MD10G1240400.v1.1

Belongs to the SNF7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015854
Physical Location & Seq
Forward (+)
33595520 .. 33599922
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1240400.v1.1.491

Sequence Viewer

Length: 750 bp
ATGAACATCTTCAAGAAGAAGACCTCCCCCAAAGAGGCTCTCAGGGAAAGCAAAAGAGAAATGAGTGTTGCTACAAGAGGTATTGAACGTGAGATCGCATCTCTTCAGTTGGAGGAGAGGAAATTAGTGGCGGAGATCAAGAAAACGGCTAAAACTGGTAACGAGGCTGCCACCAAAATCCTTGCTCGTCAACTCGTTAGACTACGTCAACAGATTACTAACTTGCAAGGAAGTCGTGCCCAAATAAGAGGTGTAGCTACCCATACACAGGCATTGTATGCAAGCACTTCTATTTCCACAGGCATGAAAGGTGCAACTAAAGCAATGGTGGCAATGAACAAGCAAATGGAACCTGCAAAACAAGTTAAAGTGATCAGAGAATTCCAGAAGCAGGCAGCACAAATGGATATGACGATAGAAATGATGTCAGAGTCTATTGATGAAACCTTGGACAAAGATGAGGCTGAAGAGGAAACAGAGGAGCTCACCAACCAGGTGCTCGATGAGATTGGTGTTGATATTGCATCGCAGTTATCTTCAGCTCCAAAAGGCAGAATTGCATCGAGAACTACTGTAAATGTAGCTCCGAGGAATGCTGAAAATGTCGCTTCAAGGAATGCTGCTGAAAATGTAGCTTCAAGGAATGCTGAAAATGTAGCTCCAAAGAATGCTGCAAATGTTGTTAACAGTTCTGAATCATCTGACGTTGAGGATCTTGAGAAGAGGTTGGCCTCTCTGCGACGTTTGTGA

Protein Analysis

250

Amino Acids

27.4

Weight (kDa)

9.22

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 16 - 185 6.9e-38 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44560 AT5G44560
fragaria_vesca FvH4_3g11600
malus_domestica MD05G1262400.v1.1 MD10G1240400.v1.1
prunus_persica Prupe.4G103400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0018561
rosa_laevigata RLG00000032379
rosa_multiflora Rmu_sc0002579.1_g000007 Rmu_sc0007192.1_g000012
rosa_roxburghii Rroxscaffold_1G00059190
rosa_rugosa Rorug05G0045200
rosa_samantha Rh5AG136200 Rh5BG135200
rosa_wichuraiana Rw5G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
AciI CCGC 1 cut(s) 131
AclWI GGATC 1 cut(s) 720
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 3 cut(s) 89, 486, 522
AfiI CCNNNNNNNGG 3 cut(s) 34, 268, 391
AgsI TTSAA 4 cut(s) 13, 86, 612, 639
AjnI CCWGG 1 cut(s) 492
AluBI AGCT 6 cut(s) 257, 484, 542, 584, 635, 659
AluI AGCT 6 cut(s) 257, 484, 542, 584, 635, 659
Alw21I GWGCWC 2 cut(s) 486, 501
AlwI GGATC 1 cut(s) 720
AoxI GGCC 1 cut(s) 729
ApeKI GCWGC 4 cut(s) 167, 395, 620, 671
ApoI RAATTY 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 486, 501
BbvI GCAGC 4 cut(s) 154, 407, 607, 658
BceAI ACGGC 1 cut(s) 162
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 494
BclI TGATCA 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 4 cut(s) 168, 396, 621, 672
BlsI GCNGC 4 cut(s) 169, 397, 622, 673
Bme1390I CCNGG 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 107, 533, 569
BpiI GAAGAC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 737
BsaJI CCNNGG 2 cut(s) 447, 587
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 268, 391
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 2 cut(s) 330, 339
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 447, 587
BseLI CCNNNNNNNGG 3 cut(s) 34, 268, 391
BseMI GCAATG 2 cut(s) 330, 339
BseMII CTCAG 1 cut(s) 55
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 128, 494
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 4 cut(s) 154, 407, 607, 658
BshFI GGCC 1 cut(s) 731
BsiHKAI GWGCWC 2 cut(s) 486, 501
BslI CCNNNNNNNGG 3 cut(s) 34, 268, 391
BsmI GAATGC 4 cut(s) 598, 622, 649, 673
BsnI GGCC 1 cut(s) 731
Bsp1286I GDGCHC 3 cut(s) 241, 486, 501
Bsp143I GATC 4 cut(s) 93, 135, 372, 712
BspACI CCGC 1 cut(s) 131
BspANI GGCC 1 cut(s) 731
BspCNI CTCAG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 351
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 720
BsrDI GCAATG 2 cut(s) 330, 339
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 447, 587
BssMI GATC 4 cut(s) 93, 135, 372, 712
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 2 cut(s) 574, 689
Bst6I CTCTTC 3 cut(s) 108, 462, 716
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 283, 393
BstDEI CTNAG 1 cut(s) 41
BstKTI GATC 4 cut(s) 96, 138, 375, 715
BstMBI GATC 4 cut(s) 93, 135, 372, 712
BstMWI GCNNNNNNNGC 3 cut(s) 278, 320, 329
BstNI CCWGG 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 241
BstV1I GCAGC 4 cut(s) 154, 407, 607, 658
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BsuRI GGCC 1 cut(s) 731
BtgZI GCGATG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 283, 393
CsiI ACCWGGT 1 cut(s) 492
CviAII CATG 1 cut(s) 304
DdeI CTNAG 1 cut(s) 41
DpnI GATC 4 cut(s) 95, 137, 374, 714
DpnII GATC 4 cut(s) 93, 135, 372, 712
Eam1104I CTCTTC 3 cut(s) 108, 462, 716
EarI CTCTTC 3 cut(s) 108, 462, 716
EciI GGCGGA 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 3 cut(s) 89, 486, 522
EcoICRI GAGCTC 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 1 cut(s) 307
FaiI YATR 4 cut(s) 264, 279, 305, 410
FatI CATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 168, 396, 621, 672
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 4 cut(s) 168, 396, 621, 672
GluI GCNGC 4 cut(s) 168, 396, 621, 672
HaeIII GGCC 1 cut(s) 731
Hin1II CATG 1 cut(s) 307
HincII GTYRAC 3 cut(s) 191, 209, 685
HindII GTYRAC 3 cut(s) 191, 209, 685
HinfI GANTC 2 cut(s) 431, 695
HpaI GTTAAC 1 cut(s) 685
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 3 cut(s) 191, 209, 685
Hpy188I TCNGA 5 cut(s) 377, 430, 588, 694, 703
Hpy188III TCNNGA 5 cut(s) 13, 139, 385, 564, 716
Hpy8I GTNNAC 3 cut(s) 191, 209, 685
Hpy99I CGWCG 1 cut(s) 744
HpyCH4III ACNGT 2 cut(s) 574, 689
HpyCH4IV ACGT 4 cut(s) 88, 205, 705, 742
HpyCH4V TGCA 7 cut(s) 226, 281, 314, 356, 524, 560, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 320, 329
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 4 cut(s) 88, 205, 705, 742
Hsp92II CATG 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 685
Kzo9I GATC 4 cut(s) 93, 135, 372, 712
LmnI GCTCC 4 cut(s) 481, 547, 589, 664
LpnPI CCDG 9 cut(s) 28, 141, 254, 285, 366, 377, 398, 479, 506
Lsp1109I GCAGC 4 cut(s) 154, 407, 607, 658
LweI GCATC 3 cut(s) 107, 533, 569
MabI ACCWGGT 1 cut(s) 492
MaeII ACGT 4 cut(s) 88, 205, 705, 742
MaeIII GTNAC 1 cut(s) 158
MalI GATC 4 cut(s) 95, 137, 374, 714
MboI GATC 4 cut(s) 93, 135, 372, 712
MboII GAAGA 6 cut(s) 28, 31, 95, 479, 528, 733
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 3 cut(s) 241, 486, 501
MluCI AATT 3 cut(s) 122, 380, 555
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 366, 684
MslI CAYNNNNRTG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 494
Mva1269I GAATGC 4 cut(s) 598, 622, 649, 673
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 278, 320, 329
NdeII GATC 4 cut(s) 93, 135, 372, 712
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 1 cut(s) 351
PctI GAATGC 4 cut(s) 598, 622, 649, 673
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 695
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 4 cut(s) 169, 397, 622, 673
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 492
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 351
PsuI RGATCY 1 cut(s) 712
PsyI GACNNNGTC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 2 cut(s) 366, 684
SatI GCNGC 4 cut(s) 168, 396, 621, 672
Sau3AI GATC 4 cut(s) 93, 135, 372, 712
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 494
SduI GDGCHC 3 cut(s) 241, 486, 501
SexAI ACCWGGT 1 cut(s) 492
SfaNI GCATC 3 cut(s) 107, 533, 569
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 3 cut(s) 122, 380, 555
SsiI CCGC 1 cut(s) 131
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 492
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 574, 689
TaiI ACGT 4 cut(s) 91, 208, 708, 745
TaqI TCGA 2 cut(s) 501, 563
TasI AATT 3 cut(s) 122, 380, 555
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 2 cut(s) 366, 684
Tru9I TTAA 2 cut(s) 366, 684
TseI GCWGC 4 cut(s) 167, 395, 620, 671
TspDTI ATGAA 4 cut(s) 17, 320, 350, 456
Tth111I GACNNNGTC 1 cut(s) 204
XapI RAATTY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.