RLG00000032309

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018275
Physical Location & Seq
Reverse (-)
13386605 .. 13388155
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032309

Sequence Viewer

Length: 1551 bp
ATGGCAGCTTCAGATCCAAACCAAGACACCTTCCTCCAATGCCTCTCCAATCTTTCCCAATCCCCCTCTCCACCAATCTCATCAGTCACCTATTTCCCCACCAACCCTTCATACACACCAATCTTACATTCCTACATAAGAAACCTTAGATTCACAACCCCTAAAACCCCAAAACCCTCATTCATTGTCACTCCAACCCATGTTTCTCACATCCAAGCCTCAATCCTCTGCTCCAAAATCCATAACCTTCAAGTCAGAATCCGCAGCGGTGGCCATGACTATGATGGCCTCTCTTACATCTCAGATGTTCCATTCATCATTATTGACATGTTCAATCTTCGATCCATAACAGTCAACATAGAAGATGAAAGTGCTTGGGTCGAGTCTGGAGCAACACTAGGTGAAGTTTATTACAGTATAGCTGAGCAGAGCAAAATACATGGCTACCCAGCTGGGGTTTGTCCCACAGTGGGAGTCGGAGGACACTTGAGTGGAGGTGGGTATGGAAATATGATGAGGAAACATGGCTTATCAGTTGATAACATTCTTGATGCTCATATAGTTGATGTTAATGGAAGGGTTTTGGATAGAAAATCAATGGGGGAAGACCTCTTTTGGGCTATTAGAGGTGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCTTGGAAAATCAAATTGGTTCAAGTCCCAGAAGTTGTCACAGTTTTTAGAGTTGAGAGGACTTTGGAGCAAGGCGCCACCGACATTGTTCATCAATGGCAGTATGTAGGGGATACAATAGATGATGATCTTTTCATTAGGGTGGTTGTGATGCCAGTCAATAGAAAAACCCAGAAGACAGTGAAAGCTAAATTTGTTGCTTTGTACTTAGGAAATGCAGAAAAGCTTGAAGCTTTGATGGGTGAGAGGTTTCCCCAATTGGGTTTGAAGCATGAGGACTATGTCGAAATGGGTTGGATTGAATCGGTTCTGTATTGGTCTAACTACCCAATTGGAACTTCTGCAGATATACTGCTTGAAAGAATACCTCAGTCAGAAAAGTTCTTGAAGAAGAAATCTGACTACATCCAAGAACCAATGTCAAAGGCTGGTCTTGAAGGACTGTGGAACAAGATGATGGAGCTGAAAAAACCAGTACTAACATTCAATCCTTATGGTGGGAAAATGAGCGAAATTTCAGAGCTTGACACCCCATTCCCACATAGAGCAGGAAATGTGTACAAAATTCAGTACTCGGTGAATTGGAAAGAGGAAGGTGTAGAGGCTGAAGATAAACATCTTGATCTGATAAGAAGGCTTTACGAGCATATGACACCTTATGTGTCAAAGTCACCAAGGTGTTCATATCTAAATTACAGAGATGTTGATTTGGGGACTAATGGAAATGGCAATGTGAGCTACTCAGAAGCTAGTGTTTGGGGGACCAAGTATTTCAAGGGAAACTTTGAGAGGTTGGTACAAGTGAAGACTGCTGTTGATCCAGGAAACTTTTTCAGGTATGAACAAAGTATCCCACCTCTTACGGCTTGGAATGGTAAAATGGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

517

Amino Acids

58.0

Weight (kDa)

6.41

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 59 - 195 8.2e-23 FAD binding domain
BBE PF08031 448 - 505 1.7e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11010
malus_domestica MD05G1267500.v1.1
prunus_persica Prupe.4G098400_v2.0.a1
pyrus_communis pycom05g25050
rosa_chinensis RchiOBHm_Chr5g0017721
rosa_laevigata RLG00000032309
rosa_multiflora Rmu_sc0015477.1_g000001
rosa_roxburghii Rroxscaffold_1G00059770
rosa_rugosa Rorug05G0037500
rosa_wichuraiana Rw5G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AciI CCGC 2 cut(s) 262, 267
AclWI GGATC 3 cut(s) 8, 336, 1472
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 851, 1173, 1224
AcuI CTGAAG 1 cut(s) 1287
AcyI GRCGYC 1 cut(s) 735
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 5 cut(s) 866, 1137, 1220, 1232, 1458
AfiI CCNNNNNNNGG 5 cut(s) 454, 470, 616, 920, 1157
AflIII ACRYGT 1 cut(s) 327
AjnI CCWGG 1 cut(s) 1480
AjuI GAANNNNNNNTTGG 8 cut(s) 112, 144, 187, 219, 659, 691, 982, 1014
AleI CACNNNNGTG 2 cut(s) 489, 1336
AloI GAACNNNNNNTCC 2 cut(s) 1494, 1526
AlwI GGATC 3 cut(s) 8, 336, 1472
AoxI GGCC 2 cut(s) 271, 286
ApeKI GCWGC 2 cut(s) 5, 264
ApoI RAATTY 3 cut(s) 851, 1173, 1224
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 1422
AsuHPI GGTGA 5 cut(s) 79, 413, 914, 1249, 1323
AvaII GGWCC 1 cut(s) 1422
BalI TGGCCA 1 cut(s) 273
BanI GGYRCC 1 cut(s) 734
BbsI GAAGAC 3 cut(s) 612, 842, 1472
BbvI GCAGC 2 cut(s) 17, 276
BccI CCATC 3 cut(s) 278, 892, 1111
BceAI ACGGC 1 cut(s) 1539
BciT130I CCWGG 1 cut(s) 1482
BciVI GTATCC 2 cut(s) 766, 1520
BfaI CTAG 3 cut(s) 398, 642, 1410
BfmI CTRYAG 1 cut(s) 1002
BfoI RGCGCY 1 cut(s) 738
BfuI GTATCC 2 cut(s) 766, 1520
BisI GCNGC 2 cut(s) 6, 265
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 2 cut(s) 7, 266
BmcAI AGTACT 2 cut(s) 1137, 1232
Bme1390I CCNGG 1 cut(s) 1482
Bme18I GGWCC 1 cut(s) 1422
BmgT120I GGNCC 1 cut(s) 1422
BmiI GGNNCC 2 cut(s) 736, 1423
BmrFI CCNGG 1 cut(s) 1482
BmsI GCATC 2 cut(s) 541, 801
BpiI GAAGAC 3 cut(s) 612, 842, 1472
BpmI CTGGAG 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 508
BsaBI GATNNNNATC 2 cut(s) 786, 1275
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 1 cut(s) 1334
Bsc4I CCNNNNNNNGG 5 cut(s) 454, 470, 616, 920, 1157
Bse1I ACTGG 2 cut(s) 815, 1133
Bse3DI GCAATG 1 cut(s) 1396
Bse8I GATNNNNATC 2 cut(s) 786, 1275
BseBI CCWGG 1 cut(s) 1482
BseDI CCNNGG 1 cut(s) 1334
BseGI GGATG 2 cut(s) 210, 1065
BseJI GATNNNNATC 2 cut(s) 786, 1275
BseLI CCNNNNNNNGG 5 cut(s) 454, 470, 616, 920, 1157
BseMI GCAATG 1 cut(s) 1396
BseMII CTCAG 4 cut(s) 315, 414, 1043, 1416
BseNI ACTGG 2 cut(s) 815, 1133
BseXI GCAGC 2 cut(s) 17, 276
BseYI CCCAGC 2 cut(s) 448, 452
BshFI GGCC 2 cut(s) 273, 288
BshNI GGYRCC 1 cut(s) 734
BslFI GGGAC 4 cut(s) 447, 671, 1387, 1435
BslI CCNNNNNNNGG 5 cut(s) 454, 470, 616, 920, 1157
BsmFI GGGAC 4 cut(s) 447, 671, 1387, 1435
BsnI GGCC 2 cut(s) 273, 288
Bsp1407I TGTACA 1 cut(s) 1218
Bsp143I GATC 5 cut(s) 13, 341, 787, 1282, 1477
Bsp1720I GCTNAGC 1 cut(s) 423
BspACI CCGC 2 cut(s) 262, 267
BspANI GGCC 2 cut(s) 273, 288
BspCNI CTCAG 4 cut(s) 314, 415, 1042, 1415
BspLI GGNNCC 2 cut(s) 736, 1423
BspMAI CTGCAG 1 cut(s) 1006
BspPI GGATC 3 cut(s) 8, 336, 1472
BspT107I GGYRCC 1 cut(s) 734
BsrDI GCAATG 1 cut(s) 1396
BsrGI TGTACA 1 cut(s) 1218
BsrI ACTGG 2 cut(s) 815, 1133
BssECI CCNNGG 1 cut(s) 1334
BssMI GATC 5 cut(s) 13, 341, 787, 1282, 1477
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 1334
Bst2UI CCWGG 1 cut(s) 1482
Bst4CI ACNGT 6 cut(s) 352, 416, 469, 703, 841, 1104
BstACI GRCGYC 1 cut(s) 735
BstAUI TGTACA 1 cut(s) 1218
BstDEI CTNAG 6 cut(s) 146, 301, 423, 868, 1029, 1402
BstF5I GGATG 2 cut(s) 210, 1065
BstH2I RGCGCY 1 cut(s) 738
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 5 cut(s) 16, 344, 790, 1285, 1480
BstMBI GATC 5 cut(s) 13, 341, 787, 1282, 1477
BstMWI GCNNNNNNNGC 3 cut(s) 270, 1303, 1395
BstNI CCWGG 1 cut(s) 1482
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 1480
BstSFI CTRYAG 1 cut(s) 1002
BstV1I GCAGC 2 cut(s) 17, 276
BstV2I GAAGAC 3 cut(s) 612, 842, 1472
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuI GTATCC 2 cut(s) 766, 1520
BsuRI GGCC 2 cut(s) 273, 288
BtsCI GGATG 2 cut(s) 210, 1065
BtsIMutI CAGTG 2 cut(s) 474, 846
CfoI GCGC 1 cut(s) 737
Cfr13I GGNCC 1 cut(s) 1422
Csp6I GTAC 5 cut(s) 865, 1136, 1219, 1231, 1457
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 6 cut(s) 200, 275, 328, 440, 524, 932
CviQI GTAC 5 cut(s) 865, 1136, 1219, 1231, 1457
DdeI CTNAG 6 cut(s) 146, 301, 423, 868, 1029, 1402
DinI GGCGCC 1 cut(s) 736
DpnI GATC 5 cut(s) 15, 343, 789, 1284, 1479
DpnII GATC 5 cut(s) 13, 341, 787, 1282, 1477
DraIII CACNNNGTG 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 1334
Eco47I GGWCC 1 cut(s) 1422
Eco57I CTGAAG 1 cut(s) 1287
EcoRII CCWGG 1 cut(s) 1480
EcoT14I CCWWGG 1 cut(s) 1334
EgeI GGCGCC 1 cut(s) 736
EheI GGCGCC 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 1334
FaeI CATG 6 cut(s) 203, 278, 331, 443, 527, 935
FalI AAGNNNNNCTT 2 cut(s) 1427, 1459
FaqI GGGAC 4 cut(s) 447, 671, 1387, 1435
FatI CATG 6 cut(s) 199, 274, 327, 439, 523, 931
FauNDI CATATG 1 cut(s) 1308
Fnu4HI GCNGC 2 cut(s) 6, 265
FokI GGATG 2 cut(s) 197, 1052
Fsp4HI GCNGC 2 cut(s) 6, 265
FspBI CTAG 3 cut(s) 398, 642, 1410
GlaI GCGC 1 cut(s) 736
GluI GCNGC 2 cut(s) 6, 265
GsaI CCCAGC 2 cut(s) 452, 456
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 738
HaeIII GGCC 2 cut(s) 273, 288
HhaI GCGC 1 cut(s) 737
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 6 cut(s) 203, 278, 331, 443, 527, 935
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 2 cut(s) 884, 891
HinfI GANTC 5 cut(s) 150, 258, 383, 474, 962
HphI GGTGA 5 cut(s) 79, 413, 914, 1249, 1323
Hpy166II GTNNAC 2 cut(s) 355, 1219
Hpy188I TCNGA 9 cut(s) 13, 257, 304, 479, 1036, 1060, 1180, 1287, 1405
Hpy188III TCNNGA 5 cut(s) 387, 548, 1045, 1094, 1280
Hpy8I GTNNAC 2 cut(s) 355, 1219
HpyAV CCTTC 7 cut(s) 40, 117, 257, 570, 1091, 1247, 1287
HpyCH4III ACNGT 6 cut(s) 352, 416, 469, 703, 841, 1104
HpyCH4V TGCA 2 cut(s) 878, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 270, 1303, 1395
HpyF3I CTNAG 6 cut(s) 146, 301, 423, 868, 1029, 1402
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 6 cut(s) 203, 278, 331, 443, 527, 935
HspAI GCGC 1 cut(s) 735
KasI GGCGCC 1 cut(s) 734
Kzo9I GATC 5 cut(s) 13, 341, 787, 1282, 1477
LmnI GCTCC 5 cut(s) 236, 389, 638, 727, 1120
Lsp1109I GCAGC 2 cut(s) 17, 276
LweI GCATC 2 cut(s) 541, 801
MaeI CTAG 3 cut(s) 398, 642, 1410
MaeIII GTNAC 4 cut(s) 85, 187, 697, 1329
MalI GATC 5 cut(s) 15, 343, 789, 1284, 1479
MboI GATC 5 cut(s) 13, 341, 787, 1282, 1477
MboII GAAGA 8 cut(s) 329, 374, 617, 847, 1060, 1063, 1280, 1477
MfeI CAATTG 2 cut(s) 917, 990
MflI RGATCY 1 cut(s) 13
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 8 cut(s) 674, 851, 917, 990, 1173, 1224, 1240, 1351
MluNI TGGCCA 1 cut(s) 273
Mly113I GGCGCC 1 cut(s) 735
MlyI GAGTC 2 cut(s) 392, 483
MmeI TCCRAC 3 cut(s) 218, 457, 935
Mox20I TGGCCA 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 966
MscI TGGCCA 1 cut(s) 273
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 4 cut(s) 279, 489, 800, 1336
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 267, 452
MspR9I CCNGG 1 cut(s) 1482
MunI CAATTG 2 cut(s) 917, 990
MvaI CCWGG 1 cut(s) 1482
MwoI GCNNNNNNNGC 3 cut(s) 270, 1303, 1395
NarI GGCGCC 1 cut(s) 735
NdeI CATATG 1 cut(s) 1308
NdeII GATC 5 cut(s) 13, 341, 787, 1282, 1477
NlaIII CATG 6 cut(s) 203, 278, 331, 443, 527, 935
NlaIV GGNNCC 2 cut(s) 736, 1423
NmuCI GTSAC 4 cut(s) 85, 187, 697, 1329
NspI RCATGY 1 cut(s) 331
OliI CACNNNNGTG 2 cut(s) 489, 1336
PciI ACATGT 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 3 cut(s) 150, 258, 962
PflFI GACNNNGTC 1 cut(s) 941
PfoI TCCNGGA 1 cut(s) 1480
PkrI GCNGC 2 cut(s) 7, 266
PleI GAGTC 2 cut(s) 391, 482
PluTI GGCGCC 1 cut(s) 738
PpsI GAGTC 2 cut(s) 391, 482
PscI ACATGT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 1480
PspFI CCCAGC 2 cut(s) 448, 452
PspGI CCWGG 1 cut(s) 1480
PspN4I GGNNCC 2 cut(s) 736, 1423
PspPI GGNCC 1 cut(s) 1422
PstI CTGCAG 1 cut(s) 1006
PsuI RGATCY 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 941
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 5 cut(s) 866, 1137, 1220, 1232, 1458
RsaNI GTAC 5 cut(s) 865, 1136, 1219, 1231, 1457
RseI CAYNNNNRTG 4 cut(s) 279, 489, 800, 1336
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 2 cut(s) 6, 265
Sau3AI GATC 5 cut(s) 13, 341, 787, 1282, 1477
Sau96I GGNCC 1 cut(s) 1422
ScaI AGTACT 2 cut(s) 1137, 1232
SchI GAGTC 2 cut(s) 392, 483
ScrFI CCNGG 1 cut(s) 1482
SfaNI GCATC 2 cut(s) 541, 801
SfcI CTRYAG 1 cut(s) 1002
SfoI GGCGCC 1 cut(s) 736
SinI GGWCC 1 cut(s) 1422
SmiMI CAYNNNNRTG 4 cut(s) 279, 489, 800, 1336
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
Sse9I AATT 8 cut(s) 674, 851, 917, 990, 1173, 1224, 1240, 1351
SsiI CCGC 2 cut(s) 262, 267
SspDI GGCGCC 1 cut(s) 734
SspMI CTAG 3 cut(s) 398, 642, 1410
StyD4I CCNGG 1 cut(s) 1480
StyI CCWWGG 1 cut(s) 1334
TaaI ACNGT 6 cut(s) 352, 416, 469, 703, 841, 1104
TaqI TCGA 3 cut(s) 340, 381, 945
TasI AATT 8 cut(s) 674, 851, 917, 990, 1173, 1224, 1240, 1351
TatI WGTACW 4 cut(s) 864, 1135, 1218, 1230
TfiI GAWTC 3 cut(s) 150, 258, 962
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 2 cut(s) 474, 846
TseFI GTSAC 4 cut(s) 85, 187, 697, 1329
TseI GCWGC 2 cut(s) 5, 264
Tsp45I GTSAC 4 cut(s) 85, 187, 697, 1329
TspDTI ATGAA 8 cut(s) 99, 172, 304, 381, 740, 784, 1332, 1515
TspRI CASTG 2 cut(s) 474, 846
Tth111I GACNNNGTC 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 1422
XapI RAATTY 3 cut(s) 851, 1173, 1224
XceI RCATGY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 3 cut(s) 398, 642, 1410
ZrmI AGTACT 2 cut(s) 1137, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.