Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010156

Physical Location & Seq

Genomic Coordinates Forward (+)

38246043 .. 38249704

Gene Structure 3662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1250100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGGAAGGGGCAAGATAACTATTTCGAGGATAGAAAACCGAACCACAAGGCAAGTGACTTTTGCGAAACGGCGGGCAGGGCTGATCAAGAAGACTCATGAGCTTTCTGTGCTATGTGATGCCCAGATCGGCCTTGTCATTTTCTCAAGCACTGGCAAGATGTTTCAGTACTGCAGTGAGTCATCAAGCATGGAGGAGCTTATTCAAAGATACCAGGTTACAAAAGGGATTCGAGTACCGCAACACAACGACACGGAATATATGAATGCTGAGTTGAGAAAAATGAGGAGAGAAACTCAGCATCTGGAATTGAGTTTGCAACGCTACACGGGCGAGGATTTACGCTGCGTTCGATTTGATGATCTGGTGGAGCTTGAACACCAGCTGGAGGAGTCTGTTAACAGGGTTCGAGCTAGGAAGTTTGAGGTCTTGCAACAGCAGACAGACAATCTTCTTGCAACGACCAAAAGACTGGAGGAAGAAAATGAGCAACTATTCCATCTGATTAAGGCGCATCAGGCTGCAGCATTTGGTCATGATCAGGCGCATCTTGAGGCTCATGAGCAGGTGGAGAATCTGGAGATGCTGCCGAAATCTGAAGAGCATCGACATGTGCTCGATCAATTCCCATTTGGAGGTGAAGAACAACCAAGTAGTGTGCTTCAGCTTGCTACTTTGCAGCCTCAGCCGCAGTTCAACCCTTACCCTCACCAGCCTGATCATCATCATCCTGGCCTTCTCGATTTCAATCTGAGCGGCCCTTCTATGTACGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009791 GO:0009987 GO:0010154 GO:0010941 GO:0010942 GO:0016051 GO:0019252 GO:0022414 GO:0032501 GO:0032502 GO:0033692 GO:0034637 GO:0034645 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044464 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 ko04013 ko04022 ko04371 ko05418 map00260 map00630 map01100 map01110 map01130 map01200 map04013 map04022 map04371 map05418

Pfam Domains

Protein Families

K-box SRF-TF

Protein Analysis

259

Amino Acids

30.01

Weight (kDa)

6.02

Isoelectric Point (pI)

53.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SRF-TF	PF00319	10 - 57	2.9e-23	SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box	PF01486	81 - 167	1.8e-18	K-box region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23260 AT5G23260 AT5G23260 AT5G23260
fragaria_vesca	FvH4_3g12460 FvH4_3g12460 FvH4_3g12460
malus_domestica	MD05G1250100.v1.1 MD10G1231100.v1.1
prunus_persica	Prupe.4G113500_v2.0.a1
pyrus_communis	pycom10g19430
rosa_chinensis	RchiOBHm_Chr5g0020391
rosa_laevigata	RLG00000032519
rosa_multiflora	Rmu_sc0000256.1_g000010
rosa_roxburghii	Rroxscaffold_1G00057870
rosa_rugosa	Rorug05G0058900 Rorug05G0058900
rosa_samantha	Rh5AG149200 Rh5BG148900 Rh5DG147600
rosa_wichuraiana	Rw5G013230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	556
Acc36I	ACCTGC	1 cut(s)	556
AccBSI	CCGCTC	1 cut(s)	756
AciI	CCGC	4 cut(s)	73, 239, 689, 756
AcuI	CTGAAG	2 cut(s)	618, 647
AfaI	GTAC	3 cut(s)	170, 237, 770
AfiI	CCNNNNNNNGG	2 cut(s)	388, 635
AflIII	ACRYGT	1 cut(s)	610
AgsI	TTSAA	4 cut(s)	206, 377, 697, 748
AjnI	CCWGG	2 cut(s)	213, 730
AluBI	AGCT	6 cut(s)	103, 199, 373, 385, 413, 667
AluI	AGCT	6 cut(s)	103, 199, 373, 385, 413, 667
Alw21I	GWGCWC	1 cut(s)	618
AlwNI	CAGNNNCTG	1 cut(s)	304
AoxI	GGCC	3 cut(s)	130, 733, 757
ApeKI	GCWGC	5 cut(s)	345, 521, 524, 586, 679
AspLEI	GCGC	2 cut(s)	514, 547
AspS9I	GGNCC	1 cut(s)	758
AsuHPI	GGTGA	2 cut(s)	650, 701
BbsI	GAAGAC	1 cut(s)	98
Bbv12I	GWGCWC	1 cut(s)	618
BbvCI	CCTCAGC	1 cut(s)	684
BbvI	GCAGC	5 cut(s)	332, 508, 536, 573, 691
BccI	CCATC	1 cut(s)	507
BceAI	ACGGC	1 cut(s)	86
BciT130I	CCWGG	2 cut(s)	215, 732
BclI	TGATCA	3 cut(s)	84, 538, 718
BfaI	CTAG	1 cut(s)	414
BfmI	CTRYAG	2 cut(s)	172, 522
BfuAI	ACCTGC	1 cut(s)	556
BisI	GCNGC	7 cut(s)	346, 522, 525, 587, 680, 689, 757
BlsI	GCNGC	7 cut(s)	347, 523, 526, 588, 681, 690, 758
BmcAI	AGTACT	1 cut(s)	170
Bme1390I	CCNGG	2 cut(s)	215, 732
BmgT120I	GGNCC	1 cut(s)	758
BmrFI	CCNGG	2 cut(s)	215, 732
BmsI	GCATC	6 cut(s)	109, 310, 523, 556, 573, 613
BpiI	GAAGAC	1 cut(s)	98
BplI	GAGNNNNNCTC	2 cut(s)	280, 312
BpmI	CTGGAG	3 cut(s)	407, 494, 599
Bpu10I	CCTNAGC	1 cut(s)	684
BpuEI	CTTGAG	2 cut(s)	130, 572
BsaBI	GATNNNNATC	2 cut(s)	723, 747
Bsc4I	CCNNNNNNNGG	2 cut(s)	388, 635
Bse1I	ACTGG	2 cut(s)	157, 477
Bse8I	GATNNNNATC	2 cut(s)	723, 747
BseBI	CCWGG	2 cut(s)	215, 732
BseGI	GGATG	1 cut(s)	727
BseJI	GATNNNNATC	2 cut(s)	723, 747
BseLI	CCNNNNNNNGG	2 cut(s)	388, 635
BseMII	CTCAG	4 cut(s)	261, 311, 698, 743
BseNI	ACTGG	2 cut(s)	157, 477
BseRI	GAGGAG	3 cut(s)	209, 301, 404
BseXI	GCAGC	5 cut(s)	332, 508, 536, 573, 691
BshFI	GGCC	3 cut(s)	132, 735, 759
BsiHKAI	GWGCWC	1 cut(s)	618
BslI	CCNNNNNNNGG	2 cut(s)	388, 635
BsmI	GAATGC	1 cut(s)	271
BsnI	GGCC	3 cut(s)	132, 735, 759
Bsp1286I	GDGCHC	1 cut(s)	618
Bsp143I	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
BspACI	CCGC	4 cut(s)	73, 239, 689, 756
BspANI	GGCC	3 cut(s)	132, 735, 759
BspCNI	CTCAG	4 cut(s)	262, 310, 697, 744
BspHI	TCATGA	3 cut(s)	97, 535, 559
BspMAI	CTGCAG	2 cut(s)	176, 526
BspMI	ACCTGC	1 cut(s)	556
BspQI	GCTCTTC	1 cut(s)	594
BsrBI	CCGCTC	1 cut(s)	756
BsrI	ACTGG	2 cut(s)	157, 477
BssMI	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
Bst2UI	CCWGG	2 cut(s)	215, 732
Bst6I	CTCTTC	1 cut(s)	594
BstC8I	GCNNGC	2 cut(s)	75, 669
BstDEI	CTNAG	4 cut(s)	270, 297, 684, 752
BstF5I	GGATG	1 cut(s)	727
BstHHI	GCGC	2 cut(s)	514, 547
BstKTI	GATC	6 cut(s)	87, 129, 364, 541, 622, 721
BstMBI	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
BstMWI	GCNNNNNNNGC	6 cut(s)	79, 109, 330, 518, 685, 688
BstNI	CCWGG	2 cut(s)	215, 732
BstNSI	RCATGY	1 cut(s)	614
BstSCI	CCNGG	2 cut(s)	213, 730
BstSFI	CTRYAG	2 cut(s)	172, 522
BstV1I	GCAGC	5 cut(s)	332, 508, 536, 573, 691
BstV2I	GAAGAC	1 cut(s)	98
BstXI	CCANNNNNNTGG	1 cut(s)	472
BsuRI	GGCC	3 cut(s)	132, 735, 759
BtsCI	GGATG	1 cut(s)	727
BtsI	GCAGTG	1 cut(s)	181
BtsIMutI	CAGTG	2 cut(s)	150, 181
BveI	ACCTGC	1 cut(s)	556
Cac8I	GCNNGC	2 cut(s)	75, 669
CaiI	CAGNNNCTG	1 cut(s)	304
CciI	TCATGA	3 cut(s)	97, 535, 559
CfoI	GCGC	2 cut(s)	514, 547
Cfr13I	GGNCC	1 cut(s)	758
CsiI	ACCWGGT	1 cut(s)	213
Csp6I	GTAC	3 cut(s)	169, 236, 769
CviAII	CATG	5 cut(s)	98, 190, 536, 560, 611
CviQI	GTAC	3 cut(s)	169, 236, 769
DdeI	CTNAG	4 cut(s)	270, 297, 684, 752
DpnI	GATC	6 cut(s)	86, 128, 363, 540, 621, 720
DpnII	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
Eam1104I	CTCTTC	1 cut(s)	594
EarI	CTCTTC	1 cut(s)	594
Eco57I	CTGAAG	2 cut(s)	618, 647
EcoRII	CCWGG	2 cut(s)	213, 730
FaeI	CATG	5 cut(s)	101, 193, 539, 563, 614
FaiI	YATR	9 cut(s)	99, 115, 191, 261, 263, 537, 561, 612, 767
FatI	CATG	5 cut(s)	97, 189, 535, 559, 610
FauI	CCCGC	1 cut(s)	66
FbaI	TGATCA	3 cut(s)	84, 538, 718
Fnu4HI	GCNGC	7 cut(s)	346, 522, 525, 587, 680, 689, 757
FokI	GGATG	1 cut(s)	714
Fsp4HI	GCNGC	7 cut(s)	346, 522, 525, 587, 680, 689, 757
FspBI	CTAG	1 cut(s)	414
GlaI	GCGC	2 cut(s)	513, 546
GluI	GCNGC	7 cut(s)	346, 522, 525, 587, 680, 689, 757
GsuI	CTGGAG	3 cut(s)	407, 494, 599
HaeIII	GGCC	3 cut(s)	132, 735, 759
HhaI	GCGC	2 cut(s)	514, 547
Hin1II	CATG	5 cut(s)	101, 193, 539, 563, 614
Hin6I	GCGC	2 cut(s)	512, 545
HinP1I	GCGC	2 cut(s)	512, 545
HincII	GTYRAC	1 cut(s)	400
HindII	GTYRAC	1 cut(s)	400
HinfI	GANTC	5 cut(s)	94, 179, 229, 392, 574
HpaI	GTTAAC	1 cut(s)	400
HphI	GGTGA	2 cut(s)	650, 701
Hpy166II	GTNNAC	1 cut(s)	400
Hpy188I	TCNGA	3 cut(s)	504, 598, 753
Hpy188III	TCNNGA	8 cut(s)	88, 98, 305, 536, 551, 560, 578, 740
Hpy8I	GTNNAC	1 cut(s)	400
HpyAV	CCTTC	2 cut(s)	746, 771
HpyCH4V	TGCA	6 cut(s)	174, 319, 433, 458, 524, 679
HpyF10VI	GCNNNNNNNGC	6 cut(s)	79, 109, 330, 518, 685, 688
HpyF3I	CTNAG	4 cut(s)	270, 297, 684, 752
Hsp92II	CATG	5 cut(s)	101, 193, 539, 563, 614
HspAI	GCGC	2 cut(s)	512, 545
Ksp22I	TGATCA	3 cut(s)	84, 538, 718
KspAI	GTTAAC	1 cut(s)	400
Kzo9I	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
LguI	GCTCTTC	1 cut(s)	594
LmnI	GCTCC	2 cut(s)	196, 370
Lsp1109I	GCAGC	5 cut(s)	332, 508, 536, 573, 691
LweI	GCATC	6 cut(s)	109, 310, 523, 556, 573, 613
MabI	ACCWGGT	1 cut(s)	213
MaeI	CTAG	1 cut(s)	414
MaeIII	GTNAC	2 cut(s)	55, 217
MalI	GATC	6 cut(s)	86, 128, 363, 540, 621, 720
MbiI	CCGCTC	1 cut(s)	756
MboI	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
MboII	GAAGA	5 cut(s)	103, 443, 491, 611, 653
MhlI	GDGCHC	1 cut(s)	618
MluCI	AATT	2 cut(s)	308, 623
MlyI	GAGTC	3 cut(s)	88, 188, 401
MseI	TTAA	2 cut(s)	399, 507
MslI	CAYNNNNRTG	1 cut(s)	609
MspA1I	CMGCKG	1 cut(s)	385
MspR9I	CCNGG	2 cut(s)	215, 732
Mva1269I	GAATGC	1 cut(s)	271
MvaI	CCWGG	2 cut(s)	215, 732
MwoI	GCNNNNNNNGC	6 cut(s)	79, 109, 330, 518, 685, 688
NdeII	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
NlaIII	CATG	5 cut(s)	101, 193, 539, 563, 614
NmuCI	GTSAC	1 cut(s)	55
NspI	RCATGY	1 cut(s)	614
PagI	TCATGA	3 cut(s)	97, 535, 559
PaqCI	CACCTGC	1 cut(s)	556
PciI	ACATGT	1 cut(s)	610
PciSI	GCTCTTC	1 cut(s)	594
PcsI	WCGNNNNNNNCGW	1 cut(s)	349
PctI	GAATGC	1 cut(s)	271
PfeI	GAWTC	2 cut(s)	229, 574
PkrI	GCNGC	7 cut(s)	347, 523, 526, 588, 681, 690, 758
PleI	GAGTC	3 cut(s)	88, 187, 400
PpsI	GAGTC	3 cut(s)	88, 187, 400
PscI	ACATGT	1 cut(s)	610
Psp6I	CCWGG	2 cut(s)	213, 730
PspGI	CCWGG	2 cut(s)	213, 730
PspPI	GGNCC	1 cut(s)	758
PstI	CTGCAG	2 cut(s)	176, 526
PstNI	CAGNNNCTG	1 cut(s)	304
PvuII	CAGCTG	1 cut(s)	385
RsaI	GTAC	3 cut(s)	170, 237, 770
RsaNI	GTAC	3 cut(s)	169, 236, 769
RseI	CAYNNNNRTG	1 cut(s)	609
SapI	GCTCTTC	1 cut(s)	594
SaqAI	TTAA	2 cut(s)	399, 507
SatI	GCNGC	7 cut(s)	346, 522, 525, 587, 680, 689, 757
Sau3AI	GATC	6 cut(s)	84, 126, 361, 538, 619, 718
Sau96I	GGNCC	1 cut(s)	758
ScaI	AGTACT	1 cut(s)	170
SchI	GAGTC	3 cut(s)	88, 188, 401
ScrFI	CCNGG	2 cut(s)	215, 732
SduI	GDGCHC	1 cut(s)	618
SexAI	ACCWGGT	1 cut(s)	213
SfaNI	GCATC	6 cut(s)	109, 310, 523, 556, 573, 613
SfcI	CTRYAG	2 cut(s)	172, 522
SmiMI	CAYNNNNRTG	1 cut(s)	609
SmlI	CTYRAG	2 cut(s)	145, 551
SmoI	CTYRAG	2 cut(s)	145, 551
Sse9I	AATT	2 cut(s)	308, 623
SsiI	CCGC	4 cut(s)	73, 239, 689, 756
SspMI	CTAG	1 cut(s)	414
StyD4I	CCNGG	2 cut(s)	213, 730
TaqI	TCGA	7 cut(s)	26, 232, 352, 409, 607, 618, 741
TasI	AATT	2 cut(s)	308, 623
TatI	WGTACW	1 cut(s)	168
TauI	GCSGC	2 cut(s)	691, 759
TfiI	GAWTC	2 cut(s)	229, 574
Tru1I	TTAA	2 cut(s)	399, 507
Tru9I	TTAA	2 cut(s)	399, 507
TscAI	CASTG	2 cut(s)	157, 181
TseFI	GTSAC	1 cut(s)	55
TseI	GCWGC	5 cut(s)	345, 521, 524, 586, 679
Tsp45I	GTSAC	1 cut(s)	55
TspDTI	ATGAA	1 cut(s)	278
TspGWI	ACGGA	1 cut(s)	269
TspRI	CASTG	2 cut(s)	157, 181
XceI	RCATGY	1 cut(s)	614
XspI	CTAG	1 cut(s)	414
ZrmI	AGTACT	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.