Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011121

Physical Location & Seq

Genomic Coordinates Reverse (-)

38316327 .. 38324887

Gene Structure 8561 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1251500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3411 bp

ATGGAGTTTGAGGTCCGGTTAGTCGGAGGCATAGACAACTGCTTCGTCTCTCTCCCCCTGTCCCTAATCCAAACCCTCCACTCCTCCTCCCCATCCCTCCCGCCAGTTCTCGCTCTCGAGCTCCGCTCTTCCTCCACCGCCGACCGCTGGAACGTCGCTTGGTCCGGCGCCACCTCCGCTTCTCAGGCTATCGAGGTTGCTCAGCAATTTGGGGATTGCATTTCGTTGCCGGATCGCACTAGGGTTCAAGTTCGAGCTCTTTCGAATGTGCAGAAGGCTACTCTGGTCACTATTGAGCCTTCTACTGAGGATGATTGGGAGGTTTTGGAGCTTAATGCTGAGCTAGCAGAGGCTGCAATTTTGAATCAGGTGAGGATTCTCCATGAAGAAATGAGATTTCCTTTGTGGTTGAATGGCCGCACTTCCATTACATTCCAAGTTGCTTCAACCTTCCCGAAGAAAGCGGTGGTGCAACTGGTGCCAGGGACTGAAGTTGCAGTTGCTCCAAAGAGACGCAAGACTATCAACCCACATGGAGATCGTTCCATGCTCTCTTCTAATGGAAAGAATCATATTTCAAAGGCACTACTGCGCATTCAAGATCCGGACAGGAGATTAGTTCACAAAAGTTATGTCAAAGGCGTTGAGCTTGGAGTTGTTCTTACTTCTGTTGCTATGGTTCACCCAGAAACAGCAAAAATGTTCTCATTACAATCTCTTCAGTTCGTAGCTGTTGTGCCAAGATTATCACCAAAAGAGAGCATGAAAAATTCTGAAAATGATGGGTTGAAAACTAGAAGCAGTTCAACTCTGAAGGAATCTAACAGTGGAAATGACAAAAAGGACAATCGTGAAGTAATTGTTCGGCTGTTGATTTCTGATTCAGTTGCTAAAGGGCATGTTATGATTGCCCAATCTCTTCGTCTTTATTTGAGAACTCGTCTGCATTCATGGGTTTATTTGAAGGAATGCAATGGTATTTTGAAGAATGACATTCCATTCCTTTCACTTTCTCCTTGTCACTTTAAGATATTGAAGGACAAAGCTGTTGAAAGAAATGGTCTAGATGTTCTTGAAAGGCATAAACTTCGCAAGAAGAAAAACATGCTTCTGACGACCGGCTCAAGCACCTATATAGATGTTGCTGATTGGTCTACCCACGACAAAGTTGTTTCTGCTTTTTCATATGAAACTTCTTGCAAAGAAGATGAAGAGGCGGCTCCGCATTCTGACGAGTTAAAGGGAGTAGAGAGTCTTGTTAAAGTGTGGATCCTTGCACAATTTGATGCTATTGCTTCCAATGCAGGAGAAAAAGTTAATTCCTTGGTTTTGGGAAATGAAACTATACTTCACTTTGAAGTGAAGGTGCATAAGTTTGAGATTGAGGAAAAGCTACAAGAATCATCAGATGGATCCCTCGAAAACAAAAAGAAGAATGCTGAGCAACCAGTTGAAATCTTGTATGTACTGAAATTCTCCAATGAATCTCAACTTGCTGGAAATGCATATGAGCTTGTATTTGATGAAAGAAACAAAGGCAGTGATAATGTTGGAGGTGTAGAGTCAATTGGAAAGCTGAAGGTGGTCGATCCTAAGACGGCCGATCCTGTGACCTTTAATTCGGTTAGAGAGAGAATGTCTGAAAAAGATATCAATTCTGATGTATCAACCTTGAGCTGGATGGGGACAATTGCTTCTGACGTTTTAAATAGAATGTTGGTGTTGTTATCTCCTGCTTCTGGGGCATGGTTCAGCTCACACGATCTTCCGCTCCCTGGACATGTTCTGCTATATGGACCTCCTGGTTCGGGAAAGACATTATTGGCGAGAACTGTTGCTAAGTGTCTCGAAGAACATGAAAACCTCTTAGCGCACGTAGTTTTTGTACCTTGTTCTCAACTTGCCTTGGAAAAGGCCTCGGCCATTCGCCAAGCACTTTTGAGCTATATATCTGAGGCTTTAGATCATGCTCCATCTCTTGTAATTCTTGATGATCTTGATAGCATTGTTTCATCCTCTTCAGACTCCGAAGGATCTCAAGCTTCAACCTCTGTGCTTGCACTGACAGAGTTTCTCATTGACATTATGGATGAATATTGGGAAAAAAGGAAGAGCTCATGTGGAATTGGTCCGCTTGCATTCATAGCTTCAATAAAGTCTTTAGAGAGTATACCACAGTCATTAAGCTCTTCAGGAAGGTTTGACTTTCACGTGCAATTGCCTGCTCCTGCTGCCTCAGAACGTGCAGCCATTTTAAAGCATGAAATTCAGAGACGCTGCTTACAATGTTCTGATGATATCTTAGAAGATGTAGCTTCCAAATGTGATGGCTATGATTCATATGATCTGGAAATCTTGGTTGATAGAACTGTTCATGCTGCCATTGGTCGTTTTCTGCCTCATCATTTGTCCGTCGACAAGTGTGAAAAGCCCACTTTACTTAGGGATGATTTCTCTCGGGCAATGCATGATTTCCTTCCAGTTGCCATGCGCGACATCACTAAATCTGCTCCTGAAGGTGGGCGGTCTGGGTGGGATGATGTTGGCGGTCTTGTTGACATCCGCAATTCAATTAAAGAGATGATTGAATTGCCTTCAAGGTTTCCAAATATATTTGCCAAAGCTCCTTTAAGGTTGCGATCAAATATTCTATTGTATGGTCCTCCTGGCTGTGGAAAGACACACATAGTTGGTTCTGCTGCTGCGGCTTGTTCACTTAGATTTATATCCATCAAAGGACCTGAACTCTTGAATAAATACATTGGCGCTTCTGAGCAAGCTGTTCGGGATATATTCTCCAAAGCTGCTGCTGCAGCACCATGTCTTCTCTTTTTTGACGAATTTGATTCCATAGCCCCCAAAAGAGGGCATGACAATACTGGAGTAACTGATCGTGTTGTCAATCAGTTTCTGACCGAATTGGATGGTGTTGAAGTTTTGACTGGTGTTTTTGTTTTTGCTGCAACAAGTAGACCAGATTTACTTGATGCTGCACTGTTGAGACCTGGTAGGCTAGATCGCCTTCTGTTTTGTGATTTTCCATCTCTGCGTGAAAGGTTGGATATTTTGACGGTCCTTTCGAAAAAGCTACCAATGGATGACGATGTGGATTTAAGAGCCATAGCTTATATGACTGAGGGATTTAGTGGGGCCGACCTTCAAGCTCTGCTCTCAGATGCTCAGCTAGCAGCAGTGCATGAGATTCTAAACGCTGAAGATACCAGAGATCCAGGGAAGAAGCCAGTTATTTCTGATGCTCTCTTGAAGTCTACTGCTTCGAGGGCAAGACCATCTGTTTCCGAAGCTGAGAAGACAAGATTGTATGGCATTTACAACCAGTTCTTGGATTCAAAGAGATCTGTTGCTGCACAGTCGAGAGATGCAAAAGGCAAGAGGGCAACCCTAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0017038 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0042886 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0065002 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04146 map04146

Pfam Domains

Protein Families

AAA PEX-1N

Protein Analysis

1137

Amino Acids

124.89

Weight (kDa)

6.06

Isoelectric Point (pI)

46.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEX-1N	PF09262	96 - 169	3.9e-26	Peroxisome biogenesis factor 1, N-terminal
AAA_16	PF13191	591 - 692	1.8e-07	AAA ATPase domain
AAA	PF00004	595 - 741	8.1e-17	ATPase family associated with various cellular activities (AAA)
NACHT	PF05729	595 - 681	5.3e-06	NACHT domain
AAA	PF00004	883 - 1011	1.8e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	1035 - 1073	2.9e-12	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08470 AT5G08470 AT5G08470 AT5G08470
fragaria_vesca	FvH4_3g12310
malus_domestica	MD05G1251500.v1.1 MD15G1400900.v1.1
prunus_persica	Prupe.4G112300_v2.0.a1
pyrus_communis	pycom05g22920
rosa_chinensis	RchiOBHm_Chr5g0020121
rosa_laevigata	RLG00000032502
rosa_multiflora	Rmu_sc0000116.1_g000006
rosa_roxburghii	Rroxscaffold_1G00058080
rosa_rugosa	Rorug05G0057400 Rorug05G0057400
rosa_samantha	Rh5AG147300 Rh5BG146900 Rh5CG158300 Rh5DG146200
rosa_wichuraiana	Rw5G013020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	593
AccB1I	GGYRCC	2 cut(s)	167, 478
AccB7I	CCANNNNNTGG	1 cut(s)	3343
AccBSI	CCGCTC	2 cut(s)	126, 1771
AccI	GTMKAC	5 cut(s)	1154, 2170, 2415, 2971, 3269
AccII	CGCG	1 cut(s)	2493
AccIII	TCCGGA	1 cut(s)	604
AcoI	YGGCCR	3 cut(s)	415, 1599, 1920
AcsI	RAATTY	4 cut(s)	769, 1472, 2265, 2840
AcuI	CTGAAG	8 cut(s)	510, 704, 833, 1598, 2004, 2175, 2535, 3234
AcvI	CACGTG	1 cut(s)	2212
AcyI	GRCGYC	1 cut(s)	168
AfaI	GTAC	2 cut(s)	1467, 1887
AflIII	ACRYGT	1 cut(s)	1780
AjnI	CCWGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
Alw21I	GWGCWC	3 cut(s)	123, 259, 2117
Alw26I	GTCTC	5 cut(s)	52, 505, 1850, 2266, 2995
AlwNI	CAGNNNCTG	3 cut(s)	353, 488, 2911
Ama87I	CYCGRG	2 cut(s)	116, 2457
Aor13HI	TCCGGA	1 cut(s)	604
AoxI	GGCC	5 cut(s)	415, 1599, 1914, 1920, 3150
ApoI	RAATTY	4 cut(s)	769, 1472, 2265, 2840
ArsI	GACNNNNNNTTYG	8 cut(s)	26, 58, 618, 650, 1603, 1635, 3061, 3093
Asp700I	GAANNNNTTC	1 cut(s)	802
AspLEI	GCGC	5 cut(s)	170, 594, 1873, 2493, 2768
AspS9I	GGNCC	8 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073, 3150
AsuHPI	GGTGA	3 cut(s)	382, 674, 741
AsuII	TTCGAA	2 cut(s)	263, 3080
AsuNHI	GCTAGC	2 cut(s)	343, 3184
AvaI	CYCGRG	2 cut(s)	116, 2457
AvaII	GGWCC	7 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073
BamHI	GGATCC	2 cut(s)	1269, 1412
BanI	GGYRCC	2 cut(s)	167, 478
BanII	GRGCYC	3 cut(s)	123, 259, 2117
BbrPI	CACGTG	1 cut(s)	2212
BbsI	GAAGAC	2 cut(s)	2816, 3317
Bbv12I	GWGCWC	3 cut(s)	123, 259, 2117
BceAI	ACGGC	1 cut(s)	1614
BcgI	CGANNNNNNTGC	4 cut(s)	1070, 1104, 2765, 2799
BciT130I	CCWGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
BcoDI	GTCTC	5 cut(s)	52, 505, 1850, 2266, 2995
BfaI	CTAG	7 cut(s)	240, 344, 795, 1064, 3014, 3185, 3404
BfmI	CTRYAG	1 cut(s)	2811
BfoI	RGCGCY	2 cut(s)	171, 2769
BglII	AGATCT	1 cut(s)	3356
BlpI	GCTNAGC	4 cut(s)	201, 339, 1440, 3180
Bme1390I	CCNGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
Bme18I	GGWCC	7 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073
BmeT110I	CYCGRG	2 cut(s)	116, 2457
BmgT120I	GGNCC	8 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073, 3150
BmiI	GGNNCC	6 cut(s)	169, 480, 1221, 1271, 1414, 3151
BmrFI	CCNGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
BmsI	GCATC	5 cut(s)	1276, 2977, 3166, 3244, 3370
BmtI	GCTAGC	2 cut(s)	347, 3188
BpiI	GAAGAC	2 cut(s)	2816, 3317
BplI	GAGNNNNNCTC	2 cut(s)	110, 142
BpmI	CTGGAG	1 cut(s)	2901
Bpu1102I	GCTNAGC	4 cut(s)	201, 339, 1440, 3180
Bpu14I	TTCGAA	2 cut(s)	263, 3080
BpuEI	CTTGAG	3 cut(s)	1108, 1693, 2022
BsaAI	YACGTR	2 cut(s)	1876, 2212
BsaBI	GATNNNNATC	1 cut(s)	2725
BsaHI	GRCGYC	1 cut(s)	168
BsaI	GGTCTC	1 cut(s)	2995
BsaJI	CCNNGG	6 cut(s)	482, 1323, 1774, 1905, 1917, 3230
BsaWI	WCCGGW	2 cut(s)	15, 604
BsaXI	ACNNNNNCTCC	4 cut(s)	71, 101, 1299, 1329
Bse118I	RCCGGY	1 cut(s)	1118
Bse1I	ACTGG	8 cut(s)	104, 480, 1448, 2480, 2884, 2947, 3242, 3337
Bse3DI	GCAATG	2 cut(s)	979, 2469
Bse8I	GATNNNNATC	1 cut(s)	2725
BseAI	TCCGGA	1 cut(s)	604
BseBI	CCWGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
BseDI	CCNNGG	6 cut(s)	482, 1323, 1774, 1905, 1917, 3230
BseJI	GATNNNNATC	1 cut(s)	2725
BseMI	GCAATG	2 cut(s)	979, 2469
BseNI	ACTGG	8 cut(s)	104, 480, 1448, 2480, 2884, 2947, 3242, 3337
BseRI	GAGGAG	2 cut(s)	73, 76
BseX3I	CGGCCG	1 cut(s)	1599
BsgI	GTGCAG	4 cut(s)	290, 2265, 2976, 3351
Bsh1236I	CGCG	1 cut(s)	2493
Bsh1285I	CGRYCG	3 cut(s)	145, 1119, 1602
BshFI	GGCC	5 cut(s)	417, 1601, 1916, 1922, 3152
BshNI	GGYRCC	2 cut(s)	167, 478
BsiEI	CGRYCG	3 cut(s)	145, 1119, 1602
BsiHKAI	GWGCWC	3 cut(s)	123, 259, 2117
BsiHKCI	CYCGRG	2 cut(s)	116, 2457
BsiSI	CCGG	5 cut(s)	16, 165, 230, 605, 1119
BslFI	GGGAC	3 cut(s)	46, 499, 1699
BsmAI	GTCTC	5 cut(s)	52, 505, 1850, 2266, 2995
BsmBI	CGTCTC	3 cut(s)	52, 505, 2266
BsmFI	GGGAC	3 cut(s)	46, 499, 1699
BsmI	GAATGC	6 cut(s)	594, 946, 974, 1225, 1441, 2138
BsnI	GGCC	5 cut(s)	417, 1601, 1916, 1922, 3152
Bso31I	GGTCTC	1 cut(s)	2995
BsoBI	CYCGRG	2 cut(s)	116, 2457
Bsp119I	TTCGAA	2 cut(s)	263, 3080
Bsp1286I	GDGCHC	3 cut(s)	123, 259, 2117
Bsp13I	TCCGGA	1 cut(s)	604
Bsp1720I	GCTNAGC	4 cut(s)	201, 339, 1440, 3180
BspANI	GGCC	5 cut(s)	417, 1601, 1916, 1922, 3152
BspEI	TCCGGA	1 cut(s)	604
BspFNI	CGCG	1 cut(s)	2493
BspLI	GGNNCC	6 cut(s)	169, 480, 1221, 1271, 1414, 3151
BspMAI	CTGCAG	1 cut(s)	2815
BspOI	GCTAGC	2 cut(s)	347, 3188
BspQI	GCTCTTC	3 cut(s)	133, 2105, 2194
BspT104I	TTCGAA	2 cut(s)	263, 3080
BspT107I	GGYRCC	2 cut(s)	167, 478
BspTNI	GGTCTC	1 cut(s)	2995
BsrBI	CCGCTC	2 cut(s)	126, 1771
BsrDI	GCAATG	2 cut(s)	979, 2469
BsrFI	RCCGGY	1 cut(s)	1118
BsrI	ACTGG	8 cut(s)	104, 480, 1448, 2480, 2884, 2947, 3242, 3337
BssAI	RCCGGY	1 cut(s)	1118
BssECI	CCNNGG	6 cut(s)	482, 1323, 1774, 1905, 1917, 3230
BssNAI	GTATAC	1 cut(s)	2171
BssNI	GRCGYC	1 cut(s)	168
BssT1I	CCWWGG	2 cut(s)	1323, 1905
Bst1107I	GTATAC	1 cut(s)	2171
Bst2UI	CCWGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
Bst4CI	ACNGT	7 cut(s)	827, 1834, 2178, 2371, 2997, 3073, 3372
Bst6I	CTCTTC	8 cut(s)	133, 559, 723, 924, 1206, 2023, 2105, 2194
BstACI	GRCGYC	1 cut(s)	168
BstAPI	GCANNNNNTGC	2 cut(s)	353, 478
BstBAI	YACGTR	2 cut(s)	1876, 2212
BstBI	TTCGAA	2 cut(s)	263, 3080
BstC8I	GCNNGC	6 cut(s)	345, 2058, 2136, 2223, 2778, 3186
BstFNI	CGCG	1 cut(s)	2493
BstH2I	RGCGCY	2 cut(s)	171, 2769
BstHHI	GCGC	5 cut(s)	170, 594, 1873, 2493, 2768
BstMAI	GTCTC	5 cut(s)	52, 505, 1850, 2266, 2995
BstMCI	CGRYCG	3 cut(s)	145, 1119, 1602
BstNI	CCWGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
BstNSI	RCATGY	3 cut(s)	902, 1108, 1784
BstSCI	CCNGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
BstSFI	CTRYAG	1 cut(s)	2811
BstUI	CGCG	1 cut(s)	2493
BstV2I	GAAGAC	2 cut(s)	2816, 3317
BstX2I	RGATCY	6 cut(s)	601, 1269, 1412, 2033, 3226, 3356
BstYI	RGATCY	6 cut(s)	601, 1269, 1412, 2033, 3226, 3356
BstZ17I	GTATAC	1 cut(s)	2171
BstZI	CGGCCG	1 cut(s)	1599
BsuRI	GGCC	5 cut(s)	417, 1601, 1916, 1922, 3152
BtsI	GCAGTG	2 cut(s)	1546, 3198
BtsIMutI	CAGTG	5 cut(s)	832, 1546, 2060, 2993, 3198
Cac8I	GCNNGC	6 cut(s)	345, 2058, 2136, 2223, 2778, 3186
CaiI	CAGNNNCTG	3 cut(s)	353, 488, 2911
CfoI	GCGC	5 cut(s)	170, 594, 1873, 2493, 2768
Cfr10I	RCCGGY	1 cut(s)	1118
Cfr13I	GGNCC	8 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073, 3150
CseI	GACGC	2 cut(s)	522, 2283
CsiI	ACCWGGT	1 cut(s)	3004
Csp6I	GTAC	2 cut(s)	1466, 1886
CviQI	GTAC	2 cut(s)	1466, 1886
DinI	GGCGCC	1 cut(s)	169
DraI	TTTAAA	2 cut(s)	1707, 2256
EaeI	YGGCCR	3 cut(s)	415, 1599, 1920
EagI	CGGCCG	1 cut(s)	1599
Eam1104I	CTCTTC	8 cut(s)	133, 559, 723, 924, 1206, 2023, 2105, 2194
EarI	CTCTTC	8 cut(s)	133, 559, 723, 924, 1206, 2023, 2105, 2194
Ecl136II	GAGCTC	3 cut(s)	121, 257, 2115
EclXI	CGGCCG	1 cut(s)	1599
Eco130I	CCWWGG	2 cut(s)	1323, 1905
Eco147I	AGGCCT	1 cut(s)	1916
Eco24I	GRGCYC	3 cut(s)	123, 259, 2117
Eco31I	GGTCTC	1 cut(s)	2995
Eco32I	GATATC	2 cut(s)	1651, 2299
Eco47I	GGWCC	7 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073
Eco52I	CGGCCG	1 cut(s)	1599
Eco53kI	GAGCTC	3 cut(s)	121, 257, 2115
Eco57I	CTGAAG	8 cut(s)	510, 704, 833, 1598, 2004, 2175, 2535, 3234
Eco72I	CACGTG	1 cut(s)	2212
Eco88I	CYCGRG	2 cut(s)	116, 2457
EcoICRI	GAGCTC	3 cut(s)	121, 257, 2115
EcoO109I	RGGNCCY	1 cut(s)	2738
EcoRII	CCWGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
EcoRV	GATATC	2 cut(s)	1651, 2299
EcoT14I	CCWWGG	2 cut(s)	1323, 1905
EcoT22I	ATGCAT	2 cut(s)	1507, 2469
EcoT38I	GRGCYC	3 cut(s)	123, 259, 2117
EgeI	GGCGCC	1 cut(s)	169
EheI	GGCGCC	1 cut(s)	169
ErhI	CCWWGG	2 cut(s)	1323, 1905
Esp3I	CGTCTC	3 cut(s)	52, 505, 2266
FaqI	GGGAC	3 cut(s)	46, 499, 1699
FauI	CCCGC	1 cut(s)	108
FauNDI	CATATG	3 cut(s)	1186, 1507, 2341
FblI	GTMKAC	5 cut(s)	1154, 2170, 2415, 2971, 3269
FriOI	GRGCYC	3 cut(s)	123, 259, 2117
FspBI	CTAG	7 cut(s)	240, 344, 795, 1064, 3014, 3185, 3404
FspI	TGCGCA	1 cut(s)	593
GlaI	GCGC	5 cut(s)	169, 593, 1872, 2492, 2767
GsuI	CTGGAG	1 cut(s)	2901
HaeII	RGCGCY	2 cut(s)	171, 2769
HaeIII	GGCC	5 cut(s)	417, 1601, 1916, 1922, 3152
HapII	CCGG	5 cut(s)	16, 165, 230, 605, 1119
HgaI	GACGC	2 cut(s)	522, 2283
HhaI	GCGC	5 cut(s)	170, 594, 1873, 2493, 2768
Hin1I	GRCGYC	1 cut(s)	168
Hin6I	GCGC	5 cut(s)	168, 592, 1871, 2491, 2766
HinP1I	GCGC	5 cut(s)	168, 592, 1871, 2491, 2766
HincII	GTYRAC	2 cut(s)	2416, 2557
HindII	GTYRAC	2 cut(s)	2416, 2557
HindIII	AAGCTT	1 cut(s)	2040
HpaII	CCGG	5 cut(s)	16, 165, 230, 605, 1119
HphI	GGTGA	3 cut(s)	382, 674, 741
Hpy166II	GTNNAC	9 cut(s)	622, 682, 1155, 2171, 2416, 2557, 2714, 2972, 3270
Hpy8I	GTNNAC	9 cut(s)	622, 682, 1155, 2171, 2416, 2557, 2714, 2972, 3270
Hpy99I	CGWCG	2 cut(s)	158, 2417
HpyCH4III	ACNGT	7 cut(s)	827, 1834, 2178, 2371, 2997, 3073, 3372
HpyCH4IV	ACGT	5 cut(s)	153, 1701, 1875, 2211, 2242
HpySE526I	ACGT	5 cut(s)	153, 1701, 1875, 2211, 2242
Hsp92I	GRCGYC	1 cut(s)	168
HspAI	GCGC	5 cut(s)	168, 592, 1871, 2491, 2766
KasI	GGCGCC	1 cut(s)	167
Kpn2I	TCCGGA	1 cut(s)	604
LguI	GCTCTTC	3 cut(s)	133, 2105, 2194
LmnI	GCTCC	9 cut(s)	126, 328, 508, 1225, 1776, 1975, 2230, 2515, 2629
LweI	GCATC	5 cut(s)	1276, 2977, 3166, 3244, 3370
MabI	ACCWGGT	1 cut(s)	3004
MaeI	CTAG	7 cut(s)	240, 344, 795, 1064, 3014, 3185, 3404
MaeII	ACGT	5 cut(s)	153, 1701, 1875, 2211, 2242
MaeIII	GTNAC	4 cut(s)	286, 1019, 1609, 2884
MbiI	CCGCTC	2 cut(s)	126, 1771
MfeI	CAATTG	3 cut(s)	1566, 1689, 2216
MflI	RGATCY	6 cut(s)	601, 1269, 1412, 2033, 3226, 3356
MhlI	GDGCHC	3 cut(s)	123, 259, 2117
Mly113I	GGCGCC	1 cut(s)	168
MlyI	GAGTC	3 cut(s)	1261, 1571, 2018
MmeI	TCCRAC	3 cut(s)	4, 1531, 3039
Mph1103I	ATGCAT	2 cut(s)	1507, 2469
MroI	TCCGGA	1 cut(s)	604
MroXI	GAANNNNTTC	1 cut(s)	802
MspA1I	CMGCKG	1 cut(s)	147
MspI	CCGG	5 cut(s)	16, 165, 230, 605, 1119
MspR9I	CCNGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
MunI	CAATTG	3 cut(s)	1566, 1689, 2216
Mva1269I	GAATGC	6 cut(s)	594, 946, 974, 1225, 1441, 2138
MvaI	CCWGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
MvnI	CGCG	1 cut(s)	2493
NarI	GGCGCC	1 cut(s)	168
NdeI	CATATG	3 cut(s)	1186, 1507, 2341
NheI	GCTAGC	2 cut(s)	343, 3184
NlaIV	GGNNCC	6 cut(s)	169, 480, 1221, 1271, 1414, 3151
NmeAIII	GCCGAG	1 cut(s)	1898
NmuCI	GTSAC	3 cut(s)	286, 1019, 1609
NsbI	TGCGCA	1 cut(s)	593
NsiI	ATGCAT	2 cut(s)	1507, 2469
NspI	RCATGY	3 cut(s)	902, 1108, 1784
NspV	TTCGAA	2 cut(s)	263, 3080
PaeR7I	CTCGAG	1 cut(s)	116
PceI	AGGCCT	1 cut(s)	1916
PciI	ACATGT	1 cut(s)	1780
PciSI	GCTCTTC	3 cut(s)	133, 2105, 2194
PcsI	WCGNNNNNNNCGW	1 cut(s)	3077
PctI	GAATGC	6 cut(s)	594, 946, 974, 1225, 1441, 2138
PdmI	GAANNNNTTC	1 cut(s)	802
PflMI	CCANNNNNTGG	1 cut(s)	3343
PleI	GAGTC	3 cut(s)	1260, 1570, 2018
PluTI	GGCGCC	1 cut(s)	171
PmaCI	CACGTG	1 cut(s)	2212
PmlI	CACGTG	1 cut(s)	2212
PpsI	GAGTC	3 cut(s)	1260, 1570, 2018
Ppu21I	YACGTR	2 cut(s)	1876, 2212
PpuMI	RGGWCCY	1 cut(s)	2738
PscI	ACATGT	1 cut(s)	1780
Psp124BI	GAGCTC	3 cut(s)	123, 259, 2117
Psp5II	RGGWCCY	1 cut(s)	2738
Psp6I	CCWGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
PspCI	CACGTG	1 cut(s)	2212
PspGI	CCWGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
PspN4I	GGNNCC	6 cut(s)	169, 480, 1221, 1271, 1414, 3151
PspPI	GGNCC	8 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073, 3150
PspPPI	RGGWCCY	1 cut(s)	2738
PstI	CTGCAG	1 cut(s)	2815
PstNI	CAGNNNCTG	3 cut(s)	353, 488, 2911
PsuI	RGATCY	6 cut(s)	601, 1269, 1412, 2033, 3226, 3356
RsaI	GTAC	2 cut(s)	1467, 1887
RsaNI	GTAC	2 cut(s)	1466, 1886
SacI	GAGCTC	3 cut(s)	123, 259, 2117
SalI	GTCGAC	1 cut(s)	2414
SapI	GCTCTTC	3 cut(s)	133, 2105, 2194
Sau96I	GGNCC	8 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073, 3150
SchI	GAGTC	3 cut(s)	1261, 1571, 2018
ScrFI	CCNGG	6 cut(s)	483, 1776, 1803, 2667, 3006, 3231
SduI	GDGCHC	3 cut(s)	123, 259, 2117
SexAI	ACCWGGT	1 cut(s)	3004
SfaNI	GCATC	5 cut(s)	1276, 2977, 3166, 3244, 3370
SfcI	CTRYAG	1 cut(s)	2811
SfoI	GGCGCC	1 cut(s)	169
Sfr274I	CTCGAG	1 cut(s)	116
SfuI	TTCGAA	2 cut(s)	263, 3080
SinI	GGWCC	7 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073
SlaI	CTCGAG	1 cut(s)	116
SmlI	CTYRAG	4 cut(s)	116, 1123, 1672, 2037
SmoI	CTYRAG	4 cut(s)	116, 1123, 1672, 2037
SseBI	AGGCCT	1 cut(s)	1916
SspDI	GGCGCC	1 cut(s)	167
SspI	AATATT	2 cut(s)	2096, 2647
SspMI	CTAG	7 cut(s)	240, 344, 795, 1064, 3014, 3185, 3404
SstI	GAGCTC	3 cut(s)	123, 259, 2117
StuI	AGGCCT	1 cut(s)	1916
StyD4I	CCNGG	6 cut(s)	481, 1774, 1801, 2665, 3004, 3229
StyI	CCWWGG	2 cut(s)	1323, 1905
TaaI	ACNGT	7 cut(s)	827, 1834, 2178, 2371, 2997, 3073, 3372
TaiI	ACGT	5 cut(s)	156, 1704, 1878, 2214, 2245
TaqII	GACCGA	1 cut(s)	2930
TatI	WGTACW	1 cut(s)	1465
TauI	GCSGC	3 cut(s)	420, 1220, 2708
TscAI	CASTG	5 cut(s)	832, 1546, 2067, 3000, 3198
TseFI	GTSAC	3 cut(s)	286, 1019, 1609
Tsp45I	GTSAC	3 cut(s)	286, 1019, 1609
TspGWI	ACGGA	1 cut(s)	2401
TspRI	CASTG	5 cut(s)	832, 1546, 2067, 3000, 3198
Van91I	CCANNNNNTGG	1 cut(s)	3343
VpaK11BI	GGWCC	7 cut(s)	13, 162, 1796, 2129, 2660, 2738, 3073
XapI	RAATTY	4 cut(s)	769, 1472, 2265, 2840
XbaI	TCTAGA	1 cut(s)	1063
XceI	RCATGY	3 cut(s)	902, 1108, 1784
XhoI	CTCGAG	1 cut(s)	116
XmiI	GTMKAC	5 cut(s)	1154, 2170, 2415, 2971, 3269
XmnI	GAANNNNTTC	1 cut(s)	802
XspI	CTAG	7 cut(s)	240, 344, 795, 1064, 3014, 3185, 3404
Zsp2I	ATGCAT	2 cut(s)	1507, 2469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.