MD10G1198300.v1.1

Photosystem II 10 kDa polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016135
Physical Location & Seq
Forward (+)
29623582 .. 29631938
8357 bp
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UTR
Exon/CDS
Intron
MD10G1198300.v1.1.491

Sequence Viewer

Length: 423 bp
ATGTCTGCCTCAGTTATGGCTTGTTCCGTGAGCCTAAAACCATCTCCCTTCACTGTTCAGAAGTCAGCAGTGAGAGGCCTTCCCTCTCTTTCCAGGTCTTCTGCTTCATTCAAGGTGCAAGCCAGTGGCGTCAAGAAAATCAAGACTGCCACCCCATATGGAACTGGTGGCGGCATGGAGTTGAGGAACGGTGTTGATGCCTCTGGGAGGAAGCCTACGGGAAAGGGTGTCTACCAGTTTGTAGACAAGTACGGTGCTAATGTTGATGGATACAGTCCCATCTACAACAAAGATGAGTGGGCTCCATCTGGTGATGTCTATGCTGGGGGTCTTACTGGCTTGGCCATCTGGGCAGTTACCCTCTTTGGCATTCTTGCAGGGGGTGCCCTTCTTGTCTACAACACAAGTGCTTTGTCACAGTAA

Protein Analysis

141

Amino Acids

14.43

Weight (kDa)

9.64

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbR PF04725 42 - 139 3.3e-50 Photosystem II 10 kDa polypeptide PsbR
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79040
fragaria_vesca FvH4_3g16050
malus_domestica MD05G1215200.v1.1 MD10G1198300.v1.1
prunus_persica Prupe.4G143700_v2.0.a1
pyrus_communis pycom05g19820 pycom10g17100
rosa_chinensis RchiOBHm_Chr5g0027021
rosa_laevigata RLG00000032979 RLG00000032983
rosa_roxburghii Rroxscaffold_1G00052470
rosa_rugosa Rorug05G0096600 Rorug05G0096700
rosa_samantha Rh5AG190200
rosa_wichuraiana Rw5G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 3 cut(s) 231, 243, 396
AciI CCGC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 1 cut(s) 92
AoxI GGCC 2 cut(s) 76, 342
AsuHPI GGTGA 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 388
BalI TGGCCA 1 cut(s) 344
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 90
BccI CCATC 5 cut(s) 49, 260, 287, 313, 353
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 263
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 303, 385
BmrFI CCNGG 1 cut(s) 94
BmsI GCATC 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 4 cut(s) 123, 169, 235, 340
BseBI CCWGG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 4 cut(s) 123, 169, 235, 340
BseSI GKGCMC 1 cut(s) 388
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 2 cut(s) 78, 344
BshNI GGYRCC 1 cut(s) 383
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 261
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 2 cut(s) 78, 344
Bsp1286I GDGCHC 2 cut(s) 304, 388
BspACI CCGC 1 cut(s) 171
BspANI GGCC 2 cut(s) 78, 344
BspCNI CTCAG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 303, 385
BspT107I GGYRCC 1 cut(s) 383
BsrI ACTGG 4 cut(s) 123, 169, 235, 340
BssNI GRCGYC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 5 cut(s) 55, 191, 254, 275, 420
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 10
BstENI CCTNNNNNAGG 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 350, 383
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 90
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 78, 344
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 3 cut(s) 51, 75, 130
Cac8I GCNNGC 1 cut(s) 120
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 20, 33, 78, 122, 214, 302, 339, 344
CviKI_1 RGCY 8 cut(s) 20, 33, 78, 122, 214, 302, 339, 344
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 342
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 304
FaeI CATG 1 cut(s) 178
FaiI YATR 5 cut(s) 17, 157, 159, 176, 321
FaqI GGGAC 1 cut(s) 261
FatI CATG 1 cut(s) 174
FauNDI CATATG 1 cut(s) 157
FblI GTMKAC 3 cut(s) 231, 243, 396
Fnu4HI GCNGC 1 cut(s) 172
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 172
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 2 cut(s) 78, 344
HgaI GACGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 1 cut(s) 178
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 3 cut(s) 232, 244, 397
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 2 cut(s) 133, 142
Hpy8I GTNNAC 3 cut(s) 232, 244, 397
HpyAV CCTTC 3 cut(s) 58, 89, 398
HpyCH4III ACNGT 5 cut(s) 55, 191, 254, 275, 420
HpyCH4V TGCA 2 cut(s) 118, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 383
HpyF3I CTNAG 1 cut(s) 10
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 1 cut(s) 178
LmnI GCTCC 1 cut(s) 307
LweI GCATC 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 355, 414
MboII GAAGA 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 304, 388
MlsI TGGCCA 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 344
MnlI CCTC 7 cut(s) 19, 68, 94, 177, 201, 211, 371
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 94
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 350, 383
NdeI CATATG 1 cut(s) 157
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 2 cut(s) 303, 385
NmuCI GTSAC 1 cut(s) 414
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 1 cut(s) 369
PkrI GCNGC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 303, 385
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SatI GCNGC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 2 cut(s) 304, 388
SetI ASST 2 cut(s) 98, 117
SfaNI GCATC 1 cut(s) 187
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 92
TaaI ACNGT 5 cut(s) 55, 191, 254, 275, 420
TauI GCSGC 1 cut(s) 174
TscAI CASTG 3 cut(s) 58, 75, 130
TseFI GTSAC 1 cut(s) 414
Tsp45I GTSAC 1 cut(s) 414
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 3 cut(s) 58, 75, 130
XagI CCTNNNNNAGG 1 cut(s) 205
XmiI GTMKAC 3 cut(s) 231, 243, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.