Rroxscaffold_1G00059770

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018275
Physical Location & Seq
Forward (+)
81852800 .. 81854409
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059770.1

Sequence Viewer

Length: 1503 bp
ATGAAAACCCAAACTGCTTCTGCAACTTTGGCAATTGTCTCCTTCCTTCTTCTCCATGCTTCCATGGCAGCTTCAGATCCAATCCAAGACACCTTCCTCCAATGCCTCTCCAATCTTTCCCAATCCCCGTCTCCACCAATCTCATCAGTCACCTATTTCCCCACCAACCCTTCATACACACCAATCTTACATTCCTACATAAGAAACCTTAGATTCACAACCCCTAAAACCCCAAAACCCTCATTCATTGTCACTCCAACCCATGTCTCTCACATCCAAGCCTCAATCCTCTGCTCCAAAATCCATAACCTTCAAGTCAGAATCCGCAGCGGTGGCCATGACTATGATGGCCTCTCTTACATCTCAGATGTTCCATTCATCATTATTGACATGTTCAATCTTCGATCCATAACAGTCAACATAGAAGAAGAAAGTGCTTGGGTCGAGTCTGGAGCAACACTAGGTGAAGTTTATTACAGAATAGCTGAGAAGAGCAAAATACATGGCTACCCAGCTGGGGTTTGTCCCACAGTGGGAGTCGGAGGACACTTGAGTGGAGGTGGGTATGGAAATATGATGAGGAAACATGGCTTATCAGTTGATAACATTCTTGATGCTCAAATAGTTGATGTTAATGGAAGGGTTTTGGATAGAGAGTCAATGGGGGAAGACCTCTTTGGGCTATTAGAGGTGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCTTGGAAAATCAAATTGGTTCAAGTCCCAGAAGTTGTCACAGTTTTTAGAGTTGAGAGGACTTTGGAGCAAGGCGCCACCGACATTGTTCATCAGTGGCAGAAATGCAGAAATGCTTGAAGCTTTGATGGGTGAGAGGTTTCCCCAATTGGGTTTGAAGCATGAGGACTATGTCGAACTGGGTTGGATTGAATCGGTTCTGTATTGGTCTAACTACCCAATTGGAACTTCTGCAGATATACTGCTTGAAAGAATACCTCAGTCAGAAAAGTTCTTGAAGAAGAAATCTGACTACATCCAAGAACCAATGTCAAAGGCTGGTCTTGAAGGACTGTGGAACAAGATGATGGAGCTGAAAAAGCCAGTACTAACATTCAATCCTTATGGTGGCAAAATGAATGAAATTTCGGAGCTTGACACCCCATTCCCACATAGAGCAGGAAATGTATACAAAATTCAGTACTCGGTGAATTGGAAAGAGGAAGGTGTAGAGGCTGAAGATAAACATCTTGATCTGATAAGAAGGCTTTACGAGCATATGACACCTTATGTGTCAAAGTCACCAAGGTGTTCATATCTAAATTACAGAGATGTTGATTTGGGAACTAATGGAAATGGCAAAGTGAGCTACTCAGAAGCTAGTGTTTGGGGGACCAAGTACTTCAAGGGAAACTTTGAGAGGTTGGTACAAGTGAAGGCTGCTGTTGATCCAGGAAACTTTTTCAGGTATGAACAAAGTATCCCACCTCTTACGGCTTGGAATGGTAAAATGGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

500

Amino Acids

56.17

Weight (kDa)

6.38

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 80 - 216 3.6e-23 FAD binding domain
BBE PF08031 432 - 489 1.9e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11010
malus_domestica MD05G1267500.v1.1
prunus_persica Prupe.4G098400_v2.0.a1
pyrus_communis pycom05g25050
rosa_chinensis RchiOBHm_Chr5g0017721
rosa_laevigata RLG00000032309
rosa_multiflora Rmu_sc0015477.1_g000001
rosa_roxburghii Rroxscaffold_1G00059770
rosa_rugosa Rorug05G0037500
rosa_wichuraiana Rw5G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 796
AccI GTMKAC 1 cut(s) 1170
AciI CCGC 2 cut(s) 325, 330
AclWI GGATC 3 cut(s) 71, 399, 1424
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 2 cut(s) 1125, 1176
AcuI CTGAAG 2 cut(s) 57, 1239
AcyI GRCGYC 1 cut(s) 797
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 4 cut(s) 1089, 1184, 1382, 1410
AfiI CCNNNNNNNGG 4 cut(s) 517, 533, 872, 1109
AflIII ACRYGT 1 cut(s) 390
AjnI CCWGG 1 cut(s) 1432
AjuI GAANNNNNNNTTGG 8 cut(s) 175, 207, 660, 692, 721, 753, 934, 966
AleI CACNNNNGTG 2 cut(s) 552, 1288
AloI GAACNNNNNNTCC 2 cut(s) 1446, 1478
AluBI AGCT 9 cut(s) 71, 485, 515, 703, 845, 1075, 1135, 1350, 1361
AluI AGCT 9 cut(s) 71, 485, 515, 703, 845, 1075, 1135, 1350, 1361
Alw26I GTCTC 3 cut(s) 43, 135, 271
AlwI GGATC 3 cut(s) 71, 399, 1424
AoxI GGCC 2 cut(s) 334, 349
ApeKI GCWGC 3 cut(s) 68, 327, 1421
ApoI RAATTY 2 cut(s) 1125, 1176
Asp700I GAANNNNTTC 1 cut(s) 918
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 1 cut(s) 1374
AsuHPI GGTGA 5 cut(s) 142, 476, 866, 1201, 1275
AvaII GGWCC 1 cut(s) 1374
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 1 cut(s) 796
BbsI GAAGAC 1 cut(s) 675
BbvI GCAGC 3 cut(s) 80, 339, 1408
BccI CCATC 3 cut(s) 341, 844, 1063
BceAI ACGGC 1 cut(s) 1491
BciT130I CCWGG 1 cut(s) 1434
BciVI GTATCC 1 cut(s) 1472
BcoDI GTCTC 3 cut(s) 43, 135, 271
BfaI CTAG 3 cut(s) 461, 704, 1362
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 800
BfuI GTATCC 1 cut(s) 1472
BisI GCNGC 3 cut(s) 69, 328, 1422
BlsI GCNGC 3 cut(s) 70, 329, 1423
BmcAI AGTACT 3 cut(s) 1089, 1184, 1382
Bme1390I CCNGG 1 cut(s) 1434
Bme18I GGWCC 1 cut(s) 1374
BmgT120I GGNCC 1 cut(s) 1374
BmiI GGNNCC 2 cut(s) 798, 1375
BmrFI CCNGG 1 cut(s) 1434
BmrI ACTGGG 1 cut(s) 911
BmsI GCATC 1 cut(s) 604
BmuI ACTGGG 1 cut(s) 911
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 1227
BsaHI GRCGYC 1 cut(s) 797
BsaJI CCNNGG 2 cut(s) 63, 1286
Bsc4I CCNNNNNNNGG 4 cut(s) 517, 533, 872, 1109
Bse1I ACTGG 2 cut(s) 906, 1085
Bse8I GATNNNNATC 1 cut(s) 1227
BseBI CCWGG 1 cut(s) 1434
BseDI CCNNGG 2 cut(s) 63, 1286
BseGI GGATG 2 cut(s) 273, 1017
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 4 cut(s) 517, 533, 872, 1109
BseMII CTCAG 4 cut(s) 378, 477, 995, 1368
BseNI ACTGG 2 cut(s) 906, 1085
BseXI GCAGC 3 cut(s) 80, 339, 1408
BseYI CCCAGC 2 cut(s) 511, 515
BshFI GGCC 2 cut(s) 336, 351
BshNI GGYRCC 1 cut(s) 796
BslFI GGGAC 3 cut(s) 510, 733, 1387
BslI CCNNNNNNNGG 4 cut(s) 517, 533, 872, 1109
BsmAI GTCTC 3 cut(s) 43, 135, 271
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 3 cut(s) 510, 733, 1387
BsnI GGCC 2 cut(s) 336, 351
Bsp143I GATC 4 cut(s) 76, 404, 1234, 1429
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 2 cut(s) 325, 330
BspANI GGCC 2 cut(s) 336, 351
BspCNI CTCAG 4 cut(s) 377, 478, 994, 1367
BspLI GGNNCC 2 cut(s) 798, 1375
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 3 cut(s) 71, 399, 1424
BspQI GCTCTTC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 796
BsrI ACTGG 2 cut(s) 906, 1085
BssECI CCNNGG 2 cut(s) 63, 1286
BssMI GATC 4 cut(s) 76, 404, 1234, 1429
BssNAI GTATAC 1 cut(s) 1171
BssNI GRCGYC 1 cut(s) 797
BssT1I CCWWGG 2 cut(s) 63, 1286
Bst1107I GTATAC 1 cut(s) 1171
Bst2UI CCWGG 1 cut(s) 1434
Bst4CI ACNGT 4 cut(s) 415, 532, 765, 1056
Bst6I CTCTTC 1 cut(s) 485
BstACI GRCGYC 1 cut(s) 797
BstDEI CTNAG 5 cut(s) 209, 364, 486, 981, 1354
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 273, 1017
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 4 cut(s) 79, 407, 1237, 1432
BstMAI GTCTC 3 cut(s) 43, 135, 271
BstMBI GATC 4 cut(s) 76, 404, 1234, 1429
BstMWI GCNNNNNNNGC 6 cut(s) 29, 65, 333, 1081, 1255, 1347
BstNI CCWGG 1 cut(s) 1434
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 1432
BstSFI CTRYAG 1 cut(s) 954
BstV1I GCAGC 3 cut(s) 80, 339, 1408
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BstZ17I GTATAC 1 cut(s) 1171
BsuI GTATCC 1 cut(s) 1472
BsuRI GGCC 2 cut(s) 336, 351
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 273, 1017
BtsIMutI CAGTG 2 cut(s) 537, 823
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 1 cut(s) 1374
Csp6I GTAC 4 cut(s) 1088, 1183, 1381, 1409
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 8 cut(s) 56, 64, 263, 338, 391, 503, 587, 884
CviQI GTAC 4 cut(s) 1088, 1183, 1381, 1409
DdeI CTNAG 5 cut(s) 209, 364, 486, 981, 1354
DinI GGCGCC 1 cut(s) 798
DpnI GATC 4 cut(s) 78, 406, 1236, 1431
DpnII GATC 4 cut(s) 76, 404, 1234, 1429
DraIII CACNNNGTG 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 63, 1286
Eco47I GGWCC 1 cut(s) 1374
Eco57I CTGAAG 2 cut(s) 57, 1239
EcoRII CCWGG 1 cut(s) 1432
EcoT14I CCWWGG 2 cut(s) 63, 1286
EgeI GGCGCC 1 cut(s) 798
EheI GGCGCC 1 cut(s) 798
ErhI CCWWGG 2 cut(s) 63, 1286
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 8 cut(s) 59, 67, 266, 341, 394, 506, 590, 887
FalI AAGNNNNNCTT 2 cut(s) 1379, 1411
FaqI GGGAC 3 cut(s) 510, 733, 1387
FatI CATG 8 cut(s) 55, 63, 262, 337, 390, 502, 586, 883
FauNDI CATATG 1 cut(s) 1260
FblI GTMKAC 1 cut(s) 1170
Fnu4HI GCNGC 3 cut(s) 69, 328, 1422
FokI GGATG 2 cut(s) 260, 1004
Fsp4HI GCNGC 3 cut(s) 69, 328, 1422
FspBI CTAG 3 cut(s) 461, 704, 1362
GlaI GCGC 1 cut(s) 798
GluI GCNGC 3 cut(s) 69, 328, 1422
GsaI CCCAGC 2 cut(s) 515, 519
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 800
HaeIII GGCC 2 cut(s) 336, 351
HhaI GCGC 1 cut(s) 799
Hin1I GRCGYC 1 cut(s) 797
Hin1II CATG 8 cut(s) 59, 67, 266, 341, 394, 506, 590, 887
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 6 cut(s) 213, 321, 446, 537, 656, 914
HphI GGTGA 5 cut(s) 142, 476, 866, 1201, 1275
Hpy166II GTNNAC 2 cut(s) 418, 1171
Hpy188I TCNGA 9 cut(s) 76, 320, 367, 542, 988, 1012, 1132, 1239, 1357
Hpy188III TCNNGA 5 cut(s) 450, 611, 997, 1046, 1232
Hpy8I GTNNAC 2 cut(s) 418, 1171
HpyCH4III ACNGT 4 cut(s) 415, 532, 765, 1056
HpyCH4V TGCA 3 cut(s) 23, 830, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 65, 333, 1081, 1255, 1347
HpyF3I CTNAG 5 cut(s) 209, 364, 486, 981, 1354
Hsp92I GRCGYC 1 cut(s) 797
Hsp92II CATG 8 cut(s) 59, 67, 266, 341, 394, 506, 590, 887
HspAI GCGC 1 cut(s) 797
KasI GGCGCC 1 cut(s) 796
Kzo9I GATC 4 cut(s) 76, 404, 1234, 1429
LguI GCTCTTC 1 cut(s) 485
LmnI GCTCC 6 cut(s) 299, 452, 700, 789, 1072, 1132
Lsp1109I GCAGC 3 cut(s) 80, 339, 1408
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 461, 704, 1362
MaeIII GTNAC 4 cut(s) 148, 250, 759, 1281
MalI GATC 4 cut(s) 78, 406, 1236, 1431
MboI GATC 4 cut(s) 76, 404, 1234, 1429
MboII GAAGA 9 cut(s) 41, 392, 437, 440, 502, 680, 1012, 1015, 1232
MfeI CAATTG 3 cut(s) 33, 869, 942
MflI RGATCY 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 8 cut(s) 33, 736, 869, 942, 1125, 1176, 1192, 1303
MluNI TGGCCA 1 cut(s) 336
Mly113I GGCGCC 1 cut(s) 797
MlyI GAGTC 3 cut(s) 455, 546, 665
MmeI TCCRAC 3 cut(s) 281, 520, 887
Mox20I TGGCCA 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 918
MscI TGGCCA 1 cut(s) 336
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 3 cut(s) 342, 552, 1288
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 330, 515
MspR9I CCNGG 1 cut(s) 1434
MunI CAATTG 3 cut(s) 33, 869, 942
MvaI CCWGG 1 cut(s) 1434
MwoI GCNNNNNNNGC 6 cut(s) 29, 65, 333, 1081, 1255, 1347
NarI GGCGCC 1 cut(s) 797
NcoI CCATGG 1 cut(s) 63
NdeI CATATG 1 cut(s) 1260
NdeII GATC 4 cut(s) 76, 404, 1234, 1429
NlaIII CATG 8 cut(s) 59, 67, 266, 341, 394, 506, 590, 887
NlaIV GGNNCC 2 cut(s) 798, 1375
NmuCI GTSAC 4 cut(s) 148, 250, 759, 1281
NspI RCATGY 1 cut(s) 394
OliI CACNNNNGTG 2 cut(s) 552, 1288
PciI ACATGT 1 cut(s) 390
PciSI GCTCTTC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 3 cut(s) 213, 321, 914
PflFI GACNNNGTC 1 cut(s) 893
PfoI TCCNGGA 1 cut(s) 1432
PkrI GCNGC 3 cut(s) 70, 329, 1423
PleI GAGTC 3 cut(s) 454, 545, 664
PluTI GGCGCC 1 cut(s) 800
PpsI GAGTC 3 cut(s) 454, 545, 664
PscI ACATGT 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 1432
PspFI CCCAGC 2 cut(s) 511, 515
PspGI CCWGG 1 cut(s) 1432
PspN4I GGNNCC 2 cut(s) 798, 1375
PspPI GGNCC 1 cut(s) 1374
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 4 cut(s) 1089, 1184, 1382, 1410
RsaNI GTAC 4 cut(s) 1088, 1183, 1381, 1409
RseI CAYNNNNRTG 3 cut(s) 342, 552, 1288
SapI GCTCTTC 1 cut(s) 485
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 3 cut(s) 69, 328, 1422
Sau3AI GATC 4 cut(s) 76, 404, 1234, 1429
Sau96I GGNCC 1 cut(s) 1374
ScaI AGTACT 3 cut(s) 1089, 1184, 1382
SchI GAGTC 3 cut(s) 455, 546, 665
ScrFI CCNGG 1 cut(s) 1434
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 1 cut(s) 954
SfoI GGCGCC 1 cut(s) 798
SinI GGWCC 1 cut(s) 1374
SmiMI CAYNNNNRTG 3 cut(s) 342, 552, 1288
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 8 cut(s) 33, 736, 869, 942, 1125, 1176, 1192, 1303
SsiI CCGC 2 cut(s) 325, 330
SspDI GGCGCC 1 cut(s) 796
SspMI CTAG 3 cut(s) 461, 704, 1362
StyD4I CCNGG 1 cut(s) 1432
StyI CCWWGG 2 cut(s) 63, 1286
TaaI ACNGT 4 cut(s) 415, 532, 765, 1056
TaqI TCGA 3 cut(s) 403, 444, 897
TasI AATT 8 cut(s) 33, 736, 869, 942, 1125, 1176, 1192, 1303
TatI WGTACW 3 cut(s) 1087, 1182, 1380
TfiI GAWTC 3 cut(s) 213, 321, 914
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 2 cut(s) 537, 823
TseFI GTSAC 4 cut(s) 148, 250, 759, 1281
TseI GCWGC 3 cut(s) 68, 327, 1421
Tsp45I GTSAC 4 cut(s) 148, 250, 759, 1281
TspDTI ATGAA 9 cut(s) 17, 162, 235, 367, 802, 1133, 1137, 1284, 1467
TspRI CASTG 2 cut(s) 537, 823
Tth111I GACNNNGTC 1 cut(s) 893
VpaK11BI GGWCC 1 cut(s) 1374
XapI RAATTY 2 cut(s) 1125, 1176
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 3 cut(s) 461, 704, 1362
ZrmI AGTACT 3 cut(s) 1089, 1184, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.