MD12G1047200.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018904
Physical Location & Seq
Forward (+)
5434648 .. 5434980
333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1047200.v1.1.491

Sequence Viewer

Length: 333 bp
ATGCAATCCTTAACAAGGTTTTTCATGTCTTTGTTTTTATTCTTCTTCCTAAGAATCGCATCTGCTGCAACGTCGGGAACAATCACTCCTAGTCGATCTATTGGAGATGGCGAAACTCTGGTTTCGACGAAAGGAGGCTTTGAACTGGGATTCTCCAGCCTTGGTAACTCAAAAGGCCGGTACTTGGGGATATGGTACACAGTTTCTACTGATACAATTGTTTGGGTAGCCAACAGAGAAACACCACTTGGTGATTCTTCAGGACTTTTGAATCTCACTCAGCAAGGAGTTTTAGTCTTCCTTAATAGCTCAAACAGCATTGTATGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

111

Amino Acids

11.93

Weight (kDa)

8.19

Isoelectric Point (pI)

25.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 110 1.3e-10 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018904)

Species Orthologous Gene IDs
malus_domestica MD05G1220400.v1.1 MD05G1231900.v1.1 MD05G1232600.v1.1 MD12G1047200.v1.1
pyrus_communis pycom05g19770 pycom05g20310 pycom05g20350
rosa_chinensis RchiOBHm_Chr3g0484821
rosa_samantha Rh3DG293100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 243
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 2 cut(s) 182, 197
AfiI CCNNNNNNNGG 2 cut(s) 15, 184
AgsI TTSAA 2 cut(s) 143, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AloI GAACNNNNNNTCC 2 cut(s) 70, 102
AluBI AGCT 1 cut(s) 309
AluI AGCT 1 cut(s) 309
AoxI GGCC 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 52
BccI CCATC 1 cut(s) 101
BfaI CTAG 1 cut(s) 90
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 289
BpmI CTGGAG 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 184
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 160
BseLI CCNNNNNNNGG 2 cut(s) 15, 184
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 52
BshFI GGCC 1 cut(s) 177
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 15, 184
BsnI GGCC 1 cut(s) 177
Bsp143I GATC 1 cut(s) 95
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 50, 279
BstENI CCTNNNNNAGG 1 cut(s) 13
BstKTI GATC 1 cut(s) 98
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 65, 315
BstV1I GCAGC 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 289
BsuRI GGCC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 177
Csp6I GTAC 2 cut(s) 181, 196
CviAII CATG 1 cut(s) 25
CviJI RGCY 5 cut(s) 138, 159, 177, 230, 309
CviKI_1 RGCY 5 cut(s) 138, 159, 177, 230, 309
CviQI GTAC 2 cut(s) 181, 196
DdeI CTNAG 2 cut(s) 50, 279
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
DraIII CACNNNGTG 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 1 cut(s) 28
FaiI YATR 4 cut(s) 26, 193, 325, 331
FatI CATG 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 90
GluI GCNGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 1 cut(s) 177
HapII CCGG 1 cut(s) 178
Hin1II CATG 1 cut(s) 28
HinfI GANTC 4 cut(s) 54, 150, 254, 271
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 75, 261
Hpy8I GTNNAC 1 cut(s) 198
Hpy99I CGWCG 2 cut(s) 76, 130
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 2 cut(s) 4, 68
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 315
HpyF3I CTNAG 2 cut(s) 50, 279
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 1 cut(s) 28
Kzo9I GATC 1 cut(s) 95
LpnPI CCDG 5 cut(s) 104, 131, 169, 191, 246
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 1 cut(s) 68
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 4 cut(s) 34, 37, 249, 289
MfeI CAATTG 1 cut(s) 216
MluCI AATT 1 cut(s) 216
MnlI CCTC 1 cut(s) 128
MseI TTAA 2 cut(s) 11, 303
MspI CCGG 1 cut(s) 178
MunI CAATTG 1 cut(s) 216
MwoI GCNNNNNNNGC 2 cut(s) 65, 315
NdeII GATC 1 cut(s) 95
NlaIII CATG 1 cut(s) 28
PfeI GAWTC 4 cut(s) 54, 150, 254, 271
PkrI GCNGC 1 cut(s) 67
RsaI GTAC 2 cut(s) 182, 197
RsaNI GTAC 2 cut(s) 181, 196
SaqAI TTAA 2 cut(s) 11, 303
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 95
SetI ASST 3 cut(s) 20, 74, 311
SfaNI GCATC 1 cut(s) 68
Sse9I AATT 1 cut(s) 216
SspMI CTAG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 94, 125
TasI AATT 1 cut(s) 216
TfiI GAWTC 4 cut(s) 54, 150, 254, 271
Tru1I TTAA 2 cut(s) 11, 303
Tru9I TTAA 2 cut(s) 11, 303
TseI GCWGC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 13
XagI CCTNNNNNAGG 1 cut(s) 13
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.