Rw5G010910

Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004719
Physical Location & Seq
Forward (+)
12019123 .. 12022702
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010910.1

Sequence Viewer

Length: 744 bp
ATGGCGGTCTTGTACAGAGTGAGATCACTACATCATCTATCCAAATGTATTTCCACAAAAATTTTACCGGGGAGATTTTACTCGGCACAAGTCTCGTCCCAATTTGAGACTGTAGGGTTTATTGGACTGGGAAATATGGGATCCAGAATGGCAAACAATCTAATAAAGGCCGGATACCGAGTAGCTGTTCATGACATAAACTGCAATGCCATGAAAAAGTTCTCTGATATTGGAGCCGCAACAAAAGAAACACCTACTGAAGTTGCAGAAGCTAGTGATGTTGTAATCACAATGCTCCCTACATCATCTCATGTATATGATGTTTATACTGGACCAAATGGTTTACTTTATGGAGGGAATTTGCGACCACGGCTATTTATAGATTCATCCACTATTGATCCACAAACATCGAGAAAGCTTTCTACCATCGTCTCTAACTGTACTCTAAAGGAACAGAAAGAAGATCATTGGAAGAAGCCTGCCTTGCTGGATGCTCCTGTATCTGGAGGTGTCCTTGCAGCGGAAGCTGGGACTCTTACTTTCATGGTTGGTGGATCTGAGGAAGCTTATATGGCGGCTGAACCCTTGTTCCTTTCGATGGGGAAGAACACAGTTTATTGTGGTGGGGCAGGAAATGGTTCAGTAAGTTCCATCATTACTGCATCAAATATACATTATATTCAGCTTATTGTGAACTTTTCATGCTTAGTTCTTAGATGTCCTGAATTTGCTGCTGAGTATTAG

Protein Analysis

247

Amino Acids

26.59

Weight (kDa)

7.62

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 7 - 104 7.6e-09 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 37 - 208 4.8e-42 NAD binding domain of 6-phosphogluconate dehydrogenase
F420_oxidored PF03807 37 - 101 2.4e-07 NADP oxidoreductase coenzyme F420-dependent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 237, 521, 575
AclWI GGATC 4 cut(s) 135, 148, 392, 562
AcsI RAATTY 3 cut(s) 60, 358, 725
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 2 cut(s) 14, 442
AfiI CCNNNNNNNGG 3 cut(s) 503, 520, 598
AloI GAACNNNNNNTCC 2 cut(s) 573, 605
AluBI AGCT 6 cut(s) 185, 272, 418, 527, 566, 685
AluI AGCT 6 cut(s) 185, 272, 418, 527, 566, 685
Alw26I GTCTC 3 cut(s) 97, 101, 436
AlwI GGATC 4 cut(s) 135, 148, 392, 562
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 518, 731
ApoI RAATTY 3 cut(s) 60, 358, 725
Asp700I GAANNNNTTC 3 cut(s) 218, 418, 637
AspS9I GGNCC 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 332
BamHI GGATCC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 530, 718
BccI CCATC 3 cut(s) 434, 592, 659
BceAI ACGGC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 3 cut(s) 97, 101, 436
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 111
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 237, 519, 576, 732
BlsI GCNGC 4 cut(s) 238, 520, 577, 733
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 142, 235
BmrFI CCNGG 1 cut(s) 69
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 481, 671
BmuI ACTGGG 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 68, 368
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 520, 598
Bse1I ACTGG 2 cut(s) 132, 334
Bse3DI GCAATG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 68, 368
BseGI GGATG 2 cut(s) 386, 496
BseLI CCNNNNNNNGG 3 cut(s) 503, 520, 598
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 549, 726
BseNI ACTGG 2 cut(s) 132, 334
BseXI GCAGC 2 cut(s) 530, 718
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 68, 171
BslFI GGGAC 2 cut(s) 82, 544
BslI CCNNNNNNNGG 3 cut(s) 503, 520, 598
BsmAI GTCTC 3 cut(s) 97, 101, 436
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 2 cut(s) 82, 544
BsnI GGCC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 5 cut(s) 23, 140, 397, 463, 554
BspACI CCGC 4 cut(s) 5, 237, 521, 575
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 2 cut(s) 550, 727
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 142, 235
BspPI GGATC 4 cut(s) 135, 148, 392, 562
BsrDI GCAATG 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 2 cut(s) 132, 334
BssECI CCNNGG 2 cut(s) 68, 368
BssMI GATC 5 cut(s) 23, 140, 397, 463, 554
Bst4CI ACNGT 3 cut(s) 112, 440, 613
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 4 cut(s) 558, 706, 713, 735
BstDSI CCRYGG 1 cut(s) 368
BstF5I GGATG 2 cut(s) 386, 496
BstKTI GATC 5 cut(s) 26, 143, 400, 466, 557
BstMAI GTCTC 3 cut(s) 97, 101, 436
BstMBI GATC 5 cut(s) 23, 140, 397, 463, 554
BstMWI GCNNNNNNNGC 4 cut(s) 370, 484, 524, 572
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 530, 718
BstX2I RGATCY 2 cut(s) 140, 554
BstYI RGATCY 2 cut(s) 140, 554
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 170
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 2 cut(s) 386, 496
Cac8I GCNNGC 1 cut(s) 480
CciI TCATGA 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 2 cut(s) 13, 441
CviAII CATG 5 cut(s) 191, 211, 311, 544, 702
CviQI GTAC 2 cut(s) 13, 441
DdeI CTNAG 4 cut(s) 558, 706, 713, 735
DpnI GATC 5 cut(s) 25, 142, 399, 465, 556
DpnII GATC 5 cut(s) 23, 140, 397, 463, 554
Eco47I GGWCC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 436
FaeI CATG 5 cut(s) 194, 214, 314, 547, 705
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 82, 544
FatI CATG 5 cut(s) 190, 210, 310, 543, 701
Fnu4HI GCNGC 4 cut(s) 237, 519, 576, 732
FokI GGATG 2 cut(s) 373, 503
Fsp4HI GCNGC 4 cut(s) 237, 519, 576, 732
FspBI CTAG 1 cut(s) 273
GluI GCNGC 4 cut(s) 237, 519, 576, 732
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 1 cut(s) 170
HapII CCGG 2 cut(s) 68, 171
Hin1II CATG 5 cut(s) 194, 214, 314, 547, 705
HindIII AAGCTT 2 cut(s) 416, 564
HinfI GANTC 2 cut(s) 383, 532
HpaII CCGG 2 cut(s) 68, 171
Hpy166II GTNNAC 2 cut(s) 344, 694
Hpy188I TCNGA 2 cut(s) 226, 559
Hpy188III TCNNGA 5 cut(s) 144, 191, 411, 504, 722
Hpy8I GTNNAC 2 cut(s) 344, 694
HpyCH4III ACNGT 3 cut(s) 112, 440, 613
HpyCH4V TGCA 4 cut(s) 204, 266, 518, 662
HpyF10VI GCNNNNNNNGC 4 cut(s) 370, 484, 524, 572
HpyF3I CTNAG 4 cut(s) 558, 706, 713, 735
Hsp92II CATG 5 cut(s) 194, 214, 314, 547, 705
Kzo9I GATC 5 cut(s) 23, 140, 397, 463, 554
LmnI GCTCC 3 cut(s) 233, 300, 499
Lsp1109I GCAGC 2 cut(s) 530, 718
LweI GCATC 2 cut(s) 481, 671
MaeI CTAG 1 cut(s) 273
MalI GATC 5 cut(s) 25, 142, 399, 465, 556
MboI GATC 5 cut(s) 23, 140, 397, 463, 554
MboII GAAGA 3 cut(s) 473, 484, 616
MflI RGATCY 2 cut(s) 140, 554
MluCI AATT 4 cut(s) 60, 101, 358, 725
MlyI GAGTC 1 cut(s) 526
MnlI CCTC 3 cut(s) 347, 500, 553
MroXI GAANNNNTTC 3 cut(s) 218, 418, 637
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 68, 171
MspR9I CCNGG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 370, 484, 524, 572
NciI CCSGG 1 cut(s) 69
NdeII GATC 5 cut(s) 23, 140, 397, 463, 554
NlaIII CATG 5 cut(s) 194, 214, 314, 547, 705
NlaIV GGNNCC 2 cut(s) 142, 235
NmeAIII GCCGAG 1 cut(s) 62
PagI TCATGA 1 cut(s) 190
PdmI GAANNNNTTC 3 cut(s) 218, 418, 637
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 4 cut(s) 238, 520, 577, 733
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 527
PspN4I GGNNCC 2 cut(s) 142, 235
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 2 cut(s) 140, 554
RsaI GTAC 2 cut(s) 14, 442
RsaNI GTAC 2 cut(s) 13, 441
RseI CAYNNNNRTG 1 cut(s) 315
SatI GCNGC 4 cut(s) 237, 519, 576, 732
Sau3AI GATC 5 cut(s) 23, 140, 397, 463, 554
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 69
SetI ASST 8 cut(s) 187, 256, 274, 420, 511, 529, 568, 687
SfaNI GCATC 2 cut(s) 481, 671
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 4 cut(s) 60, 101, 358, 725
SsiI CCGC 4 cut(s) 5, 237, 521, 575
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 3 cut(s) 112, 440, 613
TaqI TCGA 2 cut(s) 410, 596
TasI AATT 4 cut(s) 60, 101, 358, 725
TatI WGTACW 2 cut(s) 12, 440
TauI GCSGC 2 cut(s) 239, 578
TfiI GAWTC 1 cut(s) 383
TseI GCWGC 2 cut(s) 518, 731
TspDTI ATGAA 5 cut(s) 179, 227, 375, 532, 690
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 3 cut(s) 60, 358, 725
XmnI GAANNNNTTC 3 cut(s) 218, 418, 637
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.