pycom05g20310

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018904
Physical Location & Seq
Reverse (-)
23110783 .. 23111421
639 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20310.1

Sequence Viewer

Length: 639 bp
ATGACCCAATTATTGAATGAAAGAAGCAGTTATGCAGCTTCTTCTTTTCTCTTCGATTCCTCTGGTTTCTTTGACTTCTCTCTCGTTTCAGTAGATTACTTCTCTGTTGACATATCATACGTTTATACCACATTTCAGATCCCACCAAGGCAAAGAAAAATCCCAATTAAGATGTGCTTGCATCTTTTGCTGCTCTTCCTCCTCGTCACTACTGCTGCAACATTTTCCGCAGCACAAGTCAACGATGCAACCACATTTCAGTCAATTGCAGATGGTGAGACCATAATCTCAGATGGTGGCACCTTTGAGCTTGGTTTTTTCAGTCCCGGTGCTTCAAATAAACGCTACGTGGGGATATGGTACAAGAAGATACCGGATACACTGATGGATGGACAAGAAATAGCTGTGAAAAAGCTATCGAAACATTCGAGGCAAGGACTCAACGAGTTAAAGAATGAGGTTACACATATTGCCGAACTTCAGCACTGGAATCTAGTGAGGCTTCTAGGATGCTGCATTCAAGAAGACGAGATGATGCTGATCGACGAGTTCATGCCTAACAAAAGCTTAGACTTCTTTATTTTCGGTTTGTTTCCCATTTGTAAACTTCCTATGATCCTTTGTCGATTGGCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

213

Amino Acids

24.01

Weight (kDa)

5.55

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 120 - 193 2e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 130 - 193 1.6e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018904)

Species Orthologous Gene IDs
malus_domestica MD05G1220400.v1.1 MD05G1231900.v1.1 MD05G1232600.v1.1 MD12G1047200.v1.1
pyrus_communis pycom05g19770 pycom05g20310 pycom05g20350
rosa_chinensis RchiOBHm_Chr3g0484821
rosa_samantha Rh3DG293100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AciI CCGC 1 cut(s) 228
AclWI GGATC 2 cut(s) 133, 610
AcuI CTGAAG 1 cut(s) 464
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 3 cut(s) 16, 336, 521
AluBI AGCT 5 cut(s) 38, 310, 404, 415, 567
AluI AGCT 5 cut(s) 38, 310, 404, 415, 567
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 2 cut(s) 133, 610
ApeKI GCWGC 5 cut(s) 35, 190, 215, 230, 513
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 287
BanI GGYRCC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 5 cut(s) 47, 177, 202, 242, 500
BccI CCATC 4 cut(s) 266, 287, 379, 383
BciVI GTATCC 1 cut(s) 370
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 2 cut(s) 494, 506
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 5 cut(s) 36, 191, 216, 231, 514
BlsI GCNGC 5 cut(s) 37, 192, 217, 232, 515
Bme1390I CCNGG 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 4 cut(s) 190, 235, 500, 525
BpiI GAAGAC 1 cut(s) 531
BpuMI CCSGG 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 539
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 394, 515
BseJI GATNNNNATC 1 cut(s) 539
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 5 cut(s) 47, 177, 202, 242, 500
BshNI GGYRCC 1 cut(s) 299
BsiSI CCGG 2 cut(s) 327, 374
BslFI GGGAC 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 516
Bso31I GGTCTC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 138, 540, 615
BspACI CCGC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 302
BspLI GGNNCC 1 cut(s) 301
BspPI GGATC 2 cut(s) 133, 610
BspQI GCTCTTC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 138, 540, 615
BssT1I CCWWGG 1 cut(s) 146
Bst6I CTCTTC 2 cut(s) 56, 200
BstAPI GCANNNNNTGC 1 cut(s) 187
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 179
BstDEI CTNAG 2 cut(s) 289, 568
BstF5I GGATG 2 cut(s) 394, 515
BstKTI GATC 3 cut(s) 141, 543, 618
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 3 cut(s) 138, 540, 615
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 325
BstV1I GCAGC 5 cut(s) 47, 177, 202, 242, 500
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BsuI GTATCC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 394, 515
BtsIMutI CAGTG 2 cut(s) 380, 484
Cac8I GCNNGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 553
CviJI RGCY 7 cut(s) 38, 310, 404, 415, 502, 567, 632
CviKI_1 RGCY 7 cut(s) 38, 310, 404, 415, 502, 567, 632
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 2 cut(s) 289, 568
DpnI GATC 3 cut(s) 140, 542, 617
DpnII GATC 3 cut(s) 138, 540, 615
Eam1104I CTCTTC 2 cut(s) 56, 200
EarI CTCTTC 2 cut(s) 56, 200
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 1 cut(s) 556
FaiI YATR 9 cut(s) 33, 113, 118, 126, 284, 358, 468, 554, 614
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 309
FatI CATG 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 36, 191, 216, 231, 514
FokI GGATG 2 cut(s) 401, 522
Fsp4HI GCNGC 5 cut(s) 36, 191, 216, 231, 514
FspBI CTAG 2 cut(s) 494, 506
GluI GCNGC 5 cut(s) 36, 191, 216, 231, 514
HapII CCGG 2 cut(s) 327, 374
Hin1II CATG 1 cut(s) 556
HincII GTYRAC 2 cut(s) 109, 241
HindII GTYRAC 2 cut(s) 109, 241
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 3 cut(s) 56, 438, 490
HpaII CCGG 2 cut(s) 327, 374
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 3 cut(s) 109, 241, 605
Hpy188I TCNGA 2 cut(s) 138, 292
Hpy188III TCNNGA 1 cut(s) 521
Hpy8I GTNNAC 3 cut(s) 109, 241, 605
Hpy99I CGWCG 1 cut(s) 548
HpyCH4IV ACGT 2 cut(s) 120, 348
HpyCH4V TGCA 6 cut(s) 35, 181, 218, 248, 269, 516
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 2 cut(s) 289, 568
HpySE526I ACGT 2 cut(s) 120, 348
Hsp92II CATG 1 cut(s) 556
Kzo9I GATC 3 cut(s) 138, 540, 615
LguI GCTCTTC 1 cut(s) 200
LpnPI CCDG 4 cut(s) 48, 340, 387, 472
Lsp1109I GCAGC 5 cut(s) 47, 177, 202, 242, 500
LweI GCATC 4 cut(s) 190, 235, 500, 525
MaeI CTAG 2 cut(s) 494, 506
MaeII ACGT 2 cut(s) 120, 348
MaeIII GTNAC 2 cut(s) 205, 460
MalI GATC 3 cut(s) 140, 542, 617
MboI GATC 3 cut(s) 138, 540, 615
MboII GAAGA 5 cut(s) 33, 43, 187, 379, 536
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 138
MluCI AATT 3 cut(s) 8, 165, 264
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 6 cut(s) 70, 209, 212, 423, 451, 492
MseI TTAA 2 cut(s) 168, 449
MspI CCGG 2 cut(s) 327, 374
MspR9I CCNGG 1 cut(s) 327
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 516
MwoI GCNNNNNNNGC 1 cut(s) 187
NciI CCSGG 1 cut(s) 327
NdeII GATC 3 cut(s) 138, 540, 615
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 56, 490
PkrI GCNGC 5 cut(s) 37, 192, 217, 232, 515
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Ppu21I YACGTR 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 301
PsuI RGATCY 1 cut(s) 138
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 168, 449
SatI GCNGC 5 cut(s) 36, 191, 216, 231, 514
Sau3AI GATC 3 cut(s) 138, 540, 615
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 1 cut(s) 327
SetI ASST 9 cut(s) 40, 123, 305, 312, 351, 406, 417, 462, 569
SfaNI GCATC 4 cut(s) 190, 235, 500, 525
Sse9I AATT 3 cut(s) 8, 165, 264
SsiI CCGC 1 cut(s) 228
SspMI CTAG 2 cut(s) 494, 506
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 2 cut(s) 123, 351
TaqI TCGA 5 cut(s) 54, 419, 428, 543, 625
TasI AATT 3 cut(s) 8, 165, 264
TfiI GAWTC 2 cut(s) 56, 490
Tru1I TTAA 2 cut(s) 168, 449
Tru9I TTAA 2 cut(s) 168, 449
TscAI CASTG 2 cut(s) 387, 491
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 5 cut(s) 35, 190, 215, 230, 513
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 33, 541
TspRI CASTG 2 cut(s) 387, 491
XspI CTAG 2 cut(s) 494, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.