RchiOBHm_Chr5g0017721

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018275
Physical Location & Seq
Reverse (-)
12311207 .. 12313144
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29797

Sequence Viewer

Length: 1653 bp
ATGATGATCAATGTGGCAAAACCCAGCTCCCAAATAGTCATGAAAACCCAAACTACTTCTGCAACTTTGGCAATTGTCTCCTCCCTTCTTCTCCATGCTTCCATGGCAGCTTCAGATCCAATCCAAGACACCTTCCTCCAATGCCTCTCCAATCTTTCCCAATCCCCCTCTCCACCAATCTCATCAGTCACCTATTTCCCCACCAACCCTTCATACACACCAATCTTACATTCCTACATTAGAAACCTTAGATTCACAACCCCTAAAACCCCAAAACCCTCATTCATTGTCACTCCAACCCATGTCTCTCACATCCAAGCCTCAATCCTCTGCTCCAAAATCCACAACCTTCAAGTCAGAATCCGCAGCGGTGGCCATGACTATGATGGCCTCTCTTACATCTCAGATGTTCCATTCATCATTATTGACATGTTCAATCTTCGATCCATAACAGTCAACGTAGAAGATGAAAGTGCTTGGGTCGAGTCTGGAGCAACACTAGGTGAAGTTTATTACAGAATAGCTGAGCAGAGTAAAATCCATGGCTATCCAGCTGGGGTTTGTCCCACAGTGGGAGTTGGAGGACATTTAAGTGGAGGTGGGTATGGAAATATGATGAGGAAACATGGCTTATCAGTTGATAACATTCTTGATGCTCAAATAGTTGATGTTAATGGAAGTGTTTTGGATAGAGAGTCAATGGGGGAAGACCTCTTTTGGGCTATTAGAGGTGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCTTGGAAAATCAAATTGGTTCAAGTCCCAGAAGTTGTCACAGTTTTTAGAGTTGAGAGGACTTTGGAGCAAGGCGCCACCGACATTGTTCATCAATGGCAGTACGTAGGGGATACAATAGATGATGATCTTTTCATTAGGGTGGTTGTGATGCCAGTCAATAGAAAAACCCAGAAGACAGTGAAAGCTAAATTTGTAGCTTTGTATTTAGGAAATGCAGAAAAGCTTGAAGCTTTGATGGGTGAGAGGTTTCCCCAATTGGGTTTGAAGCATGAGGACTATGTCGAAAGGGGTTGGATTGAATCGGTTCTGTATTGGTCTAACTACCCAATTGGAACTTCTGCAGATATACTGCTTGAAAGAATACCTCAGTCAGAAAAGTTCTTGAAGAAGAAATCTGACTACATCCAAGAACCAATGTCAAAGGCTGGTCTTGAAGGACTGTGGAACAAGATGATGGAGCTGAAAAAGCCAGTACTAACATTCAATCCTTATGGTGGGAAAATGAGCGAGATTCCGGAGCTTGACACCCCATTCCCACATAGAGCAGGAAATGTGTACAAAATTCAGTACTCGGTGAATTGGAAAGAGGAAGGTGTAGAGGCTGAAGATAAACATCTTGATCTGATAAGAAGGCTTTATGAGCATATGACACCTTATGTGTCAACGTCACCAAGGTGTTCATATCTAAATTACAGAGATGTTGATTTGGGGACTAATGGAAATGGCAATGTGAGCTACTCAGAAGCTAGTGTTTGGGGGACCAAGTACTTCAAGGGAAACTTTGAGAGGTTGGTACAAGTGAAGACTGCTGTTGATCCAGGAAACTTTTTCAGGTATGAACAAAGTATCCCACCTCTTACGGCTTGGAATGGTAAAATGGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

550

Amino Acids

61.54

Weight (kDa)

6.42

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 93 - 229 3.9e-23 FAD binding domain
BBE PF08031 482 - 539 1.9e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018275)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11010
malus_domestica MD05G1267500.v1.1
prunus_persica Prupe.4G098400_v2.0.a1
pyrus_communis pycom05g25050
rosa_chinensis RchiOBHm_Chr5g0017721
rosa_laevigata RLG00000032309
rosa_multiflora Rmu_sc0015477.1_g000001
rosa_roxburghii Rroxscaffold_1G00059770
rosa_rugosa Rorug05G0037500
rosa_wichuraiana Rw5G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 836
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 2 cut(s) 364, 369
AclWI GGATC 3 cut(s) 110, 438, 1574
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 2 cut(s) 953, 1326
AcuI CTGAAG 2 cut(s) 96, 1389
AcyI GRCGYC 1 cut(s) 837
AdeI CACNNNGTG 1 cut(s) 503
AfaI GTAC 6 cut(s) 866, 1239, 1322, 1334, 1532, 1560
AfiI CCNNNNNNNGG 4 cut(s) 572, 718, 1022, 1259
AflIII ACRYGT 1 cut(s) 429
AjnI CCWGG 1 cut(s) 1582
AjuI GAANNNNNNNTTGG 6 cut(s) 214, 246, 761, 793, 1084, 1116
AleI CACNNNNGTG 1 cut(s) 1438
AloI GAACNNNNNNTCC 2 cut(s) 1596, 1628
Alw26I GTCTC 2 cut(s) 82, 310
AlwI GGATC 3 cut(s) 110, 438, 1574
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 2 cut(s) 373, 388
ApeKI GCWGC 2 cut(s) 107, 366
ApoI RAATTY 2 cut(s) 953, 1326
Asp700I GAANNNNTTC 1 cut(s) 1068
AspLEI GCGC 1 cut(s) 839
AspS9I GGNCC 1 cut(s) 1524
AsuHPI GGTGA 5 cut(s) 181, 515, 1016, 1351, 1425
AvaII GGWCC 1 cut(s) 1524
BalI TGGCCA 1 cut(s) 375
BanI GGYRCC 1 cut(s) 836
BbsI GAAGAC 3 cut(s) 714, 944, 1574
BbvI GCAGC 2 cut(s) 119, 378
BccI CCATC 3 cut(s) 380, 994, 1213
BceAI ACGGC 1 cut(s) 1641
BciT130I CCWGG 1 cut(s) 1584
BciVI GTATCC 2 cut(s) 868, 1622
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 82, 310
BfaI CTAG 3 cut(s) 500, 744, 1512
BfmI CTRYAG 1 cut(s) 1104
BfoI RGCGCY 1 cut(s) 840
BfuI GTATCC 2 cut(s) 868, 1622
BisI GCNGC 2 cut(s) 108, 367
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 2 cut(s) 109, 368
BmcAI AGTACT 3 cut(s) 1239, 1334, 1532
Bme1390I CCNGG 1 cut(s) 1584
Bme18I GGWCC 1 cut(s) 1524
BmgT120I GGNCC 1 cut(s) 1524
BmiI GGNNCC 2 cut(s) 838, 1525
BmrFI CCNGG 1 cut(s) 1584
BmsI GCATC 2 cut(s) 643, 903
BpiI GAAGAC 3 cut(s) 714, 944, 1574
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 868
BsaBI GATNNNNATC 2 cut(s) 888, 1377
BsaHI GRCGYC 1 cut(s) 837
BsaJI CCNNGG 3 cut(s) 102, 541, 1436
BsaWI WCCGGW 1 cut(s) 1279
Bsc4I CCNNNNNNNGG 4 cut(s) 572, 718, 1022, 1259
Bse1I ACTGG 2 cut(s) 917, 1235
Bse3DI GCAATG 1 cut(s) 1498
Bse8I GATNNNNATC 2 cut(s) 888, 1377
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 1 cut(s) 1584
BseDI CCNNGG 3 cut(s) 102, 541, 1436
BseGI GGATG 2 cut(s) 312, 1167
BseJI GATNNNNATC 2 cut(s) 888, 1377
BseLI CCNNNNNNNGG 4 cut(s) 572, 718, 1022, 1259
BseMI GCAATG 1 cut(s) 1498
BseMII CTCAG 4 cut(s) 417, 516, 1145, 1518
BseNI ACTGG 2 cut(s) 917, 1235
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 119, 378
BseYI CCCAGC 2 cut(s) 23, 554
BshFI GGCC 2 cut(s) 375, 390
BshNI GGYRCC 1 cut(s) 836
BsiSI CCGG 1 cut(s) 1280
BslFI GGGAC 4 cut(s) 549, 773, 1489, 1537
BslI CCNNNNNNNGG 4 cut(s) 572, 718, 1022, 1259
BsmAI GTCTC 2 cut(s) 82, 310
BsmFI GGGAC 4 cut(s) 549, 773, 1489, 1537
BsnI GGCC 2 cut(s) 375, 390
Bsp13I TCCGGA 1 cut(s) 1279
Bsp1407I TGTACA 1 cut(s) 1320
Bsp143I GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 2 cut(s) 102, 541
BspACI CCGC 2 cut(s) 364, 369
BspANI GGCC 2 cut(s) 375, 390
BspCNI CTCAG 4 cut(s) 416, 517, 1144, 1517
BspEI TCCGGA 1 cut(s) 1279
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 838, 1525
BspMAI CTGCAG 1 cut(s) 1108
BspPI GGATC 3 cut(s) 110, 438, 1574
BspT107I GGYRCC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 1498
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 2 cut(s) 917, 1235
BssECI CCNNGG 3 cut(s) 102, 541, 1436
BssMI GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
BssNI GRCGYC 1 cut(s) 837
BssT1I CCWWGG 3 cut(s) 102, 541, 1436
Bst2UI CCWGG 1 cut(s) 1584
Bst4CI ACNGT 5 cut(s) 454, 571, 805, 943, 1206
BstACI GRCGYC 1 cut(s) 837
BstAUI TGTACA 1 cut(s) 1320
BstBAI YACGTR 1 cut(s) 868
BstDEI CTNAG 5 cut(s) 248, 403, 525, 1131, 1504
BstDSI CCRYGG 2 cut(s) 102, 541
BstF5I GGATG 2 cut(s) 312, 1167
BstH2I RGCGCY 1 cut(s) 840
BstHHI GCGC 1 cut(s) 839
BstKTI GATC 6 cut(s) 9, 118, 446, 892, 1387, 1582
BstMAI GTCTC 2 cut(s) 82, 310
BstMBI GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
BstMWI GCNNNNNNNGC 6 cut(s) 68, 104, 372, 1231, 1405, 1497
BstNI CCWGG 1 cut(s) 1584
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 1582
BstSFI CTRYAG 1 cut(s) 1104
BstSNI TACGTA 1 cut(s) 868
BstV1I GCAGC 2 cut(s) 119, 378
BstV2I GAAGAC 3 cut(s) 714, 944, 1574
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 2 cut(s) 868, 1622
BsuRI GGCC 2 cut(s) 375, 390
BtgI CCRYGG 2 cut(s) 102, 541
BtsCI GGATG 2 cut(s) 312, 1167
BtsIMutI CAGTG 2 cut(s) 576, 948
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 839
Cfr13I GGNCC 1 cut(s) 1524
Csp6I GTAC 6 cut(s) 865, 1238, 1321, 1333, 1531, 1559
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviAII CATG 9 cut(s) 40, 95, 103, 302, 377, 430, 542, 626, 1034
CviQI GTAC 6 cut(s) 865, 1238, 1321, 1333, 1531, 1559
DdeI CTNAG 5 cut(s) 248, 403, 525, 1131, 1504
DinI GGCGCC 1 cut(s) 838
DpnI GATC 6 cut(s) 8, 117, 445, 891, 1386, 1581
DpnII GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
DraIII CACNNNGTG 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 373
Eco105I TACGTA 1 cut(s) 868
Eco130I CCWWGG 3 cut(s) 102, 541, 1436
Eco47I GGWCC 1 cut(s) 1524
Eco57I CTGAAG 2 cut(s) 96, 1389
EcoRII CCWGG 1 cut(s) 1582
EcoT14I CCWWGG 3 cut(s) 102, 541, 1436
EgeI GGCGCC 1 cut(s) 838
EheI GGCGCC 1 cut(s) 838
ErhI CCWWGG 3 cut(s) 102, 541, 1436
FaeI CATG 9 cut(s) 43, 98, 106, 305, 380, 433, 545, 629, 1037
FalI AAGNNNNNCTT 2 cut(s) 1529, 1561
FaqI GGGAC 4 cut(s) 549, 773, 1489, 1537
FatI CATG 9 cut(s) 39, 94, 102, 301, 376, 429, 541, 625, 1033
FauNDI CATATG 1 cut(s) 1410
FbaI TGATCA 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 108, 367
FokI GGATG 2 cut(s) 299, 1154
Fsp4HI GCNGC 2 cut(s) 108, 367
FspBI CTAG 3 cut(s) 500, 744, 1512
GlaI GCGC 1 cut(s) 838
GluI GCNGC 2 cut(s) 108, 367
GsaI CCCAGC 2 cut(s) 27, 558
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 1 cut(s) 840
HaeIII GGCC 2 cut(s) 375, 390
HapII CCGG 1 cut(s) 1280
HhaI GCGC 1 cut(s) 839
Hin1I GRCGYC 1 cut(s) 837
Hin1II CATG 9 cut(s) 43, 98, 106, 305, 380, 433, 545, 629, 1037
Hin6I GCGC 1 cut(s) 837
HinP1I GCGC 1 cut(s) 837
HincII GTYRAC 2 cut(s) 457, 1428
HindII GTYRAC 2 cut(s) 457, 1428
HindIII AAGCTT 2 cut(s) 986, 993
HinfI GANTC 6 cut(s) 252, 360, 485, 695, 1064, 1276
HpaII CCGG 1 cut(s) 1280
HphI GGTGA 5 cut(s) 181, 515, 1016, 1351, 1425
Hpy166II GTNNAC 3 cut(s) 457, 1321, 1428
Hpy188I TCNGA 7 cut(s) 115, 359, 406, 1138, 1162, 1389, 1507
Hpy188III TCNNGA 7 cut(s) 40, 489, 650, 1147, 1196, 1280, 1382
Hpy8I GTNNAC 3 cut(s) 457, 1321, 1428
HpyAV CCTTC 7 cut(s) 95, 142, 219, 359, 1193, 1349, 1389
HpyCH4III ACNGT 5 cut(s) 454, 571, 805, 943, 1206
HpyCH4IV ACGT 3 cut(s) 459, 867, 1430
HpyCH4V TGCA 3 cut(s) 62, 980, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 104, 372, 1231, 1405, 1497
HpyF3I CTNAG 5 cut(s) 248, 403, 525, 1131, 1504
HpySE526I ACGT 3 cut(s) 459, 867, 1430
Hsp92I GRCGYC 1 cut(s) 837
Hsp92II CATG 9 cut(s) 43, 98, 106, 305, 380, 433, 545, 629, 1037
HspAI GCGC 1 cut(s) 837
KasI GGCGCC 1 cut(s) 836
Kpn2I TCCGGA 1 cut(s) 1279
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
LmnI GCTCC 7 cut(s) 32, 338, 491, 740, 829, 1222, 1282
Lsp1109I GCAGC 2 cut(s) 119, 378
LweI GCATC 2 cut(s) 643, 903
MaeI CTAG 3 cut(s) 500, 744, 1512
MaeII ACGT 3 cut(s) 459, 867, 1430
MaeIII GTNAC 4 cut(s) 187, 289, 799, 1431
MalI GATC 6 cut(s) 8, 117, 445, 891, 1386, 1581
MboI GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
MboII GAAGA 9 cut(s) 80, 431, 476, 719, 949, 1162, 1165, 1382, 1579
MfeI CAATTG 3 cut(s) 72, 1019, 1092
MflI RGATCY 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 8 cut(s) 72, 776, 953, 1019, 1092, 1326, 1342, 1453
MluNI TGGCCA 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 837
MlyI GAGTC 2 cut(s) 494, 704
MmeI TCCRAC 3 cut(s) 320, 559, 1037
Mox20I TGGCCA 1 cut(s) 375
MroI TCCGGA 1 cut(s) 1279
MroXI GAANNNNTTC 1 cut(s) 1068
MscI TGGCCA 1 cut(s) 375
MseI TTAA 2 cut(s) 590, 672
MslI CAYNNNNRTG 4 cut(s) 381, 591, 902, 1438
Msp20I TGGCCA 1 cut(s) 375
MspA1I CMGCKG 2 cut(s) 369, 554
MspI CCGG 1 cut(s) 1280
MspR9I CCNGG 1 cut(s) 1584
MunI CAATTG 3 cut(s) 72, 1019, 1092
MvaI CCWGG 1 cut(s) 1584
MwoI GCNNNNNNNGC 6 cut(s) 68, 104, 372, 1231, 1405, 1497
NarI GGCGCC 1 cut(s) 837
NcoI CCATGG 2 cut(s) 102, 541
NdeI CATATG 1 cut(s) 1410
NdeII GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
NlaIII CATG 9 cut(s) 43, 98, 106, 305, 380, 433, 545, 629, 1037
NlaIV GGNNCC 2 cut(s) 838, 1525
NmuCI GTSAC 4 cut(s) 187, 289, 799, 1431
NspI RCATGY 1 cut(s) 433
OliI CACNNNNGTG 1 cut(s) 1438
PagI TCATGA 1 cut(s) 39
PciI ACATGT 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 4 cut(s) 252, 360, 1064, 1276
PflFI GACNNNGTC 1 cut(s) 1043
PfoI TCCNGGA 1 cut(s) 1582
PkrI GCNGC 2 cut(s) 109, 368
PleI GAGTC 2 cut(s) 493, 703
PluTI GGCGCC 1 cut(s) 840
PpsI GAGTC 2 cut(s) 493, 703
Ppu21I YACGTR 1 cut(s) 868
PscI ACATGT 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 1582
PspFI CCCAGC 2 cut(s) 23, 554
PspGI CCWGG 1 cut(s) 1582
PspN4I GGNNCC 2 cut(s) 838, 1525
PspPI GGNCC 1 cut(s) 1524
PstI CTGCAG 1 cut(s) 1108
PsuI RGATCY 1 cut(s) 115
PsyI GACNNNGTC 1 cut(s) 1043
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 6 cut(s) 866, 1239, 1322, 1334, 1532, 1560
RsaNI GTAC 6 cut(s) 865, 1238, 1321, 1333, 1531, 1559
RseI CAYNNNNRTG 4 cut(s) 381, 591, 902, 1438
SaqAI TTAA 2 cut(s) 590, 672
SatI GCNGC 2 cut(s) 108, 367
Sau3AI GATC 6 cut(s) 6, 115, 443, 889, 1384, 1579
Sau96I GGNCC 1 cut(s) 1524
ScaI AGTACT 3 cut(s) 1239, 1334, 1532
SchI GAGTC 2 cut(s) 494, 704
ScrFI CCNGG 1 cut(s) 1584
SfaNI GCATC 2 cut(s) 643, 903
SfcI CTRYAG 1 cut(s) 1104
SfoI GGCGCC 1 cut(s) 838
SinI GGWCC 1 cut(s) 1524
SmiMI CAYNNNNRTG 4 cut(s) 381, 591, 902, 1438
SnaBI TACGTA 1 cut(s) 868
Sse9I AATT 8 cut(s) 72, 776, 953, 1019, 1092, 1326, 1342, 1453
SsiI CCGC 2 cut(s) 364, 369
SspDI GGCGCC 1 cut(s) 836
SspMI CTAG 3 cut(s) 500, 744, 1512
StyD4I CCNGG 1 cut(s) 1582
StyI CCWWGG 3 cut(s) 102, 541, 1436
TaaI ACNGT 5 cut(s) 454, 571, 805, 943, 1206
TaiI ACGT 3 cut(s) 462, 870, 1433
TaqI TCGA 3 cut(s) 442, 483, 1047
TasI AATT 8 cut(s) 72, 776, 953, 1019, 1092, 1326, 1342, 1453
TatI WGTACW 4 cut(s) 1237, 1320, 1332, 1530
TfiI GAWTC 4 cut(s) 252, 360, 1064, 1276
Tru1I TTAA 2 cut(s) 590, 672
Tru9I TTAA 2 cut(s) 590, 672
TscAI CASTG 2 cut(s) 576, 948
TseFI GTSAC 4 cut(s) 187, 289, 799, 1431
TseI GCWGC 2 cut(s) 107, 366
Tsp45I GTSAC 4 cut(s) 187, 289, 799, 1431
TspDTI ATGAA 9 cut(s) 56, 201, 274, 406, 483, 842, 886, 1434, 1617
TspRI CASTG 2 cut(s) 576, 948
Tth111I GACNNNGTC 1 cut(s) 1043
VpaK11BI GGWCC 1 cut(s) 1524
XapI RAATTY 2 cut(s) 953, 1326
XceI RCATGY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 3 cut(s) 500, 744, 1512
ZrmI AGTACT 3 cut(s) 1239, 1334, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.