MD05G1284500.v1.1

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011410
Physical Location & Seq
Reverse (-)
41842451 .. 41844985
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1284500.v1.1.491

Sequence Viewer

Length: 519 bp
ATGACCCAACATAATTCACAGCCCAAAATCATACCGAACCGGAACCGGATCTCAAACAAAAAACCGGAAAGAAAGGCGAGAGGCCTGAGCTCCTGCGCAGAAGCGGCAGCAGATAGCAGCAAATCAATGGCGTCGTCGTCAGCTCTTCGAGAGCTTCAGCGCGAATTGGAGGCCAAAGCTAACGATCTCAGCAAAATCCAAAAGGATATTTCAAAGAATCACCAAGTGAGGAAGAAGTACACTATCCAGCTCGGCGAGAACGAGCTCGTCCTCAAGGAATTGGATCTTCTTAGCGAAGACGCCAATGTGTTCAAGTTGATCGGTCCGGTTCTTGTGAAGCAGGATTTGGCAGAGGCTAGGGCCAATGTGCGAAAGAGAATCGAATACATCTCCGCTGAATTGAAACGGCTGGATGCTACCCTTCAAGATTTGGAAGAGAAGCAAAATAGCAAGAAAGAGACGATTTTAAAATTACAACAGAGGGCTCAAGCACTCCAGGCCGGAAAAGCGAAGGCATAA

Protein Analysis

173

Amino Acids

19.34

Weight (kDa)

9.78

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 58 - 161 1.6e-22 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 97
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 104, 393
AclWI GGATC 2 cut(s) 56, 291
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 2 cut(s) 131, 300
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 1 cut(s) 239
AgsI TTSAA 4 cut(s) 213, 313, 403, 425
AjnI CCWGG 1 cut(s) 495
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 90, 143, 154, 179, 250, 265
AluI AGCT 6 cut(s) 90, 143, 154, 179, 250, 265
Alw21I GWGCWC 2 cut(s) 92, 267
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 56, 291
AoxI GGCC 4 cut(s) 82, 171, 360, 498
ApeKI GCWGC 2 cut(s) 107, 117
AspLEI GCGC 2 cut(s) 98, 162
AspS9I GGNCC 2 cut(s) 323, 360
AsuHPI GGTGA 1 cut(s) 212
AvaII GGWCC 1 cut(s) 323
BanII GRGCYC 3 cut(s) 92, 267, 487
BbsI GAAGAC 1 cut(s) 303
Bbv12I GWGCWC 2 cut(s) 92, 267
BbvI GCAGC 2 cut(s) 119, 129
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 1 cut(s) 497
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 357
BisI GCNGC 3 cut(s) 105, 108, 118
BlsI GCNGC 3 cut(s) 106, 109, 119
Bme1390I CCNGG 1 cut(s) 497
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 323, 360
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 497
BmsI GCATC 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 257, 471
BsaHI GRCGYC 2 cut(s) 131, 300
BsaWI WCCGGW 4 cut(s) 39, 45, 64, 325
BseBI CCWGG 1 cut(s) 497
BseGI GGATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 77, 202
BseXI GCAGC 2 cut(s) 119, 129
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 4 cut(s) 84, 173, 362, 500
BsiHKAI GWGCWC 2 cut(s) 92, 267
BsiSI CCGG 5 cut(s) 40, 46, 65, 326, 501
BsmAI GTCTC 1 cut(s) 452
BsmBI CGTCTC 1 cut(s) 452
BsnI GGCC 4 cut(s) 84, 173, 362, 500
Bsp1286I GDGCHC 3 cut(s) 92, 267, 487
Bsp143I GATC 4 cut(s) 48, 184, 283, 318
BspACI CCGC 2 cut(s) 104, 393
BspANI GGCC 4 cut(s) 84, 173, 362, 500
BspCNI CTCAG 2 cut(s) 78, 201
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 2 cut(s) 56, 291
BspQI GCTCTTC 1 cut(s) 150
BssMI GATC 4 cut(s) 48, 184, 283, 318
BssNI GRCGYC 2 cut(s) 131, 300
Bst2UI CCWGG 1 cut(s) 497
Bst6I CTCTTC 2 cut(s) 150, 429
BstACI GRCGYC 2 cut(s) 131, 300
BstDEI CTNAG 3 cut(s) 86, 188, 290
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 98, 162
BstKTI GATC 4 cut(s) 51, 187, 286, 321
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 4 cut(s) 48, 184, 283, 318
BstMWI GCNNNNNNNGC 3 cut(s) 104, 497, 506
BstNI CCWGG 1 cut(s) 497
BstSCI CCNGG 1 cut(s) 495
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 119, 129
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 2 cut(s) 48, 283
BstYI RGATCY 2 cut(s) 48, 283
BsuRI GGCC 4 cut(s) 84, 173, 362, 500
BtsCI GGATG 1 cut(s) 418
CfoI GCGC 2 cut(s) 98, 162
Cfr13I GGNCC 2 cut(s) 323, 360
CpoI CGGWCCG 1 cut(s) 323
CseI GACGC 2 cut(s) 120, 308
Csp6I GTAC 1 cut(s) 238
CspI CGGWCCG 1 cut(s) 323
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 3 cut(s) 86, 188, 290
DpnI GATC 4 cut(s) 50, 186, 285, 320
DpnII GATC 4 cut(s) 48, 184, 283, 318
DraI TTTAAA 1 cut(s) 468
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 150, 429
EarI CTCTTC 2 cut(s) 150, 429
Ecl136II GAGCTC 2 cut(s) 90, 265
Eco147I AGGCCT 1 cut(s) 84
Eco24I GRGCYC 3 cut(s) 92, 267, 487
Eco47I GGWCC 1 cut(s) 323
Eco53kI GAGCTC 2 cut(s) 90, 265
Eco57I CTGAAG 1 cut(s) 140
EcoICRI GAGCTC 2 cut(s) 90, 265
EcoRII CCWGG 1 cut(s) 495
EcoT38I GRGCYC 3 cut(s) 92, 267, 487
Esp3I CGTCTC 1 cut(s) 452
FaiI YATR 3 cut(s) 12, 32, 517
Fnu4HI GCNGC 3 cut(s) 105, 108, 118
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 3 cut(s) 92, 267, 487
Fsp4HI GCNGC 3 cut(s) 105, 108, 118
FspBI CTAG 1 cut(s) 357
FspI TGCGCA 1 cut(s) 97
GlaI GCGC 2 cut(s) 97, 161
GluI GCNGC 3 cut(s) 105, 108, 118
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 4 cut(s) 84, 173, 362, 500
HapII CCGG 5 cut(s) 40, 46, 65, 326, 501
HgaI GACGC 2 cut(s) 120, 308
HhaI GCGC 2 cut(s) 98, 162
Hin1I GRCGYC 2 cut(s) 131, 300
Hin6I GCGC 2 cut(s) 96, 160
HinP1I GCGC 2 cut(s) 96, 160
HinfI GANTC 2 cut(s) 217, 378
HpaII CCGG 5 cut(s) 40, 46, 65, 326, 501
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 240
Hpy188III TCNNGA 2 cut(s) 149, 425
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 2 cut(s) 136, 139
HpyAV CCTTC 2 cut(s) 431, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 497, 506
HpyF3I CTNAG 3 cut(s) 86, 188, 290
Hsp92I GRCGYC 2 cut(s) 131, 300
HspAI GCGC 2 cut(s) 96, 160
Kzo9I GATC 4 cut(s) 48, 184, 283, 318
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 2 cut(s) 119, 129
LweI GCATC 1 cut(s) 403
MaeI CTAG 1 cut(s) 357
MalI GATC 4 cut(s) 50, 186, 285, 320
MboI GATC 4 cut(s) 48, 184, 283, 318
MboII GAAGA 5 cut(s) 137, 244, 278, 308, 446
MflI RGATCY 2 cut(s) 48, 283
MhlI GDGCHC 3 cut(s) 92, 267, 487
MluCI AATT 5 cut(s) 13, 164, 278, 398, 470
MnlI CCTC 6 cut(s) 74, 163, 222, 281, 346, 474
MseI TTAA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 5 cut(s) 40, 46, 65, 326, 501
MspR9I CCNGG 1 cut(s) 497
MvaI CCWGG 1 cut(s) 497
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 104, 497, 506
NdeII GATC 4 cut(s) 48, 184, 283, 318
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 231
NsbI TGCGCA 1 cut(s) 97
PceI AGGCCT 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 2 cut(s) 217, 378
PkrI GCNGC 3 cut(s) 106, 109, 119
Psp124BI GAGCTC 2 cut(s) 92, 267
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 2 cut(s) 323, 360
PsuI RGATCY 2 cut(s) 48, 283
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
Rsr2I CGGWCCG 1 cut(s) 323
RsrII CGGWCCG 1 cut(s) 323
SacI GAGCTC 2 cut(s) 92, 267
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 3 cut(s) 105, 108, 118
Sau3AI GATC 4 cut(s) 48, 184, 283, 318
Sau96I GGNCC 2 cut(s) 323, 360
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 3 cut(s) 92, 267, 487
SetI ASST 6 cut(s) 92, 145, 156, 181, 252, 267
SfaNI GCATC 1 cut(s) 403
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 272, 486
SmoI CTYRAG 2 cut(s) 272, 486
Sse9I AATT 5 cut(s) 13, 164, 278, 398, 470
SseBI AGGCCT 1 cut(s) 84
SsiI CCGC 2 cut(s) 104, 393
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 2 cut(s) 92, 267
StuI AGGCCT 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 495
TaqI TCGA 2 cut(s) 148, 381
TaqII GACCGA 1 cut(s) 311
TasI AATT 5 cut(s) 13, 164, 278, 398, 470
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 217, 378
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TseI GCWGC 2 cut(s) 107, 117
VpaK11BI GGWCC 1 cut(s) 323
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.