MD05G1271700.v1.1

Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004719
Physical Location & Seq
Reverse (-)
40705203 .. 40708937
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1271700.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGCAATTTTCTGTAGGGTGAGATCACTGAATCATCTTTCCAAATGCATTTCCTCAATCGACCCACTTTCTCTTCTGCCATCTTCAACACGCAGATTTTATTCAGAGCAAGTCTCGTCCGGTTTTGAGAGTGTTGGGTTTATAGGGCTGGGAAATATGGGATCAAGGATGGCAAATAATCTAATTAGGGCTGGATACCGAGTCGCTGTTCATGACATAAACAGCAATGCTGTGACGAAGTTCTCTGACATGGGAGCTGCAACCAAAGAAACACCTTTAAAAGTTGCTGAAGCTAGTGATGTTGTAATCACAATGCTGCCTTCCTCATCTCACGTATATGATGTTTATACTGGACCTAATGGTTTACTTCGTGGAGGGGAATTACGACCATGGTTATTAATAGATTCATCCACTATTGATCCACAAACGTCGAGAAAGCTTTCTATTGCTGTATCTGACTGTACTTTAAAGGAGAAAAAAGATTATCCGAAGAAGCCTGCCATGTTGGATGCCCCTGTATCTGGAGGTGTTCTTGCTGCAGAAGCGGGGACTCTTACTTTCATGGTGGGTGGCTCTGAGGAATCTTATCTGGCAGCAAAGTCCTTATTCCTTGCGATGGGCAAGAACATGATTTATTGTGGTGGATCAGGAAATGGTTCAGCAGCAAAGATCTGCAATAATTTGGCAATGTCTATTAGTATGATTGGTGTCTCTGAATCCCTTGCTCTTGGCCAGGCTCTCGGACTTTCCGCCAGTATTCTGACAAGAGTACTCAACTCTTCAAGTGGTCGCTGTTGGAGTAGTGATAGTTATAATCCGGTTCCTGGAGTGATGGAAGGAGTGCCCTCTTCAAGGAACTATGATGGCGGGTTTGCATCCAAACTTATGGCTAAGGATCTAAACCTTGGTGCAACATCAGCCGAAGAGGTTGGTGTTAGATGCCCGTTGACATCCCAAGCACAAAAGATATACACAGAGCTCTGCGAGAATGGTCACGAAACCGAGGACTTCTCCTGTGTTTTCCAGCATTATTACTCCGGCAAGAACGAGAGTTAG

Protein Analysis

352

Amino Acids

37.28

Weight (kDa)

6.53

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 43 - 111 7e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 45 - 214 5.7e-41 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 45 - 107 1.2e-06 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 217 - 342 1.5e-25 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
AciI CCGC 3 cut(s) 545, 750, 867
AclWI GGATC 4 cut(s) 169, 413, 652, 903
AcoI YGGCCR 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 463, 771
AfiI CCNNNNNNNGG 4 cut(s) 521, 616, 824, 852
AgsI TTSAA 3 cut(s) 87, 783, 852
AjnI CCWGG 2 cut(s) 732, 823
AluBI AGCT 4 cut(s) 257, 293, 439, 979
AluI AGCT 4 cut(s) 257, 293, 439, 979
Alw21I GWGCWC 1 cut(s) 981
Alw26I GTCTC 2 cut(s) 118, 715
AlwI GGATC 4 cut(s) 169, 413, 652, 903
AoxI GGCC 1 cut(s) 730
ApeKI GCWGC 5 cut(s) 257, 316, 536, 593, 662
AseI ATTAAT 1 cut(s) 398
Asp700I GAANNNNTTC 2 cut(s) 439, 655
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 846
BalI TGGCCA 1 cut(s) 732
BanII GRGCYC 1 cut(s) 981
Bbv12I GWGCWC 1 cut(s) 981
BbvI GCAGC 5 cut(s) 244, 303, 523, 605, 674
BccI CCATC 5 cut(s) 88, 163, 610, 826, 857
BciT130I CCWGG 2 cut(s) 734, 825
BciVI GTATCC 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 118, 715
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 2 cut(s) 13, 537
BfuI GTATCC 1 cut(s) 188
BglII AGATCT 1 cut(s) 669
BisI GCNGC 5 cut(s) 258, 317, 537, 594, 663
BlsI GCNGC 5 cut(s) 259, 318, 538, 595, 664
BmcAI AGTACT 1 cut(s) 771
Bme1390I CCNGG 2 cut(s) 734, 825
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 822
BmrFI CCNGG 2 cut(s) 734, 825
BmsI GCATC 3 cut(s) 499, 884, 929
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 995, 1027
BpmI CTGGAG 2 cut(s) 543, 846
Bpu10I CCTNAGC 1 cut(s) 891
BsaAI YACGTR 1 cut(s) 334
BsaJI CCNNGG 3 cut(s) 389, 904, 1002
BsaWI WCCGGW 2 cut(s) 119, 817
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 616, 824, 852
Bse1I ACTGG 2 cut(s) 355, 753
Bse3DI GCAATG 2 cut(s) 232, 693
BseBI CCWGG 2 cut(s) 734, 825
BseDI CCNNGG 3 cut(s) 389, 904, 1002
BseGI GGATG 5 cut(s) 174, 407, 514, 875, 950
BseLI CCNNNNNNNGG 4 cut(s) 521, 616, 824, 852
BseMI GCAATG 2 cut(s) 232, 693
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 2 cut(s) 355, 753
BseSI GKGCMC 1 cut(s) 846
BseXI GCAGC 5 cut(s) 244, 303, 523, 605, 674
BseYI CCCAGC 1 cut(s) 148
BshFI GGCC 1 cut(s) 732
BsiHKAI GWGCWC 1 cut(s) 981
BsiSI CCGG 3 cut(s) 120, 818, 1038
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 4 cut(s) 521, 616, 824, 852
BsmAI GTCTC 2 cut(s) 118, 715
BsmFI GGGAC 1 cut(s) 562
BsnI GGCC 1 cut(s) 732
Bsp1286I GDGCHC 2 cut(s) 846, 981
Bsp143I GATC 6 cut(s) 23, 161, 418, 644, 669, 895
Bsp19I CCATGG 1 cut(s) 389
BspACI CCGC 3 cut(s) 545, 750, 867
BspANI GGCC 1 cut(s) 732
BspCNI CTCAG 1 cut(s) 568
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 822
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 4 cut(s) 169, 413, 652, 903
BsrDI GCAATG 2 cut(s) 232, 693
BsrI ACTGG 2 cut(s) 355, 753
BssECI CCNNGG 3 cut(s) 389, 904, 1002
BssMI GATC 6 cut(s) 23, 161, 418, 644, 669, 895
BssT1I CCWWGG 2 cut(s) 389, 904
Bst2UI CCWGG 2 cut(s) 734, 825
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 4 cut(s) 78, 784, 853, 918
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 576, 891
BstDSI CCRYGG 1 cut(s) 389
BstENI CCTNNNNNAGG 1 cut(s) 850
BstF5I GGATG 5 cut(s) 174, 407, 514, 875, 950
BstKTI GATC 6 cut(s) 26, 164, 421, 647, 672, 898
BstMAI GTCTC 2 cut(s) 118, 715
BstMBI GATC 6 cut(s) 23, 161, 418, 644, 669, 895
BstMWI GCNNNNNNNGC 2 cut(s) 542, 917
BstNI CCWGG 2 cut(s) 734, 825
BstSCI CCNGG 2 cut(s) 732, 823
BstSFI CTRYAG 2 cut(s) 13, 537
BstSLI GKGCMC 1 cut(s) 846
BstV1I GCAGC 5 cut(s) 244, 303, 523, 605, 674
BstX2I RGATCY 2 cut(s) 669, 895
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 2 cut(s) 669, 895
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 629
BtsCI GGATG 5 cut(s) 174, 407, 514, 875, 950
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 498
CciI TCATGA 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 353
Csp6I GTAC 2 cut(s) 462, 770
CviAII CATG 6 cut(s) 212, 250, 390, 502, 562, 628
CviQI GTAC 2 cut(s) 462, 770
DdeI CTNAG 2 cut(s) 576, 891
DpnI GATC 6 cut(s) 25, 163, 420, 646, 671, 897
DpnII GATC 6 cut(s) 23, 161, 418, 644, 669, 895
DraI TTTAAA 2 cut(s) 279, 468
EaeI YGGCCR 1 cut(s) 730
Eam1104I CTCTTC 4 cut(s) 78, 784, 853, 918
EarI CTCTTC 4 cut(s) 78, 784, 853, 918
EciI GGCGGA 1 cut(s) 739
Ecl136II GAGCTC 1 cut(s) 979
Eco130I CCWWGG 2 cut(s) 389, 904
Eco24I GRGCYC 1 cut(s) 981
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 979
Eco57I CTGAAG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 979
EcoNI CCTNNNNNAGG 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 732, 823
EcoT14I CCWWGG 2 cut(s) 389, 904
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 981
ErhI CCWWGG 2 cut(s) 389, 904
FaeI CATG 6 cut(s) 215, 253, 393, 505, 565, 631
FaqI GGGAC 1 cut(s) 562
FatI CATG 6 cut(s) 211, 249, 389, 501, 561, 627
FauI CCCGC 2 cut(s) 538, 860
Fnu4HI GCNGC 5 cut(s) 258, 317, 537, 594, 663
FokI GGATG 5 cut(s) 181, 394, 521, 862, 937
FriOI GRGCYC 1 cut(s) 981
Fsp4HI GCNGC 5 cut(s) 258, 317, 537, 594, 663
FspBI CTAG 1 cut(s) 294
GluI GCNGC 5 cut(s) 258, 317, 537, 594, 663
GsaI CCCAGC 1 cut(s) 152
GsuI CTGGAG 2 cut(s) 543, 846
HaeIII GGCC 1 cut(s) 732
HapII CCGG 3 cut(s) 120, 818, 1038
Hin1II CATG 6 cut(s) 215, 253, 393, 505, 565, 631
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 6 cut(s) 31, 201, 404, 550, 581, 716
HpaII CCGG 3 cut(s) 120, 818, 1038
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 365, 948
Hpy188I TCNGA 8 cut(s) 106, 247, 457, 489, 577, 715, 743, 762
Hpy188III TCNNGA 5 cut(s) 212, 432, 522, 648, 995
Hpy8I GTNNAC 2 cut(s) 365, 948
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 2 cut(s) 330, 830
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 2 cut(s) 333, 428
HpyCH4V TGCA 6 cut(s) 48, 260, 539, 675, 875, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 917
HpyF3I CTNAG 2 cut(s) 576, 891
HpySE526I ACGT 2 cut(s) 333, 428
Hsp92II CATG 6 cut(s) 215, 253, 393, 505, 565, 631
Kzo9I GATC 6 cut(s) 23, 161, 418, 644, 669, 895
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 5 cut(s) 244, 303, 523, 605, 674
LweI GCATC 3 cut(s) 499, 884, 929
MaeI CTAG 1 cut(s) 294
MaeII ACGT 2 cut(s) 333, 428
MaeIII GTNAC 2 cut(s) 232, 992
MalI GATC 6 cut(s) 25, 163, 420, 646, 671, 897
MboI GATC 6 cut(s) 23, 161, 418, 644, 669, 895
MboII GAAGA 6 cut(s) 65, 75, 502, 771, 840, 935
MflI RGATCY 2 cut(s) 669, 895
MhlI GDGCHC 2 cut(s) 846, 981
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 4 cut(s) 6, 183, 380, 679
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 2 cut(s) 210, 544
MmeI TCCRAC 2 cut(s) 486, 776
MnlI CCTC 8 cut(s) 64, 334, 368, 518, 571, 856, 919, 997
Mox20I TGGCCA 1 cut(s) 732
Mph1103I ATGCAT 1 cut(s) 50
MroXI GAANNNNTTC 2 cut(s) 439, 655
MscI TGGCCA 1 cut(s) 732
MseI TTAA 3 cut(s) 278, 398, 467
MslI CAYNNNNRTG 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 120, 818, 1038
MspR9I CCNGG 2 cut(s) 734, 825
MvaI CCWGG 2 cut(s) 734, 825
MwoI GCNNNNNNNGC 2 cut(s) 542, 917
NcoI CCATGG 1 cut(s) 389
NdeII GATC 6 cut(s) 23, 161, 418, 644, 669, 895
NlaIII CATG 6 cut(s) 215, 253, 393, 505, 565, 631
NlaIV GGNNCC 1 cut(s) 822
NmuCI GTSAC 2 cut(s) 232, 992
NsiI ATGCAT 1 cut(s) 50
PagI TCATGA 1 cut(s) 211
PdmI GAANNNNTTC 2 cut(s) 439, 655
PfeI GAWTC 4 cut(s) 31, 404, 581, 716
PfoI TCCNGGA 1 cut(s) 823
PkrI GCNGC 5 cut(s) 259, 318, 538, 595, 664
PleI GAGTC 2 cut(s) 209, 544
PpsI GAGTC 2 cut(s) 209, 544
Ppu21I YACGTR 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 398
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 981
Psp6I CCWGG 2 cut(s) 732, 823
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 2 cut(s) 732, 823
PspN4I GGNNCC 1 cut(s) 822
PspPI GGNCC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 2 cut(s) 669, 895
RsaI GTAC 2 cut(s) 463, 771
RsaNI GTAC 2 cut(s) 462, 770
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 1 cut(s) 981
SaqAI TTAA 3 cut(s) 278, 398, 467
SatI GCNGC 5 cut(s) 258, 317, 537, 594, 663
Sau3AI GATC 6 cut(s) 23, 161, 418, 644, 669, 895
Sau96I GGNCC 1 cut(s) 353
ScaI AGTACT 1 cut(s) 771
SchI GAGTC 2 cut(s) 210, 544
ScrFI CCNGG 2 cut(s) 734, 825
SduI GDGCHC 2 cut(s) 846, 981
SfaNI GCATC 3 cut(s) 499, 884, 929
SfcI CTRYAG 2 cut(s) 13, 537
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 336
Sse9I AATT 4 cut(s) 6, 183, 380, 679
SsiI CCGC 3 cut(s) 545, 750, 867
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 981
StyD4I CCNGG 2 cut(s) 732, 823
StyI CCWWGG 2 cut(s) 389, 904
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 2 cut(s) 336, 431
TaqI TCGA 2 cut(s) 60, 431
TasI AATT 4 cut(s) 6, 183, 380, 679
TatI WGTACW 2 cut(s) 461, 769
TfiI GAWTC 4 cut(s) 31, 404, 581, 716
Tru1I TTAA 3 cut(s) 278, 398, 467
Tru9I TTAA 3 cut(s) 278, 398, 467
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 2 cut(s) 232, 992
TseI GCWGC 5 cut(s) 257, 316, 536, 593, 662
Tsp45I GTSAC 2 cut(s) 232, 992
TspDTI ATGAA 3 cut(s) 200, 396, 550
TspRI CASTG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 353
VspI ATTAAT 1 cut(s) 398
XagI CCTNNNNNAGG 1 cut(s) 850
XmnI GAANNNNTTC 2 cut(s) 439, 655
XspI CTAG 1 cut(s) 294
ZrmI AGTACT 1 cut(s) 771
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.