MD05G1232600.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018904
Physical Location & Seq
Forward (+)
36552297 .. 36552851
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232600.v1.1.491

Sequence Viewer

Length: 555 bp
ATGAATTTAATGAAAAATCCATTGAAAGCTTCAACTAAGATGTGCATGCTCATCTTCTACTCTTCTTTGCTGCTCATCTTCGCAGCAGTATTCTCCATGGCTGATGATGCCACGAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCAACTGGCGGTACGTTTGAGCTAGGATTTTGCAGTCCTGATGCTTCTAATAGACGGTACGTGGGGATATGGTACAAGCACATATCTGTTACAACCATTGTATGGGTTGCCAACAGAGACAAGCCCCTCGCCGATTTGTCCGGCATTCTGAAGGTCACCAACCCCGGAATTCTTGTCCTAAACCACAACATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACTCTGAGAACTTTCTGTGGCAAAGTTTTGATACCCTGGCGATAATTTCTTACCAGGTAATGAAGATTGGTTTTAGGAACACAGTTACAGGCTTCCAATTGGCATCTTAG

Protein Analysis

185

Amino Acids

20.35

Weight (kDa)

6.71

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 83 - 175 4.4e-27 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018904)

Species Orthologous Gene IDs
malus_domestica MD05G1220400.v1.1 MD05G1231900.v1.1 MD05G1232600.v1.1 MD12G1047200.v1.1
pyrus_communis pycom05g19770 pycom05g20310 pycom05g20350
rosa_chinensis RchiOBHm_Chr3g0484821
rosa_samantha Rh3DG293100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 255
AciI CCGC 1 cut(s) 162
AcsI RAATTY 2 cut(s) 4, 321
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 4 cut(s) 118, 166, 212, 227
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 3 cut(s) 25, 33, 156
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 2 cut(s) 480, 498
AluBI AGCT 2 cut(s) 29, 175
AluI AGCT 2 cut(s) 29, 175
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 3 cut(s) 129, 137, 264
ApeKI GCWGC 2 cut(s) 70, 83
ApoI RAATTY 2 cut(s) 4, 321
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 152, 301
AvaII GGWCC 1 cut(s) 357
BauI CACGAG 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 2 cut(s) 57, 95
BciT130I CCWGG 2 cut(s) 482, 500
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 3 cut(s) 129, 137, 264
BfaI CTAG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 147
BisI GCNGC 2 cut(s) 71, 84
BlsI GCNGC 2 cut(s) 72, 85
Bme1390I CCNGG 3 cut(s) 318, 482, 500
Bme18I GGWCC 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 357
BmrFI CCNGG 3 cut(s) 318, 482, 500
BmsI GCATC 2 cut(s) 97, 184
BpuMI CCSGG 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 96, 316, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse1I ACTGG 2 cut(s) 163, 389
BseBI CCWGG 2 cut(s) 482, 500
BseDI CCNNGG 3 cut(s) 96, 316, 480
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 2 cut(s) 163, 389
BseXI GCAGC 2 cut(s) 57, 95
BsgI GTGCAG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 2 cut(s) 294, 318
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 3 cut(s) 129, 137, 264
BsmBI CGTCTC 1 cut(s) 129
BsmI GAATGC 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 350
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 442
BsrI ACTGG 2 cut(s) 163, 389
BssECI CCNNGG 3 cut(s) 96, 316, 480
BssSI CACGAG 1 cut(s) 112
BssT1I CCWWGG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 112
Bst2UI CCWGG 2 cut(s) 482, 500
Bst4CI ACNGT 5 cut(s) 140, 148, 210, 352, 529
Bst6I CTCTTC 1 cut(s) 67
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 47
BstDEI CTNAG 3 cut(s) 36, 450, 552
BstDSI CCRYGG 1 cut(s) 96
BstEII GGTNACC 1 cut(s) 307
BstMAI GTCTC 3 cut(s) 129, 137, 264
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 2 cut(s) 482, 500
BstNSI RCATGY 1 cut(s) 49
BstPI GGTNACC 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 316, 480, 498
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 57, 95
BtgI CCRYGG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 357
CsiI ACCWGGT 1 cut(s) 498
Csp6I GTAC 4 cut(s) 117, 165, 211, 226
CviAII CATG 3 cut(s) 46, 97, 343
CviJI RGCY 5 cut(s) 29, 101, 175, 277, 537
CviKI_1 RGCY 5 cut(s) 29, 101, 175, 277, 537
CviQI GTAC 4 cut(s) 117, 165, 211, 226
DdeI CTNAG 3 cut(s) 36, 450, 552
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 323
Eco91I GGTNACC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 480, 498
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 3 cut(s) 49, 100, 346
FaiI YATR 6 cut(s) 47, 98, 223, 236, 256, 344
FatI CATG 3 cut(s) 45, 96, 342
Fnu4HI GCNGC 2 cut(s) 71, 84
Fsp4HI GCNGC 2 cut(s) 71, 84
FspBI CTAG 1 cut(s) 176
GluI GCNGC 2 cut(s) 71, 84
HapII CCGG 2 cut(s) 294, 318
Hin1II CATG 3 cut(s) 49, 100, 346
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 3 cut(s) 385, 407, 446
HpaII CCGG 2 cut(s) 294, 318
HphI GGTGA 2 cut(s) 152, 301
Hpy188I TCNGA 2 cut(s) 303, 451
Hpy188III TCNNGA 3 cut(s) 191, 372, 424
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 5 cut(s) 140, 148, 210, 352, 529
HpyCH4IV ACGT 3 cut(s) 167, 213, 397
HpyCH4V TGCA 3 cut(s) 45, 186, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 3 cut(s) 36, 450, 552
HpySE526I ACGT 3 cut(s) 167, 213, 397
Hsp92II CATG 3 cut(s) 49, 100, 346
Lsp1109I GCAGC 2 cut(s) 57, 95
LweI GCATC 2 cut(s) 97, 184
MabI ACCWGGT 1 cut(s) 498
MaeI CTAG 1 cut(s) 176
MaeII ACGT 3 cut(s) 167, 213, 397
MaeIII GTNAC 3 cut(s) 241, 307, 529
MboII GAAGA 4 cut(s) 46, 54, 70, 520
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 4 cut(s) 4, 321, 489, 542
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 2 cut(s) 290, 370
MseI TTAA 1 cut(s) 8
MspI CCGG 2 cut(s) 294, 318
MspR9I CCNGG 3 cut(s) 318, 482, 500
MunI CAATTG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 297
MvaI CCWGG 2 cut(s) 482, 500
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 96
NlaIII CATG 3 cut(s) 49, 100, 346
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PctI GAATGC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 385, 407
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 2 cut(s) 72, 85
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Ppu21I YACGTR 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 480, 498
PspEI GGTNACC 1 cut(s) 307
PspGI CCWGG 2 cut(s) 480, 498
PspPI GGNCC 1 cut(s) 357
RsaI GTAC 4 cut(s) 118, 166, 212, 227
RsaNI GTAC 4 cut(s) 117, 165, 211, 226
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 2 cut(s) 71, 84
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 318, 482, 500
SduI GDGCHC 1 cut(s) 350
SetI ASST 7 cut(s) 31, 170, 177, 216, 309, 400, 504
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 2 cut(s) 97, 184
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 1 cut(s) 357
SphI GCATGC 1 cut(s) 49
Sse9I AATT 4 cut(s) 4, 321, 489, 542
SsiI CCGC 1 cut(s) 162
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 316, 480, 498
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 5 cut(s) 140, 148, 210, 352, 529
TaiI ACGT 3 cut(s) 170, 216, 400
TaqI TCGA 1 cut(s) 371
TasI AATT 4 cut(s) 4, 321, 489, 542
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 2 cut(s) 385, 407
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 70, 83
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 17, 26, 521
TspRI CASTG 1 cut(s) 396
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 4, 321
XceI RCATGY 1 cut(s) 49
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.