Rroxscaffold_1G00062730

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011410
Physical Location & Seq
Reverse (-)
84775325 .. 84778936
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062730.1

Sequence Viewer

Length: 393 bp
ATGGCGTCGTCGTCAGCTCAACGAGAGCTTCAGCGCGAATTGGAAGCCAAAGCTAACGATCTCAGCAAAATCCAAAAGGACATTTCAAAGAATCATCAAGTGAGAAAGAAGTACACCATTCAGCTCGGCGAGAACGAGCTCGTCCTCAAGGAATTGGATCTGCTGAGCGAAGATGCCAATGTGTTCAAATTGATCGGTCCGGTTCTCGTGAAGCAGGATTTGGCAGAGGCCAGGGCCAATGTGCACAAGAGAATTGAGTACATTTCCGCGGAACTGAAGCGGCTGGATGCTACTCTTCAAGATATGGAAGAGAAGCAAAATAGCAAGAAAGAAGCGATTTTTAAAGTACAACAGAGGATTCAATCTCTCCAGGCTGGAAAAGCAAAGGCATAA

Protein Analysis

130

Amino Acids

14.81

Weight (kDa)

9.27

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 16 - 118 2.6e-22 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 36, 269
AciI CCGC 3 cut(s) 267, 269, 280
AclWI GGATC 1 cut(s) 165
AcuI CTGAAG 2 cut(s) 14, 296
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 113, 260, 348
AgsI TTSAA 4 cut(s) 87, 187, 299, 362
AjnI CCWGG 2 cut(s) 230, 369
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 5 cut(s) 17, 28, 53, 124, 139
AluI AGCT 5 cut(s) 17, 28, 53, 124, 139
Alw21I GWGCWC 2 cut(s) 141, 246
Alw44I GTGCAC 1 cut(s) 242
AlwI GGATC 1 cut(s) 165
AoxI GGCC 2 cut(s) 228, 234
ApaLI GTGCAC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 197, 234
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 246
BanII GRGCYC 1 cut(s) 141
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 141, 246
BciT130I CCWGG 2 cut(s) 232, 371
BisI GCNGC 1 cut(s) 281
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 282
Bme1390I CCNGG 2 cut(s) 232, 371
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 197, 234
BmrFI CCNGG 2 cut(s) 232, 371
BmsI GCATC 2 cut(s) 163, 277
BpmI CTGGAG 1 cut(s) 353
Bpu1102I GCTNAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 231, 267
BsaWI WCCGGW 1 cut(s) 199
BseBI CCWGG 2 cut(s) 232, 371
BseDI CCNNGG 2 cut(s) 231, 267
BseGI GGATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 76, 155
BseSI GKGCMC 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 36, 269
BshFI GGCC 2 cut(s) 230, 236
BsiHKAI GWGCWC 2 cut(s) 141, 246
BsiSI CCGG 1 cut(s) 200
BsnI GGCC 2 cut(s) 230, 236
Bsp1286I GDGCHC 2 cut(s) 141, 246
Bsp143I GATC 3 cut(s) 58, 157, 192
Bsp1720I GCTNAGC 1 cut(s) 164
BspACI CCGC 3 cut(s) 267, 269, 280
BspANI GGCC 2 cut(s) 230, 236
BspCNI CTCAG 2 cut(s) 75, 156
BspFNI CGCG 2 cut(s) 36, 269
BspPI GGATC 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 231, 267
BssMI GATC 3 cut(s) 58, 157, 192
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 232, 371
Bst6I CTCTTC 2 cut(s) 300, 303
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 2 cut(s) 62, 164
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 2 cut(s) 36, 269
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 3 cut(s) 61, 160, 195
BstMBI GATC 3 cut(s) 58, 157, 192
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 2 cut(s) 232, 371
BstSCI CCNGG 2 cut(s) 230, 369
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 2 cut(s) 36, 269
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 2 cut(s) 230, 236
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 292
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 197, 234
Cfr42I CCGCGG 1 cut(s) 270
CpoI CGGWCCG 1 cut(s) 197
Csp6I GTAC 3 cut(s) 112, 259, 347
CspI CGGWCCG 1 cut(s) 197
CviQI GTAC 3 cut(s) 112, 259, 347
DdeI CTNAG 2 cut(s) 62, 164
DpnI GATC 3 cut(s) 60, 159, 194
DpnII GATC 3 cut(s) 58, 157, 192
DraI TTTAAA 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 300, 303
EarI CTCTTC 2 cut(s) 300, 303
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 14, 296
EcoICRI GAGCTC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 230, 369
EcoT38I GRGCYC 1 cut(s) 141
FaiI YATR 2 cut(s) 305, 391
Fnu4HI GCNGC 1 cut(s) 281
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 281
GlaI GCGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 281
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 2 cut(s) 230, 236
HapII CCGG 1 cut(s) 200
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HinfI GANTC 2 cut(s) 91, 358
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 114, 244
Hpy188III TCNNGA 2 cut(s) 208, 299
Hpy8I GTNNAC 2 cut(s) 114, 244
Hpy99I CGWCG 2 cut(s) 10, 13
HpyCH4V TGCA 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 2 cut(s) 62, 164
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 34
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 3 cut(s) 58, 157, 192
LpnPI CCDG 8 cut(s) 200, 213, 217, 244, 269, 356, 360, 383
LweI GCATC 2 cut(s) 163, 277
MalI GATC 3 cut(s) 60, 159, 194
MboI GATC 3 cut(s) 58, 157, 192
MboII GAAGA 3 cut(s) 182, 287, 320
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 141, 246
MluCI AATT 4 cut(s) 38, 152, 188, 252
MnlI CCTC 3 cut(s) 155, 220, 348
MseI TTAA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 232, 371
MvaI CCWGG 2 cut(s) 232, 371
MvnI CGCG 2 cut(s) 36, 269
MwoI GCNNNNNNNGC 1 cut(s) 380
NdeII GATC 3 cut(s) 58, 157, 192
NmeAIII GCCGAG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 2 cut(s) 91, 358
PkrI GCNGC 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 230, 369
PspGI CCWGG 2 cut(s) 230, 369
PspPI GGNCC 2 cut(s) 197, 234
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 3 cut(s) 113, 260, 348
RsaNI GTAC 3 cut(s) 112, 259, 347
Rsr2I CGGWCCG 1 cut(s) 197
RsrII CGGWCCG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 281
Sau3AI GATC 3 cut(s) 58, 157, 192
Sau96I GGNCC 2 cut(s) 197, 234
ScrFI CCNGG 2 cut(s) 232, 371
SduI GDGCHC 2 cut(s) 141, 246
SetI ASST 5 cut(s) 19, 30, 55, 126, 141
SfaNI GCATC 2 cut(s) 163, 277
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 4 cut(s) 38, 152, 188, 252
SsiI CCGC 3 cut(s) 267, 269, 280
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 230, 369
TaqII GACCGA 1 cut(s) 185
TasI AATT 4 cut(s) 38, 152, 188, 252
TatI WGTACW 3 cut(s) 111, 258, 346
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 2 cut(s) 91, 358
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.