pycom05g25050

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018275
Physical Location & Seq
Forward (+)
26525173 .. 26526792
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25050.1

Sequence Viewer

Length: 1620 bp
ATGGAAACTCGAGCCATTTCGGCAGTTTTACCCCTTGGGTTCTTCCTCCTTCTCCACGTTTCAATGGCAGCTTCAGATCCAAATCCAACCCAAGACACCTTCCTCCAATGCCTTTCCAATTTTTCCCAAGATTCCTCCCCACCAATCTCTGCAGTCACGTATTTCCCCACCAACACTTCATACACATCCATCTTAAATTCATACATCAGAAACCTTCGCTTCACAGACCCCCAAACCTCAAAACCCTTATTCATAGTCGCCCCGACAAACTTTTCCCACATCCAAGCTTCAATCTTTTGCTCCAAAATCCATGCCCTGCAAGTGAGAATCCGCAGTGGGGGTCACGACTATGATGGCCTCTCTTATGTGTCTGAACTTCCGTTCATCATCATCGACATGTTTAGTCTTCGATCTATAAGTATCGACATAGAAGATGAGAGTGCTTGGGTTGAGTCTGGTGCGACACTCGGTGAAGTTTATTACAGAGTTGCAGAACAGAGCAAGATCCATGGCTTCCCAGCTGGTGTTTGCCCGACTGTTGGGGTTGGAGGGCACTTGAGCGGAGGCGGATATGGTAATATGATGAGAAAGTATGGCCTTTCTGTTGATAATATAATTGATGCTTTAATTGTTGATGTTAATGGAAGAATCTTAGATAGACAGTCAATGGGAGAAGACCTTTTTTGGGCTATTAGAGGTGGAGGCGGAGCTAGCTTTGGTGTGATTGTTTCTTGGAAAATCAAATTGGTTTCCGTCCCGGAAATTGTCACTGTTTTTAGAGTCGAGAGGACTTTAGAACAAGGCGCCACCGACATTGTTCATCGGTGGCAATATGTTGCTGATAGAATTCATGAAGACCTTTTCATTAGGGTGGTTGTGATGCCAGGGAATAAGAAGGGACATCAGACAGTGAAAGCCAAATTTGTGGCATTGTTTCTAGGAAATGCAGAAAGGCTTCATGCTTTGATGGATGAGAGCTTACCCCAATTGGGTTTGAAGGATGAGGATTATTCCGAAATGAGCTGGATCGAATCGGTTTTGTATTGGTCTAATTACGCAATTGGAACTCCCACTGATGTGTTGCTTGAAAGACAAGAAAAATCAAGAAAGTTTCTGAAGAAGAAATCTGATTATGTCCAAGAGCCAATGTCAAAGGCAGGTCTTGAAGGACTCTGGAACAAAATGATGGAACTGAAAAAGCCAGTGCTAACATTCAATCCCTATGGAGGAAAAATGAGCGAAATTTCAGAGCTCGAAACCCCGTTTCCACATAGAGCAGGAAATGTGTACAAAATCCAGTACTCGGTGAATTGGAAAGAAGAGGGTGTGGAGGCTGCAGACAGAAATATTGATCTGATAAGAAGGCTTTATGAGTTTATGACTCCTTATGTCTCAAAGTCACCAAGGTGTTCATATCTGAATTATAGAGATGTTGACTTGGGTACAAATGGAAATGGCAATGCAAGCTACTCAGAAGCTAGGGTTTGGGGGAGAAAATACTTCAATGGGAATTTTGACAGATTGGTACAAGTGAAGACTGCAGTAGATCCAGGAAACTTTTTTAGTTATGAACAAAGTATTCCATCTCTTACAGCTTGGGAGGGTAAAATGGCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

540

Amino Acids

60.57

Weight (kDa)

6.06

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 82 - 218 9.7e-22 FAD binding domain
BBE PF08031 471 - 528 9.8e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11010
malus_domestica MD05G1267500.v1.1
prunus_persica Prupe.4G098400_v2.0.a1
pyrus_communis pycom05g25050
rosa_chinensis RchiOBHm_Chr5g0017721
rosa_laevigata RLG00000032309
rosa_multiflora Rmu_sc0015477.1_g000001
rosa_roxburghii Rroxscaffold_1G00059770
rosa_rugosa Rorug05G0037500
rosa_wichuraiana Rw5G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1148
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 1 cut(s) 561
AciI CCGC 4 cut(s) 331, 561, 567, 705
AclWI GGATC 4 cut(s) 71, 499, 1034, 1541
AcoI YGGCCR 1 cut(s) 1611
AcsI RAATTY 5 cut(s) 196, 846, 920, 1242, 1510
AcuI CTGAAG 2 cut(s) 57, 1136
AcyI GRCGYC 1 cut(s) 804
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 4 cut(s) 1289, 1301, 1444, 1527
AfiI CCNNNNNNNGG 6 cut(s) 337, 539, 685, 989, 1226, 1303
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 7 cut(s) 63, 291, 997, 1088, 1166, 1216, 1504
AjnI CCWGG 2 cut(s) 883, 1549
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AleI CACNNNNGTG 2 cut(s) 1076, 1405
Alw21I GWGCWC 1 cut(s) 1254
Alw26I GTCTC 1 cut(s) 1396
AlwI GGATC 4 cut(s) 71, 499, 1034, 1541
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 3 cut(s) 355, 595, 1611
ApeKI GCWGC 2 cut(s) 68, 1334
ApoI RAATTY 5 cut(s) 196, 846, 920, 1242, 1510
Asp700I GAANNNNTTC 1 cut(s) 954
AspLEI GCGC 1 cut(s) 806
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 3 cut(s) 482, 1318, 1392
AsuNHI GCTAGC 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 555
BanI GGYRCC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 1254
BbsI GAAGAC 4 cut(s) 398, 681, 861, 1541
Bbv12I GWGCWC 1 cut(s) 1254
BbvI GCAGC 2 cut(s) 80, 1321
BccI CCATC 5 cut(s) 197, 347, 961, 1180, 1591
BciT130I CCWGG 2 cut(s) 885, 1551
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 1 cut(s) 1396
BfaI CTAG 3 cut(s) 711, 938, 1479
BfmI CTRYAG 3 cut(s) 150, 1335, 1539
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 1148
BglI GCCNNNNNGGC 1 cut(s) 20
BisI GCNGC 2 cut(s) 69, 1335
BlsI GCNGC 2 cut(s) 70, 1336
BmcAI AGTACT 1 cut(s) 1301
Bme1390I CCNGG 3 cut(s) 758, 885, 1551
BmeT110I CYCGRG 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 805
BmrFI CCNGG 3 cut(s) 758, 885, 1551
BmsI GCATC 2 cut(s) 610, 870
BmtI GCTAGC 1 cut(s) 714
BpiI GAAGAC 4 cut(s) 398, 681, 861, 1541
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 758
BsaAI YACGTR 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 804
BsaJI CCNNGG 4 cut(s) 34, 508, 884, 1403
Bsc4I CCNNNNNNNGG 6 cut(s) 337, 539, 685, 989, 1226, 1303
Bse1I ACTGG 2 cut(s) 1202, 1297
Bse3DI GCAATG 1 cut(s) 1465
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 2 cut(s) 885, 1551
BseDI CCNNGG 4 cut(s) 34, 508, 884, 1403
BseGI GGATG 4 cut(s) 185, 279, 976, 1006
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 6 cut(s) 337, 539, 685, 989, 1226, 1303
BseMI GCAATG 1 cut(s) 1465
BseMII CTCAG 1 cut(s) 1485
BseNI ACTGG 2 cut(s) 1202, 1297
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 2 cut(s) 80, 1321
BseYI CCCAGC 1 cut(s) 517
BshFI GGCC 3 cut(s) 357, 597, 1613
BshNI GGYRCC 1 cut(s) 803
BsiHKAI GWGCWC 1 cut(s) 1254
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 2 cut(s) 740, 912
BslI CCNNNNNNNGG 6 cut(s) 337, 539, 685, 989, 1226, 1303
BsmAI GTCTC 1 cut(s) 1396
BsmFI GGGAC 2 cut(s) 740, 912
BsnI GGCC 3 cut(s) 357, 597, 1613
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 555, 1254
Bsp1407I TGTACA 1 cut(s) 1287
Bsp143I GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 4 cut(s) 331, 561, 567, 705
BspANI GGCC 3 cut(s) 357, 597, 1613
BspCNI CTCAG 1 cut(s) 1484
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 1 cut(s) 805
BspMAI CTGCAG 3 cut(s) 154, 1339, 1543
BspMI ACCTGC 1 cut(s) 1148
BspOI GCTAGC 1 cut(s) 714
BspPI GGATC 4 cut(s) 71, 499, 1034, 1541
BspT107I GGYRCC 1 cut(s) 803
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 1 cut(s) 1465
BsrGI TGTACA 1 cut(s) 1287
BsrI ACTGG 2 cut(s) 1202, 1297
BssECI CCNNGG 4 cut(s) 34, 508, 884, 1403
BssMI GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
BssNI GRCGYC 1 cut(s) 804
BssT1I CCWWGG 3 cut(s) 34, 508, 1403
Bst2UI CCWGG 2 cut(s) 885, 1551
Bst4CI ACNGT 4 cut(s) 538, 663, 772, 910
Bst6I CTCTTC 1 cut(s) 1314
BstACI GRCGYC 1 cut(s) 804
BstAUI TGTACA 1 cut(s) 1287
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 712, 1465
BstDEI CTNAG 2 cut(s) 652, 1471
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 4 cut(s) 185, 279, 976, 1006
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 6 cut(s) 79, 413, 507, 1029, 1354, 1549
BstMAI GTCTC 1 cut(s) 1396
BstMBI GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
BstMWI GCNNNNNNNGC 3 cut(s) 20, 711, 1464
BstNI CCWGG 2 cut(s) 885, 1551
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 756, 883, 1549
BstSFI CTRYAG 3 cut(s) 150, 1335, 1539
BstSLI GKGCMC 1 cut(s) 555
BstV1I GCAGC 2 cut(s) 80, 1321
BstV2I GAAGAC 4 cut(s) 398, 681, 861, 1541
BstX2I RGATCY 3 cut(s) 76, 504, 1546
BstXI CCANNNNNNTGG 1 cut(s) 925
BstYI RGATCY 3 cut(s) 76, 504, 1546
BsuRI GGCC 3 cut(s) 357, 597, 1613
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 4 cut(s) 185, 279, 976, 1006
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 5 cut(s) 340, 768, 915, 1071, 1209
BveI ACCTGC 1 cut(s) 1148
Cac8I GCNNGC 2 cut(s) 712, 1465
CciI TCATGA 1 cut(s) 850
CfoI GCGC 1 cut(s) 806
Csp6I GTAC 4 cut(s) 1288, 1300, 1443, 1526
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 5 cut(s) 311, 397, 509, 851, 959
CviQI GTAC 4 cut(s) 1288, 1300, 1443, 1526
DdeI CTNAG 2 cut(s) 652, 1471
DinI GGCGCC 1 cut(s) 805
DpnI GATC 6 cut(s) 78, 412, 506, 1028, 1353, 1548
DpnII GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
DraIII CACNNNGTG 1 cut(s) 470
EaeI YGGCCR 1 cut(s) 1611
Eam1104I CTCTTC 1 cut(s) 1314
EarI CTCTTC 1 cut(s) 1314
EciI GGCGGA 2 cut(s) 582, 720
Ecl136II GAGCTC 1 cut(s) 1252
Eco130I CCWWGG 3 cut(s) 34, 508, 1403
Eco24I GRGCYC 1 cut(s) 1254
Eco53kI GAGCTC 1 cut(s) 1252
Eco57I CTGAAG 2 cut(s) 57, 1136
Eco88I CYCGRG 1 cut(s) 9
EcoICRI GAGCTC 1 cut(s) 1252
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 2 cut(s) 883, 1549
EcoT14I CCWWGG 3 cut(s) 34, 508, 1403
EcoT38I GRGCYC 1 cut(s) 1254
EgeI GGCGCC 1 cut(s) 805
EheI GGCGCC 1 cut(s) 805
ErhI CCWWGG 3 cut(s) 34, 508, 1403
FaeI CATG 5 cut(s) 314, 400, 512, 854, 962
FaqI GGGAC 2 cut(s) 740, 912
FatI CATG 5 cut(s) 310, 396, 508, 850, 958
Fnu4HI GCNGC 2 cut(s) 69, 1335
FokI GGATG 4 cut(s) 172, 266, 983, 1013
FriOI GRGCYC 1 cut(s) 1254
Fsp4HI GCNGC 2 cut(s) 69, 1335
FspBI CTAG 3 cut(s) 711, 938, 1479
GlaI GCGC 1 cut(s) 805
GluI GCNGC 2 cut(s) 69, 1335
GsaI CCCAGC 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 3 cut(s) 357, 597, 1613
HapII CCGG 1 cut(s) 758
HhaI GCGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 5 cut(s) 314, 400, 512, 854, 962
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 1435
HindII GTYRAC 1 cut(s) 1435
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 8 cut(s) 131, 327, 452, 648, 780, 1031, 1170, 1381
HpaII CCGG 1 cut(s) 758
HphI GGTGA 3 cut(s) 482, 1318, 1392
Hpy166II GTNNAC 2 cut(s) 1288, 1435
Hpy188III TCNNGA 6 cut(s) 344, 784, 851, 1104, 1163, 1174
Hpy8I GTNNAC 2 cut(s) 1288, 1435
HpyAV CCTTC 7 cut(s) 59, 109, 224, 889, 991, 1160, 1356
HpyCH4III ACNGT 4 cut(s) 538, 663, 772, 910
HpyCH4IV ACGT 2 cut(s) 57, 158
HpyCH4V TGCA 7 cut(s) 152, 319, 491, 947, 1337, 1463, 1541
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 711, 1464
HpyF3I CTNAG 2 cut(s) 652, 1471
HpySE526I ACGT 2 cut(s) 57, 158
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 5 cut(s) 314, 400, 512, 854, 962
HspAI GCGC 1 cut(s) 804
KasI GGCGCC 1 cut(s) 803
Kzo9I GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
LmnI GCTCC 2 cut(s) 305, 707
Lsp1109I GCAGC 2 cut(s) 80, 1321
LweI GCATC 2 cut(s) 610, 870
MaeI CTAG 3 cut(s) 711, 938, 1479
MaeII ACGT 2 cut(s) 57, 158
MaeIII GTNAC 4 cut(s) 154, 341, 766, 1398
MalI GATC 6 cut(s) 78, 412, 506, 1028, 1353, 1548
MbiI CCGCTC 1 cut(s) 561
MboI GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
MfeI CAATTG 2 cut(s) 986, 1059
MflI RGATCY 3 cut(s) 76, 504, 1546
MhlI GDGCHC 2 cut(s) 555, 1254
Mly113I GGCGCC 1 cut(s) 804
MlyI GAGTC 4 cut(s) 461, 789, 1164, 1375
MmeI TCCRAC 2 cut(s) 110, 526
MroXI GAANNNNTTC 1 cut(s) 954
MseI TTAA 3 cut(s) 194, 626, 639
MslI CAYNNNNRTG 5 cut(s) 348, 395, 869, 1076, 1405
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 3 cut(s) 758, 885, 1551
MunI CAATTG 2 cut(s) 986, 1059
MvaI CCWGG 2 cut(s) 885, 1551
MwoI GCNNNNNNNGC 3 cut(s) 20, 711, 1464
NarI GGCGCC 1 cut(s) 804
NciI CCSGG 1 cut(s) 758
NcoI CCATGG 1 cut(s) 508
NdeII GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
NheI GCTAGC 1 cut(s) 710
NlaIII CATG 5 cut(s) 314, 400, 512, 854, 962
NlaIV GGNNCC 1 cut(s) 805
NmuCI GTSAC 4 cut(s) 154, 341, 766, 1398
NspI RCATGY 1 cut(s) 400
OliI CACNNNNGTG 2 cut(s) 1076, 1405
PaeR7I CTCGAG 1 cut(s) 9
PagI TCATGA 1 cut(s) 850
PciI ACATGT 1 cut(s) 396
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 4 cut(s) 131, 327, 648, 1031
PfoI TCCNGGA 2 cut(s) 756, 1549
PkrI GCNGC 2 cut(s) 70, 1336
PleI GAGTC 4 cut(s) 460, 788, 1164, 1375
PluTI GGCGCC 1 cut(s) 807
PpsI GAGTC 4 cut(s) 460, 788, 1164, 1375
Ppu21I YACGTR 1 cut(s) 159
PscI ACATGT 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 1254
Psp6I CCWGG 2 cut(s) 883, 1549
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 2 cut(s) 883, 1549
PspN4I GGNNCC 1 cut(s) 805
PspXI VCTCGAGB 1 cut(s) 9
PstI CTGCAG 3 cut(s) 154, 1339, 1543
PsuI RGATCY 3 cut(s) 76, 504, 1546
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 1289, 1301, 1444, 1527
RsaNI GTAC 4 cut(s) 1288, 1300, 1443, 1526
RseI CAYNNNNRTG 5 cut(s) 348, 395, 869, 1076, 1405
SacI GAGCTC 1 cut(s) 1254
SaqAI TTAA 3 cut(s) 194, 626, 639
SatI GCNGC 2 cut(s) 69, 1335
Sau3AI GATC 6 cut(s) 76, 410, 504, 1026, 1351, 1546
ScaI AGTACT 1 cut(s) 1301
SchI GAGTC 4 cut(s) 461, 789, 1164, 1375
ScrFI CCNGG 3 cut(s) 758, 885, 1551
SduI GDGCHC 2 cut(s) 555, 1254
SfaNI GCATC 2 cut(s) 610, 870
SfcI CTRYAG 3 cut(s) 150, 1335, 1539
SfoI GGCGCC 1 cut(s) 805
Sfr274I CTCGAG 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 9
SmiMI CAYNNNNRTG 5 cut(s) 348, 395, 869, 1076, 1405
SmlI CTYRAG 2 cut(s) 9, 556
SmoI CTYRAG 2 cut(s) 9, 556
SsiI CCGC 4 cut(s) 331, 561, 567, 705
SspDI GGCGCC 1 cut(s) 803
SspI AATATT 1 cut(s) 1348
SspMI CTAG 3 cut(s) 711, 938, 1479
SstI GAGCTC 1 cut(s) 1254
StyD4I CCNGG 3 cut(s) 756, 883, 1549
StyI CCWWGG 3 cut(s) 34, 508, 1403
TaaI ACNGT 4 cut(s) 538, 663, 772, 910
TaiI ACGT 2 cut(s) 60, 161
TaqI TCGA 7 cut(s) 10, 393, 409, 423, 783, 1029, 1254
TatI WGTACW 2 cut(s) 1287, 1299
TfiI GAWTC 4 cut(s) 131, 327, 648, 1031
Tru1I TTAA 3 cut(s) 194, 626, 639
Tru9I TTAA 3 cut(s) 194, 626, 639
TscAI CASTG 5 cut(s) 340, 775, 915, 1078, 1209
TseFI GTSAC 4 cut(s) 154, 341, 766, 1398
TseI GCWGC 2 cut(s) 68, 1334
Tsp45I GTSAC 4 cut(s) 154, 341, 766, 1398
TspGWI ACGGA 2 cut(s) 369, 742
TspRI CASTG 5 cut(s) 340, 775, 915, 1078, 1209
XapI RAATTY 5 cut(s) 196, 846, 920, 1242, 1510
XceI RCATGY 1 cut(s) 400
XhoI CTCGAG 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 954
XspI CTAG 3 cut(s) 711, 938, 1479
ZrmI AGTACT 1 cut(s) 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.