MD05G1267500.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018275
Physical Location & Seq
Forward (+)
40271110 .. 40272765
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1267500.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGTATTCAATCTGACAAACCCCAATTCAGATTTCATGGAAACTCCAGCCACTTCTGCAGTTTTACCACTTGGGTTCTTCCTTCTTCTCCACGTTTCAATGGCAGCTTCAGATCCAAATCCAACCCAAGACACCTTCCTCCAATGCCTTTCCAAATTTTCCCAAGATTCCTCCCCACCAATCTCTGCAGTTACGTATTTCCCCACCAACCCTTCATACACATCCATCTTATGTTCTTACATCAGAAACCTTCGCTTCACAAACCCCCAAACCCCAAAACCCTTATTCATAGTCGCCCCGACGAACTTTTCCCACATCCAAGCTTCAATCTTTTGCTCCAAAATCCATGCCCTGCAAGTGAGAATCCGCAGTGGGGGTCACGACTATGATGGCCTCTCTTATGTGTCTGAACTTCCGTTCATCATCATCGACATGTTTAGTCTTCGATCTATAAGTATCGACATAGAAGATGAGAGTGCTTGGGTTGAGTCTGGTGCGACACTCGGTGAAGTTTATTACAGAGTTGCAGAACAGAGCAAGATCCATGGCTTCCCAGCTGGTGTTTGCCCAACTGTTGGGGTTGGAGGGCACTTGAGTGGAGGCGGATATGGTAATATGATGAGAAAGTATGGCCTTTCTGTTGATAATATAATTGATGCTTTAATTGTTGATGTTAATGGAAGAATTTTAGATAGACAGTCAATGGGAGAAGACCTTTTTTGGGCTATTAGAGGTGGAGGCGGAGCTAGCTTTGGTGTGATTGTTTCTTGGAAAATCAAATTGGTTTCCGTCCCGGAAATTGTTACTGTTTTTAGAGTCGAGAGGACTTTAGAACAAGGCGCCACCGACATTGTTCATCGGTGGCAATATGTTGCTGATAGAATTCATGAAGACCTTTTCATTAGGGTGGTTGTGATGCCAGTGAATAAGAAGGTACATCAGACAGTGAAAGCCAAATTTGTGGCATTGTTTCTAGGAAATGCAGAAAGGCTTCATGCTTTGATGGATGAGAGCTTACCCCAATTGGGTTTGAAGGATGAGGATTATACCGAAATGAGCTGGATCGAATCGGTTTTGTATTGGTCTAATTACGCAATTGGAACTCCCGCTGATGTGTTGCTTGAAAGACAAGAAAAATCAAATAAGTTTCTGAAGAAGAAATCTGATTATGTCCAAGAGCCAATGTCGAAGGCAGGTCTTGAAGGACTTTGGAACAAAATGATGGAACTGAAAAAGCCAGTGCTAACATTCAATCCCTATGGAGGAAAAATGAGCGAAATTTCAGAGCTCGAAACCCCGTTTCCACATAGAGCAGGAAATGTGTACAAAATCCAGTACTCGGTGAATTGGAAAGAAGAGGGTGTGGAGGCTGCAGATAGAAATATTGATCTGATAAGAAGGCTTTATGAGTTTATGACTCCTTATGTCTCAAAGTCACCAAGGTGTTCATATCTGAATTATAGAGATGTTGACTTGGGTACAAATGGAAAGGGCAATGCAAGCTACTCAGAAGCTAGGGTTTGGGGGAGAAAATACTTCAATGGGAATTTTGACAGATTGGTACAAGTGAAGACTGCAGTAGATCCAGGAAACTTTTTTACTTATGAACAAAGTATTCCATCTCTTACAGCTTGGGAGGGTAAAATGGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

552

Amino Acids

61.94

Weight (kDa)

6.06

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 94 - 230 1e-21 FAD binding domain
BBE PF08031 483 - 540 8e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11010
malus_domestica MD05G1267500.v1.1
prunus_persica Prupe.4G098400_v2.0.a1
pyrus_communis pycom05g25050
rosa_chinensis RchiOBHm_Chr5g0017721
rosa_laevigata RLG00000032309
rosa_multiflora Rmu_sc0015477.1_g000001
rosa_roxburghii Rroxscaffold_1G00059770
rosa_rugosa Rorug05G0037500
rosa_wichuraiana Rw5G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1184
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 575
AciI CCGC 4 cut(s) 367, 603, 741, 1107
AclWI GGATC 4 cut(s) 107, 535, 1070, 1577
AcsI RAATTY 6 cut(s) 155, 684, 882, 956, 1278, 1546
AcuI CTGAAG 2 cut(s) 93, 1172
AcyI GRCGYC 1 cut(s) 840
AdeI CACNNNGTG 1 cut(s) 506
AfaI GTAC 5 cut(s) 936, 1325, 1337, 1480, 1563
AfiI CCNNNNNNNGG 6 cut(s) 373, 575, 721, 1025, 1262, 1339
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 8 cut(s) 10, 99, 327, 1033, 1124, 1202, 1252, 1540
AjnI CCWGG 1 cut(s) 1585
AjuI GAANNNNNNNTTGG 4 cut(s) 17, 49, 764, 796
AleI CACNNNNGTG 2 cut(s) 594, 1441
Alw21I GWGCWC 1 cut(s) 1290
Alw26I GTCTC 1 cut(s) 1432
AlwI GGATC 4 cut(s) 107, 535, 1070, 1577
AoxI GGCC 2 cut(s) 391, 631
ApeKI GCWGC 2 cut(s) 104, 1370
ApoI RAATTY 6 cut(s) 155, 684, 882, 956, 1278, 1546
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 842
AsuC2I CCSGG 1 cut(s) 794
AsuHPI GGTGA 3 cut(s) 518, 1354, 1428
AsuNHI GCTAGC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 591
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 1 cut(s) 1290
BbsI GAAGAC 4 cut(s) 434, 717, 897, 1577
Bbv12I GWGCWC 1 cut(s) 1290
BbvI GCAGC 2 cut(s) 116, 1357
BccI CCATC 5 cut(s) 233, 383, 997, 1216, 1627
BciT130I CCWGG 1 cut(s) 1587
BcnI CCSGG 1 cut(s) 794
BcoDI GTCTC 1 cut(s) 1432
BfaI CTAG 3 cut(s) 747, 974, 1515
BfmI CTRYAG 4 cut(s) 57, 186, 1371, 1575
BfoI RGCGCY 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 1184
BisI GCNGC 2 cut(s) 105, 1371
BlsI GCNGC 2 cut(s) 106, 1372
BmcAI AGTACT 1 cut(s) 1337
Bme1390I CCNGG 2 cut(s) 794, 1587
BmiI GGNNCC 1 cut(s) 841
BmrFI CCNGG 2 cut(s) 794, 1587
BmsI GCATC 2 cut(s) 646, 906
BmtI GCTAGC 1 cut(s) 750
BpiI GAAGAC 4 cut(s) 434, 717, 897, 1577
BpmI CTGGAG 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 794
BsaAI YACGTR 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 840
BsaJI CCNNGG 2 cut(s) 544, 1439
Bsc4I CCNNNNNNNGG 6 cut(s) 373, 575, 721, 1025, 1262, 1339
Bse1I ACTGG 3 cut(s) 920, 1238, 1333
Bse3DI GCAATG 1 cut(s) 1501
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 1587
BseDI CCNNGG 2 cut(s) 544, 1439
BseGI GGATG 4 cut(s) 221, 315, 1012, 1042
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 6 cut(s) 373, 575, 721, 1025, 1262, 1339
BseMI GCAATG 1 cut(s) 1501
BseMII CTCAG 2 cut(s) 1521, 1641
BseNI ACTGG 3 cut(s) 920, 1238, 1333
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 2 cut(s) 116, 1357
BseYI CCCAGC 1 cut(s) 553
BshFI GGCC 2 cut(s) 393, 633
BshNI GGYRCC 1 cut(s) 839
BsiHKAI GWGCWC 1 cut(s) 1290
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 6 cut(s) 373, 575, 721, 1025, 1262, 1339
BsmAI GTCTC 1 cut(s) 1432
BsmFI GGGAC 1 cut(s) 776
BsnI GGCC 2 cut(s) 393, 633
Bsp1286I GDGCHC 2 cut(s) 591, 1290
Bsp1407I TGTACA 1 cut(s) 1323
Bsp143I GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 4 cut(s) 367, 603, 741, 1107
BspANI GGCC 2 cut(s) 393, 633
BspCNI CTCAG 2 cut(s) 1520, 1642
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 1 cut(s) 841
BspMAI CTGCAG 4 cut(s) 61, 190, 1375, 1579
BspMI ACCTGC 1 cut(s) 1184
BspOI GCTAGC 1 cut(s) 750
BspPI GGATC 4 cut(s) 107, 535, 1070, 1577
BspT107I GGYRCC 1 cut(s) 839
BsrDI GCAATG 1 cut(s) 1501
BsrGI TGTACA 1 cut(s) 1323
BsrI ACTGG 3 cut(s) 920, 1238, 1333
BssECI CCNNGG 2 cut(s) 544, 1439
BssMI GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
BssNI GRCGYC 1 cut(s) 840
BssT1I CCWWGG 2 cut(s) 544, 1439
Bst2UI CCWGG 1 cut(s) 1587
Bst4CI ACNGT 4 cut(s) 574, 699, 808, 946
Bst6I CTCTTC 1 cut(s) 1350
BstACI GRCGYC 1 cut(s) 840
BstAUI TGTACA 1 cut(s) 1323
BstBAI YACGTR 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 748, 1501
BstDEI CTNAG 2 cut(s) 1507, 1650
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 4 cut(s) 221, 315, 1012, 1042
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 6 cut(s) 115, 449, 543, 1065, 1390, 1585
BstMAI GTCTC 1 cut(s) 1432
BstMBI GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
BstMWI GCNNNNNNNGC 3 cut(s) 56, 747, 1500
BstNI CCWGG 1 cut(s) 1587
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 792, 1585
BstSFI CTRYAG 4 cut(s) 57, 186, 1371, 1575
BstSLI GKGCMC 1 cut(s) 591
BstSNI TACGTA 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 116, 1357
BstV2I GAAGAC 4 cut(s) 434, 717, 897, 1577
BstX2I RGATCY 3 cut(s) 112, 540, 1582
BstXI CCANNNNNNTGG 1 cut(s) 961
BstYI RGATCY 3 cut(s) 112, 540, 1582
BsuRI GGCC 2 cut(s) 393, 633
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 4 cut(s) 221, 315, 1012, 1042
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 4 cut(s) 376, 927, 951, 1245
BveI ACCTGC 1 cut(s) 1184
Cac8I GCNNGC 2 cut(s) 748, 1501
CciI TCATGA 1 cut(s) 886
CfoI GCGC 1 cut(s) 842
Csp6I GTAC 5 cut(s) 935, 1324, 1336, 1479, 1562
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 6 cut(s) 37, 347, 433, 545, 887, 995
CviQI GTAC 5 cut(s) 935, 1324, 1336, 1479, 1562
DdeI CTNAG 2 cut(s) 1507, 1650
DinI GGCGCC 1 cut(s) 841
DpnI GATC 6 cut(s) 114, 448, 542, 1064, 1389, 1584
DpnII GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
DraIII CACNNNGTG 1 cut(s) 506
Eam1104I CTCTTC 1 cut(s) 1350
EarI CTCTTC 1 cut(s) 1350
EciI GGCGGA 2 cut(s) 618, 756
Ecl136II GAGCTC 1 cut(s) 1288
Eco105I TACGTA 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 544, 1439
Eco24I GRGCYC 1 cut(s) 1290
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 2 cut(s) 93, 1172
EcoICRI GAGCTC 1 cut(s) 1288
EcoRI GAATTC 1 cut(s) 882
EcoRII CCWGG 1 cut(s) 1585
EcoT14I CCWWGG 2 cut(s) 544, 1439
EcoT38I GRGCYC 1 cut(s) 1290
EgeI GGCGCC 1 cut(s) 841
EheI GGCGCC 1 cut(s) 841
ErhI CCWWGG 2 cut(s) 544, 1439
FaeI CATG 6 cut(s) 40, 350, 436, 548, 890, 998
FaqI GGGAC 1 cut(s) 776
FatI CATG 6 cut(s) 36, 346, 432, 544, 886, 994
FauI CCCGC 1 cut(s) 1114
Fnu4HI GCNGC 2 cut(s) 105, 1371
FokI GGATG 4 cut(s) 208, 302, 1019, 1049
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 2 cut(s) 105, 1371
FspBI CTAG 3 cut(s) 747, 974, 1515
GlaI GCGC 1 cut(s) 841
GluI GCNGC 2 cut(s) 105, 1371
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 2 cut(s) 393, 633
HapII CCGG 1 cut(s) 794
HhaI GCGC 1 cut(s) 842
Hin1I GRCGYC 1 cut(s) 840
Hin1II CATG 6 cut(s) 40, 350, 436, 548, 890, 998
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HincII GTYRAC 1 cut(s) 1471
HindII GTYRAC 1 cut(s) 1471
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 6 cut(s) 167, 363, 488, 816, 1067, 1417
HpaII CCGG 1 cut(s) 794
HphI GGTGA 3 cut(s) 518, 1354, 1428
Hpy166II GTNNAC 2 cut(s) 1324, 1471
Hpy188III TCNNGA 4 cut(s) 380, 820, 887, 1199
Hpy8I GTNNAC 2 cut(s) 1324, 1471
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 9 cut(s) 92, 145, 222, 260, 925, 1027, 1183, 1196, 1392
HpyCH4III ACNGT 4 cut(s) 574, 699, 808, 946
HpyCH4IV ACGT 2 cut(s) 93, 194
HpyCH4V TGCA 8 cut(s) 59, 188, 355, 527, 983, 1373, 1499, 1577
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 747, 1500
HpyF3I CTNAG 2 cut(s) 1507, 1650
HpySE526I ACGT 2 cut(s) 93, 194
Hsp92I GRCGYC 1 cut(s) 840
Hsp92II CATG 6 cut(s) 40, 350, 436, 548, 890, 998
HspAI GCGC 1 cut(s) 840
KasI GGCGCC 1 cut(s) 839
Kzo9I GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
LmnI GCTCC 2 cut(s) 341, 743
Lsp1109I GCAGC 2 cut(s) 116, 1357
LweI GCATC 2 cut(s) 646, 906
MaeI CTAG 3 cut(s) 747, 974, 1515
MaeII ACGT 2 cut(s) 93, 194
MaeIII GTNAC 4 cut(s) 190, 377, 802, 1434
MalI GATC 6 cut(s) 114, 448, 542, 1064, 1389, 1584
MboI GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
MfeI CAATTG 2 cut(s) 1022, 1095
MflI RGATCY 3 cut(s) 112, 540, 1582
MhlI GDGCHC 2 cut(s) 591, 1290
Mly113I GGCGCC 1 cut(s) 840
MlyI GAGTC 3 cut(s) 497, 825, 1411
MmeI TCCRAC 2 cut(s) 146, 562
MroXI GAANNNNTTC 1 cut(s) 990
MseI TTAA 2 cut(s) 662, 675
MslI CAYNNNNRTG 5 cut(s) 384, 431, 594, 905, 1441
MspA1I CMGCKG 2 cut(s) 557, 1109
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 2 cut(s) 794, 1587
MunI CAATTG 2 cut(s) 1022, 1095
MvaI CCWGG 1 cut(s) 1587
MwoI GCNNNNNNNGC 3 cut(s) 56, 747, 1500
NarI GGCGCC 1 cut(s) 840
NciI CCSGG 1 cut(s) 794
NcoI CCATGG 1 cut(s) 544
NdeII GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
NheI GCTAGC 1 cut(s) 746
NlaIII CATG 6 cut(s) 40, 350, 436, 548, 890, 998
NlaIV GGNNCC 1 cut(s) 841
NmuCI GTSAC 2 cut(s) 377, 1434
NspI RCATGY 1 cut(s) 436
OliI CACNNNNGTG 2 cut(s) 594, 1441
PagI TCATGA 1 cut(s) 886
PciI ACATGT 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 167, 363, 1067
PflMI CCANNNNNTGG 1 cut(s) 575
PfoI TCCNGGA 2 cut(s) 792, 1585
PkrI GCNGC 2 cut(s) 106, 1372
PleI GAGTC 3 cut(s) 496, 824, 1411
PluTI GGCGCC 1 cut(s) 843
PpsI GAGTC 3 cut(s) 496, 824, 1411
Ppu21I YACGTR 1 cut(s) 195
PscI ACATGT 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 1290
Psp6I CCWGG 1 cut(s) 1585
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 1 cut(s) 1585
PspN4I GGNNCC 1 cut(s) 841
PstI CTGCAG 4 cut(s) 61, 190, 1375, 1579
PsuI RGATCY 3 cut(s) 112, 540, 1582
PvuII CAGCTG 1 cut(s) 557
RsaI GTAC 5 cut(s) 936, 1325, 1337, 1480, 1563
RsaNI GTAC 5 cut(s) 935, 1324, 1336, 1479, 1562
RseI CAYNNNNRTG 5 cut(s) 384, 431, 594, 905, 1441
SacI GAGCTC 1 cut(s) 1290
SaqAI TTAA 2 cut(s) 662, 675
SatI GCNGC 2 cut(s) 105, 1371
Sau3AI GATC 6 cut(s) 112, 446, 540, 1062, 1387, 1582
ScaI AGTACT 1 cut(s) 1337
SchI GAGTC 3 cut(s) 497, 825, 1411
ScrFI CCNGG 2 cut(s) 794, 1587
SduI GDGCHC 2 cut(s) 591, 1290
SfaNI GCATC 2 cut(s) 646, 906
SfcI CTRYAG 4 cut(s) 57, 186, 1371, 1575
SfoI GGCGCC 1 cut(s) 841
SmiMI CAYNNNNRTG 5 cut(s) 384, 431, 594, 905, 1441
SmlI CTYRAG 1 cut(s) 592
SmoI CTYRAG 1 cut(s) 592
SnaBI TACGTA 1 cut(s) 195
SsiI CCGC 4 cut(s) 367, 603, 741, 1107
SspDI GGCGCC 1 cut(s) 839
SspI AATATT 1 cut(s) 1384
SspMI CTAG 3 cut(s) 747, 974, 1515
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 2 cut(s) 792, 1585
StyI CCWWGG 2 cut(s) 544, 1439
TaaI ACNGT 4 cut(s) 574, 699, 808, 946
TaiI ACGT 2 cut(s) 96, 197
TaqI TCGA 7 cut(s) 429, 445, 459, 819, 1065, 1187, 1290
TatI WGTACW 2 cut(s) 1323, 1335
TfiI GAWTC 3 cut(s) 167, 363, 1067
Tru1I TTAA 2 cut(s) 662, 675
Tru9I TTAA 2 cut(s) 662, 675
TscAI CASTG 4 cut(s) 376, 927, 951, 1245
TseFI GTSAC 2 cut(s) 377, 1434
TseI GCWGC 2 cut(s) 104, 1370
Tsp45I GTSAC 2 cut(s) 377, 1434
TspGWI ACGGA 2 cut(s) 405, 778
TspRI CASTG 4 cut(s) 376, 927, 951, 1245
Van91I CCANNNNNTGG 1 cut(s) 575
XapI RAATTY 6 cut(s) 155, 684, 882, 956, 1278, 1546
XceI RCATGY 1 cut(s) 436
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 3 cut(s) 747, 974, 1515
ZrmI AGTACT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.