Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011427

Physical Location & Seq

Genomic Coordinates Forward (+)

41450664 .. 41454833

Gene Structure 4170 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1280500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGAAAATGGGCGCCGAAGAAGAAGATAATCAGAAGAGCATACCAGTTCTGCCATGGATGCGAAGCCCAATCGATGTCAGTGCCGTCCACGAATGCCCTCTCTCTCTCCTCCCCTGCATTGATCCCAGGTTGAAGGTGTCGTTGGAGAAGATGGGGATCTCTTCACTTTTCCCAGTTCAGCTTGCTGTTTGGCAGGAGACTGTTGGCCCGGGAGGTTTCGAGCGCGATTTGTGTGTTAATTCTCCAACTGGAAGTGGGAAGACTTTAGCTTATGCATTGCCAATTGTACAAATGCTGTCGACTCGCTCTGTGAAATGTCTTCGTGCGCTCGTGGTGCTGCCGACTCGGGATTTAGCATTGCAGGTGAAAGAGGTTTTTGCTGCAATTGCACCTGCAGTGGGGTTATCTGTTGGTCTTGCAGTTGGCCAATCTTCTATCGCTGATGAAATTTCAGAGCTTATCAATAGGCCTAAGCTTGAGCTGGGTGTTAGATTTGACCCTGACGATGTTTTTCCCGAGTTACGAAGTTCTGTGGACATATTGGTGGCTACCCCAGGAAGGCTAATGGACCATATAAATAACACTAAAGGATTTACACTTGAGCATCTCTGTTATCTTGTTGTTGATGAAACAGATCGATTGCTAAGGGAGGCATACCAGTCTTGGCTACCCACTGTGCTTCAAATCACTCGCCCTGACGATGAAAGTTTCCTTCCCTGTGCTCAAAATTTTCTACCTTCCCCATTTGGTTCCTTAAAAACCATAAGGAGATCTGGTCTTGAGAGGGGGTTTAAGGGTAAATCTTACCCCAGGCTTGTGAAGATGGTTCTGTCAGCCACGCTAACCCAGGATCCAAGCAAACTTGCTCAGCTAGATCTGCATCATCCTTTGTTCTTGACAACTGGACAAAAGCGTTATCAGCTTCCAGAAAAGTTACAGTCCTACAAGCTGTTATGCGAATCAAAATACAAGCCATTATATTTGGTTGGACTCCTGCAAAGTTTAGGAGCAGAGAAGTGCCTCATTTTCACATCCTCTGTGGAGTCAACTCATCGTCTATGCACCTTATTGAACTCTTTTGATCATCTGCCTTTCAAGATTAAGGAGTATTCAAGCCTTCAACGTCAATCTATTCGAAGCAAGACATTGAAGGCATTTCGGAAAGGGGAGATTCAAGTACTCGTTTCATCTGATGCAATGACTCGTGGAATGGATGTTGAAGGGGTGAGGAATGTCATTAATTATGATATGCCTCCATACATAAAGACATACATACACCGTGCAGGTCGGACAGCAAGAGCAGGCCAAAATGGTTGTTGCTTCACACTGTTGCGTAAGGAAGAGGTTAAGCGTTTCAACAAGCTATTACGCAAAGCTGAGAATAACTCTTGCTCTAAATACTCTGTTCCTTCTAGTTACATTGAACCACTCCACTCTATCTATGAATCCGCACTGGAGAAATTGAAAGAGACTGTTGAAATGGAAACATCCCGGAAGAGAAAGCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017151 GO:0019899 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

503

Amino Acids

56.41

Weight (kDa)

9.14

Isoelectric Point (pI)

50.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	58 - 283	6.4e-28	DEAD/DEAH box helicase
ResIII	PF04851	74 - 220	6.9e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	327 - 435	1.9e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15850
fragaria_vesca	FvH4_3g05121 FvH4_3g05121
malus_domestica	MD05G1280500.v1.1
prunus_persica	Prupe.4G084900_v2.0.a1 Prupe.4G084900_v2.0.a1 Prupe.4G084900_v2.0.a1 Prupe.4G084900_v2.0.a1
pyrus_communis	pycom05g26080
rosa_chinensis	RchiOBHm_Chr5g0014641
rosa_laevigata	RLG00000032134
rosa_multiflora	Rmu_sc0000120.1_g000019
rosa_roxburghii	Rroxscaffold_1G00061820
rosa_rugosa	Rorug05G0019900 Rorug05G0020000
rosa_samantha	Rh5AG115600 Rh5BG112200 Rh5CG123800 Rh5DG112000
rosa_wichuraiana	Rw5G009980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	352, 400
Acc36I	ACCTGC	3 cut(s)	352, 400, 1274
AccB1I	GGYRCC	1 cut(s)	11
AccI	GTMKAC	1 cut(s)	299
AccII	CGCG	1 cut(s)	225
AciI	CCGC	2 cut(s)	1449, 1504
AclWI	GGATC	4 cut(s)	116, 164, 845, 858
AcoI	YGGCCR	1 cut(s)	424
AcsI	RAATTY	2 cut(s)	447, 727
AcyI	GRCGYC	1 cut(s)	12
AfaI	GTAC	2 cut(s)	288, 1179
AfiI	CCNNNNNNNGG	2 cut(s)	398, 558
AjnI	CCWGG	4 cut(s)	125, 553, 809, 846
AjuI	GAANNNNNNNTTGG	2 cut(s)	125, 157
Alw21I	GWGCWC	1 cut(s)	724
Alw26I	GTCTC	2 cut(s)	191, 1463
AlwI	GGATC	4 cut(s)	116, 164, 845, 858
Ama87I	CYCGRG	3 cut(s)	208, 345, 515
AoxI	GGCC	4 cut(s)	205, 424, 467, 1303
ApeKI	GCWGC	2 cut(s)	337, 380
ApoI	RAATTY	2 cut(s)	447, 727
AseI	ATTAAT	1 cut(s)	1239
AspLEI	GCGC	3 cut(s)	14, 225, 328
AspS9I	GGNCC	2 cut(s)	206, 568
AsuC2I	CCSGG	3 cut(s)	209, 210, 1492
AsuHPI	GGTGA	2 cut(s)	376, 1237
AsuII	TTCGAA	1 cut(s)	1135
AvaI	CYCGRG	3 cut(s)	208, 345, 515
AvaII	GGWCC	1 cut(s)	568
BalI	TGGCCA	1 cut(s)	426
BamHI	GGATCC	1 cut(s)	850
BanI	GGYRCC	1 cut(s)	11
BauI	CACGAG	2 cut(s)	329, 1203
BbsI	GAAGAC	2 cut(s)	266, 311
Bbv12I	GWGCWC	1 cut(s)	724
BbvI	GCAGC	2 cut(s)	324, 367
BccI	CCATC	2 cut(s)	145, 817
BceAI	ACGGC	1 cut(s)	68
BcgI	CGANNNNNNTGC	2 cut(s)	62, 96
BciT130I	CCWGG	4 cut(s)	127, 555, 811, 848
BclI	TGATCA	1 cut(s)	1081
BcnI	CCSGG	3 cut(s)	209, 210, 1492
BcoDI	GTCTC	2 cut(s)	191, 1463
BfaI	CTAG	2 cut(s)	872, 1413
BfmI	CTRYAG	1 cut(s)	393
BfoI	RGCGCY	1 cut(s)	15
BfuAI	ACCTGC	3 cut(s)	352, 400, 1274
BglII	AGATCT	2 cut(s)	770, 874
BisI	GCNGC	2 cut(s)	338, 381
BlpI	GCTNAGC	1 cut(s)	867
BlsI	GCNGC	2 cut(s)	339, 382
BmcAI	AGTACT	1 cut(s)	1179
Bme1390I	CCNGG	7 cut(s)	127, 209, 210, 555, 811, 848, 1492
Bme18I	GGWCC	1 cut(s)	568
BmeT110I	CYCGRG	3 cut(s)	208, 345, 515
BmgT120I	GGNCC	2 cut(s)	206, 568
BmiI	GGNNCC	3 cut(s)	13, 751, 852
BmrFI	CCNGG	7 cut(s)	127, 209, 210, 555, 811, 848, 1492
BmrI	ACTGGG	1 cut(s)	167
BmsI	GCATC	4 cut(s)	48, 613, 889, 1183
BmuI	ACTGGG	1 cut(s)	167
BpiI	GAAGAC	2 cut(s)	266, 311
BplI	GAGNNNNNCTC	2 cut(s)	1370, 1402
BpmI	CTGGAG	1 cut(s)	1475
Bpu10I	CCTNAGC	2 cut(s)	471, 644
Bpu1102I	GCTNAGC	1 cut(s)	867
Bpu14I	TTCGAA	1 cut(s)	1135
BpuEI	CTTGAG	3 cut(s)	497, 620, 800
BpuMI	CCSGG	3 cut(s)	209, 210, 1492
Bsa29I	ATCGAT	2 cut(s)	72, 637
BsaBI	GATNNNNATC	2 cut(s)	155, 879
BsaHI	GRCGYC	1 cut(s)	12
BsaJI	CCNNGG	6 cut(s)	53, 125, 208, 553, 809, 846
BsaXI	ACNNNNNCTCC	2 cut(s)	760, 790
Bsc4I	CCNNNNNNNGG	2 cut(s)	398, 558
Bse1I	ACTGG	6 cut(s)	44, 173, 253, 658, 907, 1458
Bse3DI	GCAATG	3 cut(s)	275, 356, 1203
Bse8I	GATNNNNATC	2 cut(s)	155, 879
BseBI	CCWGG	4 cut(s)	127, 555, 811, 848
BseCI	ATCGAT	2 cut(s)	72, 637
BseDI	CCNNGG	6 cut(s)	53, 125, 208, 553, 809, 846
BseGI	GGATG	5 cut(s)	63, 883, 1031, 1219, 1487
BseJI	GATNNNNATC	2 cut(s)	155, 879
BseLI	CCNNNNNNNGG	2 cut(s)	398, 558
BseMI	GCAATG	3 cut(s)	275, 356, 1203
BseMII	CTCAG	2 cut(s)	881, 1368
BseNI	ACTGG	6 cut(s)	44, 173, 253, 658, 907, 1458
BseRI	GAGGAG	1 cut(s)	98
BseXI	GCAGC	2 cut(s)	324, 367
BseYI	CCCAGC	1 cut(s)	481
BsgI	GTGCAG	1 cut(s)	1302
Bsh1236I	CGCG	1 cut(s)	225
BshFI	GGCC	4 cut(s)	207, 426, 469, 1305
BshNI	GGYRCC	1 cut(s)	11
BshVI	ATCGAT	2 cut(s)	72, 637
BsiHKAI	GWGCWC	1 cut(s)	724
BsiHKCI	CYCGRG	3 cut(s)	208, 345, 515
BsiSI	CCGG	2 cut(s)	209, 1492
BslI	CCNNNNNNNGG	2 cut(s)	398, 558
BsmAI	GTCTC	2 cut(s)	191, 1463
BsmI	GAATGC	1 cut(s)	98
BsnI	GGCC	4 cut(s)	207, 426, 469, 1305
BsoBI	CYCGRG	3 cut(s)	208, 345, 515
Bsp119I	TTCGAA	1 cut(s)	1135
Bsp1286I	GDGCHC	1 cut(s)	724
Bsp1407I	TGTACA	1 cut(s)	286
Bsp143I	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
Bsp1720I	GCTNAGC	1 cut(s)	867
Bsp19I	CCATGG	1 cut(s)	53
BspACI	CCGC	2 cut(s)	1449, 1504
BspANI	GGCC	4 cut(s)	207, 426, 469, 1305
BspCNI	CTCAG	2 cut(s)	880, 1369
BspDI	ATCGAT	2 cut(s)	72, 637
BspFNI	CGCG	1 cut(s)	225
BspLI	GGNNCC	3 cut(s)	13, 751, 852
BspMAI	CTGCAG	1 cut(s)	397
BspMI	ACCTGC	3 cut(s)	352, 400, 1274
BspPI	GGATC	4 cut(s)	116, 164, 845, 858
BspQI	GCTCTTC	1 cut(s)	29
BspT104I	TTCGAA	1 cut(s)	1135
BspT107I	GGYRCC	1 cut(s)	11
BsrDI	GCAATG	3 cut(s)	275, 356, 1203
BsrGI	TGTACA	1 cut(s)	286
BsrI	ACTGG	6 cut(s)	44, 173, 253, 658, 907, 1458
BssECI	CCNNGG	6 cut(s)	53, 125, 208, 553, 809, 846
BssMI	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
BssNI	GRCGYC	1 cut(s)	12
BssSI	CACGAG	2 cut(s)	329, 1203
BssT1I	CCWWGG	1 cut(s)	53
Bst2BI	CACGAG	2 cut(s)	329, 1203
Bst2UI	CCWGG	4 cut(s)	127, 555, 811, 848
Bst4CI	ACNGT	6 cut(s)	202, 676, 939, 1280, 1329, 1474
Bst6I	CTCTTC	4 cut(s)	29, 166, 1335, 1490
BstACI	GRCGYC	1 cut(s)	12
BstAUI	TGTACA	1 cut(s)	286
BstBI	TTCGAA	1 cut(s)	1135
BstC8I	GCNNGC	2 cut(s)	183, 1303
BstDEI	CTNAG	4 cut(s)	471, 644, 867, 1377
BstDSI	CCRYGG	1 cut(s)	53
BstF5I	GGATG	5 cut(s)	63, 883, 1031, 1219, 1487
BstFNI	CGCG	1 cut(s)	225
BstH2I	RGCGCY	1 cut(s)	15
BstHHI	GCGC	3 cut(s)	14, 225, 328
BstKTI	GATC	7 cut(s)	124, 159, 637, 773, 853, 877, 1084
BstMAI	GTCTC	2 cut(s)	191, 1463
BstMBI	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
BstMWI	GCNNNNNNNGC	5 cut(s)	58, 334, 386, 877, 919
BstNI	CCWGG	4 cut(s)	127, 555, 811, 848
BstSCI	CCNGG	7 cut(s)	125, 207, 208, 553, 809, 846, 1490
BstSFI	CTRYAG	1 cut(s)	393
BstUI	CGCG	1 cut(s)	225
BstV1I	GCAGC	2 cut(s)	324, 367
BstV2I	GAAGAC	2 cut(s)	266, 311
BstX2I	RGATCY	4 cut(s)	156, 770, 850, 874
BstYI	RGATCY	4 cut(s)	156, 770, 850, 874
Bsu15I	ATCGAT	2 cut(s)	72, 637
BsuRI	GGCC	4 cut(s)	207, 426, 469, 1305
BsuTUI	ATCGAT	2 cut(s)	72, 637
BtgI	CCRYGG	1 cut(s)	53
BtsCI	GGATG	5 cut(s)	63, 883, 1031, 1219, 1487
BtsI	GCAGTG	1 cut(s)	402
BtsIMutI	CAGTG	5 cut(s)	85, 402, 672, 1325, 1451
BveI	ACCTGC	3 cut(s)	352, 400, 1274
Cac8I	GCNNGC	2 cut(s)	183, 1303
CfoI	GCGC	3 cut(s)	14, 225, 328
Cfr13I	GGNCC	2 cut(s)	206, 568
Cfr9I	CCCGGG	1 cut(s)	208
ClaI	ATCGAT	2 cut(s)	72, 637
Csp6I	GTAC	2 cut(s)	287, 1178
CviAII	CATG	1 cut(s)	54
CviQI	GTAC	2 cut(s)	287, 1178
DdeI	CTNAG	4 cut(s)	471, 644, 867, 1377
DinI	GGCGCC	1 cut(s)	13
DpnI	GATC	7 cut(s)	123, 158, 636, 772, 852, 876, 1083
DpnII	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
EaeI	YGGCCR	1 cut(s)	424
Eam1104I	CTCTTC	4 cut(s)	29, 166, 1335, 1490
EarI	CTCTTC	4 cut(s)	29, 166, 1335, 1490
Eco130I	CCWWGG	1 cut(s)	53
Eco147I	AGGCCT	1 cut(s)	469
Eco47I	GGWCC	1 cut(s)	568
Eco88I	CYCGRG	3 cut(s)	208, 345, 515
EcoRII	CCWGG	4 cut(s)	125, 553, 809, 846
EcoT14I	CCWWGG	1 cut(s)	53
EcoT22I	ATGCAT	1 cut(s)	277
EgeI	GGCGCC	1 cut(s)	13
EheI	GGCGCC	1 cut(s)	13
ErhI	CCWWGG	1 cut(s)	53
FaeI	CATG	1 cut(s)	57
FatI	CATG	1 cut(s)	53
FbaI	TGATCA	1 cut(s)	1081
FblI	GTMKAC	1 cut(s)	299
Fnu4HI	GCNGC	2 cut(s)	338, 381
FokI	GGATG	5 cut(s)	70, 870, 1018, 1226, 1474
Fsp4HI	GCNGC	2 cut(s)	338, 381
FspBI	CTAG	2 cut(s)	872, 1413
GlaI	GCGC	3 cut(s)	13, 224, 327
GluI	GCNGC	2 cut(s)	338, 381
GsaI	CCCAGC	1 cut(s)	485
GsuI	CTGGAG	1 cut(s)	1475
HaeII	RGCGCY	1 cut(s)	15
HaeIII	GGCC	4 cut(s)	207, 426, 469, 1305
HapII	CCGG	2 cut(s)	209, 1492
HhaI	GCGC	3 cut(s)	14, 225, 328
Hin1I	GRCGYC	1 cut(s)	12
Hin1II	CATG	1 cut(s)	57
Hin6I	GCGC	3 cut(s)	12, 223, 326
HinP1I	GCGC	3 cut(s)	12, 223, 326
HincII	GTYRAC	2 cut(s)	300, 1047
HindII	GTYRAC	2 cut(s)	300, 1047
HindIII	AAGCTT	1 cut(s)	473
HinfI	GANTC	8 cut(s)	301, 343, 959, 990, 1043, 1171, 1201, 1445
HpaII	CCGG	2 cut(s)	209, 1492
HphI	GGTGA	2 cut(s)	376, 1237
Hpy166II	GTNNAC	4 cut(s)	88, 300, 535, 1047
Hpy188I	TCNGA	5 cut(s)	33, 454, 1161, 1192, 1290
Hpy188III	TCNNGA	6 cut(s)	347, 515, 779, 895, 926, 1096
Hpy8I	GTNNAC	4 cut(s)	88, 300, 535, 1047
HpyAV	CCTTC	8 cut(s)	127, 552, 722, 747, 1127, 1144, 1214, 1419
HpyCH4III	ACNGT	6 cut(s)	202, 676, 939, 1280, 1329, 1474
HpyCH4IV	ACGT	1 cut(s)	1123
HpyF10VI	GCNNNNNNNGC	5 cut(s)	58, 334, 386, 877, 919
HpyF3I	CTNAG	4 cut(s)	471, 644, 867, 1377
HpySE526I	ACGT	1 cut(s)	1123
Hsp92I	GRCGYC	1 cut(s)	12
Hsp92II	CATG	1 cut(s)	57
HspAI	GCGC	3 cut(s)	12, 223, 326
KasI	GGCGCC	1 cut(s)	11
Ksp22I	TGATCA	1 cut(s)	1081
Kzo9I	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
LguI	GCTCTTC	1 cut(s)	29
LmnI	GCTCC	1 cut(s)	1007
Lsp1109I	GCAGC	2 cut(s)	324, 367
LweI	GCATC	4 cut(s)	48, 613, 889, 1183
MaeI	CTAG	2 cut(s)	872, 1413
MaeII	ACGT	1 cut(s)	1123
MaeIII	GTNAC	3 cut(s)	519, 933, 1415
MalI	GATC	7 cut(s)	123, 158, 636, 772, 852, 876, 1083
MboI	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
MfeI	CAATTG	2 cut(s)	282, 384
MflI	RGATCY	4 cut(s)	156, 770, 850, 874
MhlI	GDGCHC	1 cut(s)	724
MlsI	TGGCCA	1 cut(s)	426
MluCI	AATT	7 cut(s)	238, 282, 384, 447, 727, 1240, 1460
MluNI	TGGCCA	1 cut(s)	426
Mly113I	GGCGCC	1 cut(s)	12
MlyI	GAGTC	5 cut(s)	295, 337, 984, 1052, 1195
MmeI	TCCRAC	4 cut(s)	123, 269, 967, 1268
Mox20I	TGGCCA	1 cut(s)	426
Mph1103I	ATGCAT	1 cut(s)	277
MscI	TGGCCA	1 cut(s)	426
MseI	TTAA	6 cut(s)	237, 755, 792, 1101, 1239, 1347
MslI	CAYNNNNRTG	1 cut(s)	542
Msp20I	TGGCCA	1 cut(s)	426
MspI	CCGG	2 cut(s)	209, 1492
MspR9I	CCNGG	7 cut(s)	127, 209, 210, 555, 811, 848, 1492
MunI	CAATTG	2 cut(s)	282, 384
Mva1269I	GAATGC	1 cut(s)	98
MvaI	CCWGG	4 cut(s)	127, 555, 811, 848
MvnI	CGCG	1 cut(s)	225
MwoI	GCNNNNNNNGC	5 cut(s)	58, 334, 386, 877, 919
NarI	GGCGCC	1 cut(s)	12
NciI	CCSGG	3 cut(s)	209, 210, 1492
NcoI	CCATGG	1 cut(s)	53
NdeII	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
NlaIII	CATG	1 cut(s)	57
NlaIV	GGNNCC	3 cut(s)	13, 751, 852
NsiI	ATGCAT	1 cut(s)	277
NspV	TTCGAA	1 cut(s)	1135
PaqCI	CACCTGC	2 cut(s)	352, 400
PceI	AGGCCT	1 cut(s)	469
PciSI	GCTCTTC	1 cut(s)	29
PctI	GAATGC	1 cut(s)	98
PfeI	GAWTC	3 cut(s)	959, 1171, 1445
PfoI	TCCNGGA	1 cut(s)	1490
PkrI	GCNGC	2 cut(s)	339, 382
PleI	GAGTC	5 cut(s)	295, 337, 984, 1051, 1195
PluTI	GGCGCC	1 cut(s)	15
PpsI	GAGTC	5 cut(s)	295, 337, 984, 1051, 1195
PshBI	ATTAAT	1 cut(s)	1239
Psp6I	CCWGG	4 cut(s)	125, 553, 809, 846
PspFI	CCCAGC	1 cut(s)	481
PspGI	CCWGG	4 cut(s)	125, 553, 809, 846
PspN4I	GGNNCC	3 cut(s)	13, 751, 852
PspPI	GGNCC	2 cut(s)	206, 568
PstI	CTGCAG	1 cut(s)	397
PsuI	RGATCY	4 cut(s)	156, 770, 850, 874
RsaI	GTAC	2 cut(s)	288, 1179
RsaNI	GTAC	2 cut(s)	287, 1178
RseI	CAYNNNNRTG	1 cut(s)	542
SalI	GTCGAC	1 cut(s)	298
SapI	GCTCTTC	1 cut(s)	29
SaqAI	TTAA	6 cut(s)	237, 755, 792, 1101, 1239, 1347
SatI	GCNGC	2 cut(s)	338, 381
Sau3AI	GATC	7 cut(s)	121, 156, 634, 770, 850, 874, 1081
Sau96I	GGNCC	2 cut(s)	206, 568
ScaI	AGTACT	1 cut(s)	1179
SchI	GAGTC	5 cut(s)	295, 337, 984, 1052, 1195
ScrFI	CCNGG	7 cut(s)	127, 209, 210, 555, 811, 848, 1492
SduI	GDGCHC	1 cut(s)	724
SfaNI	GCATC	4 cut(s)	48, 613, 889, 1183
SfcI	CTRYAG	1 cut(s)	393
SfoI	GGCGCC	1 cut(s)	13
SfuI	TTCGAA	1 cut(s)	1135
SinI	GGWCC	1 cut(s)	568
SmaI	CCCGGG	1 cut(s)	210
SmiMI	CAYNNNNRTG	1 cut(s)	542
SmlI	CTYRAG	3 cut(s)	476, 599, 779
SmoI	CTYRAG	3 cut(s)	476, 599, 779
Sse9I	AATT	7 cut(s)	238, 282, 384, 447, 727, 1240, 1460
SseBI	AGGCCT	1 cut(s)	469
SsiI	CCGC	2 cut(s)	1449, 1504
SspDI	GGCGCC	1 cut(s)	11
SspMI	CTAG	2 cut(s)	872, 1413
StuI	AGGCCT	1 cut(s)	469
StyD4I	CCNGG	7 cut(s)	125, 207, 208, 553, 809, 846, 1490
StyI	CCWWGG	1 cut(s)	53
TaaI	ACNGT	6 cut(s)	202, 676, 939, 1280, 1329, 1474
TaiI	ACGT	1 cut(s)	1126
TaqI	TCGA	5 cut(s)	72, 219, 299, 637, 1135
TasI	AATT	7 cut(s)	238, 282, 384, 447, 727, 1240, 1460
TatI	WGTACW	2 cut(s)	286, 1177
TfiI	GAWTC	3 cut(s)	959, 1171, 1445
Tru1I	TTAA	6 cut(s)	237, 755, 792, 1101, 1239, 1347
Tru9I	TTAA	6 cut(s)	237, 755, 792, 1101, 1239, 1347
TscAI	CASTG	5 cut(s)	85, 402, 679, 1332, 1458
TseI	GCWGC	2 cut(s)	337, 380
TspDTI	ATGAA	6 cut(s)	17, 459, 642, 717, 1176, 1458
TspMI	CCCGGG	1 cut(s)	208
TspRI	CASTG	5 cut(s)	85, 402, 679, 1332, 1458
VpaK11BI	GGWCC	1 cut(s)	568
VspI	ATTAAT	1 cut(s)	1239
XapI	RAATTY	2 cut(s)	447, 727
XcmI	CCANNNNNNNNNTGG	1 cut(s)	51
XmaI	CCCGGG	1 cut(s)	208
XmiI	GTMKAC	1 cut(s)	299
XspI	CTAG	2 cut(s)	872, 1413
ZrmI	AGTACT	1 cut(s)	1179
Zsp2I	ATGCAT	1 cut(s)	277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.