MD05G1262400.v1.1

Belongs to the SNF7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015854
Physical Location & Seq
Forward (+)
39735939 .. 39740583
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262400.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCGGATACGTGGGAACCGGGAACAAAGCGCACGCTCCCCTATAAACCAGATTCAGAAGACTTTGCCTTATTGGTTTGTTTGGACTGTCAAACTCTCGCAACTCTCCAAAAGTTCCCAACTTCACAACTCTACTGGGTTCTCACTCACTCAGTCCACCATGAACATCTTCAAGAAGAAGACCTCGCCCAAAGAGGCTCTCAGGGAAAGCAAAAGAGAAATGAGTGTTGCTACAAGAGGTATTGAACGTGAGATCGCATCTCTTCAGTTGGAGGAGAGGAAATTGGTGGCGGAGATTAAGAAAACGGCTAAAACTGGAAACGAGGCTGCCACAAAAATCCTGGCTCGTCAGCTCGTTAGGCTACGCCAACAGATTACTAACTTGCAAGGAAGTCGTGCCCAAATAAGAGGTGTAGGTACCCATACACAGGCATTATACGCAAGCACTTCTATTTCCACAGGCATGAAAGGTGCAACTAAAGCAATGGTGGCAATGAACAAGCAAATGGCACCTGCAAAACAAGTCAAAGTGATTAGAGAATTCCAGAAGAACGCAGCACAAATGGATATGACGATAGAAATGATGTCAGAGTCTATTGATGAAACCTTGGACAAAGATGAGGCTGAAGAGGAAACAGAGGAGCTCACTAACCAGGTGCTCGATGAGATTGGTGTTGATATTGCATCCCAGTTATCTTCAGCTCCAAAAGGCAGAATTGCATCAAGAAATACTGTAAATGTAGCTCCGAGGAATGCTGAAAATGTGGCTTCAAGGAATGCTGAAAATGTAGCTCCAAAGAATGCTGCAAATGTTGTTAACAGTTCTGAATCGCCCGACGTTGAGGATCTTGAGAAGAGGTTGGCCTCTCTGCGACGTTTGTGA

Protein Analysis

294

Amino Acids

32.54

Weight (kDa)

9.81

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 69 - 238 6.6e-37 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44560 AT5G44560
fragaria_vesca FvH4_3g11600
malus_domestica MD05G1262400.v1.1 MD10G1240400.v1.1
prunus_persica Prupe.4G103400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0018561
rosa_laevigata RLG00000032379
rosa_multiflora Rmu_sc0002579.1_g000007 Rmu_sc0007192.1_g000012
rosa_roxburghii Rroxscaffold_1G00059190
rosa_rugosa Rorug05G0045200
rosa_samantha Rh5AG136200 Rh5BG135200
rosa_wichuraiana Rw5G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 2 cut(s) 416, 508
AciI CCGC 2 cut(s) 4, 290
AclWI GGATC 1 cut(s) 852
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 3 cut(s) 248, 645, 681
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 3 cut(s) 172, 245, 771
AjnI CCWGG 2 cut(s) 339, 651
AluBI AGCT 5 cut(s) 352, 643, 701, 743, 791
AluI AGCT 5 cut(s) 352, 643, 701, 743, 791
Alw21I GWGCWC 2 cut(s) 645, 660
AlwI GGATC 1 cut(s) 852
AoxI GGCC 1 cut(s) 861
ApeKI GCWGC 3 cut(s) 326, 554, 803
ApoI RAATTY 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 167
Asp718I GGTACC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 400
BanI GGYRCC 2 cut(s) 416, 508
BanII GRGCYC 1 cut(s) 645
BbsI GAAGAC 2 cut(s) 65, 185
Bbv12I GWGCWC 2 cut(s) 645, 660
BbvI GCAGC 3 cut(s) 313, 566, 790
BceAI ACGGC 1 cut(s) 321
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 2 cut(s) 341, 653
BcnI CCSGG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 3 cut(s) 327, 555, 804
BlsI GCNGC 3 cut(s) 328, 556, 805
Bme1390I CCNGG 3 cut(s) 20, 341, 653
BmiI GGNNCC 3 cut(s) 17, 418, 510
BmrFI CCNGG 3 cut(s) 20, 341, 653
BmrI ACTGGG 2 cut(s) 144, 682
BmsI GCATC 3 cut(s) 266, 692, 728
BmuI ACTGGG 2 cut(s) 144, 682
BpiI GAAGAC 2 cut(s) 65, 185
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 606, 746
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 3 cut(s) 139, 319, 688
Bse3DI GCAATG 2 cut(s) 489, 498
BseBI CCWGG 2 cut(s) 341, 653
BseDI CCNNGG 2 cut(s) 606, 746
BseGI GGATG 1 cut(s) 683
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 2 cut(s) 489, 498
BseMII CTCAG 2 cut(s) 164, 214
BseNI ACTGG 3 cut(s) 139, 319, 688
BseRI GAGGAG 2 cut(s) 287, 653
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 3 cut(s) 313, 566, 790
BshFI GGCC 1 cut(s) 863
BshNI GGYRCC 2 cut(s) 416, 508
BsiHKAI GWGCWC 2 cut(s) 645, 660
BsiSI CCGG 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 427
BsmI GAATGC 3 cut(s) 757, 781, 805
BsnI GGCC 1 cut(s) 863
Bsp1286I GDGCHC 3 cut(s) 400, 645, 660
Bsp143I GATC 2 cut(s) 252, 844
BspACI CCGC 2 cut(s) 4, 290
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 2 cut(s) 163, 213
BspLI GGNNCC 3 cut(s) 17, 418, 510
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 852
BspT107I GGYRCC 2 cut(s) 416, 508
BsrDI GCAATG 2 cut(s) 489, 498
BsrI ACTGG 3 cut(s) 139, 319, 688
BssECI CCNNGG 2 cut(s) 606, 746
BssMI GATC 2 cut(s) 252, 844
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 2 cut(s) 341, 653
Bst4CI ACNGT 3 cut(s) 88, 733, 821
Bst6I CTCTTC 3 cut(s) 267, 621, 848
BstBAI YACGTR 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 34, 442
BstDEI CTNAG 2 cut(s) 150, 200
BstF5I GGATG 1 cut(s) 683
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 2 cut(s) 255, 847
BstMBI GATC 2 cut(s) 252, 844
BstMWI GCNNNNNNNGC 4 cut(s) 358, 437, 479, 488
BstNI CCWGG 2 cut(s) 341, 653
BstSCI CCNGG 3 cut(s) 18, 339, 651
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 3 cut(s) 313, 566, 790
BstV2I GAAGAC 2 cut(s) 65, 185
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
BsuRI GGCC 1 cut(s) 863
BtsCI GGATG 1 cut(s) 683
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 34, 442
CfoI GCGC 1 cut(s) 32
CsiI ACCWGGT 1 cut(s) 651
Csp6I GTAC 1 cut(s) 417
CviAII CATG 2 cut(s) 160, 463
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 2 cut(s) 150, 200
DpnI GATC 2 cut(s) 254, 846
DpnII GATC 2 cut(s) 252, 844
Eam1104I CTCTTC 3 cut(s) 267, 621, 848
EarI CTCTTC 3 cut(s) 267, 621, 848
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 643
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 643
Eco57I CTGAAG 3 cut(s) 248, 645, 681
EcoICRI GAGCTC 1 cut(s) 643
EcoRI GAATTC 1 cut(s) 539
EcoRII CCWGG 2 cut(s) 339, 651
EcoT14I CCWWGG 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 2 cut(s) 163, 466
FaiI YATR 6 cut(s) 44, 161, 423, 436, 464, 569
FatI CATG 2 cut(s) 159, 462
Fnu4HI GCNGC 3 cut(s) 327, 555, 804
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 3 cut(s) 327, 555, 804
GlaI GCGC 1 cut(s) 31
GluI GCNGC 3 cut(s) 327, 555, 804
HaeIII GGCC 1 cut(s) 863
HapII CCGG 1 cut(s) 19
HhaI GCGC 1 cut(s) 32
Hin1II CATG 2 cut(s) 163, 466
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 3 cut(s) 52, 590, 827
HpaI GTTAAC 1 cut(s) 817
HpaII CCGG 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 156, 817
Hpy188I TCNGA 4 cut(s) 57, 589, 747, 826
Hpy188III TCNNGA 4 cut(s) 172, 544, 723, 848
Hpy8I GTNNAC 2 cut(s) 156, 817
Hpy99I CGWCG 2 cut(s) 839, 876
HpyCH4III ACNGT 3 cut(s) 88, 733, 821
HpyCH4IV ACGT 4 cut(s) 10, 247, 837, 874
HpyCH4V TGCA 6 cut(s) 385, 473, 515, 683, 719, 806
HpyF10VI GCNNNNNNNGC 4 cut(s) 358, 437, 479, 488
HpyF3I CTNAG 2 cut(s) 150, 200
HpySE526I ACGT 4 cut(s) 10, 247, 837, 874
Hsp92II CATG 2 cut(s) 163, 466
HspAI GCGC 1 cut(s) 30
KpnI GGTACC 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 2 cut(s) 252, 844
LmnI GCTCC 5 cut(s) 41, 640, 706, 748, 796
Lsp1109I GCAGC 3 cut(s) 313, 566, 790
LweI GCATC 3 cut(s) 266, 692, 728
MabI ACCWGGT 1 cut(s) 651
MaeII ACGT 4 cut(s) 10, 247, 837, 874
MalI GATC 2 cut(s) 254, 846
MboI GATC 2 cut(s) 252, 844
MboII GAAGA 9 cut(s) 70, 160, 187, 190, 254, 559, 638, 687, 865
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 3 cut(s) 400, 645, 660
MluCI AATT 3 cut(s) 281, 539, 714
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 2 cut(s) 297, 816
MslI CAYNNNNRTG 1 cut(s) 461
MspI CCGG 1 cut(s) 19
MspR9I CCNGG 3 cut(s) 20, 341, 653
Mva1269I GAATGC 3 cut(s) 757, 781, 805
MvaI CCWGG 2 cut(s) 341, 653
MwoI GCNNNNNNNGC 4 cut(s) 358, 437, 479, 488
NciI CCSGG 1 cut(s) 20
NdeII GATC 2 cut(s) 252, 844
NlaIII CATG 2 cut(s) 163, 466
NlaIV GGNNCC 3 cut(s) 17, 418, 510
PaqCI CACCTGC 1 cut(s) 520
PctI GAATGC 3 cut(s) 757, 781, 805
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 52, 827
PkrI GCNGC 3 cut(s) 328, 556, 805
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Ppu21I YACGTR 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 645
Psp6I CCWGG 2 cut(s) 339, 651
PspGI CCWGG 2 cut(s) 339, 651
PspN4I GGNNCC 3 cut(s) 17, 418, 510
PsuI RGATCY 1 cut(s) 844
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 1 cut(s) 645
SaqAI TTAA 2 cut(s) 297, 816
SatI GCNGC 3 cut(s) 327, 555, 804
Sau3AI GATC 2 cut(s) 252, 844
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 20, 341, 653
SduI GDGCHC 3 cut(s) 400, 645, 660
SexAI ACCWGGT 1 cut(s) 651
SfaNI GCATC 3 cut(s) 266, 692, 728
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
Sse9I AATT 3 cut(s) 281, 539, 714
SsiI CCGC 2 cut(s) 4, 290
SstI GAGCTC 1 cut(s) 645
StyD4I CCNGG 3 cut(s) 18, 339, 651
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 88, 733, 821
TaiI ACGT 4 cut(s) 13, 250, 840, 877
TaqI TCGA 1 cut(s) 660
TasI AATT 3 cut(s) 281, 539, 714
TfiI GAWTC 2 cut(s) 52, 827
Tru1I TTAA 2 cut(s) 297, 816
Tru9I TTAA 2 cut(s) 297, 816
TseI GCWGC 3 cut(s) 326, 554, 803
TspDTI ATGAA 4 cut(s) 176, 479, 509, 615
XapI RAATTY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.