RchiOBHm_Chr5g0016441

Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004719
Physical Location & Seq
Forward (+)
11302611 .. 11306352
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29681

Sequence Viewer

Length: 1038 bp
ATGGCAGTCTTGTACAGAGTGAGATCAATACATCATCTATCCAAATGTATTTCCACAAAAATTTTACCGGGGAGATTTTACTCGGCACAAGTCTCGTCCCAATTTGAGACTGTAGGGTTTATTGGACTGGGAAATATGGGATCCAGAATGGCAAACAATCTAATAAAGGCGGGATACCGAGTAGCTGTTCATGACATAAACTGCAATGCCATGAAAAAGTTCTCTGATATTGGAGCCGCAACAAAAGAAACACCTACTGAAGTTGCAGAAGCTAGTGATGTTGTAATCACAATGCTCCCTACATCATCTCATGTATATGATGTTTATACTGGACCAAATGGTTTACTTTATGGAGGGAATTTGCGACCACGGCTATTTATAGATTCATCCACTATTGATCCACAAACATCGAGAAAGCTTTCTACCATCGTCTCTAACTGTACTCTAAAGGAACAGAAAGAAGATCATTGGAAGAAGCCTGCCTTGCTGGATGCTCCAGTATCTGGAGGTGTCCTTGCAGCGGAAGCTGGGACTCTTACTTTCATGGTTGGTGGATCTGAGGAAGCTTATATGGCGGCTGAACCCTTGTTCCTTTCGATGGGGAAGAACACAGTTTATTGTGGTGGGGCAGGAAATGGTTCAGCAGCAAAGATTTGCAATAATCTGGCACTGGCTATAAGTATGCTTGGCGTCTCAGAATCTCTTGCTCTTGGTCAGTCTCTAGGAATATCTGCCAGTACTCTAACAAAAGTGTTTAACTCTTCCAGTGCTCGCTGTTGGAGCAGCGATAGTTACAATCCAGTTCCGGGAGTGATGGAAGGGGTTCCCTCTTCGAGAAACTATGATGGTGGGTTTGCATCCAAACTAATGGCTAAAGACGTAAATATTGCTGCAACATCAGCCAAAGAGGTTGGTCTTCGATGCCCGTTGACTTCCCAAGCACAAGAGATATATACAGAGCTCTGTGAGGATGGCCATGAAACCAAGGACTTCTCCTGCGTATTCCGCCATTATTACTCTGGCAAGAATGAGATTTAG

Protein Analysis

345

Amino Acids

36.84

Weight (kDa)

7.02

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 4 - 104 1.2e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 37 - 208 1.2e-41 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 37 - 101 4.4e-07 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_11 PF14833 211 - 337 2.3e-27 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 503
AciI CCGC 5 cut(s) 170, 237, 521, 575, 1006
AclWI GGATC 4 cut(s) 135, 148, 392, 562
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 2 cut(s) 60, 358
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 690
AfaI GTAC 3 cut(s) 14, 442, 739
AfiI CCNNNNNNNGG 4 cut(s) 503, 520, 598, 806
AloI GAACNNNNNNTCC 2 cut(s) 573, 605
AluBI AGCT 6 cut(s) 185, 272, 418, 527, 566, 961
AluI AGCT 6 cut(s) 185, 272, 418, 527, 566, 961
Alw21I GWGCWC 2 cut(s) 772, 963
Alw26I GTCTC 5 cut(s) 97, 101, 436, 697, 723
AlwI GGATC 4 cut(s) 135, 148, 392, 562
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 1 cut(s) 973
ApeKI GCWGC 4 cut(s) 518, 644, 783, 890
ApoI RAATTY 2 cut(s) 60, 358
Asp700I GAANNNNTTC 4 cut(s) 218, 418, 637, 822
AspS9I GGNCC 1 cut(s) 332
AsuC2I CCSGG 2 cut(s) 69, 807
AvaII GGWCC 1 cut(s) 332
BalI TGGCCA 1 cut(s) 975
BamHI GGATCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 2 cut(s) 772, 963
BbvI GCAGC 4 cut(s) 530, 656, 795, 877
BccI CCATC 5 cut(s) 434, 592, 808, 839, 965
BceAI ACGGC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 2 cut(s) 69, 807
BcoDI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BfaI CTAG 2 cut(s) 273, 722
BfmI CTRYAG 1 cut(s) 111
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 6 cut(s) 237, 519, 576, 645, 784, 891
BlsI GCNGC 6 cut(s) 238, 520, 577, 646, 785, 892
BmcAI AGTACT 1 cut(s) 739
Bme1390I CCNGG 2 cut(s) 69, 807
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 3 cut(s) 142, 235, 825
BmrFI CCNGG 2 cut(s) 69, 807
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 3 cut(s) 481, 866, 911
BmuI ACTGGG 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 908
BpmI CTGGAG 2 cut(s) 480, 525
BpuMI CCSGG 2 cut(s) 69, 807
BsaHI GRCGYC 1 cut(s) 690
BsaJI CCNNGG 3 cut(s) 68, 368, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 503, 520, 598, 806
Bse1I ACTGG 7 cut(s) 132, 334, 497, 675, 735, 765, 800
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 3 cut(s) 68, 368, 984
BseGI GGATG 4 cut(s) 386, 496, 857, 976
BseLI CCNNNNNNNGG 4 cut(s) 503, 520, 598, 806
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 549, 708
BseNI ACTGG 7 cut(s) 132, 334, 497, 675, 735, 765, 800
BseXI GCAGC 4 cut(s) 530, 656, 795, 877
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 975
BsiHKAI GWGCWC 2 cut(s) 772, 963
BsiSI CCGG 2 cut(s) 68, 806
BslFI GGGAC 2 cut(s) 82, 544
BslI CCNNNNNNNGG 4 cut(s) 503, 520, 598, 806
BsmAI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BsmBI CGTCTC 2 cut(s) 436, 697
BsmFI GGGAC 2 cut(s) 82, 544
BsnI GGCC 1 cut(s) 975
Bsp1286I GDGCHC 2 cut(s) 772, 963
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 5 cut(s) 23, 140, 397, 463, 554
BspACI CCGC 5 cut(s) 170, 237, 521, 575, 1006
BspANI GGCC 1 cut(s) 975
BspCNI CTCAG 2 cut(s) 550, 707
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 3 cut(s) 142, 235, 825
BspPI GGATC 4 cut(s) 135, 148, 392, 562
BsrDI GCAATG 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 7 cut(s) 132, 334, 497, 675, 735, 765, 800
BssECI CCNNGG 3 cut(s) 68, 368, 984
BssMI GATC 5 cut(s) 23, 140, 397, 463, 554
BssNI GRCGYC 1 cut(s) 690
BssT1I CCWWGG 1 cut(s) 984
Bst4CI ACNGT 3 cut(s) 112, 440, 613
Bst6I CTCTTC 2 cut(s) 766, 835
BstACI GRCGYC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 480, 772
BstDEI CTNAG 2 cut(s) 558, 694
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 4 cut(s) 386, 496, 857, 976
BstKTI GATC 5 cut(s) 26, 143, 400, 466, 557
BstMAI GTCTC 5 cut(s) 97, 101, 436, 697, 723
BstMBI GATC 5 cut(s) 23, 140, 397, 463, 554
BstMWI GCNNNNNNNGC 7 cut(s) 370, 484, 524, 572, 780, 899, 1005
BstSCI CCNGG 2 cut(s) 67, 805
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 4 cut(s) 530, 656, 795, 877
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 2 cut(s) 140, 554
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 2 cut(s) 140, 554
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 4 cut(s) 386, 496, 857, 976
BtsIMutI CAGTG 2 cut(s) 668, 772
Cac8I GCNNGC 2 cut(s) 480, 772
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 332
CseI GACGC 1 cut(s) 679
Csp6I GTAC 3 cut(s) 13, 441, 738
CviAII CATG 5 cut(s) 191, 211, 311, 544, 977
CviQI GTAC 3 cut(s) 13, 441, 738
DdeI CTNAG 2 cut(s) 558, 694
DpnI GATC 5 cut(s) 25, 142, 399, 465, 556
DpnII GATC 5 cut(s) 23, 140, 397, 463, 554
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 2 cut(s) 766, 835
EarI CTCTTC 2 cut(s) 766, 835
EciI GGCGGA 1 cut(s) 995
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 984
EcoT38I GRGCYC 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 984
Esp3I CGTCTC 2 cut(s) 436, 697
FaeI CATG 5 cut(s) 194, 214, 314, 547, 980
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 82, 544
FatI CATG 5 cut(s) 190, 210, 310, 543, 976
FauI CCCGC 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 237, 519, 576, 645, 784, 891
FokI GGATG 4 cut(s) 373, 503, 844, 983
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 6 cut(s) 237, 519, 576, 645, 784, 891
FspBI CTAG 2 cut(s) 273, 722
GluI GCNGC 6 cut(s) 237, 519, 576, 645, 784, 891
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 2 cut(s) 480, 525
HaeIII GGCC 1 cut(s) 975
HapII CCGG 2 cut(s) 68, 806
HgaI GACGC 1 cut(s) 679
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 5 cut(s) 194, 214, 314, 547, 980
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 2 cut(s) 416, 564
HinfI GANTC 3 cut(s) 383, 532, 698
HpaII CCGG 2 cut(s) 68, 806
Hpy166II GTNNAC 2 cut(s) 344, 930
Hpy188I TCNGA 3 cut(s) 226, 559, 697
Hpy188III TCNNGA 5 cut(s) 144, 191, 411, 504, 834
Hpy8I GTNNAC 2 cut(s) 344, 930
HpyAV CCTTC 1 cut(s) 812
HpyCH4III ACNGT 3 cut(s) 112, 440, 613
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 6 cut(s) 204, 266, 518, 657, 857, 893
HpyF10VI GCNNNNNNNGC 7 cut(s) 370, 484, 524, 572, 780, 899, 1005
HpyF3I CTNAG 2 cut(s) 558, 694
HpySE526I ACGT 1 cut(s) 879
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 5 cut(s) 194, 214, 314, 547, 980
Kzo9I GATC 5 cut(s) 23, 140, 397, 463, 554
LmnI GCTCC 4 cut(s) 233, 300, 499, 780
Lsp1109I GCAGC 4 cut(s) 530, 656, 795, 877
LweI GCATC 3 cut(s) 481, 866, 911
MaeI CTAG 2 cut(s) 273, 722
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 1 cut(s) 791
MalI GATC 5 cut(s) 25, 142, 399, 465, 556
MboI GATC 5 cut(s) 23, 140, 397, 463, 554
MboII GAAGA 6 cut(s) 473, 484, 616, 753, 822, 908
MflI RGATCY 2 cut(s) 140, 554
MhlI GDGCHC 2 cut(s) 772, 963
MlsI TGGCCA 1 cut(s) 975
MluCI AATT 3 cut(s) 60, 101, 358
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 6 cut(s) 347, 500, 553, 838, 901, 961
Mox20I TGGCCA 1 cut(s) 975
MroXI GAANNNNTTC 4 cut(s) 218, 418, 637, 822
MscI TGGCCA 1 cut(s) 975
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 975
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 68, 806
MspR9I CCNGG 2 cut(s) 69, 807
MwoI GCNNNNNNNGC 7 cut(s) 370, 484, 524, 572, 780, 899, 1005
NciI CCSGG 2 cut(s) 69, 807
NdeII GATC 5 cut(s) 23, 140, 397, 463, 554
NlaIII CATG 5 cut(s) 194, 214, 314, 547, 980
NlaIV GGNNCC 3 cut(s) 142, 235, 825
NmeAIII GCCGAG 1 cut(s) 62
PagI TCATGA 1 cut(s) 190
PdmI GAANNNNTTC 4 cut(s) 218, 418, 637, 822
PfeI GAWTC 2 cut(s) 383, 698
PflMI CCANNNNNTGG 1 cut(s) 503
PfoI TCCNGGA 1 cut(s) 805
PkrI GCNGC 6 cut(s) 238, 520, 577, 646, 785, 892
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp124BI GAGCTC 1 cut(s) 963
PspFI CCCAGC 1 cut(s) 527
PspN4I GGNNCC 3 cut(s) 142, 235, 825
PspPI GGNCC 1 cut(s) 332
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 2 cut(s) 140, 554
RsaI GTAC 3 cut(s) 14, 442, 739
RsaNI GTAC 3 cut(s) 13, 441, 738
RseI CAYNNNNRTG 1 cut(s) 315
SacI GAGCTC 1 cut(s) 963
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 6 cut(s) 237, 519, 576, 645, 784, 891
Sau3AI GATC 5 cut(s) 23, 140, 397, 463, 554
Sau96I GGNCC 1 cut(s) 332
ScaI AGTACT 1 cut(s) 739
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 2 cut(s) 69, 807
SduI GDGCHC 2 cut(s) 772, 963
SfaNI GCATC 3 cut(s) 481, 866, 911
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 3 cut(s) 60, 101, 358
SsiI CCGC 5 cut(s) 170, 237, 521, 575, 1006
SspI AATATT 1 cut(s) 886
SspMI CTAG 2 cut(s) 273, 722
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 2 cut(s) 67, 805
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 3 cut(s) 112, 440, 613
TaiI ACGT 1 cut(s) 882
TaqI TCGA 4 cut(s) 410, 596, 833, 919
TasI AATT 3 cut(s) 60, 101, 358
TatI WGTACW 3 cut(s) 12, 440, 737
TauI GCSGC 2 cut(s) 239, 578
TfiI GAWTC 2 cut(s) 383, 698
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 2 cut(s) 675, 772
TseI GCWGC 4 cut(s) 518, 644, 783, 890
TspDTI ATGAA 5 cut(s) 179, 227, 375, 532, 993
TspRI CASTG 2 cut(s) 675, 772
Van91I CCANNNNNTGG 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 2 cut(s) 60, 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
XmnI GAANNNNTTC 4 cut(s) 218, 418, 637, 822
XspI CTAG 2 cut(s) 273, 722
ZrmI AGTACT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.