Rh5BG124900

Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004719
Physical Location & Seq
Forward (+)
11807580 .. 11811050
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG124900.1

Sequence Viewer

Length: 795 bp
ATGGCAGTCTTGTACAGAGTGAGATCACTACATCATCTATCCAAATGTATTTCCACAAAAATTTTACCGAGGAGATTTTACTCGGCACAAGTCTCGTCCCAATTTGAGACTGTAGGGTTTATTGGACTGGGAAATATGGGATCCAGAATGGCAAACAATCTAATAAAGGCGGGATACCGAGTAGCTGTTCATGACATAAACTGCAATGCCATGAAAAAGTTCTCTGATATTGGAGCCGCAACAAAAGAAACACCTACTGAAGTTGCAGAAGCTAGTGATGTTGTAATCACAATGCTCCCTACATCATCTCATGTATATGATGTTTATACTGGACCAAATGGTTTACTTTATGGAGGGAATTTGCGACCACGGCTATTTATAGATTCATCCACTATTGATCCACAAACATCGAGAAAGCTTTCTACCATCGTCTCTAACTGTACTCTAAAGGAACAGAAAGAAGATCATTGGAAGAAGCCTGCCTTGCTGGATGCTCCTGTATCTGGAGGTGTCCTTGCAGCGGAAGCTGGGACTCTTACTTTCATGGTTGGTGGATCTGAGGAAGCTTATATGGCGGCTGAACCCTTGTTCCTTTCGATGGGGAAGAACACAGTTTATTGTCGTGGGGCAGGAAATGGTTCAGCAGCAAAGATTTGCAATAATCTGGCACTGGCTATAAGTATGCTTGGCGTCTCAGAATCTCTTGCTCTTGGTCAGTCTCTAGGAATATCTGCCAGTACTCTAACAAAAGTGTTTAACTCTTCCAGTGCTCGCTGTTGGAGCAGGTATTCTTGA

Protein Analysis

264

Amino Acids

28.25

Weight (kDa)

9.11

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 4 - 104 5.6e-09 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 37 - 206 1.4e-41 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 37 - 101 2.7e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 211 - 261 7.5e-11 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
AciI CCGC 4 cut(s) 170, 237, 521, 575
AclWI GGATC 4 cut(s) 135, 148, 392, 562
AcsI RAATTY 2 cut(s) 60, 358
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 690
AfaI GTAC 3 cut(s) 14, 442, 739
AfiI CCNNNNNNNGG 3 cut(s) 503, 520, 598
AloI GAACNNNNNNTCC 2 cut(s) 573, 605
AluBI AGCT 5 cut(s) 185, 272, 418, 527, 566
AluI AGCT 5 cut(s) 185, 272, 418, 527, 566
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 5 cut(s) 97, 101, 436, 697, 723
AlwI GGATC 4 cut(s) 135, 148, 392, 562
ApeKI GCWGC 2 cut(s) 518, 644
ApoI RAATTY 2 cut(s) 60, 358
Asp700I GAANNNNTTC 3 cut(s) 218, 418, 637
AspS9I GGNCC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 332
BamHI GGATCC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 2 cut(s) 530, 656
BccI CCATC 2 cut(s) 434, 592
BceAI ACGGC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BciVI GTATCC 1 cut(s) 167
BcoDI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BfaI CTAG 2 cut(s) 273, 722
BfmI CTRYAG 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 774
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 237, 519, 576, 645
BlsI GCNGC 4 cut(s) 238, 520, 577, 646
BmcAI AGTACT 1 cut(s) 739
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 142, 235
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 690
BsaJI CCNNGG 2 cut(s) 68, 368
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 520, 598
Bse1I ACTGG 5 cut(s) 132, 334, 675, 735, 765
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 68, 368
BseGI GGATG 2 cut(s) 386, 496
BseLI CCNNNNNNNGG 3 cut(s) 503, 520, 598
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 549, 708
BseNI ACTGG 5 cut(s) 132, 334, 675, 735, 765
BseRI GAGGAG 1 cut(s) 85
BseXI GCAGC 2 cut(s) 530, 656
BseYI CCCAGC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 772
BslFI GGGAC 2 cut(s) 82, 544
BslI CCNNNNNNNGG 3 cut(s) 503, 520, 598
BsmAI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BsmBI CGTCTC 2 cut(s) 436, 697
BsmFI GGGAC 2 cut(s) 82, 544
Bsp1286I GDGCHC 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 5 cut(s) 23, 140, 397, 463, 554
BspACI CCGC 4 cut(s) 170, 237, 521, 575
BspCNI CTCAG 2 cut(s) 550, 707
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 142, 235
BspMI ACCTGC 1 cut(s) 774
BspPI GGATC 4 cut(s) 135, 148, 392, 562
BsrDI GCAATG 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 5 cut(s) 132, 334, 675, 735, 765
BssECI CCNNGG 2 cut(s) 68, 368
BssMI GATC 5 cut(s) 23, 140, 397, 463, 554
BssNI GRCGYC 1 cut(s) 690
Bst4CI ACNGT 3 cut(s) 112, 440, 613
Bst6I CTCTTC 1 cut(s) 766
BstACI GRCGYC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 480, 772
BstDEI CTNAG 2 cut(s) 558, 694
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 2 cut(s) 386, 496
BstKTI GATC 5 cut(s) 26, 143, 400, 466, 557
BstMAI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BstMBI GATC 5 cut(s) 23, 140, 397, 463, 554
BstMWI GCNNNNNNNGC 5 cut(s) 370, 484, 524, 572, 780
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 530, 656
BstX2I RGATCY 2 cut(s) 140, 554
BstYI RGATCY 2 cut(s) 140, 554
BsuI GTATCC 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 2 cut(s) 386, 496
BtsIMutI CAGTG 2 cut(s) 668, 772
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 2 cut(s) 480, 772
CciI TCATGA 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 332
CseI GACGC 1 cut(s) 679
Csp6I GTAC 3 cut(s) 13, 441, 738
CviAII CATG 4 cut(s) 191, 211, 311, 544
CviQI GTAC 3 cut(s) 13, 441, 738
DdeI CTNAG 2 cut(s) 558, 694
DpnI GATC 5 cut(s) 25, 142, 399, 465, 556
DpnII GATC 5 cut(s) 23, 140, 397, 463, 554
Eam1104I CTCTTC 1 cut(s) 766
EarI CTCTTC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 279
Esp3I CGTCTC 2 cut(s) 436, 697
FaeI CATG 4 cut(s) 194, 214, 314, 547
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 82, 544
FatI CATG 4 cut(s) 190, 210, 310, 543
FauI CCCGC 1 cut(s) 163
Fnu4HI GCNGC 4 cut(s) 237, 519, 576, 645
FokI GGATG 2 cut(s) 373, 503
Fsp4HI GCNGC 4 cut(s) 237, 519, 576, 645
FspBI CTAG 2 cut(s) 273, 722
GluI GCNGC 4 cut(s) 237, 519, 576, 645
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 525
HgaI GACGC 1 cut(s) 679
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 4 cut(s) 194, 214, 314, 547
HindIII AAGCTT 2 cut(s) 416, 564
HinfI GANTC 3 cut(s) 383, 532, 698
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 3 cut(s) 226, 559, 697
Hpy188III TCNNGA 5 cut(s) 144, 191, 411, 504, 792
Hpy8I GTNNAC 1 cut(s) 344
HpyCH4III ACNGT 3 cut(s) 112, 440, 613
HpyCH4V TGCA 4 cut(s) 204, 266, 518, 657
HpyF10VI GCNNNNNNNGC 5 cut(s) 370, 484, 524, 572, 780
HpyF3I CTNAG 2 cut(s) 558, 694
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 4 cut(s) 194, 214, 314, 547
Kzo9I GATC 5 cut(s) 23, 140, 397, 463, 554
LmnI GCTCC 4 cut(s) 233, 300, 499, 780
Lsp1109I GCAGC 2 cut(s) 530, 656
LweI GCATC 1 cut(s) 481
MaeI CTAG 2 cut(s) 273, 722
MalI GATC 5 cut(s) 25, 142, 399, 465, 556
MboI GATC 5 cut(s) 23, 140, 397, 463, 554
MboII GAAGA 4 cut(s) 473, 484, 616, 753
MflI RGATCY 2 cut(s) 140, 554
MhlI GDGCHC 1 cut(s) 772
MluCI AATT 3 cut(s) 60, 101, 358
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 4 cut(s) 63, 347, 500, 553
MroXI GAANNNNTTC 3 cut(s) 218, 418, 637
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 521
MwoI GCNNNNNNNGC 5 cut(s) 370, 484, 524, 572, 780
NdeII GATC 5 cut(s) 23, 140, 397, 463, 554
NlaIII CATG 4 cut(s) 194, 214, 314, 547
NlaIV GGNNCC 2 cut(s) 142, 235
NmeAIII GCCGAG 1 cut(s) 62
PagI TCATGA 1 cut(s) 190
PdmI GAANNNNTTC 3 cut(s) 218, 418, 637
PfeI GAWTC 2 cut(s) 383, 698
PkrI GCNGC 4 cut(s) 238, 520, 577, 646
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 527
PspN4I GGNNCC 2 cut(s) 142, 235
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 2 cut(s) 140, 554
RsaI GTAC 3 cut(s) 14, 442, 739
RsaNI GTAC 3 cut(s) 13, 441, 738
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 4 cut(s) 237, 519, 576, 645
Sau3AI GATC 5 cut(s) 23, 140, 397, 463, 554
Sau96I GGNCC 1 cut(s) 332
ScaI AGTACT 1 cut(s) 739
SchI GAGTC 1 cut(s) 526
SduI GDGCHC 1 cut(s) 772
SetI ASST 8 cut(s) 187, 256, 274, 420, 511, 529, 568, 788
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 3 cut(s) 60, 101, 358
SsiI CCGC 4 cut(s) 170, 237, 521, 575
SspMI CTAG 2 cut(s) 273, 722
TaaI ACNGT 3 cut(s) 112, 440, 613
TaqI TCGA 2 cut(s) 410, 596
TasI AATT 3 cut(s) 60, 101, 358
TatI WGTACW 3 cut(s) 12, 440, 737
TauI GCSGC 2 cut(s) 239, 578
TfiI GAWTC 2 cut(s) 383, 698
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 2 cut(s) 675, 772
TseI GCWGC 2 cut(s) 518, 644
TspDTI ATGAA 4 cut(s) 179, 227, 375, 532
TspRI CASTG 2 cut(s) 675, 772
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 2 cut(s) 60, 358
XmnI GAANNNNTTC 3 cut(s) 218, 418, 637
XspI CTAG 2 cut(s) 273, 722
ZrmI AGTACT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.