Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008093

Physical Location & Seq

Genomic Coordinates Forward (+)

36298931 .. 36300862

Gene Structure 1932 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1229600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 807 bp

ATGGATCACTCAGAACAAACACTACAGCAACAGCAGCAGCCTGTGGATGGTGCCGTAGCAAGTTCTGGCCAAATGGCATCATATGCTCCTTCGTCCTGTCAAACTGCTCCCATAGTGGCTTCTGGAACTCTTGCAGTGGCCGTCACTTCCCCATCGCAGGCCCCCACCACTTTCCCTGATCCGTCACATCAGCTTGCCTACCAGAAAATACAGCACTTTCCCCACCGCCAACAGGTGCAGCTTCAAGAGTTCTGGTCCGACCAAATGCAAGACGTTGAGAAAGCATCCGACTTCAAGAATCACAGCCTTCCACTTGCTAGAATTAAGAAAATAATGAAAGCTGATGAGGATGTTCGAATGATATCTGCCGAGGCTCCAGTTGTATTTGCAAAGGCATGTGAAATATTCATATTGGAGCTGACTTTGCGCTCATGGATTCACACAGAGGAGAACAAAAGGAGGACATTACAAAAGAATGATATCGCAGCTGCTATTTCAAGGACTGACGTCTTTGACTTTTTGGTTGATATTATCCCAAGAGATGAGTTGAAGGAAGAGGGGCTCGGAGTGACCAAGGCTACCCTCCCGGTGGTGGGTTCCCCAGCAGATGTTCCGTACTATTATGTTCCACCGCAGCATCCAGTGGGAGCTCCTGGGATGATCATGGGGAAGCCAGTTGATCAACCAGCACTGTATGCTGCTCAACAGCCTAGACCACCTGTGGCTTTCATGCCGTGGGCTCAGTCTCAACCTCAACAGCCGCAGCAACAGCAACAAGAAGCCCAACAGCAGCAGGCAGATACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005737 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016602 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFD_NFYB_HMF

Protein Analysis

269

Amino Acids

29.72

Weight (kDa)

5.33

Isoelectric Point (pI)

69.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Histone	PF00125	93 - 165	8.5e-18	Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF	PF00808	102 - 165	4.1e-16	Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008093)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56170 AT1G56170
fragaria_vesca	FvH4_3g14690 FvH4_3g14690 FvH4_3g14690
malus_domestica	MD05G1229600.v1.1 MD10G1208800.v1.1
prunus_persica	Prupe.4G132200_v2.0.a1
pyrus_communis	pycom05g21060 pycom10g17760
rosa_chinensis	RchiOBHm_Chr5g0024181
rosa_laevigata	RLG00000032777
rosa_multiflora	Rmu_co8304163.1_g000001 Rmu_sc0004033.1_g000008 Rmu_sc0039918.1_g000001
rosa_roxburghii	Rroxscaffold_1G00055060 Rroxscaffold_1G00055710
rosa_rugosa	Rorug05G0082300 Rorug05G0082400
rosa_samantha	Rh5AG172900 Rh5BG169500 Rh5CG185400 Rh5DG171900
rosa_wichuraiana	Rw5G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	510
AccB1I	GGYRCC	1 cut(s)	50
AciI	CCGC	3 cut(s)	226, 632, 761
AclWI	GGATC	2 cut(s)	12, 173
AcoI	YGGCCR	2 cut(s)	67, 138
AcyI	GRCGYC	1 cut(s)	507
AfaI	GTAC	1 cut(s)	617
AfiI	CCNNNNNNNGG	6 cut(s)	47, 157, 232, 589, 592, 593
AgsI	TTSAA	4 cut(s)	245, 295, 498, 550
AjnI	CCWGG	1 cut(s)	652
AluBI	AGCT	6 cut(s)	193, 241, 341, 418, 488, 650
AluI	AGCT	6 cut(s)	193, 241, 341, 418, 488, 650
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	1 cut(s)	750
AlwI	GGATC	2 cut(s)	12, 173
AoxI	GGCC	3 cut(s)	67, 138, 159
ApeKI	GCWGC	9 cut(s)	34, 37, 238, 485, 488, 634, 698, 763, 790
AspLEI	GCGC	1 cut(s)	429
AspS9I	GGNCC	2 cut(s)	160, 255
AsuC2I	CCSGG	1 cut(s)	587
AsuII	TTCGAA	1 cut(s)	355
AvaII	GGWCC	1 cut(s)	255
BalI	TGGCCA	1 cut(s)	69
BanI	GGYRCC	1 cut(s)	50
BanII	GRGCYC	3 cut(s)	564, 652, 742
Bbv12I	GWGCWC	1 cut(s)	652
BbvI	GCAGC	9 cut(s)	46, 49, 250, 475, 497, 646, 685, 775, 802
BccI	CCATC	2 cut(s)	41, 160
BceAI	ACGGC	3 cut(s)	38, 125, 718
BciT130I	CCWGG	1 cut(s)	654
BclI	TGATCA	2 cut(s)	660, 679
BcnI	CCSGG	1 cut(s)	587
BcoDI	GTCTC	1 cut(s)	750
BfaI	CTAG	2 cut(s)	318, 711
BfmI	CTRYAG	1 cut(s)	23
Bme1390I	CCNGG	2 cut(s)	587, 654
Bme18I	GGWCC	1 cut(s)	255
BmgT120I	GGNCC	2 cut(s)	160, 255
BmiI	GGNNCC	4 cut(s)	52, 162, 375, 598
BmrFI	CCNGG	2 cut(s)	587, 654
BmsI	GCATC	3 cut(s)	86, 293, 646
BoxI	GACNNNNGTC	1 cut(s)	506
BpmI	CTGGAG	1 cut(s)	360
Bpu14I	TTCGAA	1 cut(s)	355
BpuMI	CCSGG	1 cut(s)	587
BsaHI	GRCGYC	1 cut(s)	507
BsaJI	CCNNGG	4 cut(s)	369, 573, 653, 734
Bsc4I	CCNNNNNNNGG	6 cut(s)	47, 157, 232, 589, 592, 593
Bse1I	ACTGG	3 cut(s)	377, 641, 674
BseBI	CCWGG	1 cut(s)	654
BseDI	CCNNGG	4 cut(s)	369, 573, 653, 734
BseGI	GGATG	5 cut(s)	52, 284, 355, 637, 663
BseLI	CCNNNNNNNGG	6 cut(s)	47, 157, 232, 589, 592, 593
BseMII	CTCAG	2 cut(s)	24, 755
BseNI	ACTGG	3 cut(s)	377, 641, 674
BseRI	GAGGAG	1 cut(s)	461
BseXI	GCAGC	9 cut(s)	46, 49, 250, 475, 497, 646, 685, 775, 802
BseYI	CCCAGC	1 cut(s)	601
BsgI	GTGCAG	1 cut(s)	257
BshFI	GGCC	3 cut(s)	69, 140, 161
BshNI	GGYRCC	1 cut(s)	50
BsiHKAI	GWGCWC	1 cut(s)	652
BsiSI	CCGG	1 cut(s)	587
BslI	CCNNNNNNNGG	6 cut(s)	47, 157, 232, 589, 592, 593
BsmAI	GTCTC	1 cut(s)	750
BsnI	GGCC	3 cut(s)	69, 140, 161
Bsp119I	TTCGAA	1 cut(s)	355
Bsp1286I	GDGCHC	3 cut(s)	564, 652, 742
Bsp143I	GATC	4 cut(s)	4, 178, 660, 679
BspACI	CCGC	3 cut(s)	226, 632, 761
BspANI	GGCC	3 cut(s)	69, 140, 161
BspCNI	CTCAG	2 cut(s)	23, 754
BspLI	GGNNCC	4 cut(s)	52, 162, 375, 598
BspPI	GGATC	2 cut(s)	12, 173
BspT104I	TTCGAA	1 cut(s)	355
BspT107I	GGYRCC	1 cut(s)	50
BsrI	ACTGG	3 cut(s)	377, 641, 674
BssECI	CCNNGG	4 cut(s)	369, 573, 653, 734
BssMI	GATC	4 cut(s)	4, 178, 660, 679
BssNI	GRCGYC	1 cut(s)	507
BssT1I	CCWWGG	1 cut(s)	573
Bst2UI	CCWGG	1 cut(s)	654
Bst4CI	ACNGT	1 cut(s)	693
Bst6I	CTCTTC	1 cut(s)	549
BstACI	GRCGYC	1 cut(s)	507
BstAPI	GCANNNNNTGC	2 cut(s)	83, 695
BstBI	TTCGAA	1 cut(s)	355
BstC8I	GCNNGC	3 cut(s)	159, 195, 795
BstDEI	CTNAG	2 cut(s)	10, 741
BstDSI	CCRYGG	1 cut(s)	734
BstF5I	GGATG	5 cut(s)	52, 284, 355, 637, 663
BstHHI	GCGC	1 cut(s)	429
BstKTI	GATC	4 cut(s)	7, 181, 663, 682
BstMAI	GTCTC	1 cut(s)	750
BstMBI	GATC	4 cut(s)	4, 178, 660, 679
BstMWI	GCNNNNNNNGC	5 cut(s)	34, 83, 424, 695, 769
BstNI	CCWGG	1 cut(s)	654
BstNSI	RCATGY	1 cut(s)	399
BstPAI	GACNNNNGTC	1 cut(s)	506
BstSCI	CCNGG	2 cut(s)	585, 652
BstSFI	CTRYAG	1 cut(s)	23
BstV1I	GCAGC	9 cut(s)	46, 49, 250, 475, 497, 646, 685, 775, 802
BsuRI	GGCC	3 cut(s)	69, 140, 161
BtgI	CCRYGG	1 cut(s)	734
BtgZI	GCGATG	1 cut(s)	138
BtsCI	GGATG	5 cut(s)	52, 284, 355, 637, 663
BtsI	GCAGTG	1 cut(s)	141
BtsIMutI	CAGTG	3 cut(s)	141, 648, 689
Cac8I	GCNNGC	3 cut(s)	159, 195, 795
CfoI	GCGC	1 cut(s)	429
Cfr13I	GGNCC	2 cut(s)	160, 255
Csp6I	GTAC	1 cut(s)	616
CviAII	CATG	4 cut(s)	396, 432, 664, 730
CviQI	GTAC	1 cut(s)	616
DdeI	CTNAG	2 cut(s)	10, 741
DpnI	GATC	4 cut(s)	6, 180, 662, 681
DpnII	GATC	4 cut(s)	4, 178, 660, 679
EaeI	YGGCCR	2 cut(s)	67, 138
Eam1104I	CTCTTC	1 cut(s)	549
EarI	CTCTTC	1 cut(s)	549
Ecl136II	GAGCTC	1 cut(s)	650
Eco130I	CCWWGG	1 cut(s)	573
Eco24I	GRGCYC	3 cut(s)	564, 652, 742
Eco32I	GATATC	2 cut(s)	363, 481
Eco47I	GGWCC	1 cut(s)	255
Eco53kI	GAGCTC	1 cut(s)	650
EcoICRI	GAGCTC	1 cut(s)	650
EcoO109I	RGGNCCY	1 cut(s)	160
EcoRII	CCWGG	1 cut(s)	652
EcoRV	GATATC	2 cut(s)	363, 481
EcoT14I	CCWWGG	1 cut(s)	573
EcoT38I	GRGCYC	3 cut(s)	564, 652, 742
ErhI	CCWWGG	1 cut(s)	573
FaeI	CATG	4 cut(s)	399, 435, 667, 733
FatI	CATG	4 cut(s)	395, 431, 663, 729
FauNDI	CATATG	1 cut(s)	82
FbaI	TGATCA	2 cut(s)	660, 679
FokI	GGATG	5 cut(s)	59, 271, 362, 624, 670
FriOI	GRGCYC	3 cut(s)	564, 652, 742
FspBI	CTAG	2 cut(s)	318, 711
GlaI	GCGC	1 cut(s)	428
GsaI	CCCAGC	1 cut(s)	605
GsuI	CTGGAG	1 cut(s)	360
HaeIII	GGCC	3 cut(s)	69, 140, 161
HapII	CCGG	1 cut(s)	587
HhaI	GCGC	1 cut(s)	429
Hin1I	GRCGYC	1 cut(s)	507
Hin1II	CATG	4 cut(s)	399, 435, 667, 733
Hin6I	GCGC	1 cut(s)	427
HinP1I	GCGC	1 cut(s)	427
HinfI	GANTC	2 cut(s)	298, 436
HpaII	CCGG	1 cut(s)	587
Hpy188I	TCNGA	4 cut(s)	13, 259, 289, 566
Hpy188III	TCNNGA	3 cut(s)	123, 245, 295
HpyAV	CCTTC	3 cut(s)	99, 317, 544
HpyCH4III	ACNGT	1 cut(s)	693
HpyCH4IV	ACGT	2 cut(s)	273, 507
HpyCH4V	TGCA	4 cut(s)	134, 238, 268, 389
HpyF10VI	GCNNNNNNNGC	5 cut(s)	34, 83, 424, 695, 769
HpyF3I	CTNAG	2 cut(s)	10, 741
HpySE526I	ACGT	2 cut(s)	273, 507
Hsp92I	GRCGYC	1 cut(s)	507
Hsp92II	CATG	4 cut(s)	399, 435, 667, 733
HspAI	GCGC	1 cut(s)	427
Ksp22I	TGATCA	2 cut(s)	660, 679
Kzo9I	GATC	4 cut(s)	4, 178, 660, 679
LmnI	GCTCC	6 cut(s)	91, 112, 379, 415, 647, 655
Lsp1109I	GCAGC	9 cut(s)	46, 49, 250, 475, 497, 646, 685, 775, 802
LweI	GCATC	3 cut(s)	86, 293, 646
MaeI	CTAG	2 cut(s)	318, 711
MaeII	ACGT	2 cut(s)	273, 507
MaeIII	GTNAC	3 cut(s)	142, 183, 568
MalI	GATC	4 cut(s)	6, 180, 662, 681
MboI	GATC	4 cut(s)	4, 178, 660, 679
MboII	GAAGA	1 cut(s)	566
MhlI	GDGCHC	3 cut(s)	564, 652, 742
MlsI	TGGCCA	1 cut(s)	69
MluCI	AATT	1 cut(s)	321
MluNI	TGGCCA	1 cut(s)	69
MmeI	TCCRAC	2 cut(s)	282, 312
MnlI	CCTC	7 cut(s)	340, 364, 439, 453, 550, 593, 762
Mox20I	TGGCCA	1 cut(s)	69
MscI	TGGCCA	1 cut(s)	69
MseI	TTAA	2 cut(s)	324, 805
Msp20I	TGGCCA	1 cut(s)	69
MspA1I	CMGCKG	1 cut(s)	488
MspI	CCGG	1 cut(s)	587
MspR9I	CCNGG	2 cut(s)	587, 654
MvaI	CCWGG	1 cut(s)	654
MwoI	GCNNNNNNNGC	5 cut(s)	34, 83, 424, 695, 769
NciI	CCSGG	1 cut(s)	587
NdeI	CATATG	1 cut(s)	82
NdeII	GATC	4 cut(s)	4, 178, 660, 679
NlaIII	CATG	4 cut(s)	399, 435, 667, 733
NlaIV	GGNNCC	4 cut(s)	52, 162, 375, 598
NmeAIII	GCCGAG	1 cut(s)	394
NmuCI	GTSAC	3 cut(s)	142, 183, 568
NspI	RCATGY	1 cut(s)	399
NspV	TTCGAA	1 cut(s)	355
PfeI	GAWTC	2 cut(s)	298, 436
PshAI	GACNNNNGTC	1 cut(s)	506
Psp124BI	GAGCTC	1 cut(s)	652
Psp6I	CCWGG	1 cut(s)	652
PspFI	CCCAGC	1 cut(s)	601
PspGI	CCWGG	1 cut(s)	652
PspN4I	GGNNCC	4 cut(s)	52, 162, 375, 598
PspPI	GGNCC	2 cut(s)	160, 255
PsrI	GAACNNNNNNTAC	1 cut(s)	38
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	1 cut(s)	617
RsaNI	GTAC	1 cut(s)	616
SacI	GAGCTC	1 cut(s)	652
SaqAI	TTAA	2 cut(s)	324, 805
Sau3AI	GATC	4 cut(s)	4, 178, 660, 679
Sau96I	GGNCC	2 cut(s)	160, 255
ScrFI	CCNGG	2 cut(s)	587, 654
SduI	GDGCHC	3 cut(s)	564, 652, 742
SfaNI	GCATC	3 cut(s)	86, 293, 646
SfcI	CTRYAG	1 cut(s)	23
SfuI	TTCGAA	1 cut(s)	355
SinI	GGWCC	1 cut(s)	255
Sse9I	AATT	1 cut(s)	321
SsiI	CCGC	3 cut(s)	226, 632, 761
SspI	AATATT	1 cut(s)	405
SspMI	CTAG	2 cut(s)	318, 711
SstI	GAGCTC	1 cut(s)	652
StyD4I	CCNGG	2 cut(s)	585, 652
StyI	CCWWGG	1 cut(s)	573
TaaI	ACNGT	1 cut(s)	693
TaiI	ACGT	2 cut(s)	276, 510
TaqI	TCGA	1 cut(s)	355
TasI	AATT	1 cut(s)	321
TauI	GCSGC	1 cut(s)	763
TfiI	GAWTC	2 cut(s)	298, 436
Tru1I	TTAA	2 cut(s)	324, 805
Tru9I	TTAA	2 cut(s)	324, 805
TscAI	CASTG	3 cut(s)	141, 648, 696
TseFI	GTSAC	3 cut(s)	142, 183, 568
TseI	GCWGC	9 cut(s)	34, 37, 238, 485, 488, 634, 698, 763, 790
Tsp45I	GTSAC	3 cut(s)	142, 183, 568
TspDTI	ATGAA	3 cut(s)	350, 397, 718
TspGWI	ACGGA	2 cut(s)	171, 603
TspRI	CASTG	3 cut(s)	141, 648, 696
VpaK11BI	GGWCC	1 cut(s)	255
XceI	RCATGY	1 cut(s)	399
XspI	CTAG	2 cut(s)	318, 711
ZraI	GACGTC	1 cut(s)	508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.