCTAGTGAGCGATGGGTTCTGCATTCTACAATGGAGTATGCTCAGAGAATAATTGCTCAAACTGGACTTCAGAAGCTTTCAGATGCAACATTGACCAAAGTGGCTGAAGAACTATTCCAAGAATTACAAAGCCTGGGACTTAATATTTCACAGCCCTTTTTCAAGAAAGCTCATAGATGGGGAAGTGCTTTTCCAGCTACAAGCATAGCCAGAGAGGAGAAATGTCTTTGGGATGGGAACAAGAGGTTTGCCATCTGCGGTGATTTCTGTGTTAGTCCAGATGTTGAAGGTGCTATAGTTAGTGGCCTGGCCGCGGCTTCCAAACTTTCAGAGGTCTTCAGCTGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

368

Amino Acids

40.16

Weight (kDa)

8.03

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 5 - 43 1.6e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 6 - 52 5e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 8 - 77 2.7e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 13 - 77 1.5e-07 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 55
AccII CGCG 1 cut(s) 1070
AciI CCGC 4 cut(s) 121, 1014, 1068, 1070
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 3 cut(s) 118, 745, 1065
AcsI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
AcuI CTGAAG 4 cut(s) 726, 809, 882, 1078
AfiI CCNNNNNNNGG 4 cut(s) 232, 316, 683, 1069
AgsI TTSAA 6 cut(s) 149, 401, 580, 610, 919, 1043
AjiI CACGTC 1 cut(s) 291
AjnI CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 393, 425
AluBI AGCT 8 cut(s) 47, 163, 254, 717, 832, 926, 953, 1098
AluI AGCT 8 cut(s) 47, 163, 254, 717, 832, 926, 953, 1098
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 453
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 5 cut(s) 109, 118, 745, 1060, 1065
ApeKI GCWGC 3 cut(s) 251, 735, 1098
ApoI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AseI ATTAAT 1 cut(s) 326
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 525, 1028
BanII GRGCYC 1 cut(s) 724
BbsI GAAGAC 2 cut(s) 156, 1084
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 263, 747, 1085
BccI CCATC 5 cut(s) 470, 762, 927, 983, 1016
BceAI ACGGC 2 cut(s) 369, 732
BciT130I CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BciVI GTATCC 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 4 cut(s) 105, 311, 440, 759
BfmI CTRYAG 1 cut(s) 1050
BfuI GTATCC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
BlsI GCNGC 6 cut(s) 122, 253, 737, 1069, 1072, 1100
Bme1390I CCNGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 110
BmrFI CCNGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BmsI GCATC 2 cut(s) 65, 829
BpiI GAAGAC 2 cut(s) 156, 1084
Bsa29I ATCGAT 1 cut(s) 671
BsaJI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 536, 889, 1068
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 316, 683, 1069
Bse1I ACTGG 1 cut(s) 823
BseBI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 536, 889, 1068
BseGI GGATG 3 cut(s) 363, 481, 994
BseLI CCNNNNNNNGG 4 cut(s) 232, 316, 683, 1069
BseMII CTCAG 2 cut(s) 206, 812
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 3 cut(s) 263, 747, 1085
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 1070
BshFI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BshVI ATCGAT 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 184
BslFI GGGAC 2 cut(s) 427, 906
BslI CCNNNNNNNGG 4 cut(s) 232, 316, 683, 1069
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 2 cut(s) 427, 906
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
Bsp1286I GDGCHC 2 cut(s) 184, 724
Bsp143I GATC 2 cut(s) 283, 445
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 4 cut(s) 121, 1014, 1068, 1070
BspANI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BspCNI CTCAG 2 cut(s) 207, 811
BspDI ATCGAT 1 cut(s) 671
BspFNI CGCG 1 cut(s) 1070
BspPI GGATC 1 cut(s) 453
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 8 cut(s) 112, 175, 207, 352, 353, 536, 889, 1068
BssMI GATC 2 cut(s) 283, 445
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 3 cut(s) 12, 215, 798
BstDSI CCRYGG 2 cut(s) 175, 1068
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 3 cut(s) 363, 481, 994
BstFNI CGCG 1 cut(s) 1070
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 286, 448
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 283, 445
BstMWI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
BstNI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
BstSFI CTRYAG 1 cut(s) 1050
BstUI CGCG 1 cut(s) 1070
BstV1I GCAGC 3 cut(s) 263, 747, 1085
BstV2I GAAGAC 2 cut(s) 156, 1084
Bsu15I ATCGAT 1 cut(s) 671
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 5 cut(s) 111, 120, 747, 1062, 1067
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 2 cut(s) 175, 1068
BtgZI GCGATG 1 cut(s) 781
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 3 cut(s) 363, 481, 994
BtsI GCAGTG 2 cut(s) 27, 192
BtsIMutI CAGTG 3 cut(s) 27, 89, 192
Cac8I GCNNGC 1 cut(s) 740
CaiI CAGNNNCTG 1 cut(s) 47
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 110
Cfr42I CCGCGG 1 cut(s) 1071
ClaI ATCGAT 1 cut(s) 671
CspCI CAANNNNNGTGG 2 cut(s) 437, 472
CviAII CATG 3 cut(s) 124, 176, 227
DdeI CTNAG 3 cut(s) 12, 215, 798
DpnI GATC 2 cut(s) 285, 447
DpnII GATC 2 cut(s) 283, 445
DraI TTTAAA 1 cut(s) 682
EaeI YGGCCR 3 cut(s) 118, 745, 1065
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 724
Eco32I GATATC 1 cut(s) 341
Eco57I CTGAAG 4 cut(s) 726, 809, 882, 1078
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
EcoRV GATATC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 3 cut(s) 127, 179, 230
FaqI GGGAC 2 cut(s) 427, 906
FatI CATG 3 cut(s) 123, 175, 226
Fnu4HI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
FokI GGATG 3 cut(s) 370, 488, 1001
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
FspAI RTGCGCAY 1 cut(s) 55
FspBI CTAG 4 cut(s) 105, 311, 440, 759
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
GsaI CCCAGC 1 cut(s) 721
HaeIII GGCC 5 cut(s) 111, 120, 747, 1062, 1067
HhaI GCGC 1 cut(s) 56
Hin1II CATG 3 cut(s) 127, 179, 230
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 4 cut(s) 78, 98, 217, 236
HphI GGTGA 2 cut(s) 525, 1028
Hpy166II GTNNAC 2 cut(s) 307, 543
Hpy188I TCNGA 7 cut(s) 216, 666, 706, 801, 828, 838, 1087
Hpy188III TCNNGA 6 cut(s) 42, 259, 572, 590, 919, 1034
Hpy8I GTNNAC 2 cut(s) 307, 543
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 151, 314, 1037
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 6 cut(s) 20, 372, 512, 656, 778, 842
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
HpyF3I CTNAG 3 cut(s) 12, 215, 798
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 127, 179, 230
HspAI GCGC 1 cut(s) 54
KspI CCGCGG 1 cut(s) 1071
Kzo9I GATC 2 cut(s) 283, 445
LmnI GCTCC 3 cut(s) 44, 160, 187
Lsp1109I GCAGC 3 cut(s) 263, 747, 1085
LweI GCATC 2 cut(s) 65, 829
MaeI CTAG 4 cut(s) 105, 311, 440, 759
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 85, 550
MalI GATC 2 cut(s) 285, 447
MboI GATC 2 cut(s) 283, 445
MboII GAAGA 5 cut(s) 147, 161, 438, 875, 1084
MhlI GDGCHC 2 cut(s) 184, 724
MluCI AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
MlyI GAGTC 3 cut(s) 72, 107, 226
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 9 cut(s) 101, 202, 436, 579, 672, 766, 964, 993, 1081
MseI TTAA 7 cut(s) 326, 498, 603, 641, 681, 713, 897
MspA1I CMGCKG 2 cut(s) 1070, 1098
MspR9I CCNGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
MvnI CGCG 1 cut(s) 1070
MwoI GCNNNNNNNGC 4 cut(s) 53, 117, 744, 950
NcoI CCATGG 1 cut(s) 175
NdeII GATC 2 cut(s) 283, 445
NlaIII CATG 3 cut(s) 127, 179, 230
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 55
NspI RCATGY 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 353
PctI GAATGC 1 cut(s) 778
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 6 cut(s) 122, 253, 737, 1069, 1072, 1100
PleI GAGTC 3 cut(s) 72, 106, 225
PpsI GAGTC 3 cut(s) 72, 106, 225
PshBI ATTAAT 1 cut(s) 326
Psp6I CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 1098
SacII CCGCGG 1 cut(s) 1071
SaqAI TTAA 7 cut(s) 326, 498, 603, 641, 681, 713, 897
SatI GCNGC 6 cut(s) 121, 252, 736, 1068, 1071, 1099
Sau3AI GATC 2 cut(s) 283, 445
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 3 cut(s) 72, 107, 226
ScrFI CCNGG 7 cut(s) 114, 354, 393, 538, 744, 890, 1064
SduI GDGCHC 2 cut(s) 184, 724
SfaNI GCATC 2 cut(s) 65, 829
SfcI CTRYAG 1 cut(s) 1050
SfiI GGCCNNNNNGGCC 1 cut(s) 117
Sfr303I CCGCGG 1 cut(s) 1071
SgrBI CCGCGG 1 cut(s) 1071
Sse9I AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
SsiI CCGC 4 cut(s) 121, 1014, 1068, 1070
SspI AATATT 1 cut(s) 901
SspMI CTAG 4 cut(s) 105, 311, 440, 759
StyD4I CCNGG 7 cut(s) 112, 352, 391, 536, 742, 888, 1062
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 96, 293, 671
TasI AATT 9 cut(s) 36, 269, 299, 361, 451, 581, 700, 807, 878
TauI GCSGC 3 cut(s) 123, 1070, 1073
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 7 cut(s) 326, 498, 603, 641, 681, 713, 897
Tru9I TTAA 7 cut(s) 326, 498, 603, 641, 681, 713, 897
TscAI CASTG 3 cut(s) 27, 96, 199
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 3 cut(s) 251, 735, 1098
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 17, 374
TspGWI ACGGA 2 cut(s) 174, 618
TspRI CASTG 3 cut(s) 27, 96, 199
VspI ATTAAT 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 6 cut(s) 36, 269, 299, 361, 451, 700
XceI RCATGY 1 cut(s) 127
XspI CTAG 4 cut(s) 105, 311, 440, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.